CN107467073B - Biological prevention and control agent containing actinomyces is used for the prevention and control and application method of Radix Notoginseng disease - Google Patents

Biological prevention and control agent containing actinomyces is used for the prevention and control and application method of Radix Notoginseng disease Download PDF

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CN107467073B
CN107467073B CN201710740624.0A CN201710740624A CN107467073B CN 107467073 B CN107467073 B CN 107467073B CN 201710740624 A CN201710740624 A CN 201710740624A CN 107467073 B CN107467073 B CN 107467073B
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actinomyces
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CN107467073A (en
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李立梅
陈越渠
侯丽伟
李鑫
朱春玉
陈井生
左彤彤
毛赫
孙伟
孙景花
陈思羽
杨帆
伏颖
陈宁
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JILIN ACADEMY OF FORESTRY
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor

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Abstract

The present invention relates to prevention and control and application method that a kind of biological prevention and control agent containing actinomyces is used for Radix Notoginseng disease, comprising the following steps: prepares 1L culture medium；121 DEG C of sterilizing 30min after the completion of preparation；Actinomyces are inoculated with according to mass ratio 7-10%, cultivation temperature is 30 DEG C, and incubation time is revolving speed 150rpm for 24 hours；According to the rate of 1 DEG C/h, 24 DEG C are cooled the temperature to, cultivates 12h later；Prepare the brown sugar solution of 500g/L, filtration sterilization；The brown sugar solution of the above-mentioned filtration sterilization of 20mL is added into culture medium, temperature is 24 DEG C, cultivates 48h；It collects, the biological prevention and control agent containing actinomyces is made in packaging.It can be effectively controlled the disease of plant using the biological prevention and control agent containing actinomyces prepared by the above method, significantly improve the yield and quality of plant, especially suitable for Radix Notoginseng.

Description

Biological prevention and control agent containing actinomyces is used for the prevention and control and application method of Radix Notoginseng disease
The application is application number 201510607650.7, on 09 23rd, 2015 applying date, " one kind contains puts denomination of invention The divisional application of the preparation method of the biological prevention and control agent of line bacterium ".
Technical field
The present invention relates to the preparation fields of biological prevention and control agent more particularly to a kind of biological prevention and control agent containing actinomyces to be used for Radix Notoginseng The prevention and control and application method of disease.
Background technique
Actinomyces are that one kind is grown in soil, plant surface and inside, and there are also the microbe groups in fresh water or ocean, are People's research is earliest and is widely used in the microorganism in agricultural production.Presently found antibiotics generated bacterium, the overwhelming majority are Actinomyces, and 80% or more antibiotic both be from streptomyces.Streptomycete is primarily present in soil, after spore germination It is initially formed substrate mycelium, is then divided into aerial hyphae, aerial hyphae forms spore chain, re-forms mature spore.Environment item Streptomycete is capable of forming spore when part is not suitable for, and is transferred to dormant state, therefore streptomycete can adapt to a variety of environment.
The actinomyces obtained at present are mainly screened from soil and obtain.On the market, will usually have to pathogen and inhibit to make Different preparations, such as spore pulvis, seed coat agent, Mycelium culture solid dosage forms is made in biological and ecological methods to prevent plant disease, pests, and erosion streptomycete.Above-mentioned dose Type is conducive to commercially producing, transport and saving for biocontrol agent, but generally requires to activate when use, inconvenient for use；If It directly uses, possible control effect is affected.
Radix Notoginseng is araliaceae ginseng plant, is the earliest integration of drinking and medicinal herbs of the distinctive rare traditional Chinese medicine of China and China The root tuber of one of plant, especially Radix Notoginseng has important medical value.Radix Notoginseng has particular/special requirement to the environmental condition of growth, fits It is suitable for weather cool in summer and warm in winter, it is not cold-resistant with extremely hot, half yin of happiness and moist ecological environment.Due to above-mentioned species characteristics, need Plantation, the irrigation intensity of shading for a long time the soil moisture height that arrives greatly, the structure of soil and rhizosphere microorganism group occur acutely Variation.Disinfect pathogen mass propagation causes Severe Reduction, even has no harvest so as to cause prevalence and the outburst of crop disease.At present In order to control the plant disease epidemic of Radix Notoginseng, a large amount of pesticides are used usually in planting process, Yi Yinqi pesticide residue reduces Radix Notoginseng product The problems such as matter.Therefore it provides a kind of biological prevention and control agent suitable for Radix Notoginseng is very urgent and necessary.
Summary of the invention
Technical problem to be solved by the invention is to provide a kind of preparation methods of biological prevention and control agent containing actinomyces, utilize The biological prevention and control agent containing actinomyces of this kind of method preparation can be adapted for Radix Notoginseng, and can be effectively controlled the disease, significant of Radix Notoginseng Improve the yield and quality of Radix Notoginseng.
The technical scheme to solve the above technical problems is that
A kind of preparation method of the biological prevention and control agent containing actinomyces, comprising the following steps:
1) prepare 1L culture medium, the culture medium includes following components: 30g/L soybean powder, 8g/L starch, 3g/L sucrose, 2g/L yeast powder, 2g/L sodium chloride, 1g/L dipotassium hydrogen phosphate, 0.2g/L MgSO4·7H2O, 0.01g/L zinc sulfate, 0.01g/L FeSO4·7H2O, pH 7.2；121 DEG C of sterilizing 30min after the completion of preparation；
2) actinomyces are inoculated with according to mass percent 7%-10%, cultivation temperature is 30 DEG C, and incubation time is revolving speed for 24 hours For 150rpm；
3) according to the rate of 1 DEG C/h, 24 DEG C is cooled the temperature to, cultivates 12h later；
4) the brown sugar solution of 500g/L, filtration sterilization are prepared；
5) the brown sugar solution of the above-mentioned filtration sterilization of 60mL is added into culture medium, temperature is 24 DEG C, cultivates 48h；
6) it collects, the biological prevention and control agent containing actinomyces is made in packaging.
The preparation method of biological prevention and control agent of the present invention containing actinomyces have step is simple, control effect is good, use The beneficial effects such as convenient, quick, specific manifestation are as follows:
1, strict control of the present invention condition of culture, for example, selecting 150rpm that can either guarantee to mix as the revolving speed of culture Even effect is good, is conducive to actinomyces absorbs nutriment, and can not destroy the phage structure of actinomyces itself；With traditional training Feeding actinomyces are in same temperature condition in culture always and compare, and present invention firstly provides first high-temperature culture, gradient drops again The training mode of temperature, the results showed, the control effect of actinomyces can be improved in training mode of the present invention, improves plant The yield and quality of (especially Radix Notoginseng)；
2, the sucrose that the present invention adds in the culture medium of bottom material, later period cool the temperature to 24 DEG C and in this temperature cultures After 12h, and brown sugar is added, the results showed, the viable count angle in the biological prevention and control agent prepared in this way, and can be with Actinomyces vigor and control effect are significantly improved, the yield and quality of plant (especially Radix Notoginseng) is improved；
3, the dosage form of the biological prevention and control agent containing actinomyces of the above method of the present invention preparation is liquid, not according to The preparation method of general biological prevention and control agent is dried, and has the advantage that and 1) simplifies preparation process, reduces preparation Cost；2) the longer holding time is still remained, the biological prevention and control agent prepared by the present invention containing actinomyces is placed in 4 DEG C can be with Stablize and save more than half a year, conjecture is the possible reason is be added more sugar during the bottom material of culture medium and culture culture Substance thus osmotic pressure is higher is conducive to save；3) easy to use, when use, only needs to spray biological prevention and control agent prepared by the present invention Be sprinkled upon at the root soil of plant, be easy to actinomyces play a role, quick result.
Based on the above technical solution, the present invention can also be improved as follows.
Further, the actinomyces are that health shines No. 1, shine agricultural development Co., Ltd purchased from Yang Ling health.
Further, viable count is 5 × 10 in the biological prevention and control agent10To 6 × 1010A/gram.
Contain actinomyces the present invention also provides the preparation method preparation using the above-mentioned biological prevention and control agent containing actinomyces Biological prevention and control agent.
The application method of the above-mentioned biological prevention and control agent containing actinomyces, by taking Radix Notoginseng as an example, comprising the following steps: unwrapping wire will be contained The biological prevention and control agent of bacterium is sprayed at the soil of the root of plant (such as Radix Notoginseng), and every plant (such as Radix Notoginseng) sprinkling contains unwrapping wire The biological prevention and control agent 30-50mL of bacterium.
Biological prevention and control agent of the present invention containing actinomyces is used for the pest and disease damage prevention and control of plant.
Biological prevention and control agent of the present invention containing actinomyces is used for the pest and disease damage prevention and control of Radix Notoginseng.
Biological prevention and control agent of the present invention containing actinomyces is used to improve the yield of Radix Notoginseng, especially raising Radix Notoginseng root tuber Weight.
Specific embodiment
The principles and features of the present invention are described below, and the given examples are served only to explain the present invention, is not intended to limit Determine the scope of the present invention.
Actinomyces used in all embodiments of the invention are purchased from Yang Lingkang according to agricultural development Co., Ltd, entitled health According to No. 1.In previous experience, the Kang Zhao 1 biological control (Niu Rui for being chiefly used in the fruits and vegetables class plant such as tomato, strawberry, eggplant Raw, Fan Jianying, Zhao Xuan, Fu Yali health is grown according to No. 1 Actinomycete preparation to eggplant and the influence Hebei Agricultural Sciences of yield, 2010,14 (5): 41-42).The present invention is used for for the first time in the prevention and control of Radix Notoginseng.
Embodiment 1
1) prepare 1L culture medium, the culture medium includes following components: 30g/L soybean powder, 8g/L starch, 3g/L sucrose, 2g/L yeast powder, 2g/L sodium chloride, 1g/L dipotassium hydrogen phosphate, 0.2g/L MgSO4·7H2O, 0.01g/L zinc sulfate, 0.01g/L FeSO4·7H2O, pH 7.2；121 DEG C of sterilizing 30min after the completion of preparation；
2) according to mass percent 7% be inoculated with actinomyces (i.e. the quality of actinomyces accounts for the 7% of the quality of culture medium, below The meaning of embodiment also same the present embodiment), cultivation temperature is 30 DEG C, and incubation time is revolving speed 150rpm for 24 hours；
3) according to the rate of 1 DEG C/h, 24 DEG C is cooled the temperature to, cultivates 12h later；
4) the brown sugar solution of 500g/L, filtration sterilization are prepared；
5) the brown sugar solution of the above-mentioned filtration sterilization of 60mL is added into culture medium, temperature is 24 DEG C, cultivates 48h；
6) it collects, packaging, the biological prevention and control agent containing actinomyces is made, viable count is 5 × 10 in the biological prevention and control agent10A/ Gram.
Embodiment 2
1) prepare 1L culture medium, the culture medium includes following components: 30g/L soybean powder, 8g/L starch, 3g/L sucrose, 2g/L yeast powder, 2g/L sodium chloride, 1g/L dipotassium hydrogen phosphate, 0.2g/L MgSO4·7H2O, 0.01g/L zinc sulfate, 0.01g/L FeSO4·7H2O, pH 7.2；121 DEG C of sterilizing 30min after the completion of preparation；
2) actinomyces are inoculated with according to mass percent 8%, cultivation temperature is 30 DEG C, and incubation time is for 24 hours that revolving speed is 150rpm；
3) according to the rate of 1 DEG C/h, 24 DEG C is cooled the temperature to, cultivates 12h later；
4) the brown sugar solution of 500g/L, filtration sterilization are prepared；
5) the brown sugar solution of the above-mentioned filtration sterilization of 60mL is added into culture medium, temperature is 24 DEG C, cultivates 48h；
6) it collects, packaging, the biological prevention and control agent containing actinomyces is made, viable count is 5.7 × 10 in the biological prevention and control agent10 A/gram.
Embodiment 3
1) prepare 1L culture medium, the culture medium includes following components: 30g/L soybean powder, 8g/L starch, 3g/L sucrose, 2g/L yeast powder, 2g/L sodium chloride, 1g/L dipotassium hydrogen phosphate, 0.2g/L MgSO4·7H2O, 0.01g/L zinc sulfate, 0.01g/L FeSO4·7H2O, pH 7.2；121 DEG C of sterilizing 30min after the completion of preparation；
2) actinomyces are inoculated with according to mass percent 10%, cultivation temperature is 30 DEG C, and incubation time is for 24 hours that revolving speed is 150rpm；
3) according to the rate of 1 DEG C/h, 24 DEG C is cooled the temperature to, cultivates 12h later；
4) the brown sugar solution of 500g/L, filtration sterilization are prepared；
5) the brown sugar solution of the above-mentioned filtration sterilization of 60mL is added into culture medium, temperature is 24 DEG C, cultivates 48h；
6) it collects, packaging, the biological prevention and control agent containing actinomyces is made, viable count is 6 × 10 in the biological prevention and control agent10A/ Gram.
Embodiment 4
Step 4) in embodiment 2 is changed to " preparing the sucrose solution of 500g/L, filtration sterilization "；By step in embodiment 2 5) it is changed to " sucrose solution for adding the above-mentioned filtration sterilization of 60mL into culture medium be added, temperature is 24 DEG C, cultivates 48h ".Other Step and condition of culture are the same as embodiment 2.
Embodiment 5
Step 5) in embodiment 2 is changed to " the brown sugar solution of the addition above-mentioned filtration sterilization of 30mL into culture medium, temperature to be added Degree is 24 DEG C, cultivates 48h ".Other steps and condition of culture are the same as embodiment 2.
Embodiment 6
Step 5) in embodiment 2 is changed to " the brown sugar solution that the above-mentioned filtration sterilization of 120mL is added into culture medium is added, Temperature is 24 DEG C, cultivates 48h ".Other steps and condition of culture are the same as embodiment 2.
Embodiment 7
Step 1) in embodiment 2 is changed to " to prepare 1L culture medium, the culture medium includes following components: 30g/L soya bean Powder, 8g/L starch, 3g/L brown sugar, 2g/L yeast powder, 2g/L sodium chloride, 1g/L dipotassium hydrogen phosphate, 0.2g/L MgSO4·7H2O、 0.01g/L zinc sulfate, 0.01g/L FeSO4·7H2O, pH 7.2；121 DEG C of sterilizing 30min " after the completion of preparation.Other steps and Condition of culture is the same as embodiment 2.
Embodiment 8
1) prepare 1L culture medium, the culture medium includes following components: 30g/L soybean powder, 8g/L starch, 3g/L sucrose, 2g/L yeast powder, 2g/L sodium chloride, 1g/L dipotassium hydrogen phosphate, 0.2g/L MgSO4·7H2O, 0.01g/L zinc sulfate, 0.01g/L FeSO4·7H2O, pH 7.2；121 DEG C of sterilizing 30min after the completion of preparation；
2) actinomyces are inoculated with according to mass ratio 8%, cultivation temperature is 30 DEG C, incubation time 42h, revolving speed 150rpm；
3) the brown sugar solution of 500g/L, filtration sterilization are prepared；
4) the brown sugar solution of the above-mentioned filtration sterilization of 60mL is added into culture medium, temperature is 24 DEG C, cultivates 48h；
5) it collects, the biological prevention and control agent containing actinomyces is made in packaging.
Embodiment 9
Under the conditions of biological prevention and control agent containing actinomyces prepared by embodiment 2 is placed in 4 DEG C, save 1 year.
For convenience of description, below referred to as to replace, it is specifically shown in Table 1.
Table 1 referred to as and its represents meaning
Referred to as Represent meaning
Product 1 Biological prevention and control agent containing actinomyces prepared by embodiment 1
Product 2 Biological prevention and control agent containing actinomyces prepared by embodiment 2
Product 3 Biological prevention and control agent containing actinomyces prepared by embodiment 3
Comparison 1 Biological prevention and control agent containing actinomyces prepared by embodiment 4
Comparison 2 Biological prevention and control agent containing actinomyces prepared by embodiment 5
Comparison 3 Biological prevention and control agent containing actinomyces prepared by embodiment 6
Comparison 4 Biological prevention and control agent containing actinomyces prepared by embodiment 7
Comparison 5 Biological prevention and control agent containing actinomyces prepared by embodiment 8
Comparison 6 Biological prevention and control agent containing actinomyces prepared by embodiment 9
Comparison 7 Health shines No. 1 pulvis, and viable count is 5 × 1010A/gram
Embodiment 10
The experiment in biennial Radix Notoginseng carries out.
In addition to comparison 7, the said goods directly take 30mL, are sprayed at the soil of the root of Radix Notoginseng respectively, every Radix Notoginseng 30mL is sprayed, every kind of biological prevention and control agent sprays 200；
Comparison 7 is dissolved in 30mL water, the clump count and the clump count in 30mL product 2 of the viable bacteria of the solution after being dissolved in water It is roughly the same, about 1.7 × 1012It is a, 7 spray solution will be compared at the soil of the root of Radix Notoginseng, every Radix Notoginseng sprinkling 30mL sprays 200.
Obtained data are carried out statistical procedures, calculated by the growing state for observing and recording every Radix Notoginseng in 1 year To diseases prevention rate (i.e. using the percentage of the plant number that do not fall ill of certain biological prevention and control agent and total plant), when harvest, counts three The weight of seven root tuber simultaneously calculates separately every Roots of Panax Notoginseng weight after the different biological prevention and control agents of application according to statistical method.Above-mentioned experiment The results are shown in Table 2, and " the every Roots of Panax Notoginseng weight " in table 2 is the result for calculating mean value.
Table 2 is about diseases prevention rate and the experimental data of root weight
It is found in harvest, using product 1, product 2, product 3, the equal appearance uniform of Radix Notoginseng root tuber for comparing 6, in quality Multiply, is better than the quality of the Radix Notoginseng root tuber using comparison 1 to comparison 5, comparison 7.
These results suggest that can be shown using the biological prevention and control agent containing actinomyces prepared by preparation method of the present invention Write the weight for improving the protection effect of Radix Notoginseng and significantly improving the root tuber of Radix Notoginseng.Biological prevention and control agent prepared by the present invention is protected at 4 DEG C Depositing 1 year still has good effect.
Embodiment 11
Fountain height is adjusted to 40mL and 50mL respectively, can also obtain result similar to Example 10.
The foregoing is merely presently preferred embodiments of the present invention, is not intended to limit the invention, it is all in spirit of the invention and Within principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.

Claims (3)

1. a kind of application method of the biological prevention and control agent containing actinomyces, which is characterized in that method includes the following steps: will contain The biological prevention and control agent of actinomyces is sprayed at the soil of the root of plant, and every plant sprays the biological prevention and control agent 30- containing actinomyces 50mL；
Wherein, the biological prevention and control agent containing actinomyces prepares according to the following steps:
1) 1L culture medium is prepared, the culture medium includes following components: 30g/L soybean powder, 8g/L starch, 3g/L sucrose, 2g/L Yeast powder, 2g/L sodium chloride, 1g/L dipotassium hydrogen phosphate, 0.2g/L MgSO4•7H2O, 0.01g/L zinc sulfate, 0.01 g/L FeSO4•7H2O, pH 7.2；121 DEG C of sterilizing 30min after the completion of preparation；
2) actinomyces are inoculated with according to mass percent 7%-10%, cultivation temperature is 30 DEG C, and incubation time is for 24 hours that revolving speed is 150rpm；Actinomyces are that health shines No. 1, shine agricultural development Co., Ltd purchased from Yang Ling health；
3) according to the rate of 1 DEG C/h, 24 DEG C is cooled the temperature to, cultivates 12h later；
4) the brown sugar solution of 500 g/L, filtration sterilization are prepared；
5) the brown sugar solution of the above-mentioned filtration sterilization of 60mL is added into culture medium, temperature is 24 DEG C, cultivates 48 h；
6) it collects, the biological prevention and control agent containing actinomyces is made in packaging.
2. the application method of the biological prevention and control agent containing actinomyces according to claim 1, which is characterized in that the plant is three Seven.
3. the application method of the biological prevention and control agent according to claim 1 containing actinomyces, which is characterized in that the biological and ecological methods to prevent plant disease, pests, and erosion system Viable count is 5 × 10 in agent10To 6 × 1010A/gram.
CN201710740624.0A 2015-09-23 2015-09-23 Biological prevention and control agent containing actinomyces is used for the prevention and control and application method of Radix Notoginseng disease Active CN107467073B (en)

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Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101518264A (en) * 2009-01-22 2009-09-02 中国热带农业科学院热带生物技术研究所 Microbial preparation for preventing and controlling plant silborne fungal diseases, and application thereof

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100334953C (en) * 2003-11-22 2007-09-05 西北农林科技大学 Preparation for biological prevention and cure of hot pepper epidemic disease and method for producing the same
US20150150402A1 (en) * 2012-07-19 2015-06-04 Koninklijke Philips N.V. Device for steaming food
CN104004804B (en) * 2013-02-26 2016-12-28 长沙天赐生物医药科技有限公司 A kind of fermentable preparation method of bleomycin race derivant
CN103194421B (en) * 2013-03-22 2015-05-27 云南省烟草农业科学研究院 Alternaria alternate spore suspension liquid and application thereof

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101518264A (en) * 2009-01-22 2009-09-02 中国热带农业科学院热带生物技术研究所 Microbial preparation for preventing and controlling plant silborne fungal diseases, and application thereof

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Kim,Y.S.等.Screening of Antagonistic Bacteria for Biological control of Ginseng Root Rot.《Korean Journal of Mycology》.2012,第40卷(第1期),参见全文. *
寻路路等.三七根腐病病株和健株根域土壤微生态研究.《西北农业学报》.2013,第31卷(第2期),参见全文. *
牛瑞生等.康照1号放线菌制剂对茄子生长及产量的影响.《河北农业科学》.2010,第14卷(第5期),参见全文. *

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