CN107475073A - A kind of cordyceps species culture systems - Google Patents
A kind of cordyceps species culture systems Download PDFInfo
- Publication number
- CN107475073A CN107475073A CN201710778519.6A CN201710778519A CN107475073A CN 107475073 A CN107475073 A CN 107475073A CN 201710778519 A CN201710778519 A CN 201710778519A CN 107475073 A CN107475073 A CN 107475073A
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- round
- culture systems
- fermentation
- filter assemblies
- cordyceps species
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Links
- 241000190633 Cordyceps Species 0.000 title claims abstract description 22
- 230000000712 assembly Effects 0.000 claims abstract description 37
- 238000000429 assembly Methods 0.000 claims abstract description 37
- 238000000855 fermentation Methods 0.000 claims abstract description 33
- 230000004151 fermentation Effects 0.000 claims abstract description 33
- 241000894006 Bacteria Species 0.000 claims abstract description 26
- 230000007246 mechanism Effects 0.000 claims abstract description 23
- 238000001914 filtration Methods 0.000 claims abstract description 18
- 235000015097 nutrients Nutrition 0.000 claims abstract description 17
- 230000001954 sterilising effect Effects 0.000 claims description 38
- 238000004659 sterilization and disinfection Methods 0.000 claims description 14
- 239000000110 cooling liquid Substances 0.000 claims description 11
- 238000010438 heat treatment Methods 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 6
- 238000007599 discharging Methods 0.000 claims description 2
- 238000010276 construction Methods 0.000 abstract description 2
- 238000001816 cooling Methods 0.000 description 5
- 230000006835 compression Effects 0.000 description 2
- 238000007906 compression Methods 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 125000003158 alcohol group Chemical group 0.000 description 1
- 238000010923 batch production Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000002485 combustion reaction Methods 0.000 description 1
- 239000002826 coolant Substances 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000009655 industrial fermentation Methods 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000010025 steaming Methods 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/22—Transparent or translucent parts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M39/00—Means for cleaning the apparatus or avoiding unwanted deposits of microorganisms
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- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a kind of cordyceps species culture systems, including：Filter mechanism, filter mechanism include the filter assemblies of at least one set first for filtering compressed air；Ferment mechanism, fermentation mechanism include be used for place strain and nutrient solution round, on round be used for be put into strain enter bacterium mouth and for export fermentation complete strain go out bacterium mouth, on round be used for be passed through the first filter assemblies filtering after compressed air air inlet and for pass-out ferment after compressed air gas outlet.A kind of cordyceps species culture systems of the present invention, by the setting of filter mechanism and fermentation mechanism, the yield of cordyceps species is greatly improved, and it is simple in construction, it is easy to operate.
Description
Technical field
The present invention relates to Spawn incubation field, and in particular to a kind of cordyceps species culture systems.
Background technology
In the prior art, cordyceps species are cultivated to common are using shaking table the shaking culture that produces and using fermentation tank
Come the submerged-culture method produced, if a small amount of production can use shaking culture, deep drainpipe needs a whole set of industrial fermentation to set
Standby, investment is big, is only applicable to the large-scale production of batch production.
The content of the invention
It is an object of the invention to provide a kind of cordyceps species culture systems simple in construction, easy to operate and high yield.
To reach above-mentioned purpose, the technical solution adopted by the present invention is：
A kind of cordyceps species culture systems, including：
Filter mechanism, the filter mechanism include the filter assemblies of at least one set first for filtering compressed air；
Fermentation mechanism, the fermentation mechanism include being used to place the round of strain and nutrient solution, located at the round
On be used for be put into entering bacterium mouth and going out bacterium mouth, on the round for exporting the strain that fermentation is completed for strain
For the pressure after being passed through the air inlet of the compressed air after first filter assemblies filter and being fermented for pass-out
The gas outlet of contracting air；
First filter assemblies are connected with the round by pipeline.
Preferably, the filter mechanism also includes being located at being used between first filter assemblies and the round
The second filter assemblies that compressed air after the filtering is further filtered.
Preferably, first filter assemblies have three groups, and the first filter assemblies described in three groups press filtering accuracy from low to high
It is sequentially communicated.
Preferably, the culture systems also include being used for controlling first filter assemblies and the round it
Between the pipeline opening and closing the first control valve.
Preferably, it is described enter bacterium mouth on the outside of all portions be equipped with sterilizing groove for sterilizing, filled with can in the sterilizing groove
Combustion things matter.
Preferably, be additionally provided with the heating gate for being passed through high-temperature steam on the round, the air inlet, it is described go out
Gas port, it is described go out bacterium mouth on be communicated with flexible pipe, the air inlet is connected by the flexible pipe with first filter assemblies, institute
Gas outlet is stated by the compressed air after the fermentation of the flexible pipe outside pass-out, it is described go out bacterium mouth pass through flexible pipe output hair
The strain that ferment is completed, it is dismountable on the round to be provided with the sterilizing component for being used for that high-temperature sterilization to be carried out to the flexible pipe.
It is further preferred that the sterilizing component includes being used for placing the hollow sterilization chamber of the flexible pipe, folding is set
In the sterilizing lid in the sterilization chamber, the sterilizing is covered provided with for discharging the air vent of the high-temperature steam, for controlling
Second control valve of the air vent opening and closing.
Preferably, the round includes the inner pressurd vessel for placing the nutrient solution and is sheathed on the inner pressurd vessel
Outer container, the outer container be provided be used for be passed through cooling liquid inlet and go out liquid for cooling liquid described in pass-out
Mouthful, gap is provided between the inner pressurd vessel outer surface and the outer container inner surface, the cooling liquid flows in the gap
Move and exchanged heat with the nutrient solution.
Preferably, the round is provided with the thermometer hole for being used for measuring temperature in the round.
Preferably, the observation for observing strain fermentation situation in the round is additionally provided with the round
Window.
Due to the utilization of above-mentioned technical proposal, the present invention has following advantages compared with prior art：One kind of the present invention
Cordyceps species culture systems, by the setting of filter mechanism and fermentation mechanism, the yield of cordyceps species is greatly improved, and tied
Structure is simple, easy to operate.
Brief description of the drawings
Accompanying drawing 1 is schematic diagram of the invention；
Accompanying drawing 2 is the structural representation of the round of the present invention.
Wherein：1st, the first filter assemblies；2nd, round；3rd, bacterium mouth is entered；4th, bacterium mouth is gone out；5th, air inlet；6th, gas outlet；7、
Second filter assemblies；8th, the first control valve；9th, sterilize groove；10th, heating gate；11st, flexible pipe；12nd, sterilization chamber；13rd, sterilizing lid；14、
Air vent；15th, the second control valve；16th, inlet；17th, liquid outlet；18th, gap；19th, thermometer hole；20th, observation window.
Embodiment
The technical solution of the present invention will be further described below with reference to the accompanying drawings.
Referring to shown in Fig. 1-2, a kind of cordyceps species culture systems, including：
Filter mechanism, filter mechanism include the first filter assemblies of at least one set 1 for filtering compressed air.In the present embodiment
In, compressed air is compressed by air compressor machine, and motor driving air compressor machine suction outside air is simultaneously compressed, after compression
Air input the first filter assemblies 1 in filtered, to reach the requirement of strain fermentation.First filter assemblies 1 have three groups, and three
The first filter assemblies 1 of group are sequentially communicated from low to high by filtering accuracy.The filter core model of three group of first filter assemblies 1 is followed successively by
E5-20、E7-20、E9-20。
Fermentation mechanism, fermentation mechanism include being used to place the round 2 of strain and nutrient solution, located at round 2
Be used for be put into entering bacterium mouth 3 and going out bacterium mouth 4, the use on round 2 for exporting the strain that fermentation is completed for strain
In be passed through the first filter assemblies 1 filtering after compressed air air inlet 5 and for pass-out ferment after compressed air outlet
Mouth 6, the first filter assemblies 1 are connected with round 2 by pipeline.
In the present embodiment, round 2 is big hollow fermentation tank, and first nutrient solution is added before strain is put into
Heat, influence the bacterium of strain fermentation to kill the possibility in nutrient solution.It is additionally provided with round 2 for being passed through high-temperature steam
Heating gate 10, high-temperature steam is produced by a small steam generator, then self-heating mouth 10 is passed through in round 2,
Nutrient solution is heated.Heating setpoint temperature is 121 DEG C.It is provided with round 2 and is used to measure temperature in round 2
Thermometer hole 19, in thermometer hole 19 inserting thermometer measures the internal temperature of round 2, stops at reach setting 121 DEG C
Only it is passed through high-temperature steam.
Fermented after reaching design temperature, it is necessary to make Solution cooling to be just passed through strain to assigned temperature, the cooling
Temperature is 23 DEG C, and herein, round 2 includes being used for the inner pressurd vessel for placing nutrient solution and the outer appearance being sheathed on inner pressurd vessel
Device, outer container, which is provided with, is used for the inlet 16 for being passed through cooling liquid and the liquid outlet 17 for pass-out cooling liquid, inner pressurd vessel
Gap 18 is provided between outer surface and outer container inner surface, cooling liquid flows in gap 18 and exchanged heat with nutrient solution,
When Solution cooling is to 23 DEG C, cooling liquid is extracted out from liquid outlet 17.
Strain is added after Solution cooling, enters all portions on the outside of bacterium mouth 3 and is equipped with the sterilizing groove 9 for sterilizing, sterilize groove 9
It is interior to be filled with combustible.Herein, combustible is alcohol, and when adding strain, alcohol is lighted, is entering shape at bacterium mouth 3
Into flame sealing ring, it is ensured that will not be infected when adding strain influences other bacteriums of strain survival.
Filter mechanism also includes being used for the compression sky after filtering located between the first filter assemblies 1 and round 2
The second filter assemblies 7 that gas further filters.Second filter assemblies 7 are small-sized water filter, will pass through the first filtering group
Compressed air after part 1 filters further is filtered, and more ensure that the survival rate of strain.
Culture systems also include being used to control the first control of the pipeline opening and closing between the first filter assemblies 1 and round 2
Valve 8 processed.The compressed air being passed through after filtering, set duration of ventilation as 48 hours, after reaching setting time, closed the first control valve
8, stop the compressed air after input filtering into round 2.Herein, the pipeline of the pass-out of the first filter assemblies 1 can be divided into
Multistage, multistage pipeline set multiple first control valves 8, and multistage pipeline can be passed through compressed air into multiple rounds 2 simultaneously,
Substantially increase the yield of strain.
In air inlet 5, gas outlet 6, go out to be communicated with flexible pipe 11 on bacterium mouth 4, air inlet 5 is filtered by flexible pipe 11 and first
Component 1 connects, and gas outlet 6 goes out bacterium mouth 4 and pass through the output fermentation of flexible pipe 11 by the compressed air after the fermentation of 11 outside pass-out of flexible pipe
The strain of completion, it is dismountable on round 2 to be provided with the sterilizing component for being used for being sterilized to flexible pipe 11.
Sterilizing component includes being used for hollow sterilization chamber 12, the sterilizing in sterilization chamber 12 of folding for placing flexible pipe 11
Lid 13, sterilizing lid 13 are provided with the air vent 14 for being used to discharge high-temperature steam, the second control for controlling air vent 14 to be opened and closed
Valve 15.
In the pipeline delivery outlet of the pass-out of the first filter assemblies 1, it is provided with flexible pipe 11 and is connected with air inlet 5, gas outlet 6 passes through
Compressed air after the fermentation of 11 outside pass-out of flexible pipe, goes out the strain that bacterium mouth 4 exports fermentation completion by flexible pipe 11, and flexible pipe 11 is set
Put, can flexibly dismount, it is more convenient.It is to ensure the sterile of environment simultaneously, it is necessary to carry out high-temperature sterilization to flexible pipe 11, therefore
Sterilizing component is set on round 2, and the sterilizing component is sterilized by heating the high-temperature steam of nutrient solution.High temperature steams
Vapour self-heating mouth 10 is passed through in round 2, and a part is used to heat nutrient solution, another part continue on be advanced into sterilization chamber 12
Flexible pipe 11 is sterilized.Herein, air inlet 5, gas outlet 6, go out bacterium mouth 4 and be packaged in sterilization chamber 12, therefore can be
Flexible pipe 11 is directly sterilized when connection.The lid 13 that sterilized during sterilizing needs sealed set with sterilization chamber 12, and high-temperature steam is passed through
High-temperature sterilization is carried out to flexible pipe 11, when reaching air pressure critical value, the second control valve 15 is opened, and air vent 14 is outwards deflated, with height
Press the principle of pot identical.
The observation window 20 for observing strain fermentation situation in round 2 is additionally provided with round 2.Fermentation process
In can observe fermentation situation at any time, fermentation process is adjusted according to different situations.
The course of work of lower the present embodiment is illustrated in detail below：High temperature steaming is passed through into round 2 first from air inlet 5
Vapour, the nutrient solution in round 2 is heated, coolant is passed through into round 2 from inlet 16 after the completion of heating
Body, nutrient solution is cooled down, cooling liquid is extracted out from liquid outlet 17 after the completion of cooling, then from the addition strain of bacterium mouth 3 is entered, together
When compressed air is filtered by the first filter assemblies 1 and the second filter assemblies 7, the compressed air after filtering is passed through
Fermented in round 2, fermentation is completed to take out bacterium solution.
The above embodiments merely illustrate the technical concept and features of the present invention, and its object is to allow person skilled in the art
Scholar can understand present disclosure and be carried out, and it is not intended to limit the scope of the present invention, all according to the present invention
The equivalent change or modification that Spirit Essence is made, it should all cover within the scope of the present invention.
Claims (10)
- A kind of 1. cordyceps species culture systems, it is characterised in that：Including：Filter mechanism, the filter mechanism include the filter assemblies of at least one set first for filtering compressed air（1）；Fermentation mechanism, the fermentation mechanism include being used for the round for placing strain and nutrient solution（2）, located at it is described fermentation hold Device（2）On be used to being put into strain enter bacterium mouth（3）Go out bacterium mouth with for exporting the strain that fermentation is completed（4）, located at described Round（2）On be used for be passed through first filter assemblies（1）The air inlet of the compressed air after filtering（5）With with The gas outlet of the compressed air after pass-out fermentation（6）；First filter assemblies（1）With the round（2）Connected by pipeline.
- A kind of 2. cordyceps species culture systems according to claim 1, it is characterised in that：The filter mechanism also includes setting In first filter assemblies（1）With the round（2）Between be used for by the compressed air after the filtering it is further Second filter assemblies of filtering（7）.
- A kind of 3. cordyceps species culture systems according to claim 1, it is characterised in that：First filter assemblies（1） There are three groups, the first filter assemblies described in three groups（1）It is sequentially communicated from low to high by filtering accuracy.
- A kind of 4. cordyceps species culture systems according to claim 1, it is characterised in that：The culture systems also include using In control first filter assemblies（1）With the round（2）Between the pipeline opening and closing the first control valve （8）.
- A kind of 5. cordyceps species culture systems according to claim 1, it is characterised in that：It is described enter bacterium mouth（3）Outside week Portion is equipped with the sterilizing groove for sterilizing（9）, the sterilizing groove（9）It is interior to be filled with combustible.
- A kind of 6. cordyceps species culture systems according to claim 1, it is characterised in that：The round（2）It is upper to go back Provided with the heating gate for being passed through high-temperature steam（10）, the air inlet（5）, the gas outlet（6）, it is described go out bacterium mouth（4）On It is communicated with flexible pipe（11）, the air inlet（5）Pass through the flexible pipe（11）With first filter assemblies（1）Connection, it is described Gas outlet（6）Pass through the flexible pipe（11）The compressed air after outside pass-out fermentation, it is described go out bacterium mouth（4）By described soft Pipe（11）The strain that output fermentation is completed, the round（2）Upper dismountable be provided with is used for the flexible pipe（11）Carry out The sterilizing component of high-temperature sterilization.
- A kind of 7. cordyceps species culture systems according to claim 6, it is characterised in that：The sterilizing component includes being used for Place the flexible pipe（11）Hollow sterilization chamber（12）, folding be located at the sterilization chamber（12）On sterilizing lid（13）, institute State sterilizing lid（13）It is provided with the air vent for being used for discharging the high-temperature steam（14）, for controlling the air vent（14）Opening and closing The second control valve（15）.
- A kind of 8. cordyceps species culture systems according to claim 1, it is characterised in that：The round（2）Including For the inner pressurd vessel for placing the nutrient solution and the outer container being sheathed on the inner pressurd vessel, the outer container, which is provided with, to be used to lead to Enter the inlet of cooling liquid（16）With the liquid outlet for cooling liquid described in pass-out（17）, the inner pressurd vessel outer surface and institute State and be provided with gap between outer container inner surface（18）, the cooling liquid is in the gap（18）It is middle flowing and with the nutrient solution Exchanged heat.
- A kind of 9. cordyceps species culture systems according to claim 1, it is characterised in that：The round（2）On set Have for measuring the round（2）The thermometer hole of interior temperature（19）.
- A kind of 10. cordyceps species culture systems according to claim 1, it is characterised in that：The round（2）It is upper to go back Provided with for observing the round（2）The observation window of interior strain fermentation situation（20）.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710778519.6A CN107475073A (en) | 2017-09-01 | 2017-09-01 | A kind of cordyceps species culture systems |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710778519.6A CN107475073A (en) | 2017-09-01 | 2017-09-01 | A kind of cordyceps species culture systems |
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| Publication Number | Publication Date |
|---|---|
| CN107475073A true CN107475073A (en) | 2017-12-15 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710778519.6A Pending CN107475073A (en) | 2017-09-01 | 2017-09-01 | A kind of cordyceps species culture systems |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4324762A (en) * | 1979-07-03 | 1982-04-13 | Redikultsev Jury V | Apparatus for sterilization by steam of fermentation objects |
| CN101353618A (en) * | 2007-07-25 | 2009-01-28 | 上海市农业科学院 | Production method of edible fungus liquid spawn and fermentation plant thereof |
| CN202077430U (en) * | 2011-05-24 | 2011-12-21 | 湖北启明生物工程有限公司 | Fermentation tank with sterilizer at outer part |
| CN102895685A (en) * | 2012-09-11 | 2013-01-30 | 北京中怡远大机电设备有限公司 | Sterilization system for medium, sterilization method using same, and culture method for cordyceps militaris |
| CN207121599U (en) * | 2017-09-01 | 2018-03-20 | 苏州顺泰元虫草生物科技有限公司 | A kind of cordyceps species culture systems |
-
2017
- 2017-09-01 CN CN201710778519.6A patent/CN107475073A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4324762A (en) * | 1979-07-03 | 1982-04-13 | Redikultsev Jury V | Apparatus for sterilization by steam of fermentation objects |
| CN101353618A (en) * | 2007-07-25 | 2009-01-28 | 上海市农业科学院 | Production method of edible fungus liquid spawn and fermentation plant thereof |
| CN202077430U (en) * | 2011-05-24 | 2011-12-21 | 湖北启明生物工程有限公司 | Fermentation tank with sterilizer at outer part |
| CN102895685A (en) * | 2012-09-11 | 2013-01-30 | 北京中怡远大机电设备有限公司 | Sterilization system for medium, sterilization method using same, and culture method for cordyceps militaris |
| CN207121599U (en) * | 2017-09-01 | 2018-03-20 | 苏州顺泰元虫草生物科技有限公司 | A kind of cordyceps species culture systems |
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Application publication date: 20171215 |
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