CN107568219B - Biocontrol liquid pesticide-fertilizer synergist and application method thereof - Google Patents
Biocontrol liquid pesticide-fertilizer synergist and application method thereof Download PDFInfo
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Abstract
The invention discloses a biocontrol liquid insecticide-fertilizer synergist and a use method thereof, wherein the synergist comprises the following components: a glycosyl surfactant, a polyoxyethylene ether surfactant, a biocontrol agent, a biostimulant and a stabilizer; the weight percentages of the components are as follows: 25-30% of glycosyl surfactant, 8-10% of polyoxyethylene ether surfactant, 5-7% of biocontrol microbial inoculum, 6-8% of biostimulant, 1-4% of stabilizer and the balance of water; the use steps are as follows: s1: diluting the biocontrol liquid pesticide-fertilizer synergist into a pesticide-fertilizer solution according to the dilution of 3000-fold and 5000-fold; s2: uniformly spraying the pesticide-fertilizer solution on the front and back surfaces of the blade; s3: finely adjusting the spray droplets, and controlling the flow rate to be 0.3L/min-0.5L/min; the speed of pesticide spraying and fertilizer spraying is accelerated and controlled to be 2m/s-2.5m/s, and water films for coating the whole blade are formed on the front surface and the back surface of the blade. The invention effectively solves the problems of low utilization rate of the pesticide fertilizer, high cost, environmental pollution and the like, and simultaneously has the functions of biological control and regulation.
Description
Technical Field
The invention relates to the technical field of pesticide-fertilizer synergists, in particular to a biocontrol liquid pesticide-fertilizer synergist and a using method thereof.
Background
Pesticides and fertilizers are important input substances for agricultural production, and promote agricultural development. With the development of agriculture in China, the pollution problem of pesticides and fertilizers is highlighted.
The pesticide for preventing and controlling diseases, pests and weeds is indispensable for ensuring the high quality and the high yield of agricultural products. But the pesticide spraying amount is large, the pesticide utilization rate is only 20% -30%, most of the pesticide is lost into soil or air, the pesticide effect is reduced, the pesticide residue, the pesticide application amount and the fertilizing frequency are increased, the environment is seriously polluted, and the pesticide resistance of plant diseases and insect pests and the pesticide use cost are improved. The method improves the utilization rate of the pesticide, and is an effective way for reducing the pesticide cost, reducing the pesticide residue and reducing the environmental pollution. Although the general utilization rate of the foliar fertilizer can reach 80%, the adhesion of the foliar fertilizer is small, the penetration rate of nutrients absorbed by leaves is low, and fertilizer liquid is easy to drip to soil or be leached by rainwater, so that economic waste and environmental pollution are caused. In addition, the foliar fertilizer is easy to dry on the surface of the leaf to form fertilizer spots, which affects nutrient absorption and beauty; the fertilizer efficiency of the foliar fertilizer is short, and the foliar fertilizer needs to be sprayed for many times, thus wasting manpower and the use cost of the foliar fertilizer. Therefore, the enhancement of the expansibility and the permeability of the foliar fertilizer and the utilization rate of the foliar fertilizer have important significance for promoting the nutrient absorption of plant leaves, reducing the use cost of the foliar fertilizer and relieving the environmental pollution.
Nowadays, researches on improving the utilization rate of pesticides and the spreading force and the penetrability of foliar fertilizers are more, and most of the researches are to add a surfactant or a penetrant into the pesticides and the fertilizers so as to reduce the surface tension of pesticide fertilizer liquid. In chinese patent document CN201610057006.1, the nonionic surfactant fatty alcohol polyoxyethylene ether is added to improve the drug effect and reduce the production cost. In chinese patent document CN201410098720.6, one or more surfactants selected from betaine, ammonium salt, amine oxide, palm kernel oleic acid diethanolamide, styrylphenol polyoxyethylene ether, and tallow amine polyoxyethylene ether are used to increase the permeability of the pesticide. Researches on Zhumintao and the like find that the tomato leaf fertilizer is added with surfactants such as Tween 80 and sodium dodecyl sulfate, so that the tomato can quickly absorb required nutrient elements, reduce flower and fruit dropping, and increase the yield and income. In chinese patent document CN201611171072.8, a surfactant is added to a foliar fertilizer to improve the fertilizer utilization rate, reduce the application amount of pesticides, and reduce the residual amount of plant pesticides.
However, the research on improving the utilization rate of the pesticide and the spreadability, the adhesive force and the penetrability of the foliar fertilizer is single, the research on combining the pesticide and the fertilizer is not carried out, and the performance of the surfactant or the penetrant is not exerted to the best. Therefore, research and development of the pesticide-fertilizer synergist which has the functions of improving the dressing property, the adhesive force and the permeability of pesticide-fertilizer, promoting the absorption of pesticide-fertilizer, improving the utilization rate of pesticide-fertilizer and biologically preventing and regulating has great significance for reducing pesticide-fertilizer residue, relieving environmental pollution and reducing agricultural production cost.
Disclosure of Invention
The invention aims to solve the technical problem of providing a biocontrol liquid pesticide-fertilizer synergist and a using method thereof, effectively solving the problems of low pesticide-fertilizer utilization rate, high cost, environmental pollution and the like, and simultaneously having biological control and regulation functions.
The invention adopts the technical scheme that a biocontrol liquid pesticide-fertilizer synergist is provided to solve the technical problems, wherein the biocontrol liquid pesticide-fertilizer synergist comprises the following components: sugar-based surfactant, polyoxyethylene ether surfactant, biocontrol agent, biostimulant and stabilizer.
The biocontrol liquid pesticide-fertilizer synergist comprises the following components in percentage by weight: 25-30% of glycosyl surfactant, 8-10% of polyoxyethylene ether surfactant, 5-7% of biocontrol microbial inoculum, 6-8% of biostimulant, 1-4% of stabilizer and the balance of water.
The biocontrol liquid pesticide-fertilizer synergist comprises the following components in percentage by weight: 30% of glycosyl surfactant, 9% of polyoxyethylene ether surfactant, 7% of biocontrol microbial inoculum, 7% of biostimulant, 2% of stabilizer and 45% of water.
In the biocontrol liquid insecticide-fertilizer synergist, the glycosyl surfactant and the polyoxyethylene ether surfactant are nonionic surfactants.
In the biocontrol liquid pesticide-fertilizer synergist, the glycosyl surfactant is dodecyl glucoside.
In the biocontrol liquid insecticide-fertilizer synergist, the polyoxyethylene ether surfactant is isomeric alcohol polyoxyethylene ether.
The biocontrol liquid pesticide-fertilizer synergist is a compound of fermentation liquids of paenibacillus polymyxa and trichoderma harzianum, and the compound volume ratio of the two is 1: 1.
In the biological control type liquid pesticide-fertilizer synergist, the biostimulant is a giant spirulina extract and contains auxin, cytokinin, gibberellin, abscisic acid, polypeptide and brassinolide.
In the biocontrol liquid insecticide-fertilizer synergist, the stabilizer is citric acid.
The invention also provides a use method of the biocontrol liquid pesticide-fertilizer synergist for solving the technical problems, wherein the use method comprises the following steps: s1: diluting the biocontrol liquid pesticide-fertilizer synergist into a pesticide-fertilizer solution according to the dilution of 3000-fold and 5000-fold; s2: uniformly spraying the pesticide-fertilizer solution on the front and back surfaces of the blade; s3: finely adjusting the spray droplets, and controlling the flow rate to be 0.3L/min-0.5L/min; the speed of pesticide spraying and fertilizer spraying is accelerated and controlled to be 2m/s-2.5m/s, and water films for coating the whole blade are formed on the front surface and the back surface of the blade.
Compared with the prior art, the method for producing the water-soluble carbon-based fertilizer by using the biomass has the following beneficial effects that: (1) the problems of low utilization rate of the pesticide fertilizer, high cost, environmental pollution and the like are effectively solved, and the biological control and regulation function is achieved, so that the pesticide fertilizer has multiple functions, is convenient to use, is safe and efficient; (2) after different nonionic surfactants are compounded, particularly the dodecyl glucoside and the isomeric alcohol polyoxyethylene ether are compounded, the performance is improved, particularly the surface tension of the dodecyl glucoside is greatly reduced, the dilution multiple is high, the dosage is small, and the effect is obvious; (3) the preferable non-ionic surfactant dodecyl glucoside belongs to a green surfactant, is easy to dissolve in water, has low surface tension, good stability, easy biodegradation, no influence of water hardness, good compatibility with other surfactants, and has the functions of sterilizing, improving enzyme activity and the like; (4) the isomeric alcohol polyoxyethylene ether is easy to dissolve in water, has strong permeability, adhesion and expansibility, resists hard water and has good stability; (5) the optimized paenibacillus polymyxa and trichoderma harzianum are compounded in a volume ratio of 1:1, so that the paenibacillus polymyxa and the trichoderma harzianum can better play a biological control function and have a synergistic effect; the paenibacillus polymyxa has good control effect on bacterial and fungal soil-borne diseases such as bacterial wilt, fusarium wilt, root rot, anthracnose and the like; the Trichoderma harzianum has better control effect on leaf diseases such as gray mold, powdery mildew, anthracnose, downy mildew, leaf mold and the like; (6) the giant spirulina extract contains auxin, cytokinin, gibberellin, abscisic acid, polypeptide, brassinolide and other biostimulants, and the auxin, the brassinolide, the cytokinin and the gibberellin have synergistic effect, so that the generation of nonspecific active factors in crops can be stimulated, the balance of endogenous hormones can be regulated, and the robust growth of the crops can be promoted.
Detailed Description
The invention is further illustrated by the following examples, which should not be construed as limiting the invention.
Example 1
The weight percentages of the components are as follows: 30% of glycosyl surfactant, 9% of polyoxyethylene ether surfactant, 7% of biocontrol microbial inoculum, 7% of biostimulant, 2% of stabilizer and 45% of water.
Example 2
The weight percentages of the components are as follows: 25% of glycosyl surfactant, 10% of polyoxyethylene ether surfactant, 5% of biocontrol microbial inoculum, 8% of biostimulant, 3% of stabilizer and 49% of water.
Example 3
The weight percentages of the components are as follows: 27% of glycosyl surfactant, 8% of polyoxyethylene ether surfactant, 6% of biocontrol microbial inoculum, 6% of biostimulant, 4% of stabilizer and 49% of water.
Example 4
The weight percentages of the components are as follows: 28% of glycosyl surfactant, 10% of polyoxyethylene ether surfactant, 7% of biocontrol microbial inoculum, 8% of biostimulant, 1% of stabilizer and 46% of water.
Example 5
The weight percentages of the components are as follows: 25% of glycosyl surfactant, 9% of polyoxyethylene ether surfactant, 7% of biocontrol microbial inoculum, 6% of biostimulant, 4% of stabilizer and 49% of water.
Example 6
The weight percentages of the components are as follows: 29% of glycosyl surfactant, 8% of polyoxyethylene ether surfactant, 5% of biocontrol microbial inoculum, 7% of biostimulant, 3% of stabilizer and 48% of water.
Example 7
The weight percentages of the components are as follows: 26% of glycosyl surfactant, 10% of polyoxyethylene ether surfactant, 6% of biocontrol microbial inoculum, 7% of biostimulant, 1% of stabilizer and 50% of water.
In all the above examples, the sugar-based surfactant is a nonionic surfactant, dodecyl glucoside; the polyoxyethylene ether surfactant is nonionic surfactant isomeric alcohol polyoxyethylene ether; the biocontrol microbial inoculum is a compound of fermentation broth of paenibacillus polymyxa and trichoderma harzianum, and the compound volume ratio of the two is 1: 1; the biological stimulin is giant spirulina extract and comprises 7.4 mug/L of auxin, 0.32 mug/L of cytokinin, 0.55 mug/L of gibberellin, 0.02 mug/L of abscisic acid, 4000 mug/L of polypeptide and 1.1 mug/L of brassinolide; the stabilizing agent is citric acid.
The concrete using method of the synergist provided by the invention is as follows: the pesticide-fertilizer synergist is used in combination with pesticides and fertilizers, is diluted by 5000 times of 3000 times and added into a pesticide-fertilizer solution, is uniformly sprayed on the front and back surfaces of the blade, and the spray droplets are finely adjusted, wherein the flow rate is controlled to be 0.3L/min-0.5L/min; the speed of pesticide spraying and fertilizer spraying is increased and controlled to be 2m/s-2.5m/s, and the spraying amount is preferably that the blades form a water film but do not drip water; the fertilizer can be used in the whole growth period of crops, and is specifically determined according to the spraying frequency of pesticide fertilizers. When the pesticide fertilizer synergist is used, the matters needing attention are as follows: (1) the product is prepared at present when in use, so that the influence on the use effect caused by long-term placement after dilution is avoided; (2) the dosage of the liquid medicine and the fertilizer liquid and the use frequency of the pesticide fertilizer are properly reduced, and the pesticide injury or the fertilizer injury caused by excessive absorption of the pesticide or the fertilizer is avoided; (3) the pesticide can be prevented from being mixed with strong acid and strong alkaline pesticide or fertilizer for use, so as to avoid influencing the effect.
The pesticide-fertilizer synergist is subjected to measurement of various evaluation indexes, and the specific measurement results are as follows:
TABLE 1 measurement results of evaluation indexes of examples
Note: in the above experiments, the fertilizer synergist of examples 1-7 was diluted 4000 times, and the same amount of water was used for CK instead of fertilizer synergist. From the measurement results, the pesticide-fertilizer synergist effectively reduces the surface tension of the solution on the blade, the complete spreading time of the liquid drop and increases the spreading radius of the liquid drop.
Test examples
In order to further verify that the pesticide-fertilizer synergist has the effects of improving the utilization rate of pesticides and fertilizers, biologically preventing and promoting the growth of crops and the like, a field pesticide effect test and a field fertilizer effect test are respectively carried out, and the specific implementation method and the result are as follows.
Field efficacy test example 1
The test crop is tomato, the control object is downy mildew, 3 treatments are set, and the treatment is carried out by the following steps: adding a pesticide-fertilizer synergist, diluting by 3000 times, adding into the fluorine bacterium and propamocarb solution, uniformly spraying, spraying for 1 time at the early stage of downy mildew, spraying for the second time after 7 days, spraying for the third time after 14 days, and spraying for three times; and (3) treatment 2: adding a pesticide-fertilizer synergist, diluting by 3000 times, adding into the fluorine bacterium and propamocarb solution, uniformly spraying, spraying for 1 time at the early stage of downy mildew, spraying for a second time after 7 days, and spraying for two times; and (3) treatment: the fluorine bacterium and propamocarb are sprayed for 1 time at the initial stage of downy mildew without adding a pesticide-fertilizer synergist, and the second pesticide is sprayed after 7 days and the third pesticide is sprayed after 14 days, and the spraying is totally carried out for three times. Each treatment was repeated three times, and the control effect was investigated 7 days after the second and 7 days after the third treatment, respectively, and the results are shown in the following table.
TABLE 2 comparison of the effect of the synergist for the pesticide and fertilizer on controlling downy mildew
From the above table 2, even if the application of one time is reduced, the control effect is still improved by 11.9% 7 days after the third application under the condition of adding the pesticide-fertilizer synergist, which shows that the pesticide-fertilizer synergist can improve the utilization rate of the pesticide, reduce the pesticide dosage and times, and simultaneously, beneficial microorganisms play a role in controlling fungal diseases.
Field efficacy test example 2
The test crop is cucumber, the control object is anthracnose, 3 treatments are set, and the treatment is as follows: adding a pesticide-fertilizer synergist, diluting by 4000 times, adding into the thiram wettable powder solution, uniformly spraying, spraying for 1 time at the early stage of anthracnose attack, spraying for the second time after 7 days, spraying for the third time after 14 days, and spraying for three times; and (3) treatment 2: adding a pesticide-fertilizer synergist, diluting by 4000 times, adding into the thiram wettable powder solution, uniformly spraying, spraying for 1 time at the early stage of anthracnose attack, spraying for a second time after 7 days, and spraying for two times; and (3) treatment: the Fumei wettable powder is sprayed for 1 time in the early stage of anthracnose, the second time after 7 days and the third time after 14 days without adding a pesticide-fertilizer synergist, and the spraying is totally carried out for three times. Each treatment was repeated three times, and the control effect was investigated 7 days after the second and 7 days after the third treatment, respectively, and the results are shown in the following table.
TABLE 3 comparison of the effect of synergist for pesticide and fertilizer for controlling anthrax
From the above table 3, even if the application of one time is reduced, the control effect is improved by 9.4% 7 days after the third application under the condition of adding the pesticide-fertilizer synergist, which shows that the pesticide-fertilizer synergist can improve the utilization rate of the pesticide, reduce the pesticide dosage and times, and simultaneously, beneficial microorganisms play a role in controlling fungal diseases.
Field fertilizer efficiency test example 1
The test crop is grapes, the test period is a fruit setting period and a harvesting period, 3 treatments are set, and the treatment is as follows: adding pesticide fertilizer synergist, diluting by 3000 times, adding into potassium dihydrogen phosphate solution, and spraying for 7-10 days for three times; and (3) treatment 2: adding pesticide fertilizer synergist, diluting by 3000 times, adding into potassium dihydrogen phosphate solution, and spraying for 7-10 days for two times; and (3) treatment: no synergist of pesticide and fertilizer is added, and monopotassium phosphate is sprayed on the leaf surfaces for 7-10 days for three times. Each treatment was repeated three times, the leaf thickness, the individual fruit weight and the yield of the grapes treated differently were measured, and the incidence of black rot of grapes was investigated, and the results are shown in the following table.
TABLE 4 Effect of different treatments on grape yield, Black rot
From the above table 4, under the condition of adding the pesticide-fertilizer synergist, the weight of a single grape is increased without reducing one-time fertilizer spraying and one-time fertilizer spraying is reduced compared with the treatment 3 (comparison), and meanwhile, the incidence of black rot is reduced by 59.3% and 44.7%, respectively, which shows that the pesticide-fertilizer synergist can effectively improve the fertilizer utilization rate, reduce the fertilizer dosage and frequency, reduce the cost, and simultaneously has the effect of biologically preventing and treating black rot.
Field fertilizer efficiency test example 2
The test crop is strawberry, the test period is fruit setting period-first harvesting period, 3 treatments are set, the treatment is 1: adding pesticide fertilizer synergist, diluting by 5000 times, adding into 15-6-35+ TE solution of high potassium fertilizer, and uniformly spraying for 8 days/time for three times; and (3) treatment 2: adding pesticide fertilizer synergist, diluting by 5000 times, adding into 15-6-35+ TE solution of high potassium fertilizer, and uniformly spraying for 8 days/time, and applying twice; and (3) treatment: no synergist of pesticide and fertilizer is added, and high potassium fertilizer 15-6-35+ TE is sprayed on the leaf surfaces for 8 days/time, and the application is carried out for three times. Each treatment is repeated three times, the relative content of chlorophyll, the weight of a single fruit and the yield of a single crop of strawberries treated differently are respectively measured, meanwhile, the morbidity of strawberry blight is investigated, and the results are shown in the following table.
TABLE 5 Effect of different treatments on strawberry growth, yield and blight
As shown in the above table 5, under the condition of adding the pesticide-fertilizer synergist, the relative content of chlorophyll (SPAD value), the single fruit weight and the single crop yield of the strawberries are improved compared with those of the strawberry fertilizer of the treatment 3 (comparison), and the morbidity of the blight is reduced by 36.8 percent and 26.4 percent respectively, so that the pesticide-fertilizer synergist effectively improves the fertilizer utilization rate, reduces the fertilizer dosage and frequency, reduces the cost and has the effect of biologically preventing and treating the blight.
Although the present invention has been described with reference to the preferred embodiments, it is not intended to be limited thereto. The present invention is not limited to the above-described embodiments, and modifications and improvements can be made by those skilled in the art without departing from the spirit and scope of the present invention, but should be construed as being within the scope of the present invention.
Claims (4)
1. A biocontrol liquid insecticide-fertilizer synergist is characterized by comprising the following components: a glycosyl surfactant, a polyoxyethylene ether surfactant, a biocontrol agent, a biostimulant and a stabilizer;
the weight percentages of the components are as follows: 25-30% of glycosyl surfactant, 8-10% of polyoxyethylene ether surfactant, 5-7% of biocontrol microbial inoculum, 6-8% of biostimulant, 1-4% of stabilizer and the balance of water;
the biocontrol microbial inoculum is a compound of fermentation broth of paenibacillus polymyxa and trichoderma harzianum, and the compound volume ratio of the two is 1: 1;
the biological stimulin is giant spirulina extract, and contains auxin, cytokinin, gibberellin, abscisic acid, polypeptide and brassinolide;
the glycosyl surfactant and the polyoxyethylene ether surfactant are both nonionic surfactants;
the glycosyl surfactant is dodecyl glucoside;
the polyoxyethylene ether surfactant is isomeric alcohol polyoxyethylene ether.
2. The biocontrol liquid insecticide-fertilizer synergist of claim 1, wherein the weight percentage of each component is as follows: 30% of glycosyl surfactant, 9% of polyoxyethylene ether surfactant, 7% of biocontrol microbial inoculum, 7% of biostimulant, 2% of stabilizer and 45% of water.
3. The biocontrol liquid insecticide-fertilizer synergist of claim 1 or 2, wherein said stabilizer is citric acid.
4. The use method of the biocontrol liquid insecticide-fertilizer synergist disclosed by any one of claims 1 to 3 is characterized by comprising the following steps of:
s1: diluting the biocontrol liquid pesticide-fertilizer synergist into a pesticide-fertilizer solution according to the dilution of 3000-fold and 5000-fold;
s2: uniformly spraying the pesticide-fertilizer solution on the front and back surfaces of the blade;
s3: finely adjusting the spray droplets, and controlling the flow rate to be 0.3L/min-0.5L/min; the speed of pesticide spraying and fertilizer spraying is accelerated and controlled to be 2m/s-2.5m/s, and water films for coating the whole blade are formed on the front surface and the back surface of the blade.
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| CN108059566A (en) * | 2018-01-18 | 2018-05-22 | 迪斯科科技集团（宜昌）有限公司 | A kind of poly spirulina peptide biostimulant |
| EP3793364A1 (en) * | 2018-05-17 | 2021-03-24 | Bayer CropScience LP | Stabilized fungicidal composition |
| CN108990684B (en) * | 2018-09-04 | 2021-01-12 | 山东省果树研究所 | Grape cultivation method suitable for non-buried cold-proof area |
| CN110506760A (en) * | 2019-08-14 | 2019-11-29 | 山东劲脉植物细胞信息技术有限公司 | A kind of plant biological composition, preparation and its method of administration |
| CN111493067B (en) * | 2020-04-15 | 2021-11-09 | 上海抚佳精细化工有限公司 | Pesticide combination synergist and application thereof |
| CN111621020B (en) * | 2020-05-09 | 2021-09-21 | 华南理工大学 | Giant surfactant and preparation method thereof |
| CN112142515A (en) * | 2020-09-27 | 2020-12-29 | 陕西科技大学 | Polypeptide chelated trace element foliar fertilizer as well as preparation method and use method thereof |
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| CN105580843A (en) * | 2015-12-19 | 2016-05-18 | 佛山市艳晖生物科技有限公司 | Brassinolide containing spore pesticide fertilizer and preparation method thereof |
| CN106135291A (en) * | 2016-07-14 | 2016-11-23 | 解晓燕 | A kind of prevent and treat soil-borne disease, improve crop cold resistance compositions |
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Address after: 200333 room 306, 3 building, 879 Zhongjiang Road, Putuo District, Shanghai. Applicant after: Shanghai Yongtong ecological engineering Limited by Share Ltd Address before: 200333 room 306, 3 building, 879 Zhongjiang Road, Putuo District, Shanghai. Applicant before: Shanghai Yongtong Chemical Co., Ltd. |
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| GR01 | Patent grant | ||
| GR01 | Patent grant |