CN107439616A - A kind of natural environmental-protective type pool-cleaning agent and preparation method thereof - Google Patents
A kind of natural environmental-protective type pool-cleaning agent and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/28—Myrtaceae [Myrtle family], e.g. teatree or clove
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
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- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The present invention relates to a kind of natural environmental-protective type pool-cleaning agent and preparation method thereof, the pool-cleaning agent includes following raw material：The general bacterium MDM nutrient solutions of Chinese medicine extract, pineapple, water hyacinth extract solution, cosolvent, thickener, preparation method are：(1) the general bacterium MDM nutrient solutions of Chinese medicine extract, pineapple, water hyacinth extract solution are added in stainless steel first, are added cosolvent, are stirred；(2) thickener is added into step (1), stir to obtain mixed liquor；(3) above-mentioned mixed liquor is made to the pool-cleaning agent of solid type of spray dryer, the invention provides pool-cleaning agent easy to process, that recovery rate is high, selectivity is strong, effect on environment is minimum.
Description
Technical field
Cleaned up the pond field the invention belongs to pool-cleaning agent preparation field, especially aquaculture, and in particular to a kind of natural environmental-protective
Type pool-cleaning agent and preparation method thereof.
Background technology
With the quickening of agricultural sector structure adjustment paces, the structure of aquaculture kind is more rational, the special type such as shrimp, crab
Aquaculture industry fast development, it has also become one of developing rural economy, the important industry increased farmers' income.In aquaculture
In, it is a very crucial program to clean up the pond, clean up the pond often because breed variety different, multiple disease difference and medicine
The facility in source, and using different types of medicine of cleaning up the pond, the medicine of cleaning up the pond of predator fish is pentachloro- in traditional killing shrimp pool
Phenol sodium, it has larger toxicity, has paralysis nerve to the higher mammal such as shellfish and hemoglobinated aquatic products and people and animals, destroys
The effect of hemoglobin function, and breeding water body and environment are caused seriously to pollute.
In aquaculture, pool-cleaning agent be mainly used in remove wild fishes, tadpole, aquatic insect, snail, aquatic algae,
Pathogenic parasite and other pathogens etc..
Problems be present in existing pool-cleaning agent：
(1) it is serious for people and animals' high poison, environmental pollution；
(2) selectivity is sterilized, function is single, effect is unstable.
The content of the invention
In order to solve the above technical problems, the invention provides it is easy to process, recovery rate is high, selectivity is strong, effect on environment
Minimum a kind of natural environmental-protective type pool-cleaning agent and preparation method thereof.
To achieve these goals, the present invention is achieved by the following technical programs:
A kind of natural environmental-protective type pool-cleaning agent, is made up of the raw material of following percentage by weight：Chinese medicine extract 15~40%, spinach
The general bacterium MDM nutrient solutions 14~35% of trailing plants, water hyacinth extract solution 15~25%, cosolvent 8~19%, thickener 10~19%；
Preferably, the Chinese medicine extract is the fruit of Rangoon creeper, eucalyptus robusta and the taste Chinese medicine extract of chenopodium ambrosiodies 3；
Preferably, the pool-cleaning agent, it is made up of the raw material of following percentage by weight：The general bacterium of Chinese medicine extract 28%, pineapple
MDM nutrient solutions 25%, water hyacinth extract solution 18%, cosolvent 13%, thickener 16%；
1. it is preferable, the preparation method of the Chinese medicine extract, comprise the following steps：
(1) 10~20 parts of the fruit of Rangoon creeper, 5~16 parts of eucalyptus robusta, 11~25 parts of the taste medicine of chenopodium ambrosiodies 3 are put into pot,
The water of 8~10 times of amounts of medicinal material total amount is added, after soaking 20~40min, heating boils 0.5~1h, filters, obtains first extract；
(2) water of 4~6 times of amounts of medicinal material total amount is added into the dregs of a decoction filtered out, heating boils 0.5~1h, filters, obtains secondary
Extract solution；
(3) first extract and secondary raffinate are mixed, stirred, produce the Chinese medicine extract；
Preferably, the preparation method of the general bacterium MDM nutrient solutions of described pineapple is：
(1) the general bacterium MDM of pineapple is seeded in 4L~6L YEB fluid nutrient mediums, in 35~38 DEG C, 200rpm concussion trainings
20~25h is supported, obtains seed liquor；
(2) above-mentioned seed liquor is pressed to 1% inoculum concentration, is forwarded in 300L YEB culture mediums, 35~38 DEG C, 200rpm shakes
10~18h of culture is swung, centrifuging and taking supernatant, produces the general bacterium MDM nutrient solutions of the pineapple；
Preferably, the preparation method of the water hyacinth extract solution comprises the following steps：
(1) fetch water after cucurbit herb is cleaned and drain, dried under nature, dry powder is crushed to obtain with pulverizer；
(2) dry powder of water hyacinth is taken in container, by 1:4 volume ratio adds petroleum ether, extracted at 50~70 DEG C 1h~
2h, filtering, filter residue repeat extraction 3 times, merging filtrate, with Rotary Evaporators rotary evaporation powder with petroleum ether by above-mentioned volume ratio
Shape, it is stand-by；
Preferably, one or more of the cosolvent in ethanol, propane diols, isopropanol；
Preferably, one or more of the thickener in xanthans, carboxymethyl cellulose or urea；
The preparation method of above-mentioned pool-cleaning agent, step are as follows:
(1) fruit of Rangoon creeper, eucalyptus robusta, chenopodium ambrosiodies extract solution, the general bacterium MDM nutrient solutions of pineapple, water hyacinth extract solution are added first
Enter into stainless steel, add cosolvent, stir；
(2) thickener is added into step (1), stir to obtain mixed liquor；
(3) above-mentioned mixed liquor is made to the pool-cleaning agent of solid type of spray dryer.
Beneficial effects of the present invention are:
1st, the general bacterium MDM nutrient solutions of pineapple of pool-cleaning agent of the present invention addition have selectivity it is strong, environmentally safe, to people and animals
The advantages of safe, and phosphorus, ammonia salt etc. are not influenceed in by water body, can be applied to wider environmental field and fast and effeciently be killed
Go out the snails such as Pomacea canaliculata, oncomelania, cuts off its route of transmission, reduces pollution sources, reduces work technique and financial burden.
2nd, contain allelochemical in water hyacinth extract solution, the growth of algae in water can be suppressed using this allelochemical；
Meanwhile water hyacinth extract solution and can enough plays sterilization, antisepsis, especially have to aspergillus niger, Escherichia coli, mould and suppress to imitate
Fruit；It has that effect is good, expense is low, material is naturally easy to get, does not easily cause the excellent of secondary pollution as pool-cleaning agent composition of the present invention
Point.
3rd, the fruit of Rangoon creeper of addition, eucalyptus robusta, chenopodium ambrosiodies extract solution can remove predator fish in the pool, improve fishpond water quality,
And can prevents and treats fish disease；The fruit of Rangoon creeper can kill leech；Trifoliate jewelvine is used to clean up the pond, can kill insect in water, pathogen；Eucalyptus robusta can
Killing the insect in water has antiviral, antibacterium, antimycotic effect, can treat the illnesss such as grass, black carp enteritis, gill rot；
Chenopodium ambrosiodies can prevent and treat fish capillaria and bacteriosis.
Embodiment
To make the purpose, technical scheme and advantage of the embodiment of the present invention clearer, below in conjunction with specific embodiment pair
The present invention is described in detail.It should be pointed out that creative work premise is not being made to those of ordinary skill in the art
Lower obtained every other embodiment, belongs to the scope of protection of the invention.
Embodiment 1:
A kind of preparation method of natural environmental-protective type pool-cleaning agent, comprises the following steps:
(1) first to the raw material of following percentage by weight will be added in stainless steel：The general bacterium of Chinese medicine extract 15%, pineapple
MDM nutrient solutions 30%, water hyacinth extract solution 24%, ethanol 9%, propane diols 7% are added, is stirred；
(2) xanthans 6%, carboxymethyl cellulose 9%, urea 3% are added into step (1), stir to obtain mixed liquor；
(3) above-mentioned mixed liquor is made to the pool-cleaning agent of solid type of spray dryer, the spray drying condition is：Charging
For liquid concentration up to 12 Baume degrees, the Baume degrees is 60 DEG C of Baume degrees measured：180 DEG C of PG-5 type spray dryers inlet temperature,
80 DEG C of outlet temperature, centrifugal head operating pressure 1.8kg/cm2。
The preparation method of above-mentioned Chinese medicine extract, comprises the following steps：
(1) 13 parts of the fruit of Rangoon creeper, 16 parts of eucalyptus robusta, 11 parts of the taste medicine of chenopodium ambrosiodies 3 are put into pot, add medicinal material total amount
The water of 8 times of amounts, after soaking 30min, heating boils 0.5h, filters, obtains first extract；
(2) water of 4 times of amounts of medicinal material total amount is added into the dregs of a decoction filtered out, heating boils 0.5h, filters, obtains second extraction
Liquid；
(3) first extract and secondary raffinate are mixed, stirred, produce the Chinese medicine extract.
The preparation method of the above-mentioned general bacterium MDM nutrient solutions of pineapple, comprises the following steps：
(1) by the general bacterium MDM (CCTCCNO of pineapple:M2014105) be seeded in 5L YEB fluid nutrient mediums, in 35 DEG C,
200rpm concussion and cultivate 22h, obtain seed liquor；
(2) above-mentioned seed liquor is pressed to 1% inoculum concentration, is forwarded in 300L YEB culture mediums, 35 DEG C, 200rpm concussion trainings
18h is supported, centrifuging and taking supernatant, produces the general bacterium MDM nutrient solutions of the pineapple.
The preparation method of above-mentioned water hyacinth extract solution, comprises the following steps：
(1) fetch water after cucurbit herb is cleaned and drain, dried under nature, dry powder is crushed to obtain with pulverizer；
(2) dry powder of water hyacinth is taken in container, by 1:4 volume ratio adds petroleum ether, extracted at 50~70 DEG C 1h~
2h, filtering, filter residue repeat extraction 3 times, merging filtrate, with Rotary Evaporators rotary evaporation powder with petroleum ether by above-mentioned volume ratio
Shape, it is stand-by.
Embodiment 2：
A kind of preparation method of natural environmental-protective type pool-cleaning agent, comprises the following steps:
(1) raw material of following percentage by weight is added into stainless steel first：The general bacterium of Chinese medicine extract 40%, pineapple
MDM nutrient solutions 15%, water hyacinth extract solution 20%, propane diols 5%, isopropanol 7% are added, is stirred；
(2) urea 6%, carboxymethyl cellulose 7% are added into step (1), stir to obtain mixed liquor；
(3) above-mentioned mixed liquor is made to the pool-cleaning agent of solid type of spray dryer, the spray drying condition is：Charging
For liquid concentration up to 15 Baume degrees, the Baume degrees is 60 DEG C of Baume degrees measured：160 DEG C of PG-5 type spray dryers inlet temperature,
90 DEG C of outlet temperature, centrifugal head operating pressure 2.0kg/cm2。
The preparation method of above-mentioned Chinese medicine extract, comprises the following steps：
(1) 20 parts of the fruit of Rangoon creeper, 10 parts of eucalyptus robusta and 14 parts of the taste medicine of chenopodium ambrosiodies 3 are put into pot, it is total adds medicinal material
The water of 7 times of amounts of amount, after soaking 20min, heating boils 0.8h, filters, obtains first extract；
(2) water of 7 times of amounts of medicinal material total amount is added into the dregs of a decoction filtered out, heating boils 0.6h, filters, obtains second extraction
Liquid；
(3) first extract and secondary raffinate are mixed, stirred, produce the Chinese medicine extract.
The preparation method of the above-mentioned general bacterium MDM nutrient solutions of pineapple, comprises the following steps：
(1) by the general bacterium MDM (CCTCCNO of pineapple:M2014105) be seeded in 4L YEB fluid nutrient mediums, in 37 DEG C,
200rpm concussion and cultivate 20h, obtain seed liquor；
(2) above-mentioned seed liquor is pressed to 1% inoculum concentration, is forwarded in 300L YEB culture mediums, 37 DEG C, 200rpm concussion trainings
15h is supported, centrifuging and taking supernatant, produces the general bacterium MDM nutrient solutions of the pineapple.
The preparation method of above-mentioned water hyacinth extract solution, comprises the following steps：
(1) fetch water after cucurbit herb is cleaned and drain, dried under nature, dry powder is crushed to obtain with pulverizer；
(2) dry powder of water hyacinth is taken in container, by 1:4 volume ratio adds petroleum ether, and 1.5h, mistake are extracted at 60 DEG C
Filter, filter residue repeat extraction 3 times by above-mentioned volume ratio with petroleum ether, merging filtrate, with Rotary Evaporators rotary evaporation powdery, treated
With.
Embodiment 3：
A kind of preparation method of natural environmental-protective type pool-cleaning agent, comprises the following steps:
(1) first to the raw material of following percentage by weight will be added in stainless steel：The general bacterium of Chinese medicine extract 33%, pineapple
MDM nutrient solutions 14%, water hyacinth extract solution 21%, ethanol 10%, propane diols 9% are added, is stirred；
(2) urea 9%, carboxymethyl cellulose 4% are added into step (1), stir to obtain mixed liquor；
(3) above-mentioned mixed liquor is made to the pool-cleaning agent of solid type of spray dryer, the spray drying condition is：Charging
For liquid concentration up to 11 Baume degrees, the Baume degrees is 60 DEG C of Baume degrees measured：190 DEG C of PG-5 type spray dryers inlet temperature,
100 DEG C of outlet temperature, centrifugal head operating pressure 3.0kg/cm2。
The preparation method of above-mentioned Chinese medicine extract is the same as embodiment 1；
The preparation method of the above-mentioned general bacterium MDM nutrient solutions of pineapple is the same as embodiment 1；
The preparation method of above-mentioned water hyacinth extract solution is the same as embodiment 1；
Embodiment 4：
A kind of preparation method of natural environmental-protective type pool-cleaning agent, comprises the following steps:
(1) raw material of following percentage by weight is added into stainless steel first：The general bacterium of Chinese medicine extract 30%, pineapple
MDM nutrient solutions 30%, water hyacinth extract solution 15%, ethanol 6%, propane diols 4%, isopropanol 5% are added, is stirred；
(2) xanthans 7%, urea 2%, carboxymethyl cellulose 1% are added into step (1), stir to obtain mixed liquor；
(3) above-mentioned mixed liquor is made to the pool-cleaning agent of solid type of spray dryer, the spray drying condition is：Charging
For liquid concentration up to 15 Baume degrees, the Baume degrees is 60 DEG C of Baume degrees measured：200 DEG C of PG-5 type spray dryers inlet temperature,
110 DEG C of outlet temperature, centrifugal head operating pressure 1.5kg/cm2。
The preparation method of above-mentioned Chinese medicine extract is the same as embodiment 2；
The preparation method of the above-mentioned general bacterium MDM nutrient solutions of pineapple is the same as embodiment 2；
The preparation method of above-mentioned water hyacinth extract solution is the same as embodiment 2；
Embodiment 5：
A kind of preparation method of natural environmental-protective type pool-cleaning agent, comprises the following steps:
(1) raw material of following percentage by weight is added into stainless steel first：The general bacterium of Chinese medicine extract 25%, pineapple
MDM nutrient solutions 15%, water hyacinth extract solution 25%, ethanol 2%, propane diols 3%, isopropanol 3% are added, is stirred；
(2) xanthans 5%, urea 7%, carboxymethyl cellulose 5% are added into step (1), stir to obtain mixed liquor；
(3) above-mentioned mixed liquor is made to the pool-cleaning agent of solid type of spray dryer, the spray drying condition is：Charging
For liquid concentration up to 16 Baume degrees, the Baume degrees is 60 DEG C of Baume degrees measured：220 DEG C of PG-5 type spray dryers inlet temperature,
100 DEG C of outlet temperature, centrifugal head operating pressure 2kg/cm2。
The preparation method of above-mentioned Chinese medicine extract is the same as embodiment 1；
The preparation method of the above-mentioned general bacterium MDM nutrient solutions of pineapple is the same as embodiment 2；
The preparation method of above-mentioned water hyacinth extract solution is the same as embodiment 1；
Embodiment 6：
A kind of preparation method of natural environmental-protective type pool-cleaning agent, comprises the following steps:
(1) raw material of following percentage by weight is added into stainless steel first：The general bacterium of Chinese medicine extract 28%, pineapple
MDM nutrient solutions 25%, water hyacinth extract solution 18%, propane diols 4%, isopropanol 9% are added, is stirred；
(2) xanthans 10%, urea 6% are added into step (1), stir to obtain mixed liquor；
(3) above-mentioned mixed liquor is made to the pool-cleaning agent of solid type of spray dryer, the spray drying condition is：Charging
For liquid concentration up to 16 Baume degrees, the Baume degrees is 60 DEG C of Baume degrees measured：220 DEG C of PG-5 type spray dryers inlet temperature,
100 DEG C of outlet temperature, centrifugal head operating pressure 2kg/cm2。
The preparation method of above-mentioned Chinese medicine extract is the same as embodiment 2；
The preparation method of the above-mentioned general bacterium MDM nutrient solutions of pineapple is the same as embodiment 1；
The preparation method of above-mentioned water hyacinth extract solution is the same as embodiment 2；
Application examples
Pool-cleaning agent prepared by the embodiment of the present invention 1~6 is spent into chlorine water and is configured to the solution that volume fraction is 10%, is
Pool-cleaning agent dilution.
Culture experiment is carried out in Hefei City, Anhui Province Chaohu Jiang Ka seedling breedings Co., Ltd, and it is equal to choose 7 specifications
Fishpond；Experiment sets 6 test groups (launching pool-cleaning agent made from 1~6 group of the embodiment of the present invention respectively), 1 control group (city
Sell pool-cleaning agent).1 month before pond under fry, remove all weeds debris of fish pond surrounding and establish protection purse seine.Selection fine day, 8
When~12 when the water filling into fishpond, make water surface distance bottom of pond highly be 3 cun, water sampling is standby；Launch pool-cleaning agent respectively again, open
Aerator stirs 10~20min of water body, is distributed medicaments uniformity, does not stay dead angle.Water is taken to count organism in water daily.
Bacterial counting：With the test tube to sterilize, select in pond at representational 3 points in each say, every collection water
Sample 1 is managed, and is diluted respectively with 1/10,1/100,1/1000 dilution factor, is counted after cultivating 24h on ordinary culture medium flat board
Number.The quantity of bacterium in 1ml samples is finally converted into, the results are shown in Table 1.
The collection and counting of zooplankter：
From the 4th day to clean up the pond, every four days collection water samples once.Select in pond at representational 5 points, take at every
Water 200ml, common 1000ml, which are put into settling vessel, to be added 15ml glug liquid and fixes, and is stood 24h, is suctioned out supernatant, leave precipitation and pour into
In 25ml reagent bottles.During counting, reagent bottle is uniformly first shaken into 3min, 0.1ml is suctioned out with the suction pipe of 0.1ml scales, in low power
Mirror is counted under (10 × 8), the results are shown in Table 2.
Table 1 discharges the change of bacterial number in water after pool-cleaning agent
From table 1, the pool-cleaning agent made from the present embodiment 1~6, Most bacterial in water can be killed, and compareed
Group only kills the bacterium of sub-fraction, illustrates that the pool-cleaning agent bactericidal effect of the present invention is preferable.
Table 2 discharges the change of zooplankter quantity in water after pool-cleaning agent
As shown in Table 2, the pool-cleaning agent made from the present embodiment 1~6, the zooplankter in water in after cleaning up the pond the 4th~12 day
Quantity be gradually reduced, and control group is in the 8th~12 day, in water that the quantity of zooplankter is almost unchanged, illustrates the present invention's
Pool-cleaning agent has a great influence to zooplankter in water, plays effect of cleaning up the pond well.
Zooplankter animal includes：Plate shell worm, anxious trip worm, wheel animalcule, chironomus larvas, ship ovum flea, sword flea, Hua Dart fleas etc..
The present invention is described in detail with reference to the foregoing embodiments, it will be understood by those within the art that:
It can still modify to the technical scheme described in foregoing embodiments, or which part technical characteristic is carried out etc.
With replacement；And these modifications or replacement, the essence of appropriate technical solution is departed from various embodiments of the present invention technical scheme
Spirit and scope.
Claims (9)
- A kind of 1. natural environmental-protective type pool-cleaning agent, it is characterised in that：It is made up of the raw material of following percentage by weight：Chinese medicine extract 15 ~40%, the general bacterium MDM nutrient solutions 14~35% of pineapple, water hyacinth extract solution 15~25%, cosolvent 8~19%, thickener 10 ~19%.
- 2. pool-cleaning agent according to claim 1, it is characterised in that：The Chinese medicine extract is the fruit of Rangoon creeper, eucalyptus robusta and soil The taste Chinese medicine extract of schizonepeta 3.
- 3. pool-cleaning agent according to claim 1, it is characterised in that：It is made up of the raw material of following percentage by weight：Chinese medicine carries Take the general bacterium MDM nutrient solutions 25% of liquid 28%, pineapple, water hyacinth extract solution 18%, cosolvent 13%, thickener 16%.
- 4. pool-cleaning agent according to claim 1, it is characterised in that：The preparation method of the Chinese medicine extract, including it is following Step：(1) 10~20 parts of the fruit of Rangoon creeper, 5~16 parts of eucalyptus robusta, 11~25 parts of the taste medicine of chenopodium ambrosiodies 3 are put into pot, added The water of 8~10 times of amounts of medicinal material total amount, after soaking 20~40min, heating boils 0.5~1h, filters, obtains first extract；(2) water of 4~6 times of amounts of medicinal material total amount are added into the dregs of a decoction filtered out, heating boils 0.5~1h, filters, obtains secondary carry Take liquid；(3) first extract and secondary raffinate are mixed, stirred, produce the Chinese medicine extract.
- 5. pool-cleaning agent according to claim 1, it is characterised in that：The preparation method of the general bacterium MDM nutrient solutions of described pineapple For：(1) the general bacterium MDM of pineapple is seeded in 4L~6L YEB fluid nutrient mediums, in 35~38 DEG C, 200rpm concussion and cultivates 20 ~25h, obtains seed liquor；(2) above-mentioned seed liquor is pressed to 1% inoculum concentration, is forwarded in 300L YEB culture mediums, 35~38 DEG C, 200rpm concussion trainings 10~18h is supported, centrifuging and taking supernatant, produces the general bacterium MDM nutrient solutions of the pineapple.
- 6. pool-cleaning agent according to claim 1, it is characterised in that：The preparation method of described water hyacinth extract solution, including Following steps：(1) fetch water after cucurbit herb is cleaned and drain, dried under nature, dry powder is crushed to obtain with pulverizer；(2) dry powder of water hyacinth is taken in container, by 1:4 volume ratio adds petroleum ether, and 1h~2h, mistake are extracted at 50~70 DEG C Filter, filter residue repeat extraction 3 times by above-mentioned volume ratio with petroleum ether, merging filtrate, with Rotary Evaporators rotary evaporation powdery, treated With.
- 7. according to the pool-cleaning agent described in claim any one of 1-6, it is characterised in that：The cosolvent be selected from ethanol, propane diols, One or more in isopropanol.
- 8. according to the pool-cleaning agent described in claim any one of 1-6, it is characterised in that：The thickener is selected from xanthans, carboxylic first One or more in base cellulose, urea.
- 9. the preparation method of the pool-cleaning agent according to claim any one of 1-6, it is characterised in that：Comprise the following steps：(1) fruit of Rangoon creeper, eucalyptus robusta, chenopodium ambrosiodies extract solution, the general bacterium MDM nutrient solutions of pineapple, water hyacinth extract solution are added to first In stainless steel, cosolvent is added, is stirred；(2) thickener is added into step (1), stir to obtain mixed liquor；(3) above-mentioned mixed liquor is made to the pool-cleaning agent of solid type of spray dryer.
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| CN201710509087.9A CN107439616A (en) | 2017-06-28 | 2017-06-28 | A kind of natural environmental-protective type pool-cleaning agent and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111937915A (en) * | 2020-07-07 | 2020-11-17 | 临沂垂闲商贸有限公司 | Aquatic organism inducer and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6174538B1 (en) * | 1991-10-31 | 2001-01-16 | Micro Flo Company | Bait with cucurbitacin |
| CN101416648A (en) * | 2008-11-26 | 2009-04-29 | 黄勤 | Disinfectant using hyacinth as raw material, preparation method and use thereof |
| CN103444806A (en) * | 2013-09-10 | 2013-12-18 | 苏州莲花岛生态农业专业合作社 | Hyacinth plant based nano slow-release oil suspending agent and preparation method thereof |
| CN104531553A (en) * | 2014-11-27 | 2015-04-22 | 安徽农业大学 | Pantoea ananatis Z1 having molluscicidal activity and application thereof |
-
2017
- 2017-06-28 CN CN201710509087.9A patent/CN107439616A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6174538B1 (en) * | 1991-10-31 | 2001-01-16 | Micro Flo Company | Bait with cucurbitacin |
| CN101416648A (en) * | 2008-11-26 | 2009-04-29 | 黄勤 | Disinfectant using hyacinth as raw material, preparation method and use thereof |
| CN103444806A (en) * | 2013-09-10 | 2013-12-18 | 苏州莲花岛生态农业专业合作社 | Hyacinth plant based nano slow-release oil suspending agent and preparation method thereof |
| CN104531553A (en) * | 2014-11-27 | 2015-04-22 | 安徽农业大学 | Pantoea ananatis Z1 having molluscicidal activity and application thereof |
Non-Patent Citations (5)
| Title |
|---|
| 占家智等: "《观赏鱼养殖500问》", 30 September 2003, 金盾出版社 * |
| 姬广闻等: "《新编鱼病综合防治手册》", 30 April 2002, 中原农民出版社 * |
| 肖文渊等: "《水产养殖学专业基础实验实训》", 31 October 2013, 北京理工大学出版社 * |
| 董 原等: ""入侵我国几种外来杂草的化感作用及利用展望"", 《安徽农学通报》 * |
| 郑继方等: "《兽医中药学》", 31 December 2012, 金盾出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111937915A (en) * | 2020-07-07 | 2020-11-17 | 临沂垂闲商贸有限公司 | Aquatic organism inducer and preparation method thereof |
| CN111937915B (en) * | 2020-07-07 | 2022-05-20 | 临沂垂闲商贸有限公司 | Aquatic organism inducer and preparation method thereof |
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Application publication date: 20171208 |