CN107459100A - A kind of bushing type dirty device only for efficiently removing farmland water body residues of pesticides - Google Patents
A kind of bushing type dirty device only for efficiently removing farmland water body residues of pesticides Download PDFInfo
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- CN107459100A CN107459100A CN201710815962.6A CN201710815962A CN107459100A CN 107459100 A CN107459100 A CN 107459100A CN 201710815962 A CN201710815962 A CN 201710815962A CN 107459100 A CN107459100 A CN 107459100A
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- inner tube
- glass
- pvc transparent
- transparent inner
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/30—Treatment of water, waste water, or sewage by irradiation
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
Abstract
Proposed by the present invention is a kind of bushing type for efficiently removing farmland water body residues of pesticides dirty device only, and its structure includes hollow organic-glass outer tube, PVC transparent inner tube, lucite case, lucite spacer, movable intercept net, neck；Wherein, PVC transparent inner tube is in hollow organic-glass outer tube, and hollow organic-glass outer tube both ends are provided with movable intercept net；Hollow organic-glass outer tube and PVC transparent inner tube are assembled to form net dirty sleeve pipe, and net dirty sleeve pipe is in lucite case, has neck on lucite chamber interior wall, lucite spacer is fixed on neck；The outer tube outer surface of the hollow organic-glass is covered with nano-photocatalyst load layer, and the inner and outer surfaces of PVC transparent inner tube are covered with pesticide degradation bacteria load layer.Advantage：The removal efficiency of farmland water body Pesticide Residues is improved, using conveniently, cost is cheap, can be repeated several times use.
Description
Technical field
The present invention relates to a kind of bushing type dirty device only for efficiently removing farmland water body residues of pesticides, belong to agricultural chemicals face
Source pollution control technology field.
Background technology
Pollution by pesticides has turned into the maximum a kind of organic contamination of China's coverage, and is enriched with continuation and agricultural product
Property；As agricultural chemicals is largely applied for a long time, farmland water-break middle peasant's chemicals contamination getting worse, turn into the important next of agricultural non-point source pollution
One of source, influence aquatile grows and threatens the mankind to be good for after farmland pollution water body enters the aquatic ecosystems such as river, lake
Health, farmland water body Pesticide Residues are effectively removed to ensureing that water environment safety is significant.
In recent years, the research on removing water body Pesticide Residues is concentrated mainly on microbial degradation and photocatalytic degradation two
Individual field；Microbial degradation relies primarily on pesticide degradation bacteria and agricultural chemicals is changed into nonhazardous product by growth metabolism activity（Such as
H2O、CO2）, have the advantages that cost is low, effect is good, non-secondary pollution；Microbial degradation method in actual applications mostly will be micro-
Biology is combined with porous carrier or crosslinking agent is made powdered or granular degradation bacterial agent, is direct plungeed into water body
Row is repaired, and the problems such as recovery is difficult, degradation bacteria is easy to run off be present；Another main direction of studying, photocatalysis degradation of pesticide method
It is to produce photo-generate electron-hole and H using light excitation nano catalyst2O、OH-Effect produces oxidizing species, so as to by agricultural chemicals
It is nonhazardous material Deng organic pollutant degradation.
With the development of photocatalysis technology and the research and development of various new nano material, photocatalysis remove pollution by pesticides have compared with
Big application potential；In current practical application, nano-photocatalyst form is mostly powdered, same in pollution waters restoration
There is the problems such as recovery is difficult, utilization rate is low, latency environment endangers in sample, and nanocatalyst plays a role needs good light source
Covering, and the carrier of existing load nano-photocatalyst is mostly the poor bearer type of the translucency such as porous carbon, it is impossible to make nanometer
Photochemical catalyst plays one's part to the full.
In addition, existing more ripe farmland pollution by pesticides control technology（Such as Artificial Wetland Techniques）Should in actual farmland
With the problems such as middle generally existing floor space is big, cost is high, maintenance difficult, and technology mostly selects a kind of method to agricultural chemicals in itself
Polluted-water is repaired（Such as microorganism remediation method）, due to the restorative procedure of application is single and exist it is a series of it is unstable because
Element, it is difficult to promote the use of a large area；Therefore, a variety of restorative procedures are combined, research and development one kind is efficient, convenient, implements simple, property
Dirty device is extremely urgent only for agricultural chemicals that can be stable.
The content of the invention
The present invention provides a kind of bushing type for efficiently removing farmland water body residues of pesticides dirty device only, and its object is to will be micro-
Biodegradable and photocatalytic degradation use in conjunction, the degradation efficiency of organic agricultural chemicals in the water body of farmland is improved, accelerates residues of pesticides
Remove.
The technical solution of the present invention：A kind of bushing type dirty device only for efficiently removing farmland water body residues of pesticides, its
Structure includes hollow organic-glass outer tube 1, PVC transparent inner tube 2, lucite case 5, lucite spacer 6, movable intercept net
9th, neck 11；Wherein, PVC transparent inner tube 2 is in hollow organic-glass outer tube 1, and the both ends of hollow organic-glass outer tube 1 are provided with
Movable intercept net 9；Hollow organic-glass outer tube 1 and the assembling of PVC transparent inner tube 2 form net dirty sleeve pipe 12, only at dirty sleeve pipe 12
In lucite case 5, there is neck 11 on the inwall of lucite case 5, lucite spacer 6 is fixed on neck 11；In described
Have the outer surface of machine outer glass tube 1 in vain and be covered with nano-photocatalyst load layer 7, the inner and outer surfaces of the PVC transparent inner tube 2 are covered
There is pesticide degradation bacteria load layer 8.
Advantages of the present invention：
（1）Photocatalysis degradation of pesticide is coupled combination by the present apparatus with microbial pesticide degraded, effectively improves farmland water body Pesticides
The removal efficiency of residual.
（2）Bushing type design adds the contact area of polluted-water and net dirty device.
（3）Lucite case, lucite spacer and hollow organic-glass outer tube, PVC transparent flexible pipe have in the present apparatus
There is good translucency, the sleeve pipe assembled can be put into lucite case, and this structure is efficiently separating photochemical catalyst and degraded
While microbial inoculum, light can be passed through again, photocatalytic degradation is effectively ensured and plays a role.
（4）The present apparatus can freely adjust the quantity and specification of built-in sleeve pipe as needed, be applied in farmland water-break irrigation canals and ditches
Convenient, sleeve type structure assembly and disassembly is convenient, and cost is cheap, material easily obtains, and can be repeated several times use.
Brief description of the drawings
Accompanying drawing 1 is present invention practical application schematic diagram in farmland ditch.
Accompanying drawing 2 is the schematic diagram of hollow organic-glass outer tube 1.
Accompanying drawing 3 is the schematic diagram of PVC transparent inner tube 2.
Accompanying drawing 4 is the schematic cross-section that hollow organic-glass outer tube 1 and PVC transparent inner tube 2 are collectively forming net dirty sleeve pipe 12.
1 is hollow organic-glass outer tube in accompanying drawing, and 2 be PVC transparent inner tube（Cluster）, 3 be the partition adjustable collar, and 4 be sleeve pipe
Fixing glue, 5 be lucite case, and 6 be lucite spacer, and 7 be nano-photocatalyst load layer, and 8 be pesticide degradation bacteria load
Layer, 9 be movable intercept net, and 10 be movable intercept net door, and 11 be neck, and 12 be net dirty sleeve pipe.
Embodiment
Only dirty device, its structure include hollow organic-glass to a kind of bushing type for efficiently removing farmland water body residues of pesticides
Outer tube 1, PVC transparent inner tube 2, lucite case 5, lucite spacer 6, movable intercept net 9, neck 11；Wherein, PVC is saturating
Bright inner tube 2 is in hollow organic-glass outer tube 1, and the both ends of hollow organic-glass outer tube 1 are provided with movable intercept net 9；In have in vain
Machine outer glass tube 1 and the assembling of PVC transparent inner tube 2 form net dirty sleeve pipe 12, and net dirty sleeve pipe 12 is in lucite case 5, organic
There is neck 11 on the inwall of glass box 5, lucite spacer 6 is fixed on neck 11；The outer surface of hollow organic-glass outer tube 1
Nano-photocatalyst load layer 7 is covered with, the inner and outer surfaces of the PVC transparent inner tube 2 are covered with pesticide degradation bacteria load layer 8.
There are N number of net dirty sleeve pipe 12, N=5 ~ 20 in the lucite case 5；Each outer surface dress of hollow organic-glass outer tube 1
Flexible spacer ring 3, there are M PVC transparent inner tube 2, M=7~15 in each hollow organic-glass outer tube 1；M PVC transparent inner tube
2 interfix to form tube bundle in PVC transparent by sleeve pipe fixing glue 4 between each other；It is described state sleeve pipe fixing glue 4 divide for ailhead with
Snap-fastener two parts, ailhead can penetrate PVC transparent inner tube 2, negative in PVC transparent inner tube 2 when needing multiple PVC transparent inner tubes 2
After carrying pesticide degradation bacteria load layer 8, PVC transparent inner tube 2 can be penetrated two-by-two with ailhead and be combined with snap-fastener, it is saturating to reach fixing PVC
The purpose of bright interior tube bundle；2 common shape of PVC transparent inner tube in the hollow organic-glass outer tube 1 and hollow organic-glass outer tube 1
Into net dirty sleeve pipe 12.
The surrounding of lucite case 5 is made up of lucite, and both sides are movable intercept net door 10, and intercept net door 10 can
Closure or openness.
Coarse processing is done in the outer surface of hollow organic-glass outer tube 1, and the attachment for being advantageous to improve nano-photocatalyst is strong
Degree；The movable intercept net 9 at the both ends of hollow organic-glass outer tube 1 can be opened, closed, and agricultural chemicals drop is loaded with when needing to be put into
Movable intercept net 9 is opened during the PVC transparent inner tube 2 for solving bacterium, movable intercept net 9 is closed after the completion of being put into and passes through card
Button is fixed, and is easy to tube bundle in PVC 2 being fixed in hollow organic-glass outer tube 1；PVC transparent inner tube 2 loads hollow organic-glass
After outer tube 1, nanometer light that pesticide degradation bacteria that PVC transparent inner tube 2 is loaded is loaded with the outer surface of hollow organic-glass outer tube 1
Catalyst sections are present, without directly contacting, and effectively prevent adverse effect of the nano-photocatalyst to pesticide degradation bacteria.
The PVC transparent inner tube 2 is preferably the flexible transparent round tube of the equal undulate of surfaces externally and internally, and undaform designs energy
Enough dramatically increase the load area of pesticide degradation bacteria.
The inside of lucite case 5 is provided with some necks 11, and lucite spacer 6 is placed on neck 11；The lucite
Dividing plate 6 is movable mode clapboard layer, and the dividing plate laid can be needed according to farmland pollution by pesticides degree and expected repairing effect selection
Lucite case 5 is divided into a number of space, can put on every layer of lucite spacer 6 by quantity, lucite spacer 6
Put net dirty sleeve pipe 12；The lucite case 5 and lucite spacer 6 are all-transparent material, to ensure that it is good that device has
Translucency；The partition adjustable collar 3 outside the net dirty sleeve pipe 12 can play the net dirty sleeve pipe 12 of protection, prevent net dirty sleeve pipe 12
The effect of fragmentation, it is unavoidable to occur sliding now due to being clear glass material when net dirty sleeve pipe 12 is put into lucite case 5
As can effectively prevent net slip of the dirty sleeve pipe 12 on lucite spacer 6 using the partition adjustable collar 3 of anti-skidding material, prolong
The service life of device is grown；The both sides of lucite case 5 are provided with movable intercept net door 10, the movable intercept net door
10 can be opened, close, and convenient net dirty sleeve pipe 12 is placed and taken out in lucite case 5.
The method that the hollow organic-glass outer tube 1 loads nano-photocatalyst load layer 7 is as follows：
1）It is clear that the coarse hollow organic-glass outer tube 1 in outer surface is carried out to ultrasound in dust technology, absolute ethyl alcohol, pure water successively
Wash, supersonic frequency 40KHz, 35 DEG C of temperature, 20 min, dries at room temperature after ultrasonic cleaning every time；
2）The three layers of sealed membrane Apparatus for strapping packages in both ends of hollow organic-glass outer tube 1 after drying, prevent nanometer light to be loaded
Catalyst enters inside hollow organic-glass outer tube 1；The progress nanometer light of machine outer glass tube 1 is had in vain using dip-coating method centering to urge
Agent loads：The hollow organic-glass outer tube 1 of sealing is fixed with crucible tongs or other instruments that can play blessing effect, first
Immerse in nano silicon liquid, hollow organic-glass outer tube 1 is proposed that liquid surface stands 15 by 2~3 min of dipping
Min, above dipping-standing process 2~3 times is repeated, the surface of outer tube 1 is first loaded layer of silicon dioxide film to improve a nanometer light
The intensity of load of catalyst；Then will be loaded with the hollow organic-glass outer tube 1 of nanometer titanium dioxide silicon thin film with crucible tongs or
Other instruments grip, and immerse in nano-photocatalyst load liquid and propose that liquid surface stands 15 min after 5 min, repeat 4
10 min are rinsed to remove the nano-photocatalyst of non-close attachment with pure water after~5 times；Drying at room temperature can obtain after 24 hours
The hollow organic-glass outer tube 1 of nano-photocatalyst load layer 7 must be loaded with；The nano-photocatalyst is preferably Detitanium-ore-type
Nano-TiO2。
The method that the PVC transparent inner tube 2 loads degradation bacteria load layer 8 is as follows：
1）Pesticide degradation bacteria fluid nutrient medium is configured, pesticide degradation bacteria is transferred and expands numerous culture in fluid nutrient medium and obtains agricultural chemicals
Degrade bacterium solution, degradation of pesticide bacterium solution centrifuged into the min of 5 min~15 with the r/min of rotating speed 6000 ~ 8000 and collects to obtain degradation bacteria,
The degradation bacteria and sticking agent are pressed into quality（Bacterium fresh weight）Volume ratio is 1:10（I.e.：10ml sticking agents are added to per 1g degradation bacterias
In）It is well mixed to obtain degradation bacteria-set liquid；The degradation bacteria is to have efficient degradation ability to certain or a variety of organic agricultural chemicals
Bacterial strain, the sticking agent preferably 2% sodium alginate soln；
2）Configure 5%CaCl2Solution, the PVC transparent inner tube 2 is gripped with tweezers or other clamping devices, is submerged initially in 5%
CaCl2Solution immerses in the degradation bacteria-set liquid immediately after impregnating 3 min, 5 min is soaked, then by PVC transparent inner tube 2
It is proposed that liquid surface stands the crosslinking of calcification in 4 hours；Repeat above CaCl2Dipping, degradation bacteria-set liquid dipping, calcification were crosslinked
Journey 4~5 times, 2 drying at room temperature of inner tube is obtained being loaded with to the PVC transparent inner tube 2 of degradation bacteria load layer 8 after 24 hours.
Pesticide degradation bacteria used in the pesticide degradation bacteria load layer 8 is except the specific agriculture obtained after specific domestication culture
Outside medicine degradation bacteria, farmland water body indigenous microorganism also can be selected, improved by coating microorganism fixation layer in PVC transparent inner tube 2
Adhesive ability of the indigenous microorganism in PVC transparent inner tube 2；The carrying method of farmland water body indigenous microorganism：Configure LB agar
Culture medium, the LB agar mediums are containing the g/L of tryptone 10, the g/L of yeast extract 5, the g/L of sodium chloride 10, agar powder 10
G/L, proposed after PVC transparent inner tube 2 is immersed into the min of LB agar mediums 2 after heat sterilization, standing 10 min ensures liquid LB
Culture medium solidifies on the surfaces externally and internally of PVC transparent inner tube 2；Repeat said process 3~4 times, make culture medium in the surfaces externally and internally of inner tube 2
LB agar layers are formed, after device is invested in farmland water-break irrigation canals and ditches, the farmland water body indigenous microorganism in water body will utilize LB
Agar layer accelerates the apposition growth in PVC transparent inner tube 2；Farmland water body indigenous microorganism indigenous microorganism can degrade farmland simultaneously
The Multiple Pesticides remained in water-break, but the increase of required repair time.
In use, being put into outer surface after multiple PVC transparent inner tubes 2 for being loaded with pesticide degradation bacteria are fixed is covered with a nanometer light
Net dirty sleeve pipe 12 is combined into the hollow organic-glass outer tube 1 of catalyst, closes intercept net 9, multiple net dirty sleeve pipes 12 are put into
Lucite case 5, then the lucite case 5 equipped with net dirty sleeve pipe 12 is put into agriculture drainage ditch；By lucite case 5
Intercept net door 10 allows farmland water-break to flow through the net dirty nano surface photochemical catalyst of sleeve pipe 12 in device towards farmland water (flow) direction
Load layer 7 and degradation bacteria load layer 8, reach the purpose of degradation of pesticide and water remediation；The movable intercept net 9 and activity
Formula intercept net door 10 can intercept the impurity such as bulk straw, stone in current, and anti-locking apparatus blocks；When device is in farmland ditch
After middle operation a period of time, net dirty sleeve pipe 12 can be integrally taken out out of lucite case 5, while clear up residual in lucite case 5
Remaining silt；After net dirty sleeve pipe 12 is taken out, hollow organic-glass outer tube 1 therein and PVC transparent inner tube 2 once purged can continue
Nano-photocatalyst and pesticide degradation bacteria are loaded, the net dirty sleeve pipe 12 of loading is repeated after 5 cleaned sand discharge of lucite case and is used；
It should be noted that when rainy season or flood period needing draining a large amount of using irrigation canals and ditches, device must integrally be taken from farmland ditch
Go out, avoid that current block or current are excessive causes tank breaks etc..
In the present invention, hollow organic-glass outer tube 1 and PVC transparent inner tube 2 are collectively forming net dirty sleeve pipe 12, in agriculture drainage ditch
For the interior organic glass box 5 of installation as carrier fixed case, lucite case 5 lays multiple net dirty sleeve pipes 12；When polluted-water flows through
Net dirty sleeve pipe in lucite case 5, the pesticide degradation bacteria and nano-photocatalyst of the net dirty internal load of sleeve pipe 12 play work simultaneously
With；It should be noted that a variety of nano-photocatalysts would generally suppress growth of microorganism, if ensureing photocatalytic degradation and microorganism
Degraded plays a role simultaneously, need to design isolating device by two kinds of load agent isolation, net dirty carrier built in the present apparatus uses inner-outer sleeve
The mode of pipe, outer tube outer surface are loaded with nano-photocatalyst, and inner tube area load has pesticide degradation bacteria, and this structure can effectively divide
From two kinds load agent, make two kinds of biodegrading process organic coupling combinations, effectively improve the removal efficiency of residues of pesticides.
It will be used below specific embodiment to be illustrated the present invention, it should be noted that following examples are not to this
The concrete restriction of invention, function identical place similar to structure of the present invention is within protection scope of the present invention.
Embodiment 1
The bushing type of farmland water body Chlorpyrifos Residue dirty device only is removed based on microorganism-nano-photocatalyst, described receives
Rice photochemical catalyst is anatase type nano TiO2, the microorganism is chlorpyrifos degrading bacteriaSphingomonas sp. Dsp-2。
The selection surface of hollow organic-glass outer tube 1 is through the good lucite material pipe of coarse processing, translucency, external diameter
18cm, internal diameter 16cm, long 50 cm；PVC transparent inner tube 2 is good from translucency, wave PVC material pipe, external diameter 5cm,
Long 50 cm, the size length × width × height of lucite case 5：50 × 60 × 60 cm, the size of lucite spacer 6 length × wide：50×
60 cm；Respective numbers are selected to load the PVC transparent inner tube 2 of chlorpyrifos degrading bacteria load layer 8 during actual use as needed,
Preferably 7 manage, and are interfixed between adjacent two pipe with sleeve pipe fixing glue 4, make the forming sleeves cluster of PVC transparent inner tube 2, and PVC is saturating
Bright interior tube bundle, which is put into, has loaded anatase type nano TiO2The body of hollow organic-glass outer tube 1 in, movable intercept net 9 is closed
Close, prevent interior tube bundle 2 from skidding off；Lucite spacer 6 is inserted into organic glass box 5 makes the casing of lucite case 5 be divided into three layers；
The complete net dirty layering of sleeve pipe 12 of combination is put into lucite case 5, the net dirty set of 9 combinations is laid in lucite case 5
Pipe 12, the partition adjustable ring 3 on the net dirty surface of sleeve pipe 12 can effectively avoid the slip of net dirty sleeve pipe；By movable interception after placing
Net door 10 is closed in the water-break irrigation canals and ditches in the farmland that machine glass box 5 can be put into chronic administration chlopyrifos by fixation, lays direction
Make the intercept net shop front to water body flow direction.
Hollow organic-glass outer tube 1 loads anatase type nano TiO2Method it is as follows：
1）SiO is prepared using sol-gel process2Colloidal sol, in the case where magnetic stirring apparatus rotating speed is 1000 rpm stirring conditions by dilute salt
Aqueous acid at the uniform velocity, is slowly added drop-wise in the ethanol solution of positive silicic acid second vinegar, and absolute ethyl alcohol, hydrochloric acid, positive silicic acid second vinegar rub
You are 2：4：0.05, mixed solution is closed after the backflow of 70 DEG C of constant temperature, 300 rpm are stirred 3 hours, in standing 24 in 33 DEG C of baking ovens
SiO is obtained after hour2Colloidal sol；
2）Detitanium-ore-type TiO is prepared using sol-gel process2Colloidal sol, magnetic stirring apparatus rotating speed are under 1000 rpm stirring conditions
Stirring, the aqueous isopropanol of presoma tetrabutyl titanate is at the uniform velocity added drop-wise in the diluted nitric acid aqueous solution that pH is 2.5, nitric acid and titanium
The mol ratio of sour N-butyl is respectively 76：1.42,75 DEG C of constant temperature stirrings（300 rpm）, gone after flowing back 24 hours with rotary evaporation
Except alcohols, the Detitanium-ore-type TiO of milk shape is finally given2Colloidal sol；The coarse hollow organic-glass outer tube 1 in outer surface is existed successively
It is cleaned by ultrasonic in dust technology, absolute ethyl alcohol, pure water, supersonic frequency 40KHz, 35 DEG C of temperature, every time 20 min, ultrasound is clear
Dried at room temperature after washing；
3）By the both sides of hollow organic-glass outer tube 1 after described dry with three layers of sealed membrane Apparatus for strapping packages, to be loaded receive is prevented
Rice photochemical catalyst enters inside hollow organic-glass outer tube 1；Machine outer glass tube 1 is had in vain using dip-coating method centering and carries out nanometer
Photocatalyst, the hollow organic-glass outer tube 1 of sealing is consolidated with crucible tongs or other instruments that can play blessing effect
It is fixed, it is submerged initially in nano silicon liquid, hollow organic-glass outer tube 1 is proposed that liquid surface is stood by 2~3 min of dipping
15 min, above dipping-standing process 2~3 times is repeated, the surface of outer tube 1 is first loaded layer of silicon dioxide film, then by institute
State and be loaded with the hollow organic-glass outer tube 1 of nano silicon and gripped with crucible tongs or other instruments, immerse rutile titania
Ore deposit type nano-TiO2Load and propose that liquid surface stands 15 min, and 10 are rinsed after repeating 4~5 times with pure water in liquid after 5 min
Min is to remove the anatase type nano TiO of non-close attachment2；Drying at room temperature can obtain after 24 hours and be loaded with Detitanium-ore-type
Nano-TiO2The hollow organic-glass outer tube 1 of load layer 7.
PVC transparent inner tube 2 loads organophosphorus pesticide chlorpyrifos degrading bacteriaSphingomonas sp.Dsp-2 method is such as
Under：
1）To chlorpyrifos degrading bacteriaSphingomonasSp. Dsp-2 is enlarged culture, willSphingomonas sp.
Dsp-2 inoculations are into 4 L LB fluid nutrient mediums；The LB culture mediums contain the g/L of tryptone 10, the g/ of yeast extract 5
L, the g/L of sodium chloride 10；LB medium pHs are 7；Earthquake shaking table is enlarged culture, the rmp of shaking speed 170, cultivation temperature
30 DEG C, detect the growing state of degradation bacteria with light absorption value at the nm of spectrophotometric determination 600, it is to be grown to using after plateau
8000 rpm centrifuge 10 min and collect degradation bacteria；Collected degradation bacteria presses quality（Bacterium fresh weight）Volume ratio is 1:10 and 2% marine alga
Acid sodium solution is well mixed, and degradation bacteria set liquid is made；
2）It is reconfigured at 5%CaCl2Solution, the PVC transparent inner tube 2 is gripped with tweezers or other clamping devices, is submerged initially in
5%CaCl2Solution immerses in the degradation bacteria-set liquid immediately after impregnating 3 min, 5 min is soaked, then by PVC transparent inner tube
2 propose that liquid surface stands the crosslinking of calcification in 4 hours；Repeat above CaCl2Dipping, degradation bacteria-set liquid dipping, calcification were crosslinked
Journey 4~5 times, 2 drying at room temperature of inner tube is obtained being loaded with to the PVC transparent inner tube 2 of degradation bacteria load layer 8 after 24 hours.
Embodiment 2
Farmland water body indigenous microorganism-multiple bushing type associated with nanocatalyst dirty device only, wherein used agricultural chemicals drops
Solution bacterium replaces with farmland water body indigenous microorganism；Nano-photocatalyst selects anatase type nano TiO2。
The selection surface of hollow organic-glass outer tube 1 is through the good lucite material pipe of coarse processing, translucency, external diameter
18cm, internal diameter 16cm, long 50 cm；PVC transparent inner tube 2 is from translucency good, has awave PVC material pipe, outside
Footpath is 5cm, long 50 cm, the size length × width × height of lucite case 5：50 × 60 × 60 cm, the size of lucite spacer 6 length ×
It is wide：50×60 cm；In practical application, respective numbers are selected to load the PVC transparent inner tube 2 of LB agar layers as needed, preferably
Manage for 7, interfixed between adjacent two pipe with sleeve pipe fixing glue 4, PVC transparent inner tube 2 is formed tube bundle in PVC transparent；By PVC
Transparent interior tube bundle, which is put into, has loaded anatase type nano TiO2The body of hollow organic-glass outer tube 1 in, intercept net 9 is closed,
Prevent that tube bundle skids off in PVC transparent；Lucite spacer 6 is inserted into organic glass box 5 makes casing be divided into three layers；It will combine complete
Net dirty sleeve pipe 12 layering be put into lucite case 5, casing can lay 9 net dirty sleeve pipes 12, by intercept net door after placing
Casing can be put into the farmland water-break irrigation canals and ditches of chronic administration agricultural chemicals by 10 closing fixations, and laying direction makes the intercept net shop front to water
Body flow direction.
Hollow organic-glass outer tube 1 loads anatase type nano TiO2Method it is same as Example 1, PVC transparent inner tube 2
Need to load apposition growth of one layer of LB agar medium in order to indigenous microorganism in inner tube 2.Carrying method is as follows：Configuration
LB agar mediums, the LB agar mediums are containing the g/L of tryptone 10, the g/L of yeast extract 5, the g/L of sodium chloride 10, fine jade
The g/L of cosmetics 10, proposed after PVC transparent inner tube 2 is immersed into the min of liquid LB agar mediums 2 after heat sterilization, stand 10 min
Ensure that liquid LB agar mediums solidify on the surfaces externally and internally of inner tube 2；Repeat said process 3~4 times, make culture medium in inner tube 2
Surfaces externally and internally forms LB agar layers, obtains loading the PVC transparent inner tube 2 of LB agar layers, farmland water-break irrigation canals and ditches are invested in device
In after, in the water body indigenous microorganism will utilize LB agar layers accelerate in inner tube 2 apposition growth.
There is broad spectrum activity degradation efficiency compared to specific pesticide degradation bacteria from farmland water body indigenous microorganism, can degrade simultaneously
The Multiple Pesticides of using in farmland, but required repair time compared to directly load efficient degrading bacteria layer device have increased slightly.
Claims (10)
- A kind of 1. bushing type dirty device only for efficiently removing farmland water body residues of pesticides, it is characterized in that outside including hollow organic-glass Pipe, PVC transparent inner tube, lucite case, lucite spacer, movable intercept net, neck；Wherein, at PVC transparent inner tube In hollow organic-glass outer tube, hollow organic-glass outer tube both ends are provided with movable intercept net；Hollow organic-glass outer tube and PVC transparent inner tube is assembled to form net dirty sleeve pipe, and net dirty sleeve pipe is in lucite case, has neck on lucite chamber interior wall, Lucite spacer is fixed on neck；The outer tube outer surface of the hollow organic-glass is covered with nano-photocatalyst load layer, institute The inner and outer surfaces for stating PVC transparent inner tube are covered with pesticide degradation bacteria load layer.
- 2. a kind of bushing type dirty device only for efficiently removing farmland water body residues of pesticides according to claim 1, its feature It is to have N number of net dirty sleeve pipe, N=5 ~ 20 in the lucite case；Each outer tube outer surface of hollow organic-glass is equipped with partition adjustable Ring, there are M PVC transparent inner tube, M=7~15 in each hollow organic-glass outer tube；M PVC transparent inner tube passes through between each other Sleeve pipe fixing glue interfixes to form tube bundle in PVC transparent；The sleeve pipe fixing glue of stating is divided into ailhead and snap-fastener two parts, ailhead PVC transparent inner tube can be penetrated, after PVC transparent inner tube loads pesticide degradation bacteria load layer, PVC transparent is penetrated two-by-two with ailhead Inner tube is simultaneously combined with snap-fastener, reaches the purpose of the transparent interior tube bundle of fixing PVC.
- 3. a kind of bushing type dirty device only for efficiently removing farmland water body residues of pesticides according to claim 1, its feature It is that the lucite case surrounding is made up of lucite, both sides are movable intercept net door, and movable intercept net door can be opened Open or close.
- 4. a kind of bushing type dirty device only for efficiently removing farmland water body residues of pesticides according to claim 1, its feature It is that the outer tube outer surface of the hollow organic-glass does coarse processing, is advantageous to improve the adhesive strength of nano-photocatalyst；It is described The movable intercept net of hollow organic-glass outer tube both sides can be opened, closed, and the PVC of pesticide degradation bacteria is loaded with when needing to be put into Movable intercept net is opened during transparent inner tube, movable intercept net is closed and fixed by snap after the completion of being put into, is easy to Tube bundle in PVC transparent is fixed in hollow organic-glass outer tube；After PVC transparent inner tube loads hollow organic-glass outer tube, PVC The nano-photocatalyst subregion that the pesticide degradation bacteria that transparent inner tube is loaded is loaded with the outer tube outer surface of hollow organic-glass is deposited , without directly contact, effectively prevent adverse effect of the nano-photocatalyst to pesticide degradation bacteria.
- 5. a kind of bushing type dirty device only for efficiently removing farmland water body residues of pesticides according to claim 1, its feature It is the flexible transparent round tube that the PVC transparent inner tube is the equal undulate of surfaces externally and internally.
- 6. a kind of bushing type dirty device only for efficiently removing farmland water body residues of pesticides according to claim 1, its feature It is to be provided with some necks inside the lucite case, lucite spacer is placed on neck；The lucite spacer is work Dynamic formula carrier ring, the dividing plate quantity for needing to lay according to farmland pollution by pesticides degree and expected repairing effect selection, organic glass Lucite case is divided into a number of space by glass dividing plate, and net dirty sleeve pipe can be placed on every layer of lucite spacer； The lucite case and lucite spacer are all-transparent material；The partition adjustable collar of the net dirty jacket exterior plays The net dirty sleeve pipe of protection, the effect for preventing net dirty sleeve pipe fragmentation, when net dirty sleeve pipe is put into lucite case, due to being transparent glass , there is sliding phenomenon unavoidably in glass material, can effectively prevent net dirty sleeve pipe in organic glass using the partition adjustable collar of anti-skidding material Slip on glass dividing plate, extend the service life of device.
- 7. a kind of bushing type dirty device only for efficiently removing farmland water body residues of pesticides according to claim 1, its feature It is that the lucite case both sides are provided with movable intercept net door, the movable intercept net door can be opened, closed, net to facilitate Dirty sleeve pipe is placed and taken out in lucite case.
- 8. a kind of bushing type dirty device only for efficiently removing farmland water body residues of pesticides according to claim 1, its feature Be hollow organic-glass outer tube load nano-photocatalyst load layer method it is as follows：1）The coarse hollow organic-glass outer tube in outer surface is cleaned by ultrasonic in dust technology, absolute ethyl alcohol, pure water successively, Supersonic frequency is 40KHz, and 35 DEG C of temperature, 20 min, dries at room temperature after ultrasonic cleaning every time；2）The three layers of sealed membrane Apparatus for strapping packages in hollow organic-glass outer tube both ends after drying, prevent nanometer light to be loaded from urging Agent enters hollow organic-glass outer tube；Machine outer glass tube is had in vain using dip-coating method centering and carries out nano-photocatalyst Load：Effective crucible tongs outside the hollow organic-glass of sealing or other instruments that can play blessing effect are fixed, are submerged initially in In nano silicon liquid, hollow organic-glass outer tube is proposed that liquid surface stands 15 min by 2~3 min of dipping, is repeated Above dipping-standing process 2~3 times, makes outer pipe surface first load layer of silicon dioxide film to improve nano-photocatalyst Intensity of load；Then effective crucible tongs or other tool holders outside the hollow organic-glass of nanometer titanium dioxide silicon thin film will be loaded with Fixation is held, immerses in nano-photocatalyst load liquid and proposes that liquid surface stands 15 min after 5 min, with pure after repeating 4~5 times Water rinses 10 min to remove the nano-photocatalyst of non-close attachment；Drying at room temperature can obtain after 24 hours and be loaded with nanometer The hollow organic-glass outer tube of photocatalyst layer；The nano-photocatalyst is anatase type nano TiO2。
- 9. a kind of bushing type dirty device only for efficiently removing farmland water body residues of pesticides according to claim 1, its feature Be PVC transparent inner tube load degradation bacteria load layer method it is as follows：1）Pesticide degradation bacteria fluid nutrient medium is configured, pesticide degradation bacteria is transferred and expands numerous culture in fluid nutrient medium and obtains agricultural chemicals Degrade bacterium solution, degradation of pesticide bacterium solution centrifuged into the min of 5 min~15 with the r/min of rotating speed 6000 ~ 8000 and collects to obtain degradation bacteria, It is 1 that the degradation bacteria and sticking agent are pressed into mass volume ratio:10 well mixed obtain degradation bacteria-set liquid；The degradation bacteria is There is the bacterial strain of efficient degradation ability to certain or a variety of organic agricultural chemicals, the sticking agent is 2% sodium alginate soln；2）Configure 5%CaCl2Solution, the PVC transparent inner tube is gripped with tweezers or other clamping devices, is submerged initially in 5% CaCl2Solution immerses in the degradation bacteria-set liquid immediately after impregnating 3 min, soaks 5 min, then puies forward PVC transparent inner tube Go out liquid surface and stand the crosslinking of calcification in 4 hours；Repeat above CaCl2Dipping, degradation bacteria-set liquid dipping, calcification cross-linking process 4 ~5 times, PVC transparent inner tube drying at room temperature is obtained being loaded with to the PVC transparent inner tube of degradation bacteria load layer after 24 hours.
- 10. a kind of bushing type dirty device only for efficiently removing farmland water body residues of pesticides according to claim 1, its feature It is that the pesticide degradation bacteria load layer is farmland water body indigenous microorganism, by coating microorganism fixation layer in PVC transparent inner tube Improve adhesive ability of the farmland water body indigenous microorganism in PVC transparent inner tube；The carrying method of farmland water body indigenous microorganism： LB agar mediums are configured, the LB agar mediums are containing the g/L of tryptone 10, the g/L of yeast extract 5, the g/ of sodium chloride 10 L, the g/L of agar powder 10, after heat sterilization will PVC transparent inner tube immerse the min of LB agar mediums 2 after propose, stand 10 min Ensure that LB liquid medium solidifies on PVC transparent inner tube surfaces externally and internally；Repeat said process 3~4 times, make culture medium in inner tube Surfaces externally and internally forms LB agar layers, after device is invested in farmland water-break irrigation canals and ditches, the farmland water body indigenous microorganism in water body LB agar layers apposition growth in PVC transparent inner tube will be utilized.
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