CN107429211A - Stacked thin plate bioreactor - Google Patents

Stacked thin plate bioreactor Download PDF

Info

Publication number
CN107429211A
CN107429211A CN201580000320.XA CN201580000320A CN107429211A CN 107429211 A CN107429211 A CN 107429211A CN 201580000320 A CN201580000320 A CN 201580000320A CN 107429211 A CN107429211 A CN 107429211A
Authority
CN
China
Prior art keywords
thin plate
bioreactor
stacked
cofferdam
culture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201580000320.XA
Other languages
Chinese (zh)
Inventor
胡强
迟庆雷
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sdic Biotechnology Investment Co ltd
Original Assignee
State Development & Investment Corp
Chinese Electronics Engineering Design Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by State Development & Investment Corp, Chinese Electronics Engineering Design Institute filed Critical State Development & Investment Corp
Publication of CN107429211A publication Critical patent/CN107429211A/en
Pending legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00Apparatus for enzymology or microbiology
    • C12M1/16Apparatus for enzymology or microbiology containing, or adapted to contain, solid media
    • C12M1/18Multiple fields or compartments
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02Photobioreactors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/22Transparent or translucent parts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/06Plates; Walls; Drawers; Multilayer plates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M3/00Tissue, human, animal or plant cell, or virus culture apparatus
    • C12M3/04Tissue, human, animal or plant cell, or virus culture apparatus with means providing thin layers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M31/00Means for providing, directing, scattering or concentrating light
    • C12M31/08Means for providing, directing, scattering or concentrating light by conducting or reflecting elements located inside the reactor or in its structure
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12Unicellular algae; Culture media therefor

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Cell Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Immunology (AREA)
  • Botany (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Provide a kind of stacked thin plate bioreactor and the method using the bioreactor culture photosynthetic microorganism, the reactor is included in multiple thin plates of arranged stacked on vertical direction, the multiple thin plate to be spaced a preset distance between adjacent thin plate by support, each thin plate is horizontally extending, with opposite upper and lower surface, and it is provided peripherally with the cofferdam upwardly extended from the upper surface of corresponding thin plate, the upper surface of each thin plate and the inner surface of cofferdam form the space of the nutrient solution for accommodating training objective photosynthetic microorganism, the minimum vertical height of the cofferdam is not more than 10cm.

Description

Stacked thin plate bioreactor Technical field
The present invention relates to photobioreactor fields, and in particular to a kind of stacked thin plate bioreactor.
Background technique
Photosynthetic microorganism is that a kind of microorganism, including microalgae, cyanobacteria to be lived with light for solely or mainly energy source etc. is contained chlorophyll, can carry out photosynthetic microorganism.This quasi-microorganism has important biological utilisation value, especially microalgae, is rich in protein, can be used as aquatic feed or animal and fowl fodder (such as spirulina);It is prior, certain microalgaes can largely synthesize secondary metabolites under given conditions, such as grease, carotenoid, polysaccharide, these substances are often the bioactive substance with high economic value, can be used in the fields such as functional food, food additives, pharmacy, bioenergy.Wherein, microalgae grease is extracted by microalgae large-scale culture, so be converted to liquid fuel, such as biodiesel is considered as solving one of bioenergy production and most important approach of carbon fixation and emission reduction.
Microalgae mass propgation has decades history, and current industrialization microdisk electrode is liquid submersion, i.e., algae is immersed in culture solution and is cultivated as the medium of micro algae growth using mass propgation liquid.Immersion culture mainly includes open culture pond and Closed photobioreactor (photo-bioreactor, PBR) two kinds of forms again.
The advantages of open culture pond is construction and operation, and cost is relatively low, however, liquid surface lower part frustule is weak by luminosity, bottom of pond cell is often difficult to receive abundant illumination, moreover, open pond takes up a large area, also need in culture using powerful agitating device and aerator.In addition, open culture pond is influenced very greatly, vulnerable to the pollution of bacterial body and pest and disease damage, to directly affect the growth and biomass proliferation of algae by external elements.
In contrast to this, closed PBR is usually used translucent material (such as glass, organic glass, plastic film) and the small container of optical path is made.Such as horizontal or vertical glass tube, the existing bioreactor of flat glass flume, illuminating area/volume of its cultivating system is bigger, and cell illumination is more abundant, and photosynthetic microorganism cell culture density is more open, and culture pond is higher, environmental condition controllability is strong, is less susceptible to external environment pollution.But closed PBR be equally also required to circulation agitating device, aerator, And effective culture area of unit space volume is smaller.Moreover, the cost of such PBR usually its ontology is costly, operation expense is high; it is difficult to pilot scale culture; it is unable to reach ideal industrialization target, these are to realize the reason of most direct restraining factors of micro-algae large-scale industry and microalgae product cost are unable to reach popular consumption target.Meanwhile these traditional immersion training methods can not such as be delivered vegetables for the high-efficient culture of some special algae, nostoc and land for building dish due to that must use stirring, aeration, circulation machinery etc..
In addition, Closed photobioreactor is according to glass material, since glass container generallys use technological process for blowing; large-scale, suitable for large-scale production glass container is made, presently, there are very big technical difficulty, even the glass tube of middle-size and small-size test; its cost is also quite high; and during assembling, it will also be by many connection components, such as dedicated rubber connecting sleeve; connector; the breakage of glass tube or connection component, can also often occur in U-bend etc., and maintenance gets up also to have any problem.
It is above-mentioned open, closed since culture solution is ceaselessly being recycled and flowed, once the part of culture solution is contaminated, then fulminant pollution is generated within the scope of whole culture solutions quickly;And solid training method, porous material surface feed flow is given often through the liquid feed device at top, therefore not can avoid the fast propagation of pollution sources equally.
In addition, algae to be attached to the porous material surface that can adsorb and discharge moisture there are also the bioreactor of a kind of solid state rheology, by slowly supplying culture solution to porous material, for adhering to algae growth thereon.However this process for solid culture, the water retention property and algae attachment characteristic of porous material are often largely relied on, if material poor water retention property, needs ceaselessly to supply water, expends energy;And if algae adhesion is poor, algae is easily detached from, then can not make algae normal growth.These porous materials are often light-proof material, can adsorb more luminous energy, and algae is not high to the utilization efficiency of light, and porosity can also allow algae to enter and block duct in it, bring difficulty to subsequent feed flow, harvest, cleaning and disinfection.Moreover, the solid state rheology mode is often not properly suited for the biggish algae of volume, such as deliver vegetables, the culture of nostoc and land for building dish.
In conclusion existing bioreactor substantially exists the following technical problem: 1., the land occupation of open raceway pond it is big, culture density is low, and culture solution circulating consumption is high, vulnerable to outside contamination;2., closed light transmission container cost it is high, especially glass container is limited to the particularity of glass machining technique, can not one-pass molding, manufacturing cost, installation and maintenance cost are all very high, and it is low to equally exist cell density, space utilization degree is insufficient, the high problem of energy consumption.3., either open raceway pond or closed light transmission container, require consumption mass propgation liquid and energy, culture solution concentration is low when harvest, need to by cumbersome separation, filtering and the technological deficiency for being dried program, can not reduce microalgae product at This.4. solid state rheology bioreactor, very big for the dependence of material itself, light utilization ratio is low, applicability also compares and is limited to, and liquid feed device is a kind of energy-consuming device, therefore can not further reduce toxigenic capacity.5. existing bioreactor is unfavorable for being effectively isolated and controlling for pollution sources.
Thus, the present inventor wants to provide that a kind of cost is cheap, the effective culture area of unit space volume is bigger, optical energy utilization efficiency is high, low energy consumption for culture, applicability is wide, harvest program simplification and the bioreactor suitable for the extensive industrialization culture of algae, so as to the whole production cost for reducing microalgae.
Summary of the invention
It is high that the object of the present invention is to provide a kind of yields, and less energy consumption, applicability be wide, suitable for the bioreactor of pilot scale culture.
For this purpose, provide a kind of stacked thin plate bioreactor, multiple thin plates including the arranged stacked on vertical direction, the multiple thin plate is supported so that being spaced a preset distance between adjacent thin plate, each thin plate is horizontally extending, with opposite upper and lower surfaces, and it is provided peripherally with the cofferdam upwardly extended from the upper surface of corresponding thin plate, the upper surface of each thin plate and the inner surface of cofferdam form the space of the culture solution for accommodating training objective photosynthetic microorganism (hereinafter referred to as " photo-biological "), wherein, the vertical height of the minimum of the cofferdam is not more than 10cm.
By features described above, it can make stacked thin plate bioreactor of the invention when cultivating microalgae, the thickness of culture solution is no more than 10cm, thus can without stirring, and the photosynthetic microorganism in culture solution bottom still can receive sufficient illumination.
According to an embodiments possible, multiple support columns are set, the support column is used to support thin plate above between adjacent thin plate.
According to an embodiments possible, the cofferdam is integrally formed with corresponding thin plate, or corresponding thin plate is attached to after independently forming.
According to a feasible embodiment, the support column is integrally formed with corresponding thin plate, or the lower surface of corresponding thin plate is attached to after independently forming.
According to an embodiments possible, the cofferdam relative to corresponding thin plate upper surface at the inclination angle for being greater than or equal to 90 degree.
According to an embodiments possible, the cofferdam is higher by the upper surface 1mm-10cm of corresponding thin plate, preferably 1mm-5mm, 10mm-30mm or 30mm-100mm.
According to an embodiments possible, the cofferdam is made of the material identical or different with thin plate and/or the support column is made of the material identical or different with thin plate.
According to an embodiments possible, the support column is the form of column or pipe with round, rectangle or square cross section.
According to an embodiments possible, the thin plate is made of the high plastics of the glass or light transmittance of light transmission.
According to an embodiments possible, the thin plate is made of one of materials described below: glass, GPPS, transparent ABS, AS (styrene-acrylonitrile), PVC, PMMA (polymethyl methacrylate), PC (polycarbonate), PS (polystyrene).
According to an embodiments possible, the thin plate is made of plate glass, and the cofferdam is attached to the adhesive tape of thin plate, preferably cellophane adhesive tape, i.e., the accommodation space of a culture solution is formed in one circle cellophane adhesive tape of the surrounding bonding of the plate glass.
According to an embodiments possible, the thin plate is made of organic material, and the cofferdam and thin plate are integrally formed.
According to an embodiments possible, the spacing between each thin plate is identical or different.
According to an embodiments possible, the size of each thin plate is sequentially increased from top to bottom, and at least side of bioreactor is made to show inclined plane shape.The side on the inclined-plane is equipped with light source, by inclined-plane, can make to enter more light between two layers of thin plate.
According to an embodiments possible, the specification of the thin plate is length 10m × width 1m, and the spacing between adjacent sheet is 0.04m.
According to an embodiments possible, the hole that light can penetrate is formed on the support column.
The present invention also provides a kind of methods cultivated using above-mentioned stacked thin plate bioreactor photosynthetic microorganism, wherein, the method is the upper surface for making culture solution be housed inside each thin plate with thin layer and static state in cultivation cycle, and wherein the thickness of culture solution is not more than 10cm.
Stacked thin plate bioreactor according to the present invention, the culture plate for carrying out photo-biological culture is plate, and processing and manufacturing is simple, at low cost, and can accomplish that the culture area of single culture plate is larger, and thickness can also be relatively thin, and transparency can be higher.Multiple plates are stacked vertically, keep structure integrated, and effective culture area of unit space occupied maximizes, and have significant specific surface area advantage.Since each thin plate is transparency material, so light-receiving area is big, light utilization ratio is very high.Using bioreactor of the invention, the algae solution on each layer thin plate is made thinner, and is directly contacted with environment with one surface, thus can sufficiently with CO in air2While contact and absorption, also sufficiently outside reception Illumination, makes the photosynthetic microorganism of the culture solution bottom also can receive light source, and reduction is blocked;The integrated fixation for being conducive to reactor of structure and raising space efficiency utilization.During cultivating microalgae with the present invention, a large amount of water body is not needed, without Shu Song circulation machinery or stirring operation, saves conveying, circulation and stirring energy consumption, and illumination can be obtained well, effectively controls the explosive diffusion of local pollution sources;In harvest, since Culture liquid measure is few, cultivated photo-biological and culture solution separating difficulty is reduced, harvest cycle is shortened, significant economic benefit can be obtained.Bioreactor of the invention has started a kind of thin layer, static training method, is a kind of completely new training mode between immersion culture and solid state culture, more can be applied to the cultivation of volume and the multifarious various algae of form.
Detailed description of the invention
The embodiment provided by reference to attached drawing is described in detail above and other aspect, advantage and feature of the invention.But it will be understood to one skilled in the art that embodiment shown in figure is used merely to illustrate the principle of the present invention, and it is not meant to limit the scope of the invention.Protection scope of the present invention is only defined by the claims.Wherein:
Fig. 1 is the perspective view of stacked thin plate bioreactor according to the preferred embodiment of the invention;
Fig. 2 is the front view of Fig. 1;And
Fig. 3 is the cross-sectional view of the A-A along Fig. 1.
Fig. 4 is the perspective view for the horizontal tube pipeline bioreactor being contrasted with stacked thin plate bioreactor according to the present invention.
Fig. 5 is in 200m2Occupied area in the space layout schematic diagram of photo-biological culture is carried out using the reactor in Fig. 4.
Fig. 6 is in 200m2Occupied area in using Fig. 1 stacked thin plate bioreactor carry out photo-biological culture space layout schematic diagram.
Specific embodiment
Generally, bioreactor provided by the invention is a kind of stacked thin plate bioreactor, it includes being laminated on vertical direction and in multiple thin plates of preset space length arrangement, the multiple thin plate is horizontally extending, each thin plate has the upper surface and opposite lower surface for placing the culture solution of training objective photo-biological, and the periphery of each thin plate is equipped with the cofferdam upwardly extended from the upper surface for favouring corresponding thin plate that corresponding thin plate stretches out.
Fig. 1 to 3 shows stacked thin plate bioreactor 100 according to the preferred embodiment of the invention.
It can be seen from the figure that stacked thin plate bioreactor 100 includes multiple thin plates 20 of the arranged stacked on vertical direction and multiple support columns 40 between adjacent thin plate.Each thin plate 20 is horizontally extending, and has the upper surface 22 and opposite lower surface 24 for placing the culture solution of training objective photo-biological.The periphery of each thin plate 20 is equipped with the cofferdam 26 upwardly extended from the upper surface 22 for favouring corresponding thin plate 20 that corresponding thin plate 20 stretches out.
Each thin plate 20 forms the culture plate of bioreactor, that is, the upper surface 22 of each thin plate 20, the inner surface of the cofferdam 26 of thin plate 20 limit the space 30 for containing culture solution.
The transparent plate made of glass material or organic material can be used in the thin plate 20 for forming bioreactor.For tubulose, channel-shaped or can-like bioreactor, the bioreactor processing and manufacturing according to the present invention being made of plate is simple, at low cost, and can accomplish that the culture area of single culture plate is larger, thickness can also be relatively thin, and transparency can be higher.
Each thin plate 20 arranged stacked in the vertical direction, keeps structure integrated, and effective culture area of unit space occupied maximizes.
Since the material of each thin plate 20 is the high material of transparency, in the Initial stage of culture of algae, since bottle-green biomembrane has not yet been formed in the algae on each thin plate 20, therefore on the culture liquid level that external light can reach adjacent lower layer's thin plate through each 20 upper surface of layer thin plate, equally can also be from the culture liquid level that the lower surface of each layer thin plate 20 reaches upper layer thin plate, light efficiency is higher.Light source is irradiated to the photo-biological cell cultivated on the upper surface 22 of following thin plate 20 from the frustule above the upper surface direct irradiation of thin plate 20 and through thin plate 20, so light-receiving area is big, light utilization ratio is very high.
Using the bioreactor of this structure, the water body used is few, so the culture of photo-biological saves energy consumption without stirring.In addition, since water is few, when harvest, can reduce process and thus reduce harvest cost.Thus, the bioreactor culture photo-biological using this structure is static gas wave refrigerator, is not necessarily to provide conveying or stirring power in incubation, and can obtain illumination well.
According to the present invention, the cofferdam 26 of each thin plate 20 can be integrally formed with thin plate 20, and thin plate 20 is attached to after can also independently forming.
When thin plate 20 is made of glass material, cofferdam 26 can be the circle adhesive tape for being adhered to 20 periphery of thin plate, preferably clear adhesive tape.For the thin plate 20 of organic material, cofferdam 26 can be integrally formed with thin plate 20.
The material for forming thin plate 20 can be glass or the high plastics of light transmittance, such as GPPS, transparent ABS, AS (styrene-acrylonitrile), PVC, PMMA (polymethyl methacrylate), PC (polycarbonate), PS (polystyrene) etc..The material for forming cofferdam 26 can be material identical with thin plate 20, can also use other transparent materials, such as adhesive tape, glass cement.
The upper surface 22 of cofferdam 26 and thin plate 20 is at the angle for being greater than or equal to 90 degree, in order to which the photo-biological cultivated on upper surface 22 is gone out with high pressure water/air-blowing to outside thin plate 20, to realize the harvest of photo-biological;Preferably the upper surface 22 of cofferdam 26 and thin plate 20 at the angle greater than 90 degree and forms fillet between the two junction, prevents from forming harvest dead angle or cleaning dead angle.Optionally, it is 1mm-10cm, preferably 1mm-5mm that the cofferdam 26, which is higher by the distance of the upper surface 22 of corresponding thin plate 20,.
Spacing between each thin plate 20 is required according to light source arrangement, the culture of photo-biological and harvest to determine.
Shape, material, quantity, spacing and the arrangement of support column 40 can not limit, and support column 40 is only needed to have some strength, the culture solution being supported on the thin plate 20 and its upper surface 22 of stacked on top enough.Preferably, support column 40 uses transparent material, such as glass, acrylic, PC material.Support column 40 can have any suitable shape, including but not limited to cylinder, tubular, rectangular, strip etc..
In the illustrated embodiment, support column 40 is arranged in banks.Additionally optionally, the hole for enabling light to penetrate is provided on support column 40.
For sheets of glass 20, support column 40 can use glass column, be also possible to non-glass column.Support column 40 can all be separated with above and below thin plate 20, can also be adhered to the lower surface 24 of thin plate 20 above.
For the thin plate 20 made of organic transparent material, support column 40 can be integrally formed with thin plate 20.
Using stacked thin plate bioreactor 100 according to the present invention, the technical purpose for largely increasing specific surface area is realized.However, this may cause the problem of original water seldom culture solution rapid evaporation, thus, the culture solution drop instillator 50 that culture solution can be supplemented to each thin plate 20 can be further arranged in stacked thin plate bioreactor of the invention, culture solution drop instillator 50 diagrammatically illustrates in Fig. 3, and optionally, the leve monitor (not shown) of the accurate liquid level of each thin plate 20 of configurable measurement.In addition light source 60 is shown in FIG. 3.
Additionally optionally, stacked thin plate bioreactor of the invention can further include the light-transmissive film cover for being encapsulated stacked thin plate bioreactor, provide steady temperature, the atmosphere of air humidity for its internal stacked thin plate bioreactor.This can reduce the evaporation of moisture, to photo-biological Most appropriate growth conditions is created in culture, so can no longer need to the culture solution that replenishes the supply in the cultivation cycle of photosynthetic microorganism.
Other than the bioreactor of above-described embodiment, each thin plate 20 can be also individually positioned on the bracket with several cross bars, and the gravity of each thin plate 20 and culture solution is dispersed by the bracket;It equally can achieve technical effect identical with previous embodiment.
Bioreactor according to the present invention does not need a large amount of water body, therefore in links such as harvest, concentration, drying, water process; a large amount of workload and energy consumption can be reduced; to reduce overall cost when harvest, significant economic benefit yield height is obtained, pilot scale culture is suitable for.
The illuminating area of bioreactor is one of the most key factor for evaluating bioreactor culture efficiency.In the following, identical nutrient solution volume is directed to, compared with so that stacked thin plate bioreactor of the invention is received the area of illumination with traditional both horizontal tube bioreactors, to illustrate the advantage of stacked thin plate bioreactor of the invention.
Receive the comparison of illuminating area:
Traditional horizontal tube bioreactor: 1L volume algae solution occupies the glass pipe that diameter is 50mm, is computed its light receiving surface product are as follows: 0.04m2
Stacked thin plate bioreactor of the invention: 1L volume algae solution, liquid level thickness 3mm, double-side photic area are as follows: 0.66m2.As it can be seen that the algae solution of same volume, which receives illuminating area, improves about 16.67 times.
In order to further explain the advantage of bioreactor of the present invention, illustrate the advantage of bioreactor according to the present invention by way of example again below.
What is be contrasted with bioreactor according to the present invention is the horizontal tube pipeline bioreactor 200 provided in Fig. 4 with perspective view, and arrow indicates that culture solution flows into and out reactor 200.
With 200m2Occupied area for (Fig. 5-6 arithemetic unit is mm), as shown in figure 5, because to arrange with the matching used pump 210 and aeration tank 220 of reactor 200, can only arrange the horizontal tube pipeline bioreactor that 4 sets of such as Fig. 4 show.Assuming that the height of every set horizontal tube pipeline bioreactor is 2.5m, total volume of culture amount 4000L, in 10 days incubation times, with yield of biomass 3g/L calculating, dry matter 12kg is harvested altogether.
Equally in 200m2Occupied area in, as shown in Figure 6 use stacked plate TLC bioreactor according to the present invention, additional auxiliary facility as shown in Figure 5 is not needed, so 50 reactors 100 can be arranged.Assuming that the height of reactor 100 is similarly 2.5m, 20 specification of thin plate made of plate glass is 2.0 × 0.8m, and the spacing between adjacent sheet 20 is 10mm, each reactor 100 can be laminated 250 layers, each bioreactor culture area 400m2, then total culture area of 50 reactors 100 is 400 × 50=20000m2.Conservative estimation every square meter culture biological quality 1g/m daily2/ d, same cultivation cycle 10 days, then yield of biomass are as follows: 10d × 20000m2×1g/m2/ d=200kg.
From above-mentioned comparison, it is about 16.67 times for using the obtained yield of biomass of horizontal tube pipeline bioreactor using the stacked obtained yield of biomass of plate TLC bioreactor according to the present invention in the case where identical occupied area and space occupied.
With needing a large amount of water bodys, need a large amount of power consumptions come compared with conveying culture solution but also needing traditional duct type bioreactor of agitating device, stacked thin plate bioreactor according to the present invention is static gas wave refrigerator, a large amount of water body is not needed, without conveying or stirring operation in incubation, conveying and stirring power are saved, and illumination can be obtained well.In addition, culture solution concentration is significantly larger than traditional duct type bioreactor, therefore in links such as harvest, concentration, drying, water process, so that it may reduce a large amount of photo-biological-culture solution separation workload and energy consumption, to reduce overall cost when harvest, significant economic benefit is obtained.
Since the present invention started for the first time in incubation using static (not having to stirring), (Culture liquid measure is few for thin layer, light availability is high) training method, photosynthetic microorganism is placed in suitable Culture liquid measure and is cultivated, therefore it is highly suitable for the culture of volume and the biggish unicellular alga of form, including deliver vegetables, nostoc and land for building dish etc..

Claims (17)

  1. A kind of stacked thin plate bioreactor, it is characterized in that, the stacked thin plate bioreactor includes multiple thin plates of the arranged stacked on vertical direction, the multiple thin plate is supported so that being spaced a preset distance between adjacent thin plate, each thin plate is horizontally extending, with opposite upper and lower surfaces, and it is provided peripherally with the cofferdam upwardly extended from the upper surface of corresponding thin plate, the upper surface of each thin plate and the inner surface of cofferdam form the space for accommodating the culture solution of training objective photosynthetic microorganism, wherein, the minimum constructive height of the cofferdam is not more than 10cm.
  2. Stacked thin plate bioreactor according to claim 1, characterized in that multiple support columns are set between adjacent thin plate, and the support column is used to support thin plate above.
  3. Stacked thin plate bioreactor according to claim 2, it is characterized in that, the cofferdam is integrally formed with corresponding thin plate, or corresponding thin plate is attached to after independently forming, and/or support column is integrally formed with corresponding thin plate, or the lower surface of corresponding thin plate is attached to after independently forming.
  4. Stacked thin plate bioreactor according to claim 2, characterized in that the cofferdam relative to corresponding thin plate upper surface at being greater than or equal to 90 degree of inclination angle.
  5. Stacked thin plate bioreactor according to claim 2, characterized in that the cofferdam is higher by the upper surface 1mm-10cm of corresponding thin plate.
  6. Stacked thin plate bioreactor according to claim 5, characterized in that the cofferdam is higher by upper surface 1-5mm, 10mm-30mm or 30mm-100mm of corresponding thin plate.
  7. Stacked thin plate bioreactor according to claim 1, characterized in that the cofferdam is made of the material identical or different with thin plate and/or the support column is made of the material identical or different with thin plate.
  8. Any stacked thin plate bioreactor, feature in -7 according to claim 1 It is that the support column is the form of column or pipe with round, rectangle or square cross section.
  9. Any stacked thin plate bioreactor in -8 according to claim 1, characterized in that the thin plate is made of the high plastics of the glass or light transmittance of light transmission.
  10. Any stacked thin plate bioreactor in -9 according to claim 1, it is characterized in that, the thin plate is made of one of materials described below: glass, GPPS, transparent ABS, AS (styrene-acrylonitrile), PVC, PMMA (polymethyl methacrylate), PC (polycarbonate), PS (polystyrene).
  11. Stacked thin plate bioreactor according to claim 1, characterized in that the thin plate is made of plate glass, and the cofferdam is attached to the adhesive tape of thin plate, preferably cellophane adhesive tape.
  12. Stacked thin plate bioreactor according to claim 1, characterized in that the thin plate is made of organic material, and the cofferdam and thin plate are integrally formed.
  13. Any stacked thin plate bioreactor in -12 according to claim 1, characterized in that the spacing between each thin plate is identical or different.
  14. Any stacked thin plate bioreactor in -13 according to claim 1, characterized in that the size of each thin plate is sequentially increased from top to bottom, and at least side of bioreactor is made to show inclined plane shape.
  15. Any stacked thin plate bioreactor in -12 according to claim 1, characterized in that the specification of the thin plate is length 10m × width 1m, and the spacing between adjacent sheet is 0.04m.
  16. Any stacked thin plate bioreactor in -15 according to claim 1, characterized in that the hole that light can penetrate is formed on the support column.
  17. A method of photosynthetic microorganism is cultivated using the stacked thin plate bioreactor any according to claim 1-16, wherein, the method is the upper surface for making culture solution be housed inside each thin plate with thin layer and static state in cultivation cycle, and wherein the thickness of culture solution is not more than 10cm.
CN201580000320.XA 2015-08-14 2015-08-14 Stacked thin plate bioreactor Pending CN107429211A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2015/086982 WO2017028018A1 (en) 2015-08-14 2015-08-14 Stacked sheet photobioreactor

Publications (1)

Publication Number Publication Date
CN107429211A true CN107429211A (en) 2017-12-01

Family

ID=58050452

Family Applications (2)

Application Number Title Priority Date Filing Date
CN201580000320.XA Pending CN107429211A (en) 2015-08-14 2015-08-14 Stacked thin plate bioreactor
CN201610187268.XA Pending CN106467902A (en) 2015-08-14 2016-03-29 A kind of cultural method of photosynthetic microorganism and bioreactor

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN201610187268.XA Pending CN106467902A (en) 2015-08-14 2016-03-29 A kind of cultural method of photosynthetic microorganism and bioreactor

Country Status (2)

Country Link
CN (2) CN107429211A (en)
WO (1) WO2017028018A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114940941A (en) * 2022-07-04 2022-08-26 珠海元育生物科技有限公司 Three-dimensional continuous flow type microalgae photobioreactor

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106916724B (en) * 2015-12-28 2019-11-05 国投生物科技投资有限公司 A kind of scenedesmus cultural method and culture apparatus
CN106916749B (en) * 2015-12-28 2021-03-09 国投生物科技投资有限公司 Nostoc sphaeroids kutz culture method and device
CN113136342B (en) * 2020-01-19 2023-05-05 中国石油化工股份有限公司 Photo bioreactor, photosynthetic microorganism culture method and application thereof

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008283937A (en) * 2007-05-21 2008-11-27 Dialight Japan Co Ltd Photobioreactor
EP2039753A1 (en) * 2007-09-24 2009-03-25 Proviron Holding Bioreactor
CN101942388A (en) * 2009-07-06 2011-01-12 新奥科技发展有限公司 Optical bioreactor
CN101962615A (en) * 2009-07-22 2011-02-02 贝克顿·迪金森公司 Multilayer tissue's culture vessel
CN101978041A (en) * 2008-01-25 2011-02-16 康宁股份有限公司 Limited access multi-layer cell culture system
CN102224236A (en) * 2008-11-12 2011-10-19 拉曼大学教育基金会 A photobioreactor
CN102296022A (en) * 2010-06-25 2011-12-28 新奥科技发展有限公司 Optical bioreactor
CN102482634A (en) * 2009-09-15 2012-05-30 拜尔材料科学有限公司 Photobioreactor for algae growth

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2836218A1 (en) * 2013-12-13 2015-06-13 Soheyl S. M. Mottahedeh Multilevel photobioreactor
CN105296338A (en) * 2015-11-16 2016-02-03 河海大学 Multilayer photobioreactor of algae microbes

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008283937A (en) * 2007-05-21 2008-11-27 Dialight Japan Co Ltd Photobioreactor
EP2039753A1 (en) * 2007-09-24 2009-03-25 Proviron Holding Bioreactor
CN101978041A (en) * 2008-01-25 2011-02-16 康宁股份有限公司 Limited access multi-layer cell culture system
CN102224236A (en) * 2008-11-12 2011-10-19 拉曼大学教育基金会 A photobioreactor
CN101942388A (en) * 2009-07-06 2011-01-12 新奥科技发展有限公司 Optical bioreactor
CN101962615A (en) * 2009-07-22 2011-02-02 贝克顿·迪金森公司 Multilayer tissue's culture vessel
CN102482634A (en) * 2009-09-15 2012-05-30 拜尔材料科学有限公司 Photobioreactor for algae growth
CN102296022A (en) * 2010-06-25 2011-12-28 新奥科技发展有限公司 Optical bioreactor

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
NAVID R. MOHEIMANI ET AL.: "Coccolithophorid Algae Culture in Closed Photobioreactors", 《BIOTECHNOLOGY AND BIOENGINEERING》 *
刘娟妮 等: "微藻培养中光生物反应器的研究进展", 《食品科学》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114940941A (en) * 2022-07-04 2022-08-26 珠海元育生物科技有限公司 Three-dimensional continuous flow type microalgae photobioreactor
CN114940941B (en) * 2022-07-04 2023-03-07 珠海元育生物科技有限公司 Three-dimensional continuous flow type microalgae photobioreactor

Also Published As

Publication number Publication date
CN106467902A (en) 2017-03-01
WO2017028018A1 (en) 2017-02-23

Similar Documents

Publication Publication Date Title
Posten Design principles of photo‐bioreactors for cultivation of microalgae
EP3673728A1 (en) A microalgae-based system for producing products and a process making use thereof
US9260685B2 (en) System and plant for cultivation of aquatic organisms
CN101709264B (en) Optical bioreactor
US20070155006A1 (en) Photobioreactor
EP2667963B1 (en) A fluid agitator device for facilitating development of algae or micro-algae in trays or photobioreactors
CN101914431B (en) Device and method for cultivating microalgae by utilizing all plastic modular photobioreactor system
CN107429211A (en) Stacked thin plate bioreactor
US20130205450A1 (en) Cultivation of photosynthetic organisms
US20120115217A1 (en) Device and method for photosynthetic culture
KR101556771B1 (en) Enclosed photobioreactor for incubating microalgae
CN201424476Y (en) Photobioreactor
KR101646246B1 (en) Photo-bioreactor for attached algae
CN107460129A (en) The industrialization microalgae culture method that biomass separates with nutrient solution
CN106467888B (en) Grated plate type bioreactor
KR101608214B1 (en) Photobioreactor capable of controlling media temperature
CN204644342U (en) For cultivating the microporous culture plate of photosynthetic microorganism
CN106916723B (en) A kind of haematococcus pluvialis cultural method and culture apparatus
CN107988053A (en) A kind of microalgae culture system for realizing Immobilized culture and waste water advanced purifying
KR20230016138A (en) Building detachable algae culturing device
KR101609714B1 (en) Raceway type photosynthetic biomass production apparatus
US20140127766A1 (en) System for obtaining biomass
KR101415553B1 (en) Device for culturing micro algae
KR102456255B1 (en) Convergence building structure for agriculture and fisheries having photobioreactor for cultivation of microalgae
WO2011007250A2 (en) Industrial photobioreactor and structure of the same, of low cost and with high productive yield for occupied surface site

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
CB02 Change of applicant information
CB02 Change of applicant information

Address after: 100034 -6 international investment building, 6 Fuchengmen North Street, Xicheng District, Beijing.

Applicant after: National Development Investment Group Co.,Ltd.

Applicant after: CHINA ELECTRONICS ENGINEERING DESIGN INSTITUE

Address before: 100034 -6 international investment building, 6 Fuchengmen North Street, Xicheng District, Beijing.

Applicant before: STATE DEVELOPMENT AND INVESTMENT Corp.

Applicant before: CHINA ELECTRONICS ENGINEERING DESIGN INSTITUE

TA01 Transfer of patent application right
TA01 Transfer of patent application right

Effective date of registration: 20181126

Address after: 100034 147 Xizhimen South Street, Xicheng District, Beijing

Applicant after: SDIC BIOTECHNOLOGY INVESTMENT Co.,Ltd.

Address before: 100034 -6 international investment building, 6 Fuchengmen North Street, Xicheng District, Beijing.

Applicant before: National Development Investment Group Co.,Ltd.

Applicant before: CHINA ELECTRONICS ENGINEERING DESIGN INSTITUE

RJ01 Rejection of invention patent application after publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20171201