CN106916724B - A kind of scenedesmus cultural method and culture apparatus - Google Patents

A kind of scenedesmus cultural method and culture apparatus Download PDF

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CN106916724B
CN106916724B CN201511001488.0A CN201511001488A CN106916724B CN 106916724 B CN106916724 B CN 106916724B CN 201511001488 A CN201511001488 A CN 201511001488A CN 106916724 B CN106916724 B CN 106916724B
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scenedesmus
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culture apparatus
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CN106916724A (en
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胡强
王宇
韩丹翔
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Sdic Biotechnology Investment Co ltd
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Guo Ding Biotechnology Investment Co Ltd
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Abstract

This application involves microalgae industry application fields, in particular to a kind of scenedesmus cultural method and culture apparatus.

Description

A kind of scenedesmus cultural method and culture apparatus
Invention field
This application involves microalgae industry application fields, in particular to a kind of scenedesmus cultural method and culture apparatus.
Background of invention
Scenedesmus is a kind of unicellular organism, is widely present in Different Waters.Scenedesmus is rich in protein, fat and carbon hydrate Object, metabolite added value is high, can be applied to food, the fields such as medicine, health care, the energy.But nature hardly results in greatly The scenedesmus biomass of amount, this is the principal element for restricting scenedesmus industrialization.Therefore, how on a large scale, low cost culture scenedesmus be The technical issues of by that must be solved before scenedesmus industrial application.
The method of culture scenedesmus mainly uses liquid-immersed method at present, and typical represent has open raceway pond and plate The closed photo bioreactor of the compositions such as the others transparent vessel such as formula, duct type, column.Immersion method is to be immersed in algae In a large amount of culture medium, so that culture medium is continuously in motion state by blender or recycle unit or sparging apparatus, make each Frustule can equably receive illumination and absorb CO2.However, these cultural methods and culture apparatus have, energy consumption is high, cell is close Spend that low (being only capable of accomplishing dry cell weight 2g/l), pollution sources internal communication speed is fast, light utilization ratio is low, occupied space is big, uses water The disadvantages such as amount is big, unit volume space yield is low.In addition, immersion method culture scenedesmus can make the scenedesmus water content of harvest quite big, It has to enter back into spray drying tower after filtering and concentrating or centrifugal concentrating and be spray-dried, this process energy consumption is very high, effect Rate is also very low.
Existing another kind scenedesmus cultural method is semidry method, i.e., algae is attached to sheet water-absorbing material surface, passed through Culture solution is supplied to these water-absorbing materials, grows the algae of attachment equally like moss.These water-absorbing materials can be certain Spacing is hung vertically, and is being laterally located a smooth wall to provide illumination.The benefit of semidry method is without stir culture liquid, relatively Immersion method can save energy consumption；It occupies little space, many water suction diaphragms can be hung in a unit space；And frustule adheres to In membrane surface, the CO in air can be directly utilized2, without being manually passed through CO2.The culture density of semidry method culture scenedesmus is reachable Dry cell weight 50g/m2/d.However, water-absorbent material (such as canvas, cotton) surface of semidry method is very easy to mildew.This be because It coincide very much for the environment where the growing environment and water-absorbing material film of mould, the mould speed of growth is even more than the speed of algae Degree.Once catching mould, rotten denaturation can all occur whithin a period of time and even rot to be crushed for monolith water-absorbing material.Do not have at present Have to the remaining mould processing method of algae nonhazardous.In addition, semidry method training method needs continuously or discontinuously cultivate liquid pump It is sprayed down to water-absorbent material top, also there is no small energy requirements.
Therefore, this field still needs new scenedesmus cultural method and device.
Summary of the invention
On the one hand, the present invention provides a kind of stacked thin plate scenedesmus culture apparatus, including are laminated on vertical direction Multiple thin plates of arrangement, the multiple thin plate are supported so that being spaced a preset distance between adjacent thin plate, each thin plate is along water Square to extension, there are opposite upper and lower surfaces, and be provided peripherally with from the upper surface of corresponding thin plate and upwardly extend Cofferdam, the upper surface of each thin plate and the inner surface of cofferdam form the space for accommodating scenedesmus culture solution, wherein described The vertical height of the minimum of cofferdam is not more than 15mm.
In some embodiments, multiple support columns are set between the adjacent thin plate of up and down direction, the support column is used for The thin plate of support above.
In some embodiments, the cofferdam is integrally formed with corresponding thin plate, or after independently forming it is attached It is connected to corresponding thin plate.
In some embodiments, the support column is integrally formed with corresponding thin plate, or after independently forming it is attached It is connected to the lower surface of corresponding thin plate.
In some embodiments, the cofferdam is relative to the upper surface of corresponding thin plate at inclining more than or equal to 90 degree Angle.
In some embodiments, the cofferdam is higher by the upper surface 1.5mm-15mm of corresponding thin plate, such as 1.5mm- 4.5mm, 6mm-9mm or 10.5-15mm.
In some embodiments, the cofferdam is made and/or the support of the material identical or different with thin plate Column is made of the material identical or different with thin plate.
In some embodiments, the thin plate is made of one of materials described below: glass, GPPS, transparent ABS, AS (benzene second Alkene acrylonitrile), PVC, PMMA (polymethyl methacrylate), PC (polycarbonate), PS (polystyrene).
In some embodiments, the thin plate is made of plate glass, and the cofferdam is attached to thin plate Adhesive tape, preferably cellophane adhesive tape form the receiving of a culture solution in one circle cellophane adhesive tape of the surrounding bonding of the plate glass Space.
In some embodiments, the thin plate is made of organic material, and the cofferdam and thin plate are integrally formed.
In some embodiments, the spacing between each thin plate of up and down direction is identical or different, between adjacent sheet between Away from can be 5mm-25mm, such as 5mm-9mm or 11-25mm, preferably 5mm.
In some embodiments, the side of the scenedesmus culture apparatus is provided with light source.
On the other hand, the present invention provides a kind of scenedesmus cultural methods, including static in thin layer fluid nutrient medium Cultivate the scenedesmus, wherein the thin layer fluid nutrient medium with a thickness of 1mm-10mm, such as 1mm-2.5mm, 3.5mm- 4.5mm or 5mm-10mm.In some preferred embodiments, the thin layer fluid nutrient medium is with a thickness of 1mm-2.5mm.One In a little embodiments, the scenedesmus is tapering scenedesmus (Scenedesmus acuminatus).
In some embodiments, environment temperature used in the scenedesmus culture is 20-30 DEG C.
In some embodiments, light intensity used in the scenedesmus culture is 1-238umolm-2·s-1。
In some embodiments, culture medium used in the scenedesmus culture is BG11 culture medium.
In some embodiments, the ambient humidity range in the scenedesmus culture is 80-99%.
In some embodiments, the density of scenedesmus initial inoculation is 0.1-0.6OD in the scenedesmus culture750。
In some embodiments, the scenedesmus culture carries out in stacked thin plate scenedesmus culture apparatus of the invention. In some embodiments, multiple stacked thin plate scenedesmus culture apparatus can be arranged to array in the horizontal direction, wherein The side of array is disposed with light source.
Brief description
Fig. 1 is the perspective view of stacked thin plate scenedesmus culture apparatus according to the preferred embodiment of the invention.
Fig. 2 is the front view of Fig. 1.
Fig. 3 is the cross-sectional view of the A-A along Fig. 1.
Fig. 4 A is the top view of apparatus of the present invention array, wherein the light source of left side setting 1m × 1m, along the light direction of propagation 10 row's the device of the invention are set.
Fig. 4 B is the top view of classic flat-plate vessel type reactor, wherein the light source of left side setting 1m × 1m.
Fig. 5 shows the scenedesmus biomass that apparatus of the present invention array is respectively arranged the light intensity value of media surface and finally harvested.
Detailed description of the invention
The present invention provides a kind of scenedesmus cultural methods, including in the thin layer fluid nutrient medium with a thickness of 1mm-10mm Scenedesmus described in static culture.In some embodiments, the scenedesmus is tapering scenedesmus (Scenedesmus acuminatus).
Since the thickness of culture medium in method of the invention is sufficiently small (1mm-10mm), do not need to be stirred etc. Matterization operation still is able to guarantee that all scenedesmus cells in culture solution more can equably obtain illumination (in light to water In the effective distance of lower transmitting).And by using culture medium thickness of the present invention (1mm-10mm), so that scenedesmus cell CO required for photosynthesis can be directly obtained from the liquid level of culture solution by gas exchanges2With and discharge to photosynthesis produce The raw O inhibited2, therefore do not need to carry out aeration to culture solution.
Frond harvest and drying are the important steps of algae culture, and energy consumption is high, low efficiency.However, according to the present invention Method, using thin layer fluid nutrient medium static culture scenedesmus, when algae grows to maturation (generally about 8-9 days), culture Base is usually dissipated major part.At this point, dry cell weight has been reached compared with ratios, it is very beneficial for frond harvest, drying.In this hair It, can be according to factors such as scenedesmus inoculum concentration, light intensity, humidity and temperature to culture within the scope of bright culture medium thickness (1mm-10mm) The thickness of base is adjusted, so that the amount of culture medium grows into the maturity period without having excessive have more than needed for algae just.In addition, Since frond is constantly in Thin cell layer liquid, it is less susceptible to bacterium of mildewing.
Culture medium thickness used in method of the invention can be about 1mm, 1.5mm, 2mm, 2.5mm, 3mm, Range between 3.5mm, 4mm, 4.5mm, 5mm, 6mm, 7mm, 8mm, 9mm, 10mm or above-mentioned arbitrary value.For example, the thickness It can be 1mm-2.5mm, 3.5mm-4.5mm or 5mm-10mm.Preferably, described with a thickness of 1mm-2.5mm.
In some embodiments, temperature used in the scenedesmus cultural method is 20-30 DEG C.In some embodiments In, light intensity used in the scenedesmus culture is 1-238umolm-2·s-1.In some embodiments, the scenedesmus culture Used in culture medium be BG11 culture medium.In some embodiments, the ambient humidity range in the scenedesmus culture is 80- 99%.In some embodiments, the density of scenedesmus initial inoculation is 0.1-0.6OD in the scenedesmus culture750, wherein OD750 It is the absorbance measured under 750nm wavelength.
Scenedesmus cultural method of the invention is by the way of thin layer static culture, and its essence is between immersion method and semidry method Between a kind of novel scenedesmus cultural method, as described above, have energy saving, reduce toxigenic capacity, reduce mould, other enemy The advantage of evil plankton pollution risk.
On the other hand, the present invention also provides a kind of stacked thin plate scenedesmus culture apparatus comprising in vertical direction Upper stacking and the multiple thin plates arranged in preset space length, the multiple thin plate is horizontally extending, and each thin plate, which has, to be used for The upper surface and opposite lower surface of scenedesmus culture solution are placed, the periphery of each thin plate is equipped with to be favoured from what corresponding thin plate stretched out The cofferdam that the upper surface of corresponding thin plate upwardly extends.Wherein, the vertical height of the minimum of the cofferdam is not more than 15mm.
Fig. 1 to 3 shows stacked thin plate scenedesmus culture apparatus 100 according to the preferred embodiment of the invention.
It can be seen from the figure that stacked thin plate scenedesmus culture apparatus 100 includes the more of the arranged stacked on vertical direction A thin plate 20 and multiple support columns 40 between adjacent thin plate.Each thin plate 20 is horizontally extending, and has and use In the upper surface 22 and opposite lower surface 24 of placing scenedesmus culture solution.The periphery of each thin plate 20 is equipped with stretches from corresponding thin plate 20 The cofferdam 26 that the upper surface 22 for favouring corresponding thin plate 20 out upwardly extends.
Each thin plate 20 forms the culture plate of culture apparatus, that is, the upper surface 22 of each thin plate 20, the cofferdam 26 of thin plate 20 Inner surface limits the space 30 for accommodating scenedesmus culture solution.
The transparent plate made of glass material or organic material can be used in the thin plate 20 for forming culture apparatus.Relative to pipe For shape, channel-shaped or can-like culture apparatus, the scenedesmus culture apparatus processing and manufacturing according to the present invention being made of plate Simply, at low cost, and can accomplish that the culture area of single culture plate is larger, thickness can also be relatively thin, and transparency can With higher.The length of the thin plate for example can be 10cm-10m, and width for example can be 5cm-1m.Each thin plate 20 is in vertical side Upward arranged stacked keeps structure integrated, and effective culture area of unit space occupied maximizes.
Since the material of each thin plate 20 is the high material of transparency, in the Initial stage of culture of scenedesmus, due on each thin plate 20 Dark biomembrane has not yet been formed in scenedesmus, therefore can reach adjacent lower layer through each 20 upper surface of layer thin plate from external light , equally can also be from the culture liquid level that the lower surface of each layer thin plate 20 reaches upper layer thin plate on the culture liquid level of thin plate, illumination Efficiency is higher.Light source is irradiated to below from the frustule above the upper surface direct irradiation of thin plate 20 and through thin plate 20 Thin plate 20 upper surface 22 on the cell cultivated, so light-receiving area is big, light utilization ratio is very high.
Using the scenedesmus culture apparatus of this structure, the water body used is few, so the culture of scenedesmus saves energy without stirring Consumption.In addition, since water is few, when harvest, can reduce process and thus reduce harvest cost.Thus, utilize the grid of this structure Algae culturing device culture scenedesmus is static gas wave refrigerator, is not necessarily to convey gas in incubation or provides stirring power, and can be well Obtain illumination.
According to the present invention, the cofferdam 26 of each thin plate 20 can be integrally formed with thin plate 20, after can also independently forming It is attached to thin plate 20.
When thin plate 20 is made of glass material, cofferdam 26 can be the circle adhesive tape for being adhered to 20 periphery of thin plate, excellent Select cellophane adhesive tape.For the thin plate 20 of organic material, cofferdam 26 can be integrally formed with thin plate 20.
The material for forming thin plate 20 can be glass or the high plastics of light transmittance, such as GPPS, transparent ABS, AS (styrene third Alkene nitrile), PVC, PMMA (polymethyl methacrylate), PC (polycarbonate), PS (polystyrene) etc..Form the material of cofferdam 26 Material can be material identical with thin plate 20, can also use other transparent materials, such as adhesive tape, glass cement.
The upper surface 22 of cofferdam 26 and thin plate 20 is at the angle for being greater than or equal to 90 degree, in order to train on upper surface 22 Feeding scenedesmus is gone out with high pressure water/air-blowing to outside thin plate 20, to realize the harvest of scenedesmus；Preferably cofferdam 26 and thin plate 20 upper surface 22 at the angle greater than 90 degree and forms fillet between the two junction, prevents from forming harvest dead angle or clear Clean dead angle.Optionally, the cofferdam 26 be higher by the upper surface 22 of corresponding thin plate 20 distance be 1.5mm, 3mm, 4.5mm, Range between 6mm, 7.5mm, 9mm, 10.5mm, 12mm, 13.5mm, 15mm or above-mentioned arbitrary value.For example, the distance can To be 1.5mm-4.5mm, 6mm-9mm or 10.5-15mm.In a preferred embodiment, the distance is 1.5mm-3mm.
Spacing (table on vertical range or two neighboring thin plate between two neighboring thin plate lower surface between each thin plate 20 Vertical range between face) it is required according to light source arrangement, the culture of scenedesmus and harvest to determine.For example, can for 5mm, 7mm, Range between 9mm, 11mm, 13mm, 15mm, 20mm or 25mm or above-mentioned arbitrary value.For example, the spacing can be 5-9mm Or 11-25mm.In a preferred embodiment, the spacing is 5mm.
Shape, material, quantity, spacing and the arrangement of support column 40 can not limit, and support column 40 is only needed to have Some strength, the culture solution being supported on the thin plate 20 and its upper surface 22 of stacked on top enough.Preferably, support column 40 uses Transparent material, such as glass, acrylic, PC material.Support column 40 can have any suitable shape, including but not limited to round Cylindricality, tubular, rectangular, strip etc..
In the illustrated embodiment, support column 40 is arranged in banks.Additionally optionally, it is provided on support column 40 for making light The hole that can be penetrated.
For sheets of glass 20, support column 40 can use glass column, be also possible to non-glass column.Support column 40 can be with It is all separated with above and below thin plate 20, the lower surface 24 of thin plate 20 above can also be adhered to.
For the thin plate 20 made of organic transparent material, support column 40 can be integrally formed with thin plate 20.
Stacked thin plate scenedesmus culture apparatus of the invention, which may further be provided, to supplement culture solution to each thin plate 20 Culture solution drop instillator 50, culture solution drop instillator 50 diagrammatically illustrates in Fig. 3, and optionally, configurable measurement The leve monitor (not shown) of the accurate liquid level of each thin plate 20.In addition light source 60 is shown in FIG. 3.Light source can be arranged in The stacked thin plate scenedesmus culture apparatus side of the present invention.
Additionally optionally, stacked thin plate scenedesmus culture apparatus of the invention can further include by stacked thin plate scenedesmus culture The light-transmissive film cover that device is encapsulated provides steady temperature, air humidity for its internal stacked thin plate scenedesmus culture apparatus Atmosphere.This can reduce the evaporation of moisture, create most appropriate growth conditions to the culture of scenedesmus, so can be in scenedesmus In cultivation cycle, the culture solution that replenishes the supply is no longer needed to.
Other than the stacked thin plate scenedesmus culture apparatus of above-mentioned example, each thin plate 20, which can be also individually positioned in, to be had On the bracket of several cross bars (no setting is required at this time support column 40), the weight of each thin plate 20 and culture solution is dispersed by the bracket Power；It equally can achieve technical effect identical with previous examples.
Present invention scenedesmus cultural method above-mentioned can carry out in stacked thin plate scenedesmus culture apparatus of the invention, make Scenedesmus can be cultivated at low cost with scale by obtaining.
In some embodiments of scenedesmus cultural method, multiple stacked thin plate scenedesmus culture apparatus can be in level Direction is arranged to array.In some embodiments, wherein the side in array is disposed with light source.
High density stacking culture is carried out to scenedesmus using culture apparatus of the invention, due to having gap (branch between every layer Dagger height be 3-20mm), propagate to larger distance for light, therefore, light can and in the range of can be used to train Support scenedesmus.Therefore the yield of biomass of unit area light source is directly increased, substantially increases the efficiency of light energy utilization.In addition, multiple Culture apparatus is horizontally disposed at array, and since cofferdam separates each thin plate, and algae solution is in static culture, can to train The pollution being likely to occur in supporting is limited in lesser range, is also convenient for the control to culture environment on single thin plate.
Use the device of the invention culture scenedesmus, it is assumed that the thickness of each thin plate is 1.9mm, and the height of support column About 3mm-20mm, culture solution is with a thickness of 2.5mm, then can stack 45-204 layers under 1m height space.If thin in each layer Upper in the cofferdam of plate to cultivate scenedesmus, then the biomass that can be cultivated in 1m altitude range is considerable.It is implemented as follows in the present invention It can reach dry cell weight 1940g/m in example3, the speed of growth is up to 90g/m2/ d, optical energy utilization efficiency is up to 31.04%, unit mass Scenedesmus energy consumption be only about 1089J/g.
Embodiment
In order to further display the advantageous effects of cultural method and culture apparatus of the invention, following embodiment pair " unit area light source yield " " unit mass algae powder energy consumption " two factors are investigated.
Experimental group:
Using the tapering scenedesmus of culture apparatus culture (Scenedesmus acuminatus) of the invention for being arranged to array (being purchased from the Chinese Academy of Sciences, aquatic institute's algae library, Wuhan).The array top view is shown in Fig. 4 A.
Left side is that length is 1m, is highly the light source of 1m, light intensity 238umolm-2·s-1.Right side is several present invention trainings Support the array of device composition.10 row's culture apparatus are arranged on the direction vertical with light source.Wherein, each thin plate (glass plate) rule Lattice are 120mm × 60mm, and with a thickness of 1.9mm, support column is 3mm, is laminated to 1m in vertical direction.The height in cofferdam is 2.5mm.Each thin plate is paved with scenedesmus culture solution.Each open ended algae solution amount of thin sheet surface is 18ml.Environment temperature control 25 degree or so, relative humidity 80%-99%.Initial inoculation OD on each thin plate750=0.4.Culture is in completely obscured natural light Environment in carry out, exclude natural light interference.
Detected through light-intensity test instrument, from apart from lamp source it is farthest the 10th drain into culture medium in the 1st nearest row's culture apparatus The light intensity on surface is shown in Fig. 5.From fig. 5, it can be seen that existing between layers a large amount of due to there is support column between the thin plate of stacking Tiny spacing, light can be continuously passed on by the gap of interlayer toward distal side, and remoter local light intensity is weaker.Fig. 5 from Left-to-right is successively the scenedesmus biomass that the 10th row, the light intensity value of the 1st row's media surface of the 9th row ... and each row finally harvest (situation of culture 6 days).
And it is observed that the sheets of glass of the 1st row since close to light source, upgrowth situation is best, is grown further away from light source lamp plate Situation is poorer, and the growing state of the 10th row is worst.Wait be harvested after cultivating about 6 days, each row's scenedesmus total biomass is counted, Growing state is as shown in Figure 5.It can therefrom see, although the scenedesmus growing state of the 10th row is poor, can still obtain The irradiation and growth of light source.The number of rows arranged on the direction vertical with light source can be adjusted according to the intensity of light source, and thin plate Between interval can also be adjusted according to the intensity of light source so that unit area light source can get maximum biomass scenedesmus.
It is 593.64g by the scenedesmus gross dry weight harvested in the array of this experiment.Culture solution total volume V=(1000/120) × 10 × 18ml × (1000/4.9)=306.12L, then calculating the algae dry weight density averagely harvested is 1.94g/L.
Control group:
It is carried out in the environment of completely obscured natural light using classic flat-plate vessel type reactor (goldfish jar formula reactor) Scenedesmus culture.As shown in the top view of Fig. 4 B, the traditional plate container of the light source of 1 ㎡ irradiation (identical as light source used in experimental group) One side of type reactor, plate container side are 1 ㎡, container width 5cm.Classic flat-plate vessel type reactor needs stir Mix and stir special CO2Pressure pan gas supply.
In the case where completely obscured natural light, after the light of above-mentioned 1 ㎡ light source lamp plate passes through 5cm algae solution, light intensity is violent Weaken, loss late reaches 96% or more, and the light source of 1 ㎡ is caused to be only capable of for the scenedesmus growth in one plate container of irradiation, nothing Method supplies the scenedesmus growth in another goldfish jar formula bioreactor of located side by side.The volume of plate container is 1 ㎡ * 0.05m=50L.By culture in about 6 days, for goldfish jar formula bioreactor in optimal culture effect, algae dry weight was close Degree is about 2.11g/L, then the algae total amount harvested is 105.5g.
As a result compare:
It is trained as described above using stacked thin plate scenedesmus culture apparatus of the invention and traditional plate container type reactor The result for supporting scenedesmus is summarized as follows table 1 (using 1 ㎡ light source as references object):
Table 1
The present invention Plate container type
Unit area light source yield 98.94g/m2/d 17.58g/m2/d
The energy consumption of unit mass algae powder 57.7KJ/g algae 345.3KJ/g algae
The result being calculated when the above are support column height being 3mm.It and is 5mm, 10mm, 15mm, 20mm in support column When unit area light source yield be respectively 98.46g/m2/ d, 61.96g/m2/ d, 34.59g/m2/d、27.04g/m2/d、 22.19g/m2/d.It can be seen that under above-mentioned various support column heights, the yield of unit area is equal using culture apparatus of the invention More traditional plate container is much higher.But if spacing is too small, the algae solution sheets of glass easy to stick for connecting one layer influences grid The growth of algae, therefore preferably spacing is not less than 5mm.
As it can be seen that the method and apparatus that scenedesmus cultural method of the invention and device are significantly better than the prior art.In addition, this hair Bright cultural method and device are to cultivate in a static manner, during which without for high pressure CO2Air-flotation type stirring is realized, without circulation The equipment such as pump, agitating paddle, further reduce energy consumption.In addition, the present invention uses Thin cell layer, the scenedesmus of harvest has been in semi-moist State (dry weight of harvest test is 540g/ ㎡), after further reducing harvest, then the energy consumption of programs such as is concentrated, is dried.

Claims (22)

1. a kind of stacked thin plate scenedesmus culture apparatus, characterized in that the stacked thin plate scenedesmus culture apparatus is included in perpendicular Multiple thin plates of the upward arranged stacked of histogram, the multiple thin plate are supported so that being spaced a pre- spacing between adjacent thin plate From each thin plate is horizontally extending, has opposite upper and lower surfaces, and be provided peripherally with from the upper of corresponding thin plate The cofferdam that surface upwardly extends, the upper surface of each thin plate and the inner surface of cofferdam form the sky for accommodating scenedesmus culture solution Between, wherein the vertical height of the minimum of the cofferdam is not more than 15mm, wherein the cofferdam is higher by the upper surface of corresponding thin plate 1.5mm-4.5mm。
2. stacked thin plate scenedesmus culture apparatus according to claim 1, characterized in that set between the adjacent thin plate of up and down direction Multiple support columns are set, the support column is used to support thin plate above.
3. stacked thin plate scenedesmus culture apparatus according to claim 1 or 2, characterized in that the cofferdam and corresponding thin plate It is integrally formed, or is attached to corresponding thin plate after independently forming.
4. stacked thin plate scenedesmus culture apparatus according to claim 1 or 2, characterized in that the support column and corresponding thin plate It is integrally formed, or is attached to the lower surface of corresponding thin plate after independently forming.
5. stacked thin plate scenedesmus culture apparatus according to claim 1 or 2, characterized in that the cofferdam is relative to corresponding The upper surface of thin plate is at the inclination angle for being greater than or equal to 90 degree.
6. stacked thin plate scenedesmus culture apparatus according to claim 1 or 2, characterized in that the cofferdam by with thin plate phase Together or different materials is made and/or the support column is made of the material identical or different with thin plate.
7. stacked thin plate scenedesmus culture apparatus according to claim 1 or 2, characterized in that the thin plate by materials described below it One is made: glass, GPPS, transparent ABS, AS (styrene-acrylonitrile), PVC, PMMA (polymethyl methacrylate), PC (poly- carbon Sour rouge), PS (polystyrene).
8. stacked thin plate scenedesmus culture apparatus according to claim 1, characterized in that the thin plate is made of plate glass, And the cofferdam is attached to the adhesive tape of thin plate.
9. stacked thin plate scenedesmus culture apparatus according to claim 8, wherein the adhesive tape is cellophane adhesive tape.
10. stacked thin plate scenedesmus culture apparatus according to claim 1, characterized in that the thin plate is made of organic material, And the cofferdam and thin plate are integrally formed.
11. stacked thin plate scenedesmus culture apparatus according to claim 1 or 2, characterized in that the spacing between each thin plate is identical Or it is different, the spacing between adjacent sheet is 5mm-25mm.
12. stacked thin plate scenedesmus culture apparatus according to claim 11, the spacing between adjacent sheet be 5mm-9mm or 11-25mm。
13. stacked thin plate scenedesmus culture apparatus according to claim 11, the spacing between adjacent sheet is 5mm.
14. a kind of scenedesmus cultural method, including scenedesmus described in the static culture in thin layer fluid nutrient medium, wherein described thin Layer fluid nutrient medium with a thickness of 1mm-4.5mm, wherein stacked thin plate of the scenedesmus culture in any one of claim 1-13 It is carried out in scenedesmus culture apparatus.
15. method according to claim 14, wherein the thin layer fluid nutrient medium with a thickness of 1mm-2.5mm or 3.5mm- 4.5mm。
16. method according to claim 14, wherein the scenedesmus is tapering scenedesmus (Scenedesmus acuminatus).
17. the method for any one of 4-16 according to claim 1, wherein the culture carries out at 20-30 DEG C.
18. the method for any one of 4-16 according to claim 1, wherein the culture is in 1-238umolm-2·s-1Light intensity Lower progress.
19. the method for any one of 4-16 according to claim 1, wherein the culture medium is BG11 culture medium.
20. the method for any one of 4-16 according to claim 1, wherein the culture carries out under the humidity of 80-99%.
21. the method for any one of 4-16 according to claim 1, wherein the density of the initial inoculation of scenedesmus is 0.1-0.6OD750。
22. method according to claim 14, plurality of stacked thin plate scenedesmus culture apparatus is arranged in the horizontal direction Array, wherein the side in array is disposed with light source.
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CN2257291Y (en) * 1996-06-25 1997-07-02 华南理工大学 Enclosed circulating thin layer spirulina cultivating apparatus
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CN102296022A (en) * 2010-06-25 2011-12-28 新奥科技发展有限公司 Optical bioreactor
CN102482634A (en) * 2009-09-15 2012-05-30 拜尔材料科学有限公司 Photobioreactor for algae growth
CN106467902A (en) * 2015-08-14 2017-03-01 国家开发投资公司 A kind of cultural method of photosynthetic microorganism and bioreactor

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Publication number Priority date Publication date Assignee Title
US4741839A (en) * 1986-07-16 1988-05-03 Heritage Industries Inc. Ultrasonic vibrator tray processes and apparatus
CN2257291Y (en) * 1996-06-25 1997-07-02 华南理工大学 Enclosed circulating thin layer spirulina cultivating apparatus
CN102224236A (en) * 2008-11-12 2011-10-19 拉曼大学教育基金会 A photobioreactor
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