CN101700408A - Novel gluconacetobacter xylinus cellulose hydrogel dressing and manufacturing method thereof - Google Patents
Novel gluconacetobacter xylinus cellulose hydrogel dressing and manufacturing method thereof Download PDFInfo
- Publication number
- CN101700408A CN101700408A CN200910211117A CN200910211117A CN101700408A CN 101700408 A CN101700408 A CN 101700408A CN 200910211117 A CN200910211117 A CN 200910211117A CN 200910211117 A CN200910211117 A CN 200910211117A CN 101700408 A CN101700408 A CN 101700408A
- Authority
- CN
- China
- Prior art keywords
- cellulose
- gluconate pyracetobacillus
- water
- pyracetobacillus
- hydrogel
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention discloses a novel gluconacetobacter xylinus cellulose hydrogel dressing and a manufacturing method thereof. The hydrogel dressing comprises natural high polymer gluconacetobacter xylinus cellulose, and is additionally compounded with water-soluble chitin derivative with antibacterial and bacteriostatic properties; and water-soluble medicine can be added into the hydrogel dressing. The hydrogel dressing contains no additive, has good functions of antibacterium, diminishing inflammation, stopping bleeding, promoting wound healing and the like, and has the characteristics of high water content, good water-retaining property, strong toughness and good air permeability, thus meeting the requirement for treating various wounds by a wet method and being used for treating burn and scald, bedsore and surgery infection.
Description
Technical field
The present invention relates to a kind of gluconate pyracetobacillus cellulose hydrogel dressing and preparation method thereof.
Background technology
Hydrogel medical dressing is a kind of novel wound dressing that development in recent years is got up, compare with at present used clinically traditional gauze dressing etc., aerogel dressing can shorten the time of wound healing, alleviate patient's pain, promote wound better to heal, and air permeable humidity retaining, when change dressings more, can not destroy the wound tissue of having healed, and the transparent advantages such as healing state that can observe wound obtain the generally attention of various countries.
The preparation method of aerogel dressing mainly contains chemical method at present, irradiation method and freeze-thaw method etc., and the aerogel dressing of chemical method preparation is the more residual cross-linking agent of meeting usually, deleterious micromolecule chemical substance such as initiator.Irradiation method need drop into the high price irradiation apparatus, and the hydrogel mechanical strength of preparation is less, and its application is restricted.Freeze-thaw method is a kind ofly effectively to prepare the method that polyvinyl alcohol prepares hydrogel, but shortcoming such as manufacturing cycle is long, and range of application is narrow.
The gluconate pyracetobacillus cellulose is that some antibacterials of occurring in nature are cultivated a kind of gelatinous thin film of producing by fermentation.Different with common Plant fiber, the gluconate pyracetobacillus cellulose does not contain lignin and hemicellulose, it is combined into the thick fibre bundle of 40-60nm by diameter 3-4nm fento and is intertwined to form hyperfine network structure, its inside has many " ducts ", good ventilative, suction, water permeability are arranged, have very high-hydrophilic, can keep himself 60～700 times moisture.And the gluconate pyracetobacillus cellulose has high-tensile and elastic modelling quantity, and the anti-tear energy force rate polyethylene film and the polyoxyethylene film of film are eager to excel 5 times.In addition, the gluconate pyracetobacillus cellulose has good biocompatibility, and is good
Some advantages of biodegradability or the like, the gluconate pyracetobacillus cellulose is considered to the best fibre of nature
Dimension is plain, at food, and papermaking, medical science, fields such as advanced material have obtained using widely.
Summary of the invention
The purpose of this invention is to provide a kind of novel gluconate pyracetobacillus cellulose hydrogel dressing and preparation method thereof, it is strong that a kind of novel gluconate pyracetobacillus cellulose hydrogel dressing of the present invention has water absorption, and moisture retention is good, the transparency height, the intensity height, characteristics such as pliability is good, and permeability is good, and have antibiotic, antiinflammatory, functions such as hemostasis and promotion wound healing are that preparation is simple, a kind of novel gluconate pyracetobacillus cellulose hydrogel dressing that cost is low.
Technology solution of the present invention is as follows:
A kind of gluconate pyracetobacillus cellulose hydrogel dressing, it is characterized in that: each component content is gluconate pyracetobacillus cellulose: water-solubility chitin derivative: water soluble drug by mass: solvent=1～5: 0.1～5: 0.001～2: 88～99.
Described gluconate pyracetobacillus cellulose is a contained gluconate pyracetobacillus cellulose in the gluconate pyracetobacillus cellulose aquagel that makes by following production technology, and its concrete production stage is: (1), gluconate pyracetobacillus cultivated make gluconate pyracetobacillus cellulose aquagel culture medium; The gluconate pyracetobacillus cellulose aquagel is 20～35 ℃ by gluconate pyracetobacillus in temperature, and original ph is about under 3～6 the condition, adds glucose, the hydrogel culture medium that sucrose or other carbohydrates make for the base material fermentation culture; (2), get 0.1～1ml fluid medium with aseptic straw, splash in the peace pipe of containing bacterial strain, light shaking makes the dissolving of lyophilizing thalline be outstanding emulsus, draws whole thalline suspended emulsions, transplant on slant medium, cultivated 2～4 days at 20～35 ℃, it is transferred on the same slant medium, shifted once every 2～4 days, so repeat 5～8 times, finish the actication of culture process; (3), get the good slant strains of a ring activation and insert seed culture medium, in 20～35 ℃ is stir culture 18～36h under the magnetic stirring apparatus of 300～550rpm/min at rotating speed, insert again and cultivate 5～10 days in the fluid medium, obtain the gluconate pyracetobacillus cellulose aquagel.
Described water-solubility chitin derivative is water miscible chitin, water-soluble carboxymethyl chitin, water-soluble chitosan, water solublity carboxyl chitosan or water-soluble carboxymethyl chitosan.
Described water soluble drug is one or more in YUNNAN BAIYAO, Li Pusong, antibacterials polymyxin B, ciprofloxacin, antibiotic medicine dexamethasone, coagulant etamsylate or the tranexamic acid.
Described solvent is redistilled water, normal saline or phosphate neutral buffered.
A kind of preparation method of antimicrobial form gluconate pyracetobacillus cellulose hydrogel dressing comprises the steps:
A, gluconate pyracetobacillus cultivated make the gluconate pyracetobacillus cellulose aquagel;
B, separation are purified, and remove thermal source.Make the gluconate pyracetobacillus cellulose aquagel reach pharmaceutical grades through separating the residual media of removing tropina and sticking on the cellulose membrane of purifying;
C, partial dehydration, the gluconate pyracetobacillus cellulose aquagel after the dehydration with the weight ratio of the preceding gluconate pyracetobacillus cellulose aquagel that dewaters is: 1: 1.5～1: 10;
D, soak, part dried hydrogel film after the sterilization is immersed under aseptic condition to contain the quality percentage composition be 1～10% water-solubility chitin derivative redistilled water, in normal saline or the phosphate neutral buffered solution, or be immersed in the redistilled water of the water soluble drug that contains quality percentage composition 0.005～5%, in normal saline or the phosphate neutral buffered solution, or be immersed in that not only to contain the quality percentage composition be 1～10% water-solubility chitin derivative but also the redistilled water that contains the water soluble drug of quality percentage composition 0.005～5%, in normal saline or the phosphate neutral buffered solution, treat to take out after the swelling behavior balance;
E, partial dehydration, the weight ratio of hydrogel is when dehydration back hydrogel and swelling equilibrium: 1: 1.5～1: 10;
F, sterile packaged, 5～30 ℃ of preservations.
Described dewatering type is natural drying, centrifugally drain, vacuum drying, lyophilization or machinery are pressed and removed.
Gamma-radiation, pressure-steam sterilization or electron beam sterilization method are adopted in described sterilization.
Beneficial effect of the present invention is:
1, the gluconate pyracetobacillus cellulose is a kind of natural water gel film, is used for artificial skin or other medical materials have good development prospect, and it provides moistening environment to promote that wound heals better for wound.It is antibiotic that but the gluconate pyracetobacillus cellulose does not have itself, functions such as antiinflammatory, therefore pass through to introduce this natural Biodegradable polymer material of water miscible chitin derivativ in this patent, can make the gluconate pyracetobacillus cellulose hydrogel dressing have antibacterial anti hemorrhagic, promote the function of cell regeneration.And in this aerogel dressing, can add water soluble drug, make this aerogel dressing have good blood coagulation, functions such as antiinflammatory and promotion wound healing.The gluconate pyracetobacillus cellulose aquagel is a kind of natural hydrogel, does not contain any chemical addition agent, without any toxic and side effects, and good biocompatibility.
2, by the gluconate pyracetobacillus cellulose aquagel is introduced water miscible chitin derivativ, have antibacterial anti hemorrhagic, promote the function of cell regeneration, do not need to introduce other additives.
3, the aerogel dressing that the present invention relates to can be introduced water soluble drug, makes aerogel dressing have functions such as excellent antibiotic anti-inflammation hemostasia.
4, the present invention adopts the cellulosic method of culture of bacteria to prepare the gluconate pyracetobacillus cellulose aquagel, compare with irradiation method, freeze-thaw method, need the equipment of use few, equipment cost has only 1/10th of irradiation method, the production technology simple possible, with short production cycle, instant effect, production cost greatly reduces, the preparation efficiency height.
The specific embodiment
Below in conjunction with the specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used to the present invention is described and be not used in and limit the scope of the invention.Should be understood that in addition those skilled in the art can make various changes or modifications the present invention after the content of having read the present invention's instruction, these equivalent form of values fall within the application's appended claims institute restricted portion equally.
Embodiment 1:
(1), the gluconate pyracetobacillus cultivation is made the gluconate pyracetobacillus cellulose aquagel; The gluconate pyracetobacillus cellulose aquagel is 30 ℃ by gluconate pyracetobacillus (Gluconacetobacterxylinus) in temperature, original ph is about under 5 the condition, with glucose, sucrose or other carbohydrates are the hydrogel that basic fermentation culture makes; Concrete steps are as follows: 1, get the 0.6ml fluid medium with aseptic straw, splash in the peace pipe of containing bacterial strain, light shaking makes the dissolving of lyophilizing thalline be outstanding emulsus, draws whole thalline suspended emulsions, transplant on slant medium, cultivated 3 days at 30 ℃, it is transferred on the same slant medium, shifted once every 3 days, so repeat 6 times, finish the actication of culture process; 2, get the good slant strains of a ring activation and insert seed culture medium, in 30 ℃ be stir culture 24h under the magnetic stirring apparatus of 450rpm/min at rotating speed, insert again in the fluid medium and cultivated 7 days, obtain the gluconate pyracetobacillus cellulose aquagel.
(2), separate and purify, the removal thermal source.It is in 5% the NaOH aqueous solution that the gluconate pyracetobacillus cellulose aquagel is immersed in the quality percentage composition, adds 3h under 100 ℃ temperature.The reuse redistilled water washes repeatedly to neutrality.Make 3mm (± 0.1mm) thick hydrogel diaphragm;
(3), partial dehydration, adopt machinery to press eliminating method, the weight ratio of the gluconate pyracetobacillus cellulose aquagel before the gluconate pyracetobacillus cellulose aquagel after the dehydration and the dehydration is: 1: 1.5;
(4), soak, the part dried hydrogel film after the sterilization is immersed under aseptic condition to contain the quality percentage composition be that 2% water-soluble chitosan is dissolved in 4h in the redistilled water, treat to take out after the swelling behavior balance;
(5), partial dehydration, adopt vacuum drying method, the hydrogel diaphragm is put into vacuum drying oven, the weight ratio of hydrogel is when 37 ℃ of dry 20h dehydration back hydrogels and swelling equilibrium: 1: 1.5,
(6), sterile packaged, adopt the coated with aluminum plastic bag that can seal, encapsulating material.Through the gamma-radiation sterilization treatment, irradiation level is 20KGy.5-30 ℃ of preservation.
The aerogel dressing that obtains is that 2% water-soluble chitosan is formed by gluconate pyracetobacillus cellulose, the quality percentage composition of quality percentage composition 2%.
Embodiment 2:
(1), the gluconate pyracetobacillus cultivation is made the gluconate pyracetobacillus cellulose aquagel; The gluconate pyracetobacillus cellulose aquagel is 35 ℃ by gluconate pyracetobacillus (Gluconacetobacterxylinus) in temperature, original ph is about under 6 the condition, with glucose, sucrose or other carbohydrates are the hydrogel that basic fermentation culture makes; Concrete steps are as follows: 1, get the 0.2ml fluid medium with aseptic straw, splash in the peace pipe of containing bacterial strain, light shaking makes the dissolving of lyophilizing thalline be outstanding emulsus, draws whole thalline suspended emulsions, transplant on slant medium, cultivated 1 day at 35 ℃, it is transferred on the same slant medium, shifted once every 3 days, so repeat 10 times, finish the actication of culture process; 2, get the good slant strains of a ring activation and insert seed culture medium, in 35 ℃ be stir culture 20h under the magnetic stirring apparatus of 400rpm/min at rotating speed, insert again in the fluid medium and cultivated 10 days, obtain the gluconate pyracetobacillus cellulose aquagel.
(2), separate and purify, the removal thermal source.It is in 6% the NaOH aqueous solution that the gluconate pyracetobacillus cellulose aquagel is immersed in the quality percentage composition, adds 1h under 80 ℃ temperature.The reuse redistilled water washes repeatedly to neutrality.Make 2mm (± 0.1mm) thick hydrogel diaphragm;
(3), partial dehydration, adopt vacuum drying method, the hydrogel diaphragm is put into vacuum drying oven, 37 ℃ of dry 24h, the gluconate pyracetobacillus cellulose aquagel after the dehydration with the weight ratio of the preceding gluconate pyracetobacillus cellulose aquagel that dewaters is: 1: 5;
(4), soak, part dried hydrogel film after the sterilization is immersed under aseptic condition to contain the quality percentage composition be that 1% water solublity carboxyl chitosan and quality percentage composition are that 2% water soluble drug is dissolved in 4h in the normal saline, treats to take out after the swelling behavior balance;
(5), partial dehydration, adopt vacuum drying method, the hydrogel diaphragm is put into vacuum drying oven, the weight ratio of hydrogel is when 37 ℃ of dry 20h dehydration back hydrogels and swelling equilibrium: 1: 2,
(6), sterile packaged, adopt the coated with aluminum plastic bag that can seal, encapsulating material.Through the gamma-radiation sterilization treatment, irradiation level is 25KGy.5～30 ℃ of preservations.The aerogel dressing that obtains is that 1% water solublity carboxyl chitosan and quality percentage composition are that 2% water soluble drug is formed by gluconate pyracetobacillus cellulose, the quality percentage composition of quality percentage composition 1.5%.
Embodiment 3:
(1), through Fructus Ananadis comosi water by fermentation cultivation preparation, separate the back thickness of purifying is 4mm gluconate pyracetobacillus cellulose aquagel to the gluconate pyracetobacillus cellulose aquagel, with redistilled water it is washed till neutrality by gluconate pyracetobacillus.
(2), to be neutral gluconate pyracetobacillus cellulose aquagel machinery presses except that moisture, gluconate pyracetobacillus cellulose aquagel after the dehydration with the weight ratio of the preceding gluconate pyracetobacillus cellulose aquagel that dewaters is: 1: 10, with the dehydrated hydrogel's radiosterilization, the hydrogel that drying after will sterilizing under aseptic condition is then crossed is immersed in that to contain the quality percentage composition be in the 10% water miscible carboxymethyl chitosan normal saline, take out partial dehydration after reaching swelling equilibrium, the weight ratio of hydrogel is when dehydration back hydrogel and swelling equilibrium: 1: 5, sterile packaged, 5～30 ℃ of preservations.
Embodiment 4:
(1), through the preparation of Schramm and Hestrin culture medium fermentation culture, purify through separating by the hydrogel that obtains by gluconate pyracetobacillus for the gluconate pyracetobacillus cellulose aquagel, getting thickness is the 5mm hydrogel, with redistilled water it is washed till neutrality.
(2), will be neutral gluconate pyracetobacillus cellulose aquagel vacuum drying moisture, gluconate pyracetobacillus cellulose aquagel after the dehydration with the weight ratio of the preceding gluconate pyracetobacillus cellulose aquagel that dewaters is: 1: 2, with the dehydrated hydrogel's radiosterilization, the hydrogel that drying after will sterilizing under aseptic condition is then crossed is immersed in that to contain the quality percentage composition be in 10% the water miscible chitin normal saline, take out sterile packaged after reaching swelling equilibrium, 5～30 ℃ of preservations.
Embodiment 5:
(1), through Sucus Cocois fermentation culture preparation, purify through separating by the hydrogel that obtains by gluconate pyracetobacillus for the gluconate pyracetobacillus cellulose aquagel, getting thickness is the 5mm hydrogel, with redistilled water it is washed till neutrality.
(2), will be the lyophilization of neutral gluconate pyracetobacillus cellulose aquagel, gluconate pyracetobacillus cellulose aquagel after the dehydration with the weight ratio of the preceding gluconate pyracetobacillus cellulose aquagel that dewaters is: 1: 1.5, with the dehydrated hydrogel's radiosterilization, the hydrogel that drying after will sterilizing under aseptic condition is then crossed is immersed in that to contain the quality percentage composition be 1% water miscible chitin and containing in the YUNNAN BAIYAO redistilled water of quality percentage composition 0.005%, take out sterile packaged after reaching swelling equilibrium, 5～30 ℃ of preservations.
Embodiment 6:
(1), the gluconate pyracetobacillus cellulose aquagel cultivates preparation by gluconate pyracetobacillus through the Fructus Ananadis comosi water by fermentation, the hydrogel that obtains is purified through separating, getting thickness is the 4mm hydrogel, with redistilled water it is washed till neutrality.
(2), to be neutral gluconate pyracetobacillus cellulose aquagel machinery presses except that moisture, gluconate pyracetobacillus cellulose aquagel after the dehydration with the weight ratio of the preceding gluconate pyracetobacillus cellulose aquagel that dewaters is: 1: 10, with the dehydrated hydrogel's radiosterilization, the hydrogel that drying after will sterilizing under aseptic condition is then crossed is immersed in that to contain the quality percentage composition be 10% chitin and containing in the YUNNAN BAIYAO normal saline of quality percentage composition 3%, reach the dehydration of swelling equilibrium rear section, the weight ratio of hydrogel is when dehydration back hydrogel and swelling equilibrium: 1: 10, sterile packaged, take out sterile packaged, 5～30 ℃ of preservations.
Embodiment 7:
(1), through Sucus Cocois fermentation culture preparation, purify through separating by the hydrogel that obtains by gluconate pyracetobacillus for the gluconate pyracetobacillus cellulose aquagel, getting thickness is the 3mm hydrogel, with redistilled water it is washed till neutrality.
(2), will be the lyophilization of neutral gluconate pyracetobacillus cellulose aquagel, gluconate pyracetobacillus cellulose aquagel after the dehydration with the weight ratio of the preceding gluconate pyracetobacillus cellulose aquagel that dewaters is: 1: 8, with the dehydrated hydrogel's radiosterilization, the hydrogel that drying after will sterilizing under aseptic condition is then crossed is immersed in that to contain the quality percentage composition be 1% water-soluble chitosan and contains in the YUNNAN BAIYAO normal saline of quality percentage composition 4%, take out sterile packaged after reaching swelling equilibrium, 5～30 ℃ of preservations.
Embodiment 8:
(1), through Sucus Cocois fermentation culture preparation, separate the back thickness of purifying is 2mm gluconate pyracetobacillus cellulose aquagel to the gluconate pyracetobacillus cellulose aquagel by gluconate pyracetobacillus, with redistilled water with it
Be washed till neutrality.
(2), will be the lyophilization of neutral gluconate pyracetobacillus cellulose aquagel, gluconate pyracetobacillus cellulose aquagel after the dehydration with the weight ratio of the preceding gluconate pyracetobacillus cellulose aquagel that dewaters is: 1: 5, with the dehydrated hydrogel's radiosterilization, the hydrogel that drying after will sterilizing under aseptic condition is then crossed is immersed in that to contain the quality percentage composition be 1% water-soluble chitosan and containing in the dexamethasone redistilled water of quality percentage composition 5%, take out sterile packaged after reaching swelling equilibrium, 5～30 ℃ of preservations.
Claims (8)
1. gluconate pyracetobacillus cellulose hydrogel dressing, it is characterized in that: each component content is gluconate pyracetobacillus cellulose: water-solubility chitin derivative: water soluble drug by mass: solvent=1～5: 0.1～5: 0.001～2: 88～99.
2. gluconate pyracetobacillus cellulose hydrogel dressing as claimed in claim 1, it is characterized in that: described gluconate pyracetobacillus cellulose is a contained gluconate pyracetobacillus cellulose in the gluconate pyracetobacillus cellulose aquagel that makes by following production technology, and its concrete production stage is: (1), gluconate pyracetobacillus cultivated make gluconate pyracetobacillus cellulose aquagel culture medium; The gluconate pyracetobacillus cellulose aquagel is 20～35 ℃ by gluconate pyracetobacillus in temperature, and original ph is about under 3～6 the condition, adds glucose, the hydrogel culture medium that sucrose or other carbohydrates make for the base material fermentation culture; (2), get 0.1～1ml fluid medium with aseptic straw, splash in the peace pipe of containing bacterial strain, light shaking makes the dissolving of lyophilizing thalline be outstanding emulsus, draws whole thalline suspended emulsions, transplant on slant medium, cultivated 2～4 days at 20～35 ℃, it is transferred on the same slant medium, shifted once every 2～4 days, so repeat 5～8 times, finish the actication of culture process; (3), get the good slant strains of a ring activation and insert seed culture medium, in 20～35 ℃ is stir culture 18～36h under the magnetic stirring apparatus of 300～550rpm/min at rotating speed, insert again and cultivate 5～10 days in the fluid medium, obtain the gluconate pyracetobacillus cellulose aquagel.
3. a kind of novel gluconate pyracetobacillus cellulose hydrogel dressing as claimed in claim 1 is characterized in that: described water-solubility chitin derivative be water miscible chitin,
Water-soluble carboxymethyl chitin, water-soluble chitosan, water solublity carboxyl chitosan or water-soluble carboxymethyl chitosan.
4. a kind of novel gluconate pyracetobacillus cellulose hydrogel dressing as claimed in claim 1 is characterized in that: described water soluble drug is one or more in YUNNAN BAIYAO, Li Pusong, antibacterials polymyxin B, ciprofloxacin, antibiotic medicine dexamethasone, coagulant etamsylate or the tranexamic acid.
5. a kind of novel gluconate pyracetobacillus cellulose hydrogel dressing as claimed in claim 1 is characterized in that: described solvent is redistilled water, normal saline or phosphate neutral buffered.
6. the preparation method of an antimicrobial form gluconate pyracetobacillus cellulose hydrogel dressing is characterized in that: comprise the steps:
A, gluconate pyracetobacillus cultivated make the gluconate pyracetobacillus cellulose aquagel;
B, separation are purified, and remove thermal source.Make the gluconate pyracetobacillus cellulose aquagel reach pharmaceutical grades through separating the residual media of removing tropina and sticking on the cellulose membrane of purifying;
C, partial dehydration, the gluconate pyracetobacillus cellulose aquagel after the dehydration with the weight ratio of the preceding gluconate pyracetobacillus cellulose aquagel that dewaters is: 1: 1.5～1: 10;
D, soak, part dried hydrogel film after the sterilization is immersed under aseptic condition to contain the quality percentage composition be 1～10% water-solubility chitin derivative redistilled water, in normal saline or the phosphate neutral buffered solution, or be immersed in the redistilled water of the water soluble drug that contains quality percentage composition 0.005～5%, in normal saline or the phosphate neutral buffered solution, or be immersed in that not only to contain the quality percentage composition be 1～10% water-solubility chitin derivative but also the redistilled water that contains the water soluble drug of quality percentage composition 0.005～5%, in normal saline or the phosphate neutral buffered solution, treat to take out after the swelling behavior balance;
E, partial dehydration, the weight ratio of hydrogel is when dehydration back hydrogel and swelling equilibrium: 1: 1.5～1: 10;
F, sterile packaged, 5～30 ℃ of preservations.
7. the preparation method of a kind of novel gluconate pyracetobacillus cellulose hydrogel dressing as claimed in claim 6 is characterized in that: described dewatering type is natural drying, centrifugally drain, vacuum drying, lyophilization or machinery are pressed and removed.
8. the preparation method of a kind of novel gluconate pyracetobacillus cellulose hydrogel dressing as claimed in claim 6 is characterized in that: gamma-radiation, pressure-steam sterilization or electron beam sterilization method are adopted in described sterilization.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910211117A CN101700408A (en) | 2009-11-05 | 2009-11-05 | Novel gluconacetobacter xylinus cellulose hydrogel dressing and manufacturing method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910211117A CN101700408A (en) | 2009-11-05 | 2009-11-05 | Novel gluconacetobacter xylinus cellulose hydrogel dressing and manufacturing method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101700408A true CN101700408A (en) | 2010-05-05 |
Family
ID=42155351
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200910211117A Pending CN101700408A (en) | 2009-11-05 | 2009-11-05 | Novel gluconacetobacter xylinus cellulose hydrogel dressing and manufacturing method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101700408A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012003640A1 (en) * | 2010-07-09 | 2012-01-12 | 奈菲儿国际股份有限公司 | Membrane of biological fibers, and use thereof |
| CN103418018A (en) * | 2013-07-29 | 2013-12-04 | 南通大学 | Tea fungus synthesized bacterial cellulose pressure sore dressing as well as preparation method and application thereof |
| CN104324413A (en) * | 2014-11-10 | 2015-02-04 | 华玉叶 | Preparation method of hydrogel dressing |
| CN112877384A (en) * | 2021-03-26 | 2021-06-01 | 哈尔滨理工大学 | Preparation method of bacterial cellulose, bacterial cellulose-chitosan composite gel skin-care mask and preparation method thereof |
| CN115382003A (en) * | 2021-05-24 | 2022-11-25 | 海南光宇生物科技有限公司 | Biological cellulose surgical suture without stitches taking off |
-
2009
- 2009-11-05 CN CN200910211117A patent/CN101700408A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012003640A1 (en) * | 2010-07-09 | 2012-01-12 | 奈菲儿国际股份有限公司 | Membrane of biological fibers, and use thereof |
| CN102405293A (en) * | 2010-07-09 | 2012-04-04 | 奈菲儿国际股份有限公司 | Membrane of biological fibers, and use thereof |
| CN103418018A (en) * | 2013-07-29 | 2013-12-04 | 南通大学 | Tea fungus synthesized bacterial cellulose pressure sore dressing as well as preparation method and application thereof |
| CN104324413A (en) * | 2014-11-10 | 2015-02-04 | 华玉叶 | Preparation method of hydrogel dressing |
| CN112877384A (en) * | 2021-03-26 | 2021-06-01 | 哈尔滨理工大学 | Preparation method of bacterial cellulose, bacterial cellulose-chitosan composite gel skin-care mask and preparation method thereof |
| CN115382003A (en) * | 2021-05-24 | 2022-11-25 | 海南光宇生物科技有限公司 | Biological cellulose surgical suture without stitches taking off |
| CN115382003B (en) * | 2021-05-24 | 2024-01-30 | 海南光宇生物科技有限公司 | Biological cellulose operation suture line without disconnecting |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Zhang et al. | Alginate hydrogel dressings for advanced wound management | |
| CN102961777B (en) | Method for preparing porous compound type high permeability absorption hemostasis coating with modified nano-crystalline cellulose | |
| Bano et al. | Preparation, characterization and evaluation of glycerol plasticized chitosan/PVA blends for burn wounds | |
| Kundu et al. | Cellulose hydrogels: Green and sustainable soft biomaterials | |
| CN102727926B (en) | The preparation method of the composite wound dressing of a kind of polysaccharide and nanometer bacteria cellulose | |
| JP2008532662A (en) | Method for producing antimicrobial wound dressing and use thereof | |
| CN108853570B (en) | Hemostatic sponge and preparation method thereof | |
| Gupta et al. | Hydrogels for wound healing applications | |
| CN105820352A (en) | Method for preparing electropositive chitin nanofiber hydrogel and aerogel | |
| CN103044693A (en) | Preparation method for bacterial cellulose/polyvinyl alcohol composite hydrogel | |
| CN110639050A (en) | Silk fibroin nanofiber and preparation method of silver-loaded antibacterial dressing based on silk fibroin nanofiber | |
| CN104144692A (en) | Composition, preparation, and use of dense chitosan membrane materials | |
| Sheokand et al. | Natural polymers used in the dressing materials for wound healing: Past, present and future | |
| CN103755965A (en) | Epsilon-polylysine hydrogel and preparation method and application thereof | |
| CN103357060B (en) | Method for preparing bacterial cellulose composite fish collagen wound dressing | |
| CN101700408A (en) | Novel gluconacetobacter xylinus cellulose hydrogel dressing and manufacturing method thereof | |
| RU2437681C1 (en) | Wound covering with therapeutic action | |
| JP6276268B2 (en) | Active polymer layer comprising chitin derivative especially for bandage and use thereof | |
| CN104436291B (en) | A kind of Vaccarin/Bacterial cellulose dressing treating diabetic foot ulcer | |
| CN113061265A (en) | Polysaccharide hydrogel, preparation method and application thereof | |
| Yaşayan et al. | Natural polymers for wound dressing applications | |
| Zahra et al. | Exploring the recent developments of alginate silk fibroin material for hydrogel wound dressing: A review | |
| Guamba et al. | Cellulose-based hydrogels towards an antibacterial wound dressing | |
| CN101530629A (en) | Method for preparing photo-crosslinking chitosan hydrogel film | |
| CN110124082A (en) | Swelling type medical bio gel filler based on Polysaccharide from Portulaca oleracea and chromocor extract |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20100505 |