CN105266075A - Method for extracting holothurian saponins by using holothurian blanching liquid - Google Patents
Method for extracting holothurian saponins by using holothurian blanching liquid Download PDFInfo
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- CN105266075A CN105266075A CN201510693837.3A CN201510693837A CN105266075A CN 105266075 A CN105266075 A CN 105266075A CN 201510693837 A CN201510693837 A CN 201510693837A CN 105266075 A CN105266075 A CN 105266075A
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- extract
- sea cucumber
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- holothurian
- selenka
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- 241000251511 Holothuroidea Species 0.000 title claims abstract description 56
- 238000000034 method Methods 0.000 title claims abstract description 43
- 206010033546 Pallor Diseases 0.000 title claims abstract description 36
- 239000007788 liquid Substances 0.000 title claims abstract description 20
- 229930182490 saponin Natural products 0.000 title abstract description 16
- 150000007949 saponins Chemical class 0.000 title abstract description 16
- 235000017709 saponins Nutrition 0.000 title abstract 3
- 239000000284 extract Substances 0.000 claims abstract description 41
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 33
- 239000000843 powder Substances 0.000 claims abstract description 22
- 239000011347 resin Substances 0.000 claims abstract description 22
- 229920005989 resin Polymers 0.000 claims abstract description 22
- 238000000967 suction filtration Methods 0.000 claims abstract description 21
- 238000000605 extraction Methods 0.000 claims abstract description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 20
- 239000012530 fluid Substances 0.000 claims abstract description 13
- 238000005377 adsorption chromatography Methods 0.000 claims abstract description 7
- 239000004519 grease Substances 0.000 claims abstract description 7
- 239000007787 solid Substances 0.000 claims abstract description 6
- 238000009777 vacuum freeze-drying Methods 0.000 claims abstract description 6
- 239000012141 concentrate Substances 0.000 claims description 32
- 238000010521 absorption reaction Methods 0.000 claims description 16
- 239000012065 filter cake Substances 0.000 claims description 15
- 239000011148 porous material Substances 0.000 claims description 13
- 239000003480 eluent Substances 0.000 claims description 11
- 238000005507 spraying Methods 0.000 claims description 11
- 239000006228 supernatant Substances 0.000 claims description 11
- 239000002994 raw material Substances 0.000 claims description 10
- 238000005238 degreasing Methods 0.000 claims description 6
- 238000001556 precipitation Methods 0.000 claims description 6
- 238000001042 affinity chromatography Methods 0.000 claims description 5
- 238000009835 boiling Methods 0.000 claims description 5
- 238000000151 deposition Methods 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 5
- 210000000936 intestine Anatomy 0.000 claims description 5
- 239000001397 quillaja saponaria molina bark Substances 0.000 abstract description 14
- 238000005516 engineering process Methods 0.000 abstract description 8
- 238000004519 manufacturing process Methods 0.000 abstract description 8
- 238000001179 sorption measurement Methods 0.000 abstract description 6
- 229930183167 cerebroside Natural products 0.000 abstract description 5
- 150000003904 phospholipids Chemical class 0.000 abstract description 4
- 238000010828 elution Methods 0.000 abstract description 3
- 150000001875 compounds Chemical class 0.000 abstract description 2
- 150000001784 cerebrosides Chemical class 0.000 abstract 1
- 238000010924 continuous production Methods 0.000 abstract 1
- 230000002035 prolonged effect Effects 0.000 abstract 1
- 238000001694 spray drying Methods 0.000 abstract 1
- JSPNNZKWADNWHI-PNANGNLXSA-N (2r)-2-hydroxy-n-[(2s,3r,4e,8e)-3-hydroxy-9-methyl-1-[(2r,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyoctadeca-4,8-dien-2-yl]heptadecanamide Chemical compound CCCCCCCCCCCCCCC[C@@H](O)C(=O)N[C@H]([C@H](O)\C=C\CC\C=C(/C)CCCCCCCCC)CO[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O JSPNNZKWADNWHI-PNANGNLXSA-N 0.000 description 4
- 239000002250 absorbent Substances 0.000 description 4
- 230000002745 absorbent Effects 0.000 description 4
- RIZIAUKTHDLMQX-UHFFFAOYSA-N cerebroside D Natural products CCCCCCCCCCCCCCCCC(O)C(=O)NC(C(O)C=CCCC=C(C)CCCCCCCCC)COC1OC(CO)C(O)C(O)C1O RIZIAUKTHDLMQX-UHFFFAOYSA-N 0.000 description 4
- 239000011149 active material Substances 0.000 description 3
- 229930182470 glycoside Natural products 0.000 description 3
- 150000002338 glycosides Chemical class 0.000 description 3
- 238000003754 machining Methods 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 238000003912 environmental pollution Methods 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 208000005189 Embolism Diseases 0.000 description 1
- 241000725303 Human immunodeficiency virus Species 0.000 description 1
- 206010029113 Neovascularisation Diseases 0.000 description 1
- 239000003463 adsorbent Substances 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000001857 anti-mycotic effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 239000002543 antimycotic Substances 0.000 description 1
- 206010003246 arthritis Diseases 0.000 description 1
- 230000002358 autolytic effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000020764 fibrinolysis Effects 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 208000002672 hepatitis B Diseases 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- -1 phosphatide Chemical class 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 230000017854 proteolysis Effects 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 238000003809 water extraction Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/616—Echinodermata, e.g. starfish, sea cucumbers or sea urchins
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Compounds Of Unknown Constitution (AREA)
- Preparation Of Fruits And Vegetables (AREA)
- Extraction Or Liquid Replacement (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a method for extracting holothurian saponins by using a holothurian blanching liquid. According to the method, the holothurian blanching liquid is subjected to concentration under reduced pressure and spray-drying and then subjected to extraction by using a supercritical CO2 fluid, solid powder with grease removed is obtained, subjected to alcohol extraction, suction filtration and concentration and then re-dissolved in water, compounds such as phospholipids, cerebrosides and the like in a solution are removed with a centrifugal method, then an obtained supernate is subjected to adsorption chromatography by the aid of a macroporous adsorption resin column, elution is performed by using an ethanol solution, an elution solution is subjected to vacuum freeze-drying, and a holothurian saponin extract is obtained. With the adoption of the method, the adsorption chromatography efficiency of the macroporous adsorption resin column is improved, the service life of the resin column is prolonged, the extracting method adopts the reasonable technology, operation is simple, the production cost is low, continuous production can be realized, and the purity and the yield of target products are increased.
Description
Technical field
The present invention relates to the technology extracting active material from Holothurian machining accessory substance, especially a kind of method utilizing sea cucumber blanching solution to extract selenka.
Background technology
After fishing for, there is the enzyme of proteolysis such as autolytic enzyme in fresh sea cucumber, being easy to skin, self-dissolving due to himself.For the ease of preserving, fresh sea cucumber will store through hot water scalding treatment aft-loaded airfoil usually.But fresh sea cucumber produces a large amount of blanching solutions in the process of hot water treatment, find after testing: containing a large amount of active materials in sea cucumber blanching solution, as selenka.And selenka is the class main active in sea cucumber, it has multiple pharmacologically active, as antitumor action, improves immunity, antimycotic, anti-neovascularization, promotion fibrinolysis, the formation of suppression embolism, antiinflammatory action (as arthritis), anti-radioactivity and suppresses AIDS virus, hepatitis B, HSV isolation etc.; Recently research finds that selenka also participates in fat metabolism, has effect of fat-reducing.
At present, utilize sea cucumber blanching solution to extract saponin(e and have bibliographical information.Such as, disclose in CN1733793A Chinese invention patent application prospectus disclosed in 15 days February in 2006 " a kind of process utilizing Holothurian machining waste liquid to prepare Sea Cucumber Glycosides ", first sea cucumber waste liquid filters by it, then filtrate alcohol settling, leave standstill, centrifugal, again centrifuged supernatant reduced pressure concentration is removed alcohol component, obtain concentrate, large pore resin absorption column is passed through with the flow velocity of 1.5 ~ 2.0BV/h, then use water with same flow velocity wash-out to remove salinity wherein, carbohydrate, the impurity components such as pigment, then the Sea Cucumber Glycosides composition on macroporous absorbent resin is attracted to 60 ~ 70% ethanol elutions, collect eluent and be evaporated to medicinal extract shape, freeze drying is Sea Cucumber Glycosides dry product.Though this process has simple to operate, with short production cycle, that production cost is low feature, but it directly utilizes macroporous absorbent resin to adsorb saponin(e in sea cucumber blanching solution, there is the deficiency that adsorption efficiency is low, absorption selectivity not by force, easily blocks up post, saponin content purity is low.
Summary of the invention
Directly utilize macroporous absorbent resin to adsorb saponin(e in sea cucumber blanching solution to have that adsorption efficiency is low to overcome in prior art, absorption selectivity not by force, easily stifled post, deficiency that saponin content purity is low, the invention provides that a kind of rational technology, operation feasible, production cost are low, efficiency of pcr product and the high sea cucumber blanching solution that utilizes of purity extract the method for selenka.
The technical solution adopted for the present invention to solve the technical problems is: a kind of method utilizing sea cucumber blanching solution to extract selenka, is characterized in that: it is through following process steps:
(1) the choosing and process of raw material
Choosing sea cucumber blanching solution is raw material, and by its reduced pressure concentration, being concentrated into solid content is 20 ~ 45%, obtains concentrate;
(2) spraying dry
By the concentrate spraying dry obtained in step (1), obtain the first pressed powder;
(3) degreasing
The first pressed powder obtained in step (2) is utilized supercritical CO
2fluid extraction, removes grease, obtains the second pressed powder;
(4) alcohol extracting
The second pressed powder obtained in step (3) is added the ethanolic solution of its quality 20 ~ 100 times, extract 12 ~ 24h, obtain extract; Wherein, the concentration of described ethanolic solution is 70 ~ 95%;
(5) suction filtration concentrates
The extract obtained in step (4) is carried out suction filtration, obtains suction filtration liquid; Filter cake is utilized again concentration be 70 ~ 95% ethanolic solution extract 12 ~ 24h, obtain filter cake alcohol extract; Repeat this step 2 time; Merge all suction filtration liquid and filter cake alcohol extract, reduced pressure concentration, remove ethanol, obtain concentrate;
(6) depositing in water
The pure water adding its volume 100 ~ 300 times in the concentrate obtained in step (5) is dissolved, removes precipitation through Horizontal helical type centrifuge, disc centrifuge, tube centrifuge successively, obtain supernatant, for subsequent use;
(7) affinity chromatography
By the supernatant that obtains in step (6) by large pore resin absorption column adsorption chromatography, utilize concentration be 60 ~ 70% ethanolic solution wash-out large pore resin absorption column, obtain eluent;
(8) concentrate drying
By the eluent vacuum freeze drying obtained in step (7), obtain selenka extract.
Described sea cucumber blanching solution is fresh sea cucumber after dissecting and removing intestines, puts into boiling water and boils 10 ~ 30min, pulls gained liquid after sea cucumber out.
Described supercritical CO
2the temperature of fluid extraction controls to be 40 ~ 50 DEG C, and pressure is 30 ~ 40Mpa, and extraction time is 1 ~ 2h.
Large pore resin absorption column is AB-8 type or D101 type.
Sea cucumber blanching solution concentrates by the present invention, and then spraying dry utilizes supercritical CO
2fluid extraction, the fluid of supercriticality has superpower solvability, is effectively stripped of the oil substances in sea cucumber blanching solution, avoids being mixed into of grease in subsequent extracted process; Sea cucumber blanching solution powder after degreasing, adopt alcohol precipitation water extraction, first utilize ethanolic solution to extract, multiple soluble in water again after extract is concentrated, adopt centrifugal method to remove the compound such as phosphatide, cerebroside in solution, reduce further again being mixed into of impurity in subsequent extracted process; Owing to have passed through the process of above-mentioned steps, the extraction solution obtained, clarity is high, improves macroporous adsorbent resin column chromatography adsorption efficiency, extends the service life of resin column, can be continuously produced, and improves the purity of object product; Extracted the selenka obtained by the method, purity is high, and total saponin content is between 90 ~ 95%.This technical method adds from Holothurian machining blanching solution, extract saponin(e operability and practicality, solve and directly utilize macroporous absorbent resin to adsorb saponin(e in sea cucumber blanching solution, adsorption efficiency is low, absorption selectivity not by force, easily stifled post, can not continuous seepage, drawback that saponin content purity is low.This extracting method rational technology, simple to operate, production cost is low, and the active material saponin(e of extraction all has higher health value, reduces the environmental pollution after the discharge of extra large blanching solution simultaneously, has Social and economic benef@widely.
Detailed description of the invention
below in conjunction with embodiment, the present invention will be further described.
embodiment 1
Utilize sea cucumber blanching solution to extract a method for selenka, it is through following process steps:
(1) the choosing and process of raw material
Choosing sea cucumber blanching solution is raw material, it is concentrated through 100 atmospheric pressure, and being concentrated into solid content is 30%, obtains concentrate; Wherein, sea cucumber blanching solution is fresh sea cucumber after dissecting and removing intestines, puts into boiling water and boils 20min, pulls gained liquid after sea cucumber out;
(2) spraying dry
By the concentrate spraying dry obtained in step (1), obtain the first pressed powder;
(3) degreasing
The first pressed powder obtained in step (2) is utilized supercritical CO
2fluid extraction, removes grease, obtains the second pressed powder; Wherein, supercritical CO
2the temperature of fluid extraction controls to be 45 DEG C, and pressure is 35Mpa, and extraction time is 1.5h;
(4) alcohol extracting
The second pressed powder obtained in step (3) is added the ethanolic solution of its quality 80 times, extract 15h, obtain extract; Wherein, the concentration of described ethanolic solution is 90%;
(5) suction filtration concentrates
The extract obtained in step (4) is carried out suction filtration, obtains suction filtration liquid; Filter cake is utilized again concentration be 90% ethanolic solution extract 15h, obtain filter cake alcohol extract; Repeat this step 2 time; Merge all suction filtration liquid and filter cake alcohol extract, reduced pressure concentration below 30 DEG C, remove ethanol, obtain concentrate;
(6) depositing in water
The pure water adding its volume 200 times in the concentrate obtained in step (5) is dissolved, successively through Horizontal helical type centrifuge (rotating speed 3000rpm), disc centrifuge (rotating speed 6000rpm), tube centrifuge (rotating speed 12000rpm), remove phospholipid, cerebroside precipitation, obtain supernatant, for subsequent use;
(7) affinity chromatography
By the supernatant that obtains in step (6) by AB-8 type large pore resin absorption column adsorption chromatography, utilize concentration be 65% ethanolic solution wash-out large pore resin absorption column, obtain eluent;
(8) concentrate drying
By the eluent vacuum freeze drying obtained in step (7), obtain selenka extract.
The extracting method rational technology that the present embodiment provides, simple to operate, production cost is low.Extracted the selenka obtained by the method, purity is high, after measured: total saponin content is 92%.Meanwhile, reduce the environmental pollution after the discharge of extra large blanching solution, there is Social and economic benef@widely.
embodiment 2
Utilize sea cucumber blanching solution to extract a method for selenka, it is through following process steps:
(1) the choosing and process of raw material
Choosing sea cucumber blanching solution is raw material, it is concentrated through 70 atmospheric pressure, and being concentrated into solid content is 45%, obtains concentrate; Wherein, sea cucumber blanching solution is fresh sea cucumber after dissecting and removing intestines, puts into boiling water and boils 30min, pulls gained liquid after sea cucumber out;
(2) spraying dry
By the concentrate spraying dry obtained in step (1), obtain the first pressed powder;
(3) degreasing
The first pressed powder obtained in step (2) is utilized supercritical CO
2fluid extraction, removes grease, obtains the second pressed powder; Wherein, supercritical CO
2the temperature of fluid extraction controls to be 50 DEG C, and pressure is 40Mpa, and extraction time is 2h;
(4) alcohol extracting
The second pressed powder obtained in step (3) is added the ethanolic solution of its quality 100 times, extract 24h, obtain extract; Wherein, the concentration of described ethanolic solution is 70%;
(5) suction filtration concentrates
The extract obtained in step (4) is carried out suction filtration, obtains suction filtration liquid; Filter cake is utilized again concentration be 70% ethanolic solution extract 24h, obtain filter cake alcohol extract; Repeat this step 2 time; Merge all suction filtration liquid and filter cake alcohol extract, reduced pressure concentration below 25 DEG C, remove ethanol, obtain concentrate;
(6) depositing in water
The pure water adding its volume 300 times in the concentrate obtained in step (5) is dissolved, through Horizontal helical type centrifuge (rotating speed 3000rpm), disc centrifuge (rotating speed 6000rpm), tube centrifuge (rotating speed 12000rpm), remove phospholipid, cerebroside precipitation, obtain supernatant, for subsequent use;
(7) affinity chromatography
By the supernatant that obtains in step (6) by D101 type large pore resin absorption column adsorption chromatography, utilize concentration be 70% ethanolic solution wash-out large pore resin absorption column, obtain eluent;
(8) concentrate drying
By the eluent vacuum freeze drying obtained in step (7), obtain selenka extract.
The extracting method rational technology that the present embodiment provides, simple to operate, production cost is low.Extracted the selenka obtained by the method, purity is high, after measured: total saponin content is 95%.
embodiment 3
Utilize sea cucumber blanching solution to extract a method for selenka, it is through following process steps:
(1) the choosing and process of raw material
Choosing sea cucumber blanching solution is raw material, it is concentrated through 200 atmospheric pressure, and being concentrated into solid content is 20%, obtains concentrate; Wherein, sea cucumber blanching solution is fresh sea cucumber after dissecting and removing intestines, puts into boiling water and boils 10min, pulls gained liquid after sea cucumber out;
(2) spraying dry
By the concentrate spraying dry obtained in step (1), obtain the first pressed powder;
(3) degreasing
The first pressed powder obtained in step (2) is utilized supercritical CO
2fluid extraction, removes grease, obtains the second pressed powder; Wherein, supercritical CO
2the temperature of fluid extraction controls to be 40 DEG C, and pressure is 30Mpa, and extraction time is 1h;
(4) alcohol extracting
The second pressed powder obtained in step (3) is added the ethanolic solution of its quality 20 times, extract 12h, obtain extract; Wherein, the concentration of described ethanolic solution is 95%;
(5) suction filtration concentrates
The extract obtained in step (4) is carried out suction filtration, obtains suction filtration liquid; Filter cake is utilized again concentration be 95% ethanolic solution extract 12h, obtain filter cake alcohol extract; Repeat this step 2 time; Merge all suction filtration liquid and filter cake alcohol extract, reduced pressure concentration below 30 DEG C, remove ethanol, obtain concentrate;
(6) depositing in water
The pure water adding its volume 100 times in the concentrate obtained in step (5) is dissolved, through Horizontal helical type centrifuge (rotating speed 3000rpm), disc centrifuge (rotating speed 6000rpm), tube centrifuge (rotating speed 12000rpm), remove phospholipid, cerebroside precipitation, obtain supernatant, for subsequent use;
(7) affinity chromatography
By the supernatant that obtains in step (6) by AB-8 type large pore resin absorption column adsorption chromatography, utilize concentration be 60% ethanolic solution wash-out large pore resin absorption column, obtain eluent;
(8) concentrate drying
By the eluent vacuum freeze drying obtained in step (7), obtain selenka extract.
The extracting method rational technology that the present embodiment provides, simple to operate, production cost is low.Extracted the selenka obtained by the method, purity is high, after measured: total saponin content is 90%.
Claims (4)
1. utilize sea cucumber blanching solution to extract a method for selenka, it is characterized in that: it is through following process steps:
(1) the choosing and process of raw material
Choosing sea cucumber blanching solution is raw material, and by its reduced pressure concentration, being concentrated into solid content is 20 ~ 45%, obtains concentrate;
(2) spraying dry
By the concentrate spraying dry obtained in step (1), obtain the first pressed powder;
(3) degreasing
The first pressed powder obtained in step (2) is utilized supercritical CO
2fluid extraction, removes grease, obtains the second pressed powder;
(4) alcohol extracting
The second pressed powder obtained in step (3) is added the ethanolic solution of its quality 20 ~ 100 times, extract 12 ~ 24h, obtain extract; Wherein, the concentration of described ethanolic solution is 70 ~ 95%;
(5) suction filtration concentrates
The extract obtained in step (4) is carried out suction filtration, obtains suction filtration liquid; Filter cake is utilized again concentration be 70 ~ 95% ethanolic solution extract 12 ~ 24h, obtain filter cake alcohol extract; Repeat this step 2 time; Merge all suction filtration liquid and filter cake alcohol extract, reduced pressure concentration, remove ethanol, obtain concentrate;
(6) depositing in water
The pure water adding its volume 100 ~ 300 times in the concentrate obtained in step (5) is dissolved, removes precipitation through Horizontal helical type centrifuge, disc centrifuge, tube centrifuge successively, obtain supernatant, for subsequent use;
(7) affinity chromatography
By the supernatant that obtains in step (6) by large pore resin absorption column adsorption chromatography, utilize concentration be 60 ~ 70% ethanolic solution wash-out large pore resin absorption column, obtain eluent;
(8) concentrate drying
By the eluent vacuum freeze drying obtained in step (7), obtain selenka extract.
2. a kind of sea cucumber blanching solution that utilizes according to claim 1 extracts the method for selenka, it is characterized in that: described sea cucumber blanching solution be fresh sea cucumber after dissecting and removing intestines, put into boiling water and boil 10 ~ 30min, pull gained liquid after sea cucumber out.
3. a kind of method utilizing sea cucumber blanching solution to extract selenka according to claim 1, is characterized in that: described supercritical CO
2the temperature of fluid extraction controls to be 40 ~ 50 DEG C, and pressure is 30 ~ 40Mpa, and extraction time is 1 ~ 2h.
4. a kind of method utilizing sea cucumber blanching solution to extract selenka according to claim 1, is characterized in that: large pore resin absorption column is AB-8 type or D101 type.
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| CN201510693837.3A CN105266075B (en) | 2015-10-24 | 2015-10-24 | method for extracting Stichopus japonicus saponins from Stichopus japonicus blanching liquid |
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| CN201510693837.3A CN105266075B (en) | 2015-10-24 | 2015-10-24 | method for extracting Stichopus japonicus saponins from Stichopus japonicus blanching liquid |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106038602A (en) * | 2016-06-23 | 2016-10-26 | 蓬莱海洋（山东）股份有限公司 | Method for extracting high-purity visceral saponins of sea cucumber by using sea cucumber viscera |
| CN110256524A (en) * | 2019-07-19 | 2019-09-20 | 荣成市慧海创达生物科技有限公司 | The extracting method of selenka |
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| CN104402983A (en) * | 2014-12-11 | 2015-03-11 | 山东省科学院生物研究所 | Method for extracting glycoprotein by utilizing sea cucumber treatment blanching liquid and application thereof |
| CN104739868A (en) * | 2015-02-15 | 2015-07-01 | 大连工业大学 | Comprehensive utilization method for sea cucumber processing waste liquid |
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2015
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| CN104262451A (en) * | 2014-10-24 | 2015-01-07 | 威海芝恩药业股份有限公司 | Method for extracting holothurin from sea cucumber processing waste fluid |
| CN104402983A (en) * | 2014-12-11 | 2015-03-11 | 山东省科学院生物研究所 | Method for extracting glycoprotein by utilizing sea cucumber treatment blanching liquid and application thereof |
| CN104739868A (en) * | 2015-02-15 | 2015-07-01 | 大连工业大学 | Comprehensive utilization method for sea cucumber processing waste liquid |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106038602A (en) * | 2016-06-23 | 2016-10-26 | 蓬莱海洋（山东）股份有限公司 | Method for extracting high-purity visceral saponins of sea cucumber by using sea cucumber viscera |
| CN106038602B (en) * | 2016-06-23 | 2019-08-16 | 蓬莱海洋（山东）股份有限公司 | A method of high-purity sea cucumber internal organ saponin(e is extracted using sea cucumber internal organ |
| CN110256524A (en) * | 2019-07-19 | 2019-09-20 | 荣成市慧海创达生物科技有限公司 | The extracting method of selenka |
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| CN105266075B (en) | 2020-01-31 |
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