CN107439685A - A kind of preparation method of Quercetin health care yoghourt - Google Patents

A kind of preparation method of Quercetin health care yoghourt Download PDF

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Publication number
CN107439685A
CN107439685A CN201710763488.7A CN201710763488A CN107439685A CN 107439685 A CN107439685 A CN 107439685A CN 201710763488 A CN201710763488 A CN 201710763488A CN 107439685 A CN107439685 A CN 107439685A
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CN
China
Prior art keywords
quercetin
health care
preparation
care yoghourt
emulsion
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Pending
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CN201710763488.7A
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Chinese (zh)
Inventor
郭秀兰
唐仁勇
王国泽
卢玉荣
罗静
任荣
谭浩
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Chengdu University
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Chengdu University
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Priority to CN201710763488.7A priority Critical patent/CN107439685A/en
Publication of CN107439685A publication Critical patent/CN107439685A/en
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00Milk preparations; Milk powder or milk powder preparations
    • A23C9/12Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/13Fermented milk preparations; Treatment using microorganisms or enzymes using additives

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  • Life Sciences & Earth Sciences (AREA)
  • Microbiology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

The invention discloses a kind of preparation method of Quercetin health care yoghourt, comprising preparation raw material, prepares emulsion, homogeneous, sterilizing, inoculation, fermentation and after fermentation.The present invention solves the problems, such as this technological difficulties that Quercetin is insoluble in water, can be applied in Yoghourt, develops a Quercetin health care yoghourt, suitable for promoting.

Description

A kind of preparation method of Quercetin health care yoghourt
Technical field
The present invention relates to technical field of dairy products, is more precisely related to a kind of making side of Quercetin health care yoghourt Method.
Background technology
Quercetin is a kind of polyhydroxy flavone compound, is widely present in daily various vegetables and fruit.Quercitrin Element has various biological activity, and substantial amounts of research at present has shown that Quercetin has the function that anti-oxidant and removes free radical, Anti-inflammatory, anti-oxidant, antitumor, platelet aggregation-against, hypoglycemic, regulation blood fat, expansion blood vessel etc. have obvious action, Through being considered as one of most promising diet medicine for being used to prevent and treat a variety of chronic diseases.Abroad Quercetin by One of main component of functional food is included in, according to the research of University of Maryland medical center, healthy adult daily should 100-250mg Quercetins are taken in, and the Quercetin that people take in from daily Chinese style diet is about daily 25mg, it is also necessary to mend Filling substantial amounts of Quercetin could meet that body maintains healthy and prophylactic needs.
As people are also continuously increased to healthy and prophylactic attention, market to the demand of health food, Yoghourt is just It is the product that one of which is nutritious and is liked by consumers in general, especially health care yoghourt.And it is related to Quercetin Health care yoghourt research is less, and it is relevant that this may be insoluble in water with Quercetin, it is difficult to it is applied in liquid or semisolid food, The not no health care yoghourt containing Quercetin on the market at present.Want a kind of functional health Yoghourt containing Quercetin is made, this Exactly need to overcome Quercetin this technological difficulties not soluble in water, Quercetin coating can be dissolved in water and then add coating buffer Fermented into Yoghourt, so as to obtain the health care yoghourt containing Quercetin.
Therefore, how to solve the above problems becomes the direction of those skilled in the art's effort.
The content of the invention
The problem of existing for prior art, the invention provides a kind of preparation method of Quercetin health care yoghourt.
To achieve these goals, the technical solution adopted by the present invention is:
A kind of preparation method of Quercetin health care yoghourt, it is as follows comprising step,
1. prepares raw material:Primary raw material includes milk powder, xylitol and Quercetin coating buffer;
2. prepares emulsion:Milk powder 120g-130g is weighed, 750ml-850ml warm waters, warm water temperature are added in milk powder Degree is maintained at 70 DEG C -80 DEG C, after milk powder is dissolved in warm water, adds 75g-85g xylitols and 190ml-210ml quercitrin Plain coating buffer, it is mixed the emulsion for uniformly, being configured to 940ml-1060ml;
3. homogeneous:By step 2. in emulsion on homogenizer homogeneous 1min-2min, the pressure of homogenizer be maintained at 9MPa-11MPa;
4. sterilizes:Emulsion after step 3. middle homogeneous is incubated to 9min-10min progress in 88 DEG C -92 DEG C of water-bath Sterilization processing, 38 DEG C -42 DEG C are then cooled to, so as to obtain feed liquid;
5. is inoculated with:Aseptically, by leavening, be inoculated into according to 1g/1000ml ratio in feed liquid, and by its Stir, so as to obtain being inoculated with feed liquid;
6. ferments:Aseptically, inoculation feed liquid equivalent filling in the vial of sterilizing and is sealed, then will The vial sealed is positioned in 42 DEG C of constant incubators the 4h-5h that ferments;
7. after fermentation:Vial after step 6. middle fermentation is put into 1 DEG C -4 DEG C of refrigerator and carries out after fermentation, rear hair Ferment at least keeps 24h, so as to which Quercetin health care yoghourt finished product be made.
Preferably, step 1. middle Quercetin coating buffer preparation method include step it is as follows,
(1) prepares the cyclodextrin aqueous solution that concentration is 0.05mol/L, and equimolar is added in the cyclodextrin aqueous solution Quercetin, obtain material solution;
(2) stirs and evenly mixs the material solution in step (1), then allows material solution to allow within static at least 1 hour it to clarify, Obtain clarified solution;
(3) clarified solution in step (2) is filtered and determines the polyphenol content in filtrate by, and polyphenol content is 0.70mg/ml, so as to obtain Quercetin coating buffer.
Preferably, material solution is stirred using magnetic stirring apparatus in step (2).
Preferably, using FoLin-CiocaLteu methods measure polyphenol content in step (3).
Preferably, 1. middle milk powder uses high-quality skimmed milk power, wherein CP≤33%, CHO≤54%, EE≤1% to step.
Preferably, Dan white matter Han Liang≤4% of the step 2. emulsion of middle gained.
Preferably, step 5. in leavening be the leavening containing lactobacillus bulgaricus and streptococcus thermophilus.
Beneficial effect:The invention provides a kind of preparation method of Quercetin health care yoghourt, solve Quercetin and be insoluble in This technological difficulties problem of water, can be applied in Yoghourt, a Quercetin health care yoghourt be developed, suitable for pushing away Extensively.
Embodiment
A kind of preparation method of Quercetin health care yoghourt, it is as follows comprising step,
1. prepares raw material:Primary raw material includes milk powder, xylitol and Quercetin coating buffer, and milk powder uses high-quality defatted milk Powder, wherein CP (crude protein)≤33%, CHO (starch)≤54%, EE (crude fat)≤1%;
2. prepares emulsion:Milk powder 120g-130g (preferably 125g) is weighed, 750ml-850ml is added (preferably in milk powder 800ml) warm water, warm water temperature is maintained at 70 DEG C -80 DEG C, and after milk powder is dissolved in warm water, it is (excellent to add 75g-85g Select 80g) xylitol and 190ml-210ml (preferably 200ml) Quercetin coating buffer, it is mixed uniformly, is configured to 940ml- 1060ml (preferably 1000ml) emulsion, Dan white matter Han Liang≤4% of emulsion;
3. homogeneous:By step 2. in emulsion on homogenizer homogeneous 1min-2min, the pressure of homogenizer be maintained at 9MPa-11Mpa (preferably 10Mpa);
4. sterilizes:Emulsion after step 3. middle homogeneous is incubated in the water-bath of 88 DEG C -92 DEG C (preferably 90 DEG C) 9min-10min (preferably 10min) carries out sterilization processing, 38 DEG C -42 DEG C (preferably 40 DEG C) is then cooled to, so as to obtain Feed liquid;
5. is inoculated with:Aseptically, by the leavening containing lactobacillus bulgaricus and streptococcus thermophilus, according to 1g/ 1000ml ratio is inoculated into feed liquid, and is stirred for uniformly, so as to obtain being inoculated with feed liquid;
6. ferments:Aseptically, inoculation feed liquid equivalent filling in the vial of sterilizing and is sealed, then will The vial sealed is positioned in 42 DEG C of constant incubators the 4h-5h that ferments, and is just taken out after inoculation feed liquid solidification;
7. after fermentation:Vial after step 6. middle fermentation is put into 1 DEG C -4 DEG C of refrigerator and carries out after fermentation, rear hair Ferment at least keeps 24h, so as to which Quercetin health care yoghourt finished product be made.
The step 1. middle Quercetin coating buffer preparation method include step it is as follows,
(1) prepares the cyclodextrin aqueous solution that concentration is 0.05mol/L, and equimolar is added in the cyclodextrin aqueous solution Quercetin, obtain material solution;
(2) material solution in step (1) is stirred mixing by using magnetic stirring apparatus, then makes material solution static Allow within least 1 hour it to clarify, obtain clarified solution;
(3) clarified solution in step (2) is filtered and uses FoLin-CiocaLteu (forint-Theo Ka Te) method by Polyphenol content in (reagent II i.e. in Folin- phenol reagents) measure filtrate, polyphenol content 0.70mg/ml, so as to obtain Mongolian oak Skin element coating buffer.
The invention provides a kind of preparation method of Quercetin health care yoghourt, solves this skill that Quercetin is insoluble in water It art difficulties, can be applied in Yoghourt, develop a Quercetin health care yoghourt, suitable for promoting.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention All any modification, equivalent and improvement made within refreshing and principle etc., should be included in the scope of the protection.

Claims (7)

  1. A kind of 1. preparation method of Quercetin health care yoghourt, it is characterised in that:It is as follows comprising step,
    1. prepares raw material:Primary raw material includes milk powder, xylitol and Quercetin coating buffer;
    2. prepares emulsion:Milk powder 120g-130g is weighed, 750ml-850ml warm waters are added in milk powder, warm water temperature is protected Hold at 70 DEG C -80 DEG C, after milk powder is dissolved in warm water, add 75g-85g xylitols and 190ml-210ml Quercetin bag By liquid, the emulsion for uniformly, being configured to 940ml-1060ml is mixed;
    3. homogeneous:By step 2. in emulsion on homogenizer homogeneous 1min-2min, the pressure of homogenizer be maintained at 9MPa- 11MPa;
    4. sterilizes:The emulsion after step 3. middle homogeneous is incubated into 9min-10min in 88 DEG C -92 DEG C of water-bath to be sterilized Processing, is then cooled to 38 DEG C -42 DEG C, so as to obtain feed liquid;
    5. is inoculated with:Aseptically, by leavening, it is inoculated into according to 1g/1000ml ratio in feed liquid, and be stirred for Uniformly, so as to obtain be inoculated with feed liquid;
    6. ferments:Aseptically, inoculation feed liquid equivalent filling in the vial of sterilizing and is sealed, then will sealing Good vial is positioned in 42 DEG C of constant incubators the 4h-5h that ferments;
    7. after fermentation:Vial after step 6. middle fermentation is put into 1 DEG C -4 DEG C of refrigerator and carries out after fermentation, after fermentation is extremely 24h is kept less, so as to which Quercetin health care yoghourt finished product be made.
  2. 2. the preparation method of Quercetin health care yoghourt according to claim 1, it is characterised in that:1. middle Quercetin is coated with step The preparation method of liquid is as follows comprising step,
    (1) prepares the cyclodextrin aqueous solution that concentration is 0.05mol/L, and equimolar Mongolian oak is added in the cyclodextrin aqueous solution Pi Su, obtain material solution;
    (2) stirs and evenly mixs the material solution in step (1), then allows material solution to allow within static at least 1 hour it to clarify, and obtains Clarified solution;
    (3) clarified solution in step (2) is filtered and determines the polyphenol content in filtrate by, polyphenol content 0.70mg/ Ml, so as to obtain Quercetin coating buffer.
  3. 3. the preparation method of Quercetin health care yoghourt according to claim 2, it is characterised in that:Magnetic force is used in step (2) Agitator is stirred to material solution.
  4. 4. the preparation method of Quercetin health care yoghourt according to claim 2, it is characterised in that:Used in step (3) FoLin-CiocaLteu methods determine polyphenol content.
  5. 5. the preparation method of Quercetin health care yoghourt according to claim 1, it is characterised in that:Step 1. middle milk powder using excellent Matter skimmed milk power, wherein CP≤33%, CHO≤54%, EE≤1%.
  6. 6. the preparation method of Quercetin health care yoghourt according to claim 1, it is characterised in that:The emulsion of step 2. middle gained Dan white matter Han Liang≤4%.
  7. 7. the preparation method of Quercetin health care yoghourt according to claim 1, it is characterised in that:Step 5. in leavening be Leavening containing lactobacillus bulgaricus and streptococcus thermophilus.
CN201710763488.7A 2017-08-30 2017-08-30 A kind of preparation method of Quercetin health care yoghourt Pending CN107439685A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114423420A (en) * 2019-09-24 2022-04-29 捷通国际有限公司 Methods and compositions for producing phytonutrients in microbiome

Citations (4)

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Publication number Priority date Publication date Assignee Title
CN103609693A (en) * 2013-11-13 2014-03-05 王玉清 Astragalus membranaceus health-care yogurt and manufacture method thereof
CN104413148A (en) * 2013-08-28 2015-03-18 青岛医防消毒专业技术中心 Water lily flower yogurt product
CN105795438A (en) * 2014-04-16 2016-07-27 南昌大学 Method for reducing generation of furan in soybean milk powder made through spray drying
CN106343018A (en) * 2016-08-27 2017-01-25 湖北文理学院 Radix ophiopogonis health-care yogurt and preparation method thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104413148A (en) * 2013-08-28 2015-03-18 青岛医防消毒专业技术中心 Water lily flower yogurt product
CN103609693A (en) * 2013-11-13 2014-03-05 王玉清 Astragalus membranaceus health-care yogurt and manufacture method thereof
CN105795438A (en) * 2014-04-16 2016-07-27 南昌大学 Method for reducing generation of furan in soybean milk powder made through spray drying
CN106343018A (en) * 2016-08-27 2017-01-25 湖北文理学院 Radix ophiopogonis health-care yogurt and preparation method thereof

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Title
崔山风等: "《槲皮素包合物的制备工艺及增溶作用研究》", 《中国药师》 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114423420A (en) * 2019-09-24 2022-04-29 捷通国际有限公司 Methods and compositions for producing phytonutrients in microbiome

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Application publication date: 20171208