CN107581067A - A kind of little Hua all ages orchid species seedling aseptic seeding propagation methods - Google Patents
A kind of little Hua all ages orchid species seedling aseptic seeding propagation methods Download PDFInfo
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Abstract
A kind of little Hua all ages orchid species seedling aseptic seeding propagation methods, including by after little Hua all ages blue capsule sterilization, elution forms seed suspension liquid, is then cultivated in once-seedling forming culture medium, is then stepped up intensity of illumination, cultivates to obtain seedling, seedlings and rooted seedling；The rooted seedling is taken out from blake bottle, cleans after being attached to the culture medium in the rooted seedling, is soaked with liquor potassic permanganate, cultivation is in matrix after the root for then drying rooted seedling to rooted seedling bleaches.The present invention carries out aseptic seeding using ripe capsule, once cultivates seedling, has the characteristics that reproduction speed is fast, seedling is best in quality, growth cost is low, it is only necessary to which simple tissue culturing equipment can is completed.
Description
Technical field
The invention belongs to plant protection biology and biological technical field, all ages orchid species seedling is sterile by more particularly to a kind of little Hua
Seed propagation method.
Background technology
Little Hua is all ages blue（Vanda coerulescens Griff.）It is orchid family all ages epidendrum, is distributed in India east
The western Zhenkang of the north, Burma, Thailand, and Chinese yunnan, billows deep blue, Mojiang, Simao, Jinghong, Menhai, Menla, Yuanjiang River, Yuanyang etc.
County；Height above sea level 700-1600 rice is born in, is grown nonparasitically upon another plant in sparse woods on trunk.For epiphytic orchid, stem is sturdy, long 8-30 centimetres, Ye Duomei, and two
Row, tip, which has, irregularly to be incised；Raceme 1-2, up to 36 centimetres, tool is spent more, and pattern white is with light blue, florescence 3-5
Month, Chinese Second Class Key Protected Plant, have important breeding, view and admire, scientific research, protection etc. are worth.But due to the felling that people are excessive
And its growing environment changes, all ages orchid species source is rare by the little Hua in field, thus needs to carry out it artificial propagation, a side
On the other hand the seedling of artificial propagation can be returned original producton location and put in a suitable place to breed by face in addition to the needs of meeting market and breeding, scientific research,
Its energy natural propagation is made, expands its population quantity, admirably protects the diversity of the species.
There is no little Hua all ages sapling multiplication patent and document report also in the world at present.It is therefore desirable to design a kind of little Hua
All ages orchid species seedling aseptic seeding propagation method.
The content of the invention
It is an object of the invention to provide a kind of little Hua all ages orchid species seedling aseptic seeding propagation methods.
In order to achieve the above objects and other related objects, technical scheme provided by the invention is：A kind of little Hua all ages orchid speciess
Seedling aseptic seeding propagation method, comprises the following steps：
The first step：By little Hua, all ages blue capsule with alcohol-pickled, is then sterilized with mercuric chloride solution, next with aseptic water washing,
The moisture on the capsule surface is blotted with aseptic filter paper under aseptic condition；Then capsule is cut with aseptic operation knife, by capsule
Seed in fruit is eluted to one and accommodated in the container of sterilized water, and seed suspension liquid is made, then with sterilized water by the seed
Suspension is adjusted to the inoculation seed suspension liquid of 20 ~ 30 seed/milliliters of concentration；
Second step：Under aseptic condition, the inoculation is moved to one with seed suspension liquid and accommodates once-seedling forming solid medium
In orchid blake bottle, the seed amount in each orchid blake bottle is 40 ~ 60；Every liter of once-seedling forming culture medium contains
Have 1 ~ 2g spend precious No. 1,1~2g spend precious No. 2,0.5~2g peptone, 40 ~ 80g mashed potatoes, 50 ~ 100g banana
Mud, 10 ~ 20g apple butter, 500 ~ 1000mg activated carbon, 80 ~ 120mg inositol, 1.5 ~ 2.5mg glycine, 0.05 ~
0.2mg thiamine hydrochloride, 0.4 ~ 0.8mg puridoxine hydrochloride, 0.4 ~ 0.8mg nicotinic acid, 0.05 ~ 0.1mg 6- benzyls are fast
Purine, 0.2 ~ 2 mg methyl α-naphthyl acetate, 15~30g sucrose, 6~7g agar；The pH values of the once-seedling forming culture medium are 5.4-
5.6；
3rd step：By the blake bottle temperature be 23 ~ 27 DEG C, intensity of illumination be 100~1000 Lux under conditions of culture 60 ~
90 days, seed is set to sprout to form protocorm, protocorm sends out roots obtains seedling with blade；
4th step：By the seedling at 24 ~ 28 DEG C, intensity of illumination is cultivated under conditions of being 1000~3000 Lux, obtains height
For the seedlings that 3 ~ 5cm, blade quantity are 3~5, the quantity of coring is 3~5, the length of root is 1~3 centimetre；
5th step：By the seedlings at 24 ~ 28 DEG C, intensity of illumination is cultivated under conditions of being 3000~5000 Lux, obtains height
For 5 ~ 7cm, blade quantity be more than 4, the quantity of coring is more than 5, a diameter of more than 3 millimeters of rooted seedling of root；
6th step：The rooted seedling is taken out from blake bottle, cleans after being attached to the culture medium in the rooted seedling, uses permanganic acid
Potassium solution soaks, and cultivation is in matrix after the root for then drying rooted seedling to rooted seedling bleaches.
Preferably technical scheme is：By little Hua, all ages blue capsule volume fraction is alcohol-pickled 20 ~ 40 seconds of 70%, so
Sterilized 15 ~ 20 minutes with the mercuric chloride solution that mass fraction is 0.1 ~ 0.3% afterwards, next use aseptic water washing 4 ~ 5 times, then at nothing
The moisture on the capsule surface is blotted under the conditions of bacterium with aseptic filter paper；Then capsule is cut with aseptic operation knife, by capsule
Seed is eluted in a container for accommodating 200 milliliters of sterilized waters, and seed suspension liquid is made.
Preferably technical scheme is：In the 6th step, with the liquor potassic permanganate immersion 4 ~ 6 that mass fraction is 0.1 ~ 0.3%
Minute.
Because above-mentioned technical proposal is used, the present invention this have the advantage that compared with prior art：
The present invention carries out aseptic seeding using ripe capsule, once cultivates seedling, has that reproduction speed is fast, seedling quality is excellent
Good, the features such as growth cost is low, it is only necessary to which simple tissue culturing equipment can is completed.
Embodiment
Embodiments of the present invention are illustrated by particular specific embodiment below, those skilled in the art can be by this implementation
Content disclosed by example understands other advantages and effect of the present invention easily.
Embodiment 1：A kind of little Hua all ages orchid species seedling aseptic seeding propagation methods
（1）Material：The little Hua all ages blue progress artificial pollinations of robust growth, 150 days selection kind subbases after pollination are chosen when blooming
This maturation and uncracked capsule are that explant is used for aseptic seeding.
（2）It is prepared by seed suspension liquid：It is explant to choose the capsule that fruit age is 150 days, in the alcohol that volume fraction is 70%
Middle immersion 30 seconds, then sterilized 18 minutes with the mercuric chloride solution that mass fraction is 0.1%, aseptic water washing 4 times, in superclean bench
The moisture on capsule surface is blotted under upper aseptic condition with aseptic filter paper.Then capsule is cut with aseptic operation knife, by capsule
Seed is all eluted in a triangular flask equipped with 200 milliliters of sterilized waters, and the seed of each capsule, which is correspondingly eluted to one, bottled to be had
The triangular flask of 200 milliliters of sterilized waters, seed suspension liquid is made.The detection of seed density is carried out to each capsule seed suspension liquid,
Its seed amount, every bottle of seed suspension liquid are calculated under biomicroscope drawing 20 microlitres with sterile liquid-transfering gun in the case of shaking up
Repetition takes 10 times and calculates its average every milliliter of seed number.Kind is further diluted to sterilized water according to every bottle of suspension seed concentration
Sub- concentration is the seed suspension liquid of the inoculation of 30/milliliter.
（3）Aseptic seeding：2 milliliters of the seed suspension liquid of inoculation is drawn to equipped with 150 millis with liquid-transfering gun under aseptic condition
In the orchid blake bottle of the once-seedling forming solid medium risen, every bottle of little Hua all ages blue seed amounts 60.Once-seedling forming is trained
Support base：Every liter, containing No. 1 1.5g of treasured is spent, is spent treasured No. 2 1.5g, peptone 2g, mashed potatoes 80 g, banana puree 100g, apple butter 10g,
Activated carbon 500mg, inositol 100mg, glycine 2.0mg, thiamine hydrochloride（VB1）0.2mg, puridoxine hydrochloride（VB6）0.5mg,
Nicotinic acid 0.5mg, 6- benzyl purine（6-BA）0.05 milligram, methyl α-naphthyl acetate（NAA）1.0mg, sucrose 20g, agar 6.0g, pH 5.4.
（4）Build natral light cahure room：The culturing room of printing opacity is built up using safety glass, is equipped with shading system, cooling
Equipment, culturing rack.Shading system includes inside and outside sunshade net, and the light of culture building overlay culturing rack is made by sunshade net inside and outside regulation and control
It is 3000~5000 Lux according to intensity, second and third layer of culturing rack intensity of illumination of top layer down is 1000~3000 Lux, bottom
The 4th layer of Lux of intensity of illumination 100~1000 i.e. under top layer.Cooling system uses air conditioner for both cooling and heating, can be by glass culturing room
Temperature control is at 20~30 DEG C.A width of 60 centimetres of culturing rack, a height of 200 centimetres, 10 centimetres are the 4th layer from the ground, and layer is away from 60 lis
Rice, totally four layers, culturing rack length can be depending on the size of culturing room.
（5）Protocorm Fiber differentiation：Seed suspension liquid is uniformly distributed in media surface, this blake bottle is placed entirely certainly
On the 4th floor culturing rack in right optical culture room, its intensity of illumination is kept to exist in 100~1000 Lux, holding culture room temperature
(25±2)℃.Cultivated 60~90 days under the conditions of this, seed is sprouted to form protocorm, and protocorm sends out roots and blade.Cultivation temperature
For (26 ± 2) DEG C.
（6）Seedling culture：Seedling is moved to second or third layer of culturing rack, strengthens its natral light cahure, is promoted
Seedling grows, and this period incubation time is 90 days, and seedling grows up to seedlings, 3~5cm of height of seedling, 3~5 blades, 3~5 corings,
1~3 centimetre of root long.Cultivation temperature is (26 ± 2) DEG C.
（7）Stock breeding：Seedlings are moved to the first layer of culturing rack, is cultivated under high natural light light intensity, both may be used
To promote the growth of seedlings, the effect of hardening can be reached again.This period cultivates 60 days, height of seedling 5~7cm, 4 with blade,
More than 3 roots, thick more than the 3.0mm of stem, reach the standard of emergence.Cultivation temperature is (26 ± 2) DEG C.
（8）Bottle seedling is transplanted：During transplanting, rooted seedling is taken out from blake bottle, after the culture medium for cleaning attachment, uses mass fraction
5 min are soaked for 0.1% liquor potassic permanganate, then rooted seedling is dried to root and bleached, planting matrix is using sterilized place
The sphagna of reason, pay attention to keeping suitable humidity and temperature, be placed at shady and cool ventilation and cultivate, survival rate is up to more than 95%.30 d or so
Plant after surviving can produce new root system, now may move into greenhouse and carry out normal water, fertilizer, pencil reason.
Embodiment 2：A kind of little Hua all ages orchid species seedling aseptic seeding propagation methods
（1）Material and artificial pollination：All ages blue season of flowers, selection confirm to invest its different flower together after pollen is vibrant Yu little Hua
On another colored column cap of one plant.Bagging after pollination, bag is removed after about 1 week, hang up label, pollination date and awarded on label
The information such as powder people.Pay attention to the developmental state of observation hybridization capsule, can gather capsule after 4 months after pollination carries out aseptic seeding.
（2）It is prepared by seed suspension liquid：It is explant to choose the capsule that fruit age is 140 days, in the alcohol that volume fraction is 70%
Middle immersion 30 seconds, then sterilized 20 minutes with the mercuric chloride solution that mass fraction is 0.1%, aseptic water washing 5 times, in superclean bench
The moisture on capsule surface is blotted under upper aseptic condition with aseptic filter paper.Then capsule is cut with aseptic operation knife, by capsule
Seed is all eluted in a triangular flask equipped with 200 milliliters of sterilized waters, and the seed of each capsule, which is correspondingly eluted to one, bottled to be had
The triangular flask of 200 milliliters of sterilized waters, seed suspension liquid is made.The detection of seed density is carried out to each capsule seed suspension liquid,
Its seed amount, every bottle of seed suspension liquid are calculated under biomicroscope drawing 20 microlitres with sterile liquid-transfering gun in the case of shaking up
Repetition takes 10 times and calculates its average every milliliter of seed number.Kind is further diluted to sterilized water according to every bottle of suspension seed concentration
Sub- concentration is the seed suspension liquid of the inoculation of 25/milliliter.
（3）Aseptic seeding：2 milliliters of the seed suspension liquid of inoculation is drawn to equipped with 150 millis with liquid-transfering gun under aseptic condition
In the orchid blake bottle of the once-seedling forming solid medium risen, every bottle of little Hua all ages blue seed amounts 50.Once-seedling forming is trained
Support base：Every liter, containing No. 1 2.0g of treasured is spent, is spent treasured No. 2 2.0g, peptone 2g, mashed potatoes 40 g, banana puree 80g, apple butter 10g,
Activated carbon 500mg, inositol 100mg, glycine 2.0mg, thiamine hydrochloride（VB1）0.2mg, puridoxine hydrochloride（VB6）0.5mg,
0.05 milligram of nicotinic acid 0.5mg, 6- benzyl purine, methyl α-naphthyl acetate（NAA）1.0mg, sucrose 20g, agar 6.0g, pH 5.4.
（4）Build natral light cahure room：The culturing room of printing opacity is built up using safety glass, is equipped with shading system, cooling
Equipment, culturing rack.Shading system includes inside and outside sunshade net, and the light of culture building overlay culturing rack is made by sunshade net inside and outside regulation and control
It is 3000~5000 Lux according to intensity, second and third layer of culturing rack intensity of illumination of top layer down is 1000~3000 Lux, bottom
The 4th layer of Lux of intensity of illumination 100~1000 i.e. under top layer.Cooling system uses air conditioner for both cooling and heating, can be by glass culturing room
Temperature control is at 20~30 °C.A width of 60 centimetres of culturing rack, a height of 200 centimetres, 10 centimetres are the 4th layer from the ground, and layer is away from 60 lis
Rice, totally four layers, culturing rack length can be depending on the size of culturing room.
（5）Protocorm Fiber differentiation：Seed suspension liquid is uniformly distributed in media surface, this blake bottle is placed entirely certainly
On the 4th floor culturing rack in right optical culture room, its intensity of illumination is kept to exist in 100~1000 Lux, holding culture room temperature
(25±2)℃.Cultivated 60~90 days under the conditions of this, seed is sprouted to form protocorm, and protocorm sends out roots and blade.Cultivation temperature
For (26 ± 2) DEG C.
（6）Seedling culture：Seedling is moved to second or third layer of culturing rack, strengthens its natral light cahure, is promoted
Seedling grows, and this period incubation time is 90 days, and seedling grows up to seedlings, 3~5cm of height of seedling, 3~5 blades, 3~5 corings,
1~3 centimetre of root long.Cultivation temperature is (26 ± 2) DEG C.
（7）Stock breeding：Seedlings are moved to the first layer of culturing rack, is cultivated under high natural light light intensity, both may be used
To promote the growth of seedlings, the effect of hardening can be reached again.This period cultivates 60 days, height of seedling 5~7cm, 4 with blade,
More than 3 roots, thick more than the 3.0mm of stem, reach the standard of emergence.Cultivation temperature is (26 ± 2) DEG C.
（8）Bottle seedling is transplanted：During transplanting, rooted seedling is taken out from blake bottle, after the culture medium for cleaning attachment, uses one thousandth
Liquor potassic permanganate soak 5 min, then rooted seedling is dried to root and bleached, planting matrix use sterile-processed water
Tongue, pay attention to keeping suitable humidity and temperature, be placed at shady and cool ventilation and cultivate, survival rate is up to more than 98%.After 30 d or so are survived
Plant can produce new root system, now may move into greenhouse carry out normal water, fertilizer, pencil reason.
Embodiment 3：A kind of little Hua all ages orchid species seedling aseptic seeding propagation methods
Material：It is all ages blue to be stored in little Hua in South China Botanical Garden Chinese Academy of Sciences glasshouse.
Artificial pollination：Yu little Hua all ages blue seasons of flowers, choose and confirm its different flower is invested into same plant after pollen is vibrant
On another colored column cap of strain.Bagging after pollination, bag is removed after about 1 week, hang up label, pollination date and pollination people on label
Etc. information.Pay attention to the developmental state of observation hybridization capsule, can gather capsule after 4 months after pollination carries out aseptic seeding.
A kind of little Hua all ages orchid species seedling aseptic seeding propagation methods, comprise the following steps：
The first step：It is explant to choose the capsule that fruit age is 120-150 days, and by little Hua, all ages blue capsule is with alcohol-pickled, then
Sterilized with mercuric chloride solution, next with aseptic water washing, the capsule surface is blotted with aseptic filter paper under aseptic condition
Moisture；Then capsule is cut with aseptic operation knife, the seed in capsule is eluted to one and accommodated in the container of sterilized water, is made
Seed suspension liquid, then inoculation that the seed suspension liquid is adjusted to 20 seed/milliliters of concentration with sterilized water are hanged with seed
Supernatant liquid；
Second step：Under aseptic condition, the inoculation is moved to one with seed suspension liquid and accommodates once-seedling forming solid medium
In orchid blake bottle, the seed amount in each orchid blake bottle is 40；Every liter of once-seedling forming culture medium contains
1g spend precious No. 1,2g spend precious No. 2,1.2g peptone, 60g mashed potatoes, 50g banana puree, 20g apple butter,
1000mg activated carbon, 90mg inositol, 1.5mg glycine, 0.2mg thiamine hydrochloride, 0.7mg puridoxine hydrochloride,
0.7mg nicotinic acid, 0.1mg 6- benzyl purines, 0.25mg methyl α-naphthyl acetate, 20g sucrose, 6g agar；The once-seedling forming
The pH values of culture medium are 5.5；
Build natral light cahure room：The culturing room of printing opacity is built up using safety glass, is equipped with shading system, cooling system, training
Support frame.Shading system includes inside and outside sunshade net, and the intensity of illumination of culture building overlay culturing rack is made by sunshade net inside and outside regulation and control
For 3000~5000 Lux, second and third layer of culturing rack intensity of illumination of top layer down is 1000~3000 Lux, and bottom is top layer
The lower 4th layer of Lux of intensity of illumination 100~1000.Cooling system uses air conditioner for both cooling and heating, can be by the temperature control of glass culturing room
System is at 20~30 °C.A width of 60 centimetres of culturing rack, a height of 200 centimetres, 10 centimetres are the 4th layer from the ground, and layer is away from 60 centimetres, altogether
Four layers, culturing rack length can be depending on the size of culturing room.
3rd step：The blake bottle is placed on the 4th layer of culturing rack, temperature be 23 ~ 27 DEG C, intensity of illumination be 100~
Cultivated 60 ~ 90 days under conditions of 1000 Lux, make seed sprout to form protocorm, protocorm sends out roots obtains seedling with blade；
4th step：By the seedling on second and third layer of culturing rack, at 24 ~ 28 DEG C, intensity of illumination is 1000~3000 Lux's
Under the conditions of cultivate, obtain height for 3 ~ 5cm, blade quantity be 3~5, the quantity of coring is 3~5, the length of root is 1~3
Centimetre seedlings；
5th step：By the seedlings on first layer culturing rack, at 24 ~ 28 DEG C, intensity of illumination is 3000~5000 Lux bar
Cultivated under part, acquisition height is 5 ~ 7cm, blade quantity is more than 4, the quantity of coring is more than 5, a diameter of 3 milli of root
Rooted seedling more than rice；
6th step：The rooted seedling is taken out from blake bottle, cleans after being attached to the culture medium in the rooted seedling, uses permanganic acid
Potassium solution soaks, and cultivation is in matrix after the root for then drying rooted seedling to rooted seedling bleaches.
Preferably technical scheme is：By little Hua, all ages blue capsule volume fraction is alcohol-pickled 30 seconds of 70%, Ran Houyong
The mercuric chloride solution that mass fraction is 0.3 sterilizes 15 minutes, next uses aseptic water washing 5 times, with sterile under aseptic condition
Filter paper blots the moisture on the capsule surface；Then capsule is cut with aseptic operation knife, the seed in capsule is eluted to an appearance
Receive in the container for there are 200 milliliters of sterilized waters, seed suspension liquid is made.
Preferably technical scheme is：In the 6th step, with the liquor potassic permanganate immersion 4 that mass fraction is 0.1 ~ 0.32%
Minute.
As described above is only to explain the preferred embodiments of the invention, is not intended to tool to do any shape to the present invention
Limitation in formula, therefore all have any modification or change for making the relevant present invention under identical spirit, it should all wrap
Include the invention is intended to the category protected.
Claims (3)
- A kind of 1. little Hua all ages orchid species seedling aseptic seeding propagation methods, it is characterised in that：Comprise the following steps：The first step：By little Hua, all ages blue capsule with alcohol-pickled, is then sterilized with mercuric chloride solution, next with aseptic water washing, The moisture on the capsule surface is blotted with aseptic filter paper under aseptic condition；Then capsule is cut with aseptic operation knife, by capsule Seed in fruit is eluted to one and accommodated in the container of sterilized water, and seed suspension liquid is made, then with sterilized water by the seed Suspension is adjusted to the inoculation seed suspension liquid of 20 ~ 30 seed/milliliters of concentration；Second step：Under aseptic condition, the inoculation is moved to one with seed suspension liquid and accommodates once-seedling forming solid medium In orchid blake bottle, the seed amount in each orchid blake bottle is 40 ~ 60；Every liter of once-seedling forming culture medium contains Have 1 ~ 2g spend precious No. 1,1~2g spend precious No. 2,0.5~2g peptone, 40 ~ 80g mashed potatoes, 50 ~ 100g banana Mud, 10 ~ 20g apple butter, 500 ~ 1000mg activated carbon, 80 ~ 120mg inositol, 1.5 ~ 2.5mg glycine, 0.05 ~ 0.2mg thiamine hydrochloride, 0.4 ~ 0.8mg puridoxine hydrochloride, 0.4 ~ 0.8mg nicotinic acid, 0.05 ~ 0.1mg 6- benzyls are fast Purine, 0.2 ~ 2 mg methyl α-naphthyl acetate, 15~30g sucrose, 6~7g agar；The pH values of the once-seedling forming culture medium are 5.4- 5.6；3rd step：By the blake bottle temperature be 23 ~ 27 DEG C, intensity of illumination be 100~1000 Lux under conditions of culture 60 ~ 90 days, seed is set to sprout to form protocorm, protocorm sends out roots obtains seedling with blade；4th step：By the seedling at 24 ~ 28 DEG C, intensity of illumination is cultivated under conditions of being 1000~3000 Lux, obtains height For the seedlings that 3 ~ 5cm, blade quantity are 3~5, the quantity of coring is 3~5, the length of root is 1~3 centimetre；5th step：By the seedlings at 24 ~ 28 DEG C, intensity of illumination is cultivated under conditions of being 3000~5000 Lux, obtains height For 5 ~ 7cm, blade quantity be more than 4, the quantity of coring is more than 5, a diameter of more than 3 millimeters of rooted seedling of root；6th step：The rooted seedling is taken out from blake bottle, cleans after being attached to the culture medium in the rooted seedling, uses permanganic acid Potassium solution soaks, and cultivation is in matrix after the root for then drying rooted seedling to rooted seedling bleaches.
- 2. little Hua according to claim 1 all ages orchid species seedling aseptic seeding propagation methods, it is characterised in that：By little Hua all ages Blue capsule volume fraction is alcohol-pickled 20 ~ 40 seconds of 70%, is then sterilized with the mercuric chloride solution that mass fraction is 0.1 ~ 0.3% 15 ~ 20 minutes, next use aseptic water washing 4 ~ 5 times, the capsule surface is blotted with aseptic filter paper under aseptic condition Moisture；Then capsule is cut with aseptic operation knife, the seed in capsule is eluted to a container for accommodating 200 milliliters of sterilized waters It is interior, seed suspension liquid is made.
- 3. little Hua according to claim 1 all ages orchid species seedling aseptic seeding propagation methods, it is characterised in that：In the 6th step In, soaked 4 ~ 6 minutes with the liquor potassic permanganate that mass fraction is 0.1 ~ 0.3%.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108184671A (en) * | 2018-03-06 | 2018-06-22 | 山东寿光蔬菜种业集团有限公司 | A kind of method that tomato quickly breeds and improves hardening survival rate |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106386482A (en) * | 2016-08-31 | 2017-02-15 | 南京仙草堂生物科技有限公司 | Rapid propagation method of high-quality seedlings of Dendrobium officinale with natural light cultivation for one-step seedling formation |
| CN106472308A (en) * | 2016-09-30 | 2017-03-08 | 中国科学院华南植物园 | A kind of preservation of cymbidium seed and method for culturing seedlings |
-
2017
- 2017-09-29 CN CN201710903585.1A patent/CN107581067A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106386482A (en) * | 2016-08-31 | 2017-02-15 | 南京仙草堂生物科技有限公司 | Rapid propagation method of high-quality seedlings of Dendrobium officinale with natural light cultivation for one-step seedling formation |
| CN106472308A (en) * | 2016-09-30 | 2017-03-08 | 中国科学院华南植物园 | A kind of preservation of cymbidium seed and method for culturing seedlings |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108184671A (en) * | 2018-03-06 | 2018-06-22 | 山东寿光蔬菜种业集团有限公司 | A kind of method that tomato quickly breeds and improves hardening survival rate |
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