CN105820974A - Fermenting method of biocontrol actinomycetes and application - Google Patents
Fermenting method of biocontrol actinomycetes and application Download PDFInfo
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Abstract
The invention relates to a fermenting method of biocontrol actinomycetes and application. The method includes the following steps of firstly, preparing a streptomyces virginiae seed bacteria solution and a streptomyces albogriseolus seed bacteria solution, wherein the concentration of bacteria in the streptomyces virginiae seed bacteria solution is 1.2-1.5*10<5-6>/mL, and the concentration of bacteria in the streptomyces albogriseolus seed bacteria solution is 2-5*10<4-5>/mL; secondly, adding a fermentation culture into a 5 L fermentation tank, adding the streptomyces virginiae seed bacteria solution and the streptomyces albogriseolus seed bacteria solution prepared in the first step to be mixed, fermented and cultured to obtain mixed fermentation liquid, wherein the number of bacteria in the mixed fermentation liquid is 2-8*10<6-7>, and the volume ratio of the streptomyces virginiae seed bacteria solution to the streptomyces albogriseolus seed bacteria solution is 1:(2.2-3).By means of the volume ratio of the streptomyces virginiae seed bacteria solution to the streptomyces albogriseolus seed bacteria solution being 1:(2.2-3), the mixing and fermenting of different actinomycetes can be displayed, the reproduction and growth speeds of streptomyces virginiae and streptomyces albogriseolus are both high, and the antagonistic activity of streptomyces virginiae and streptomyces albogriseolus can be well achieved.
Description
Technical field
The present invention relates to fermentation process and the application of a kind of biocontrol actinomycetes, belong to bio-fermented liquid preparation and application neck
Territory.
Background technology
Actinomycetes are to produce the most quasi-microorganism of antibiotic activity material, in hitherto known antibiotic, have and 2/3 are
Produced by actinomycetes.Many antibiotic that actinomycetes produce can produce the strongest suppression killing action to phytopathogen.Its
Middle many antibiotic have been used to prevent and treat the harmful organism on crops, such as jingganmycin, kasugarnycin etc..
In recent years, utilize antagonistic microbe to carry out Biological control and increasingly paid attention to by various countries scientist, the most gradually become
A main trend for control of plant disease research.Actinomycetes are the microbial resources that a class is extremely enriched, the most a lot, especially
Streptomyces can produce Multiple Classes of Antibiotics class material, has wide prospect for control of plant disease.
But actinomycetic fermentation is an extremely complex process, is affected by a lot of environmental conditions, except cultivating
Outside the impact of the carbon-nitrogen ratio of base, trace element, also by incubation time, pH, temperature, be passed through the factors such as air tolerance and affected.Logical
Often in the early stage of actinomycete fermentation, being concrete growth stage, nutrient substance is consumed, antibiotic starts to produce.In fermentation
Phase, antibiotic produces rapidly.To the later stage, antibiotic increasess slowly, it may be possible to owing to thalline is old and feeble, cell starts self-dissolving, fermentation
In liquid, accumulation or other detrimental metabolic of end-product produce impact to thalline, therefore develop and select for different biocontrol microorganisms
The optimization of different fermentation conditions it is critical that.
Fructus Lycopersici esculenti is initially referred to as " wolf Fructus Persicae ".Fruits nutrition enriches, and has flavour.Can eat raw, cook, be processed into kind
Ketchup, juice or whole fruit canning.Fructus Lycopersici esculenti is that one of the most universal fruit and vegetable are cultivated in the whole world.Sweet in the mouth, acid, cool in nature, it is slightly cold.Can heat clearing away
Quench the thirst, yin nourishing, removing heat from blood, return liver, stomach, lung meridian.There is promoting the production of body fluid to quench thirst, invigorating the stomach and promoting digestion, heat-clearing and toxic substances removing, removing heat from blood suppressing the hyperactive liver, blood-supplementing blood-nourishing
With orectic effect.But the morbidity of tomato growth process powdery mildew, anthrax, melasma is serious, the use of a large amount of pesticide,
Fruit pesticide residues are caused to exceed standard.
Summary of the invention
The present invention is to solve that above-mentioned technical problem is to provide fermentation process and the application of a kind of biocontrol actinomycetes.
The technical scheme is that the fermentation process of a kind of biocontrol actinomycetes, particularly
Relate to a kind of compound actinomycetic fermentation process, comprise the following steps:
Step (1) prepares streptomyces virginiae and the seed bacterium solution of white light gray streptomycete, streptomyces virginiae respectively
Seed bacterium solution bacterium amount is 1.2～1.5 × 105-6/mL；White light gray streptomycete 2～5 × 104-5/mL；
Step (2) adds 1-3L fermentation culture in the fermentation tank of 5L, adds step 1) Virginia chain of preparing
Mould species daughter bacteria liquid and white light gray streptomyces strain daughter bacteria liquid carry out mixing fermentation culture and obtain mixed fermentation liquid, mixed fermentation liquid bacterium
Amount is 2-8 × 106-7/mL。
Above-mentioned streptomyces virginiae at China Committee for Culture Collection of Microorganisms's common micro-organisms center number is:
CGMCC No.10042.White light gray streptomycete at China Committee for Culture Collection of Microorganisms's common micro-organisms center number is
CGMCC No.6352。
The invention has the beneficial effects as follows: mixed fermentation of the present invention significantly improves fermentation time, and keeps pathogen simultaneously
There is preferable antagonistic activity.
On the basis of technique scheme, the present invention can also do following improvement.
The fermentation process of the present invention a kind of biocontrol actinomycetes described above, further, step (2) described mixing fermentation culture
During, the volume ratio adding streptomyces virginiae seed bacterium solution and white light gray streptomyces strain daughter bacteria liquid is 1:2.2～3.
Use and above-mentioned enter further to provide the benefit that: owing to using above-mentioned streptomyces virginiae seed bacterium solution and the most shallow
Ash streptomyces strain daughter bacteria liquid volume ratio is the proportionate relationship of 1:2.2～3, can show different actinomycetic mixed fermentation and the two
Flourish speed is all very fast, within can foreshortening to 5 days, and can preferably play the antagonistic activity of mixed fermentation liquid, super
Crossing this proportion, two kinds of bacterium are likely to be due to exist acting on of competing with one another for or suppress and are all difficult under identical condition of culture reach
To the antagonistic activity that it is the highest, i.e. pathogenic fungi is had higher bacteriostatic activity.
The fermentation process of the present invention a kind of biocontrol actinomycetes described above, further, step (1) prepares Virginia respectively
The condition of the seed bacterium solution of streptomycete and white light gray streptomycete is 28 DEG C, 250r/min shaking table cultivation 3 in AAD culture medium respectively
My god, obtain streptomyces virginiae and the seed bacterium solution of white light gray streptomycete respectively.Described shaking table incubation is passed through air
Tolerance is 750～850m3/h。
The fermentation process of the present invention a kind of biocontrol actinomycetes described above, further, described AAD culture medium includes solubility
Starch 4%, analysis for soybean powder 4%, glucose 2%, yeast extract 0.2%, peptone 5%, yeast extract 5%, calcium carbonate 0.2%, phosphoric acid
Potassium dihydrogen 0.2%, sodium chloride 0.2%, magnesium sulfate 0.2%, remainder is water, pH 6.8-7.
The fermentation process of the present invention a kind of biocontrol actinomycetes described above, further, step (2) mixing fermentation culture condition
It is 28 DEG C, 150r-200r/min in fermentation culture by streptomyces virginiae and white light gray streptomycete, shakes training 5 days, it is thus achieved that
Mixed fermentation liquid.
The fermentation process of the present invention a kind of biocontrol actinomycetes described above, further, is passed through air tolerance during shaking training
It is 750～850m3/ h, ventilation is i.e. passed through air, in order to growth of microorganism provides enough oxygen.
The fermentation process of the present invention a kind of biocontrol actinomycetes described above, further, described fermentation culture is solubility
Starch 5%, glycerol 5%, analysis for soybean powder 5%, glucose 5%, yeast extract 5%, peptone 5%, yeast extract 5%, calcium carbonate
0.2%, potassium dihydrogen phosphate 0.2%, sodium chloride 0.2%, magnesium sulfate 0.2%, ferrous sulfate 0.2%, remainder is water, pH 6.9.
Above-mentioned further scheme is used to provide the benefit that: to use above-mentioned specific culture medium prescription can significantly improve mixed
Close the actinomycetes speed of growth and the soonest and can preferably play the active substance antagonistic activity to pathogen in fermentation liquid.
The fermentation process of the present invention a kind of biocontrol actinomycetes described above, further, described streptomyces virginiae Latin
Entitled Streptomyces virginiae；The described white light gray entitled Streptomyces of streptomycete Latin
albogriseolus。
The present invention also provides for fermentation liquid prepared by the fermentation process of a kind of biocontrol actinomycetes in preventing and treating Fructus Lycopersici esculenti powdery mildew, anthrax
Application in phytopathogen caused by sick, melasma and cucumber fusarium axysporum and Fructus Cucumidis sativi phytophthora.
Detailed description of the invention
Being described in conjunction with following principle and feature to the present invention, example is served only for explaining the present invention, not uses
In limiting the scope of the present invention.
Embodiment 1
The fermentation process of a kind of biocontrol actinomycetes of the present invention, comprises the following steps:
Step (1) prepares streptomyces virginiae and the seed bacterium solution of white light gray streptomycete, streptomyces virginiae respectively
Seed bacterium solution bacterium amount is 1.2 × 105, white light gray streptomycete 2 × 104；28 DEG C, 250r/min in AAD culture medium the most respectively
Shaking table is cultivated 3 days, obtains streptomyces virginiae and the seed bacterium solution of white light gray streptomycete respectively, in described shaking table incubation
Being passed through air tolerance is 850m3/h。
AAD culture medium includes soluble starch 4%, analysis for soybean powder 4%, glucose 2%, yeast extract 0.2%, peptone 5%,
Yeast extract 5%, calcium carbonate 0.2%, potassium dihydrogen phosphate 0.2%, sodium chloride 0.2%, magnesium sulfate 0.2%, remainder is water, pH
6.8。
Step (2) adds 1 fermentation culture in the fermentation tank of 5L, adds step 1) streptomyces virginiae prepared
Seed bacterium solution and white light gray streptomyces strain daughter bacteria liquid carry out mixing fermentation culture and obtain mixed fermentation liquid, and mixed fermentation liquid bacterium amount is
2.2×106；Described streptomyces virginiae seed bacterium solution and white light gray streptomyces strain daughter bacteria liquid volume ratio are 1:2.5.Specifically
Ground, mixing fermentation culture condition is 28 DEG C, 150r/min in ADD culture medium, shakes training 5 days, it is thus achieved that mixed fermentation liquid；Shake training process
In to be passed through the tolerance of air be 850m3/h。
Above-mentioned fermentation culture includes soluble starch 5%, glycerol 5%, analysis for soybean powder 5%, glucose 5%, yeast extract
5%, peptone 5%, yeast extract 5%, calcium carbonate 0.2%, potassium dihydrogen phosphate 0.2%, sodium chloride 0.2%, magnesium sulfate 0.2%,
Ferrous sulfate 0.2%, remainder is water, pH 6.9.
Embodiment 2
The fermentation process of a kind of biocontrol actinomycetes of the present invention, comprises the following steps:
Step (1) prepares streptomyces virginiae and the seed bacterium solution of white light gray streptomycete, streptomyces virginiae respectively
Seed bacterium solution bacterium amount is 1.5 × 106/ mL, white light gray streptomycete 5 × 105/mL；The most respectively in AAD culture medium 28 DEG C,
250r/min shaking table is cultivated 3 days, obtains streptomyces virginiae and the seed bacterium solution of white light gray streptomycete respectively, and described shaking table is trained
Being passed through air tolerance during Yanging is 850m3/h。
Above-mentioned concrete AAD culture medium includes soluble starch 4%, analysis for soybean powder 4%, glucose 2%, yeast extract 0.2%, egg
White peptone 5%, yeast extract 5%, calcium carbonate 0.2%, potassium dihydrogen phosphate 0.2%, sodium chloride 0.2%, magnesium sulfate 0.2%, remainder is
Water, pH 7.
Step (2) adds 2L fermentation culture in the fermentation tank of 5L, adds step 1) Virginia strepto-of preparing
Strain daughter bacteria liquid and white light gray streptomyces strain daughter bacteria liquid carry out mixing fermentation culture and obtain mixed fermentation liquid, mixed fermentation liquid bacterium amount
It is 2.8 × 107/mL；Described streptomyces virginiae seed bacterium solution and white light gray streptomyces strain daughter bacteria liquid volume ratio be 1:2.2～
3.Specifically, mixing fermentation culture condition is 28 DEG C, 200r/min in ADD culture medium, shakes training 5 days, it is thus achieved that mixed fermentation liquid；Shake
Being passed through air tolerance during training is 850m3/h。
Above-mentioned fermentation culture includes soluble starch 5%, glycerol 5%, analysis for soybean powder 5%, glucose 5%, yeast extract
5%, peptone 5%, yeast extract 5%, calcium carbonate 0.2%, potassium dihydrogen phosphate 0.2%, sodium chloride 0.2%, magnesium sulfate 0.2%,
Ferrous sulfate 0.2%, remainder is water, pH 6.9.
Embodiment 3
The fermentation process of a kind of biocontrol actinomycetes of the present invention, comprises the following steps:
Step (1) prepares streptomyces virginiae and the seed bacterium solution of white light gray streptomycete, streptomyces virginiae respectively
Seed bacterium solution bacterium amount is 1.2 × 105/ mL, white light gray streptomycete 5 × 104/mL；The most respectively in AAD culture medium 28 DEG C,
250r/min shaking table is cultivated 3 days, obtains streptomyces virginiae and the seed bacterium solution of white light gray streptomycete respectively, and described shaking table is trained
Being passed through air tolerance during Yanging is 850m3/h。
Above-mentioned concrete AAD culture medium includes soluble starch 4%, analysis for soybean powder 4%, glucose 2%, yeast extract 0.2%, egg
White peptone 5%, yeast extract 5%, calcium carbonate 0.2%, potassium dihydrogen phosphate 0.2%, sodium chloride 0.2%, magnesium sulfate 0.2%, remainder is
Water, pH 6.8.
Step (2) adds 3L fermentation culture in the fermentation tank of 5L, adds Virginia strepto-prepared by step (1)
Strain daughter bacteria liquid and white light gray streptomyces strain daughter bacteria liquid carry out mixing fermentation culture and obtain mixed fermentation liquid, mixed fermentation liquid bacterium amount
It is 8 × 106/mL；Described streptomyces virginiae seed bacterium solution and white light gray streptomyces strain daughter bacteria liquid volume ratio are 1:2.2.Tool
Body mixing fermentation culture condition is 28 DEG C, 150r/min in ADD culture medium, shakes training 5 days, it is thus achieved that mixed fermentation liquid；Shake training process
In to be passed through air tolerance be 750m3/h。
Described fermentation culture includes soluble starch 5%, glycerol 5%, analysis for soybean powder 5%, glucose 5%, yeast extract
5%, peptone 5%, yeast extract 5%, calcium carbonate 0.2%, potassium dihydrogen phosphate 0.2%, sodium chloride 0.2%, magnesium sulfate 0.2%,
Ferrous sulfate 0.2%, remainder is water, pH 6.9.
Embodiment 4
The fermentation process of a kind of biocontrol actinomycetes of the present invention, particularly relates to a kind of compound actinomycetic fermentation process, bag
Include following steps:
Step (1) prepares streptomyces virginiae and the seed bacterium solution of white light gray streptomycete, streptomyces virginiae respectively
Seed bacterium solution bacterium amount is 1.2～1.5 × 106/ mL, white light gray streptomycete 2～5 × 105/mL；The most respectively in AAD culture medium
In 28 DEG C, 250r/min shaking table cultivate 3 days, obtain streptomyces virginiae and the seed bacterium solution of white light gray streptomycete, institute respectively
Stating and being passed through air tolerance in shaking table incubation is 750～850m3/h。
Above-mentioned concrete AAD culture medium includes soluble starch 4%, analysis for soybean powder 4%, glucose 2%, yeast extract 0.2%, egg
White peptone 5%, yeast extract 5%, calcium carbonate 0.2%, potassium dihydrogen phosphate 0.2%, sodium chloride 0.2%, magnesium sulfate 0.2%, remainder is
Water, pH 6.8.
Step (2) adds 2.5L fermentation culture in the fermentation tank of 5L, adds step 1) Virginia chain of preparing
Mould species daughter bacteria liquid and white light gray streptomyces strain daughter bacteria liquid carry out mixing fermentation culture and obtain mixed fermentation liquid, mixed fermentation liquid bacterium
Amount is 5.2 × 106/mL；Described streptomyces virginiae seed bacterium solution and white light gray streptomyces strain daughter bacteria liquid volume ratio are 1:3.
Specifically, mixing fermentation culture condition is 28 DEG C, 150r/min in ADD culture medium, shakes training 5 days, it is thus achieved that mixed fermentation liquid；Shake training
During to be passed through air tolerance be 750m3/h。
Described fermentation culture includes soluble starch 5%, glycerol 5%, analysis for soybean powder 5%, glucose 5%, yeast extract
5%, peptone 5%, yeast extract 5%, calcium carbonate 0.2%, potassium dihydrogen phosphate 0.2%, sodium chloride 0.2%, magnesium sulfate 0.2%,
Ferrous sulfate 0.2%, remainder is water, pH 6.9.
Comparative example 1
The fermentation process of a kind of biocontrol actinomycetes of the present invention, particularly relates to a kind of compound actinomycetic fermentation process, bag
Include following steps:
Step (1) prepares streptomyces virginiae and the seed bacterium solution of white light gray streptomycete, streptomyces virginiae respectively
Seed bacterium solution bacterium amount is 1.5 × 106/ mL, white light gray streptomycete 2～5 × 105/mL；The most respectively in Gause I liquid
28 DEG C, 250r/min shaking table cultivate 3 days, obtain streptomyces virginiae and the seed bacterium solution of white light gray streptomycete respectively, described
Being passed through air tolerance in shaking table incubation is 850m3/h。
Step (2) adds fermentation culture in the fermentation tank of 5L, adds step 1) streptomyces virginiae prepared
Seed bacterium solution and white light gray streptomyces strain daughter bacteria liquid carry out mixing fermentation culture and obtain mixed fermentation liquid, and mixed fermentation liquid bacterium amount is
2.3×104/mL；Described streptomyces virginiae seed bacterium solution and white light gray streptomyces strain daughter bacteria liquid volume ratio are that 1:1. is concrete
Ground, mixing fermentation culture condition is 28 DEG C, 150r/min in Gause I liquid, shakes training 20 days, it is thus achieved that mixed fermentation liquid；Shake training
During to be passed through air tolerance be 850m3/h。
Comparative example 2
The fermentation process of a kind of biocontrol actinomycetes of the present invention, particularly relates to a kind of compound actinomycetic fermentation process, bag
Include following steps:
Step (1) prepares streptomyces virginiae seed bacterium solution, and streptomyces virginiae seed bacterium solution bacterium amount is 1.5 ×
106/mL；The most respectively in Gause I liquid 28 DEG C, 250r/min shaking table cultivate 3 days, it is thus achieved that streptomyces virginiae,
Being passed through air tolerance in described shaking table incubation is 850m3/h。
Step (2), to the fermentation cylinder for fermentation culture fluid of 5L, adds step 1) the streptomyces virginiae seed prepared
Bacterium solution carries out fermentation culture and obtains fermentation liquid, and fermentation liquid bacterium amount is 2.3 × 106/mL.Specifically, fermentation culture conditions is Gao Shi
28 DEG C, 150r/min in a number liquid, shake training 16 days, it is thus achieved that fermentation liquid；Being passed through air tolerance during shaking training is 850m3/h。
Comparative example 3
The fermentation process of a kind of biocontrol actinomycetes of the present invention, particularly relates to a kind of compound actinomycetic fermentation process, bag
Include following steps:
Step (1) prepares white light gray streptomyces strain daughter bacteria liquid, and white light gray streptomyces strain daughter bacteria liquid bacterium amount is 1.5 × 106；Tool
Body ground respectively in Gause I liquid 28 DEG C, 250r/min shaking table cultivates 3 days, it is thus achieved that the seed bacterium solution of white light gray streptomycete,
Being passed through air tolerance in described shaking table incubation is 850m3/h.
Step (2) adds fermentation culture in the fermentation tank of 5L, adds step 1) the white light gray streptomycete bacterium prepared
Liquid carries out fermentation culture and obtains fermentation liquid, and fermentation liquid bacterium amount is 2.8 × 106.Specifically, fermentation culture conditions is Gause I liquid
28 DEG C, 150r/min in body, shake training 15 days, it is thus achieved that fermentation liquid；Being passed through air tolerance during shaking training is 850m3/h。
Use embodiment 1 to 4 and comparative example 1 to comparative example 3 fermentation liquid to Fructus Lycopersici esculenti powdery mildew, anthrax, melasma and Huang
Cucurbit wilt bacterium and the bacteriostatic experiment of Fructus Cucumidis sativi phytophthora.
The fermentation liquid that embodiment 1 to 4 and comparative example 1 to comparative example 3 are obtained as raw test sample product, with Fructus Lycopersici esculenti powdery mildew,
Anthrax, melasma and cucumber fusarium axysporum and cucumber phytophthora root rot are for for examination bacterium.By fermentation liquid and PDA culture medium with 1: 9 ratio
It is mixed and made into flat board and is respectively connected to a diameter of 5mm of bacterium cake for examination bacterium in central authorities, and process as comparison using sterilized water, cultivate
3d, uses decussation method to measure antibacterial circle diameter, and each process is repeated 3 times.PDA medium component is Rhizoma Solani tuber osi 200g, Portugal
Grape sugar 20g, agar 15-20g, water 1000ml.
The fermentation liquid of embodiment 1 to 4 and comparative example 1 to comparative example 3 preparation to Fructus Lycopersici esculenti powdery mildew, anthrax, melasma and
The experimental result of cucumber fusarium axysporum and cucumber phytophthora root rot inhibition is shown in Table 1.
Result above shows, the fermentation liquid that mixing fermentation culture of the present invention obtains has good inhibiting effect, with contrast
Example is compared, and can significantly shorten fermentation time, it is expected to make biological pesticide, have higher economic and social benefit.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all spirit in the present invention and
Within principle, any modification, equivalent substitution and improvement etc. made, should be included within the scope of the present invention.
Claims (9)
1. a fermentation process for biocontrol actinomycetes, particularly relates to a kind of compound actinomycetic fermentation process, it is characterised in that
Comprise the following steps:
Step (1) prepares streptomyces virginiae and the seed bacterium solution of white light gray streptomycete, streptomyces virginiae seed respectively
Bacterium solution bacterium amount is 1.2～1.5 × 105-6/mL；White light gray streptomycete bacterium amount is 2～5 × 104-5/mL；
Step (2) adds 1-3L fermentation culture in the fermentation tank of 5L, adds step 1) streptomyces virginiae prepared
Seed bacterium solution and white light gray streptomyces strain daughter bacteria liquid carry out mixing fermentation culture and obtain mixed fermentation liquid, and mixed fermentation liquid bacterium amount is
2～8 × 106-7/mL。
The fermentation process of a kind of biocontrol actinomycetes, it is characterised in that step (2) described mixing is sent out
In ferment incubation, the volume ratio adding streptomyces virginiae seed bacterium solution and white light gray streptomyces strain daughter bacteria liquid is 1:2.2
～3.
The fermentation process of a kind of biocontrol actinomycetes, it is characterised in that step (1) described Fu Jini
The condition of the seed bacterium solution of sub-streptomycete and white light gray streptomycete is 28 DEG C, the training of 250r/min shaking table in AAD culture medium respectively
Support 3 days, obtain streptomyces virginiae and the seed bacterium solution of white light gray streptomycete respectively.
The fermentation process of a kind of biocontrol actinomycetes, it is characterised in that described AAD culture medium includes
Soluble starch 4%, analysis for soybean powder 4%, glucose 2%, yeast extract 0.2%, peptone 5%, yeast extract 5%, calcium carbonate
0.2%, potassium dihydrogen phosphate 0.2%, sodium chloride 0.2%, magnesium sulfate 0.2%, remainder is water, pH 6.8-7.
5. according to the fermentation process of a kind of biocontrol actinomycetes described in any one of Claims 1-4, it is characterised in that step (2) is mixed
Closing fermentation culture conditions is 28 DEG C, 150r-200r/ in fermentation culture by streptomyces virginiae and white light gray streptomycete
Min, shakes training 5 days, it is thus achieved that mixed fermentation liquid.
The fermentation process of a kind of biocontrol actinomycetes, it is characterised in that during shaking training, ventilation is
750～850m3/h。
The fermentation process of a kind of biocontrol actinomycetes, it is characterised in that described fermentation culture is can
Soluble starch 5%, glycerol 5%, analysis for soybean powder 5%, glucose 5%, yeast extract 5%, peptone 5%, yeast extract 5%, calcium carbonate
0.2%, potassium dihydrogen phosphate 0.2%, sodium chloride 0.2%, magnesium sulfate 0.2%, ferrous sulfate 0.2%, remainder is water, pH 6.9.
8. according to the fermentation process of a kind of biocontrol actinomycetes described in any one of Claims 1-4, it is characterised in that described not lucky
Buddhist nun Asia streptomycete Latin entitled Streptomyces virginiae；The described white light gray entitled Streptomyces of streptomycete Latin
albogriseolus。
9. the fermentation liquid that prepared by the fermentation process of biocontrol actinomycetes described in an any one of claim 1 to 8 is white preventing and treating Fructus Lycopersici esculenti
Application in phytopathogen caused by powder disease, anthrax, melasma and cucumber fusarium axysporum and Fructus Cucumidis sativi phytophthora.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109913503A (en) * | 2018-12-27 | 2019-06-21 | 蚌埠学院 | A method of four ene compounds are produced using white light gray streptomycete Ah11601 |
| CN109913384A (en) * | 2018-12-20 | 2019-06-21 | 蚌埠学院 | The preparation method and application of a kind of white light gray streptomycete and its biocontrol agent |
| CN112725228A (en) * | 2020-12-30 | 2021-04-30 | 西南大学 | Virginia streptomycete capable of preventing and treating peach brown rot and application thereof |
| CN115851553A (en) * | 2022-12-30 | 2023-03-28 | 西南大学 | Streptomyces virginiae capable of preventing and treating clubroot and application thereof |
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| CN112725228B (en) * | 2020-12-30 | 2022-10-21 | 西南大学 | Streptomyces virginiae capable of preventing and treating brown rot of peach and application thereof |
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