CN104436291B - A kind of Vaccarin/Bacterial cellulose dressing treating diabetic foot ulcer - Google Patents
A kind of Vaccarin/Bacterial cellulose dressing treating diabetic foot ulcer Download PDFInfo
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- CN104436291B CN104436291B CN201410648771.1A CN201410648771A CN104436291B CN 104436291 B CN104436291 B CN 104436291B CN 201410648771 A CN201410648771 A CN 201410648771A CN 104436291 B CN104436291 B CN 104436291B
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- vaccarin
- dressing
- diabetic foot
- bacterial cellulose
- foot ulcer
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Abstract
The invention discloses a kind of Vaccarin/Bacterial cellulose dressing treating diabetic foot ulcer.Relate to a kind of bio-medical material and preparing technical field thereof.Including, Vaccarin medicine is dissolved in macromolecule polymer solution, obtains composite solution；Use infusion process, make bacteria cellulose film immerse in Vaccarin/high molecular polymer composite solution；With UV-crosslinked, obtain Vaccarin/bacteria cellulose composite membrane, this dressing is the aquagel membrane that the macromolecule polymer solution containing Vaccarin is compound with Bacterial cellulose, preparation process of the present invention is easy to operate, low cost, and the moist aerogel dressing of the treatment diabetic foot ulcer of preparation has the performances such as the damage of anti-high sugar, moisturizing, absorbing wound exudate and accelerating wound healing.It is applicable to the wound repair such as diabetic foot ulcer, venous leg ulcers, pressure ulcer.
Description
Technical field
The present invention relates to bio-medical material and preparing technical field thereof, particularly to a kind of diabetic foot ulcer for the treatment of
The preparation method of Vaccarin/Bacterial cellulose dressing.
Background technology
In recent years, onset diabetes rate presents the trend of substantial increase in worldwide, and diabetic foot is diabetes
One of chronic complicating diseases that patient is serious.The peripheral neuropathy caused by diabetes causes patients feet to feel to lower or lack
Losing, wound is difficult to discover, thus causes ulcer by patient.If can not timely and effective control ulcer, may result in diabetics
Amputation.In annual non-traumatic amputations patient, about 50% is diabetics.Diabetic foot once making a definite diagnosis, remove the cause of disease,
On the basis of the whole body therapeutic that strict glycemic control, nutrition whole body, improvement circulate, treatment of wounds energetically should be carried out, can effectively promote
Enter wound healing, improve prognosis, reduce disability rate, reduce medical expense.
The correct selection of dressing is the powerful guarantee that diabetic foot ulcer obtains optimum therapeuticing effect.At present, based on tradition
Dressing low cost, raw material sources feature widely, most of hospitals still use traditional dry dressing (gauze/oil yarn) to create
Change dressings in face.But the adhesions such as dry dressing fiber and wound surface cambium, transudate, the wound pain produced when removing dressing and
Damage can cause wound healing time prolongation again.Increase it addition, dry dressing absorbs expense produced by poor, the frequent more change dressings of sepage
Add the limitation the most day by day showing it.Along with going deep into researching wound healing, people recognize that preferable dressing is the most simple
It is for flap coverage, it is necessary to wound healing can be helped, creates the suitable environment promoting wound healing." wet wound is managed
Opinion " birth and development, Medical dressing emerges in multitude and is widely used in the practice of chronic wound care.Research table
Bright, moist dressing has the advantage that 1, is conducive to slough and fibrinous dissolving, promote tissue autolyzed clearly
Wound；2, create low-oxygen environment, promote angeogenesis；3, promote multiple growth factor release and regulate its activity；4, avoid
The secondary damage of health tissues when changing dressings, reduces pain.
Semen Vaccariae is the drying and ripening kind of pinkwort Vaccaria segetalis Vaccaria segetalis (Neck.) Garcke
Son.Semen Vaccariae mainly contains triterpene saponin, flavonoid glycoside, cyclic peptide, lipoid and composition [Li Fan, the Liang Jing such as fatty acid, monosaccharide
Treasure. the progress [J] of Semen Vaccariae. Strait Pharmaceutical Journal, 2007,19 (3): 1-5].King is found by this laboratory early-stage Study
Do not stay row flavonoid glycoside can promote the generation of endotheliocyte, have promote angiogenesis effect [Qiu Liying, Feng Lei, Ma Liping,
Deng. a kind of application with the Vaccarin promoting angiogenesis. application number: 201210512699.0].Find simultaneously
Described Vaccarin Human Umbilical Vein Endothelial Cells has the protective effect of anti-high sugar: sets up high sugar damage model, finds king not after administration
Stay the high dose group (13.76 μm ol/L) of row flavonoid glycoside the cell viability of high sugar induction is had clear improvement [Xie Fengshan, Feng Lei,
Ma Liping. the Vaccarin protective effect [J] to hydrogen peroxide and the Human umbilical vein endothelial cells of height sugar induced damage.
Research and development of natural products .2014,26:1009-1013].
Bacterial cellulose (BC), also known as micro organism cellulose.Bacteria cellulose film has the nanostructured of uniqueness, with other
Wound dressing is compared, Bacterial cellulose dressing have the following characteristics that 1. under humid conditions mechanical strength high, have high toughness
Intensity and degree of hydration；2. pair air-liquid and electrolyte have good permeability；3. there is good water absorption, energy continuous and effective
Absorbing wound exudate and metabolite；4. it is a kind of natural biopolymer, non-immunogenicity, there is good biofacies
Capacitive；5. as a kind of physical barriers, can prevent external infections with wound surface close adhesion.These excellent performances are conducive to
The growth of skin histology and healing, be a kind of novel wound dressing [Tang Weihua, Jia Shiru, Jia Yuanyuan. nano meter biomaterial
Bacterial cellulose is at the applied research [J] of medical domain. biomedical engineering's magazine, and 2014,31 (4): 9270929], people
The aspect tools such as work skin and treatment skin injury have been widely used.
A large amount of basic research about wound surface and clinical treatment demonstrate and provide suitable moist environment into wound surface in recent years
Be conducive to the healing of wound.Novel moist combine dressing can keep the moistening of wound surface, can promote again the healing of wound.At present,
Domestic dressing industry kind is single, and functional shortcoming is lacked competitiveness in terms of skin repair wound compared with composite.
For Vaccarin and the feature of bacterial fibers and wound dressing exists at present problem, the present invention adopts
Prepare Vaccarin/bacterial cellulose composite wound dressing with unique combination process, protect playing Bacterial cellulose
While wet, permeation functions, utilize the feature of Vaccarin medicine, promote the healing of diabetic foot wound.
Summary of the invention
The invention discloses a kind of Vaccarin/Bacterial cellulose dressing treating diabetic foot ulcer, relate to
And a kind of medical apparatus and instruments and preparing technical field thereof.Preparation process of the present invention is easy to operate, low cost, the treatment diabetes of preparation
The moist aerogel dressing of ulcer of foot has the performances such as the damage of anti-high sugar, moisturizing, absorbing wound exudate and accelerating wound healing.
It is applicable to the wound repair such as diabetic foot ulcer, venous leg ulcers, pressure ulcer.
The invention discloses a kind of Vaccarin/Bacterial cellulose dressing treating diabetic foot ulcer.Bag
Include, Vaccarin medicine is dissolved in macromolecule polymer solution, obtains composite solution；Use infusion process, make antibacterial fine
Dimension element film immerses in Vaccarin/high molecular polymer composite solution；With UV-crosslinked, obtain Semen Vaccariae flavone
Glycosides/bacteria cellulose composite membrane, this dressing is the macromolecule polymer solution containing Vaccarin and Bacterial cellulose
Compound aquagel membrane.
A kind of Vaccarin/Bacterial cellulose dressing treating diabetic foot ulcer, described
Vaccarin is a kind of flavone three glucosides extracted from Semen Vaccariae, for faint yellow crystalline particulate, readily soluble
In methanol, methanol-water, ethanol, alcohol-water, n-butyl alcohol etc., it is insoluble in chloroform, ethyl acetate, petroleum ether etc., structure such as Fig. 1 institute
Show
Vaccarin can obviously improve cell viability, and experiment proves to wither the EA hy926 cell of high sugar induction
Die and there is obvious inhibitory action.Meanwhile, Vaccarin can promote the generation of endotheliocyte, has promotion angiogenesis
Effect.In use, by Vaccarin slow releasing function, can stimulate cellular proliferation and tissue repair, accelerate wound surface
Healing.
A kind of Vaccarin/Bacterial cellulose dressing treating diabetic foot ulcer, described
High molecular polymer is polyvinyl alcohol-styryl pyridine salt (PVA-SbQ), is a kind of photosensitive material.Wherein SbQ group is at purple
Outer light irradiates and issues third contact of a total solar or lunar eclipse dimerization reaction, forms cancellated polymer, and cladding medicine can be preferably fixed in photoinduction polymerization
Thing [Xiao Haijun .PVA-SbQ fixes chloroplast and the applied research [D] in detection herbicide thereof. Zhejiang University, and 2001.], with
Drug molecule forms stable structure.Meanwhile, PVA-SbQ has good biocompatibility, can apply to wound dressing neck
Territory.
A kind of Vaccarin/Bacterial cellulose dressing treating diabetic foot ulcer, described
The solvent of macromolecule polymer solution is deionized water.
A kind of Vaccarin/Bacterial cellulose dressing treating diabetic foot ulcer, described
Macromolecule polymer solution containing Vaccarin, can be by controlling the required flavone of concentration preparation of Vaccarin
The composite solution of glycosides content.
A kind of Vaccarin/Bacterial cellulose dressing treating diabetic foot ulcer, described
The preparation of bacteria cellulose film is under aseptic processing environment, is received by acetobacter xylinum in seed culture medium, is put on shaking table training
Support 3 days.Seed culture medium after being cultivated by shaking table is received in fermentation medium, is then put in static culture case cultivation 7 days.Take
Go out Bacterial cellulose water bath processing 2h in 4%NaOH solution, then with deionized water rinsing Bacterial cellulose until PH is 7.
Prepared bacteria cellulose film has natural three-dimensional manometer network structure, can make antibiotic substance, medicine, small-molecule substance
Wound surface is entered into Deng through dressing.
A kind of Vaccarin/Bacterial cellulose dressing treating diabetic foot ulcer, described
Using infusion process to make bacteria cellulose film immerse in Vaccarin/macromolecule polymer solution, dip time is 30min
～2h.
A kind of Vaccarin/Bacterial cellulose dressing treating diabetic foot ulcer, described
Using cross-linking method, be to irradiate under the uviol lamp of 400W, irradiation time is 0.5～2h, obtains Vaccarin/antibacterial fine
Dimension element composite membrane.Then by Vaccarin/bacteria cellulose film of having cross-linked through normal temperature drying or lyophilization,
To aerogel dressing.
Compared with prior art, the invention has the beneficial effects as follows:
This patent is respectively adopted infusion process and cross-linking method prepares a kind of Semen Vaccariae flavone treating diabetic foot ulcer
Glycosides/Bacterial cellulose dressing, this dressing is the compound water congealing glued membrane of bacteria cellulose aquagel and Vaccarin composition.
Feature and the anti-high sugar of Vaccarin of having gathered bacteria cellulose aquagel dressing damage and the medicine promoting angiogenesis
Effect feature and the moist anti-bacterial hydrogel dressing prepared, have great clinical value for treatment diabetic foot ulcer.
Preparation process of the present invention is easy to operate, low cost, the moist aerogel dressing of the treatment diabetic foot ulcer of preparation
There is the performances such as the damage of anti-high sugar, moisturizing, absorbing wound exudate and accelerating wound healing.
Accompanying drawing explanation
Fig. 1 is the molecular structure of Vaccarin in the present invention.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is expanded on further.Should be understood that these embodiments are merely to illustrate the present invention
Rather than restriction the scope of the present invention.In addition, it is to be understood that after having read the content that the present invention lectures, people in the art
The present invention can be made various changes or modifications by member, and these equivalent form of values fall within the application appended claims equally and limited
Scope.
Embodiment 1:
1, during 3gPVA-SbQ is dissolved in 30mL deionized water, 2.0mg flavonoid glycoside is joined in PVA-SbQ solution, obtains
PVA-SbQ/ flavonoid glycoside solution.
2, bacteria cellulose film by acetobacter xylinum output and separated, that purify is immersed in PVA-SbQ solution 24 little
Time, irradiate through 400W ultraviolet light and obtain Vaccarin/bacteria cellulose composite membrane in 1 hour.Through normal temperature drying, obtain
A kind of moist anti-bacterial hydrogel dressing treating diabetic foot ulcer, this dressing be bacteria cellulose aquagel with containing king
Do not stay the compound water congealing glued membrane of row flavonoid glycoside PVA-SbQ solution composition.
Embodiment 2:
1, during 3gPVA-SbQ is dissolved in 30mL deionized water, 1.8mg flavonoid glycoside is joined in PVA-SbQ solution, obtains
PVA-SbQ/ flavonoid glycoside solution.
2, bacteria cellulose film by acetobacter xylinum output and separated, that purify is immersed in PVA-SbQ solution 24 little
Time, irradiate through 400W ultraviolet light and obtain Vaccarin/bacteria cellulose composite membrane in 1 hour.Through normal temperature drying, obtain
A kind of moist anti-bacterial hydrogel dressing treating diabetic foot ulcer, this dressing be bacteria cellulose aquagel with containing king not
Stay the compound water congealing glued membrane of row flavonoid glycoside PVA-SbQ solution composition.
Embodiment 3:
1, during 3gPVA-SbQ is dissolved in 30mL deionized water, 1.6mg flavonoid glycoside is joined in PVA-SbQ solution, obtains
PVA-SbQ/ flavonoid glycoside solution.
2, bacteria cellulose film by acetobacter xylinum output and separated, that purify is immersed in PVA-SbQ solution 24 little
Time, irradiate 1 hour through 400W ultraviolet light, obtain Vaccarin/bacteria cellulose composite membrane.Through normal temperature drying,
To a kind of moist anti-bacterial hydrogel dressing treating diabetic foot ulcer, this dressing be bacteria cellulose aquagel with containing king
Do not stay the compound water congealing glued membrane of row flavonoid glycoside PVA-SbQ solution composition.
Embodiment 4:
1, during 3gPVA-SbQ is dissolved in 30mL deionized water, 2.0mg flavonoid glycoside is joined in PVA-SbQ solution, obtains
PVA-SbQ/ flavonoid glycoside solution.(deionized water that solvent is all)
2, bacteria cellulose film by acetobacter xylinum output and separated, that purify is immersed in PVA-SbQ solution 24 little
Time, irradiate half an hour through 400W ultraviolet light, obtain Vaccarin/bacteria cellulose composite membrane.Through lyophilization,
To a kind of moist anti-bacterial hydrogel dressing treating diabetic foot ulcer, this dressing be bacteria cellulose aquagel with containing king
Do not stay the compound water congealing glued membrane of row flavonoid glycoside PVA-SbQ solution composition.
Embodiment 5:
1, during 3gPVA-SbQ is dissolved in 30mL deionized water, 2.0mg flavonoid glycoside is joined in PVA-SbQ solution, obtains
PVA-SbQ/ flavonoid glycoside solution.
2, bacteria cellulose film by acetobacter xylinum output and separated, that purify is immersed in PVA-SbQ solution 24 little
Time, irradiate or 2 hours through 400W ultraviolet light, obtain Vaccarin/bacteria cellulose composite membrane.Through lyophilization,
Obtaining a kind of moist anti-bacterial hydrogel dressing treating diabetic foot ulcer, this dressing is bacteria cellulose aquagel and contain
The compound water congealing glued membrane of Vaccarin PVA-SbQ solution composition.
Claims (4)
1. treat Vaccarin/Bacterial cellulose dressing of diabetic foot ulcer, it is characterized in that: by Semen Vaccariae
Flavonoid glycoside medicine is dissolved in macromolecule polymer solution, obtains composite solution；Use infusion process, make bacteria cellulose film immerse king not
Stay in row flavonoid glycoside/high molecular polymer composite solution；With UV-crosslinked, obtain Vaccarin/Bacterial cellulose multiple
Closing film, this dressing is the aquagel membrane that the macromolecule polymer solution containing Vaccarin is compound with Bacterial cellulose,
The described macromolecule polymer solution containing Vaccarin, it is possible to prepared by the concentration controlling Vaccarin
The composite solution of required flavonoid glycoside content, wherein the solvent of macromolecule polymer solution is deionized water；
Described high molecular polymer is polyvinyl alcohol-styryl pyridine salt (PVA-SbQ), is a kind of photosensitive material；
Described is UV-crosslinked, irradiates under the uviol lamp of 400W, and irradiation time is 0.5～2h.
A kind of Vaccarin/Bacterial cellulose dressing treating diabetic foot ulcer, its feature
It is: described Vaccarin is a kind of flavone three glucosides extracted from Semen Vaccariae, for faint yellow graininess
Crystallization.
A kind of Vaccarin/Bacterial cellulose dressing treating diabetic foot ulcer, its feature
Be: described bacteria cellulose film is under aseptic processing environment, by acetobacter xylinum output, through 4%NaOH solution and go from
Sub-water processes.
A kind of Vaccarin/Bacterial cellulose dressing treating diabetic foot ulcer, its feature
It is: described employing infusion process makes bacteria cellulose film immerse in Vaccarin/macromolecule polymer solution, during dipping
Between be 30min～2h.
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| CN201410648771.1A CN104436291B (en) | 2014-11-17 | 2014-11-17 | A kind of Vaccarin/Bacterial cellulose dressing treating diabetic foot ulcer |
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| CN201410648771.1A CN104436291B (en) | 2014-11-17 | 2014-11-17 | A kind of Vaccarin/Bacterial cellulose dressing treating diabetic foot ulcer |
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| CN104436291B true CN104436291B (en) | 2016-08-17 |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105168234A (en) * | 2015-09-06 | 2015-12-23 | 江南大学 | Application of vaccarin in treating open skin lesions |
| CN106727282A (en) * | 2016-12-20 | 2017-05-31 | 清华大学深圳研究生院 | A kind of gel preparation for preventing and treating diabetes and preparation method thereof |
| CN108853558B (en) * | 2018-05-25 | 2021-07-16 | 中山大学 | Microbial cellulose composite dressing with RNA interference function and preparation method and application thereof |
| CN113577096B (en) * | 2021-08-16 | 2022-08-09 | 江南大学 | Combined preparation for treating chronic wounds and preparation method thereof |
| CN113842492A (en) * | 2021-09-23 | 2021-12-28 | 江南大学 | Hydrogel/non-woven material composite dressing loaded with centella asiatica extract |
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| CN102212208A (en) * | 2010-04-07 | 2011-10-12 | 南京理工大学 | Preparation method of bacteria cellulose/hyaluronic acid composite |
| CN103041438A (en) * | 2012-12-31 | 2013-04-17 | 钟春燕 | Moist antibacterial hydrogel dressing for curing diabetic foot ulcer |
| CN103948619A (en) * | 2014-05-12 | 2014-07-30 | 江南大学 | Application of vaccarin for resisting oxidation and high-glucose damage |
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| PL212003B1 (en) * | 2003-07-03 | 2012-07-31 | Politechnika Łodzka | Method for obtaining bacterial cellulose, method for bacteria immobilization, method for obtaining immobilized biocatalysts, application of bacterial cellulose, method for modification of cellulose membranes |
| CA2669438C (en) * | 2006-04-24 | 2015-06-16 | Axcelon Biopolymers Corporation | Nanosilver coated bacterial cellulose |
| DE202011108802U1 (en) * | 2011-09-02 | 2012-09-03 | BLüCHER GMBH | Wound dressing with antimicrobial effect |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1766102A (en) * | 2005-08-31 | 2006-05-03 | 农业部环境保护科研监测所 | PVA-SbQ synthesis and acetylcholinesterase fixing by same |
| CN102212208A (en) * | 2010-04-07 | 2011-10-12 | 南京理工大学 | Preparation method of bacteria cellulose/hyaluronic acid composite |
| CN103041438A (en) * | 2012-12-31 | 2013-04-17 | 钟春燕 | Moist antibacterial hydrogel dressing for curing diabetic foot ulcer |
| CN103948619A (en) * | 2014-05-12 | 2014-07-30 | 江南大学 | Application of vaccarin for resisting oxidation and high-glucose damage |
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