CN107550854A

CN107550854A - A kind of Venenum Bufonis Injection and preparation method thereof

Info

Publication number: CN107550854A
Application number: CN201710647693.7A
Authority: CN
Inventors: 黎景芬; 韩志波
Original assignee: Individual
Current assignee: Individual
Priority date: 2017-08-01
Filing date: 2017-08-01
Publication date: 2018-01-09

Abstract

The invention discloses a kind of Venenum Bufonis Injection, is formed by following components mixed preparing in parts by weight：Include 2.5 grams of toad cake extract in every 1000 milliliters of Venenum Bufonis Injections, Tween 80,10 milliliters, anhydrous sodium sulfite 2g, 20 milliliters of phenmethylol, appropriate water for injection.A kind of Venenum Bufonis Injection that the present invention is to provide, treated available for digestive system cancer, liver cancer etc..

Description

A kind of Venenum Bufonis Injection and preparation method thereof

Technical field

The present invention relates to pharmaceutical technology field, more particularly to a kind of Venenum Bufonis Injection and preparation method thereof.

Background technology

Dried venom of toads alias is also known as frog slurry, and frog is crisp, dried venom of toads eyebrow fat.Chinese Pharmacopoeia version in 2000 carries：Dried venom of toads pungent-warm, it is poisonous, The effect of tool removing toxic substances, inducing resuscitation of having one's ideas straightened out and analgesic.The dried venom of toads is the ear rear gland and skin gland secretion thing by Bufonidae amphibians faunal Process from strand, be traditional Chinese medicine.The bioactive substance such as steroidal toad clothing-medicinal thing, alkaloid contained by the dried venom of toads has solution Poison, the multiple functions such as ease pain, have one's ideas straightened out, be antitumor and being widely used.

The content of the invention

To solve above mentioned problem present in prior art and actual conditions, the invention provides a kind of Venenum Bufonis Injection, It is to be formed by following components mixed preparing in parts by weight：Toad cake extract is included in every 1000 milliliters of Venenum Bufonis Injections 2.5 grams, Tween 80,10 milliliters, anhydrous sodium sulfite 2g, 20 milliliters of phenmethylol, appropriate water for injection.

Another aspect of the present invention, there is provided a kind of preparation method of foregoing Venenum Bufonis Injection, carry out as follows：

Dried venom of toads 10g is taken, adds a small amount of water for injection to be fully ground, adds water to 400 milliliters, adds 1120 milliliters of 95% ethanol, puts Refrigerator 12h, after taking-up plus sodium chloride 10g makes protein coagulation；Then filtered with filter paper, filtrate recycling ethanol simultaneously concentrates in water-bath To paste, 100 milliliters of dissolvings of methanol, filtering, filtrate recovery or removing methanol are added, then adds 60 milliliters of dissolution filters of methanol, Filtrate eliminates methanol in water-bath, obtains paste alcohol extracting thing；

Above-mentioned paste alcohol extracting thing is weighed, Tween 80 is added and fully stirs evenly, put and hydrotropy is heated in water-bath, adds appropriate note Penetrate and use water and phenmethylol, anhydrous sodium sulfite, adjust pH value that it is thick to add the talcum powder that mass ratio is 5% to 7.55 with 10% hydrochloric acid Filter, then by activated carbon filtering layer refined filtration, until it is clear and bright, add and add to the full amount of water for injection, embedding, sterilizing, you can obtain.

Preferably, step is taken in the dried venom of toads, and the dried venom of toads used is the milk secreted by toad ear rear gland and glandula integumentaria through drying The faint yellow tablet of gained.

Preferably, soil is washed with water in the toad of seizure by annual spring and autumn, after drying, with ox horn, metal or bamboo Scraper made of piece scrapes the white juice of toad ear gland and cutaneous gland, makes in inflow magnetic basin or tank, puts toad after having scraped In backwater, in case next time use again, by the secretion of scraping with copper sieve 80 mesh filter off soil after, be applied on glass plate dry it is i.e. in blocks It is crisp.

Beneficial effects of the present invention：

A kind of Venenum Bufonis Injection that the present invention is to provide, treated available for digestive system cancer, liver cancer etc..

Embodiment

With reference to preferred embodiment, to according to embodiment provided by the invention, details are as follows：

Embodiment 1

A kind of preparation method of Venenum Bufonis Injection, is carried out as follows：

Dried venom of toads 10g is taken, adds a small amount of water for injection to be fully ground, adds water to 400 milliliters, adds 1120 milliliters of 95% ethanol (to make 70%) alcohol content is up to refrigerator 12h, is put, after taking-up plus sodium chloride 10g makes protein coagulation；Then (sediment drying is filtered with filter paper Preservation is separately dealt with), filtrate recycling ethanol is simultaneously concentrated into paste in water-bath, adds 100 milliliters of dissolvings of methanol, filters, filter Liquid reclaims or removed methanol, then adds 60 milliliters of dissolution filters of methanol, and filtrate eliminates methanol in water-bath, obtains paste alcohol extracting thing (about 4g).

Recipe quantity extract is weighed, Tween 80 is added and fully stirs evenly, put and hydrotropy (part is insoluble) is heated in water-bath, is added suitable Water for injection and phenmethylol, anhydrous sodium sulfite are measured, adjusts pH value to add the talcum that mass ratio is 5% to 7.55 with 10% hydrochloric acid Powder coarse filtration, then by activated carbon filtering layer refined filtration, until it is clear and bright, add and add to the full amount of water for injection, embedding, sterilizing, you can obtain.

It should be noted that dried venom of toads system used takes toad (toad) ear rear gland and milk secreted by glandula integumentaria through dry The faint yellow tablet of dry gained (piece is crisp).

Further, the method for gathering and processing of the dried venom of toads is that soil is washed with water in the toad of seizure by annual spring and autumn, is dried Afterwards, the scraper made of ox horn, metal or bamboo chip scrapes the white juice of toad ear gland and cutaneous gland, make inflow magnetic basin or (avoid in tank with ironware), put back to toad in water after having scraped, in case again with (toad can about take the fresh slurries of 4-5g), will scrape next time After the secretion taken sieves (80 mesh) elimination soil with copper, it is applied on glass plate and dries shortcake i.e. in blocks, tear and save backup.

Pleasing to the eye or suction is prevented in addition, the dried venom of toads is hypertoxic, during operation.

Embodiment 2

Take dried venom of toads 5g, add a small amount of phosphate buffer (PH=8) grind bubble, add pancreatin 1g, under 37 degrees Celsius after Continuous grinding, and continuous plus buffer solution adds anhydrous sodium sulfite 2g, 20 milliliters of phenmethylol, injects water to 1000 to 500 milliliters Milliliter, puts 37-40 degrees Celsius of case inside holding 24h of hatching, and takes out, and autoclaving (110 degrees Celsius 30 minutes), matter is added after cooling The talcum powder initial filter that amount ratio is 5%, then with 0.1% activated carbon refined filtration to clear and bright, filtering, embedding, sterilizing, you can obtain, per in the least Rise and contain 5 milligrams of the dried venom of toads.

This product is the sterile water solution of dried venom of toads alcohol extracting thing, and every milliliter contains 2.5 grams of alcohol extracting thing, 1 milliliter every or 2 millis Rise.

In terms of usage, daily 1-2 time, each 1-2 milliliters, patient can and meanwhile fiting chemical or radiotherapy, it can also be used to Intramuscular injection.

Finally it should be noted that：The preferred embodiments of the present invention are the foregoing is only, are not intended to limit the invention, Although the present invention is described in detail with reference to the foregoing embodiments, for those skilled in the art, it still may be used To be modified to the technical scheme described in foregoing embodiments, or equivalent substitution is carried out to which part technical characteristic. Within the spirit and principles of the invention, any modification, equivalent substitution and improvements made etc., it should be included in the present invention's Within protection domain.

Claims

1. a kind of Venenum Bufonis Injection, it is characterised in that formed by following components mixed preparing in parts by weight：Every 1000 milliliters 2.5 grams of toad cake extract is included in the Venenum Bufonis Injection, Tween 80,10 milliliters, anhydrous sodium sulfite 2g, phenmethylol 20 is in the least Rise, appropriate water for injection.

2. a kind of preparation method of Venenum Bufonis Injection according to claim 1, it is characterised in that carry out as follows：

Dried venom of toads 10g is taken, adds a small amount of water for injection to be fully ground, adds water to 400 milliliters, adds 1120 milliliters of 95% ethanol, puts refrigerator 12h, after taking-up plus sodium chloride 10g makes protein coagulation；Then filtered with filter paper, filtrate recycling ethanol is simultaneously concentrated into thick in water-bath Paste, 100 milliliters of dissolvings of methanol, filtering, filtrate recovery or removing methanol are added, then add 60 milliliters of dissolution filters of methanol, filtrate Methanol is eliminated in water-bath, obtains paste alcohol extracting thing；

Above-mentioned paste alcohol extracting thing is weighed, Tween 80 is added and fully stirs evenly, put and hydrotropy is heated in water-bath, add appropriate injection Water and phenmethylol, anhydrous sodium sulfite, with 10% hydrochloric acid tune pH value to 7.55, the talcum powder coarse filtration that mass ratio is 5% is added, then By activated carbon filtering layer refined filtration, until it is clear and bright, add and add to the full amount of water for injection, embedding, sterilizing, you can obtain.

3. the preparation method of Venenum Bufonis Injection according to claim 2, it is characterised in that step is taken in the dried venom of toads, toad used Shortcake is faint yellow tablet of the milk secreted by toad ear rear gland and glandula integumentaria through dry gained.

4. the preparation method of Venenum Bufonis Injection according to claim 2, it is characterised in that the step dried venom of toads gathers and processes method For, soil is washed with water in the toad of seizure by annual spring and autumn, after drying, the scraper made of ox horn, metal or bamboo chip The white juice of toad ear gland and cutaneous gland is scraped, makes in inflow magnetic basin or tank, puts back to toad in water after having scraped, in case under It is secondary to use again, after the secretion of scraping is sieved into 80 mesh elimination soil with copper, it is applied on glass plate and dries shortcake i.e. in blocks.