CN109010195A - A kind of whitening Wrinkle-and scar-removing repairs face cream and preparation method thereof - Google Patents
A kind of whitening Wrinkle-and scar-removing repairs face cream and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a kind of whitening Wrinkle-and scar-removings to repair face cream and preparation method thereof.The face cream includes following components: allantoin, glycerol, isopropyl myristate, caprylic/capric triglyceride, hydroxyethyl cellulose, alkyl poly glucoside, cetearyl glucoside, tocopherol succinate, plant extracts, liposome containing bioactie agent, okra polysaccharide, gynostemma pentaphylla polysaccharide, seed oil of Moringa oleigera, evening primrose oil, docosyl alcohol, Phenoxyethanol, Chlorphenesin, surplus are deionized water.Each ingredient of the face cream is mutually coordinated, scar of dispelling significant effect, it is highly-safe and without skin irritation, and the effect of have both the tender skin of moisturizing, antioxidant and anti-aging, pigmentation can be effectively suppressed, it lightens the stain, restores skin to nature, even skin tone, simultaneously can smooth out wrinkles, restore skin it is compact.
Description
Technical field
The invention belongs to cosmetic technical fields, and in particular to a kind of whitening Wrinkle-and scar-removing repairs face cream and its preparation side
Method.
Background technique
Scar completely voluntarily normal cannot be repaired since human skin soft tissue is seriously damaged, must be by fiber group
The part to repair and leave is knitted to change.The mechanism formed at present for scar is not yet fully apparent from, it is considered that is that wound is repaired
Fibroblastic a large amount of proliferation and Apoptosis inhibitor in multiple, the synthesis and degradation of collagen in extracellular matrix, relevant cell because
The abnormal expression etc. of son constitutes the Basic of Biology of scar formation.
Scar has not only broken up body surface beauty, it is also possible to influence the function of linked groups or organ, more serious person can also cause
Deformity.Increase with people to itself appearance attention rate, skin wound reparation becomes cosmetic industry and shaping industry is paid close attention to
Hot spot.At present for scar treatment mostly use operative treatment, compression therapy, steroid injection therapy, radiotherapy,
Laser therapy, Silica hydrogel therapy and biotherapy etc. are freezed, these methods are played generally through following three aspect except scar acts on: 1)
The non-functional scar tissue for decomposing, removing, substituting aging, fibrosis；2) life of normal skin in-situ regeneration duplication is established
State environment promotes cytothesis function；3) it is continuous conveying life fertile absorber, balances pigment, restore skin health state.It is above-mentioned
Method can be relieved scar hyperplasia, improve appearance, but cannot repair scar completely.
With the development of biotechnology, it was recognized that bioactive ingredients are applied to the quality of scar, significant effect,
And it is highly-safe, as the Chinese patent application of Publication No. CN 107375906A discloses a kind of scar repair agent and its preparation
Method, the scar repair agent is by cell factor liposome and oat polypeptide, snail juice, L-Pyrrolidone Carboxylic Acid sodium, four phosphorus
The raw materials compounding such as sour two guanosines, papaya extractives, obtains significant scar repair.For another example Publication No. CN
The Chinese patent application of 106727115A discloses a kind of face cream and preparation method thereof for hyperplastic scar reparation, the shield
Skin frost by recombinant interleukin-1 2 and onion bulb stem extract, Gotu Kola P.E, beta glucan, catechin, saualane,
The raw materials such as wheat-germ oil compounding, obtains good hyperplastic scar repairing effect.
Although emerging one after another in recent years for the various product for scar research and development of dispelling, scar of dispelling is significant in efficacy, highly-safe, simultaneous
Have a whitening wrinkle removing effect, and it is easy to use and can the product of industrialization production or relatively fewer, be not able to satisfy the demand in market.
Summary of the invention
The object of the present invention is to provide a kind of whitening Wrinkle-and scar-removings to repair face cream and preparation method thereof.The face cream
Each ingredient is mutually coordinated, has scar effect of significantly dispelling, while having both whitening, smoothing wrinkle, anti-ageing effect, and skin irritation
It is low, it is highly-safe.
The present invention is achieved by the following technical programs:
A kind of whitening Wrinkle-and scar-removing reparation face cream, by mass percentage, including following components: allantoin 0.5~
3%, glycerol 1~6%, isopropyl myristate 0.5~3%, caprylic/capric triglyceride 1~3%, hydroxyethyl cellulose 5~
8%, alkyl poly glucoside 1~3%, cetearyl glucoside 0.5~2%, tocopherol succinate 0.5~3%, plant
Extract 3~6%, liposome containing bioactie agent 12~15%, okra polysaccharide 0.5~1.5%, gynostemma pentaphylla polysaccharide 0.5
~1.5%, seed oil of Moringa oleigera 0.5~2%, evening primrose oil 0.5~2%, docosyl alcohol 0.5~2%, Phenoxyethanol 0.05~0.5%,
Chlorphenesin 0.05~0.5%, surplus are deionized water.
It is preferred that the whitening Wrinkle-and scar-removing repairs face cream, by mass percentage, including following components: allantoin
2%, glycerol 5%, isopropyl myristate 2%, caprylic/capric triglyceride 2%, hydroxyethyl cellulose 6%, the poly- grape of alkyl
Glucosides 2%, cetearyl glucoside 1.5%, tocopherol succinate 1.5%, plant extracts 5%, containing bioactivity because
Sub- liposome 14%, okra polysaccharide 1%, gynostemma pentaphylla polysaccharide 1%, seed oil of Moringa oleigera 1.5%, evening primrose oil 1.5%, docosyl alcohol
2%, Phenoxyethanol 0.15%, Chlorphenesin 0.15%, surplus are deionized water.
The plant extracts is Horsetail P.E, atractylodes chinensis and a thousand pieces of gold boisiana extract with (1~2): 1:(1.5
~3) mass ratio composition.
It is preferred that the plant extracts is Horsetail P.E, atractylodes chinensis and a thousand pieces of gold boisiana extract with 1.5:1:2.5
Mass ratio composition.
The liposome containing bioactie agent includes according to parts by weight following components: Radix Notoginseng callus tissue culture
15~25 parts of object filtrate, 5~15 parts of rose hip stem cell extract, 35~45 parts of soybean lecithin, 5~15 parts of cholesterol, dimension
4~8 parts and 5~7 parts of Tween-80 of raw element E.
It is preferred that the liposome containing bioactie agent includes according to parts by weight following components: Radix Notoginseng callus group
Knit 20 parts of culture filtrate, 10 parts of rose hip stem cell extract, 40 parts of soybean lecithin, 10 parts of cholesterol, 6 parts of vitamin E
With 6 parts of Tween-80.
The liposome containing bioactie agent is prepared by the following method:
(1) soybean lecithin, cholesterol, vitamin E and the Tween-80 of formula ratio are weighed, is mixed, dehydrated alcohol is added,
Dissolution is sufficiently stirred, obtains solution A；
(2) rose hip stem cell extract is dissolved in the PBS buffer solution that pH is 7.2, stirs to being completely dissolved, is made
Then the Radix Notoginseng callus tissue culture filtrate of formula ratio is added in the solution of 0.1~0.2mg/mL, be uniformly mixed, obtain solution
B；
(3) in the case where magnetic stirring apparatus is stirred continuously, solution B is injected into solution A with syringe, it is sufficiently mixed 0.5~
1h, then at 28~32 DEG C, rotary evaporation under conditions of 0.1~1MPa removes ethyl alcohol, then vibrates water in 30~35 DEG C of water-baths
Change 1~2h of reaction, obtain liposome suspension, using the partial size of syringe type filter adjustment liposome suspension to 100~
150nm is to get liposome containing bioactie agent.
The present invention provides the preparation method that whitening Wrinkle-and scar-removing described in one kind repairs face cream, and this method includes following step
It is rapid:
(1) by the isopropyl myristate of formula ratio, caprylic/capric triglyceride, tocopherol succinate, moringa seeds
Oil, evening primrose oil, docosyl alcohol are added into first reactor, are stirred and heated to 80~85 DEG C, obtain material A, keep the temperature spare；
(2) deionized water of formula ratio, allantoin, glycerol, hydroxyethyl cellulose, alkyl poly glucoside, spermaceti is hard
Aliphatic radical glucoside, okra polysaccharide, gynostemma pentaphylla polysaccharide are added into second reactor, are stirred and heated to 80~85 DEG C, slowly
The material A that step (1) obtains is added, stirs homogeneous, obtains material B；
(3) 40~45 DEG C are cooled to material B, be added the plant extracts of formula ratio, liposome containing bioactie agent,
Phenoxyethanol and Chlorphenesin, stir evenly, and are cooled to room temperature, discharging, filling to repair face cream to get whitening Wrinkle-and scar-removing.
The Radix Notoginseng callus tissue culture filtrate is made by following steps:
(1) using the tender blade of Radix Notoginseng plant of health as the explant of evoked callus, on superclean bench,
First with 70% alcohol solution dipping 5min, then with aseptic water washing 3~5 times, surface moisture is blotted with sterilizing filter paper, is cut into
The fritter of 0.3cm × 0.3cm is inoculated in MS+0.1~2mg/L 2,4-D+0.1~2mg/L BA+0.002~1mg/L TDZ+
Callus induction is carried out in the culture medium of 30~60g/L sucrose, condition of culture: light control is that 6h illumination and 6h dark replace
It carries out；1500~2000lx of intensity of illumination, 25 ± 2 DEG C of temperature, cultivation cycle is 45 days.
(2) the good callus of growth conditions for taking step (1) to obtain, with sterilized tweezers on superclean bench
It is divided into mung bean size, is seeded in subculture medium and is cultivated, subculture medium is MS+0.1~2mg/L NAA+
0.1~2mg/L BA+0.002~4mg/L TDZ, condition of culture: light control be 12h illumination and 12h dark alternately；Light
According to 1000~1500lx of intensity, 25 ± 2 DEG C of temperature, cultivates 4 weeks, take filtrate after being sieved through filter with 50 aim cells, filtrate uses 80 again
Aim cell is sieved through filter, takes filtrate, sterilizes to get Radix Notoginseng callus tissue culture filtrate.
The rose hip stem cell extract is made by following steps:
(1) the tender blade of rose hip is taken, on superclean bench, first with 70% alcohol solution dipping 5min, then uses nothing
Bacterium water rinses 3~5 times, blots surface moisture with sterilizing filter paper, is cut into the fritter of 0.3cm × 0.3cm, is inoculated in stem cell induction
It in culture medium, when inoculation, contacts vacuum side of blade in culture medium, cultivate 20~25 days at 25~29 DEG C, obtain deriving from shape
The rose hip stem cell of stratification, the stem cell induced medium composition are as follows: 2450~2500mg/L of potassium nitrate, zinc sulfate 2
~3mg/L, 210~230mg/L of inositol, 19~21mg/L of thiamines class, 135~145mg/L of L-Aspartic acid, L-arginine 160
~180mg/L, 78~85mg/L of glycine, 1.8~3mg/L of α-naphthylacetic acid, 10~12mg/L of sucrose, active carbon 450~
3.6~3.8mg/L of 550mg/L and agar, surplus are water, and pH value is 6.0~6.5.
(2) it will be transferred to liquid culture medium from cambial rose hip stem cell, 6~7 is cultivated at 28~32 DEG C
It, the composition of the fluid nutrient medium are as follows: 180~184mg/L of magnesium sulfate, 0.3~0.6mg/L of potassium iodide, boric acid 2~
194~220mg/L of 2.5mg/L inositol, 57~62mg/L of L-AA, 136~142mg/L of L-Aspartic acid, glycine 75
~85mg/L, 3~5mg/L of α-naphthylacetic acid, 32~34mg/L of sucrose, surplus are water, and pH value is 5.8~6.0.
It (3) is 1 × 10 with fresh liquid culture medium adjustment rose hip stem cells density5A/mL is inoculated in the life of 20L
The interior amplification of object reactor is cultivated, and the fresh liquid culture medium of 5L is added in bioreactor, cultivates at 25~27 DEG C, is being cultivated extremely
At the 7th day, the fresh liquid culture medium of 10L is added into bioreactor, continues culture 15 days, with 100 mesh after culture
Stainless steel mesh filtering, remove cell mass, residue etc., obtain rose hip stem cell suspension；
(4) it by rose hip stem cell suspension, is filtered with 1 μm of filter, removes culture medium, harvest the cell of retention；And it will
Cell is put into -80 DEG C of superfreeze 2h, then takes out defrosting 30min, repeats aforesaid operations 5 times, last time room temperature solution
After jelly, filtrate is collected, in being concentrated in rotating vacuum evaporator, i.e., after filter filtering with sonicator smudge cells
It can get rose hip stem cell extract.
Whitening Wrinkle-and scar-removing provided by the invention repairs face cream and contains a variety of active skull cap components, includes meadow pine extraction
Object, atractylodes chinensis, a thousand pieces of gold boisiana extract, Radix Notoginseng callus tissue culture filtrate, lanatechead saussurea herb with flower stem cell extract, rose hip are dry
Cell extract, okra polysaccharide, gynostemma pentaphylla polysaccharide, seed oil of Moringa oleigera and evening primrose oil etc., each ingredient is mutually coordinated, directly acts on
In skin scar tissue, promote cytothesis, promote the growth of collagen and elastomer, becomes rough keloid
Flat, diminution, while assisting proliferative cell damaged tissues and softening it, the calm pigment of hyperplastic tissue is excreted, heavily fortified point is made
Hard hyperplastic scar flattens softening, effectively desalinates scar color.In addition, above-mentioned active constituent is also the stroma cell of skin
Sufficient nutrition is provided, the reconstructed velocity of neoplastic skin tissue is greatly speeded up, repairs skin from inside to outside, promotes wound healing,
Promote the synthesis of skin collagen, elastin laminin, repair skin barrier, moisturizing water lock makes skin get well full shape
State.
The Horsetail P.E is rich in silicic acid and silicate, can be effectively supplemented micro element silicon, skin permeation surface layer, directly
Up to skin corium, collagen and elastin laminin are acted on, locks the little albumen of dermal collagen network, Conservative restoration dermal tissue,
Reinforce corium fabric network, cell is made to keep integrality.Atractylodes chinensis is rich in the substances such as atractylol, hinesol, sagittol,
It can promote blood circulation, strengthen capilary, accelerate cell metabolism, activating and regenerating Skin Cell, accelerate the conjunction of collagen
At.A thousand pieces of gold boisiana extract is rich in a variety of alkaloids, and there is significant anti-inflammation and sterilization, soft junction to dissipate hard, antalgesic-antipruritic effect, be conducive to
Scar reparation, and there is certain free radical scavenging ability, it can reduce damage of the free radical to DNA, there is DNA protection and repair
Function promotes the recovery of skin barrier function.Three of the above plant extracts is used in compounding to cytothesis function is restored, and is repaired
Skin barrier has apparent cooperative gain effect.In addition, the okra polysaccharide and gynostemma pentaphylla polysaccharide in formula are in addition to good
Good free radical scavenging ability, can enhance outside the DNA repairing effect of a thousand pieces of gold boisiana extract, also have significant moisture-retaining capacity, promote
Into the generation of cell adhesion protein, intercellular adhesive connection is reinforced, makes to form close water conservation network between cell, enhancing is asked
The water lock function of chaste tree extract.
Seed oil of Moringa oleigera and evening primrose oil have the function of softening, decompose, remove scar tissue, reduce between keratinocyte
Degree of adhesion promotes dead Skin Cell to be fallen off, and plant absolute oil often contains multivitamin, microelement and not
Saturated fatty acid can help skin metabolism, anti-inflammatory, promotion regeneration, promotion wound healing.In addition, seed oil of Moringa oleigera
Uv absorption property it is stronger, can prevent ultraviolet light from scar being caused to form pigementation, help is declined pigment.Evening primrose oil is anti-inflammatory
Significant effect inhibits scar proliferation, accelerates scar of dispelling, and enhancing a thousand pieces of gold boisiana extract promotes the recovery of skin barrier function.
Whitening Wrinkle-and scar-removing provided by the invention, which is repaired face cream and also compounded, liposome containing bioactie agent, the lipid
Contain two kinds of Radix Notoginseng callus tissue culture filtrate, rose hip stem cell extract bioactie agents in body, has powerful
Cell tissue repair function, it includes the epigenetic factor effectively can provide nutrient for mankind's adult stem cell, effectively make skin
Skin epidermal stem cells, corium stem cell, subcutaneous tissue stem cell activation, can reach skin corium, in plant stem cell activity at
Divide the power of regeneration that can promote skin, improves epidermis and corium from inside to outside, to protect and maintain the function of human adult cell,
So that skin histology is updated reparation and delay senescence, improve skin quality, restores the ecological environment of Clinical application of skin situ regeneration medical technique duplication.Its
In, Radix Notoginseng callus tissue culture filtrate contains a variety of n cell activity factors, suspend mode fibroblast can be quickly activated,
Promote cell division, proliferation, promote DNA Damage repair ability, promotes cell self-regeneration to update, make Skin Cell in a steady stream
New life, constantly displacement senile cell, exclude intracellular toxin, while promoting absorption of the cell to nutritional ingredient, from Skin Cell
Metabolism is set out, and is reached preferable anti-Wrinkle-and scar and is repaired effect；Rose hip stem cell extract has powerful anti-oxidant and anti-ageing
Old activity can increase the concentration of SOD in human plasma, and the ability of oxygen radical is resisted in enhancing, but also can be increased in skin
The concentration of collagen promotes cell to secrete Collagen material, adjusts collagenous fibres, enhances skin elasticity, fundamentally improves skin
The elasticity of skin and compact degree assign skin adequate water and SuperPro outlet capacity, play anti crease and anti senile effect.
Ingredient in inventive formulation is unless otherwise instructed commercial product.Wherein, Horsetail P.E is purchased from Chengdu
Jia Ye Biotechnology Co., Ltd；Atractylodes chinensis and a thousand pieces of gold boisiana extract are purchased from Xi'an Rayleigh Biotechnology Co., Ltd；
Okra polysaccharide is purchased from Shaanxi Sen Fu natural product Co., Ltd；Gynostemma pentaphylla polysaccharide is limited purchased from the high Sheng biological medicine science and technology in Shaanxi
Company；Seed oil of Moringa oleigera is purchased from Guangzhou Herba Artemisia ordosicae Biotechnology Co., Ltd；Evening primrose oil has purchased from the auspicious skin people biotechnology in Guangzhou
Limit company.
Compared with prior art, the invention has the following advantages:
(1) whitening Wrinkle-and scar-removing reparation face cream provided by the invention contains a variety of active skull cap components, and each ingredient is mutual
Coordinate, plays multiple skin effect, can effectively dispel scar, crease-resistant, moisturizing, whitening spot-removing, skin health state is restored, and
Property is mildly stablized, safe and non-stimulating.
(2) whitening Wrinkle-and scar-removing reparation face cream preparation method provided by the invention is simple, and process stabilizing is quality controllable,
It has a good application prospect.
Specific embodiment
The following examples are further illustrations of the invention, rather than limiting the invention.
The preparation of 1 Radix Notoginseng callus tissue culture filtrate of embodiment
Radix Notoginseng callus tissue culture filtrate is made by following steps:
(1) explant of the tender blade of Radix Notoginseng plant as evoked callus is chosen first to use on superclean bench
70% alcohol solution dipping 5min, then with aseptic water washing 4 times, blot surface moisture with sterilizing filter paper, be cut into 0.3cm ×
The fritter of 0.3cm is inoculated in MS+1mg/L 2, carries out in the culture medium of 4-D+1mg/L BA+0.5mg/L TDZ+40g/L sucrose
Callus induction, condition of culture: light control be 6h illumination and 6h dark alternately；Intensity of illumination 1500lx, temperature 25
± 2 DEG C, cultivation cycle is 45 days.
(2) the good callus of growth conditions for taking step (1) to obtain, with sterilized tweezers on superclean bench
It is divided into mung bean size, is seeded in subculture medium and is cultivated, subculture medium is MS+1mg/L NAA+1mg/L
BA+0.5mg/L TDZ, condition of culture: light control be 12h illumination and 12h dark alternately；Intensity of illumination 1000lx, temperature
25 ± 2 DEG C of degree is cultivated 4 weeks, is sieved through filter with 50 aim cells after culture, takes filtrate, and filtrate uses 80 aim cells to be sieved through again
Filter takes filtrate, sterilizes to get Radix Notoginseng callus tissue culture filtrate.
The preparation of 2 rose hip stem cell extract of embodiment
Rose hip stem cell extract is made by following steps:
(1) the tender blade of rose hip is taken, on superclean bench, first with 70% alcohol solution dipping 5min, then uses nothing
Bacterium water rinses 4 times, blots surface moisture with sterilizing filter paper, is cut into the fritter of 0.3cm × 0.3cm, is inoculated in stem cell induction training
It supports in base, when inoculation, contacts vacuum side of blade in culture medium, cultivate 25 days at 27 DEG C, obtain deriving from cambial thorn rose
Dried fruit cell, the described stem cell induced medium composition are as follows: potassium nitrate 2500mg/L, zinc sulfate 2mg/L, inositol 230mg/L,
Thiamines class 21mg/L, L-Aspartic acid 145mg/L, L-arginine 160mg/L, glycine 78mg/L, α-naphthylacetic acid 2mg/L, sugarcane
Sugared 12mg/L, active carbon 550mg/L and agar 3.8mg/L, surplus are water, pH value 6.5.
(2) it will be transferred to liquid culture medium from cambial rose hip stem cell, will be cultivated 7 days at 32 DEG C, it is described
Fluid nutrient medium composition are as follows: magnesium sulfate 184mg/L, potassium iodide 0.6mg/L, boric acid 2.5mg/L inositol 220mg/L, L- are anti-
Bad hematic acid 57mg/L, L-Aspartic acid 136mg/L, glycine 75mg/L, α-naphthylacetic acid 5mg/L, sucrose 32mg/L, surplus are
Water, pH value 5.8.
It (3) is 1 × 10 with fresh liquid culture medium adjustment rose hip stem cells density5A/mL is inoculated in the life of 20L
The interior amplification of object reactor is cultivated, and the fresh liquid culture medium of 5L is added in bioreactor, cultivates at 25~27 DEG C, is being cultivated extremely
At the 7th day, the fresh liquid culture medium of 10L is added into bioreactor, continues culture 15 days, with 100 mesh after culture
Stainless steel mesh filtering, remove cell mass, residue etc., obtain rose hip stem cell suspension；
(4) it by rose hip stem cell suspension, is filtered with 1 μm of filter, removes culture medium, harvest the cell of retention；And it will
Cell is put into -80 DEG C of superfreeze 2h, then takes out defrosting 30min, repeats aforesaid operations 5 times, last time room temperature solution
After jelly, filtrate is collected, in being concentrated in rotating vacuum evaporator, i.e., after filter filtering with sonicator smudge cells
It can get rose hip stem cell extract.
The preparation of 3 liposome containing bioactie agent of embodiment
The formula composition of the liposome containing bioactie agent includes component described in following table according to parts by weight:
The formula composition of 1 liposome containing bioactie agent of table
It is formulated the preparation step of 1 liposome containing bioactie agent:
(1) soybean lecithin, cholesterol, vitamin E and the Tween-80 of formula ratio are weighed, is mixed, dehydrated alcohol is added,
Dissolution is sufficiently stirred, obtains solution A；
(2) rose hip stem cell extract is dissolved in the PBS buffer solution that pH is 7.2, stirs to being completely dissolved, is made
Then the Radix Notoginseng callus tissue culture filtrate of formula ratio is added in the solution of 0.1mg/mL, be uniformly mixed, obtain solution B；
(3) in the case where magnetic stirring apparatus is stirred continuously, solution B is injected into solution A with syringe, is sufficiently mixed 1h, so
Afterwards at 30 DEG C, rotary evaporation under conditions of 0.5MPa removes ethyl alcohol, then hydration reaction 2h is vibrated in 35 DEG C of water-baths, obtains lipid
Liquid suspension, the partial size using syringe type filter adjustment liposome suspension contain bioactivity to 100~150nm
Factor liposome.
It is formulated the preparation step reference formulation 1 of 2-5 liposome containing bioactie agent.
The preparation of 4 whitening Wrinkle-and scar-removing of embodiment reparation face cream
The preparation that 4 whitening Wrinkle-and scar-removing of embodiment repairs face cream includes the component of weight percent meter shown in the following table 2:
The formula composition of 2 whitening Wrinkle-and scar-removing of table reparation face cream
Preparation step:
(1) by the isopropyl myristate of formula ratio, caprylic/capric triglyceride, tocopherol succinate, moringa seeds
Oil, evening primrose oil, docosyl alcohol are added into first reactor, are stirred and heated to 80 DEG C, obtain material A, keep the temperature spare；
(2) deionized water of formula ratio, allantoin, glycerol, hydroxyethyl cellulose, alkyl poly glucoside, spermaceti is hard
Aliphatic radical glucoside, okra polysaccharide, gynostemma pentaphylla polysaccharide are added into second reactor, are stirred and heated to 80 DEG C, are slowly added to
The material A that step (1) obtains stirs homogeneous, obtains material B；
(3) 40 DEG C are cooled to material B, be added the Horsetail P.E of formula ratio, atractylodes chinensis, a thousand pieces of gold boisiana extract,
Liposome containing bioactie agent, Phenoxyethanol and Chlorphenesin, stir evenly, and are cooled to room temperature, discharging, filling to get beauty
White Wrinkle-and scar-removing repairs face cream.
The preparation of 1 whitening Wrinkle-and scar-removing of comparative example reparation face cream
The preparation that 1 whitening Wrinkle-and scar-removing of comparative example repairs face cream includes the component of weight percent meter shown in the following table 3:
The formula composition of 3 whitening Wrinkle-and scar-removing of table reparation face cream
Preparation step reference implementation example 4.
The preparation of 2 whitening Wrinkle-and scar-removing of comparative example reparation face cream
The difference that 2 whitening Wrinkle-and scar-removing of comparative example repairs face cream and the formula of embodiment 41 is, is mentioned with apple stem cell
Object and rose stem cell extract is taken to replace liposome containing bioactie agent, formula specifically: by mass percentage, packet
Include following components: allantoin 2%, glycerol 5%, isopropyl myristate 2%, caprylic/capric triglyceride 2%, ethoxy are fine
Tie up plain 6%, alkyl poly glucoside 2%, cetearyl glucoside 1.5%, tocopherol succinate 1.5%, plant extract
It is object 5%, dried apple slices cell extract 7%, rose stem cell extract 7%, okra polysaccharide 1%, gynostemma pentaphylla polysaccharide 1%, peppery
The wooden seed oil 1.5%, evening primrose oil 1.5%, docosyl alcohol 2%, Phenoxyethanol 0.15%, Chlorphenesin 0.15%, surplus be go from
Sub- water.
The dried apple slices cell extract is the Phytocelltec of the happy biochemical corp's production of Switzerland's rice hundredTMMalus
Domestica apple stem cell is the liposomes product of exploitation, main component based on rare Switzerland apple stem cell
It is Switzerland's apple berry extract, xanthan gum, glycerol, lecithin, Phenoxyethanol and water.
The rose stem cell extract is the Phytocelltec of the happy biochemical corp's production of Switzerland's rice hundredTMAlp Rose
Rose leaf stem cell is the powder-product based on the wild rose leaf stem cell of Alps.Main component is high mountain rose cuckoo
Flower extract, different malt, lecithin, sodium benzoate, lactic acid and water.
Preparation step reference implementation example 4.
Test example one, human skin patch
The related request of " human skin patch " in 2015 editions " cosmetics health specifications " issued referring to the Ministry of Public Health
1-5 is formulated to the embodiment of the present invention 4 with measuring method, comparative example 1 is formulated 6-11, whitening Wrinkle-and scar-removing made from comparative example 2 is repaired
Multiple face cream is tested, respectively 0h, 6h, 12h, for 24 hours, 48h observe dermoreaction, the experimental result of all trial volunteers
It is negative findings, does not occur skin adverse reaction.It is non-stimulated to show that whitening Wrinkle-and scar-removing provided by the invention repairs face cream
Property, it is highly-safe.
Test example two, whitening are dispelled scar measure of merit
Screening trial volunteer 84, the age 25-35 years old, wherein male 60, women 24；All trial volunteer's hands
There is apparent scar at arm, meet subject enrollment standard, experiment can be cooperated very well.Trial volunteer is divided into 12 groups, often
7 people of group, wherein male 5, women 2, respectively test 1-5 group and comparison 1-7 group, wherein test 1-5 group uses embodiment
Whitening Wrinkle-and scar-removing made from 4 formula 1-5 repairs face cream, and comparison 1-6 group is formulated whitening made from 6-11 using comparative example 1 and removes
Wrinkle-and scar repairs face cream, and 7 groups of comparison are repaired face cream using whitening Wrinkle-and scar-removing made from comparative example 2.
Test method: the trial volunteer of each group region fixed in arm scar is dispelled using same amount of whitening smoothing wrinkle
Scar repairs face cream, dosage 2mg/cm2, morning, noon, evening, continuous use 28 days used skin roughness daily respectively using 3 times
Tester VISIO SCAN tests scarred skin and repairs face cream front and back trial volunteer's using whitening Wrinkle-and scar-removing of the present invention
Skin roughness is evaluated with tri- indexs of SEsm, SEsc and SEw, wherein SEsm is smoothness parameter, the depth with wrinkle
Degree and form are proportional, and SEsm value is bigger, and skin is more smooth；SEsc reflects skin roughness indirectly, and SEsc value is bigger, cuticula
Water content is lower, and scaling of skin is more, and skin seems more coarse；SEw is wrinkle parameter, proportional to wrinkle quantity and depth.
Wrinkle of skin is more, and the value of SEw is higher, the results are shown in Table 4.And the average thickness of scar before and after use is detected, it the results are shown in Table 5.Together
Shi Liyong human skin tester detects volunteer the 0th day and the 28th day scarred skin melanin content, assesses the whitening of product
Scar of dispelling effect, the results are shown in Table 6.
The scar effect assessment result of dispelling of 4 each group trial volunteer of table
It is removed the results show that test 1-5 group trial volunteer uses the embodiment of the present invention 4 to be formulated whitening made from 1-5 respectively
After Wrinkle-and scar is repaired face cream 28 days, the skin SEsm of scarring areas increases, compared with showing that the skin of subject is preceding with test,
Skin is relatively smooth；SEsc is reduced, and shows that the moisture content of skin of subject increases, scaling of skin is reduced, and skin is more smooth to be had
Elasticity；SEw is reduced, and shows that wrinkle of skin is reduced, and skin pattern tends to smooth.The above result shows that using whitening of the invention
Wrinkle-and scar-removing can make the reduction of scarred skin roughness after repairing face cream, and elasticity improves, and it is extensive that skin mechanism tends to normal skin
Multiple, effect is substantially better than comparison 1-7 group.
The scar effect assessment result of dispelling of 5 each group trial volunteer of table
| Group | Use preceding scar average thickness (mm) | Use rear scar average thickness (mm) |
| Test 1 group | 1.16 | 0.32 |
| Test 2 groups | 0.94 | 0.15 |
| Test 3 groups | 1.10 | 0.28 |
| Test 4 groups | 1.03 | 0.25 |
| Test 5 groups | 0.98 | 0.18 |
| Compare 1 group | 1.05 | 0.60 |
| Compare 2 groups | 1.08 | 0.65 |
| Compare 3 groups | 0.96 | 0.76 |
| Compare 4 groups | 1.07 | 0.49 |
| Compare 5 groups | 1.02 | 0.46 |
| Compare 6 groups | 0.97 | 0.37 |
| Compare 7 groups | 1.13 | 0.45 |
It is removed the results show that test 1-5 group trial volunteer uses the embodiment of the present invention 4 to be formulated whitening made from 1-5 respectively
After Wrinkle-and scar is repaired face cream 28 days, scar average thickness is significantly reduced, and effect is better than comparison 1-7 group.
The pigment lightening effect assessment result of scar of 6 each group trial volunteer of table
| Group | Use preceding scar area dermal melanin value | Use rear scar area dermal melanin value |
| Test 1 group | 280.42 | 202.04 |
| Test 2 groups | 279.84 | 206.95 |
| Test 3 groups | 278.55 | 202.35 |
| Test 4 groups | 281.04 | 210.02 |
| Test 5 groups | 277.62 | 203.58 |
| Compare 1 group | 278.20 | 229.70 |
| Compare 2 groups | 279.52 | 233.32 |
| Compare 3 groups | 280.20 | 249.15 |
| Compare 4 groups | 278.38 | 221.90 |
| Compare 5 groups | 279.54 | 227.24 |
| Compare 6 groups | 277.80 | 219.30 |
| Compare 7 groups | 280.64 | 216.90 |
The results show that the embodiment of the present invention 4, which is used continuously, in test 1-5 group trial volunteer respectively is formulated beauty made from 1-5
After white Wrinkle-and scar-removing is repaired face cream 28 days, the melanin content of scarring areas skin is substantially reduced, and effects are obviously excellent
In comparison 1-7 group, shows that whitening Wrinkle-and scar-removing of the invention repairs face cream and skin can be promoted to get well normal skin tone, offset
Except scar pigment, skin whitening has apparent effect.
Test example three eliminates striae of pregnancy measure of merit
Women trial volunteer 180 of 6~24 months after screening childbirth, the age 25-35 years old, all trial volunteer's tripes
The apparent striae of pregnancy of Pi Junyou, meets subject enrollment standard, can cooperate experiment very well.Trial volunteer is divided into 12 groups, often
15 people of group, respectively test 1-5 group and comparison 1-7 group, wherein test 1-5 group is formulated whitening made from 1-5 using embodiment 4
Wrinkle-and scar-removing repairs face cream, and comparison 1-6 group is formulated whitening Wrinkle-and scar-removing made from 6-11 using comparative example 1 and repairs face cream,
It compares 7 groups and repairs face cream using whitening Wrinkle-and scar-removing made from comparative example 2.
Test method: the trial volunteer of each group is each primary using whitening Wrinkle-and scar-removing reparation face cream respectively in sooner or later,
It is applied at striae of pregnancy when use with about 0.5 gram of hand picking face cream, uniformly applies expansion and come, it can be with hand gently while the exhibition of painting
Position is smeared in massage, massages about 5min, is helped skin by massage and is absorbed, and after continuous use 60 days, counts using effect, as a result
It is shown in Table 7.
The striae of pregnancy eradicating efficacy evaluation result of 7 each group trial volunteer of table
| Group | It eliminates (%) | Mitigate (%) | It is slight to mitigate (%) |
| Test 1 group | 6(40) | 8(53.3) | 1(6.7) |
| Test 2 groups | 4(26.7) | 8(53.3) | 3(20) |
| Test 3 groups | 5(33.3) | 8(53.3) | 2(13.3) |
| Test 4 groups | 4(26.7) | 6(40) | 5(33.3) |
| Test 5 groups | 5(33.3) | 6(40) | 4(26.7) |
| Compare 1 group | 1(6.7) | 8(53.3) | 6(40) |
| Compare 2 groups | 1(6.7) | 6(40) | 8(53.3) |
| Compare 3 groups | 0(0) | 11(73.3) | 4(26.7) |
| Compare 4 groups | 2(13.3) | 7(46.7) | 6(40) |
| Compare 5 groups | 1(6.7) | 11(73.3) | 3(20) |
| Compare 6 groups | 2(13.3) | 9(60) | 4(26.7) |
| Compare 7 groups | 3(20) | 9(60) | 3(20) |
The results show that the embodiment of the present invention 4, which is used continuously, in test 1-5 group trial volunteer respectively is formulated beauty made from 1-5
After white Wrinkle-and scar-removing is repaired face cream 60 days, the elimination factor of striae of pregnancy is up to 26.7~40%, and mitigation rate is up to 40~53.3%, effect
It is substantially better than comparison 1-7 group.Show that whitening Wrinkle-and scar-removing of the invention repairs face cream and can effectively mitigate wrinkle of skin, enhances skin
Skin elasticity has good effect to the elimination of striae of pregnancy.
The above is only the preferred embodiment of the present invention, it is noted that above-mentioned preferred embodiment is not construed as pair
Limitation of the invention, protection scope of the present invention should be defined by the scope defined by the claims..For the art
For those of ordinary skill, without departing from the spirit and scope of the present invention, several improvements and modifications can also be made, these change
It also should be regarded as protection scope of the present invention into retouching.
Claims (8)
1. a kind of whitening Wrinkle-and scar-removing repairs face cream, which is characterized in that by mass percentage, including following components: allantois
Element 0.5~3%, glycerol 1~6%, isopropyl myristate 0.5~3%, caprylic/capric triglyceride 1~3%, ethoxy are fine
Dimension element 5~8%, alkyl poly glucoside 1~3%, cetearyl glucoside 0.5~2%, tocopherol succinate 0.5~
3%, plant extracts 3~6%, liposome containing bioactie agent 12~15%, okra polysaccharide 0.5~1.5%, gynostemma pentaphylla
Polysaccharide 0.5~1.5%, seed oil of Moringa oleigera 0.5~2%, evening primrose oil 0.5~2%, docosyl alcohol 0.5~2%, Phenoxyethanol 0.05
~0.5%, Chlorphenesin 0.05~0.5%, surplus are deionized water.
2. whitening Wrinkle-and scar-removing according to claim 1 repairs face cream, which is characterized in that by mass percentage, packet
Include following components: allantoin 2%, glycerol 5%, isopropyl myristate 2%, caprylic/capric triglyceride 2%, ethoxy are fine
Tie up plain 6%, alkyl poly glucoside 2%, cetearyl glucoside 1.5%, tocopherol succinate 1.5%, plant extract
Object 5%, liposome containing bioactie agent 14%, gynostemma pentaphylla polysaccharide 1%, seed oil of Moringa oleigera 1.5%, is shown in the moon okra polysaccharide 1%
Careless oil 1.5%, docosyl alcohol 2%, Phenoxyethanol 0.15%, Chlorphenesin 0.15%, surplus is deionized water.
3. whitening Wrinkle-and scar-removing according to claim 1 or 2 repairs face cream, which is characterized in that the plant extract
Object be Horsetail P.E, atractylodes chinensis and a thousand pieces of gold boisiana extract with (1~2): 1:(1.5~3) mass ratio form.
4. whitening Wrinkle-and scar-removing according to claim 3 repairs face cream, which is characterized in that the plant extracts is
Horsetail P.E, atractylodes chinensis and a thousand pieces of gold boisiana extract are formed with the mass ratio of 1.5:1:2.5.
5. whitening Wrinkle-and scar-removing according to claim 1 or 2 repairs face cream, which is characterized in that described is living containing biology
Sex factor liposome includes according to parts by weight following components: 15~25 parts of Radix Notoginseng callus tissue culture filtrate, rose hip
5~15 parts of stem cell extract, 35~45 parts of soybean lecithin, 5~15 parts of cholesterol, 4~8 parts of vitamin E and Tween-80 5
~7 parts.
6. whitening Wrinkle-and scar-removing according to claim 5 repairs face cream, which is characterized in that it is described containing bioactivity because
Sub- liposome, according to parts by weight, include following components: 20 parts of Radix Notoginseng callus tissue culture filtrate, rose hip stem cell mention
Take 10 parts of object, 40 parts of soybean lecithin, 10 parts of cholesterol, 6 parts of vitamin E and 6 parts of Tween-80.
7. whitening Wrinkle-and scar-removing according to claim 5 repairs face cream, which is characterized in that it is described containing bioactivity because
The preparation of sub- liposome the following steps are included:
(1) soybean lecithin, cholesterol, vitamin E and the Tween-80 of formula ratio are weighed, is mixed, dehydrated alcohol is added, sufficiently
Stirring and dissolving obtains solution A；
(2) rose hip stem cell extract is dissolved in the PBS buffer solution that pH is 7.2, stirred to being completely dissolved, it is made 0.1~
Then the Radix Notoginseng callus tissue culture filtrate of formula ratio is added in the solution of 0.2mg/mL, be uniformly mixed, obtain solution B；
(3) in the case where magnetic stirring apparatus is stirred continuously, solution B is injected into solution A with syringe, is sufficiently mixed 0.5~1h, so
Afterwards at 28~32 DEG C, rotary evaporation under conditions of 0.1~1MPa removes ethyl alcohol, then oscillation aquation is anti-in 30~35 DEG C of water-baths
1~2h is answered, liposome suspension is obtained, using the partial size of syringe type filter adjustment liposome suspension to 100~150nm,
Up to liposome containing bioactie agent.
8. a kind of method for preparing whitening Wrinkle-and scar-removing described in claim 1 and repairing face cream, which is characterized in that including following
Step:
(1) by the isopropyl myristate of formula ratio, caprylic/capric triglyceride, tocopherol succinate, seed oil of Moringa oleigera, the moon
See that careless oil, docosyl alcohol are added into first reactor, is stirred and heated to 80~85 DEG C, obtains material A, keep the temperature spare；
(2) by the deionized water of formula ratio, allantoin, glycerol, hydroxyethyl cellulose, alkyl poly glucoside, cetearyl
Glucoside, okra polysaccharide, gynostemma pentaphylla polysaccharide are added into second reactor, are stirred and heated to 80~85 DEG C, are slowly added to
The material A that step (1) obtains stirs homogeneous, obtains material B；
(3) 40~45 DEG C are cooled to material B, the plant extracts, liposome containing bioactie agent, benzene oxygen of formula ratio is added
Ethyl alcohol and Chlorphenesin, stir evenly, and are cooled to room temperature, discharging, filling to repair face cream to get whitening Wrinkle-and scar-removing.
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Application publication date: 20181218 |
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| WW01 | Invention patent application withdrawn after publication |