CN107502451A - The preparation method of Rosmarinus officinalis extract - Google Patents
The preparation method of Rosmarinus officinalis extract Download PDFInfo
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- CN107502451A CN107502451A CN201710823888.2A CN201710823888A CN107502451A CN 107502451 A CN107502451 A CN 107502451A CN 201710823888 A CN201710823888 A CN 201710823888A CN 107502451 A CN107502451 A CN 107502451A
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
- C11B9/027—Recovery of volatiles by distillation or stripping
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D11/00—Solvent extraction
- B01D11/02—Solvent extraction of solids
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C51/00—Preparation of carboxylic acids or their salts, halides or anhydrides
- C07C51/42—Separation; Purification; Stabilisation; Use of additives
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C51/00—Preparation of carboxylic acids or their salts, halides or anhydrides
- C07C51/42—Separation; Purification; Stabilisation; Use of additives
- C07C51/47—Separation; Purification; Stabilisation; Use of additives by solid-liquid treatment; by chemisorption
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C67/00—Preparation of carboxylic acid esters
- C07C67/48—Separation; Purification; Stabilisation; Use of additives
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C67/00—Preparation of carboxylic acid esters
- C07C67/48—Separation; Purification; Stabilisation; Use of additives
- C07C67/56—Separation; Purification; Stabilisation; Use of additives by solid-liquid treatment; by chemisorption
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- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D493/00—Heterocyclic compounds containing oxygen atoms as the only ring hetero atoms in the condensed system
- C07D493/02—Heterocyclic compounds containing oxygen atoms as the only ring hetero atoms in the condensed system in which the condensed system contains two hetero rings
- C07D493/08—Bridged systems
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
- C11B9/022—Refining
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Abstract
The present invention relates to plant extracts, more particularly to a kind of preparation method of Rosmarinus officinalis extract, harvesting rosemary leaf is crushed to 30 80 mesh；The rosemary crushed is added in round-bottomed flask, distillation extraction Rosemary Oil is carried out, rosemary leaf slag is poured into blowing-type drying machine, temperature is adjusted to 50 70 DEG C, 7 9h is dried, by leaf slag according to solid-liquid ratio 1 after drying:8 add purity as 90% 99% edible alcohols, and on 45 65 DEG C of water-baths, the 2.5h of heating extraction 1.5 collects extract solution, and extraction twice and merges, just comprising rosemary water-soluble extractive and fat-soluble extract in the extract solution；By alcohol recycle in extract solution, then extract solution concentration carries out filtered on buchner funnel, and the filter residue after filtering, which is poured into dryer, dries, and produces rosemary fat-soluble extract, and the filtrate concentration, spray drying after filtering had both obtained the water-soluble extraction of rosemary；The present invention improves operating efficiency, cost-effective.
Description
Technical field
The present invention relates to the preparation method of plant extracts, more particularly to a kind of preparation method of Rosmarinus officinalis extract.
Background technology
Rosemary system Labiatae Rosmarinus plant, it is widely used, except cook when as spices in addition to, pharmacologically also kill
Bacterium, the anti-oxidant, effect of refreshing the mind etc., the essential oil of refinement can manufacture cologne, add shampoo and also can remove dandruff, by stem
Leaf is put into take a shower in water for bathing and can stimulated circulation etc..In addition, rosemary is that generally acknowledged at present have high anti-oxidation
A kind of plant of effect.At present, it is domestic for rosemary be processed and extract the production technology of effective extract still not into
Ripe, wherein some processing mode can only extract a kind of active ingredient, for example, disclosed Application No.
201410445684 .6 patent application《A kind of method that Rosmarinic acid is extracted from rosemary》, it is proposed that using biology enzyme
The technical scheme of fermentation assisted extraction Rosmarinic acid, it is only capable of extracting a kind of composition of Rosmarinic acid from rosemary, it is impossible to
Other active ingredients are effectively extracted simultaneously, cause the loss and waste of other active ingredients in rosemary.Common rosemary
Extraction process is using high temperature extraction industrial procedure, and both energy loss, also destroyed ultimate constituent.
The content of the invention
It is an object of the invention to overcome the deficiencies in the prior art, avoids the step when extracting rosemary excessively complicated, wave
Take resource, and provide a kind of preparation method of Rosmarinus officinalis extract, the present invention is using step normal temperature method extraction separation rosemary
Rosemary is fat-soluble and water-soluble extractive, improves efficiency and extract quality, cost-effective.
The purpose of the present invention is achieved by the following measures：A kind of preparation method of Rosmarinus officinalis extract, including such as
Lower step：
（1）Harvesting：The fresh rosemary leaf of harvesting is removed into stem, dries, is crushed to 30-80 mesh, lucifuge Cord blood is standby；
（2）Extraction by steam distillation Rosemary Oil：By step（1）The rosemary crushed is added in round-bottomed flask, by material
Liquor ratio 0.8-1.2:10 add pure water, and the rosemary crushed is material, and pure water is liquid, and distillation extraction 1-2h, distillation obtains
Liquid carry out water-oil separating with separatory funnel, collect the coarse-fine oil of rosemary, adding anhydrous sodium sulfate to the coarse-fine oil of rosemary enters
Row dehydration, filters to obtain finished product Rosemary Oil；
（3）Normal temperature method extracts Rosmarinus officinalis extract：Step（2）Rosemary leaf slag pour into blowing-type drying machine, temperature is adjusted to
50-70 DEG C, 7-9h is dried, leaf slag is put into round-bottomed flask after drying, according to solid-liquid ratio 0.7-1.3:8 addition purity are 90%-
99% edible alcohol, melt cinder are material, and alcohol is liquid, is placed on 45-65 DEG C of water-bath, heating extraction 1.5-2.5h, collect extraction
Liquid, similarity condition extract once again, merge extract solution twice, just comprising rosemary water-soluble extractive and liposoluble in the extract solution
Property extract；
（4）Concentration method separates Rosmarinus officinalis extract：By step（3）Middle extract solution is concentrated with vacuum and low temperature rotary evaporation instrument
Recovered alcohol, when being concentrated into the 8%-13% that volume is extracting liquid volume, the extract solution after concentration is passed through Buchner funnel, adds water to enter
Row filtering, the filter residue after filtering is poured into dryer, and temperature is adjusted to 50-65 DEG C, dries 7-9h, as rosemary is fat-soluble carries
Thing is taken, when to be concentrated into proportion be 1-1.5 by the filtrate after filtering, proportion herein is the numerical value that densimeter is shown, is sprayed
Dry and both obtain the water-soluble extraction of rosemary, detected by HLCP methods, fat-soluble extract carnosic acid and carnosol content are total
20%-25% is calculated as, water-soluble extractive rosmarinic acid contents are 7%-9%.
Preferably, the preparation method of described Rosmarinus officinalis extract, comprises the following steps：
（1）Harvesting：The fresh rosemary leaf of harvesting is removed into stem, dries, is crushed to 50 mesh, lucifuge Cord blood is standby；
（2）Extraction by steam distillation Rosemary Oil：By step（1）The rosemary crushed is added in round-bottomed flask, by material
Liquor ratio 1:10 add pure water, distillation extraction 1.5h, and the liquid for distilling to obtain carries out water-oil separating with separatory funnel, collects fan and change
Fragrant coarse-fine oil, add anhydrous sodium sulfate to the coarse-fine oil of rosemary and be dehydrated, filter to obtain finished product Rosemary Oil；Add in this step
It is in order to which moisture is separated to enter anhydrous sodium sulfate；
（3）Normal temperature method extracts Rosmarinus officinalis extract：Step（2）Rosemary leaf slag pour into blowing-type drying machine, temperature is adjusted to
60 DEG C, 8h is dried, leaf slag is put into round-bottomed flask after drying, according to solid-liquid ratio 1:8 addition purity are 95% edible alcohol, are put
Put on 55 DEG C of water-baths, heating extraction 2h, collect extract solution, similarity condition extracts once again, merges extract solution twice, this is carried
Take in liquid just comprising rosemary water-soluble extractive and fat-soluble extract；This step add alcohol be because alcohol it is volatile,
And water is dissolved in, it is easier so being placed on water-bath to carry out extracting rosemary water-soluble extractive and fat-soluble extract；
（4）Concentration method separates Rosmarinus officinalis extract：By step（3）Middle extract solution is concentrated with vacuum and low temperature rotary evaporation instrument
Recovered alcohol, be concentrated into volume be extracting liquid volume 10% when, the extract solution after concentration is passed through Buchner funnel, adds water to carry out
Filtering, the filter residue after filtering is poured into dryer, and temperature is adjusted to 60 DEG C, dries 8h, as rosemary fat-soluble extract, will
Filtrate after filtering be concentrated into proportion for 1.2 when, be spray-dried both the water-soluble extraction of rosemary, examined by HLCP methods
Survey, fat-soluble extract carnosic acid and carnosol content add up to 23.1%, and water-soluble extractive rosmarinic acid contents are
8.6%。
Normal temperature in present invention process applies to the temperature on water-bath in the technique, and normal temperature is usually 45-65 DEG C, high
Temperature is 80-95 DEG C.
The beneficial effects of the invention are as follows：Height edible alcohol normal temperature extracts, and can both ensure the complete extraction of target component,
Low temperature can both reduce energy loss simultaneously, and other materials that can prevent chlorophyll again are extracted, and reduce rosemary
Target component is in the destruction of high temperature extraction process, raising product quality in extract.The technique one-step method is extracted in rosemary
Essential oil, water-soluble extractive, fat-soluble extract, the active ingredient in rosemary being made full use of, technique is simple, simple to operate,
Economize on resources.
Embodiment
In order to deepen the understanding of the present invention, below in conjunction with embodiment, the invention will be further described.
Embodiment 1：A kind of preparation method of Rosmarinus officinalis extract, comprises the following steps：
（1）Harvesting：The fresh rosemary leaf of harvesting is removed into stem, dries, is crushed to 30 mesh, lucifuge Cord blood is standby；
（2）Extraction by steam distillation Rosemary Oil：By step（1）The rosemary crushed is added in round-bottomed flask, by material
Liquor ratio 1:10 add pure water, distillation extraction 1h, and the liquid for distilling to obtain carries out water-oil separating with separatory funnel, collects rosemary
Coarse-fine oil, add anhydrous sodium sulfate to the coarse-fine oil of rosemary and be dehydrated, filter to obtain finished product Rosemary Oil；
（3）Normal temperature method extracts Rosmarinus officinalis extract：Step（2）Rosemary leaf slag pour into blowing-type drying machine, temperature is adjusted to
50 DEG C, 7h is dried, leaf slag is put into round-bottomed flask after drying, according to solid-liquid ratio 1:8 addition purity are 90% edible alcohol, are put
Put on 45 DEG C of water-baths, heating extraction 1.5h, collect extract solution, similarity condition extracts once again, merges extract solution twice, should
Just comprising rosemary water-soluble extractive and fat-soluble extract in extract solution；
（4）Concentration method separates Rosmarinus officinalis extract：By step（3）Middle extract solution is concentrated with vacuum and low temperature rotary evaporation instrument
Recovered alcohol, be concentrated into volume be extracting liquid volume 10% when, the extract solution after concentration is passed through Buchner funnel, adds water to carry out
Filtering, the filter residue after filtering is poured into dryer, and temperature is adjusted to 50 DEG C, dries 7h, as rosemary fat-soluble extract, will
Filtrate after filtering be concentrated into proportion for 1.2 when, be spray-dried both the water-soluble extraction of rosemary, examined by HLCP methods
Survey, fat-soluble extract carnosic acid and carnosol content add up to 20%, and water-soluble extractive rosmarinic acid contents are 7%.
Embodiment 2：A kind of preparation method of Rosmarinus officinalis extract, comprises the following steps：
（1）Harvesting：The fresh rosemary leaf of harvesting is removed into stem, dries, is crushed to 80 mesh, lucifuge Cord blood is standby；
（2）Extraction by steam distillation Rosemary Oil：By step（1）The rosemary crushed is added in round-bottomed flask, by material
Liquor ratio 1:10 add pure water, distillation extraction 2h, and the liquid for distilling to obtain carries out water-oil separating with separatory funnel, collects rosemary
Coarse-fine oil, add anhydrous sodium sulfate to the coarse-fine oil of rosemary and be dehydrated, filter to obtain finished product Rosemary Oil；
（3）Normal temperature method extracts Rosmarinus officinalis extract：Step（2）Rosemary leaf slag pour into blowing-type drying machine, temperature is adjusted to
70 DEG C, 9h is dried, leaf slag is put into round-bottomed flask after drying, according to solid-liquid ratio 1:8 addition purity are 90%-99% edible wines
Essence, it is placed on 65 DEG C of water-baths, heating extraction 2.5h, collects extract solution, similarity condition is extracted once again, and merging is extracted twice
Liquid, just comprising rosemary water-soluble extractive and fat-soluble extract in the extract solution；
（4）Concentration method separates Rosmarinus officinalis extract：By step（3）Middle extract solution is concentrated with vacuum and low temperature rotary evaporation instrument
Recovered alcohol, be concentrated into volume be extracting liquid volume 10% when, the extract solution after concentration is passed through Buchner funnel, adds water to carry out
Filtering, the filter residue after filtering is poured into dryer, and temperature is adjusted to 65 DEG C, dries 9h, as rosemary fat-soluble extract, will
Filtrate after filtering be concentrated into proportion for 1.2 when, be spray-dried both the water-soluble extraction of rosemary, examined by HLCP methods
Survey, fat-soluble extract carnosic acid and carnosol content add up to 25%, and water-soluble extractive rosmarinic acid contents are 9%.
Embodiment 3：A kind of preparation method of Rosmarinus officinalis extract, comprises the following steps：
（1）Harvesting：The fresh rosemary leaf of harvesting is removed into stem, dries, is crushed to 50 mesh, lucifuge Cord blood is standby；
（2）Extraction by steam distillation Rosemary Oil：By step（1）The rosemary crushed is added in round-bottomed flask, by material
Liquor ratio 1:10 add pure water, distillation extraction 1.5h, and the liquid for distilling to obtain carries out water-oil separating with separatory funnel, collects fan and change
Fragrant coarse-fine oil, add anhydrous sodium sulfate to the coarse-fine oil of rosemary and be dehydrated, filter to obtain finished product Rosemary Oil；
（3）Normal temperature method extracts Rosmarinus officinalis extract：Step（2）Rosemary leaf slag pour into blowing-type drying machine, temperature is adjusted to
60 DEG C, 8h is dried, leaf slag is put into round-bottomed flask after drying, according to solid-liquid ratio 1:8 addition purity are 95% edible alcohol, are put
Put on 55 DEG C of water-baths, heating extraction 2h, collect extract solution, similarity condition extracts once again, merges extract solution twice, this is carried
Take in liquid just comprising rosemary water-soluble extractive and fat-soluble extract；
（4）Concentration method separates Rosmarinus officinalis extract：By step（3）Middle extract solution is concentrated with vacuum and low temperature rotary evaporation instrument
Recovered alcohol, be concentrated into volume be extracting liquid volume 10% when, the extract solution after concentration is passed through Buchner funnel, adds water to carry out
Filtering, the filter residue after filtering is poured into dryer, and temperature is adjusted to 60 DEG C, dries 8h, as rosemary fat-soluble extract, will
Filtrate after filtering be concentrated into proportion for 1.2 when, be spray-dried both the water-soluble extraction of rosemary, examined by HLCP methods
Survey, fat-soluble extract carnosic acid and carnosol content add up to 23.1%, and water-soluble extractive rosmarinic acid contents are
8.6%。
Embodiment 4：A kind of preparation method of Rosmarinus officinalis extract, comprises the following steps：
（1）Harvesting：The fresh rosemary leaf of harvesting is removed into stem, dries, is crushed to 45 mesh, lucifuge Cord blood is standby；
（2）Extraction by steam distillation Rosemary Oil：By step（1）The rosemary crushed is added in round-bottomed flask, by material
Liquor ratio 1:10 add pure water, distillation extraction 1-2h, and the liquid for distilling to obtain carries out water-oil separating with separatory funnel, collects fan and change
Fragrant coarse-fine oil, add anhydrous sodium sulfate to the coarse-fine oil of rosemary and be dehydrated, filter to obtain finished product Rosemary Oil；
（3）Normal temperature method extracts Rosmarinus officinalis extract：Step（2）Rosemary leaf slag pour into blowing-type drying machine, temperature is adjusted to
60 DEG C, 9h is dried, leaf slag is put into round-bottomed flask after drying, according to solid-liquid ratio 1:8 addition purity are 93% edible alcohol, are put
Put on 60 DEG C of water-baths, heating extraction 1.5h, collect extract solution, similarity condition extracts once again, merges extract solution twice, should
Just comprising rosemary water-soluble extractive and fat-soluble extract in extract solution；
（4）Concentration method separates Rosmarinus officinalis extract：By step（3）Middle extract solution is concentrated with vacuum and low temperature rotary evaporation instrument
Recovered alcohol, be concentrated into volume be extracting liquid volume 10% when, the extract solution after concentration is passed through Buchner funnel, adds water to carry out
Filtering, the filter residue after filtering is poured into dryer, and temperature is adjusted to 50 DEG C, dries 8h, as rosemary fat-soluble extract, will
Filtrate after filtering be concentrated into proportion for 1.2 when, be spray-dried both the water-soluble extraction of rosemary, examined by HLCP methods
Survey, fat-soluble extract carnosic acid and carnosol content add up to 24%, and water-soluble extractive rosmarinic acid contents are 8%.
Be respectively adopted 55 DEG C of normal temperature for the extraction Rosmarinus officinalis extract in the present invention, add purity be 95% alcohol and
80 DEG C of high temperature, add under the conditions of purity is 75% and extracted, test, it is as follows：
1st, rosemary Fresh leaves are gathered, are dried under natural conditions, cured leaf is crushed to 50 mesh, weighs 100g and is put into 1000mL beakers
In, according to solid-liquid ratio 1：8 add 95% edible alcohol 800mL, are placed on water-bath, and sealed beaker is open, 55 degree, extract 2h,
Similarity condition extracts 2 times, merges extract solution, cryogenic vacuum rotary evaporation twice and is concentrated into the 10% of extracting liquid volume, filters, receive
Collect filter residue, 60 degree, dry 8h, filtrate is concentrated into 20% volume, is spray-dried, and detection water-soluble extractive and fat-soluble carries respectively
Take the yield of thing, the content of target component.
2nd, rosemary Fresh leaves are gathered, are dried under natural conditions, cured leaf is crushed to 50 mesh, weighs 100g and is put into 1000mL
In soft bottom flask, according to solid-liquid ratio 1：8 are separately added into 75% edible alcohol 800mL, are refluxing extraction in 80 DEG C of heating mantles in temperature
Twice, each 2h, merge extract solution, cryogenic vacuum rotary evaporation twice and be concentrated into the 10% of extracting liquid volume, filter, collect filter
Slag, 60 degree, 8h is dried, filtrate is concentrated into 20% volume, is spray-dried, and detects water-soluble extractive and fat-soluble extract respectively
Yield, the content of target component.
Following table is the upper yield and content for testing Rosmarinus officinalis extract under 1,2 different conditions：
As can be known from the above table, the yield of normal temperature extraction method fat-soluble extract improves 49.8%, and carnosic acid content somewhat lowers,
But carnosol content significantly reduces；Water-soluble extractive yield improves 128.2%, and rosmarinic acid contents are brought up to by 4.8%
6.7%.Synthesis show that a step normal temperature extracts rosemary method, can significantly improve the yield of rosemary fat-soluble extract, and
Because temperature is relatively low, reduce the amount that carnosic acid oxidation in fat-soluble extract is converted into carnosol, hence it is evident that improve product
Quality, and rosmarinic acid contents also have notable extraction in water-soluble extractive.With reference to produce reality, a step normal temperature height alcohol
Extraction method, under similarity condition, the energy loss in extraction and concentration process can be substantially reduced, production efficiency is improved, is being confused
Repeatedly there is great application value in terms of fragrant extract production.
Claims (2)
- A kind of 1. preparation method of Rosmarinus officinalis extract, it is characterised in that：Comprise the following steps：（1）Harvesting：The fresh rosemary leaf of harvesting is removed into stem, dries, is crushed to 30-80 mesh, lucifuge Cord blood is standby；（2）Extraction by steam distillation Rosemary Oil：By step（1）The rosemary crushed is added in round-bottomed flask, by material Liquor ratio 0.8-1.2:10 add pure water, distillation extraction 1-2h, and the liquid for distilling to obtain carries out water-oil separating with separatory funnel, received Collect the coarse-fine oil of rosemary, adding anhydrous sodium sulfate to the coarse-fine oil of rosemary is dehydrated, and filters to obtain finished product Rosemary Oil；（3）Normal temperature method extracts Rosmarinus officinalis extract：Step（2）Rosemary leaf slag pour into blowing-type drying machine, temperature is adjusted to 50-70 DEG C, 7-9h is dried, leaf slag is put into round-bottomed flask after drying, according to solid-liquid ratio 0.7-1.3:8 addition purity are 90%- 99% edible alcohol, it is placed on 45-65 DEG C of water-bath, heating extraction 1.5-2.5h, collects extract solution, similarity condition extracts again Once, extract solution twice is merged, just comprising rosemary water-soluble extractive and fat-soluble extract in the extract solution；（4）Concentration method separates Rosmarinus officinalis extract：By step（3）Middle extract solution is concentrated with vacuum and low temperature rotary evaporation instrument Recovered alcohol, when being concentrated into the 8%-13% that volume is extracting liquid volume, the extract solution after concentration is passed through Buchner funnel, adds water to enter Row filtering, the filter residue after filtering is poured into dryer, and temperature is adjusted to 50-65 DEG C, dries 7-9h, as rosemary is fat-soluble carries Take thing, when by the filtrate after filtering, to be concentrated into proportion be 1-1.5, be spray-dried both the water-soluble extraction of rosemary, pass through HLCP methods detect, and fat-soluble extract carnosic acid and carnosol content add up to 20%-25%, water-soluble extractive rosemary Acid content is 7%-9%.
- A kind of 2. preparation method of Rosmarinus officinalis extract according to claim 1, it is characterised in that：Comprise the following steps：（1）Harvesting：The fresh rosemary leaf of harvesting is removed into stem, dries, is crushed to 50 mesh, lucifuge Cord blood is standby；（2）Extraction by steam distillation Rosemary Oil：By step（1）The rosemary crushed is added in round-bottomed flask, by material Liquor ratio 1:10 add pure water, distillation extraction 1.5h, and the liquid for distilling to obtain carries out water-oil separating with separatory funnel, collects fan and change Fragrant coarse-fine oil, add anhydrous sodium sulfate to the coarse-fine oil of rosemary and be dehydrated, filter to obtain finished product Rosemary Oil；（3）Normal temperature method extracts Rosmarinus officinalis extract：Step（2）Rosemary leaf slag pour into blowing-type drying machine, temperature is adjusted to 60 DEG C, 8h is dried, leaf slag is put into round-bottomed flask after drying, according to solid-liquid ratio 1:8 addition purity are 95% edible alcohol, are put Put on 55 DEG C of water-baths, heating extraction 2h, collect extract solution, similarity condition extracts once again, merges extract solution twice, this is carried Take in liquid just comprising rosemary water-soluble extractive and fat-soluble extract；（4）Concentration method separates Rosmarinus officinalis extract：By step（3）Middle extract solution is concentrated with vacuum and low temperature rotary evaporation instrument Recovered alcohol, be concentrated into volume be extracting liquid volume 10% when, the extract solution after concentration is passed through Buchner funnel, adds water to carry out Filtering, the filter residue after filtering is poured into dryer, and temperature is adjusted to 60 DEG C, dries 8h, as rosemary fat-soluble extract, will Filtrate after filtering be concentrated into proportion for 1.2 when, be spray-dried both the water-soluble extraction of rosemary, examined by HLCP methods Survey, fat-soluble extract carnosic acid and carnosol content add up to 23.1%, and water-soluble extractive rosmarinic acid contents are 8.6%。
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109010426A (en) * | 2018-10-18 | 2018-12-18 | 武汉普丽缇莎科技有限责任公司 | A kind of manufacturing method of Rosmarinus officinalis extract |
| CN109161443A (en) * | 2017-11-13 | 2019-01-08 | 湖南诺泽生物科技有限公司 | A kind of fresh rosemary processing method |
| CN111440184A (en) * | 2018-01-30 | 2020-07-24 | 北京联合大学 | Method for preparing high-purity carnosol |
| CN113303458A (en) * | 2020-12-15 | 2021-08-27 | 广州百花香料股份有限公司 | Method for extracting curcuma extract |
| CN114916614A (en) * | 2022-05-27 | 2022-08-19 | 江西兴鼎科技有限公司 | Plant extract mixed feed additive with antioxidant activity |
| CN115282635A (en) * | 2022-08-03 | 2022-11-04 | 湖南植之源农牧有限公司 | Rosemary crude extract and preparation method and application thereof |
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| CN111440184B (en) * | 2018-01-30 | 2021-08-27 | 北京联合大学 | Method for preparing high-purity carnosol |
| CN109010426A (en) * | 2018-10-18 | 2018-12-18 | 武汉普丽缇莎科技有限责任公司 | A kind of manufacturing method of Rosmarinus officinalis extract |
| CN113303458A (en) * | 2020-12-15 | 2021-08-27 | 广州百花香料股份有限公司 | Method for extracting curcuma extract |
| CN114916614A (en) * | 2022-05-27 | 2022-08-19 | 江西兴鼎科技有限公司 | Plant extract mixed feed additive with antioxidant activity |
| CN114916614B (en) * | 2022-05-27 | 2023-08-29 | 江西兴鼎科技有限公司 | Plant extract mixed feed additive with antioxidant activity |
| CN115282635A (en) * | 2022-08-03 | 2022-11-04 | 湖南植之源农牧有限公司 | Rosemary crude extract and preparation method and application thereof |
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