CN107421919A - Double light path transmissions and the method for the packed complicated solution component content of fluorescence spectral measuring - Google Patents
Double light path transmissions and the method for the packed complicated solution component content of fluorescence spectral measuring Download PDFInfo
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- CN107421919A CN107421919A CN201710529458.XA CN201710529458A CN107421919A CN 107421919 A CN107421919 A CN 107421919A CN 201710529458 A CN201710529458 A CN 201710529458A CN 107421919 A CN107421919 A CN 107421919A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6491—Measuring fluorescence and transmission; Correcting inner filter effect
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Abstract
The invention discloses a kind of double light path transmissions and the method for the packed complicated solution component content of fluorescence spectral measuring, including：Light source includes transmitted light source and fluorescence excitation light source, and transmitted light source transmits to the complicated solution in packaging bag, and fluorescence excitation light source excites to the complicated solution in packaging bag；Displacement platform controls light source movement, spectrum acquisition device acquisition of transmission spectrum and fluorescence spectrum under the premise of ensureing that light source light extraction optical port and spectrum acquisition device entrance slit are coaxial；Ask logarithm to be absorbed spectrum light intensity ratio under each wavelength of transmitted spectrum under two light paths, and combine two fluorescence spectrums normalized together, the Comparative result with existing chemical analysis, founding mathematical models；Gather the transmitted spectrum and fluorescence spectrum under unknown two light paths of packed complicated solution, the ratio of two transmitted spectrums is asked and brings mathematical modeling into after the absorption spectrum and two fluorescence spectrums normalization that logarithm obtains and calculates, obtains the content that complicated solution surveys target component.
Description
Technical field
The present invention relates to spectrum complicated solution concentration analysis stoichiometry field, more particularly to a kind of double light path transmissions and glimmering
The method of the packed complicated solution component content of light spectral measurement.
Background technology
In the prior art, more ripe mode is to detect containing for complicated solution composition in packaging bag by chemical analysis
Amount, there are the high outstanding advantages of accuracy, but the mode of chemical analysis can not meet quick, non-contact and free of contamination need
Ask, spectral measurement is also possible to realize that complicated solution surveys target component in packaging bag by its non-contact, free of contamination characteristic
Content detection.
In spectral detection, according to Lambert-Beer's law：The incident intensity I of each wavelength is measured respectively0And output intensity
I, the absorbance A of each wavelength is calculated by formula (1).∈ is material absorptivity under a certain wavelength, and c is the dense of material
Degree, b is optical path length.
In fact, fail to measure incident intensity I for various reasons0, such as：Incident intensity I0It is difficult to measure by force very much, but
If in incident intensity I0It is basicly stable it is constant in the case of, only measure an output intensity I can also obtain good result.But
Influence that spectral detection changes due to light source and measure influences of container and be difficult to reach the precision for measuring needs.
The influence of light source is mainly shown as the change of spatial distribution and light intensity.The reason for causing light source to change, has a lot, such as
Light source voltage change, filament aging, variation of ambient temperature etc..In spectrum analysis, rarely have document to introduce light source to measurement accuracy
Influence, and reduce the intensity of light source change on measurement accuracy influence method.In research in the early time, with the mode of calibration come
Some interference are eliminated, are such as calibrated with water, but because light intensity is too strong, are difficult to operate in practice.Also have in many scholars' utilizations
Property attenuator or optical fibre light splitting mode measure incident intensity I0.By taking neutral filter as an example (following discussion unless stated otherwise,
Discussed on some wavelength), the output intensity I that measurement passes through neutral filtern, then the light intensity I of light source0 nAbsorbance can be used
A and output intensity InTo represent：
Then sample is replaced into neutral filter, measures the output intensity I of sampleS,
NoticeSo
Formula does not have in (4)(namely) occur, illustrate that the intensity (and its spectrum) of light source does not interfere with the survey to sample
Amount, as long as all measurements are all calibrated using same neutral attenuator, that is, keep lgIn+AnFor constant constant.
But different occasions are difficult to find duplicate neutral filter, and it is difficult to ensure that the position of sample and neutral filter
Put unanimously, add difficulty to measurement and be difficult to ensure that measurement accuracy.
For the complexity of complicated solution, simple transmitted spectrum specific aim is poor, for further improve complicated solution into
Point measurement accuracy, with reference to fluorescence it is with strong points the characteristics of, but by fluorescence excitation light intensity, optical length and surveyed constituent concentration
Influence, cause fluorescence to have serious self-absorption problem, so as to cause obtained fluorescence spectrum have it is very strong non-linear, it is impossible to
The feature of material is surveyed in reaction well.
The content of the invention
The invention provides a kind of double light path transmissions and the method for the packed complicated solution component content of fluorescence spectral measuring, institute
The method of stating adds the information content for the material that is stimulated, and not only eliminates the influence that transmitted light source change is brought with bag-like container, and
Measure with strong points, greatly inhibit the non-linear effects of spectrum, improve the precision that complicated solution surveys component content analysis.
The measurement of quick, free of contamination packed complicated solution composition is realized, it is workable.It is described below：
A kind of double light paths transmission and the method for the packed complicated solution component content of fluorescence spectral measuring, methods described include with
Lower step：
The light extraction optical port of light source is close to packaging bag and coaxial with the entrance slit of spectrum acquisition device, and light source includes transmitted light
Source and fluorescence excitation light source, transmitted light source transmit to the complicated solution in packaging bag, and fluorescent exciting source excitation complexity is molten
Liquid produces fluorescence, spectrum acquisition device acquisition of transmission spectrum and fluorescence spectrum；
Displacement platform is ensureing that transmitted light source and fluorescence excitation light source light extraction optical port and spectrum acquisition device entrance slit are same
Under the premise of axle, transmitted light source and fluorescence excitation light source movement are controlled, by spectrum acquisition device acquisition of transmission spectrum and fluorescence spectrum；
Ask logarithm to be absorbed spectrum light intensity ratio under each wavelength of two opening position transmitted spectrums, and combine two
Fluorescence spectrum normalized under position, the Comparative result with existing chemical analysis, founding mathematical models；
The transmitted spectrum and fluorescence spectrum at unknown packed complicated solution two positions are gathered, the ratio of two transmitted spectrums is asked
The absorption spectrum and two fluorescence spectrums that logarithm obtains are brought mathematical modeling into after normalizing and calculated, and obtain complicated solution
The content of surveyed target component；
The transmitted spectrum and fluorescence spectrum are to cause the spectrum under the nonlinear factor collective effect of spectrum, and increase is excited
The information content of stimulating food matter；Eliminate the influence that transmitted light source change is brought with bag-like container；Suppress the non-linear effects of spectrum；Improve
Complicated solution surveys the precision of component content analysis.
Wherein, displacement platform control light source movement, the step of by spectrum acquisition device acquisition of transmission spectrum and fluorescence spectrum
Specially：
The complicated solution in packaging bag is transmitted and swashed respectively by transmitted light source and fluorescence excitation light source at a of position
Hair, by spectrum acquisition device acquisition of transmission spectrum and fluorescence spectrum；
Displacement platform control light source is moved to position b, by spectrum acquisition device acquisition of transmission spectrum and fluorescence spectrum；
Or,
Transmitted light source and fluorescence excitation light source are transmitted and excited respectively to the complicated solution in packaging bag, are connect by spectrum
Receiving apparatus acquisition of transmission spectrum and fluorescence spectrum at a of position；
Displacement platform control spectrum acquisition device is moved to position b, gathers transmitted spectrum and fluorescence spectrum at the b of position；
Or,
The complicated solution in packaging bag is transmitted and swashed respectively by transmitted light source and fluorescence excitation light source at a of position
Hair, at position a ' places by spectrum acquisition device acquisition of transmission spectrum and fluorescence spectrum；
Displacement platform controls transmitted light source and fluorescence excitation light source to be moved to position b, and spectrum acquisition device is moved to position
B ' places, by spectrum acquisition device acquisition of transmission spectrum and fluorescence spectrum.
Methods described also includes：
One optical fiber is set at light source, as incident optical, and ensures incident optical and spectrum acquisition device entrance slit
It is close to packaging bag and coaxial；
Or,
One optical fiber is set at spectrum acquisition device, as the output optical fiber, and ensures the output optical fiber and light source light extraction optical port
It is close to packaging bag and coaxial；
Or,
Incident optical and the output optical fiber are set respectively at light source and spectrum acquisition device, and ensure incident optical and outgoing
Optical fiber is close to packaging bag and coaxial.
Wherein, a positions are the first position of incident optical, and incident optical and the output optical fiber phase are gathered by spectrum acquisition device
To the transmitted spectrum and fluorescence spectrum under a of position；Displacement platform is controlled on the premise of ensureing that incident optical and the output optical fiber are coaxial
Incident optical processed is moved at the b of position, and incident optical and the transmission under the output optical fiber relative position b are gathered by spectrum acquisition device
Spectrum and fluorescence spectrum.
Wherein, a positions are the first position of the output optical fiber, and incident optical and the output optical fiber phase are gathered by spectrum acquisition device
To the transmitted spectrum and fluorescence spectrum under a of position；Displacement platform is controlled on the premise of ensureing that incident optical and the output optical fiber are coaxial
The output optical fiber processed is moved at the b of position, and incident optical and the transmission under the output optical fiber relative position b are gathered by spectrum acquisition device
Spectrum and fluorescence spectrum.
Wherein, a, a ' be respectively incident optical and the output optical fiber first position, by spectrum acquisition device gather incident light
The fine position a, a relative with the output optical fiber ' under transmitted spectrum and fluorescence spectrum；Displacement platform is ensureing incident optical and outgoing
On the premise of fiber-coaxial, incident optical and the output optical fiber movement is controlled to arrive position b, b respectively ' place, adopted by spectrum acquisition device
Collect incident optical and the output optical fiber relative position b, b ' under transmitted spectrum and fluorescence spectrum.
Further, the transmitted light source is super continuous wide range laser, the super continuous wide range laser covering visible light wave band
Or the combination of near infrared light wave band or both；Fluorescence excitation light source is ultraviolet lamp, and above-mentioned light source can directly light or through incidence
Fiber optic conduction.
Further, institute's translation stage is stepper motor；The spectrum acquisition device is spectrometer.
Further, the transmitted light source is xenon lamp wide spectrum light source or bromine tungsten filament lamp wide spectrum light source, covering visible light wave band or
The combination of near infrared light wave band or both；The fluorescence excitation light source is ultraviolet laser tube or ultra-violet light-emitting pipe, and above-mentioned light source can
Directly light or conducted through incident optical；Institute's translation stage is magnet attraction device.
Further, the mathematical modeling utilizes principal component analysis, artificial neural network, PLS, support
Vector machine, signal analysis or statistical method are established.
The beneficial effect of technical scheme provided by the invention is：
1st, the present invention changes light path by command displacement platform, and the bag under same transmitted light source is gathered in different light path strong points
The packed complicated solution fluorescence spectrum under complicated solution transmitted spectrum and same fluorescence excitation light source is filled, is realized accordingly to packed multiple
The Non-Destructive Testing of mixture solution component content；
2nd, the present invention makes full use of some particular matters in complicated solution to be produced the characteristic of fluorescence by ultraviolet excitation,
But different fluorescence intensities is produced due to different with ultraviolet light incidence depth on light path direction, and excites fluorescence to produce position
Putting can cause the self-absorption of fluorescence different from the distance difference of receiving position, and be stimulated in complicated solution material simultaneously by
The influence of other material concentrations, when ultraviolet light is by the more of other material absorbings, the ultraviolet light that the material that can be excited receives is got over
It is few, thus the spectrum for causing to obtain have it is very strong non-linear；
3rd, the present invention obtained transmitted spectrum of measurement and fluorescence spectrum are above-mentioned to cause the nonlinear factor of spectrum to be made jointly
Spectrum under, the information content for the material that is stimulated is added, not only eliminate the shadow that transmitted light source change is brought with bag-like container
Ring, and measurement is with strong points, greatly inhibits the non-linear effects of spectrum；
4th, the present invention improves the precision that complicated solution surveys component content analysis；Realize quick, free of contamination packed
The measurement of complicated solution composition, it is workable.
Brief description of the drawings
Fig. 1 is double light path transmitted spectrum principle schematics；
Fig. 2 is that the method for double light path transmissions and the packed complicated solution component content of fluorescence spectral measuring is illustrated in embodiment 1
Figure；
Fig. 3 is that the method for double light path transmissions and the packed complicated solution component content of fluorescence spectral measuring is another in embodiment 2
Schematic diagram；
Fig. 4 is that the method for double light path transmissions and the packed complicated solution component content of fluorescence spectral measuring is another in embodiment 3
Schematic diagram；
Fig. 5 is that the method for double light path transmissions and the packed complicated solution component content of fluorescence spectral measuring is another in embodiment 4
Schematic diagram；
Fig. 6 is that the method for double light path transmissions and the packed complicated solution component content of fluorescence spectral measuring is another in embodiment 5
Schematic diagram；
Fig. 7 is that the method for double light path transmissions and the packed complicated solution component content of fluorescence spectral measuring is another in embodiment 6
Schematic diagram；
Fig. 8 is that the method for double light path transmissions and the packed complicated solution component content of fluorescence spectral measuring is another in embodiment 7
Schematic diagram；
Fig. 9 is that the method for double light path transmissions and the packed complicated solution component content of fluorescence spectral measuring is another in embodiment 8
Schematic diagram.
In accompanying drawing, the list of parts representated by each label is as follows：
1：First light path； 2：Second light path；
3：Light source；Including transmitted light source and fluorescence excitation light source； 4：Incident optical；
5：Packaging bag； 6：Displacement platform；
7：Spectrum acquisition device； 8：The output optical fiber；
a、a’：First position； b、b’：The second place.
Embodiment
To make the object, technical solutions and advantages of the present invention clearer, embodiment of the present invention is made below further
It is described in detail on ground.
Double light path transmission spectrum methods are according to Lambert-Beer's law, as shown in figure 1, setting the first light path 1 and second respectively
Light path 2.Derivation is as follows：
Wherein, A1It is the absorbance of the first light path 1, A2It is the absorbance of the second light path 2.IOIt is the incident light of the first light path 1
Light intensity, while be also the second light path 2 incident light light intensity, I1It is the output intensity of the first light path 1, I2It is the second light path 2
Output intensity, b1It is the optical length of the first light path 1, b2It is the optical length of the second light path 2, △ b are the difference of two optical lengths, ∈ extinctions
Coefficient is that c surveys material concentration.
The absorbance of double light path spectroscopic methodologies is still linear with optical path difference it can be seen from formula (7), meet lambert-
Beer law, and with light intensity of incident light IOIt is unrelated.Therefore, double light path methods are not influenceed by light source in theory, are deducted simultaneously
The influence of packaging bag in itself.
Due to the complexity of complicated solution composition, simple transmitted spectrum specific aim is poor, packed multiple further to improve
The measurement accuracy of mixture solution component content, the characteristics of with reference to exciting fluorescence with strong points, but due on light path direction with ultraviolet
Light incidence depth is different and produces different fluorescence intensities, and excites fluorescence generation position different from the distance of receiving position
The self-absorption of fluorescence can be caused different, and the material that is stimulated in complicated solution is influenceed by other material concentrations simultaneously, works as purple
For outer light by the more of other material absorbings, the ultraviolet light that the material that can be excited receives is fewer, therefore causes the spectrum tool obtained
Have very strong non-linear.
The transmitted spectrum and fluorescence spectrum that double light path measurements obtain are above-mentioned to cause the nonlinear factor collective effect of spectrum
Under spectrum, add the information content of the material that is stimulated, not only eliminate transmitted light source change and influence that bag-like container is brought,
And measurement is with strong points, greatly inhibits the non-linear effects of spectrum, the essence that complicated solution surveys component content analysis is improved
Degree.The measurement of quick, free of contamination packed complicated solution composition is realized, it is workable.
Embodiment 1
Double light path transmissions provided in an embodiment of the present invention and the method for the packed complicated solution component content of fluorescence spectral measuring,
The device that uses as shown in Fig. 2 including：Light source 3, packaging bag 5, displacement platform 6 and spectrum acquisition device 7.
Wherein, ensure that the light extraction optical port of light source 3 is close to packaging bag 5 and coaxial, light source 3 with the entrance slit of spectrum acquisition device 7
The complicated solution in packaging bag 5 is transmitted and excited, is filled by spectrum acquisition at first position a (corresponding first light path 1) place
Put 7 acquisition of transmission spectrum and fluorescence spectrum.The light extraction optical port of light source 3 and spectrum acquisition device 7 are then being ensured by displacement platform 6
On the premise of exit slit is coaxial, control light source is moved to second place b (corresponding second light path 2), is adopted by spectrum acquisition device 7
Collect transmitted spectrum and fluorescence spectrum.
Logarithm is asked to be absorbed spectrum light intensity ratio under each wavelength of the transmitted spectrum of two opening positions of a, b collection,
The absorption spectrum is normalized with reference to two fluorescence spectrums, and method for normalizing is：
Ag=A/max (A) (8)
In formula (8), AgTo normalize absorbance, max (A) is the absorbance maximum on different wave length, and A is extinction
Degree.With the Comparative result of existing chemical analysis, utilize principal component analysis (PCA, principal component analysis)
Or artificial neural network (ANN, artificial neural network) or PLS (PLSR, particle
Least squares calibration analysis) or SVMs (SVM, support vector machines)
The methods of signal analysis or statistics, can founding mathematical models.
The embodiment of the present invention is not repeated the process of above-mentioned founding mathematical models, known to those skilled in the art.
The transmitted spectrum and fluorescence spectrum of position at unknown packed complicated solution a, b two are gathered, by the ratio of two transmitted spectrums
After value asks the absorption spectrum that logarithm obtains and two fluorescence spectrums normalization, bring the above-mentioned mathematical modeling established into and carry out
Calculate, obtain the content that complicated solution surveys target component.
To the model of each device in addition to specified otherwise is done, the model of other devices is not limited the embodiment of the present invention,
As long as the device of above-mentioned function can be completed.
In summary, the embodiments of the invention provide a kind of double light paths transmission and the packed complicated solution of fluorescence spectral measuring into
Divide the method for content, not only eliminate the influence that transmitted light source change is brought with bag-like container, and measurement is with strong points, greatly presses down
The non-linear effects of spectrum have been made, have improved the precision that complicated solution surveys component content analysis.Realize quick, free of contamination
The measurement of packed complicated solution composition, it is workable.
Embodiment 2
The embodiment of the present invention differs only in embodiment 1, and the move mode of light source 3 and spectrum acquisition device 7 is not
Together, it is described below：
Referring to Fig. 3, ensure that the light extraction optical port of light source 3 is close to packaging bag 5 and coaxial, light with the entrance slit of spectrum acquisition device 7
Source 3 is transmitted and excited to the complicated solution in packaging bag 5, by spectrum acquisition device 7 in first position a acquisition of transmission light
Spectrum and fluorescence spectrum.Then ensureing that the light extraction optical port of light source 3 and the entrance slit of spectrum acquisition device 7 are coaxial by displacement platform 6
On the premise of, control spectrum acquisition device 7 is moved to second place b, gathers transmitted spectrum and fluorescence light at the b of the second place
Spectrum.
Wherein, follow-up normalization, founding mathematical models and calculating complicated solution survey the step of the content of target component
Rapid same as Example 1, the embodiment of the present invention is not repeated this.
To the model of each device in addition to specified otherwise is done, the model of other devices is not limited the embodiment of the present invention,
As long as the device of above-mentioned function can be completed.
In summary, the embodiments of the invention provide a kind of double light paths transmission and the packed complicated solution of fluorescence spectral measuring into
Divide the method for content, not only eliminate the influence that transmitted light source change is brought with bag-like container, and measurement is with strong points, greatly presses down
The non-linear effects of spectrum have been made, have improved the precision that complicated solution surveys component content analysis.Realize quick, free of contamination
The measurement of packed complicated solution composition, it is workable.
Embodiment 3
The embodiment of the present invention differs only in embodiment 1, and the moving direction of light source 3 and spectrum acquisition device 7 is not
Together, it is described below：
Referring to Fig. 4, ensure that light source 3 is close to packaging bag 5 with spectrum acquisition device 7 and ensures the light extraction optical port of light source 3 and spectrum
The entrance slit of reception device 7 is coaxial, and the complicated solution in packaging bag 5 is transmitted and swashed by light source 3 at a of first position
Hair, at first position a ' places by the acquisition of transmission spectrum of spectrum acquisition device 7 and fluorescence spectrum, is then being protected by displacement platform 6
On the premise of the card light extraction optical port of light source 3 and the entrance slit of spectrum acquisition device 7 are coaxial, light source 3 and spectrum acquisition device 7 is controlled to divide
The second place b, b are not moved to ' place, gather the transmitted spectrum and fluorescence spectrum of the opening position.
Wherein, follow-up normalization, founding mathematical models and calculating complicated solution survey the step of the content of target component
Rapid same as Example 1, the embodiment of the present invention is not repeated this.
To the model of each device in addition to specified otherwise is done, the model of other devices is not limited the embodiment of the present invention,
As long as the device of above-mentioned function can be completed.
In summary, the embodiments of the invention provide a kind of double light paths transmission and the packed complicated solution of fluorescence spectral measuring into
Divide the method for content, not only eliminate the influence that transmitted light source change is brought with bag-like container, and measurement is with strong points, greatly presses down
The non-linear effects of spectrum have been made, have improved the precision that complicated solution surveys component content analysis.Realize quick, free of contamination
The measurement of packed complicated solution composition, it is workable.
Embodiment 4
During specific implementation, due to the limitation of space structure, it is possible that light source 3 can not be close to spectrum acquisition device 7
The situation of packaging bag 5, at this moment can be by setting an optical fiber, as incident optical respectively at light source 3 and spectrum acquisition device 7
4 with the output optical fiber 8.
Referring to Fig. 5, light source 3 is transmitted and excited to the complicated solution in packaging bag 5 by incident optical 4, by spectrum
Reception device 7 passes through the acquisition of transmission spectrum of the output optical fiber 8 and fluorescence spectrum, and incident optical 4 is close to pack respectively with the output optical fiber 8
Bag 5 and ensure coaxial, a positions are the first position of incident optical 4, and spectrum acquisition device 7 gathers incident optical 4 and the output optical fiber
Transmitted spectrum and fluorescence spectrum under 8 relative first position a；Incident optical 4 and outgoing are then being ensured by displacement platform 6
On the premise of the position of optical fiber 8 is still coaxial, control incident optical 4 is moved at the b of the second place, spectrum acquisition device 5 gather into
The transmitted spectrum and fluorescence spectrum penetrated under the optical fiber 4 and relative second position b of the output optical fiber 8.
Wherein, follow-up normalization, founding mathematical models and calculating complicated solution survey the step of the content of target component
Rapid same as Example 1, the embodiment of the present invention is not repeated this.
To the model of each device in addition to specified otherwise is done, the model of other devices is not limited the embodiment of the present invention,
As long as the device of above-mentioned function can be completed.
In summary, the embodiments of the invention provide a kind of double light paths transmission and the packed complicated solution of fluorescence spectral measuring into
Divide the method for content, not only eliminate the influence that transmitted light source change is brought with bag-like container, and measurement is with strong points, greatly presses down
The non-linear effects of spectrum have been made, have improved the precision that complicated solution surveys component content analysis.Realize quick, free of contamination
The measurement of packed complicated solution composition, it is workable.
Embodiment 5
The embodiment of the present invention is different only in that with embodiment 4, the output optical fiber 8, sets with first position a, second place b
Difference is put, it is described below：
Referring to Fig. 6, light source 3 is transmitted and excited to the complicated solution in packaging bag 5 by incident optical 4, by spectrum
Reception device 7 passes through the acquisition of transmission spectrum of the output optical fiber 8 and fluorescence spectrum, and incident optical 4 is close to pack respectively with the output optical fiber 8
Bag 5 and ensure coaxial, a positions are the first position of the output optical fiber 8, and spectrum acquisition device 7 gathers incident optical 4 and the output optical fiber
Transmitted spectrum and fluorescence spectrum under 8 relative first position a；Incident optical 4 and outgoing are then being ensured by displacement platform 6
On the premise of the position of optical fiber 8 is still coaxial, control the output optical fiber 8 is moved at the b of the second place, spectrum acquisition device 5 gather into
The transmitted spectrum and fluorescence spectrum penetrated under the optical fiber 4 and relative second position b of the output optical fiber 8.
Wherein, follow-up normalization, founding mathematical models and calculating complicated solution survey the step of the content of target component
Rapid same as Example 1, the embodiment of the present invention is not repeated this.
To the model of each device in addition to specified otherwise is done, the model of other devices is not limited the embodiment of the present invention,
As long as the device of above-mentioned function can be completed.
In summary, the embodiments of the invention provide a kind of double light paths transmission and the packed complicated solution of fluorescence spectral measuring into
Divide the method for content, not only eliminate the influence that transmitted light source change is brought with bag-like container, and measurement is with strong points, greatly presses down
The non-linear effects of spectrum have been made, have improved the precision that complicated solution surveys component content analysis.Realize quick, free of contamination
The measurement of packed complicated solution composition, it is workable.
Embodiment 6
The embodiment of the present invention is different only in that with embodiment 4, incident optical 4, the output optical fiber 8, with first position a, the
Two position b setting is different, described below：
Referring to Fig. 7, light source 3 is transmitted and excited to the complicated solution in packaging bag 5 by incident optical 4, by spectrum
Reception device 7 passes through the acquisition of transmission spectrum of the output optical fiber 8 and fluorescence spectrum, and incident optical 4 is close to pack respectively with the output optical fiber 8
Bag 5 and ensure coaxial, a, a ' be respectively incident optical 4 and the output optical fiber 8 first position, spectrum acquisition device 7 gathers incidence
Transmitted spectrum and fluorescence spectrum of the optical fiber 4 with the output optical fiber 8 under relative first position a, a '；Then existed by displacement platform 6
Ensure incident optical 4 and the position of the output optical fiber 8 remain unchanged it is coaxial on the premise of, control incident optical 4 and the output optical fiber 8 move respectively
To the second place b, b ' place, the collection incident optical 4 of spectrum acquisition device 7 is with the output optical fiber 8 with respect to the second place b, b ' under
Transmitted spectrum and fluorescence spectrum.
Wherein, follow-up normalization, founding mathematical models and calculating complicated solution survey the step of the content of target component
Rapid same as Example 1, the embodiment of the present invention is not repeated this.
To the model of each device in addition to specified otherwise is done, the model of other devices is not limited the embodiment of the present invention,
As long as the device of above-mentioned function can be completed.
In summary, the embodiments of the invention provide a kind of double light paths transmission and the packed complicated solution of fluorescence spectral measuring into
Divide the method for content, not only eliminate the influence that transmitted light source change is brought with bag-like container, and measurement is with strong points, greatly presses down
The non-linear effects of spectrum have been made, have improved the precision that complicated solution surveys component content analysis.Realize quick, free of contamination
The measurement of packed complicated solution composition, it is workable.
Embodiment 7
As different from Example 4, the embodiment only includes incident optical 4 to the embodiment of the present invention, described below：
Referring to Fig. 8, light source 3 is transmitted and excited to the complicated solution in packaging bag 5 by incident optical 4, by spectrum
The acquisition of transmission spectrum of reception device 7 and fluorescence spectrum, incident optical 4 are close to pack respectively with the entrance slit of spectrum acquisition device 7
Bag 5 and ensure coaxial, a positions are the first position of incident optical 4, spectrum acquisition device 7 gather incident optical 4 with respect to this first
Transmitted spectrum and fluorescence spectrum under a of position；Then ensureing that incident optical 4 and spectrum acquisition device 7 are narrow by displacement platform 6
On the premise of seam position is still coaxial, control incident optical 4 is moved at the b of the second place, and spectrum acquisition device 7 gathers incident light
Transmitted spectrum and fluorescence spectrum under 4 relative second position b of fibre.
Wherein, follow-up normalization, founding mathematical models and calculating complicated solution survey the step of the content of target component
Rapid same as Example 1, the embodiment of the present invention is not repeated this.
To the model of each device in addition to specified otherwise is done, the model of other devices is not limited the embodiment of the present invention,
As long as the device of above-mentioned function can be completed.
In summary, the embodiments of the invention provide a kind of double light paths transmission and the packed complicated solution of fluorescence spectral measuring into
Divide the method for content, not only eliminate the influence that transmitted light source change is brought with bag-like container, and measurement is with strong points, greatly presses down
The non-linear effects of spectrum have been made, have improved the precision that complicated solution surveys component content analysis.Realize quick, free of contamination
The measurement of packed complicated solution composition, it is workable.
Embodiment 8
As different from Example 4, the embodiment only includes the output optical fiber 8 to the embodiment of the present invention, described below：
Referring to Fig. 9, light source 3 is transmitted and excited to the complicated solution in packaging bag 5, is passed through by spectrum acquisition device 7
The acquisition of transmission spectrum of the output optical fiber 8 and fluorescence spectrum, the light extraction optical port of light source 3 are close to packaging bag 5 with the output optical fiber 8 and ensured respectively
Coaxially, spectrum acquisition device 7 gathers the transmitted spectrum and fluorescence spectrum under the light source 3 and relative first position a of the output optical fiber 8；With
Afterwards by displacement platform 6 ensure the light extraction optical port of light source 3 and the position of the output optical fiber 8 remain unchanged it is coaxial on the premise of, control light source 3 to move
Move at the b of the second place, spectrum acquisition device 5 gather transmitted spectrum under light source 3 and the relative second position b of the output optical fiber 8 and
Fluorescence spectrum.
Wherein, follow-up normalization, founding mathematical models and calculating complicated solution survey the step of the content of target component
Rapid same as Example 1, the embodiment of the present invention is not repeated this.
To the model of each device in addition to specified otherwise is done, the model of other devices is not limited the embodiment of the present invention,
As long as the device of above-mentioned function can be completed.
In summary, the embodiments of the invention provide a kind of double light paths transmission and the packed complicated solution of fluorescence spectral measuring into
Divide the method for content, not only eliminate the influence that transmitted light source change is brought with bag-like container, and measurement is with strong points, greatly presses down
The non-linear effects of spectrum have been made, have improved the precision that complicated solution surveys component content analysis.Realize quick, free of contamination
The measurement of packed complicated solution composition, it is workable.
Embodiment 9
Select, the scheme in above-described embodiment 1-6 is further introduced, light source 3 with reference to specific device：
Including transmitted light source and fluorescence excitation light source, transmitted light source can be super continuous wide range laser, the super continuous wide range laser covering
The combination of visible light wave range or near infrared light wave band or both, excitation source is ultraviolet lamp, above-mentioned light source can directly light or
Conducted through incident optical 4.Displacement platform 6 is stepper motor, and spectrum acquisition device 7 is spectrometer, described below：
As shown in figure 5, above-mentioned light source 3 is transmitted and excited to the complicated solution in packaging bag 5 by incident optical 4,
Spectrometer 7 passes through the acquisition of transmission spectrum of the output optical fiber 8 and fluorescence spectrum, and incident optical 4 is close to packaging bag respectively with the output optical fiber 8
5 and coaxial, a positions are the first position of incident optical 4, spectrometer 7 gather incident optical 4 and the output optical fiber 8 with respect to this first
Transmitted spectrum and fluorescence spectrum under a of position；Then by stepper motor 6 ensure incident optical 4 and the position of the output optical fiber 8 according to
It is old it is coaxial on the premise of, control incident optical 4 is moved to the b of the second place at, and spectrometer 7 gathers incident optical 4 and the output optical fiber 8
Transmitted spectrum and fluorescence spectrum under relative second position b.
Wherein, follow-up normalization, founding mathematical models and calculating complicated solution survey the step of the content of target component
Rapid same as Example 1, the embodiment of the present invention is not repeated this.
To the model of each device in addition to specified otherwise is done, the model of other devices is not limited the embodiment of the present invention,
As long as the device of above-mentioned function can be completed.
In summary, the embodiments of the invention provide a kind of double light paths transmission and the packed complicated solution of fluorescence spectral measuring into
Divide the method for content, not only eliminate the influence that transmitted light source change is brought with bag-like container, and measurement is with strong points, greatly presses down
The non-linear effects of spectrum have been made, have improved the precision that complicated solution surveys component content analysis.Realize quick, free of contamination
The measurement of packed complicated solution composition, it is workable.
Embodiment 10
As different from Example 9, transmitted light source is bromine tungsten filament lamp wide spectrum light source to the embodiment of the present invention, the bromine tungsten filament lamp wide spectrum optical
The combination of source covering visible light wave band or near infrared light wave band or both, fluorescence excitation light source is ultraviolet laser tube, above-mentioned light source 3
It can directly light or be conducted through incident optical 4.
As shown in figure 5, above-mentioned light source 3 is transmitted and excited to the complicated solution in packaging bag 5 by incident optical 4,
The acquisition of transmission spectrum of the output optical fiber 8 and fluorescence spectrum are passed through by spectrometer 7, incident optical 4 is close to pack respectively with the output optical fiber 8
Bag 5 and coaxial, transmitted spectrum and fluorescence light of the collection incident optical 4 of spectrometer 7 with the output optical fiber 8 under relative first position a
Spectrum；Then by stepper motor 6 ensure incident optical 4 and the position of the output optical fiber 8 remain unchanged it is coaxial on the premise of, control incident light
Fibre 4 is moved at the b of the second place, and spectrometer 7 gathers the transmitted spectrum under the incident optical 4 and relative second position b of the output optical fiber 8
And fluorescence spectrum.
Wherein, follow-up normalization, founding mathematical models and calculating complicated solution survey the step of the content of target component
Rapid same as Example 1, the embodiment of the present invention is not repeated this.
To the model of each device in addition to specified otherwise is done, the model of other devices is not limited the embodiment of the present invention,
As long as the device of above-mentioned function can be completed.
In summary, the embodiments of the invention provide a kind of double light paths transmission and the packed complicated solution of fluorescence spectral measuring into
Divide the method for content, not only eliminate the influence that transmitted light source change is brought with bag-like container, and measurement is with strong points, greatly presses down
The non-linear effects of spectrum have been made, have improved the precision that complicated solution surveys component content analysis.Realize quick, free of contamination
The measurement of packed complicated solution composition, it is workable.
Embodiment 11
The embodiment of the present invention is unlike embodiment 9,10, and transmitted light source is xenon lamp wide spectrum light source, the xenon lamp wide spectrum optical
The combination of source covering visible light wave band or near infrared light wave band or both, fluorescence excitation light source are ultra-violet light-emitting pipe, above-mentioned light source 3
It can directly light or be conducted through incident optical 4.Displacement platform 6 is magnet attraction device, described below：
As shown in figure 5, above-mentioned light source 3 is transmitted and excited to the complicated solution in packaging bag 5 by incident optical 4,
Spectrometer 7 passes through the acquisition of transmission spectrum of the output optical fiber 8 and fluorescence spectrum, and incident optical 4 is close to packaging bag respectively with the output optical fiber 8
5 and coaxial, spectrometer 7 gathers incident optical 4 with the output optical fiber 8 with respect to the transmitted spectrum and fluorescence spectrum under a of the first position；
Then by magnet attraction device 6 ensure incident optical 4 and the position of the output optical fiber 8 remain unchanged it is coaxial on the premise of, control incidence
Optical fiber 4 is moved at the b of the second place, and spectrometer 7 gathers the transmitted light under the incident optical 4 and relative second position b of the output optical fiber 8
Spectrum and fluorescence spectrum.
Wherein, follow-up normalization, founding mathematical models and calculating complicated solution survey the step of the content of target component
Rapid same as Example 1, the embodiment of the present invention is not repeated this.
To the model of each device in addition to specified otherwise is done, the model of other devices is not limited the embodiment of the present invention,
As long as the device of above-mentioned function can be completed.
In summary, the embodiments of the invention provide a kind of double light paths transmission and the packed complicated solution of fluorescence spectral measuring into
Divide the method for content, not only eliminate the influence that transmitted light source change is brought with bag-like container, and measurement is with strong points, greatly presses down
The non-linear effects of spectrum have been made, have improved the precision that complicated solution surveys component content analysis.Realize quick, free of contamination
The measurement of packed complicated solution composition, it is workable.
Embodiment 12
The embodiment of the present invention unlike above-described embodiment 9,10,11, may be used also by needs of the light source 3 in practical application
Can also use other mobile devices using light source, the displacement platform 6 of other models, spectrum acquisition device 7 can also be adopted
With other reception devices.During specific implementation, the embodiment of the present invention is not limited to the model of above-mentioned device.
The embodiment of the present invention is to position a, a '；Position b, b ' and move mode etc. be not restricted, as long as this hair can be realized
The function of bright embodiment, within the protection domain of the application.
In summary, the embodiments of the invention provide a kind of double light paths transmission and the packed complicated solution of fluorescence spectral measuring into
Divide the method for content, not only eliminate the influence that transmitted light source change is brought with bag-like container, and measurement is with strong points, greatly presses down
The non-linear effects of spectrum have been made, have improved the precision that complicated solution surveys component content analysis.Realize quick, free of contamination
The measurement of packed complicated solution composition, it is workable.
Claims (10)
1. a kind of double light path transmissions and the method for the packed complicated solution component content of fluorescence spectral measuring, it is characterised in that described
Method comprises the following steps：
The light extraction optical port of light source is close to packaging bag and coaxial with the entrance slit of spectrum acquisition device, light source include transmitted light source and
Fluorescence excitation light source, transmitted light source transmit to the complicated solution in packaging bag, the production of fluorescent exciting source excitation complicated solution
Raw fluorescence, spectrum acquisition device acquisition of transmission spectrum and fluorescence spectrum；
Displacement platform is before ensureing that transmitted light source and fluorescence excitation light source light extraction optical port and spectrum acquisition device entrance slit are coaxial
Put, transmitted light source and fluorescence excitation light source movement are controlled, by spectrum acquisition device acquisition of transmission spectrum and fluorescence spectrum；
Ask logarithm to be absorbed spectrum light intensity ratio under each wavelength of two opening position transmitted spectrums, and combine two positions
Under fluorescence spectrum normalized, the Comparative result with existing chemical analysis, founding mathematical models；
The transmitted spectrum and fluorescence spectrum at unknown packed complicated solution two positions are gathered, the ratio of two transmitted spectrums is sought into logarithm
Obtained absorption spectrum and two fluorescence spectrums are brought mathematical modeling into after normalizing and calculated, and obtain complicated solution and are surveyed
The content of target component；
The transmitted spectrum and fluorescence spectrum are to cause the spectrum under the nonlinear factor collective effect of spectrum, and stimulating food is excited in increase
The information content of matter；Eliminate the influence that transmitted light source change is brought with bag-like container；Suppress the non-linear effects of spectrum；Improve complicated
Solution surveys the precision of component content analysis.
2. a kind of double light path transmissions according to claim 1 and the side of the packed complicated solution component content of fluorescence spectral measuring
Method, it is characterised in that displacement platform control light source movement, the step of by spectrum acquisition device acquisition of transmission spectrum and fluorescence spectrum
Specially：
The complicated solution in packaging bag is transmitted and excited respectively by transmitted light source and fluorescence excitation light source at a of position,
By spectrum acquisition device acquisition of transmission spectrum and fluorescence spectrum；
Displacement platform control light source is moved to position b, by spectrum acquisition device acquisition of transmission spectrum and fluorescence spectrum；
Or,
Transmitted light source and fluorescence excitation light source are transmitted and excited respectively to the complicated solution in packaging bag, are filled by spectrum acquisition
Put acquisition of transmission spectrum and fluorescence spectrum at a of position；
Displacement platform control spectrum acquisition device is moved to position b, gathers transmitted spectrum and fluorescence spectrum at the b of position；
Or,
The complicated solution in packaging bag is transmitted and excited respectively by transmitted light source and fluorescence excitation light source at a of position,
At position a ' places by spectrum acquisition device acquisition of transmission spectrum and fluorescence spectrum；
Displacement platform controls transmitted light source and fluorescence excitation light source to be moved to position b, and spectrum acquisition device is moved to position b ' places,
By spectrum acquisition device acquisition of transmission spectrum and fluorescence spectrum.
3. a kind of double light path transmissions according to claim 1 or 2 and the packed complicated solution component content of fluorescence spectral measuring
Method, it is characterised in that methods described also includes：
One optical fiber is set at light source, as incident optical, and ensures that incident optical is close to spectrum acquisition device entrance slit
Packaging bag and coaxial；
Or,
One optical fiber is set at spectrum acquisition device, as the output optical fiber, and ensures that the output optical fiber is close to light source light extraction optical port
Packaging bag and coaxial；
Or,
Incident optical and the output optical fiber are set respectively at light source and spectrum acquisition device, and ensure incident optical and the output optical fiber
It is close to packaging bag and coaxial.
4. a kind of double light path transmissions according to claim 3 and the side of the packed complicated solution component content of fluorescence spectral measuring
Method, it is characterised in that
A positions are the first position of incident optical, are gathered by spectrum acquisition device under incident optical and the output optical fiber relative position a
Transmitted spectrum and fluorescence spectrum；Displacement platform is on the premise of ensureing that incident optical and the output optical fiber are coaxial, control incident light
Fibre is moved at the b of position, and incident optical and the transmitted spectrum under the output optical fiber relative position b and glimmering are gathered by spectrum acquisition device
Light spectrum.
5. a kind of double light path transmissions according to claim 3 and the side of the packed complicated solution component content of fluorescence spectral measuring
Method, it is characterised in that
A positions are the first position of the output optical fiber, are gathered by spectrum acquisition device under incident optical and the output optical fiber relative position a
Transmitted spectrum and fluorescence spectrum；Displacement platform is on the premise of ensureing that incident optical and the output optical fiber are coaxial, control emergent light
Fibre is moved at the b of position, and incident optical and the transmitted spectrum under the output optical fiber relative position b and glimmering are gathered by spectrum acquisition device
Light spectrum.
6. a kind of double light path transmissions according to claim 3 and the side of the packed complicated solution component content of fluorescence spectral measuring
Method, it is characterised in that
A, a ' is respectively the first position of incident optical and the output optical fiber, and incident optical and emergent light are gathered by spectrum acquisition device
Transmitted spectrum and fluorescence spectrum under relative position a, a ' of fibre；Displacement platform is ensureing that incident optical and the output optical fiber are coaxial
Under the premise of, control incident optical and the output optical fiber movement to arrive position b, b respectively ' place, incident optical is gathered by spectrum acquisition device
With the output optical fiber relative position b, b ' under transmitted spectrum and fluorescence spectrum.
7. a kind of double light path transmissions according to claim 3 and the side of the packed complicated solution component content of fluorescence spectral measuring
Method, it is characterised in that the transmitted light source is super continuous wide range laser, the super continuous wide range laser covering visible light wave band or near
The combination of infrared band or both；Fluorescence excitation light source is ultraviolet lamp, and above-mentioned light source can directly light or through incident optical
Conduction.
8. a kind of double light path transmissions according to claim 3 and the side of the packed complicated solution component content of fluorescence spectral measuring
Method, it is characterised in that institute's translation stage is stepper motor；The spectrum acquisition device is spectrometer.
9. a kind of double light path transmissions according to claim 3 and the side of the packed complicated solution component content of fluorescence spectral measuring
Method, it is characterised in that the transmitted light source is xenon lamp wide spectrum light source or bromine tungsten filament lamp wide spectrum light source, covering visible light wave band or near red
The combination of outer optical band or both；The fluorescence excitation light source is ultraviolet laser tube or ultra-violet light-emitting pipe, and above-mentioned light source can be direct
Light or conducted through incident optical；Institute's translation stage is magnet attraction device.
10. a kind of double light path transmissions and fluorescence spectral measuring according to any claim in claim 1-3 are packed multiple
The method of mixture solution component content, it is characterised in that
The mathematical modeling utilizes principal component analysis, artificial neural network, PLS, SVMs, signal point
Analysis or statistical method are established.
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| CN101813623A (en) * | 2010-04-22 | 2010-08-25 | 天津大学 | Scatterer multi-dimensional spectral measuring unit and method |
| CN102564983A (en) * | 2012-02-14 | 2012-07-11 | 天津大学 | Optical detection device and method for concentration of components in turbid medium based on limited range wavelength method |
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Application publication date: 20171201 |