CN114940941B - Three-dimensional continuous flow type microalgae photobioreactor - Google Patents
Three-dimensional continuous flow type microalgae photobioreactor Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/56—Floating elements
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/58—Reaction vessels connected in series or in parallel
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M31/00—Means for providing, directing, scattering or concentrating light
- C12M31/02—Means for providing, directing, scattering or concentrating light located outside the reactor
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/06—Means for regulation, monitoring, measurement or control, e.g. flow regulation of illumination
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/89—Algae ; Processes using algae
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/59—Biological synthesis; Biological purification
Abstract
The invention provides a three-dimensional continuous flow type microalgae photobioreactor which comprises a bracket and more than two light reaction units which are fixed on the bracket and are sequentially stacked along the vertical direction; each photoreaction unit is provided with a transparent snakelike flow channel and a light guide plate which are arranged in the horizontal direction, the light guide plate is positioned under the transparent snakelike flow channel, at least one thickness surface of the light guide plate is attached with an LED light bar, the upper surface of the light guide plate is used as a light emergent surface, and the lower surface and other thickness surfaces of the light guide plate are attached with a reflective film; in the adjacent photoreaction units, the outlet of the upper transparent snakelike flow passage is communicated with the inlet of the lower transparent snakelike flow passage through a connecting pipe; all be provided with in each transparent snakelike runner and float formula light intensity detection module, float formula light intensity detection module and pass through flexible rope and snakelike runner wall connection. Greatly improves the yield of microalgae biomass in unit occupied area.
Description
Technical Field
The invention relates to the technical field of microalgae culture, in particular to a three-dimensional continuous flow type microalgae photobioreactor.
Background
In recent years, the microalgae photosynthetic carbon fixation and energy resource utilization technology has the effect of CO emission reduction 2 The preparation of biofuel, the synthesis of high-value bioactive substances and the like become heat of domestic and foreign researchAnd (4) point. Microalgae cells are considered to be an ideal CD-ROM cell factory, and carbon dioxide and water can be efficiently converted into carbohydrates through photosynthesis under the driving of light energy. Microalgae biomass is rich in pigment, protein, oil and the like, and can be used for preparing diversified algae-based chemicals such as aquaculture baits, feeds, biodiesel, cosmetics, medicines and the like.
Photoautotrophic growth of microalgae is usually carried out in a photobioreactor, and an efficient photobioreactor is the key to obtaining large quantities of microalgae biomass. According to the different movement states of the microalgae cells in the reactor, the existing photobioreactor can be divided into a suspension type photobioreactor and an immobilized biofilm type photobioreactor, wherein the suspension type microalgae photobioreactor is the most important microalgae photoautotrophic culture device at present. The light energy is an energy source for the photoautotrophic growth of the microalgae, in order to improve the performance of the microalgae photobioreactor, it is ensured that as many microalgae cells in the reactor as possible can receive sufficient light, however, the light energy in the reactor is not uniformly distributed due to the severe light attenuation phenomenon in the suspension culture system, and the performance of the reactor is limited. On the other hand, the current photobioreactor suitable for microalgae cultivation has the disadvantage of large floor space, resulting in lower microalgae biomass yield per floor space.
Aiming at the defects of the research, the invention provides an intelligent three-dimensional continuous flow type microalgae photobioreactor based on a light guide plate from the viewpoints of optimizing the light energy distribution in the reactor and improving the yield of microalgae biomass in unit floor area, so as to realize the efficient continuous production of microalgae biomass.
Disclosure of Invention
Technical problem to be solved
In view of the problems in the art described above, the present invention is at least partially addressed. Therefore, the invention aims to provide a three-dimensional continuous flow type microalgae photobioreactor, which greatly improves the microalgae biomass yield in unit floor area.
(II) technical scheme
In order to achieve the purpose, the invention adopts the main technical scheme that:
the invention provides a three-dimensional continuous flow type microalgae photobioreactor which comprises a bracket and more than two light reaction units which are fixed on the bracket and are sequentially stacked in the vertical direction;
each photoreaction unit is provided with a transparent snakelike flow channel and a light guide plate which are arranged in the horizontal direction, the light guide plate is positioned under the transparent snakelike flow channel, at least one thickness surface of the light guide plate is attached with an LED light bar, the upper surface of the light guide plate is used as a light emergent surface, and the lower surface and other thickness surfaces of the light guide plate are attached with a reflective film; in the adjacent photoreaction units, the outlet of the upper transparent snakelike flow channel is communicated with the inlet of the lower transparent snakelike flow channel through a connecting pipe;
all be provided with floating formula light intensity detection module in each transparent snakelike runner, floating formula light intensity detection module passes through flexible rope and snakelike runner wall connection, lets in little algae cell suspension in the transparent snakelike runner after, floats formula light intensity detection module and floats on little algae cell suspension surface to the light intensity sensing probe of floating formula light intensity detection module hugs closely little algae cell suspension surface.
Optionally, the photoreaction unit comprises a box body with an open top or a closed top, the box body is fixedly mounted on the photoreaction unit mounting position of the support, a plurality of partition plates are arranged in the cavity of the box body, the cavity of the box body is shaped into a transparent serpentine channel with a unique flow path by the plurality of partition plates, and a bottom plate of the box body is used as a light guide plate or is tightly attached to the upper surface of the light guide plate; or the light reaction unit comprises a transparent serpentine pipeline, a transparent serpentine flow channel is formed in the transparent serpentine pipeline, and a light guide plate is arranged below the transparent serpentine pipeline.
Optionally, the photobioreactor further comprises a control unit, the floating light intensity detection module is in communication connection with the control unit, and the control unit is electrically connected with the LED light bars of each photoreaction unit.
Optionally, each thickness surface of the light guide plate is attached with an LED light bar, the upper surface of the light guide plate is used as a light emergent surface, and the lower surface of the light guide plate is attached with a reflective film.
Optionally, a pH and inorganic salt concentration monitoring module is arranged in each transparent serpentine flow channel, and an aerator and a concentrated culture medium filling unit are integrated on the pH and inorganic salt concentration monitoring module.
Optionally, the holder has a connection post for connecting adjacent mounting sites of the photoreaction unit, the height of the connection post being adjustably set.
Optionally, the inside of the light guide plate is doped with nano-scale light scattering particles.
Optionally, the connecting pipes between two adjacent light reaction units in the height direction each include a main pipe and a plurality of branch pipes; in two adjacent photoreaction units, the export of the transparent snakelike runner of upper photoreaction unit is a plurality of apopores that arrange in proper order along the direction of height, and every apopore all communicates through the first end of a pipeline and trunk line, and the second end of trunk line and the import intercommunication of the transparent snakelike runner of lower floor photoreaction unit all are provided with the valve on every pipeline.
Optionally, the photobioreactor further comprises a control unit, the valve is a solenoid valve, and the solenoid valve is electrically connected with the control unit.
(III) advantageous effects
The invention has the beneficial effects that:
1. more than two photoreaction units are arranged in an up-down direction and are mutually overlapped, so that the floor area of the photobioreactor is reduced, and the microalgae biomass yield under the unit floor area is improved.
2. In each photoreaction unit, through the attached LED lamp strip of at least one thickness face at the light guide plate, regard the upper surface of light guide plate as the light emergent face, all attached reflective membrane with other faces of light guide plate, the emergent light of LED lamp strip gets into the light guide plate after inside from the side end face of light guide plate, can follow the even outgoing of upper surface of light guide plate, guarantee to be located the transparent snakelike runner of light guide plate top in the light energy distribution even, and then be favorable to all little algae cell homoenergetic in the reactor to receive sufficient illumination, provide the basis for little algae cell's accurate growth regulation in each photoreaction unit simultaneously.
3. Along with the progress of the culture process, the concentration of microalgae cells in the reactor is increased, a floating type light intensity detection module flexibly connected with a flow channel wall is arranged in each transparent snake-shaped flow channel, the floating type light intensity detection module floats on the surface of a microalgae cell suspension liquid under the action of buoyancy and is pulled by a flexible lead fixed on the bottom surface of the snake-shaped flow channel, the flexible lead can ensure that the floating type light intensity detection module is not taken away by the flowing microalgae cell suspension liquid and can also ensure that the floating type light intensity detection module always floats on the surface of the microalgae cell suspension liquid, the floating type light intensity detection module can detect the illumination intensity of a light guide plate on the surface of the suspension liquid finally after emergent light passes through the suspension liquid, the growth environment of the microalgae cells of the light reaction unit is adjusted in real time based on the measured illumination intensity value, the light intensity measured by the floating type light intensity detection module is equal to or slightly higher than the light compensation point of microalgae species, further, all the microalgae cells in the reaction unit can be ensured to receive illumination higher than the light compensation point, the amount of photosynthetic synthesis organic matters consumed by the respiration of the microalgae cells in the reactor, the reactor is more than the amount of the microalgae biomass accumulated in the reactor, and the performance of the microalgae biomass in the reactor is greatly improved.
Drawings
The invention is described with the aid of the following figures:
FIG. 1 is a schematic perspective view of a photobioreactor according to an embodiment of the present invention;
FIG. 2 is a schematic cross-sectional view of a photobioreactor according to an embodiment of the present invention;
fig. 3 is a schematic perspective view of the uppermost photoreaction unit according to an embodiment of the present invention, in which an enlarged view of a connection tube is shown.
[ description of reference ]
1: a support;
11: a photoreaction unit installation site; 12: connecting columns;
2: a photoreaction unit;
21: a transparent serpentine channel; 22: a light guide plate; 23: an LED light bar; 24: a box body; 25: a partition plate; 26: a water outlet hole;
3: a connecting pipe;
31: a main pipeline; 32: branch pipelines; 33: a valve;
4: a floating light intensity detection module;
5: a flexible cord;
6: a pH and inorganic salt concentration monitoring module;
61: an aerator; 62: and a concentrated culture medium filling unit.
Detailed Description
For a better understanding of the present invention, reference will now be made in detail to the present embodiments of the invention, which are illustrated in the accompanying drawings. As used herein, the terms "upper," "lower," "front," "rear," "left," "right," and the like are used with reference to the orientation of FIG. 1.
This example provides a three-dimensional continuous flow type microalgae photobioreactor. As shown in fig. 1 and 2, the photobioreactor includes a support 1 and two or more photoreaction units 2, the support 1 has two or more photoreaction unit installation sites 11 sequentially stacked and spaced from top to bottom, the photoreaction units 2 are fixedly installed on the photoreaction unit installation sites 11, and the two or more photoreaction units 2 are stacked one on another; each photoreaction unit 2 has a transparent serpentine channel 21 and a light guide plate 22 arranged in a horizontal direction, the light guide plate 22 is located right below the transparent serpentine channel 21, an LED light bar 23 is attached to at least one thickness surface of the light guide plate 22, an upper surface of the light guide plate 22 serves as a light emitting surface, and a reflective film is attached to a lower surface and other thickness surfaces of the light guide plate 22. In two adjacent light reaction units 2 in the height direction, the outlet of an upper transparent snake-shaped flow passage 21 is communicated with the inlet of a lower transparent snake-shaped flow passage 21 through a connecting pipe 3; all be provided with floating light intensity detection module 4 in each transparent snakelike runner 21, floating light intensity detection module 4 is connected with snakelike runner inner wall or bottom surface through flexible rope 5, lets in little algae cell suspension in the transparent snakelike runner 21 after, and floating light intensity detection module 4 floats on little algae cell suspension surface to the light intensity sensing probe of floating light intensity detection module 4 hugs closely little algae cell suspension surface.
The working principle of the photobioreactor provided by the embodimentThe following were used: dissolved with CO 2 The microalgae cell suspension enters the transparent snake-shaped flow channel 21 of the light reaction unit 2 at the uppermost layer (namely the first layer) under the driving of the pump and flows along the snake-shaped flow channel, meanwhile, light emitted by the LED light bar 23 enters the light guide plate 22, light transmission is realized by the light guide plate and is emitted along the upper surface of the light guide plate 22, and the light emitted into the transparent snake-shaped flow channel 21 provides illumination for the photoautotrophic growth of microalgae. Then, the microalgae suspension enters the second layer of photoreaction units 2 through the connecting pipe 3, and sequentially flows downwards until the microalgae suspension flows to the last layer of photoreaction units 2. The microalgae suspension flows out of the last layer of photoreaction unit 2 and enters a liquid storage barrel outside the bioreactor, dissolved carbon dioxide is supplemented in the liquid storage barrel, dissolved oxygen is discharged, and then the microalgae suspension reenters the first layer of photoreaction unit 2 under the action of a pump to perform the next cycle.
The photobioreactor that so sets up, in each photoreaction unit 2, through at least one thickness face at light guide plate 22 attached LED lamp strip 23, regard the upper surface of light guide plate 22 as the mere exit face, all attach the reflective membrane with other faces (lower surface and other thickness faces) of light guide plate 22, the emergent light of LED lamp strip 23 gets into inside back of light guide plate 22, utilize these reflective membranes to retrain light at the inside conduction of light guide plate 22, and can follow the even outgoing of upper surface of light guide plate 22, guarantee that the light energy is high-efficient to be utilized and distribute evenly in the transparent snakelike runner 21 that is located light guide plate 22 top, reduce the loss, and then all little algae cells homoenergetic receive sufficient illumination in being favorable to the reactor, simultaneously, the accurate growth regulation of little algae cell in each photoreaction unit 2 provides the basis. More than two photoreaction units 2 are arranged in an up-and-down direction and are mutually stacked, so that the floor area of the photobioreactor is reduced, and the microalgae biomass yield per floor area is increased.
Along with the progress of the culture process, the concentration of microalgae cells in the reactor is increased, a floating light intensity detection module 4 flexibly connected with a flow channel wall is arranged in each transparent snake-shaped flow channel 21, the floating light intensity detection module 4 floats on the surface of a microalgae cell suspension liquid under the action of buoyancy and is pulled by a flexible rope 5 fixed on the bottom surface of the snake-shaped flow channel, the flexible rope 5 can ensure that the floating light intensity detection module 4 is not taken away by the flowing microalgae cell suspension liquid and can also ensure that the floating light intensity detection module 4 always floats on the surface of the microalgae cell suspension liquid, the floating light intensity detection module 4 can detect the light emitted by a light guide plate 22 in real time and finally reaches the illumination intensity on the surface of the suspension liquid, the growth environment of the microalgae cells of the light reaction unit 2 is adjusted in real time based on the measured illumination intensity value, the light emitting power of an LED light bar 23 enables the light intensity measured by the floating light intensity detection module to be equal to or slightly higher than the light compensation point of the microalgae cells, and further ensures that all the microalgae cells in the reaction unit can receive the illumination higher than the light of the organic matter consumed by the photosynthesis of the microalgae cells, thereby greatly improving the biomass reactor.
It should be noted that the electric energy for driving the LED light bar 23 may use electric energy of renewable energy sources such as solar energy, wind energy, and the like, in addition to electric energy of a conventional power grid. It should be noted that, due to the existence of the transparent serpentine channel 21, the microalgae cell suspension in the channel at the edge of the transparent serpentine channel 21 can not only absorb the light energy of the LED, but also directly absorb the sunlight for growing.
Further, in the present embodiment, the rack 1 has connecting columns 12 for connecting to two adjacent photoreaction unit installation sites 11, and the height of the connecting columns 12 is adjustable. Therefore, the distance between the adjacent photoreaction units 2 can be adjusted, and the microalgae biomass yield of unit floor area is conveniently and further improved. Specifically, the connecting column 12 is a positionable telescoping rod.
Preferably, in this embodiment, the light reaction unit 2 includes a box body 24 with an open or closed top, the box body 24 is fixedly installed on the light reaction unit installation position 11, a plurality of partition boards 25 are disposed in the cavity of the box body 24, the cavity of the box body 24 is shaped into the transparent serpentine channel 21 with a unique flow path by the plurality of partition boards 25, and the bottom board of the box body 24 is tightly attached to the upper surface of the light guide plate 22. Alternatively, the bottom plate of the case 24 may also serve as the light guide plate 22, in which case no additional light guide plate is required.
Conceivably, in order to form the transparent serpentine flow channel 21, the box body 24 is a transparent box body 24, and the partition plate 25 is a transparent partition plate 25; specifically, the transparent box 24 and the transparent partition 25 may be made of glass, organic glass, quartz, or the like.
Of course, it is only preferable to set the photoreaction unit 2 as a box body 24 with an open top or a closed top, and it is conceivable that the photoreaction unit 2 may not have the structure of the box body 24, but directly include a transparent serpentine channel, the inside of the transparent serpentine channel forms the transparent serpentine channel 21, and the light guide plate 22 is located right below the transparent serpentine channel, so that a similar effect can be achieved. Specifically, the transparent serpentine pipeline can be made of glass, organic glass, quartz and the like.
Preferably, the inside of the light guide plate 22 is doped with nano-scale light scattering particles. Specifically, in this embodiment, the thickness of the light guide plate 22 is 4mm, and the 2835LED light bars 23 matched with the thickness of the light guide plate 22 are selected for the LED light bars 23. The chambers of the transparent box body 24 form mutually parallel and communicated flow passages under the separation action of the partition plate 25, and the transparent snake-shaped flow passage 21 is constructed in a mode of sealing the bottom and opening the top.
Preferably, the photobioreactor further includes a control unit (not shown in the figure), the floating light intensity detection module 4 is in communication connection with the control unit, and the control unit is electrically connected with the LED light bar 23 of each photoreaction unit 2. So, realize when the light intensity of the light reaction unit 2 that the light intensity detection module surveyed is less than little algae's light compensation point, the power of the LED lamp strip 23 of automatic reinforcing light reaction unit 2 provides more light quantum flux density for little algae cell's photoautotrophic growth in the snakelike runner to the light intensity that makes the light intensity detection module survey is equal to or is a little higher than the light compensation point of algae kind.
Further preferably, in the present embodiment, the LED light bar 23 is attached to each thickness surface of the light guide plate 22, the upper surface of the light guide plate 22 serves as a light emitting surface, and the reflective film is attached to the lower surface of the light guide plate 22. Therefore, the maximum power of the LED lamp strip 23 in the photoreaction unit 2 is improved, the power regulation range of the LED lamp strip 23 is expanded, and the photoautotrophic growth environment of the microalgae cells in the photoreaction unit 2 is regulated conveniently by controlling the power of the LED lamp strip 23.
Further, in this embodiment, two floating light intensity detection modules 4 are disposed in each transparent serpentine channel 21, one floating light intensity detection module 4 is disposed near an inlet of the transparent serpentine channel 21, and the other floating light intensity detection module 4 is disposed near an outlet of the transparent serpentine channel 21. The light intensity of the light reaction unit 2 is detected by arranging the two floating light intensity detection modules 4, so that the detection precision is improved.
Preferably, the connection pipes 3 between two adjacent photoreaction units 2 in the height direction each include a main pipe 31 and a plurality of branch pipes 32; in two adjacent photoreaction units 2, the export of the transparent snakelike runner 21 of upper photoreaction unit 2 is a plurality of apopores 26 that arrange in proper order along the direction of height, and every apopore 26 all communicates through the first end of a pipeline 32 with trunk line 31, and the second end of trunk line 31 communicates with the import of the transparent snakelike runner 21 of lower floor photoreaction unit 2, all is provided with valve 33 on every branch pipeline 32.
So, through opening and closing of valve 33 on the control branch pipeline 32, realized the adjustable of the liquid level degree of depth of little algae cell suspension in every transparent snakelike runner 21, specifically, the valve 33 of the apopore branch pipeline 32 that corresponds the higher position department of liquid degree of depth is closed, and the valve 33 of the less shallow position department apopore branch pipeline 32 of corresponding liquid degree of depth is opened, and then the algae liquid degree of depth in the transparent snakelike runner of photobioreactor reduces. Thereby, the depth of the algae liquid in each layer of the photoreaction unit 2 is adjusted. The liquid level depth of the microalgae cell suspension in the transparent serpentine flow channel 21 is adjusted to control the consumption of the microalgae cells to the LED light energy, and meanwhile, the height of the light intensity detection module 4 is reduced along with the reduction of the depth of the microalgae liquid, so that the light intensity detected by the light intensity detection module 4 is equal to or slightly higher than the light compensation point of the algae species, and further, the situation that all the microalgae cells in the photobioreactor can receive the illumination higher than the light compensation point is ensured.
In summary, the photobioreactor provided by the invention can adjust the illumination intensity on the surface of the suspension through two modes (namely controlling the power of the LED lamp strip 23 and controlling the valve 33 of the branch pipeline 32 to be switched), the adjusting range is wide, and the number of times of the photobioreactor for circularly culturing the microalgae suspension is greatly increased.
Further, the valve 33 is a solenoid valve, and the solenoid valve is electrically connected to the control unit. Thus, the automatic adjustment of the liquid level depth of the microalgae cell suspension liquid in the transparent serpentine flow channel 21 is realized.
Preferably, a pH and inorganic salt concentration monitoring module 6 is arranged in each transparent serpentine flow channel 21, and an aerator 61 and a concentrated culture medium filling unit 62 are integrated on the pH and inorganic salt concentration monitoring module 6. Thus, the pH and inorganic salt concentration monitoring module 6 can monitor the concentrations of inorganic nutrient salts such as pH, nitrate, phosphate and the like in the microalgae cell suspension of the photoreaction unit 2 in real time, when the concentrations of the inorganic nutrient salts such as pH, nitrate, phosphate and the like in the microalgae cell suspension are not within the optimum range of the algae species, the aerator 61 is driven to work to introduce carbon dioxide gas with the volume concentration of 5% into the microalgae cell suspension, and the concentrated medium filling unit 62 is driven to work to introduce the culture medium with the concentration of 5 times into the microalgae cell suspension until the concentrations of the inorganic nutrient salts such as pH, nitrate, phosphate and the like in the microalgae cell suspension measured by the pH and inorganic salt concentration monitoring module 6 are within the optimum growth range of the algae.
It should be understood that the above description of specific embodiments of the present invention is only for the purpose of illustrating the technical lines and features of the present invention, and is intended to enable those skilled in the art to understand the contents of the present invention and to implement the present invention, but the present invention is not limited to the above specific embodiments. It is intended to cover in the appended claims all such changes and modifications that are within the scope of this invention.
Claims (8)
1. A three-dimensional continuous flow type microalgae photobioreactor which is characterized in that,
comprises a bracket (1) and more than two light reaction units (2) which are fixed on the bracket (1) and are sequentially stacked along the vertical direction;
each photoreaction unit (2) is provided with a transparent snake-shaped flow channel (21) and a light guide plate (22) which are arranged in the horizontal direction, the light guide plate (22) is positioned right below the transparent snake-shaped flow channel (21), at least one thickness surface of the light guide plate (22) is attached with an LED lamp bar (23), the upper surface of the light guide plate (22) serves as a light emergent surface, and the lower surface and other thickness surfaces of the light guide plate (22) are attached with a reflective film; in the adjacent photoreaction units (2), the outlet of the upper transparent serpentine channel is communicated with the inlet of the lower transparent serpentine channel through a connecting pipe (3);
each transparent snake-shaped flow channel (21) is internally provided with a floating type light intensity detection module (4), the floating type light intensity detection module (4) is connected with the wall of the snake-shaped flow channel through a flexible rope (5), after microalgae cell suspension is introduced into the transparent snake-shaped flow channel (21), the floating type light intensity detection module (4) floats on the surface of the microalgae cell suspension, and a light intensity sensing probe of the floating type light intensity detection module (4) is tightly attached to the surface of the microalgae cell suspension;
the connecting pipes (3) between two adjacent light reaction units (2) in the height direction respectively comprise a main pipe (31) and a plurality of branch pipes (32); in two adjacent photoreaction units (2), the outlet of the transparent snake-shaped flow channel (21) of the upper photoreaction unit (2) is a plurality of water outlet holes (26) which are sequentially arranged along the height direction, each water outlet hole (26) is communicated with the first end of the main pipeline (31) through a branch pipeline (32), the second end of the main pipeline (31) is communicated with the inlet of the transparent snake-shaped flow channel (21) of the lower photoreaction unit (2), and a valve (33) is arranged on each branch pipeline (32).
2. The stereoscopic continuous flow type microalgae photobioreactor as claimed in claim 1,
the light reaction unit (2) comprises a box body (24) with an opening or a closing top, the box body (24) is fixedly arranged on a light reaction unit mounting position (11) of the support, a plurality of partition plates are arranged in a containing cavity of the box body (24), the containing cavity of the box body (24) is shaped into a transparent snake-shaped flow channel (21) with a unique flow path by the plurality of partition plates, and a bottom plate of the box body (24) is used as a light guide plate (22) or a bottom plate of the box body (24) and is tightly attached to the upper surface of the light guide plate (22);
or, the photoreaction unit (2) comprises a transparent serpentine pipeline, a transparent serpentine flow channel is formed inside the transparent serpentine pipeline, and a light guide plate (22) is arranged below the transparent serpentine pipeline.
3. The stereoscopic continuous flow type microalgae photobioreactor as claimed in claim 1,
still include the control unit, float formula light intensity detection module (4) and be connected with the control unit communication, the control unit is connected with LED lamp strip (23) electricity of each photoreaction unit (2).
4. The stereoscopic continuous flow type microalgae photobioreactor as claimed in claim 1,
each thickness surface of the light guide plate (22) is attached with an LED lamp bar (23), the upper surface of the light guide plate (22) is used as a light emergent surface, and the lower surface of the light guide plate (22) is attached with a reflective film.
5. The stereoscopic continuous flow type microalgae photobioreactor as claimed in claim 1,
a pH and inorganic salt concentration monitoring module (6) is arranged in each transparent snake-shaped flow passage (21), and an aerator (61) and a concentrated culture medium filling unit (62) are integrated on the pH and inorganic salt concentration monitoring module (6).
6. The stereoscopic continuous flow type microalgae photobioreactor as claimed in claim 1,
the bracket (1) is provided with a connecting column (12) used for connecting the installation positions (11) of the adjacent light reaction units, and the height of the connecting column (12) can be adjusted.
7. The stereoscopic continuous flow type microalgae photobioreactor as claimed in claim 1,
the light guide plate (22) is doped with nanoscale light-scattering particles.
8. The stereoscopic continuous flow type microalgae photobioreactor as claimed in claim 1,
the device also comprises a control unit, wherein the valve (33) is an electromagnetic valve, and the electromagnetic valve is electrically connected with the control unit.
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Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102296022A (en) * | 2010-06-25 | 2011-12-28 | 新奥科技发展有限公司 | Optical bioreactor |
| CN204550525U (en) * | 2015-01-20 | 2015-08-12 | 青岛市蓝色海洋科学研究院 | A kind of New-type plate bioreactor |
| CN105368699A (en) * | 2015-12-09 | 2016-03-02 | 重庆大学 | Microalgae photobioreactor with nano light guide plates serving as light dispersion media |
| CN205368378U (en) * | 2015-12-25 | 2016-07-06 | 中国科学院天津工业生物技术研究所 | Microalgae cultivation device |
| CN107012082A (en) * | 2017-04-25 | 2017-08-04 | 北海生巴达生物科技有限公司 | The bioreactor of both culturing microalgae |
| CN107429211A (en) * | 2015-08-14 | 2017-12-01 | 国家开发投资公司 | Stacked thin plate bioreactor |
| CN108018194A (en) * | 2018-01-12 | 2018-05-11 | 华南理工大学 | A kind of duct type bioreactor for microalgae mass culture |
| CN111647501A (en) * | 2020-06-16 | 2020-09-11 | 南京师范大学 | Multilayer stacking adsorption type microalgae biomembrane photobioreactor based on light guide carrier |
| FR3107281A1 (en) * | 2020-02-14 | 2021-08-20 | Fermentalg | REACTOR WITH OPTIMIZED LIGHTING DEVICE |
| CN215179630U (en) * | 2021-06-16 | 2021-12-14 | 杭州清泚源科技有限公司 | Algae monitoring devices |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010056103A2 (en) * | 2008-11-12 | 2010-05-20 | Utar Education Foundation | A photobioreactor |
| WO2018223242A1 (en) * | 2017-06-09 | 2018-12-13 | Jianwei Chen | Production process for high purity algae |
-
2022
- 2022-07-04 CN CN202210788265.7A patent/CN114940941B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102296022A (en) * | 2010-06-25 | 2011-12-28 | 新奥科技发展有限公司 | Optical bioreactor |
| CN204550525U (en) * | 2015-01-20 | 2015-08-12 | 青岛市蓝色海洋科学研究院 | A kind of New-type plate bioreactor |
| CN107429211A (en) * | 2015-08-14 | 2017-12-01 | 国家开发投资公司 | Stacked thin plate bioreactor |
| CN105368699A (en) * | 2015-12-09 | 2016-03-02 | 重庆大学 | Microalgae photobioreactor with nano light guide plates serving as light dispersion media |
| CN205368378U (en) * | 2015-12-25 | 2016-07-06 | 中国科学院天津工业生物技术研究所 | Microalgae cultivation device |
| CN107012082A (en) * | 2017-04-25 | 2017-08-04 | 北海生巴达生物科技有限公司 | The bioreactor of both culturing microalgae |
| CN108018194A (en) * | 2018-01-12 | 2018-05-11 | 华南理工大学 | A kind of duct type bioreactor for microalgae mass culture |
| FR3107281A1 (en) * | 2020-02-14 | 2021-08-20 | Fermentalg | REACTOR WITH OPTIMIZED LIGHTING DEVICE |
| CN111647501A (en) * | 2020-06-16 | 2020-09-11 | 南京师范大学 | Multilayer stacking adsorption type microalgae biomembrane photobioreactor based on light guide carrier |
| CN215179630U (en) * | 2021-06-16 | 2021-12-14 | 杭州清泚源科技有限公司 | Algae monitoring devices |
Non-Patent Citations (3)
| Title |
|---|
| Enhancing CO2 photo-biochemical conversion in a newly-designed attached photobioreactor characterized by stacked horizonal planar waveguide modules;Sun yahui等;《Science of the total environment》;20201210;第760卷;144041第1-9页 * |
| 基于光传递强化及调控的微藻光生物反应器性能强化;孙亚辉;《中国博士学位论文库-基础科学辑》;20190415(第4期);A006-131 * |
| 纳米导光板光生物反应器内微藻生物膜生长特性及其性能强化研究;韩钰莹;《中国优秀硕士论文库-基础科技辑》;20220315(第3期);A006-1166 * |
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