CN107468955B - Traditional Chinese medicine for treating gouty arthritis and preparation method - Google Patents

Abstract

The invention discloses a traditional Chinese medicine for treating gouty arthritis, which is composed of volatile oil and water extract of Chinese herbal medicines, wherein the Chinese herbal medicines comprise the following components in percentage by mass: 10 parts of golden cypress, 10 parts of rhizoma atractylodis, 30 parts of rhizoma smilacis glabrae, 10 parts of liquorice, 15 parts of radix clematidis, 10 parts of radix paeoniae rubra, 15 parts of polygonum cuspidatum, 10 parts of angelica sinensis, 8 parts of Indian iphigenia bulb, 10 parts of radix angelicae pubescentis, 15 parts of codonopsis pilosula and 10. Pharmacological experiment results show that the traditional Chinese medicine composition has a good effect of treating gouty arthritis, can improve the curative effects of joint swelling, inflammatory factor change, antioxidant factor change and the like caused by gouty arthritis, can be prepared into various dosage form medicines with pharmaceutically acceptable carriers, and is convenient for clinical administration. The preparation method provided by the invention has the advantages of strong operability, low cost, environmental protection and realization of industrial mass production.

Description

Traditional Chinese medicine for treating gouty arthritis and preparation method

Technical Field

The invention provides a medicament for treating arthritis and a preparation method thereof, in particular to a traditional Chinese medicine for treating gouty arthritis and a preparation method thereof, wherein the traditional Chinese medicine has reliable curative effect and small side effect.

Background

Gouty arthritis is a disease mainly caused by joint pain, swelling and deformity due to purine metabolic disorder, hyperuricemia or excretion disorder, and increased blood uric acid concentration and urate deposition. Has the characteristics of acute and rapid onset of disease, severe pain, difficult recovery from prolonged period and the like, and has seriously influenced the health and daily life of people. Colchicine is one of effective drugs for treating gout, especially severe acute attack, but colchicine can not inhibit the generation of uric acid nor promote the excretion of uric acid. Diarrhea, vomiting, abdominal cramps are common adverse effects and are the first indicator of toxicity, and therapeutically effective doses are close to those which cause gastrointestinal symptoms. The non-steroidal anti-inflammatory drugs can not only cause gastrointestinal toxicity, but also cause adverse reactions to the kidney, the central nervous system, the blood system, the cardiovascular system, the fetus and the skin, and two or more of the drugs cannot be taken simultaneously. When the glucocorticoid is used for treating gout, the phenomenon of 'rebound' of symptoms is easy to occur after the medicine withdrawal. The only drug for inhibiting uric acid production on the market at present is allopurinol, which is suitable for hyperuricemia but has adverse reactions such as liver and bone marrow toxicity and allergy. The drugs for promoting uric acid excretion such as probenecid, metconazole, benzbromarone and the like mainly promote uric acid excretion by inhibiting reabsorption of uric acid by a proximal renal tubule, and have the greatest defect of causing deposition of urate crystals in a urinary tract to cause renal colic and renal function damage. In conclusion, the medicines and biological preparations used for treating gouty arthritis in western medicine are difficult to popularize or use for a long time due to unsatisfactory curative effect or large toxic and side effect.

Disclosure of Invention

The invention aims to solve the technical problems in the prior art and provides a traditional Chinese medicine for treating gouty arthritis and a preparation method thereof, wherein the traditional Chinese medicine has reliable curative effect and small side effect.

The technical solution of the invention is as follows: the traditional Chinese medicine for treating gouty arthritis is characterized by comprising volatile oil and water extract of Chinese herbal medicines, wherein the Chinese herbal medicines comprise the following components in percentage by mass: 10 parts of golden cypress, 10 parts of rhizoma atractylodis, 30 parts of rhizoma smilacis glabrae, 10 parts of liquorice, 15 parts of radix clematidis, 10 parts of radix paeoniae rubra, 15 parts of polygonum cuspidatum, 10 parts of angelica sinensis, 8 parts of Indian iphigenia bulb, 10 parts of radix angelicae pubescentis, 15 parts of codonopsis pilosula and 10.

The preparation method of the medicine for treating gouty arthritis is characterized by comprising the following steps:

a. mixing cortex Phellodendri, rhizoma Atractylodis, rhizoma Smilacis Glabrae, Glycyrrhrizae radix, radix Clematidis, radix Paeoniae Rubra, rhizoma Polygoni Cuspidati, radix Angelicae sinensis, Pseudobulbus Cremastrae Seu pleiones, radix Angelicae Pubescentis, radix Codonopsis and radix et rhizoma Rhei, pulverizing, placing into a round bottom flask, adding 8 times of purified water, shaking, mixing, and soaking for 30 min;

b. connecting a round-bottom flask with a volatile oil extractor and a reflux condenser tube, adding water from the upper end of the condenser tube until the upper end of the condenser tube is filled with the scale part of the volatile oil detector and overflows into the round-bottom flask, then placing the round-bottom flask in an electric heating jacket to slowly heat the water until the water is boiled and keeps slightly boiling for 6 hours, stopping heating, placing and cooling, starting a piston at the lower end of the volatile oil extractor to slowly discharge the water, and collecting the volatile oil for later use;

c. b, taking the dregs of the decoction after the volatile oil is extracted in the step b, adding 8 times of purified water to decoct for 2 times, each time for 2 hours, filtering, combining the filtrates, and concentrating under reduced pressure to obtain a water extract;

d. mixing the volatile oil and water extract.

The invention is prepared by Chinese herbal medicines with small side effect, the proportion of each component is scientific and reasonable, and the volatile oil and the water extract play the effect together. Pharmacological experiment results show that the traditional Chinese medicine composition has a good effect of treating gouty arthritis, can improve the curative effects of joint swelling, inflammatory factor change, antioxidant factor change and the like caused by gouty arthritis, can be prepared into various dosage form medicines with pharmaceutically acceptable carriers, and is convenient for clinical administration. The preparation method provided by the invention has the advantages of strong operability, low cost, environmental protection and realization of industrial mass production.

Detailed Description

a. Mixing and crushing 10g of golden cypress, 10g of rhizoma atractylodis, 30g of rhizoma smilacis glabrae, 10g of liquorice, 15g of radix clematidis, 10g of radix paeoniae rubra, 15g of polygonum cuspidatum, 10g of angelica, 8g of Indian iphigenia bulb, 10g of radix angelicae pubescentis, 15g of codonopsis pilosula and 10g of wine-treated rhubarb, putting the mixture into a round-bottomed flask, adding purified water which is 8 times of the total raw materials, shaking and mixing the mixture;

b. connecting a round-bottom flask with a volatile oil extractor and a reflux condenser tube, adding water from the upper end of the condenser tube until the upper end of the condenser tube is filled with the scale part of the volatile oil detector and overflows into the round-bottom flask, then placing the round-bottom flask in an electric heating jacket to slowly heat the water until the water is boiled and keeps slightly boiling for 6 hours, stopping heating, placing and cooling, starting a piston at the lower end of the volatile oil extractor to slowly discharge the water, and collecting the volatile oil for later use;

c. b, taking the dregs of the decoction after the volatile oil is extracted in the step b, adding 8 times of purified water to decoct for 2 times, each time for 2 hours, filtering, combining the filtrates, and concentrating under reduced pressure to obtain a water extract;

d. mixing the volatile oil and water extract.

Can be prepared into various dosage forms with pharmaceutically acceptable carriers

Experiment:

1. experiment grouping

The 60 rats were randomly divided into 6 groups, 10 GA groups, 10 colchicine groups, 10 blank control (NS) groups, 10 low dose groups according to the present example, 10 dose groups according to the present example, and 10 high dose groups according to the present example.

1. Experimental procedures

50 mul of 5 percent sodium urate solution is injected into the tibiotarsal joint cavity of the right hind ankle of each group of rats to establish a rat gouty arthritis model. The circumference of the rat at 0.5mm below the right hind ankle joint was measured before molding as a 0h measurement and the drug was administered by gavage separately for 8 days after molding. The blank control (NS) group is given with physiological saline, the colchicine group is given according to 1.5mg/kg, the low dose group, the medium dose group and the high dose group of the embodiment of the invention are respectively given with three concentrations of 1.5g/kg, 3g/kg and 6g/kg of 0.5 percent CMC-Na dissolved solvent, and are given twice a day according to the following dose volumes: 1ml/100 g. Measuring the peripheral length of the right hind ankle joint of the rat at 0h, 24h, 48h, 72h, 96h, 120h, 144h, 168h and 192h after molding, observing the swelling degree of the joint and the like, and taking the serum of the rat and the arthritis at the last time for measuring inflammatory indexes, antioxidant factors and the like.

2. Statistical method

All data were calculated using SAS 9.2 software, the data were expressed as mean. + -. standard deviation (+ -S), the pre-and post-treatment differences or rates of change were compared using Welch rank sum (wilcoxon rank sum) test, and the efficacy was analyzed using logistic regression. p < =0.05 will be considered statistically significant to be tested.

3. Experimental data and conclusions

(1) Swelling degree of joints: the measuring method comprises the following steps: the circumference value of 0.5mm under the right hind-paw treaded joint of each group of rats was measured with a 2-3mm wide paper strip and four stainless steel calipers with a watch. Swelling index definition: the joint circumference, joint swelling index = (joint circumference at the time point measured-initial circumference)/initial circumference, was measured before the experiment and at different times after the induction of inflammation by 2-3mm wide paper strips and four stainless steel strip calipers. The joint swelling degree can be used for evaluating the remission degree of the drug on the gouty arthritis.

The results are shown in table 1:

TABLE 1 Effect on percent increase of ankle circumference (+ -SD) at different time points in rats

The results show that: at each time point, the blank control group and the GA group have significant difference, which indicates that the molding is successful. At 24h, there was a significant difference between the colchicine group and the dose group in the examples of the invention and the GA group, indicating that colchicine and the dose group in the examples of the invention have a therapeutic effect. At 48h, the dose group in the example of the invention has significant difference from the GA group, and has no significant difference from the blank control group. At 72h, the dose group in the examples of the present invention was significantly different from the GA group, and not significantly different from the blank control group and the colchicine group. 96h and later, the dose groups in the examples of the present invention had a tendency to alleviate gouty arthritis.

(2) And (3) index measurement in serum:

the results are shown in table 2:

the results show that:

① α NAG results show that the blank control group has significant difference (p <0.05) with the GA group, which indicates the molding success and can be used as the index of drug evaluation, the colchicine group has significant difference (p <0.01) with the GA group, the colchicine group has no significant difference with the blank control group, which indicates that the colchicine has the treatment effect of GA, the medium dose group has significant difference (p <0.01) with the GA group, and the dose groups in the invention have no significant difference with the blank control group, which indicates that the medium dose has the effect of reducing α NAG level.

② β -gal results show that the blank control group has significant difference (p <0.01) with the GA group, which indicates the molding success and can be used as an index for drug evaluation, the colchicine group has significant difference (p <0.01) with the GA group, the high and medium dose groups have significant difference (p <0.01) compared with the GA group, and the colchicine group has no significant difference with the medium dose group compared with the blank control group, which indicates that both the colchicine group and the medium dose group have β -gal level reduction effect.

③ the results of SOD show that the blank control group has significant difference (p <0.01) with GA group, which indicates the success of molding and can be used as the index of drug evaluation, and the colchicine group, middle dose group and GA group have significant difference (p <0.01), which indicates the significant effect of increasing SOD level of colchicine and middle dose.

④ UA results show that the blank control group has significant difference (p <0.01) with the GA group, which indicates the molding success and can be used as the index of drug evaluation, and the colchicine group, the middle dose group and the GA group have significant difference (p <0.01), and the blank control group has no significant difference, which indicates the significant UA level reducing effect of the colchicine and the middle dose.

(3) Measuring indexes in the joint fluid:

the results are shown in Table 3:

table 3:

① I L-6 shows that the blank control group has significant difference (p <0.05) with the GA group, which indicates the molding success and can be used as the index of drug evaluation, the colchicine group, the high, medium and low dose groups have significant difference (p <0.05) with the GA group, but have no significant difference with the blank control group, which indicates that the colchicine and the Chinese herbal compound with each dose have significant GA treatment effect, wherein the medium dose group has no significant difference with the colchicine group, which indicates that the treatment effects of the two groups are equivalent.

② I L-8 shows that the blank control group has significant difference (p <0.05) with the GA group, which indicates the molding success and can be used as the index of drug evaluation, and the middle dose group has significant difference (p <0.05) with the GA group, but has no significant difference with the blank control group, which indicates the middle dose Chinese herbal compound has significant GA treatment effect.

The results show that:

the traditional Chinese medicine composition has a good effect of treating gouty arthritis, and has the curative effects of improving joint swelling, inflammatory factor change, antioxidant factor change and the like caused by the gouty arthritis.

Claims (1)

1. The traditional Chinese medicine for treating gouty arthritis is characterized by comprising volatile oil and water extract of Chinese herbal medicines, wherein the Chinese herbal medicines comprise the following components in percentage by mass: 10 parts of golden cypress, 10 parts of rhizoma atractylodis, 30 parts of rhizoma smilacis glabrae, 10 parts of liquorice, 15 parts of radix clematidis, 10 parts of radix paeoniae rubra, 15 parts of polygonum cuspidatum, 10 parts of angelica sinensis, 8 parts of Indian iphigenia bulb, 10 parts of radix angelicae pubescentis, 15 parts of codonopsis pilosula and 10;

the preparation method comprises the following steps:

a. mixing cortex Phellodendri, rhizoma Atractylodis, rhizoma Smilacis Glabrae, Glycyrrhrizae radix, radix Clematidis, radix Paeoniae Rubra, rhizoma Polygoni Cuspidati, radix Angelicae sinensis, Pseudobulbus Cremastrae Seu pleiones, radix Angelicae Pubescentis, radix Codonopsis and radix et rhizoma Rhei, pulverizing, placing into a round bottom flask, adding 8 times of purified water, shaking, mixing, and soaking for 30 min;

b. connecting a round-bottom flask with a volatile oil extractor and a reflux condenser tube, adding water from the upper end of the condenser tube until the upper end of the condenser tube is filled with the scale part of the volatile oil detector and overflows into the round-bottom flask, then placing the round-bottom flask in an electric heating jacket to slowly heat the water until the water is boiled and keeps slightly boiling for 6 hours, stopping heating, placing and cooling, starting a piston at the lower end of the volatile oil extractor to slowly discharge the water, and collecting the volatile oil for later use;

c. b, taking the dregs of the decoction after the volatile oil is extracted in the step b, adding 8 times of purified water to decoct for 2 times, each time for 2 hours, filtering, combining the filtrates, and concentrating under reduced pressure to obtain a water extract;

d. mixing the volatile oil and water extract.