CN113842492A - Hydrogel/non-woven material composite dressing loaded with centella asiatica extract - Google Patents
Hydrogel/non-woven material composite dressing loaded with centella asiatica extract Download PDFInfo
- Publication number
- CN113842492A CN113842492A CN202111125588.XA CN202111125588A CN113842492A CN 113842492 A CN113842492 A CN 113842492A CN 202111125588 A CN202111125588 A CN 202111125588A CN 113842492 A CN113842492 A CN 113842492A
- Authority
- CN
- China
- Prior art keywords
- hydrogel
- extract
- centella asiatica
- loaded
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/40—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing ingredients of undetermined constitution or reaction products thereof, e.g. plant or animal extracts
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/26—Macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/28—Polysaccharides or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/32—Proteins, polypeptides; Degradation products or derivatives thereof, e.g. albumin, collagen, fibrin, gelatin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/46—Deodorants or malodour counteractants, e.g. to inhibit the formation of ammonia or bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/62—Compostable, hydrosoluble or hydrodegradable materials
-
- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M13/00—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with non-macromolecular organic compounds; Such treatment combined with mechanical treatment
-
- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M13/00—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with non-macromolecular organic compounds; Such treatment combined with mechanical treatment
- D06M13/10—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with non-macromolecular organic compounds; Such treatment combined with mechanical treatment with compounds containing oxygen
- D06M13/184—Carboxylic acids; Anhydrides, halides or salts thereof
- D06M13/207—Substituted carboxylic acids, e.g. by hydroxy or keto groups; Anhydrides, halides or salts thereof
-
- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M15/00—Treating fibres, threads, yarns, fabrics, or fibrous goods made from such materials, with macromolecular compounds; Such treatment combined with mechanical treatment
- D06M15/01—Treating fibres, threads, yarns, fabrics, or fibrous goods made from such materials, with macromolecular compounds; Such treatment combined with mechanical treatment with natural macromolecular compounds or derivatives thereof
- D06M15/03—Polysaccharides or derivatives thereof
-
- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M15/00—Treating fibres, threads, yarns, fabrics, or fibrous goods made from such materials, with macromolecular compounds; Such treatment combined with mechanical treatment
- D06M15/01—Treating fibres, threads, yarns, fabrics, or fibrous goods made from such materials, with macromolecular compounds; Such treatment combined with mechanical treatment with natural macromolecular compounds or derivatives thereof
- D06M15/15—Proteins or derivatives thereof
-
- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M16/00—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/30—Compounds of undetermined constitution extracted from natural sources, e.g. Aloe Vera
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
-
- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M2101/00—Chemical constitution of the fibres, threads, yarns, fabrics or fibrous goods made from such materials, to be treated
- D06M2101/16—Synthetic fibres, other than mineral fibres
- D06M2101/30—Synthetic polymers consisting of macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
- D06M2101/32—Polyesters
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Epidemiology (AREA)
- Materials Engineering (AREA)
- Animal Behavior & Ethology (AREA)
- Hematology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Textile Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Botany (AREA)
- Zoology (AREA)
- Medicinal Preparation (AREA)
Abstract
The invention discloses a hydrogel/non-woven material composite dressing loaded with a centella asiatica extract, and belongs to the field of medical and health materials. The method for preparing the composite dressing comprises the following steps: (1) adding acetic acid, gelatin and chitosan into the centella extract solution, and uniformly mixing to obtain a hydrogel precursor solution; (2) adding a hydrogel precursor solution into a container, then covering a layer of non-woven material, continuously adding the hydrogel precursor solution, and performing crosslinking to obtain a hydrogel/non-woven material composite material loaded with the centella asiatica extract; (3) and (3) soaking the hydrogel/non-woven material composite material loaded with the asiatic pennywort herb extract in a trisodium citrate solution, and performing crosslinking again to obtain the hydrogel/non-woven material composite dressing loaded with the asiatic pennywort herb extract. The breaking strength of the composite dressing is up to 0.86MPa, the breaking elongation is more than 70%, and the diameter of a bacteriostatic circle for staphylococcus aureus is more than 4.9 mm.
Description
Technical Field
The invention relates to a hydrogel/non-woven material composite dressing loaded with a centella asiatica extract, belonging to the field of medical and health materials.
Background
The hydrogel is a polymer with a three-dimensional network structure, has strong water absorption and retention capacity, and is soft and comfortable in hand feeling. The hydrogel dressing can absorb wound exudate, does not adhere to a wound, does not cause secondary damage during dressing change, can provide a moist growth environment for the wound, effectively prevents invasion of microorganisms such as bacteria and fungi, is beneficial to rapid growth of epithelial cells, promotes rapid healing of the wound, and is an excellent dressing material.
Centella asiatica is a dry whole plant of an Umbelliferae plant, is a creeping herb for many years, is also called as water chestnut, Chinese lizardtail, longhairy antenoron herb, old male root and the like, is widely distributed on roadside and field in the south of Yangtze river, has slender stem, round leaves or kidney-shaped leaves and sawteeth at the edge, and is used as a folk common Chinese herbal medicine for thousands of years. The herba Centellae contains asiaticoside as main active component, and has antiulcer, antiinflammatory, antibacterial, antidepressant, dementia relieving, tranquilizing, wound healing promoting, and scar growth inhibiting effects.
At present, centella asiatica extract is used for preparing gel dressings, for example, Chinese patent CN112316205A discloses a preparation method of asiaticoside gel dressings, the gel dressings are used as carriers for external administration, secondary injury caused by dressing change is reduced, and the asiaticoside gel dressings have certain effectiveness and safety on wound surfaces. Chinese patent CN106075539A discloses a preparation method of a asiaticoside-loaded nanofiber membrane with a core/shell structure, which takes polylactic acid/glycollic acid copolymer with biocompatibility and biodegradability as a shell and Polycaprolactone (PLA) loaded asiaticoside as an inner core, and adopts coaxial electrostatic spinning to prepare the drug-loaded nanofiber membrane with the core/shell structure, thereby having excellent advantage in the aspect of promoting wound healing. In addition, the invention patents of CN107669757A, CN108653129A, skin dressing containing natural plant powerful anti-allergy composition, CN108567638A, powerful moisturizing dressing with epidermis repair function, CN105497960A, medical dressing with wound healing promoting function and preparation method, CN110711263A, functional dressing with scar preventing, repairing and modifying functions and preparation method thereof, CN111166929A, moist wound dressing and preparation method thereof, etc. all introduce centella asiatica and its extract to prepare dressing to promote wound healing and prevent scar growth. However, these hydrogels have a good wound healing promoting effect, but are slightly deficient in mechanical properties.
Disclosure of Invention
[ problem ] to
The existing medical dressing has poor antibacterial performance and low mechanical strength, and is easy to cause secondary damage due to wound adhesion during dressing change.
[ solution ]
In order to solve at least one problem, the invention mixes the natural high molecular polymer with biodegradability to prepare the hydrogel wound dressing which is safe, nontoxic and good in biocompatibility, simultaneously adds the non-woven material to play a role in enhancing the mechanics, overcomes the defect of low mechanical strength of the traditional hydrogel dressing, and introduces the traditional Chinese medicine centella to improve the antibacterial performance of the material. The hydrogel/non-woven material composite dressing prepared by the invention has strong water absorption and moisture retention, provides cool touch and moist environment for wounds, has good antibacterial performance, can effectively prevent bacterial invasion, and promotes wound healing.
The first purpose of the invention is to provide a method for preparing a centella asiatica extract-loaded hydrogel/nonwoven composite dressing, which comprises the following steps:
(1) adding acetic acid, gelatin and chitosan into the centella extract solution, and uniformly mixing to obtain a hydrogel precursor solution;
(2) adding the hydrogel precursor solution obtained in the step (1) into a container, then covering a layer of non-woven material, continuously adding the hydrogel precursor solution, and performing crosslinking to obtain a hydrogel/non-woven material composite material loaded with the centella asiatica extract;
(3) and (3) soaking the hydrogel/non-woven material composite material loaded with the asiatic pennywort herb extract obtained in the step (2) in a trisodium citrate solution, and performing crosslinking again to obtain the hydrogel/non-woven material composite dressing loaded with the asiatic pennywort herb extract.
In one embodiment of the present invention, the centella asiatica extract in step (1) is obtained by crushing dried centella asiatica into powder, condensing and refluxing with ethanol solution, filtering, and freeze-drying; wherein the particle size of the centella powder is 80-120 nm, the ethanol solution is an ethanol water solution, and the concentration is 70-80% v/v; the condensing reflux temperature is 60-70 ℃, and the time is 5-6 h; the freeze drying temperature is-40 ℃ to-80 ℃ and the time is 24h to 48 h; the concentration of the centella asiatica powder in the ethanol solution is 0.1 g/mL-0.2 g/mL.
In one embodiment of the present invention, the solvent of the centella asiatica extract solution in step (1) is water, and the concentration is 0.001g/mL to 0.003 g/mL.
In one embodiment of the present invention, the concentration of acetic acid in the centella asiatica extract solution in step (1) is 1% to 2% v/v.
In one embodiment of the present invention, the ratio of the centella asiatica extract, the gelatin and the chitosan in the centella asiatica extract solution in the step (1) is 0.05-0.15: 10: 1 to 3.
In one embodiment of the invention, the step (1) of uniformly mixing is performed by stirring at 50-60 ℃ for 50-70 min.
In one embodiment of the present invention, the nonwoven material of step (2) comprises polylactic acid (PLA) hot air nonwoven material, polylactic acid spunbond nonwoven material, polylactic acid thermo-bonded nonwoven material; before use, the front and back sides are subjected to ultraviolet irradiation for 30min for sterilization treatment; the gram weight is 20-30 g/m2。
In one embodiment of the invention, the thickness of the hydrogel precursors of the upper layer and the lower layer of the non-woven material in the step (2) is 0.5-1 mm.
In one embodiment of the present invention, the crosslinking in step (2) is performed at 20-30 ℃ (room temperature) for 20-30 h.
In one embodiment of the present invention, the concentration of the trisodium citrate aqueous solution in the step (3) is 15 to 25 wt%, the dipping time is 1 to 3 days, and the dipping temperature is 20 to 30 ℃ (room temperature).
The second purpose of the invention is the hydrogel/non-woven material composite dressing loaded with the asiatic pennywort herb extract prepared by the method.
The third purpose of the invention is the application of the asiatic pennywort herb extract-loaded hydrogel/non-woven material composite dressing in the field of medical and health materials.
The fourth purpose of the invention is to provide a bandage which adopts the hydrogel/non-woven material composite dressing loaded with the asiatic pennywort herb extract.
[ advantageous effects ]
(1) The invention adopts the gelatin and the chitosan as biodegradable raw materials, and the hydrogel prepared from the gelatin and the chitosan has good biocompatibility and biodegradability, is harmless to human bodies and has strong absorption capacity to wound exudate.
(2) The centella extract adopted by the invention belongs to traditional Chinese medicine components with the effects of resisting bacteria, diminishing inflammation, resisting ulcer and inhibiting scar growth, and is beneficial to improving the antibacterial property of the wound dressing and accelerating the healing speed of the wound.
(3) The obtained hydrogel/non-woven material composite dressing loaded with the centella asiatica extract has a sandwich structure, and the non-woven material interlayer in the middle is used as a supporting layer, so that the mechanical property of the dressing is enhanced, and the defect of low mechanical strength is overcome; the hydrogel of the upper and lower layers carries herba Centellae extract, and has antibacterial, antiinflammatory, and wound healing promoting effects.
(4) The preparation method is simple, the dressing has high water content, good water absorption, good antibacterial performance and mechanical strength, provides a moist environment for the wound and is beneficial to the healing of the wound; the dressing is soft and comfortable, has good application performance, and is suitable for wound care of various parts of a human body.
(5) The breaking strength of the obtained hydrogel/non-woven material composite dressing loaded with the centella asiatica extract is up to 0.86MPa, the breaking elongation is more than 70%, and the diameter of a bacteriostatic circle for staphylococcus aureus is more than 4.9 mm.
Drawings
FIG. 1 is a flow chart of the preparation process of example 1.
Fig. 2 is a topographical view of the centella asiatica extract-loaded hydrogel/PLA nonwoven composite dressing of example 1.
Detailed Description
The following description of the preferred embodiments of the present invention is provided for the purpose of better illustrating the invention and is not intended to limit the invention thereto.
The test method comprises the following steps:
testing of tensile Strength: the Test was carried out on an EZ-Test series single column electronic universal tester (EZ-LX, Shimadzu, Japan). Cutting the prepared sheet sample into strips of 0.5cm multiplied by 10cm, measuring and recording the thickness of the sample by a micrometer, setting the sample holding distance to be 50mm, setting the stretching speed to be 5mm/min, testing each group of samples for 10 times and calculating the average value.
And (3) testing antibacterial property: GB/T20944.1-2007 evaluation part 1 of antibacterial performance of textiles: the antibacterial performance of the sample by the bacteriostatic circle method of agar plate diffusion method is tested, and the antibacterial effect of the sample on staphylococcus aureus (S.aureus, ATCC6538) is evaluated. Inoculating the two bacteria into nutrient broth, culturing at 37 + -2 deg.C for 18-24 hr, diluting with distilled water 20 times, and adjusting the concentration of bacteria liquid to 1 × 106CFU/mL～5×106CFU/mL was used as the test bacterial liquid. 10mL of agar medium was poured into a sterile plate and allowed to clot. 150mL of agar medium with the temperature of 45 ℃ is taken and placed into a flask, 1mL of test bacterium liquid is added, and the flask is shaken up and then injected into a congealed plate for 5mL of congelation. Cutting the sample to be tested into a circle with a diameter of 15mm, placing the circle in the middle of the plate, and finally placing the circle in an incubator at 37 +/-2 ℃ for culturing for 18h. The antibacterial effect of the sample was evaluated by measuring the width of the zone.
The specific parameters of the substances employed in the examples: chitosan (CS, the degree of deacetylation is 80-95%, the viscosity is 50-800 mPa · s), gelatin (Gel, the viscosity is more than or equal to 15.0 mm)2(s), acetic Acid (AR), trisodium citrate, ethanol, etc. are available from the national pharmaceutical group chemical agents, Inc.; polylactic acid (PLA) nonwoven materials (NF) were purchased from Hengtian Yangtze biomaterial, Inc. and had a grammage of about 20g/m2。
Example 1
A method of making a snowmobile-loaded hydrogel/PLA nonwoven composite dressing, comprising the steps of:
(1) crushing dried herba Centellae into powder (with particle size of 100 nm); weighing 20g of herba Centellae powder, adding into 100mL of 70% ethanol water solution, heating in water bath at 60 deg.C, condensing, refluxing for 5h, filtering, and freezing the filtrate in a freeze-drying machine at-40 deg.C for 24h to obtain herba Centellae extract;
(2) weighing 0.1g of centella asiatica extract, and dissolving in 50mL of water to obtain a centella asiatica extract solution; then adding 500 mu L of acetic acid, 10g of gelatin and 2g of chitosan into the centella asiatica extract solution, heating and stirring in a water bath at 60 ℃ for 60min to obtain hydrogel precursor solution;
(3) cutting the PLA non-woven material into small blocks of 10cm multiplied by 10cm, sterilizing the small blocks under an ultraviolet lamp, and irradiating the front side and the back side of the small blocks for 30min respectively; injecting the hydrogel precursor solution prepared in the step (2) into a square (10cm multiplied by 10cm) sterile culture dish, then paving the sterilized PLA non-woven material in the culture dish, continuously injecting the hydrogel precursor solution, wherein the thickness of the hydrogel precursor at the upper layer and the lower layer is 0.5mm, and then placing the hydrogel precursor at 25 ℃ (room temperature) for crosslinking for 24 hours to obtain the hydrogel/PLA non-woven composite material loaded with the centella asiatica extract;
(4) and (4) soaking the hydrogel/PLA non-woven material composite material loaded with the asiatic pennywort herb extract prepared in the step (3) in a 20% trisodium citrate aqueous solution at 25 ℃ (room temperature) for 3 days, and performing crosslinking again to obtain the hydrogel/PLA non-woven material composite dressing loaded with the asiatic pennywort herb extract.
Comparative example 1
Step (4) in example 1 was omitted, and the rest was in accordance with example 1, to obtain a hydrogel/PLA nonwoven material composite dressing loaded with centella asiatica extract.
Comparative example 2
The PLA nonwoven material of example 1 was omitted and the rest was identical to example 1, resulting in a snowflake extract-loaded hydrogel dressing.
Comparative example 3
The centella asiatica extract was omitted from example 1, and the procedure was otherwise identical to example 1, to obtain a hydrogel/PLA nonwoven composite dressing free of centella asiatica extract.
Comparative example 4
The procedure (1) and the procedure (4) in example 1 were omitted, and the snowflake extract and the nonwoven material were not added and were not soaked in the trisodium citrate solution, but the procedure was otherwise identical to example 1, in which 500. mu.L of acetic acid was added to 50mL of water, 10g of gelatin and 2g of chitosan were added to the aqueous solution of acetic acid, the mixture was heated in a water bath at 60 ℃ for 60min and stirred uniformly to obtain a hydrogel precursor solution, and then a square (10 cm. times.10 cm) sterile petri dish was poured to obtain a hydrogel dressing.
The composite dressings obtained in example 1 and comparative examples 1 to 4 were tested, and the test results were as follows:
TABLE 1 test results of example 1 and comparative examples 1 to 4
As can be seen from table 1: the hydrogel/PLA non-woven material composite dressing loaded with the centella asiatica extract in example 1 has good mechanical property and antibacterial property. Compared with the comparative examples 1-4 and the example 1, the addition of the non-woven material can play a role in enhancing, so that the breaking strength of the composite hydrogel dressing is increased; the addition of the centella extract improves the antibacterial activity of the hydrogel composite dressing, and increases the antibacterial zone around the composite hydrogel dressing in the culture process.
Example 2 optimization of the mass ratio of gelatin to chitosan
Composite dressings were obtained by adjusting the amount of chitosan in example 1 to 1 and 3g, and keeping the same as in example 1.
The obtained composite dressing was tested, and the test results were as follows:
table 2 test results of example 2
Example 3 amount of centella asiatica extract
The amount of centella asiatica extract in example 1 was adjusted to 0.05, 0.15g, 0.5g, 1g, 1.5g, and the other amounts were the same as in example 1, to obtain a composite dressing.
The obtained composite dressing was tested, and the test results were as follows:
table 3 test results of example 3
Although the present invention has been described with reference to the preferred embodiments, it should be understood that various changes and modifications can be made therein by those skilled in the art without departing from the spirit and scope of the invention as defined in the appended claims.
Claims (10)
1. A method for preparing a snowflake extract-loaded hydrogel/nonwoven composite dressing, comprising the steps of:
(1) adding acetic acid, gelatin and chitosan into the centella extract solution, and uniformly mixing to obtain a hydrogel precursor solution;
(2) adding the hydrogel precursor solution obtained in the step (1) into a container, then covering a layer of non-woven material, continuously adding the hydrogel precursor solution, and performing crosslinking to obtain a hydrogel/non-woven material composite material loaded with the centella asiatica extract;
(3) and (3) soaking the hydrogel/non-woven material composite material loaded with the asiatic pennywort herb extract obtained in the step (2) in a trisodium citrate solution, and performing crosslinking again to obtain the hydrogel/non-woven material composite dressing loaded with the asiatic pennywort herb extract.
2. The method according to claim 1, wherein the ratio of centella asiatica extract, gelatin and chitosan in the centella asiatica extract solution of step (1) is 0.05-0.15: 10: 1 to 3.
3. The method according to claim 1 or 2, wherein the extract of centella asiatica of step (1) is obtained by crushing dried centella asiatica into powder, condensing and refluxing with ethanol solution, filtering, and freeze-drying; wherein the particle size of the centella powder is 80-120 nm, the ethanol solution is an ethanol water solution, and the concentration is 70-80% v/v; the condensing reflux temperature is 60-70 ℃, and the time is 5-6 h; the freeze drying temperature is-40 ℃ to-80 ℃ and the time is 24h to 48 h; the concentration of the centella asiatica powder in the ethanol solution is 0.1 g/mL-0.2 g/mL.
4. The method according to any one of claims 1 to 3, wherein the solvent of the centella asiatica extract solution in step (1) is water at a concentration of 0.001g/mL to 0.003 g/mL.
5. The method according to any one of claims 1 to 4, wherein the thickness of the hydrogel precursor of the upper and lower layers of the nonwoven material in step (2) is 0.5 to 1 mm.
6. The method as claimed in any one of claims 1 to 5, wherein the concentration of the aqueous solution of trisodium citrate in step (3) is 15 to 25 wt%, the dipping time is 1 to 3 days, and the dipping temperature is 20 to 30 ℃.
7. The method according to any one of claims 1 to 6, wherein the nonwoven material of step (2) comprises a polylactic acid (PLA) hot air nonwoven materialPolylactic acid spun-bonded nonwoven materials, polylactic acid hot-rolled nonwoven materials; the gram weight is 20-30 g/m2。
8. The centella asiatica extract-loaded hydrogel/nonwoven composite dressing prepared by the method according to any one of claims 1 to 7.
9. Use of the centella asiatica extract-loaded hydrogel/nonwoven composite dressing according to claim 8 in the field of medical and health materials.
10. A wound covering, characterized in that it uses the asiatic pennywort herb extract-loaded hydrogel/nonwoven material composite dressing of claim 8.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111125588.XA CN113842492A (en) | 2021-09-23 | 2021-09-23 | Hydrogel/non-woven material composite dressing loaded with centella asiatica extract |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111125588.XA CN113842492A (en) | 2021-09-23 | 2021-09-23 | Hydrogel/non-woven material composite dressing loaded with centella asiatica extract |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113842492A true CN113842492A (en) | 2021-12-28 |
Family
ID=78979947
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111125588.XA Pending CN113842492A (en) | 2021-09-23 | 2021-09-23 | Hydrogel/non-woven material composite dressing loaded with centella asiatica extract |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113842492A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114306716A (en) * | 2022-01-24 | 2022-04-12 | 苏州大学 | Preparation method of temperature-sensitive gel type medical dressing based on fabric |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100239621A1 (en) * | 2007-10-31 | 2010-09-23 | Fujifilm Corporation | Gel sheet and cosmetic preparation in sheet form using the same |
| KR20110057563A (en) * | 2009-11-24 | 2011-06-01 | 한국원자력연구원 | Hydrogels for wound dressings comprising beta-glucan and preparation method thereof |
| CN104436291A (en) * | 2014-11-17 | 2015-03-25 | 江南大学 | Cowherb seed flavonoid glycoside/bacterial cellulose dressing for treating diabetic foot ulcers and preparation method thereof |
| CN108686252A (en) * | 2018-06-11 | 2018-10-23 | 南方医科大学 | It is a kind of using chitosan-poloxamer as nano silver antibiotic dressing of matrix and its preparation method and application |
| CN110507845A (en) * | 2019-09-25 | 2019-11-29 | 广州沁瀚生物科技有限公司 | Biological composite ventilating dressing and preparation method thereof |
| CN112316205A (en) * | 2020-11-16 | 2021-02-05 | 中国药科大学 | External asiaticoside gel dressing and preparation method thereof |
| CN112717194A (en) * | 2020-12-30 | 2021-04-30 | 河南亚都实业有限公司 | Degradable chitosan-based composite hemostatic film |
| CN113041391A (en) * | 2021-03-31 | 2021-06-29 | 广州源肽生物科技有限公司 | Herba centellae-collagen-containing repairing freeze-dried sponge and preparation method thereof |
-
2021
- 2021-09-23 CN CN202111125588.XA patent/CN113842492A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100239621A1 (en) * | 2007-10-31 | 2010-09-23 | Fujifilm Corporation | Gel sheet and cosmetic preparation in sheet form using the same |
| KR20110057563A (en) * | 2009-11-24 | 2011-06-01 | 한국원자력연구원 | Hydrogels for wound dressings comprising beta-glucan and preparation method thereof |
| CN104436291A (en) * | 2014-11-17 | 2015-03-25 | 江南大学 | Cowherb seed flavonoid glycoside/bacterial cellulose dressing for treating diabetic foot ulcers and preparation method thereof |
| CN108686252A (en) * | 2018-06-11 | 2018-10-23 | 南方医科大学 | It is a kind of using chitosan-poloxamer as nano silver antibiotic dressing of matrix and its preparation method and application |
| CN110507845A (en) * | 2019-09-25 | 2019-11-29 | 广州沁瀚生物科技有限公司 | Biological composite ventilating dressing and preparation method thereof |
| CN112316205A (en) * | 2020-11-16 | 2021-02-05 | 中国药科大学 | External asiaticoside gel dressing and preparation method thereof |
| CN112717194A (en) * | 2020-12-30 | 2021-04-30 | 河南亚都实业有限公司 | Degradable chitosan-based composite hemostatic film |
| CN113041391A (en) * | 2021-03-31 | 2021-06-29 | 广州源肽生物科技有限公司 | Herba centellae-collagen-containing repairing freeze-dried sponge and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| DAWEI LI ET AL.: "Mechanically-reinforced 3D scaffold constructed by silk nonwoven fabric and silk fibroin sponge", 《COLLOIDS AND SURFACES B: BIOINTERFACES》 * |
| 王晓波: "《药物运释系统》", 31 August 2007, 中国医药科技出版社 * |
| 黄治林等: "透明型明胶／壳聚糖烧伤创面敷料的研制", 《中华烧伤杂志》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114306716A (en) * | 2022-01-24 | 2022-04-12 | 苏州大学 | Preparation method of temperature-sensitive gel type medical dressing based on fabric |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Asghari et al. | Hybrid PCL/chitosan-PEO nanofibrous scaffolds incorporated with A. euchroma extract for skin tissue engineering application | |
| CN102258801B (en) | Sponge calcium alginate medical dressing, and preparation method | |
| WO2019091150A1 (en) | Alginate wound repair dressing and preparation method thereof | |
| He et al. | Bacterial cellulose: functional modification and wound healing applications | |
| Rao et al. | Fungal-derived carboxymethyl chitosan blended with polyvinyl alcohol as membranes for wound dressings | |
| CN104623718B (en) | Chitosan petrolatum gauze and preparation method thereof | |
| CN103340722A (en) | Sanitary towel good in water-absorbing performance | |
| Sadeghi-Aghbash et al. | Fabrication and development of PVA/Alginate nanofibrous mats containing Arnebia Euchroma extract as a burn wound dressing | |
| CN103341202B (en) | Chitosan sponge surgical dressing and preparation method thereof | |
| CN100540065C (en) | The manufacture method of cross-linking radiation holey human acellular amniotic membrane aerogel dressing | |
| CN106110383A (en) | A kind of chitosan alginate dressing and freeze-drying process thereof | |
| Balusamy et al. | Electrospun nanofibrous materials for wound healing applications | |
| CN110507842B (en) | Bacterial cellulose/hyaluronic acid/epsilon-polylysine functional dressing and preparation method thereof | |
| CN104474573B (en) | Biological mask and preparation method thereof | |
| WO2007091801A1 (en) | A sheet device comprising bio-cellulose for alleviating skin damage and relieving skin problem | |
| CN115154642A (en) | Bionic asymmetric sponge dressing and preparation method thereof | |
| CN105288698A (en) | Calcium alginate composite medical surgical dressing and preparation method thereof | |
| CN107823699A (en) | Bleeding stopping and adherence preventing film and preparation method thereof | |
| CN113842492A (en) | Hydrogel/non-woven material composite dressing loaded with centella asiatica extract | |
| Li et al. | Sandwich structure Aloin-PVP/Aloin-PVP-PLA/PLA as a wound dressing to accelerate wound healing | |
| KR101212504B1 (en) | Antimicrobial hydrogel comprising coptis chinesis | |
| CN107137752B (en) | Preparation method of loofah sponge anti-adhesion wound dressing | |
| CN109432479B (en) | Antibacterial hemostatic hydrocolloid oily yarn and preparation method thereof | |
| RU2370270C1 (en) | Composition for wound treatment | |
| KR100608192B1 (en) | A Method for Producing a Neutralized Chitosan Sponge for Wound Dressing and Scaffold, and the Chitosan Sponge produced by the same |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20211228 |