CN104528954B - A kind of preparation method of degradable composite biological packing - Google Patents
A kind of preparation method of degradable composite biological packing Download PDFInfo
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- CN104528954B CN104528954B CN201510032204.8A CN201510032204A CN104528954B CN 104528954 B CN104528954 B CN 104528954B CN 201510032204 A CN201510032204 A CN 201510032204A CN 104528954 B CN104528954 B CN 104528954B
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- vermiculite
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- 238000012856 packing Methods 0.000 title claims abstract description 19
- 239000002131 composite material Substances 0.000 title claims abstract description 13
- 238000002360 preparation method Methods 0.000 title claims abstract description 10
- CPLXHLVBOLITMK-UHFFFAOYSA-N magnesium oxide Inorganic materials [Mg]=O CPLXHLVBOLITMK-UHFFFAOYSA-N 0.000 claims abstract description 52
- 239000010455 vermiculite Substances 0.000 claims abstract description 46
- 229910052902 vermiculite Inorganic materials 0.000 claims abstract description 46
- 239000000395 magnesium oxide Substances 0.000 claims abstract description 33
- 244000005700 microbiome Species 0.000 claims abstract description 16
- 230000012010 growth Effects 0.000 claims abstract description 15
- AXZKOIWUVFPNLO-UHFFFAOYSA-N magnesium;oxygen(2-) Chemical compound [O-2].[Mg+2] AXZKOIWUVFPNLO-UHFFFAOYSA-N 0.000 claims abstract description 14
- 239000001963 growth medium Substances 0.000 claims abstract description 11
- 239000000843 powder Substances 0.000 claims abstract description 11
- 238000000034 method Methods 0.000 claims abstract description 10
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims description 60
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 claims description 40
- 239000000243 solution Substances 0.000 claims description 30
- 238000003756 stirring Methods 0.000 claims description 25
- 241000894006 Bacteria Species 0.000 claims description 21
- RJIREWQSLPRZFG-UHFFFAOYSA-N 2,5-Diethyl-3-methylpyrazine Chemical compound CCC1=CN=C(CC)C(C)=N1 RJIREWQSLPRZFG-UHFFFAOYSA-N 0.000 claims description 20
- UEGPKNKPLBYCNK-UHFFFAOYSA-L magnesium acetate Chemical compound [Mg+2].CC([O-])=O.CC([O-])=O UEGPKNKPLBYCNK-UHFFFAOYSA-L 0.000 claims description 20
- 239000011654 magnesium acetate Substances 0.000 claims description 20
- 235000011285 magnesium acetate Nutrition 0.000 claims description 20
- 229940069446 magnesium acetate Drugs 0.000 claims description 20
- 229910001629 magnesium chloride Inorganic materials 0.000 claims description 20
- 239000007788 liquid Substances 0.000 claims description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- 239000002245 particle Substances 0.000 claims description 15
- 239000007787 solid Substances 0.000 claims description 12
- 235000019354 vermiculite Nutrition 0.000 claims description 12
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 10
- 239000000725 suspension Substances 0.000 claims description 10
- 241000193755 Bacillus cereus Species 0.000 claims description 8
- 241000006381 Bacillus flexus Species 0.000 claims description 8
- 241000589597 Paracoccus denitrificans Species 0.000 claims description 8
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 8
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 8
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims description 5
- 229960000935 dehydrated alcohol Drugs 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 5
- 238000013467 fragmentation Methods 0.000 claims description 5
- 238000006062 fragmentation reaction Methods 0.000 claims description 5
- 238000010438 heat treatment Methods 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- 230000021332 multicellular organism growth Effects 0.000 claims description 5
- 238000012216 screening Methods 0.000 claims description 5
- 238000002791 soaking Methods 0.000 claims description 5
- 235000010413 sodium alginate Nutrition 0.000 claims description 5
- 239000000661 sodium alginate Substances 0.000 claims description 5
- 229940005550 sodium alginate Drugs 0.000 claims description 5
- CRPCXAMJWCDHFM-UHFFFAOYSA-M sodium;5-oxopyrrolidine-2-carboxylate Chemical compound [Na+].[O-]C(=O)C1CCC(=O)N1 CRPCXAMJWCDHFM-UHFFFAOYSA-M 0.000 claims description 5
- 238000010792 warming Methods 0.000 claims description 5
- 238000005406 washing Methods 0.000 claims description 5
- 239000001888 Peptone Substances 0.000 claims description 2
- 239000004372 Polyvinyl alcohol Substances 0.000 claims description 2
- 235000015278 beef Nutrition 0.000 claims description 2
- 239000008367 deionised water Substances 0.000 claims description 2
- 229910021641 deionized water Inorganic materials 0.000 claims description 2
- 239000002609 medium Substances 0.000 claims description 2
- 239000011259 mixed solution Substances 0.000 claims description 2
- 230000007935 neutral effect Effects 0.000 claims description 2
- 235000015097 nutrients Nutrition 0.000 claims description 2
- 239000002504 physiological saline solution Substances 0.000 claims description 2
- 229920002451 polyvinyl alcohol Polymers 0.000 claims description 2
- 239000013049 sediment Substances 0.000 claims description 2
- 235000013312 flour Nutrition 0.000 abstract 1
- 239000010902 straw Substances 0.000 abstract 1
- 239000000463 material Substances 0.000 description 7
- 239000000945 filler Substances 0.000 description 4
- 238000010008 shearing Methods 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 238000011049 filling Methods 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 239000010865 sewage Substances 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 1
- 239000004593 Epoxy Substances 0.000 description 1
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
- 230000031018 biological processes and functions Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000006213 oxygenation reaction Methods 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000003245 working effect Effects 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/02—Aerobic processes
- C02F3/10—Packings; Fillings; Grids
- C02F3/105—Characterized by the chemical composition
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/04—Enzymes or microbial cells immobilised on or in an organic carrier entrapped within the carrier, e.g. gel or hollow fibres
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
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Abstract
The invention discloses a kind of preparation method of degradable composite biological packing, comprise the steps: 1, by straw powder flour; 2, activated fiber-vermiculite complex body is prepared; 3 prepare magnesium oxide growth media; 4, preparation is adsorbed with the fiber-vermiculite complex body of nano magnesia growth nucleus; 5, the fiber-vermiculite complex body being adsorbed with nano magnesia growth nucleus is soaked; 6, degradable composite biological packing is obtained.The degradable composite biological packing utilizing present method to obtain has better biofilm, interface shear strength, tensile strength, stability, can not affect the normal growth of microorganism.
Description
Technical field
The present invention relates to a kind of preparation method of degradable composite biological packing, belong to field of environment protection.
Background technology
The sewage water treatment method of current employing has chemical method, Physical, biological process.Utilizing microorganism polluted-water to be processed to the focus direction of the research being current water treatment field, is also a kind of green high-efficient, the process means of non-secondary pollution.Wherein biomembrance process is degraded to the pollutent in sewage by the micropopulation on microbial film in filling surface biofilm.
The biologic packing material used at present all in various degree there is defect.Biomembrance process floor space is little, efficiency is high.But due to the fluctuation of drainage flow and water quality, the microorganism added is easy to run off, or is preyed on by protozoon, be difficult to keep the stable removal effect continued.Fixed packing specific area is little, biofilm biomass is few, and smooth surface, microbial film easily comes off.Thus, cause these fillers in use often can run into the shortcomings such as blocking, conglomeration, gas distribution water distribution is uneven, oxygenation capacity is poor.Shape ceramics filling, in the research of process source water micro-pollution, although carrier surface forming biofilm property is good, flow resistance is large, easily blocks, intensity difference, easily broken, not water-fastly washes away, and limits its application.The performance of performance to immobilized microorganism function of filler material plays vital effect.The feature of present immobilized microorganism material ubiquity poor stability, microorganism colonization poor performance, mechanical property difference.
Summary of the invention
For above-mentioned prior art Problems existing, the invention provides a kind of preparation method of degradable composite biological packing, the method comprises the steps:
(1), get stalk, through fragmentation, supper micron mill is pulverized and after ultrafine mill refinement, is obtained granularity and be about 200 object powder particles, for subsequent use;
(2), take the vermiculite of 100 mass parts, screening after pulverizer is pulverized, obtain particle diameter and be less than 200 object vermiculite powers, be placed in retort furnace, with the temperature rise rate of 10 DEG C/min, room temperature is naturally cooled to after being warming up to 300 DEG C, the powder particle obtained with 300 mass parts steps (1) mixes, then soak 5h by the 2-Pyrrolidone-5-carboxylic acid sodium of 200 mass parts and form solidliquid mixture, add dehydrated alcohol more wherein to stir evenly, leave standstill centrifugal treating after 2 hours afterwards, take out solid matter, with absolute ethanol washing 3 ~ 5 times, then solid matter is dried under the condition of 80 DEG C, obtain activated fiber-vermiculite complex body,
(3), by magnesium acetate join in ethylene glycol, stir under temperature is 30 DEG C ~ 40 DEG C and stirring velocity is the condition of 500r/min and obtain magnesium acetate/ethylene glycol solution, the volumetric molar concentration of described magnesium acetate/ethylene glycol is 0.3 ~ 0.5M; Then in magnesium acetate/ethylene glycol solution, add the nonionogenic tenside accounting for mixeding liquid volume 1 ~ 5%, continue stirring and obtain magnesium oxide growth media in 1.5-3 hour;
(4), activated fiber-vermiculite complex body that 100 mass parts steps (2) obtain is immersed in the magnesium oxide growth media that 200 mass parts steps (3) obtain, at room temperature stir, then dry 1min ~ 10min at temperature is 100 DEG C ~ 200 DEG C, obtains the fiber-vermiculite complex body being adsorbed with nano magnesia growth nucleus;
(5), in magnesium chloride solution, 2 are added, 5-diethyl-3-methylpyrazine, obtain 2,5-diethyl-3-methylpyrazine/magnesium chloride solution, by 2,5-diethyl-3-methylpyrazine/magnesium chloride solution is heated to 30 DEG C ~ 60 DEG C, after the heating that the fiber-vermiculite complex body being adsorbed with nano magnesia growth nucleus step (4) obtained is immersed in 2, in 5-diethyl-3-methylpyrazine/magnesium chloride solution, soaking 2h ~ 20h makes bitter earth nano nucleus in fiber-vermiculite complex body growth, then takes out at room temperature dry;
(6), the nano magnesia that is adsorbed with that mixed bacteria liquid uniform application obtains in step (5) is grown in the fiber-vermiculite complex surfaces of nucleus, smear the sodium alginate soln that mass percent is 0.5% again, place after about 2-4 hour by clean water, at 40 DEG C, drying 20 hours, obtains degradable composite biological packing.
In step (6), the preparation method of mixed bacteria liquid is as follows:
Substratum is utilized to filter out Paracoccus denitrificans, Bacillus flexus and saccharomyces cerevisiae and bacillus cereus from water body or mud;
Paracoccus denitrificans, Bacillus flexus, saccharomyces cerevisiae and bacillus cereus are accessed beef extract-peptone liquid nutrient medium respectively, after 36 DEG C of cultivation 24h, centrifugal 10min under the rotating speed of 5000rpm, by resuspended 3 times of the solid sediment physiological saline that obtains, obtains four kinds of bacteria suspensions;
By the bacteria suspension of the bacteria suspension of the bacteria suspension of Paracoccus denitrificans, Bacillus flexus, the bacteria suspension of saccharomyces cerevisiae and bacillus cereus by 1: 2: 1: 2 volume ratio be mixed to get microorganism species, access in substratum obtain plastc ring to connect bacterium amount 9%;
The plastc ring of the saccharomyces cerevisiae obtained, Paracoccus denitrificans, Bacillus flexus and bacillus cereus is put into container, and the pH value being diluted to mixed solution with deionized water, in neutral, add polyvinyl alcohol and forms mixed bacteria liquid.。
The invention has the advantages that:
(1) vermiculite of the present invention and stalk fibre silver carry out compound tense, add the specific surface area of whole matrix material, make it have excellent microorganism colonization performance; Nano magnesia, in even, the intensive growth of fiber-vermiculite complex surfaces, improves the degree of roughness on surface, further increases the specific surface area of filler and the bond area of microorganism, the carrier surface forming biofilm property of biologic packing material is improved further;
(2) interface shear strength of the modified reinforced epoxy more before modified of magnesia fibers of the present invention improves 30% ~ 51%, and tensile strength enhances 20-32%, improves the work-ing life of filler;
(3) use the low raw-material cost such as vermiculite, stalk in the present invention, rich reserves, and production technique is simple, with microorganism compound tense, the normal growth of microorganism can not be affected, also substantially can not bring microorganism and poison reaction, avoid simultaneously and secondary pollution is caused to water body.
Embodiment
Below in conjunction with embodiment, the invention will be further described.
embodiment 1
(1), get stalk, through fragmentation, supper micron mill is pulverized and after ultrafine mill refinement, is obtained granularity and be about 200 object powder particles, for subsequent use;
(2), take the vermiculite of 100 mass parts, screening after pulverizer is pulverized, obtain particle diameter and be less than 200 object vermiculite powers, be placed in retort furnace, with the temperature rise rate of 10 DEG C/min, room temperature is naturally cooled to after being warming up to 300 DEG C, the powder particle obtained with 300 mass parts steps (1) mixes, then soak 5h by the 2-Pyrrolidone-5-carboxylic acid sodium of 200 mass parts and form solidliquid mixture, add dehydrated alcohol more wherein to stir evenly, leave standstill centrifugal treating after 2 hours afterwards, take out solid matter, with absolute ethanol washing 3 ~ 5 times, then solid matter is dried under the condition of 80 DEG C, obtain activated fiber-vermiculite complex body,
(3), by magnesium acetate join in ethylene glycol, stir under temperature is 30 DEG C ~ 40 DEG C and stirring velocity is the condition of 500r/min and obtain magnesium acetate/ethylene glycol solution, the volumetric molar concentration of described magnesium acetate/ethylene glycol is 0.3M; Then in magnesium acetate/ethylene glycol solution, add the nonionogenic tenside accounting for mixeding liquid volume 1%, continue stirring and obtain magnesium oxide growth media in 1.5-3 hour;
(4), activated fiber-vermiculite complex body that 100 mass parts steps (2) obtain is immersed in the magnesium oxide growth media that 200 mass parts steps (3) obtain, at room temperature stir, then dry 1min ~ 10min at temperature is 100 DEG C, obtains the fiber-vermiculite complex body being adsorbed with nano magnesia growth nucleus;
(5), in magnesium chloride solution, 2 are added, 5-diethyl-3-methylpyrazine, obtain 2,5-diethyl-3-methylpyrazine/magnesium chloride solution, by 2,5-diethyl-3-methylpyrazine/magnesium chloride solution is heated to 30 DEG C ~ 60 DEG C, after the heating that the fiber-vermiculite complex body being adsorbed with nano magnesia growth nucleus step (4) obtained is immersed in 2, in 5-diethyl-3-methylpyrazine/magnesium chloride solution, soaking 2h ~ 20h makes bitter earth nano nucleus in fiber-vermiculite complex body growth, then takes out at room temperature dry;
(6), the nano magnesia that is adsorbed with that mixed bacteria liquid uniform application obtains in step (5) is grown in the fiber-vermiculite complex surfaces of nucleus, smear the sodium alginate soln that mass percent is 0.5% again, place after about 2-4 hour by clean water, at 40 DEG C, drying 20 hours, obtains degradable composite biological packing.
The magnesium oxide modified rear interface shearing resistance of this biologic packing material improves 30%, and tensile strength enhances 25%, and microorganism colonization amount improves 32%.
embodiment 2
(1), get stalk, through fragmentation, supper micron mill is pulverized and after ultrafine mill refinement, is obtained granularity and be about 200 object powder particles, for subsequent use;
(2), take the vermiculite of 100 mass parts, screening after pulverizer is pulverized, obtain particle diameter and be less than 200 object vermiculite powers, be placed in retort furnace, with the temperature rise rate of 10 DEG C/min, room temperature is naturally cooled to after being warming up to 300 DEG C, the powder particle obtained with 300 mass parts steps (1) mixes, then soak 5h by the 2-Pyrrolidone-5-carboxylic acid sodium of 200 mass parts and form solidliquid mixture, add dehydrated alcohol more wherein to stir evenly, leave standstill centrifugal treating after 2 hours afterwards, take out solid matter, with absolute ethanol washing 3 ~ 5 times, then solid matter is dried under the condition of 80 DEG C, obtain activated fiber-vermiculite complex body,
(3), by magnesium acetate join in ethylene glycol, stir under temperature is 30 DEG C ~ 40 DEG C and stirring velocity is the condition of 500r/min and obtain magnesium acetate/ethylene glycol solution, the volumetric molar concentration of described magnesium acetate/ethylene glycol is 0.4M; Then in magnesium acetate/ethylene glycol solution, add the nonionogenic tenside accounting for mixeding liquid volume 3%, continue stirring and obtain magnesium oxide growth media in 1.5-3 hour;
(4), activated fiber-vermiculite complex body that 100 mass parts steps (2) obtain is immersed in the magnesium oxide growth media that 200 mass parts steps (3) obtain, at room temperature stir, then dry 1min ~ 10min at temperature is 150 DEG C, obtains the fiber-vermiculite complex body being adsorbed with nano magnesia growth nucleus;
(5), in magnesium chloride solution, 2 are added, 5-diethyl-3-methylpyrazine, obtain 2,5-diethyl-3-methylpyrazine/magnesium chloride solution, by 2,5-diethyl-3-methylpyrazine/magnesium chloride solution is heated to 30 DEG C ~ 60 DEG C, after the heating that the fiber-vermiculite complex body being adsorbed with nano magnesia growth nucleus step (4) obtained is immersed in 2, in 5-diethyl-3-methylpyrazine/magnesium chloride solution, soaking 2h ~ 20h makes bitter earth nano nucleus in fiber-vermiculite complex body growth, then takes out at room temperature dry;
(6), the nano magnesia that is adsorbed with that mixed bacteria liquid uniform application obtains in step (5) is grown in the fiber-vermiculite complex surfaces of nucleus, smear the sodium alginate soln that mass percent is 0.5% again, place after about 2-4 hour by clean water, at 40 DEG C, drying 20 hours, obtains degradable composite biological packing.
The magnesium oxide modified rear interface shearing resistance of this biologic packing material improves 42%, and tensile strength enhances 32%, and microorganism colonization amount improves 41%.
embodiment 3
(1), get stalk, through fragmentation, supper micron mill is pulverized and after ultrafine mill refinement, is obtained granularity and be about 200 object powder particles, for subsequent use;
(2), take the vermiculite of 100 mass parts, screening after pulverizer is pulverized, obtain particle diameter and be less than 200 object vermiculite powers, be placed in retort furnace, with the temperature rise rate of 10 DEG C/min, room temperature is naturally cooled to after being warming up to 300 DEG C, the powder particle obtained with 300 mass parts steps (1) mixes, then soak 5h by the 2-Pyrrolidone-5-carboxylic acid sodium of 200 mass parts and form solidliquid mixture, add dehydrated alcohol more wherein to stir evenly, leave standstill centrifugal treating after 2 hours afterwards, take out solid matter, with absolute ethanol washing 3 ~ 5 times, then solid matter is dried under the condition of 80 DEG C, obtain activated fiber-vermiculite complex body,
(3), by magnesium acetate join in ethylene glycol, stir under temperature is 30 DEG C ~ 40 DEG C and stirring velocity is the condition of 500r/min and obtain magnesium acetate/ethylene glycol solution, the volumetric molar concentration of described magnesium acetate/ethylene glycol is 0.5M; Then in magnesium acetate/ethylene glycol solution, add the nonionogenic tenside accounting for mixeding liquid volume 5%, continue stirring and obtain magnesium oxide growth media in 1.5-3 hour;
(4), activated fiber-vermiculite complex body that 100 mass parts steps (2) obtain is immersed in the magnesium oxide growth media that 200 mass parts steps (3) obtain, at room temperature stir, then dry 1min ~ 10min at temperature is 200 DEG C, obtains the fiber-vermiculite complex body being adsorbed with nano magnesia growth nucleus;
(5), in magnesium chloride solution, 2 are added, 5-diethyl-3-methylpyrazine, obtain 2,5-diethyl-3-methylpyrazine/magnesium chloride solution, by 2,5-diethyl-3-methylpyrazine/magnesium chloride solution is heated to 30 DEG C ~ 60 DEG C, after the heating that the fiber-vermiculite complex body being adsorbed with nano magnesia growth nucleus step (4) obtained is immersed in 2, in 5-diethyl-3-methylpyrazine/magnesium chloride solution, soaking 2h ~ 20h makes bitter earth nano nucleus in fiber-vermiculite complex body growth, then takes out at room temperature dry;
(6), the nano magnesia that is adsorbed with that mixed bacteria liquid uniform application obtains in step (5) is grown in the fiber-vermiculite complex surfaces of nucleus, smear the sodium alginate soln that mass percent is 0.5% again, place after about 2-4 hour by clean water, at 40 DEG C, drying 20 hours, obtains degradable composite biological packing.
The magnesium oxide modified rear interface shearing resistance of this biologic packing material improves 51%, and tensile strength enhances 42%, and microorganism colonization amount improves 49%.
Claims (2)
1. a preparation method for degradable composite biological packing, is characterized in that the method comprises the steps:
(1), get stalk, through fragmentation, supper micron mill is pulverized and after ultrafine mill refinement, is obtained granularity and be about 200 object powder particles, for subsequent use;
(2), take the vermiculite of 100 mass parts, screening after pulverizer is pulverized, obtain particle diameter and be less than 200 object vermiculite powers, be placed in retort furnace, with the temperature rise rate of 10 DEG C/min, room temperature is naturally cooled to after being warming up to 300 DEG C, the powder particle obtained with 300 mass parts steps (1) mixes, then soak 5h by the 2-Pyrrolidone-5-carboxylic acid sodium of 200 mass parts and form solidliquid mixture, add dehydrated alcohol more wherein to stir evenly, leave standstill centrifugal treating after 2 hours afterwards, take out solid matter, with absolute ethanol washing 3 ~ 5 times, then solid matter is dried under the condition of 80 DEG C, obtain activated fiber-vermiculite complex body,
(3), by magnesium acetate join in ethylene glycol, stir under temperature is 30 DEG C ~ 40 DEG C and stirring velocity is the condition of 500r/min and obtain magnesium acetate/ethylene glycol solution, the volumetric molar concentration of described magnesium acetate/ethylene glycol is 0.3 ~ 0.5M; Then in magnesium acetate/ethylene glycol solution, add the nonionogenic tenside accounting for mixeding liquid volume 1 ~ 5%, continue stirring and obtain magnesium oxide growth media in 1.5-3 hour;
(4), activated fiber-vermiculite complex body that 100 mass parts steps (2) obtain is immersed in the magnesium oxide growth media that 200 mass parts steps (3) obtain, at room temperature stir, then dry 1min ~ 10min at temperature is 100 DEG C ~ 200 DEG C, obtains the fiber-vermiculite complex body being adsorbed with nano magnesia growth nucleus;
(5), in magnesium chloride solution, 2 are added, 5-diethyl-3-methylpyrazine, obtain 2,5-diethyl-3-methylpyrazine/magnesium chloride solution, by 2,5-diethyl-3-methylpyrazine/magnesium chloride solution is heated to 30 DEG C ~ 60 DEG C, fiber-vermiculite the complex body being adsorbed with nano magnesia growth nucleus step (4) obtained is immersed in 2 after heating, in 5-diethyl-3-methylpyrazine/magnesium chloride solution, soaking 2h ~ 20h makes bitter earth nano nucleus in fiber-vermiculite complex body growth, then takes out at room temperature dry;
(6), the nano magnesia that is adsorbed with that mixed bacteria liquid uniform application obtains in step (5) is grown in the fiber-vermiculite complex surfaces of nucleus, smear the sodium alginate soln that mass percent is 0.5% again, place after 2-4 hour by clean water, at 40 DEG C, drying 20 hours, obtains degradable composite biological packing.
2. preparation method as claimed in claim 1, is characterized in that, in step (6), the preparation method of mixed bacteria liquid is as follows:
Substratum is utilized to filter out Paracoccus denitrificans, Bacillus flexus, saccharomyces cerevisiae and bacillus cereus from water body or mud;
Paracoccus denitrificans, Bacillus flexus, saccharomyces cerevisiae and bacillus cereus are accessed beef extract-peptone liquid nutrient medium respectively, after 36 DEG C of cultivation 24h, centrifugal 10min under the rotating speed of 5000rpm, by resuspended 3 times of the solid sediment physiological saline that obtains, obtains four kinds of bacteria suspensions;
By the bacteria suspension of the bacteria suspension of the bacteria suspension of Paracoccus denitrificans, Bacillus flexus, the bacteria suspension of saccharomyces cerevisiae and bacillus cereus by 1: 2: 1: 2 volume ratio be mixed to get microorganism species, access in substratum obtain plastc ring to connect bacterium amount 9%;
The plastc ring of the saccharomyces cerevisiae obtained, Paracoccus denitrificans, Bacillus flexus and bacillus cereus is put into container, and the pH value being diluted to mixed solution with deionized water, in neutral, add polyvinyl alcohol and forms mixed bacteria liquid.
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| CN101905149B (en) * | 2010-07-01 | 2011-11-23 | 傅桂云 | Composite material for absorbing harmful substances in water |
| US20130277302A1 (en) * | 2012-04-24 | 2013-10-24 | Klaus Doelle | Water treatment system |
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| CN101905149B (en) * | 2010-07-01 | 2011-11-23 | 傅桂云 | Composite material for absorbing harmful substances in water |
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