CN107549486A - A kind of raising sow fertility rate, the composition containing probiotics and composite extract of piglet litter weight at birth - Google Patents

Abstract

The invention belongs to technical field of agricultural breeding, more particularly to a kind of composition containing probiotics and composite extract for improving sow fertility rate, piglet litter weight at birth, including bacillus subtilis, lactobacillus acidophilus, in addition to excrement enterobacteria mycoplasma extract, the Radix Astragali, Radix Codonopsis, Radix Angelicae Sinensis, honeysuckle and Shorthorned Epimedium P.E.The present invention has advantages below compared with prior art：By being extracted to excrement coccobacillus mycoplasma in the present invention, mixed with probiotics and Chinese medical extract, obtain said composition, can aid in digestion, the absorption for further improving the in-pig middle and later periods to nutriment；One is built in its enteron aisle and is beneficial to beneficial bacteria, the micro-ecological environment of resist the disease, is strengthened the ability of resist the disease, is reduced the generation of endogenous toxic material, reduce the infectious rate to offspring；Fat content, raising number born of sow, raising birth weight, extension reproductive life, the generation of reduction sow metria, vaginitis in 24 hours breasts after sow produces can be improved by adding said composition.

Description

It is a kind of improve sow fertility rate, piglet litter weight at birth contains probiotics and compound collecting The composition of thing

Technical field

The invention belongs to technical field of agricultural breeding, and in particular to a kind of to improve sow fertility rate, piglet litter weight at birth Composition containing probiotics and composite extract.

Background technology

Sow reproductive performance is the key point of pig farm production and management, and influence factor is numerous, be can be summarized as in vivo and in vitro Two broad aspects, the nutrition of the nutrition of sow gestation phase, particularly latter half of gestation has a great influence to fetal weight change, if nutrition Intake can not meet embryo growth and development requirement, and sow will appear from nutrition negative balance, and piglet birth weight and weaning weight are smaller, existing In technology, generally in sow gestation later stage sow feeding High-energy high-protein feed, to ensure development of fetus, Farrowing and lactation The nutritional need of phase, but anabolism is strengthened after sow enters gestation, and the utilization rate of nutriment is improved, is not suitable for raising Feed energy very high feed, carry out limit and raise, lower the content of the energy in feed, the death rate of embryo can be reduced, but accordingly , farrowing rate and piglet birth weight but also it is corresponding decline, therefore sow gestation later stage feed should further be studied.

The content of the invention

The purpose of the present invention be for it is existing the problem of, there is provided it is a kind of to improve sow fertility rate, piglet litter weight at birth Composition containing probiotics and composite extract.

The present invention is achieved by the following technical solutions：It is a kind of improve sow fertility rate, piglet litter weight at birth containing benefit Raw bacterium and the composition of composite extract, including bacillus subtilis, lactobacillus acidophilus, in addition to the extraction of excrement enterobacteria mycoplasma Thing, the Radix Astragali, Radix Codonopsis, Radix Angelicae Sinensis, honeysuckle and Shorthorned Epimedium P.E；

Wherein, excrement enterobacteria mycoplasma extract is made by following methods：

（1）Seed culture：With 0.5% inoculum concentration, inclined-plane seed is seeded in seed culture medium, in 37 DEG C of 10h that rest, i.e., Obtain seed liquor；

Wherein, seed culture medium includes tryptone 18g/L, dusty yeast 5g/L, glucose 3g/L, peptone 5g/L, lactose 2.2g/L, calcium carbonate 3.3g/L, corn juice 100g/L, carrot juice 80g/L, pH value 6.5 before sterilizing；

（2）Seed liquor is fermented：With 3.5% inoculum concentration, seed liquor is seeded in fermentation medium, at 37 DEG C, concussion and cultivate is extremely Calcium carbonate disappears；

Wherein, seed culture medium includes tryptone 26g/L, dusty yeast 2g/L, glucose 3.6g/L, peptone 4.5g/L, breast Sugared 1.5g/L, calcium carbonate 3.3g/L, corn juice 80g/L, carrot juice 60g/L, pH value 6.8 before sterilizing；

（3）Fermentation flask fermenting and producing culture：Under conditions of temperature is 36 DEG C, pH value is 6.27, cultivates 12-15 days, obtain excrement Enterobacteria mycoplasma；

（4）The ethanol solution refluxing extraction 2 hours for being 40-80% with concentration of volume percent by mycoplasma, then at 3600 revs/min Centrifuged 12 minutes under the conditions of clock, filter, obtain extract solution；Then revolving to without alcohol, adds elutriation to go out heavy under conditions of 65-75 DEG C Starch, then centrifuged 8 minutes under conditions of 3000 revs/min, sediment is dried under conditions of -30 DEG C of -50-, produced Mycoplasma extract.

As further improvement of these options, the sediment is dried under conditions of -40 DEG C.

As further improvement of these options, the weight ratio of the ethanol solution and mycoplasma is 10:3.

As further improvement of these options, the viable count of the bacillus subtilis and lactobacillus acidophilus is 200-400 hundred million/gram, water content is 6.3%.

As further improvement of these options, the preparation method of the Shorthorned Epimedium P.E is：Barrenwort is dried Crush, cross 200 mesh sieves；By barrenwort xeraphium in refluxing extraction kettle, control temperature to be extracted 6 hours for 65 DEG C, centrifuge Obtain extract solution；3 times repeatedly, No. 3 extract solutions are merged, is concentrated in vacuo in mixed extract, obtains Shorthorned Epimedium P.E.

As further improvement of these options, the solvent in the refluxing extraction kettle is methanol, ether or acetic acid second Any one in ester.

As further improvement of these options, include the raw material of following parts by weight：It is bacillus subtilis 1-2 parts, thermophilic Lactobacillus lactis 1-2 parts, in addition to excrement enterobacteria mycoplasma extract 0.8-1.5 parts, Radix Astragali 16-20 parts, Radix Codonopsis 16-20 parts, Radix Angelicae Sinensis 8-15 parts, honeysuckle 15-20 parts and Shorthorned Epimedium P.E 6-10 parts.

As further improvement of these options, its preparation method comprises the following steps：

（1）After the Radix Astragali, Radix Codonopsis, Radix Angelicae Sinensis are processed respectively, the crushed material that particle diameter is 1-5mm is ground into, mixing, is added equivalent to it The water that 10 times of weight, after infusion 4 hours, filtering, obtains filtrate, is concentrated and dried dusts under cryogenic, obtaining water content is 8-10% mixed powder；

（2）By mixed powder successively with Shorthorned Epimedium P.E, excrement enterobacteria mycoplasma extract and bacillus subtilis, acidophilus breast bar Bacterium, which mixes, to be produced.

Composition is used for the middle and later periods of in-pig in the present invention（From 80 days）, before production, the composition is in day Addition in grain is the 1-2% of its weight, and the daily ration is sow later stage special feed, and its later stage special feed is except necessary Outside nutriment and trace element, in addition to fatty powder 3-5%, cattle and sheep fat 2-4%, fish oil 2%.

The excrement enterobacteria is purchased from Chinese industrial Culture Collection station.

The present invention has advantages below compared with prior art：By being extracted to excrement coccobacillus mycoplasma in the present invention, with Probiotics and Chinese medical extract mixing, obtain said composition, can aid in and further improve the in-pig middle and later periods to nutrition The digestion of material, absorb；One is built in its enteron aisle and is beneficial to beneficial bacteria, the micro-ecological environment of resist the disease, enhancing resistance The ability of disease, the generation of endogenous toxic material is reduced, reduce the infectious rate to offspring；Addition said composition can be improved 24 after sow production Fat content in hour breast, improves number born of sow, improves birth weight and vigor, extends the reproductive life, reduction sow postpartum The generation of cervicitis, vaginitis.

Embodiment

Embodiment 1

A kind of raising sow fertility rate, the composition containing probiotics and composite extract of piglet litter weight at birth, including withered grass bud Spore bacillus, lactobacillus acidophilus, in addition to excrement enterobacteria mycoplasma extract, the Radix Astragali, Radix Codonopsis, Radix Angelicae Sinensis, honeysuckle and barrenwort extraction Thing；

Wherein, excrement enterobacteria mycoplasma extract is made by following methods：

（1）Seed culture：With 0.5% inoculum concentration, inclined-plane seed is seeded in seed culture medium, in 37 DEG C of 10h that rest, i.e., Obtain seed liquor；

Wherein, seed culture medium includes tryptone 18g/L, dusty yeast 5g/L, glucose 3g/L, peptone 5g/L, lactose 2.2g/L, calcium carbonate 3.3g/L, corn juice 100g/L, carrot juice 80g/L, pH value 6.5 before sterilizing；

（2）Seed liquor is fermented：With 3.5% inoculum concentration, seed liquor is seeded in fermentation medium, at 37 DEG C, concussion and cultivate is extremely Calcium carbonate disappears；

Wherein, seed culture medium includes tryptone 26g/L, dusty yeast 2g/L, glucose 3.6g/L, peptone 4.5g/L, breast Sugared 1.5g/L, calcium carbonate 3.3g/L, corn juice 80g/L, carrot juice 60g/L, pH value 6.8 before sterilizing；

（3）Fermentation flask fermenting and producing culture：Under conditions of temperature is 36 DEG C, pH value is 6.27, cultivates 12-15 days, obtain excrement Enterobacteria mycoplasma；

（4）By the ethanol solution refluxing extraction 2 hours that mycoplasma concentration of volume percent is 60%, then in 3600 revs/min of bars Centrifuged 12 minutes under part, filter, obtain extract solution；Then revolving to without alcohol, adds elutriation to go out sediment, so under conditions of 70 DEG C Centrifuged 8 minutes under conditions of 3000 revs/min afterwards, sediment is dried under conditions of -30 DEG C of -50-, mycoplasma is produced and carries Take thing.

Wherein, the weight of the ethanol solution and mycoplasma ratio is 10:3；The bacillus subtilis and lactobacillus acidophilus Viable count be 200-400 hundred million/gram, water content is 6.3%.

Wherein, the preparation method of the Shorthorned Epimedium P.E is：By barrenwort drying and crushing, 200 mesh sieves are crossed；By barrenwort Xeraphium extracts 6 hours using ethyl acetate in the refluxing extraction kettle of solvent, to control temperature to be 65 DEG C, is centrifugally separating to obtain and carries Take liquid；3 times repeatedly, No. 3 extract solutions are merged, is concentrated in vacuo in mixed extract, obtains Shorthorned Epimedium P.E.

Wherein, the raw material of following parts by weight is included：2 parts of bacillus subtilis, 1 part of lactobacillus acidophilus, in addition to excrement intestines bar 8 parts of 1.2 parts of bacterium mycoplasma extract, 18 parts of the Radix Astragali, 18 parts of Radix Codonopsis, 12 parts of Radix Angelicae Sinensis, 18 parts of honeysuckle and Shorthorned Epimedium P.E；

Its preparation method comprises the following steps：

（1）After the Radix Astragali, Radix Codonopsis, Radix Angelicae Sinensis are processed respectively, the crushed material that particle diameter is 1-5mm is ground into, mixing, is added equivalent to it The water that 10 times of weight, after infusion 4 hours, filtering, obtains filtrate, is concentrated and dried dusts under cryogenic, obtaining water content is 8-10% mixed powder；

（2）By mixed powder successively with Shorthorned Epimedium P.E, excrement enterobacteria mycoplasma extract and bacillus subtilis, acidophilus breast bar Bacterium, which mixes, to be produced.

Composition is used for the middle and later periods of in-pig in the present invention（From 80 days）, before production, the composition is in day Addition in grain is the 1-2% of its weight, and the daily ration is sow later stage special feed, and its later stage special feed is except necessary Outside nutriment and trace element, in addition to fatty powder 3-5%, cattle and sheep fat 2-4%, fish oil 2%.

Setting experiment to take, 32 breeding times are close, parity is identical, and body weight is substantially close, and the length being in a good state of health is white × Yorkshire is divided into two treatment groups through sow stochastic averagina, each handles 4 repetitions, and each to repeat 4, wherein treatment group 1 is Control group, feed basal diet；Treatment group 2 is test group, feeding basal diet+equivalent in the embodiment 1 of basal diet 2% Sow is driven in positioning rail raising before starting, tests and terminate from sow gestation 80 days to weaned piglet by the composition of preparation, experiment, Its production performance is detected, and records related data, obtains below table：

Table 1

Project	Experimental group	Control group
			Sow feed intake（kg）	159.8	162.5
Piglet is come into being counterpoise（kg）	1.28	1.22
			Weaned piglet counterpoise（kg）	7.83	6.42
Average number born alive（Head）	9.05	8.82
			Average weaning piglet（Head）	8.72	7.46
Piglet survival ratio（%）	96.35	84.58

Can be seen that composition in the present invention by data in table 1 makes in-pig middle and later periods feed intake essentially identical, but it is young Pig birth weight and survival rate have certain raising, and production performance synthesis improves.

Claims (9)

1. a kind of raising sow fertility rate, the composition containing probiotics and composite extract of piglet litter weight at birth, its feature exist In, including bacillus subtilis, lactobacillus acidophilus, in addition to excrement enterobacteria mycoplasma extract, the Radix Astragali, Radix Codonopsis, Radix Angelicae Sinensis, honeysuckle And Shorthorned Epimedium P.E；

Wherein, excrement enterobacteria mycoplasma extract is made by following methods：

（1）Seed culture：With 0.5% inoculum concentration, inclined-plane seed is seeded in seed culture medium, in 37 DEG C of 10h that rest, i.e., Obtain seed liquor；

Wherein, seed culture medium includes tryptone 18g/L, dusty yeast 5g/L, glucose 3g/L, peptone 5g/L, lactose 2.2g/L, calcium carbonate 3.3g/L, corn juice 100g/L, carrot juice 80g/L, pH value 6.5 before sterilizing；

（2）Seed liquor is fermented：With 3.5% inoculum concentration, seed liquor is seeded in fermentation medium, at 37 DEG C, concussion and cultivate is extremely Calcium carbonate disappears；

Wherein, seed culture medium includes tryptone 26g/L, dusty yeast 2g/L, glucose 3.6g/L, peptone 4.5g/L, breast Sugared 1.5g/L, calcium carbonate 3.3g/L, corn juice 80g/L, carrot juice 60g/L, pH value 6.8 before sterilizing；

（3）Fermentation flask fermenting and producing culture：Under conditions of temperature is 36 DEG C, pH value is 6.27, cultivates 12-15 days, obtain excrement Enterobacteria mycoplasma；

（4）The ethanol solution refluxing extraction 2 hours for being 40-80% with concentration of volume percent by mycoplasma, then at 3600 revs/min Centrifuged 12 minutes under the conditions of clock, filter, obtain extract solution；Then revolving to without alcohol, adds elutriation to go out heavy under conditions of 65-75 DEG C Starch, then centrifuged 8 minutes under conditions of 3000 revs/min, sediment is dried under conditions of -30 DEG C of -50-, produced Mycoplasma extract.

2. as claimed in claim 1 it is a kind of improve sow fertility rate, piglet litter weight at birth containing probiotics and composite extract Composition, it is characterised in that the sediment is dried under conditions of -40 DEG C.

3. as claimed in claim 1 it is a kind of improve sow fertility rate, piglet litter weight at birth containing probiotics and composite extract Composition, it is characterised in that the weight of the ethanol solution and mycoplasma ratio is 10:3.

4. as claimed in claim 1 it is a kind of improve sow fertility rate, piglet litter weight at birth containing probiotics and composite extract Composition, it is characterised in that the viable count of the bacillus subtilis and lactobacillus acidophilus be 200-400 hundred million/gram, water content It is 6.3%.

5. as claimed in claim 1 it is a kind of improve sow fertility rate, piglet litter weight at birth containing probiotics and composite extract Composition, it is characterised in that the preparation method of the Shorthorned Epimedium P.E is：By barrenwort drying and crushing, 200 mesh sieves are crossed；Will Barrenwort xeraphium controls temperature to be extracted 6 hours for 65 DEG C, is centrifugally separating to obtain extract solution in refluxing extraction kettle；Repeatedly 3 It is secondary, No. 3 extract solutions are merged, is concentrated in vacuo in mixed extract, obtains Shorthorned Epimedium P.E.

6. as claimed in claim 5 it is a kind of improve sow fertility rate, piglet litter weight at birth containing probiotics and composite extract Composition, it is characterised in that the solvent in the refluxing extraction kettle is any one in methanol, ether or ethyl acetate.

7. as claimed in claim 1 it is a kind of improve sow fertility rate, piglet litter weight at birth containing probiotics and composite extract Composition, it is characterised in that include the raw material of following parts by weight：Bacillus subtilis 1-2 parts, lactobacillus acidophilus 1-2 parts, also wrap Include excrement enterobacteria mycoplasma extract 0.8-1.5 parts, Radix Astragali 16-20 parts, Radix Codonopsis 16-20 parts, Radix Angelicae Sinensis 8-15 parts, honeysuckle 15-20 Part and Shorthorned Epimedium P.E 6-10 parts.

8. as claimed in claim 1 it is a kind of improve sow fertility rate, piglet litter weight at birth containing probiotics and composite extract Composition, it is characterised in that its preparation method comprises the following steps：

（1）After the Radix Astragali, Radix Codonopsis, Radix Angelicae Sinensis are processed respectively, the crushed material that particle diameter is 1-5mm is ground into, mixing, is added equivalent to it The water that 10 times of weight, after infusion 4 hours, filtering, obtains filtrate, is concentrated and dried dusts under cryogenic, obtaining water content is 8-10% mixed powder；

（2）By mixed powder successively with Shorthorned Epimedium P.E, excrement enterobacteria mycoplasma extract and bacillus subtilis, acidophilus breast bar Bacterium, which mixes, to be produced.

9. as any one of claim 1-8 it is a kind of improve sow fertility rate, piglet litter weight at birth containing probiotics with it is multiple Close the composition of extract, it is characterised in that addition of the composition in daily ration is the 1-2% of its weight.