CN104743680B - A kind of preparation method of modified pearl rock biologic packing material - Google Patents

A kind of preparation method of modified pearl rock biologic packing material Download PDF

Info

Publication number
CN104743680B
CN104743680B CN201510155211.7A CN201510155211A CN104743680B CN 104743680 B CN104743680 B CN 104743680B CN 201510155211 A CN201510155211 A CN 201510155211A CN 104743680 B CN104743680 B CN 104743680B
Authority
CN
China
Prior art keywords
preparation
dried
water
pearl rock
zsm
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201510155211.7A
Other languages
Chinese (zh)
Other versions
CN104743680A (en
Inventor
李文新
狄君绿
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Li Jianzhen
Original Assignee
Jinhua Zhichan Wuyuan Information Technology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jinhua Zhichan Wuyuan Information Technology Co Ltd filed Critical Jinhua Zhichan Wuyuan Information Technology Co Ltd
Priority to CN201510155211.7A priority Critical patent/CN104743680B/en
Publication of CN104743680A publication Critical patent/CN104743680A/en
Application granted granted Critical
Publication of CN104743680B publication Critical patent/CN104743680B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/34Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/02Aerobic processes
    • C02F3/10Packings; Fillings; Grids
    • C02F3/105Characterized by the chemical composition
    • C02F3/107Inorganic materials, e.g. sand, silicates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • C12N1/145Fungal isolates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • C12N1/16Yeasts; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • C12N1/16Yeasts; Culture media therefor
    • C12N1/18Baker's yeast; Brewer's yeast
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • C12N1/16Yeasts; Culture media therefor
    • C12N1/18Baker's yeast; Brewer's yeast
    • C12N1/185Saccharomyces isolates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • C12N1/205Bacterial isolates
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00Nature of the contaminant
    • C02F2101/30Organic compounds
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/01Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07Bacillus
    • C12R2001/085Bacillus cereus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/01Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07Bacillus
    • C12R2001/10Bacillus licheniformis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/645Fungi ; Processes using fungi
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/645Fungi ; Processes using fungi
    • C12R2001/85Saccharomyces
    • C12R2001/865Saccharomyces cerevisiae
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W10/00Technologies for wastewater treatment
    • Y02W10/10Biological treatment of water, waste water, or sewage

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Botany (AREA)
  • Hydrology & Water Resources (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Environmental & Geological Engineering (AREA)
  • Water Supply & Treatment (AREA)
  • Inorganic Chemistry (AREA)
  • Materials Engineering (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Biological Treatment Of Waste Water (AREA)

Abstract

The invention discloses the preparation method of a kind of modified pearl rock biologic packing material, comprise the steps: 1, configure modification liquid;2, the preparation of modified pearl rock;3, the preparation of Ti/Zn ZSM 5;4, filling carrier is prepared;5, by mixed bacteria liquid uniform application on the filling carrier surface that step 4 obtains, repaste and smear the sodium alginate soln that mass percent is 0.5%, by clean water after placing about 24 hours, be dried 20 hours at 40 DEG C, obtain modification biological filler.The modification biological filler utilizing this method to obtain has more preferable biofilm, can decompose long chain alkane and polycyclic aromatic hydrocarbon by active adsorption, does not interferes with the normal growth of microorganism.

Description

A kind of preparation method of modified pearl rock biologic packing material
Technical field
The present invention relates to the preparation method of a kind of modified pearl rock biologic packing material, belong to field of environment protection.
Background technology
Along with quickly increasing and industrialized propelling of the size of population, the pollution level of water body is day by day serious, and occurs Organic difficult decomposed substance and tradition ammonia nitrogen, the novel pollution type of the compound pollution of COD, traditional wastewater processing method is to this type of The treatment effect of sewage is the best.Utilizing microorganism to process polluted-water is a research of current water treatment field Focus direction, be also a kind of green high-efficient, the process means of non-secondary pollution.Wherein biomembrance process is in filling surface biofilm By the micropopulation on biomembrane, the pollutant in sewage are degraded.
The biologic packing material used at present the most in various degree there is defect.Present immobilized microorganism material is universal Existence and stability is poor, the feature of microorganism colonization poor performance, mechanical property difference.Due to drainage flow and the fluctuation of water quality, The microorganism added is easy to run off, or is preyed on by protozoacide, it is difficult to keep stablizing lasting removal effect.Fixed fill out Specific surface area is little, biofilm biomass is few for material, and smooth surface, and biomembrane is easy to fall off.The microbial method removal to difficult decomposing organic matter Poor effect.
Summary of the invention
The problem existed for above-mentioned prior art, the present invention provides the preparation side of a kind of modified pearl rock biologic packing material Method, the method comprises the steps:
(1), preparation modification liquid (in parts by weight):
N hydroxymethyl acrylamide 40-60
3-chloro-2-hydroxypropyl trimethylammonium amine hydrochlorate 15-30
Triethanolamine 60-90
Polyvinyl alcohol 12-18
Acrylic acid methyl ester. 6-8
MgCl2 10-15
Water 100-150;
(2), the preparation of modified pearl rock: taking the modification liquid of 500g part step (1), then adding 150g granularity is 500 mesh Perlite, continuously stirred 30 minutes, after perlite scatter, be warming up to 80 DEG C, stir 1-3h at such a temperature, filter To solid matter be washed with deionized 3 times after be dried, dried pressed powder is placed in Muffle furnace in air atmosphere 400 DEG C of roastings 3 hours, obtain modified pearl rock;
(3), the preparation of Ti/Zn-ZSM-5: take Titanium Nitrate and zinc nitrate is dissolved in the deionized water of 250ml, wherein nitric acid The gross weight of titanium and zinc nitrate is 1.625g, standby after stirring and dissolving;ZSM-5 is dispersed in ammonia spirit, keeps at 100 DEG C 5 hours, it is dried after filtration, dried powder 15g is added in the solution of above-mentioned Titanium Nitrate and zinc nitrate, be then warming up to 80 DEG C, Constant temperature stirs 20 hours, filters and dry solids, obtains the modified ZSM-5 of Ti and Zn;
(4), take Ti/Zn-ZSM-5 and modified pearl rock that mass ratio is 3:1, add in agitator, then add appropriate Ludox and distilled water, pull an oar, filter after be dried, after 450 DEG C of roasting 0.5h, broken sieve, take the granule conduct of 50-100 mesh Filling carrier;
(5), by mixed bacteria liquid uniform application on the filling carrier surface that step (4) obtains, repaste and smear mass percent It is the sodium alginate soln of 0.5%, by clean water after placing about 2-4 hour, is dried 20 hours at 40 DEG C, obtain modified raw Thing filler.
In step (5), the preparation method of mixed bacteria liquid is as follows:
Culture medium is utilized to filter out Paracoccus denitrificans, Bacillus licheniformis, sticky red rhodotorula and wax from water body or mud Shape bacillus cereus;
Paracoccus denitrificans, Bacillus licheniformis, sticky red rhodotorula and Bacillus cercus are respectively connected to Carnis Bovis seu Bubali cream albumen Peptone fluid medium, after 36 DEG C are cultivated 24h, centrifugal 10min under the rotating speed of 5000rpm, by raw for the solid sediment obtained Resuspended 3 times of reason saline, obtains four kinds of bacteria suspensions;
By the bacteria suspension of Paracoccus denitrificans, the bacteria suspension of Bacillus licheniformis, the bacteria suspension of sticky red rhodotorula and wax-like bud The bacteria suspension of spore bacillus is mixed to get microorganism species by the volume ratio of 1: 1: 1: 2, accesses in culture medium and obtains connecing bacterium amount 8% Plastc ring;
Plastc ring by the Paracoccus denitrificans obtained, Bacillus licheniformis, sticky red rhodotorula and Bacillus cercus Putting in container, the pH value being diluted to mixed liquor with deionized water is neutrality, adds polyvinyl alcohol and forms mixed bacteria liquid.
Preparation modification liquid (in parts by weight) in step (1):
N hydroxymethyl acrylamide 50
3-chloro-2-hydroxypropyl trimethylammonium amine hydrochlorate 20
Triethanolamine 75
Polyvinyl alcohol 16
Acrylic acid methyl ester. 7
MgCl2 12.5
Water 125.
It is an advantage of the current invention that:
(1) N hydroxymethyl acrylamide in modification liquid, triethanolamine, polyvinyl alcohol, acrylic acid methyl ester., MgCl2Occur Cooperative effect, the perlite of the present invention liquid that is modified is modified, improves the degree of roughness on surface, further increases filler Specific surface area and the bond area of microorganism so that it is there is excellent microorganism colonization performance;
(2) the modified perlite carrier of the liquid that is modified has the absorption of higher long chain alkane and polycyclic aromatic hydrocarbon yield and divides Solution ability;
(3) modified perlite filler carrier mechanical property is obviously improved;
(4) present invention use perlite simple as low raw-material cost, rich reserves, and production technology, with micro- Biological compound tense, does not interferes with the normal growth of microorganism, substantially without microorganism brings murder by poisoning reaction, avoids simultaneously yet Water body is caused secondary pollution.
Detailed description of the invention
Below in conjunction with embodiment, the invention will be further described.
First preparing mixed bacteria liquid, concrete grammar is as follows:
Culture medium is utilized to filter out Paracoccus denitrificans, Bacillus licheniformis, sticky red rhodotorula and wax from water body or mud Shape bacillus cereus;
Paracoccus denitrificans, Bacillus licheniformis, sticky red rhodotorula and Bacillus cercus are respectively connected to Carnis Bovis seu Bubali cream albumen Peptone fluid medium, after 36 DEG C are cultivated 24h, centrifugal 10min under the rotating speed of 5000rpm, by raw for the solid sediment obtained Resuspended 3 times of reason saline, obtains four kinds of bacteria suspensions;
By the bacteria suspension of Paracoccus denitrificans, the bacteria suspension of Bacillus licheniformis, the bacteria suspension of sticky red rhodotorula and wax-like bud The bacteria suspension of spore bacillus is mixed to get microorganism species by the volume ratio of 1: 1: 1: 2, accesses in culture medium and obtains connecing bacterium amount 8% Plastc ring;
Plastc ring by the Paracoccus denitrificans obtained, Bacillus licheniformis, sticky red rhodotorula and Bacillus cercus Putting in container, the pH value being diluted to mixed liquor with deionized water is neutrality, adds polyvinyl alcohol and forms mixed bacteria liquid.
Embodiment 1
(1), preparation modification liquid (in parts by weight):
N hydroxymethyl acrylamide 40
3-chloro-2-hydroxypropyl trimethylammonium amine hydrochlorate 15
Triethanolamine 60
Polyvinyl alcohol 12
Acrylic acid methyl ester. 6
MgCl2 10
Water 100;
(2), the preparation of modified pearl rock: taking the modification liquid of 500g part step (1), then adding 150g granularity is 500 mesh Perlite, continuously stirred 30 minutes, after perlite scatter, be warming up to 80 DEG C, stir 1h at such a temperature, be filtrated to get Solid matter be washed with deionized 3 times after be dried, dried pressed powder is placed in Muffle furnace in air atmosphere 400 DEG C roasting 3 hours, obtains modified pearl rock;
(3), the preparation of Ti/Zn-ZSM-5: take Titanium Nitrate and zinc nitrate is dissolved in the deionized water of 250ml, wherein nitric acid The gross weight of titanium and zinc nitrate is 1.625g, standby after stirring and dissolving;ZSM-5 is dispersed in ammonia spirit, keeps at 100 DEG C 5 hours, it is dried after filtration, dried powder 15g is added in the solution of above-mentioned Titanium Nitrate and zinc nitrate, be then warming up to 80 DEG C, Constant temperature stirs 20 hours, filters and dry solids, obtains the modified ZSM-5 of Ti and Zn;
(4), take Ti/Zn-ZSM-5 and modified pearl rock that mass ratio is 3:1, add in agitator, then add appropriate Ludox and distilled water, pull an oar, filter after be dried, after 450 DEG C of roasting 0.5h, broken sieve, take the granule conduct of 50-100 mesh Filling carrier;
(5), by mixed bacteria liquid uniform application on the filling carrier surface that step (4) obtains, repaste and smear mass percent It is the sodium alginate soln of 0.5%, by clean water after placing about 2 hours, is dried 20 hours at 40 DEG C, obtains modification biological Filler.
The modified interface shear strength of this biologic packing material improves 38%, and microorganism colonization amount improves 30%, how long alkane The clearance of hydrocarbon reaches 98%, and the clearance of polycyclic aromatic hydrocarbon is reached 99%.
Embodiment 2
(1), preparation modification liquid (in parts by weight):
N hydroxymethyl acrylamide 40-60
3-chloro-2-hydroxypropyl trimethylammonium amine hydrochlorate 15-30
Triethanolamine 60-90
Polyvinyl alcohol 12-18
Acrylic acid methyl ester. 6-8
MgCl2 10-15
Water 100-150;
(2), the preparation of modified pearl rock: taking the modification liquid of 500g part step (1), then adding 150g granularity is 500 mesh Perlite, continuously stirred 30 minutes, after perlite scatter, be warming up to 80 DEG C, stir 1-3h at such a temperature, filter To solid matter be washed with deionized 3 times after be dried, dried pressed powder is placed in Muffle furnace in air atmosphere 400 DEG C of roastings 3 hours, obtain modified pearl rock;
(3), the preparation of Ti/Zn-ZSM-5: take Titanium Nitrate and zinc nitrate is dissolved in the deionized water of 250ml, wherein nitric acid The gross weight of titanium and zinc nitrate is 1.625g, standby after stirring and dissolving;ZSM-5 is dispersed in ammonia spirit, keeps at 100 DEG C 5 hours, it is dried after filtration, dried powder 15g is added in the solution of above-mentioned Titanium Nitrate and zinc nitrate, be then warming up to 80 DEG C, Constant temperature stirs 20 hours, filters and dry solids, obtains the modified ZSM-5 of Ti and Zn;
(4), take Ti/Zn-ZSM-5 and modified pearl rock that mass ratio is 3:1, add in agitator, then add appropriate Ludox and distilled water, pull an oar, filter after be dried, after 450 DEG C of roasting 0.5h, broken sieve, take the granule conduct of 50-100 mesh Filling carrier;
(5), by mixed bacteria liquid uniform application on the filling carrier surface that step (4) obtains, repaste and smear mass percent It is the sodium alginate soln of 0.5%, by clean water after placing about 2-4 hour, is dried 20 hours at 40 DEG C, obtain modified raw Thing filler.
This biologic packing material magnesium oxide modified rear interface shear strength improves 51%, and microorganism colonization amount improves 41%, many The clearance of long chain alkane reaches 99%, and the clearance of polycyclic aromatic hydrocarbon is reached 99%.
Embodiment 3
(1), preparation modification liquid (in parts by weight):
N hydroxymethyl acrylamide 60
3-chloro-2-hydroxypropyl trimethylammonium amine hydrochlorate 30
Triethanolamine 90
Polyvinyl alcohol 18
Acrylic acid methyl ester. 8
MgCl2 15
Water 150;
(2), the preparation of modified pearl rock: taking the modification liquid of 500g part step (1), then adding 150g granularity is 500 mesh Perlite, continuously stirred 30 minutes, after perlite scatter, be warming up to 80 DEG C, stir 3h at such a temperature, be filtrated to get Solid matter be washed with deionized 3 times after be dried, dried pressed powder is placed in Muffle furnace in air atmosphere 400 DEG C roasting 3 hours, obtains modified pearl rock;
(3), the preparation of Ti/Zn-ZSM-5: take Titanium Nitrate and zinc nitrate is dissolved in the deionized water of 250ml, wherein nitric acid The gross weight of titanium and zinc nitrate is 1.625g, standby after stirring and dissolving;ZSM-5 is dispersed in ammonia spirit, keeps at 100 DEG C 5 hours, it is dried after filtration, dried powder 15g is added in the solution of above-mentioned Titanium Nitrate and zinc nitrate, be then warming up to 80 DEG C, Constant temperature stirs 20 hours, filters and dry solids, obtains the modified ZSM-5 of Ti and Zn;
(4), take Ti/Zn-ZSM-5 and modified pearl rock that mass ratio is 3:1, add in agitator, then add appropriate Ludox and distilled water, pull an oar, filter after be dried, after 450 DEG C of roasting 0.5h, broken sieve, take the granule conduct of 50-100 mesh Filling carrier;
(5), by mixed bacteria liquid uniform application on the filling carrier surface that step (4) obtains, repaste and smear mass percent It is the sodium alginate soln of 0.5%, by clean water after placing about 4 hours, is dried 20 hours at 40 DEG C, obtains modification biological Filler.
This biologic packing material magnesium oxide modified rear interface shear strength improves 60%, and microorganism colonization amount improves 50%, many The clearance of long chain alkane reaches 99%, and the clearance of polycyclic aromatic hydrocarbon is reached 99.5%.

Claims (3)

1. the preparation method of a modified pearl rock biologic packing material, it is characterised in that the method comprises the steps:
(1), modification liquid is prepared, in parts by weight:
N hydroxymethyl acrylamide 40-60
3-chloro-2-hydroxypropyl trimethylammonium amine hydrochlorate 15-30
Triethanolamine 60-90
Polyvinyl alcohol 12-18
Acrylic acid methyl ester. 6-8
MgCl2 10-15
Water 100-150;
(2), the preparation of modified pearl rock: take the modification liquid of 500g part step (1), then add the treasure that 150g granularity is 500 mesh Zhu Yan, continuously stirred 30 minutes, after perlite scatter, is warming up to 80 DEG C, stirs 1-3h at such a temperature, be filtrated to get Solid matter is dried after being washed with deionized 3 times, and dried pressed powder is placed in Muffle furnace in air atmosphere 400 DEG C Roasting 3 hours, obtains modified pearl rock;
(3), the preparation of Ti/Zn-ZSM-5: take Titanium Nitrate and zinc nitrate be dissolved in the deionized water of 250ml, wherein Titanium Nitrate and The gross weight of zinc nitrate is 1.625g, standby after stirring and dissolving;Being dispersed in ammonia spirit by ZSM-5, at 100 DEG C, holding 5 is little Time, it is dried after filtration, dried powder 15g is added in the solution of above-mentioned Titanium Nitrate and zinc nitrate, be then warming up to 80 DEG C, constant temperature Stir 20 hours, filter and dry solids, obtain the modified ZSM-5 of Ti and Zn;
(4), take Ti/Zn-ZSM-5 and modified pearl rock that mass ratio is 3:1, add in agitator, then add proper amount of silicon molten Glue and distilled water, pull an oar, filter after be dried, after 450 DEG C of roasting 0.5h, broken sieve, take the granule of 50-100 mesh as filler Carrier;
(5), by mixed bacteria liquid uniform application on the filling carrier surface that step (4) obtains, repaste and smear mass percent and be The sodium alginate soln of 0.5%, by clean water after placing 2-4 hour, is dried 20 hours at 40 DEG C, obtains modification biological and fill out Material.
2. preparation method as claimed in claim 1, it is characterised in that in step (5), the preparation method of mixed bacteria liquid is as follows:
Culture medium is utilized to filter out Paracoccus denitrificans, Bacillus licheniformis and sticky red rhodotorula and wax-like bud from water body or mud Spore bacillus;
Paracoccus denitrificans, Bacillus licheniformis, sticky red rhodotorula and Bacillus cercus are respectively connected to beef extract-peptone liquid Body culture medium, after 36 DEG C are cultivated 24h, centrifugal 10min, the solid sediment physiology salt that will obtain under the rotating speed of 5000rpm Resuspended 3 times of water, obtains four kinds of bacteria suspensions;
By the bacteria suspension of Paracoccus denitrificans, the bacteria suspension of Bacillus licheniformis, the bacteria suspension of sticky red rhodotorula and wax-like spore bar The bacteria suspension of bacterium is mixed to get microorganism species by the volume ratio of 1: 1: 1: 2, accesses in culture medium and is mixed connecing bacterium amount 8% Bacteria suspension;
The plastc ring of the Paracoccus denitrificans obtained, Bacillus licheniformis, sticky red rhodotorula and Bacillus cercus is put into In container, the pH value being diluted to mixed liquor with deionized water is neutrality, adds polyvinyl alcohol and forms mixed bacteria liquid.
3. preparation method as claimed in claim 1, it is characterised in that preparation modification liquid in step (1), in parts by weight:
N hydroxymethyl acrylamide 50
3-chloro-2-hydroxypropyl trimethylammonium amine hydrochlorate 20
Triethanolamine 75
Polyvinyl alcohol 16
Acrylic acid methyl ester. 7
MgCl2 12.5
Water 125.
CN201510155211.7A 2015-04-03 2015-04-03 A kind of preparation method of modified pearl rock biologic packing material Expired - Fee Related CN104743680B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201510155211.7A CN104743680B (en) 2015-04-03 2015-04-03 A kind of preparation method of modified pearl rock biologic packing material

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201510155211.7A CN104743680B (en) 2015-04-03 2015-04-03 A kind of preparation method of modified pearl rock biologic packing material

Publications (2)

Publication Number Publication Date
CN104743680A CN104743680A (en) 2015-07-01
CN104743680B true CN104743680B (en) 2016-11-30

Family

ID=53584051

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201510155211.7A Expired - Fee Related CN104743680B (en) 2015-04-03 2015-04-03 A kind of preparation method of modified pearl rock biologic packing material

Country Status (1)

Country Link
CN (1) CN104743680B (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107903769A (en) * 2017-11-30 2018-04-13 明光市裕阳新材料有限公司 A kind of heat-insulation and heat-preservation interior wall coating and preparation method thereof
CN109354307B (en) * 2018-11-01 2021-10-01 山东尚科环境工程有限公司 In-situ purification black and odorous water body treatment method and treatment device thereof
CN109354331A (en) * 2018-12-11 2019-02-19 辽宁中舟得水环保科技有限公司 A kind of method of biologic packing material anaerobic technique processing industrial wastewater
CN112495337B (en) * 2020-11-11 2022-06-14 常熟理工学院 Method for preparing ceramsite filter material by using oil-containing sludge
CN113698988B (en) * 2021-07-27 2024-04-26 长寿花食品股份有限公司 Production process of nutritional corn oil

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1587108A (en) * 2004-09-23 2005-03-02 上海交通大学 Sewage deep treatment filler with physiochemical adsorption and biological film property
CN102626606A (en) * 2011-12-27 2012-08-08 北京大学 Modified zeolite filling material BMZ-1 and its preparation method and application
CN102897899A (en) * 2012-09-24 2013-01-30 哈尔滨工业大学 Modified biological filler for wastewater treatment and preparation method thereof

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61140985A (en) * 1984-12-14 1986-06-28 株式会社日立製作所 Character code conversion system

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1587108A (en) * 2004-09-23 2005-03-02 上海交通大学 Sewage deep treatment filler with physiochemical adsorption and biological film property
CN102626606A (en) * 2011-12-27 2012-08-08 北京大学 Modified zeolite filling material BMZ-1 and its preparation method and application
CN102897899A (en) * 2012-09-24 2013-01-30 哈尔滨工业大学 Modified biological filler for wastewater treatment and preparation method thereof

Also Published As

Publication number Publication date
CN104743680A (en) 2015-07-01

Similar Documents

Publication Publication Date Title
CN104743680B (en) A kind of preparation method of modified pearl rock biologic packing material
Geng et al. Enhanced aerobic sludge granulation in a Sequencing Batch Reactor (SBR) by applying mycelial pellets
CN102527347B (en) Magnetic chitosan/cationic surface active agent modified zeolite adsorbent and preparation method and application thereof
CN112657465B (en) Preparation method of magnetic biochar and method for treating tailing wastewater
CN109382075B (en) Composite microbial bacterium adsorbent, preparation method thereof and sewage treatment method
CN106277283B (en) Strengthen the method for removing arsenic antimony ion in water removal using iron and manganese oxides biological in filter tank
CN107641609B (en) Method for preparing flocculating agent by using compound microbial inoculum
CN108018280A (en) A kind of construction method of microorganism slow-released carrier
CN104773817B (en) A kind of preparation method of modification biological filler
Allam Bioremediation efficiency of heavy metals and azo dyes by individual or consortium bacterial species either as free orimmobilized cells: a comparative Study
CN104649420A (en) Method of removing nitrate nitrogen in water body
CN1681916A (en) Heavy metal adsorbent composition
CN109110863A (en) Utilize the method for fortimicin in chemical activation/micro-wave digestion activation biological carbon materials removal water body
CN111944799A (en) Preparation method and application of immobilized particles embedded with thiobacillus denitrificans
CN104528954B (en) A kind of preparation method of degradable composite biological packing
CN107619802A (en) The cold bacillus in one plant of ocean and prepare the method for flocculant with it
CN107099483B (en) Composite biological agent and application thereof in treatment of mercury-containing wastewater
CN104342373B (en) Microalgae culture solution treatment method
CN108993425A (en) A kind of compound biological adsorption agent and its application
CN1732053A (en) Method for removing heavy metal in incineration ash
CN116589130A (en) Purification treatment method of seawater for aquaculture
CN108017159B (en) Ecological bionic microbial flocculant based on ruditapes philippinarum adhered sludge and preparation method thereof
CN107446912B (en) Immobilized saccharomyces cerevisiae engineering bacteria and method for removing heavy metal Pb in wastewater by using same2+In (1)
CN107236684A (en) The application of Oneida Shewanella and its immobilized spherule in processing dyeing waste water
CN110980967A (en) Microbial composite water purifying agent and preparation method thereof

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C41 Transfer of patent application or patent right or utility model
CB03 Change of inventor or designer information

Inventor after: Li Wenxin

Inventor after: Di Junlv

Inventor before: Li Wenxin

COR Change of bibliographic data
TA01 Transfer of patent application right

Effective date of registration: 20161019

Address after: Zhejiang province Jinhua City Xiguan Street 321000 Wucheng Dong Zhai community Dong Kang Lane 6, Room 203

Applicant after: JINHUA ZHICHAN WUYUAN INFORMATION TECHNOLOGY CO., LTD.

Applicant after: Chen Dongdong

Address before: A village of 311800 Zhejiang city in Shaoxing Province, Zhuji City, East Street Licun

Applicant before: Li Wenxin

C14 Grant of patent or utility model
GR01 Patent grant
CB03 Change of inventor or designer information

Inventor after: Li Jianzhen

Inventor before: Li Wenxin

Inventor before: Di Junlv

CB03 Change of inventor or designer information
TR01 Transfer of patent right

Effective date of registration: 20171017

Address after: 061303 Cangzhou, Hebei, Yanshan, Han Town, Licun No. 76

Patentee after: Li Jianzhen

Address before: Zhejiang province Jinhua City Xiguan Street 321000 Wucheng Dong Zhai community Dong Kang Lane 6, Room 203

Co-patentee before: Chen Dongdong

Patentee before: JINHUA ZHICHAN WUYUAN INFORMATION TECHNOLOGY CO., LTD.

TR01 Transfer of patent right
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20161130

Termination date: 20180403

CF01 Termination of patent right due to non-payment of annual fee