CN107541472A - A kind of new microbe agent and preparation method thereof - Google Patents
A kind of new microbe agent and preparation method thereof Download PDFInfo
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Abstract
A kind of new microbe agent of the present invention makees mother liquor using natural organic protein, chelate bacillus licheniformis, bacillus subtilis and various trace elements, through kinds of processes flow, deep processing refines out a new generation and contains 2,800,000,000/ml organic active microbial inoculums, it is organic cultivation, promotes the microorganism beneficial bacterium production technology of animal stalwartness rapid growth.The product of the present invention is with the natural Organic Ingredients of efficient organic principle, living bacteria count is made up to 2,800,000,000/ml agricultural microbial agent using the invention of domestic sophisticated technologies, it is that mother liquor chelates bacillus licheniformis, bacillus subtilis by amino acid, chelate two special bacillus, purpose is to be fed with efficient organic microbial bacterium by being added in feed, poultry, fish is improved the resistance to various germs.Have the function that with bacterium antibacterial, so as to ensure poultry, the survival rate of fish, reach the purpose of organic cultivation, ensure that the security of food, while also ensured the health of the mankind.
Description
Technical field
The present invention relates to technical field of microbial fermentation, is to be related to a kind of new microbe agent and its system specifically
Preparation Method.
Background technology
With the continuous development of reform and opening-up, agricultural modernization enters organic plantation and organic cultivation epoch.China is 70
Initial stage in age, the development of agricultural welcome chemical chemical fertilizer of new generation, i.e. nitrogen, phosphorus, potassium, fertilizer, and popularization throughout the country should
With making the tremendous lifting of agri-foodstuffs yield.Also it is same to animal husbandry, culture fishery on the premise of grain yield improves constantly
When flourish.Because chemical fertilizer is inorganic fertilizer, high and stable yields period is arrived although agri-foodstuffs can be lifted,
Generally using excessively, causing, quality of agricultural product declines inorganic fertilizer, its hazardous compound remains in grain in terms of China's agricultural
Among food, while make the long-term consumption such as the mankind, herding class and aquatic product containing the heavier grain of heavy metal element（Including its cultivation
Industry）Feed, the retained sediments of compound directly endanger the mankind and other animals among the liver of human body and animal, courage, kidney
The health of class and existence.Because animal husbandry for many years, culture fishery meet the exhibition of breaking out, feedstuff industry also arises at the historic moment, feed
Market competition, the nutrition-allocated proportion in proportioning is uneven, and the serious of the air pollution and water quality heavy metal plus current China surpasses
Mark, causes animal husbandry, aquatic products industry to bring serious bird flu and other diseases, the serious dead of poultry and aquatic fish shrimps occurs
Die, survival rate declines.
According to experience is cultivated for many years, the research staff of patent of the present invention finds out the master that influenza and other diseases occurs in animal
It is that air pollution and water quality heavy metals exceeding standard and forage compounding are uneven to want reason, causes a large amount of chemical residues of precipitation in animal body
Material, it is accumulated in poultry, the liver of fish, courage, kidney, enteron aisle and respiratory system, directly endangers animal health and existence.This hair
The technical staff of bright patent, by studying intensively for many years, for extreme illness normally, developing a new generation finally can Anti-bacterium
Nontoxic, novel pollution-free microbial bacterial agent.
The content of the invention
To achieve these goals, the invention provides a kind of new microbe agent and preparation method thereof, cultivation is passed through
The various nutrients of rational proportion in industry, are balanced proportioning in terms of cultivation, reach nutrient balance, strengthen immunity, neutralizing
Harmful substance reaches the robust growth for the purpose of bacterium antibacterial, being more beneficial for animal in animal body, solves professional raising farmer and wide
The cultivation difficulty problem of big nationality's tradition cultivation.
In order to solve the above-mentioned technical problem, the present invention is addressed by following technical proposals：
A kind of preparation method of new microbe agent, comprises the following steps：
（1）Fresh silkworm chrysalis is rinsed well with clear water and slicer is sent into after drying, broken specification 1-1.2CM, crush complete it is laggard
Enter 45 DEG C of thermostatic tanks, carry out constant temperature processing, pending good dry silkworm chrysalis absorption moisture to saturation state, be subsequently placed into fermentation tank,
With sodium hydroxide solution by volume 30:1：6 ratio carries out fermentation hydrolysis（30 water for referring to be carbonized through activated carbon are 30,1
Refer to the ratio progress that sodium hydroxide solution and 6 silkworm chrysalises handled well are converted into carbonization water and sodium hydroxide generation combination reaction
Fermentation hydrolysis）Ratio carry out fermentation hydrolysis, after 2-4 hour（Depending on weather temperature, more than 4 hours winter, high temperature day
Gas 2 hours or so）, fermented after pupa capsule absorbs moisture, after the completion of fermentation by centrifuge complete pupa capsule and pupa ginning outturn from,
Exclude poisonous pupa clothing slag, by the pupa capsule solution of separation enter fermentation plant carry out 36-48 hours be sealed by fermentation output pupa albumen
Alkaline mother liquor；
（2）By volume 20 after hair is cleaned with clear water:1：4（20, which refer to that the water through activated carbon carbonization refers to for 20,1, is being carbonized
Water produces combination reaction with sodium hydroxide and is converted into sodium hydroxide solution and the hair handled well, and 4 be hair）Ratio hydrogen-oxygen
Change sodium solution and enter oxygenation ferment in fermentation tank, carry out dehydration separation by centrifuge after the completion of fermentation, extract alkaline angle egg
In vain, it is left part slag and makees secondary fermentation reprocessing；The basic keratins and step extracted through partition method（1）Middle pupa egg
After white alkaline mother liquor mixing (alkaline hair-keratin mother liquor 30%, pupa capsule basic protein mother liquor 40%), with acetic acid, phosphoric acid or nitric acid
PH is adjusted in preferred pH=9 of 8.5-9.5, generates standby mother liquor；
（3）Animal bone through handling well is put into acidproof container and is impregnated with bone with strong acid, treats that bone is complete after 150-160 hours
Entirely by after strong acid catalyst, make 1,2,3 levels respectively for the stainless (steel) wire of 325 mesh numbers with 3 groups of standard mesh numbers and carry out voluntarily drip point
From after 3 grades of separation, strong acid can not be directly separated with centrifuge, can only be separated using multilayer water clock method, flow back into Sheng acid
Vessel, recovery drip isolate strong acid make it is next catalysis the secondary use of flow, through drip separation except acid system operation drip-dry deacidification
Afterwards, acid compound is generated；Preferred pH=5 of the regulation pH value 4.5-5.5 of the acid compound caustic lye of soda after acid are removed to obtain
Acid bone keratin mother liquor, by step（2）In standby mother liquor, with the bone keratin acid mother liquid chelate（Use ratio chelates
Method, hair basic keratins mother liquor 30%, pupa capsule basic protein mother liquor 40%, the keratin of bone are acidic protein mother liquor 30%, two
Basic protein is mixed into 70% basic protein mother liquor, is carried out by 70% basic protein mother liquor and 30% acid keratin mother liquor
Chelating method is mixed slowly to be chelated）Afterwards, adjusted with phosphoric acid, nitric acid or acetic acid between neutralizing pH value 6-8, so as to which output contains Guang
The aminoacids solution of the several amino acids such as propylhomoserin, methionine, lysine；
（4）By step（3）Middle aminoacids solution and other effective nutrients（Include potassium dihydrogen phosphate, zinc sulfate, magnesium sulfate, boron
Sand, sodium molybdate（Ammonium）, manganese sulfate, calcium nitrate, the micronutrient element such as potassium nitrate）With carbonization water hydrolysis, dissolved through carbonization water
Trace element produces combination reaction, and with the reaction of compound, its temperature carries out defibrination chelating when cooling to 17-18 DEG C automatically；
（5）By step（4）The product of middle chelating makees culture medium and carries out second to ferment, and the condition of fermentation will be controlled in 45 DEG C of constant temperature
And the environment of sealing；
（6）Step（5）Middle product adds glucose, brown sugar, honey, vitamin, monosodium glutamate after examining fermentation qualified, through 45 DEG C of perseverances
The fermentation of temperature chelating is cooled under normal temperature after 3 hours and is inoculated with 50% bacillus licheniformis, 50% bacillus subtilis（50% gemma bacterium number
It is effective bacterium number according to 1 ton of 2,800,000,000/ml of microbial bacterial agent of making, it is necessary to bacillus licheniformis and bacillus subtilis each one
Half, example：Need totally 14 kilograms of the special strain of above two, 7 kilograms of bacillus licheniformis, 7 kilograms of bacillus subtilis.Lichens bud
Spore bacillus, bacillus subtilis, two kinds of special strains are manufactured by Guangzhou industrial microorganism inspection center, including bacterium numbering is deposited
Hide, our company's directly purchase need not set strain and deposit Tibetan room, that is, carries and using）Third time fermentation lifting is chelated to ideal by oxygenation again
Numerical value, living bacteria count is produced up to 2,800,000,000/ml microbial bacterial agent；
（7）Examined using colony counting method, suitable culture medium is selected according to the species of institute's micrometer biology；
（8）Finished product, packaging.
Further, step（4）In, other described effective nutrients chelate according to each ton of hydrating solution by defibrination
6 kilograms of potassium dihydrogen phosphate of method addition, 12 kilograms of magnesium sulfate, 10 kilograms of borax, 5 kilograms of import copper sulphate, sodium molybdate（Ammonium）0.08
Kilogram, 0.2 kilogram of manganese sulfate, 1 kilogram of calcium nitrate, 10 kilograms of zinc sulfate, 1 kilogram of potassium nitrate, 0.7 kilogram of 32% phosphoric acid, nitric acid
0.7 kilogram carries out mixing slowly addition.
Further, 3 kilograms of glucose, 6 kilograms of brown sugar, 2 kilograms of honey, vitamin are added according to each ton of hydrating solution
20 grams, 15 grams of monosodium glutamate is added with the mode of mixing slowly（Pay attention to being unable to high-speed stirred, high-speed stirred can produce shearing force, kill
Hinder thalline, influence effective bacterium number）.
The invention further relates to a kind of new microbe agent, the new microbe agent is carried out according to described preparation method
Prepare.
The invention further relates to a kind of application method of new microbe agent, and the microbial bacterial agent of preparation is pressed into 1:3 times with
Pure water dilutes, then is used with feed mixed feeding.
A kind of new microbe agent of the present invention makees mother liquor using natural organic protein, chelating bacillus licheniformis, withered
Careless bacillus and various trace elements, through kinds of processes flow, deep processing refines out a new generation and contains 2,800,000,000/ml organic actives
Microbial inoculum, it is organic cultivation, promotes the microorganism beneficial bacterium production technology of animal stalwartness rapid growth.This microbial bacterial agent is with life
A kind of animal husbandry vast at this stage specifically for China, culture fishery development need are produced, use is pollution-free, nuisanceless, nothing
Residual, the organic cultivation that effect is good, more environmentally friendly.In order to adapt to China's animal husbandry, the change of culture fishery production structure, for
Herding, the disease of aquatic products industry, feed match micro- imbalance cause animal body inner structure to change and develop it is a kind of with
Organic protein is primary raw material, adds bacillus licheniformis, bacillus subtilis that organic active beneficial bacterium is made, balances in animal body
Growth needs, the microbial bacterial agent are derivative based on lactobacillus-fermented technical research.
The product of the present invention is made effectively with the natural Organic Ingredients of efficient organic principle using the invention of domestic sophisticated technologies
Microbial bacterial agent of the viable count up to 2,800,000,000/ml, it is therefore an objective to fed, made by being added in feed with efficient organic microbial bacterium
Poultry, fish improve the resistance to various germs.Have the function that with bacterium antibacterial, so as to ensure that poultry, fish survive
Rate, reach the purpose of organic cultivation, ensure that the security of food, while also ensure the health of the mankind.
Brief description of the drawings
Accompanying drawing described herein is used for providing a further understanding of the present invention, forms the part of the application, this hair
Bright schematic description and description is used to explain the present invention, does not form inappropriate limitation of the present invention.In the accompanying drawings：
Fig. 1 is the process chart of the preparation method of the new microbe agent of the present invention.
Embodiment
To make the purpose, technical scheme and advantage of the embodiment of the present invention clearer, below in conjunction with the embodiment of the present invention
Accompanying drawing, the technical scheme of the embodiment of the present invention is clearly and completely described.Obviously, described embodiment is this hair
Bright part of the embodiment, rather than whole embodiments.Based on described embodiments of the invention, ordinary skill
The every other embodiment that personnel are obtained on the premise of without creative work, belongs to the scope of protection of the invention.
Specific embodiment：
A kind of preparation method of new microbe agent of the present invention comprises the following steps：
1. fresh silkworm chrysalis is rinsed well with clear water and slicer is sent into after drying, broken specification 1-1.2CM, enter after the completion of broken
45 DEG C of thermostatic tanks, carry out constant temperature processing, and pending good dry silkworm chrysalis absorbs moisture to saturation state, is subsequently placed into fermentation tank, uses
Sodium hydroxide solution by volume 30:1：6 ratio carries out fermentation hydrolysis（30 water for referring to be carbonized through activated carbon refer to for 30,1
The ratio that sodium hydroxide solution and 6 silkworm chrysalises handled well are converted into carbonization water and sodium hydroxide generation combination reaction is sent out
Ferment hydrolyzes）Ratio carry out fermentation hydrolysis, after 2-4 hour（Depending on weather temperature, more than 4 hours winter, hot weather 2
Hour or so）, fermented after pupa capsule absorbs moisture, pupa capsule completed with pupa ginning outturn from row by centrifuge after the completion of fermentation
Except poisonous pupa clothing slag, the pupa capsule solution of separation is entered into fermentation plant carry out 36-48 hours and be sealed by fermentation output pupa albumen alkali
Aqueous mother liquor.
There is ancient Chinese prose to record 6 silkworm chrysalises and be equal to 1 egg, silkworm chrysalis contains high natural nutrition value, and pupa capsule is containing abundant
Albumen is accounted between 51-56%.The research staff of the present invention contains one or more of toxic substances through pupa clothing of exploring for many years, this
Kind is its self-defense function containing poisoning material, there is adverse reaction of itching after someone is edible, and containing for silkworm chrysalis is learnt in exploration
Noxious material is in pupa clothing epidermis, so pupa clothing must be completely separated totally with pupa capsule.
2. by volume 20 after hair is cleaned with clear water:1：4（20 refer to that the water through activated carbon carbonization refers in carbon for 20,1
Change water and be converted into sodium hydroxide solution and the hair handled well with sodium hydroxide generation combination reaction, 4 be hair）Ratio hydrogen
Sodium hydroxide solution enters oxygenation ferment in fermentation tank, carries out dehydration separation by centrifuge after the completion of fermentation, extracts alkaline angle
Albumen, remaining part slag make secondary fermentation reprocessing；The basic keratins extracted through partition method and pupa egg in step 1
After white alkaline mother liquor mixing (alkaline hair-keratin mother liquor 30%, pupa capsule basic protein mother liquor 40%), with acetic acid, phosphoric acid or nitric acid
PH is adjusted in preferred pH=9 of 8.5-9.5, generates standby mother liquor.
3. the animal bone through handling well is put into acidproof container is impregnated with bone with strong acid, bone is treated after 150-160 hours
Head by after strong acid catalyst, is made 1,2,3 levels for the stainless (steel) wire of 325 mesh numbers with 3 groups of standard mesh numbers and voluntarily dripped respectively completely
Flow separation, after 3 grades of separation, strong acid can not be directly separated with centrifuge, can only be separated, flowed back into using multilayer water clock method
The vessel of acid are contained, recovery drip isolates strong acid and makees the next catalysis secondary use of flow, through drip separation except acid system operation drip-dry
After deacidification, acid compound is generated；Remove the acid compound after acid and adjust preferred pH=5 of pH value 4.5-5.5 with caustic lye of soda
Acid bone keratin mother liquor is obtained, standby mother liquor in step 2 chelates with the bone keratin acid mother liquid（Use ratio chela
Legal, hair basic keratins mother liquor 30%, pupa capsule basic protein mother liquor 40%, the keratin of bone is acidic protein mother liquor 30%, two
Individual basic protein is mixed into 70% basic protein mother liquor, is entered by 70% basic protein mother liquor and 30% acid keratin mother liquor
Row mixes slowly chelating method and chelated）Afterwards, adjusted with phosphoric acid, nitric acid or acetic acid between neutralizing pH value 6-8, so as to which output contains
The aminoacids solution of the several amino acids such as cystine, methionine, lysine.
4. by aminoacids solution in step 3 and other effective nutrients（Comprising potassium dihydrogen phosphate, zinc sulfate, magnesium sulfate,
Borax, sodium molybdate（Ammonium）, manganese sulfate, calcium nitrate, the micronutrient element such as potassium nitrate）With carbonization water hydrolysis, through being carbonized, water dissolves
Trace element produce combination reaction, with the reaction of compound, defibrination chela is carried out when its temperature cools to 17-18 DEG C automatically
Close.
, each ton of hydrating solution is by 6 kilograms of potassium dihydrogen phosphate of defibrination chelating method addition, 12 kilograms of magnesium sulfate, borax 10
Kilogram, 5 kilograms of import copper sulphate, sodium molybdate（Ammonium）0.08 kilogram, 0.2 kilogram of manganese sulfate, 1 kilogram of calcium nitrate, zinc sulfate 10,000
Gram, 1 kilogram of potassium nitrate, 0.7 kilogram of 32% phosphoric acid, 0.7 kilogram of nitric acid carries out mixing slowly addition.
Fermented 5. the product chelated in step 4 is made into culture medium and carries out second, the condition of fermentation will be controlled in constant temperature 45
DEG C and sealing environment.
6. in step 5 product after examining fermentation qualified 3 kilograms of glucose of each ton of hydrating solution addition, 6 kilograms of brown sugar,
2 kilograms of honey, 20 grams of vitamin, 15 grams of monosodium glutamate are added with the mode of mixing slowly（Pay attention to being unable to high-speed stirred, high-speed stirred
Shearing force can be produced, thalline is killed, influences effective bacterium number）, it is cooled under normal temperature and is inoculated with after 45 DEG C of constant temperature chelatings ferment 3 hours
50% bacillus licheniformis, 50% bacillus subtilis（50% gemma bacterium number is having according to 1 ton of 2,800,000,000/ml of microbial bacterial agent of making
Bacterium number is imitated, it is necessary to bacillus licheniformis and each half of bacillus subtilis, example：Totally 14 kilograms of the special strain of above two is needed,
7 kilograms of bacillus licheniformis, 7 kilograms of bacillus subtilis.Bacillus licheniformis, bacillus subtilis, two kinds of special strains are equal
By Guangzhou, industrial microorganism inspection center manufactures, including bacterium numbering deposits Tibetan, and our company's directly purchase need not set strain and deposit Tibetan room,
Carry and using）Third time fermentation lifting is chelated by oxygenation again and arrives desired quantity, produces living bacteria count up to the micro- of 2,800,000,000/ml
Bacteria agent.
7. being examined using colony counting method, suitable culture medium is selected according to the species of institute's micrometer biology.
8. finished product, packaging.
Bacillus licheniformis, the effect of bacillus subtilis are as follows in the present invention：
Bacillus licheniformis：
1. resisting animal pathogen and maintaining and adjust stomach microecological balance, i.e. negative and positive reconcile, and it is harmful to suppress Escherichia coli etc.
The growth and breeding of bacterium, promote the growth and breeding of the beneficial bacteriums such as lactic acid bacteria.Play a part of reducing or substituting antibiotic.
2. producing a variety of enzymes and improving animal digestion enzymatic activity, and the activity of animal digestion enzyme is greatly enhanced, promote to support
The growth of animal is grown, improves feed utilization rate.
3. strengthening poultry, the immunologic function of fish, body specificity and nonspecific immune reaction can be strengthened, promote macrophage
The phagocytic activity of cell, improve anti-infection ability.
4. improving environment, the discharge of animal wastes moderate odor can be significantly reduced by taking bacillus licheniformis preparation, improved and supported
Grow environment.
5. aquatic livestock, stimulating the development of aquatic livestock immune organ, strengthen immunity of organisms, effectively prevent aquatic livestock
The diseases such as enterogastritis, gill rot；With stronger protease, lipase, amylase activity, promote feed in nutrient drop
Solution, makes aquatic product animal more abundant to absorbing for feed.
Bacillus subtilis：
1. can resistance to oxidation, high temperature resistant, can be survived in 50 ~ 60 DEG C of high temperature, resistance to acids and bases, being can be 100% straight in current feed microbe
Up to the viable bacteria of intestine and small intestine.
2. the stomach tract disease such as pre- anti-diarrhea, diarrhea.
3. promotion is digested and assimilated, efficiency of feed utilization is improved, prevents that animal digestion is bad.
4. the use of antibiotic medicine, strengthen immunity can be reduced.
5. controlling bacteriosis, fly is driven in deodorization, reduces environmental pollution.
6. improving meat, eggs and milk quality, it can naturally increase animal product colourity and edible flavor, pig skin is ruddy, hair color hair
Bright, broiler chicken, meat duck color burn, fast fertile, fast length, aquatic livestock color is good, whets the appetite, more robust growth.
During use, this microbial bacterial agent belongs to highly enriched fermented liquid, it is impossible to direct feeding；Need to use microbial bacterial agent
By 1:3 times dilute with pure water, then are used with feed mixed feeding；It can not be used in mixed way with other animal insecticides.
The invention of this microbial bacterial agent, be advantageous to the vast agricultural in China, animal husbandry, the development need of culture fishery, create
Organic plantation, organic cultivation new science and technology have been made, a new organic resource is started for country and nationality, will with the technology of the present invention
Hair, the silkworm chrysalis of silk mill that countless remaining meals remaining bone after meal and hairdressing place cut be discarded object, will now be worked out for many years
Decompose toxicity removing method exclude its toxic material, turn waste into wealth, create effective bacterium number up to 2,800,000,000/ml microbial bacterial agent for country
Reduce environmental pollution, be that to create organic resource of new generation be that agricultural, animal husbandry, culture fishery are contributed for country again.
Finally illustrate, the above embodiments are merely illustrative of the technical solutions of the present invention and it is unrestricted, although with reference to compared with
The present invention is described in detail good embodiment, it will be understood by those within the art that, can be to the skill of the present invention
Art scheme is modified or equivalent substitution, and without departing from the objective and scope of technical solution of the present invention, it all should cover at this
Among the right of invention.
Claims (5)
1. a kind of preparation method of new microbe agent, it is characterised in that comprise the following steps：
（1）Fresh silkworm chrysalis is rinsed well with clear water and slicer is sent into after drying, broken specification 1-1.2CM, crush complete it is laggard
Enter 45 DEG C of thermostatic tanks, carry out constant temperature processing, pending good dry silkworm chrysalis absorption moisture to saturation state, be subsequently placed into fermentation tank,
With sodium hydroxide solution by volume 30:1:6 ratio carries out fermentation hydrolysis（30 water for referring to be carbonized through activated carbon are 30,1
Refer to the ratio progress that sodium hydroxide solution and 6 silkworm chrysalises handled well are converted into carbonization water and sodium hydroxide generation combination reaction
Fermentation hydrolysis）, after 2-4 hour（Depending on weather temperature, more than 4 hours winter, hot weather 2 hours or so）, treat pupa
Capsule is fermented after absorbing moisture, after the completion of fermentation by centrifuge complete pupa capsule and pupa ginning outturn from, exclude poisonous pupa clothing slag,
By the pupa capsule solution of separation enter fermentation plant carry out 36-48 hours be sealed by fermentation output pupa albumen alkaline mother liquor；
（2）By volume 20 after hair is cleaned with clear water:1:4（20, which refer to that the water through activated carbon carbonization refers to for 20,1, is being carbonized
Water produces combination reaction with sodium hydroxide and is converted into sodium hydroxide solution and the hair handled well, and 4 refer to hair）Ratio hydrogen
Sodium oxide molybdena hydrating solution enters oxygenation ferment in fermentation tank, carries out dehydration separation by centrifuge after the completion of fermentation, extracts alkali
Property keratin, remaining part slag makees secondary fermentation reprocessing；The basic keratins and step extracted through partition method（1）
After middle pupa albumen alkaline mother liquor mixing (alkaline hair-keratin mother liquor 30%, pupa capsule basic protein mother liquor 40%), with acetic acid, phosphoric acid
Or nitre acid for adjusting pH generates standby mother liquor in preferred pH=9 of 8.5-9.5；
（3）Animal bone through handling well is put into acidproof container and is impregnated with bone with strong acid, treats that bone is complete after 150-160 hours
Entirely by after strong acid catalyst, make 1,2,3 levels respectively for the stainless (steel) wire of 325 mesh numbers with 3 groups of standard mesh numbers and carry out voluntarily drip point
From after 3 grades of separation, strong acid can not be directly separated with centrifuge, can only be separated using multilayer water clock method, flow back into Sheng acid
Vessel, recovery drip isolate strong acid make it is next catalysis the secondary use of flow, through drip separation except acid system operation drip-dry deacidification
Afterwards, acid compound is generated；Preferred pH=5 of the regulation pH value 4.5-5.5 of the acid compound caustic lye of soda after acid are removed to obtain
Acid bone keratin mother liquor, by step（2）In standby mother liquor, with the bone keratin acid mother liquid chelate（Use ratio chelates
Method, hair basic keratins mother liquor 30%, pupa capsule basic protein mother liquor 40%, the keratin of bone are acidic protein mother liquor 30%, two
Basic protein is first mixed into 70% basic protein mother liquor, then by 70% basic protein mother liquor and 30% acid keratin mother liquor
Carry out mixing slowly chelating method and chelated）Afterwards, adjusted with acetic acid, nitric acid or phosphoric acid between neutralizing pH value 6-8, so as to which output contains
There is the aminoacids solution of the several amino acids such as cystine, methionine, lysine；
（4）By step（3）Middle aminoacids solution and other effective nutrients（Include potassium dihydrogen phosphate, zinc sulfate, magnesium sulfate, boron
Sand, sodium molybdate（Ammonium）, manganese sulfate, calcium nitrate, the micronutrient element such as potassium nitrate）With carbonization water hydrolysis, dissolved through carbonization water
Trace element produces combination reaction, and with the reaction of compound, its temperature carries out defibrination chelating when cooling to 17-18 DEG C automatically
（Defibrination chelates：Carry out combination reaction is mixed by grinding machine）.
（5）By step（4）The product of middle chelating makees culture medium and carries out second to ferment, and the condition of fermentation will be controlled in 45 DEG C of constant temperature
And the environment of sealing；
（6）Step（5）Middle product adds glucose, brown sugar, honey, vitamin, monosodium glutamate after examining fermentation qualified, through 45 DEG C of perseverances
The fermentation of temperature chelating is cooled under normal temperature after 3 hours and is inoculated with 50% bacillus licheniformis, 50% bacillus subtilis（50% gemma bacterium number
It is effective bacterium number according to 1 ton of 2,800,000,000/ml of microbial bacterial agent of making, it is necessary to bacillus licheniformis and bacillus subtilis each one
Half, example：Need totally 14 kilograms of the special strain of above two, 7 kilograms of bacillus licheniformis, 7 kilograms of bacillus subtilis.
Bacillus licheniformis, bacillus subtilis, two kinds of special strains are manufactured by Guangzhou industrial microorganism inspection center, including
Bacterium numbering deposits Tibetan, and our company's directly purchase need not set strain and deposit Tibetan room, that is, carry and using）Third time is chelated by oxygenation again to ferment
Desired quantity is lifted, produces living bacteria count up to 2,800,000,000/ml microbial bacterial agent；
（7）Examined using colony counting method, suitable culture medium is selected according to the species of institute's micrometer biology；
（8）Finished product, packaging.
2. the preparation method of new microbe agent according to claim 1, it is characterised in that：Step（4）In, it is described its
His effective nutrient chelates method by defibrination according to each ton of hydrating solution and adds 6 kilograms of potassium dihydrogen phosphate, magnesium sulfate 12,000
Gram, 10 kilograms of borax, 5 kilograms of import copper sulphate, sodium molybdate（Ammonium）0.08 kilogram, 0.2 kilogram of manganese sulfate, 1 kilogram of calcium nitrate, sulphur
Sour 10 kilograms of zinc, 1 kilogram of potassium nitrate, 0.7 kilogram of 32% phosphoric acid, 0.7 kilogram of nitric acid carry out mixing slowly addition.
3. the preparation method of new microbe agent according to claim 1 or 2, it is characterised in that：According to each ton of water
3 kilograms of glucose of solution solution addition, 6 kilograms of brown sugar, 2 kilograms of honey, 20 grams of vitamin, 15 grams of monosodium glutamate are entered with the mode of mixing slowly
Row addition（Pay attention to being unable to high-speed stirred, high-speed stirred can produce shearing force, kill thalline, influence effective bacterium number）.
4. a kind of new microbe agent, it is characterised in that the new microbe agent is any described according to claim 1-3
It is prepared by preparation method.
5. the application method of the new microbe agent described in claim 4, it is characterised in that：The microbial bacterial agent of preparation is pressed
1:3 times dilute with pure water, then are used with feed mixed feeding.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103044130A (en) * | 2012-08-13 | 2013-04-17 | 佛山市南海区腾龙微生物肥料厂 | Agriculture microbial agent formulation and preparation method thereof |
| CN105053677A (en) * | 2015-08-13 | 2015-11-18 | 黄海娟 | Freshwater fish feed |
| CN105410482A (en) * | 2015-12-23 | 2016-03-23 | 德阳市嘉隆农业有限公司 | Nutritional fish feed |
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