CN107356533A - The method of free hemoglobin content in multiposition modulated light source measurement blood bag - Google Patents
The method of free hemoglobin content in multiposition modulated light source measurement blood bag Download PDFInfo
- Publication number
- CN107356533A CN107356533A CN201710529561.4A CN201710529561A CN107356533A CN 107356533 A CN107356533 A CN 107356533A CN 201710529561 A CN201710529561 A CN 201710529561A CN 107356533 A CN107356533 A CN 107356533A
- Authority
- CN
- China
- Prior art keywords
- spectrum
- light source
- acquisition device
- blood bag
- transmitted
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/255—Details, e.g. use of specially adapted sources, lighting or optical systems
Abstract
The invention discloses the method for free hemoglobin content in a kind of multiposition modulated light source measurement blood bag, comprise the following steps：Modulating device is modulated to light source, and the light extraction optical port of light source is close to blood bag with the entrance slit of spectrum acquisition device, and displacement platform control light source is moved to multiple positions and blood sample is transmitted, by spectrum acquisition device acquisition of transmission spectrum；The time series of each wavelength in the transmitted spectrum collected is transformed into frequency domain, the transmitted spectrum in frequency domain is constructed with the fundametal compoment of each wavelength, by its normalized, the Comparative result with existing chemical analysis, founding mathematical models；Transmitted spectrum in the frequency domain of the multiple opening positions of unknown blood sample is in kind gathered, is normalized and brings mathematical modeling into, obtain the content of free hemoglobin.This invention removes spectral background noise and the influence of scattering, adds the information content of all the components in blood, improves the precision of free hemoglobin content analysis.
Description
Technical field
The present invention relates to spectrum complicated solution concentration analysis stoichiometry field, more particularly to a kind of multiposition modulated light source
The method for measuring free hemoglobin content in blood bag.
Background technology
In existing technology, more ripe technology is that free hemoglobin in blood bag is detected by way of chemical analysis
Content, there are the high outstanding advantages of accuracy, but the mode of chemical analysis needs to open blood bag and takes out sample and chemically examined, nothing
Method meets quick, non-contact, free of contamination demand.
Spectral detection is also possible to realize the inspection to free hemoglobin in blood bag due to its non-contact, free of contamination characteristic
Survey.Due to the complexity of blood constituent, therefore there is strong scattering, its transmitted spectrum is nonlinear, and traditional transmitted light
The influence of spectral background noise can not be completely eliminated in spectrum, and for this problem, method proposes a kind of multiposition modulated light source
The method for measuring free hemoglobin content in blood bag.
The content of the invention
The invention provides the method for free hemoglobin content in a kind of multiposition modulated light source measurement blood bag, the present invention
The influence for greatly inhibiting blood scattered band, improves the analysis precision of free hemoglobin concentration, described below：
The method of free hemoglobin content, methods described include following step in a kind of multiposition modulated light source measurement blood bag
Suddenly：
The light extraction optical port of light source is close to blood bag with the entrance slit of spectrum acquisition device, and modulating device modulated light source makes its hair
Go out square wave optical signal, light source transmits to blood sample, spectrum acquisition device acquisition of transmission spectrum；
Displacement platform control light source is moved to multiple positions, by spectrum acquisition device acquisition of transmission spectrum；
The transmitted spectrum that multiple opening positions collect is transformed into frequency domain, constructed at transmitted spectrum and the normalization in frequency domain
Reason, the Comparative result with existing chemical analysis, founding mathematical models；
Transmitted spectrum of the unknown blood sample in multiple opening positions is gathered, constructs in frequency domain and is brought into after transmitted spectrum normalization
Mathematical modeling, obtain the content of free hemoglobin；
Methods described changes position by command displacement platform, the blood in blood bag under collection same light source at diverse location
Transmitted spectrum；Solves the Non-Destructive Testing of free hemoglobin in blood bag；
Multiple station acquisitions to transmitted spectrum increase blood in all the components information content, suppress what blood scattered band came
Influence, eliminate spectral background noise, improve the analysis precision of free hemoglobin concentration in blood bag.
The step of transmitted spectrum in the construction frequency domain is specially：
Modulating device by modulation of source into square wave optical signal, by spectrum acquisition device acquisition of transmission spectrum, by each wavelength
Time series transform to frequency domain, with the transmitted spectrum in the fundametal compoment of each wavelength construction frequency domain.
Wherein, displacement platform control light source is moved to diverse location, the step of by spectrum acquisition device acquisition of transmission spectrum
Specially：
Light source transmits at a of position to blood sample, by spectrum acquisition device acquisition of transmission spectrum；
Displacement platform control light source is moved to position b, by spectrum acquisition device acquisition of transmission spectrum；
Displacement platform control light source is moved to position n always, by spectrum acquisition device acquisition of transmission spectrum；
Or,
Light source transmits to the blood sample in blood bag, by spectrum acquisition device in position a acquisition of transmission spectrum；
Displacement platform control spectrum acquisition device is moved to position b, gathers the transmitted spectrum at the b of position；
Displacement platform control spectrum acquisition device is moved to position n, gathers the transmitted spectrum at the n of position.
Wherein, methods described also includes：
One optical fiber is set at light source, as incident optical, and ensures the incident narrow of incident optical and spectrum acquisition device
Seam is close to blood bag；
Or,
One optical fiber is set at spectrum acquisition device, as the output optical fiber, and ensures the output optical fiber and light source light extraction optical port
It is close to blood bag；
Or,
Incident optical and the output optical fiber are set respectively at light source and spectrum acquisition device, and ensure incident optical and outgoing
Optical fiber is close to blood bag.
Wherein, a positions are the first position of incident optical, and spectrum acquisition device gathers the transmitted light under the position
Spectrum；Control incident optical is moved at the b of position, and spectrum acquisition device gathers the transmitted spectrum under the position；Control incident optical
It is moved to always at the n of position, spectrum acquisition device gathers the transmitted spectrum under the position.
Wherein, a positions are the first position of the output optical fiber, and spectrum acquisition device gathers the transmitted light under the position
Spectrum；Control the output optical fiber is moved at the b of position, and spectrum acquisition device gathers the transmitted spectrum under the position；Control the output optical fiber
It is moved to always at the n of position, spectrum acquisition device gathers the transmitted spectrum under the position.
Further, the light source is super continuous wide range laser, the super continuous wide range laser covering visible light wave band or near
The combination of infrared band or both, it can directly send transmitted light or be conducted through incident optical.
Further, institute's translation stage is stepper motor；The spectrum acquisition device is spectrometer；The modulating device
For chopper.
Further, the light source is xenon lamp wide spectrum light source or the bromine tungsten filament lamp wide spectrum light source xenon lamp wide spectrum light source or bromine tungsten filament lamp
The combination of wide spectrum light source covering visible light wave band or near infrared light wave band or both, can directly send transmitted light or through incident light
Fibre conduction.
Mathematical modeling described above utilizes principal component analysis, artificial neural network, PLS, supporting vector
Machine, signal analysis or statistical method are established.
The beneficial effect of technical scheme provided by the invention is：
1. the present invention changes position by command displacement platform, blood in the blood bag under same light source is gathered at diverse location
Liquid sample transmission spectrum；
2. the present invention solves the problems, such as the Non-Destructive Testing of free hemoglobin in blood bag, efficient, easy, pollution-free；
3. all the components scattering is influenceed in by blood, therefore spectrum can scatter in all directions, and multiple position measurements
Obtained transmitted spectrum adds the information content of all the components in blood, the influence for greatly inhibiting blood scattered band, and frequency
Transmitted spectrum eliminates the influence of spectral background noise in domain, improves the analysis precision for hemoglobin concentration of dissociating in blood bag.
Brief description of the drawings
Fig. 1 is the schematic diagram that multiposition modulated light source measures the method for free hemoglobin content in blood bag；
Fig. 2 is the method schematic diagram that multiposition modulated light source measures free hemoglobin content in blood bag in embodiment 1；
Fig. 3 is another schematic diagram of method that multiposition modulated light source measures free hemoglobin content in blood bag in embodiment 2；
Fig. 4 is another schematic diagram of method that multiposition modulated light source measures free hemoglobin content in blood bag in embodiment 3；
Fig. 5 is another schematic diagram of method that multiposition modulated light source measures free hemoglobin content in blood bag in embodiment 4；
Fig. 6 is another schematic diagram of method that multiposition modulated light source measures free hemoglobin content in blood bag in embodiment 5；
Fig. 7 is another schematic diagram of method that multiposition modulated light source measures free hemoglobin content in blood bag in embodiment 6.
In accompanying drawing, the list of parts representated by each label is as follows：
1：Light source； 2：Incident optical；
3：Blood bag； 4：Displacement platform；
5：Spectrum acquisition device； 6：The output optical fiber；
7：Modulating device； a：First position；
b：The second place； n：N-th position.
Embodiment
To make the object, technical solutions and advantages of the present invention clearer, embodiment of the present invention is made below further
It is described in detail on ground.
When modulating device is positioned among light source optical path, light can periodically by with block.Now light source is sent
Light is modulated into the square wave optical signal with certain frequency.The side sent through the transmission light frequency that complicated solution obtains with light source
Light frequency is consistent, and by taking λ wavelength as an example, Fig. 1 is the integrated value signal corresponding with the spectrum acquisition device time of integration of λ wavelength waveforms
Figure, B are ambient noise, and T is the time of integration of spectrum acquisition device, and T1=T2=...=Ti=T；T1The time of integration in section
Corresponding is ambient noise, and the light intensity amplitude that now spectrum acquisition device receives is the integrated value of ambient noise, and numerical value is minimum
It is designated as Imin；TiIt is λ wavelength light intensity and ambient noise corresponding to the time of integration in section, now spectrum acquisition device receives
Light intensity amplitude is λ wavelength light intensity and the integrated value of ambient noise, and numerical value maximum is designated as Imax, T1With TiBetween other times of integration
The corresponding ambient noise of a part, another part corresponds to λ wavelength light intensity and ambient noise, therefore resulting integrated value is in (Imin,
Imax) change in section.Thus, in T1~TiThe interior class value domain that can obtain is (Imin, Imax) integration value sequence, it can be seen that,
The integrated value of the wavelength is in (Imin, Imax) change in section and form cyclical signal, the transmitted light integrated value of other wavelength with
This is similar, and for strictly with cycle and synchronous cyclical signal.Carried out by the time series of the integrated value to each wavelength
Fourier transform, transmitted spectrum in the frequency domain formed with the frequency domain fundametal compoment of all wavelengths integrated value, the spectrum back of the body can be eliminated
Scape noise, increases substantially signal to noise ratio.
The present invention solves the influence that spectral background noise can not be completely eliminated in traditional transmitted spectrum, is dissipated because blood has
Penetrating property, therefore spectrum can scatter in all directions, and the transmitted spectrum that multiple position measurements obtain is added in blood and owned
The information content of composition, the influence for greatly inhibiting blood scattered band, improve the analysis for hemoglobin concentration of dissociating in blood bag
Precision.
Embodiment 1
The method of free hemoglobin content, is made in multiposition modulated light source measurement blood bag provided in an embodiment of the present invention
The device used as shown in Fig. 2 including：Light source 1, blood bag 3, displacement platform 4, spectrum acquisition device 5 and modulating device 7.
Wherein, ensure that the light extraction optical port of light source 1 is close to blood bag 3, modulating device 7 with the entrance slit of spectrum acquisition device 5
Modulated light source 1 makes its sender's wave optical signal, and light source 1 transmits at a of first position to the blood sample in blood bag 3, by
The acquisition of transmission spectrum of spectrum acquisition device 5.Then light source 1 is controlled to be moved to second place b, spectrum acquisition by displacement platform 4
Device 5 gathers the transmitted spectrum of the opening position, controls light source 1 to be moved to always at the n of position by displacement platform 4, spectrum acquisition
Device 5 gathers the transmitted spectrum under the position.
The time series of each wavelength of the transmitted spectrum of the how individual opening position collections of a, b ... n is transformed into frequency domain, with each
Transmitted spectrum and normalized, method for normalizing in the fundametal compoment construction frequency domain of wavelength are：
Ag=A/max (A) (1)
In formula (1), AgTo normalize absorbance, max (A) is the absorbance maximum on different wave length, and A is extinction
Degree.With reference to the data of chemical analysis, principal component analysis (PCA, principal component analysis) or artificial is utilized
Neutral net (ANN, artificial neural network) or PLS (PLSR, particle least
Squares calibration analysis) or SVMs (SVM, support vector machines) signal point
The methods of analysis or statistics, can founding mathematical models.
The step of embodiment of the present invention is to specific founding mathematical models do not repeat, known to those skilled in the art.
The transmitted spectrum of the how individual positions of unknown blood sample a, b ... n is gathered, is normalized after constructing the transmitted spectrum in frequency domain
Bring the above-mentioned mathematical modeling established into, obtain the content of free hemoglobin.
To the model of each device in addition to specified otherwise is done, the model of other devices is not limited the embodiment of the present invention,
As long as the device of above-mentioned function can be completed.
In summary, the embodiments of the invention provide free hemoglobin in a kind of multiposition modulated light source measurement blood bag to contain
The method of amount, solve the problems, such as the Non-Destructive Testing of free hemoglobin in blood bag, it is efficient, easy, pollution-free；Eliminate the spectrum back of the body
The influence of scape noise；The transmitted spectrum that multiposition measuring obtains adds the information content of all the components in blood, greatly suppresses
The influence that blood scattered band comes, improve the analysis precision of free hemoglobin concentration in blood bag.
Embodiment 2
The embodiment of the present invention differs only in embodiment 1, and the move mode of light source 1 and spectrum acquisition device 5 is not
Together, it is described below：
Referring to Fig. 3, ensure that the light extraction optical port of light source 1 is close to blood bag 3, modulation dress with the entrance slit of spectrum acquisition device 5
Putting 7 modulated light sources 1 makes its sender's wave optical signal, and light source 1 is transmitted to the blood sample in blood bag 3, filled by spectrum acquisition
Put 5 at a of first position acquisition of transmission spectrum.Then spectrum acquisition device 5 is controlled to be moved to the second place by displacement platform 4
B, gather the transmitted spectrum at the b of the second place；Spectrum acquisition device 5 is controlled to be moved to always at the n of position by displacement platform 4,
Gather the transmitted spectrum at the n of position.
Wherein, the transmitted spectrum in follow-up construction frequency domain, normalization, founding mathematical models and calculating are free blood red
The step of protein content, is same as Example 1, and the embodiment of the present invention is not repeated this.
To the model of each device in addition to specified otherwise is done, the model of other devices is not limited the embodiment of the present invention,
As long as the device of above-mentioned function can be completed.
In summary, the embodiments of the invention provide free hemoglobin in a kind of multiposition modulated light source measurement blood bag to contain
The method of amount, solve the problems, such as the Non-Destructive Testing of free hemoglobin in blood bag, it is efficient, easy, pollution-free；Eliminate the spectrum back of the body
The influence of scape noise；The transmitted spectrum that multiposition measuring obtains adds the information content of all the components in blood, greatly suppresses
The influence that blood scattered band comes, improve the analysis precision of free hemoglobin concentration in blood bag.
Embodiment 3
During specific implementation, due to the limitation of space structure, it is possible that light source 1 can not be close to spectrum acquisition device 5
The situation of blood bag 3, at this moment can be by setting an optical fiber, as incident optical 2 respectively at light source 1 and spectrum acquisition device 5
With the output optical fiber 6.
Referring to Fig. 4, the modulated light source 1 of modulating device 7 makes its sender's wave optical signal, and light source 1 is by incident optical 2 to blood bag
Blood sample in 3 is transmitted, and passes through the acquisition of transmission spectrum of the output optical fiber 6 by spectrum acquisition device 5, incident optical 2 is with going out
Penetrate optical fiber 6 and be close to blood bag 3 respectively, a positions are the first position of incident optical 2, and spectrum acquisition device 5 gathers saturating under the position
Penetrate spectrum；Then incident optical 2 is controlled to be moved at the b of the second place by displacement platform 4, spectrum acquisition device 5 gathers the position
Transmitted spectrum under putting；Incident optical 2 is controlled to be moved to always at the n of position by displacement platform 4, spectrum acquisition device 5 gathers
Transmitted spectrum under the position.
Wherein, the transmitted spectrum in follow-up construction frequency domain, normalization, founding mathematical models and calculating are free blood red
The step of protein content, is same as Example 1, and the embodiment of the present invention is not repeated this.
To the model of each device in addition to specified otherwise is done, the model of other devices is not limited the embodiment of the present invention,
As long as the device of above-mentioned function can be completed.
In summary, the embodiments of the invention provide free hemoglobin in a kind of multiposition modulated light source measurement blood bag to contain
The method of amount, solve the problems, such as the Non-Destructive Testing of free hemoglobin in blood bag, it is efficient, easy, pollution-free；Eliminate the spectrum back of the body
The influence of scape noise；The transmitted spectrum that multiposition measuring obtains adds the information content of all the components in blood, greatly suppresses
The influence that blood scattered band comes, improve the analysis precision of free hemoglobin concentration in blood bag.
Embodiment 4
The embodiment of the present invention is different only in that with embodiment 4, the output optical fiber 6, with first position a, second place b ... the
The setting of n positions is different, described below：
Referring to Fig. 5, the modulated light source 1 of modulating device 7 makes its sender's wave optical signal, and light source 1 is by incident optical 2 to blood bag
Blood sample in 3 is transmitted, and passes through the acquisition of transmission spectrum of the output optical fiber 6 by spectrum acquisition device 5, incident optical 2 is with going out
Penetrate optical fiber 6 and be close to blood bag 3 respectively, a positions are the first position of the output optical fiber 6, and spectrum acquisition device 5 gathers saturating under the position
Penetrate spectrum；Then the output optical fiber 6 is controlled to be moved at the b of the second place by displacement platform 4, spectrum acquisition device 5 gathers the position
Transmitted spectrum under putting；The output optical fiber 6 is controlled to be moved to always at the n of position by displacement platform 4, spectrum acquisition device 5 gathers
Transmitted spectrum under the position.
Wherein, the transmitted spectrum in follow-up construction frequency domain, normalization, founding mathematical models and calculating are free blood red
The step of protein content, is same as Example 1, and the embodiment of the present invention is not repeated this.
To the model of each device in addition to specified otherwise is done, the model of other devices is not limited the embodiment of the present invention,
As long as the device of above-mentioned function can be completed.
In summary, the embodiments of the invention provide free hemoglobin in a kind of multiposition modulated light source measurement blood bag to contain
The method of amount, solve the problems, such as the Non-Destructive Testing of free hemoglobin in blood bag, it is efficient, easy, pollution-free；Eliminate the spectrum back of the body
The influence of scape noise；The transmitted spectrum that multiposition measuring obtains adds the information content of all the components in blood, greatly suppresses
The influence that blood scattered band comes, improve the analysis precision of free hemoglobin concentration in blood bag.
Embodiment 5
As different from Example 3, the embodiment only includes incident optical 2 to the embodiment of the present invention, described below：
Referring to Fig. 6, the modulated light source 1 of modulating device 7 makes its sender's wave optical signal, and light source 1 is by incident optical 2 to blood bag
Blood sample in 3 is transmitted, and by the acquisition of transmission spectrum of spectrum acquisition device 5, incident optical 2 enters with spectrum acquisition device 5
Penetrate slit and be close to blood bag 3 respectively, a positions are the first position of incident optical 2, and spectrum acquisition device 5 gathers saturating under the position
Penetrate spectrum；Then incident optical 2 is controlled to be moved at the b of the second place by displacement platform 4, spectrum acquisition device 5 gathers the position
Transmitted spectrum under putting；Incident optical 2 is controlled to be moved to always at the n of position by displacement platform 4, spectrum acquisition device 5 gathers
Transmitted spectrum under the position.
Wherein, the transmitted spectrum in follow-up construction frequency domain, normalization, founding mathematical models and calculating are free blood red
The step of protein content, is same as Example 1, and the embodiment of the present invention is not repeated this.
To the model of each device in addition to specified otherwise is done, the model of other devices is not limited the embodiment of the present invention,
As long as the device of above-mentioned function can be completed.
During specific implementation, can also according to the needs in practical application, to first position a, second place b ... positions n with
And mobile mode is set, i.e., can also include a variety of embodiments, the embodiment of the present invention is without limitation.
In summary, the embodiments of the invention provide free hemoglobin in a kind of multiposition modulated light source measurement blood bag to contain
The method of amount, solve the problems, such as the Non-Destructive Testing of free hemoglobin in blood bag, it is efficient, easy, pollution-free；Eliminate the spectrum back of the body
The influence of scape noise；The transmitted spectrum that multiposition measuring obtains adds the information content of all the components in blood, greatly suppresses
The influence that blood scattered band comes, improve the analysis precision of free hemoglobin concentration in blood bag.
Embodiment 6
As different from Example 3, the embodiment only includes the output optical fiber 6 to the embodiment of the present invention, described below：
Referring to Fig. 7, the modulated light source 1 of modulating device 7 makes its sender's wave optical signal, and light source 1 is to the blood sample in blood bag 3
Transmitted, the acquisition of transmission spectrum of the output optical fiber 6,1 light extraction optical port and the output optical fiber 6 of light source are passed through by spectrum acquisition device 5
It is close to blood bag 3 respectively, a positions are the first position of light source 1, and the output optical fiber 6 gathers the transmitted spectrum under the position；Then control
Displacement platform 4 controls light source 1 to be moved at the b of the second place, and spectrum acquisition device 5 gathers the transmitted spectrum under the position；Control
Displacement platform 4 controls light source 1 to be moved to always at the n of position, and spectrum acquisition device 5 gathers the transmitted spectrum under the position.
Wherein, the transmitted spectrum in follow-up construction frequency domain, normalization, founding mathematical models and calculating are free blood red
The step of protein content, is same as Example 1, and the embodiment of the present invention is not repeated this.
During specific implementation, can also according to the needs in practical application, to first position a, second place b ... positions n with
And mobile mode is set, i.e., can also include a variety of embodiments.
To the model of each device in addition to specified otherwise is done, the model of other devices is not limited the embodiment of the present invention,
As long as the device of above-mentioned function can be completed.
In summary, the embodiments of the invention provide free hemoglobin in a kind of multiposition modulated light source measurement blood bag to contain
The method of amount, solve the problems, such as the Non-Destructive Testing of free hemoglobin in blood bag, it is efficient, easy, pollution-free；Eliminate the spectrum back of the body
The influence of scape noise；The transmitted spectrum that multiposition measuring obtains adds the information content of all the components in blood, greatly suppresses
The influence that blood scattered band comes, improve the analysis precision of free hemoglobin concentration in blood bag.
Embodiment 7
Select, the scheme in above-described embodiment 1-6 is further introduced, light source 1 with reference to specific device
Can be super continuous wide range laser, the super continuous wide range laser covering visible light wave band or near infrared light wave band, or both group
Close, can directly send transmitted light or be conducted through incident optical 2.Displacement platform 4 is stepper motor, and spectrum acquisition device 5 is light
Spectrometer, modulating device 7 is chopper, described below：
As shown in figure 4, chopper 7, which modulates super continuous wide range laser 1, makes its sender's wave optical signal, super continuous wide range laser
1 is transmitted by incident optical 2 to the blood sample in blood bag 3, passes through the acquisition of transmission spectrum of the output optical fiber 6 by spectrometer 5,
Incident optical 2 is close to blood bag 3 respectively with the output optical fiber 6, and a positions are the first position of incident optical 2, and spectrometer 5 gathers the position
Transmitted spectrum under putting；Then incident optical 2 is controlled to be moved at the b of the second place by stepper motor 4, the collection of spectrometer 5 should
Transmitted spectrum under position；Incident optical 2 is controlled to be moved to always at the n of position by stepper motor 4, spectrometer 5 gathers the position
Transmitted spectrum under putting.
Wherein, the transmitted spectrum in follow-up construction frequency domain, normalization, founding mathematical models and calculating are free blood red
The step of protein content, is same as Example 1, and the embodiment of the present invention is not repeated this.
To the model of each device in addition to specified otherwise is done, the model of other devices is not limited the embodiment of the present invention,
As long as the device of above-mentioned function can be completed.
In summary, the embodiments of the invention provide free hemoglobin in a kind of multiposition modulated light source measurement blood bag to contain
The method of amount, solve the problems, such as the Non-Destructive Testing of free hemoglobin in blood bag, it is efficient, easy, pollution-free；Eliminate the spectrum back of the body
The influence of scape noise；The transmitted spectrum that multiposition measuring obtains adds the information content of all the components in blood, greatly suppresses
The influence that blood scattered band comes, improve the analysis precision of free hemoglobin concentration in blood bag.
Embodiment 8
As different from Example 7, light source 1 is bromine tungsten filament lamp wideband light source to the embodiment of the present invention, the bromine tungsten filament lamp wideband light source 1
The combination of covering visible light wave band or near infrared light wave band or both, can directly send transmitted light or be conducted through incident optical 2.
As shown in figure 4, chopper 7, which modulates bromine tungsten filament lamp wideband light source 1, makes its sender's wave optical signal, bromine tungsten filament lamp wideband light source
1 is transmitted by incident optical 2 to the blood sample in blood bag 3, passes through the acquisition of transmission spectrum of the output optical fiber 6 by spectrometer 5,
Incident optical 2 is close to blood bag 3 respectively with the output optical fiber 6, and a positions are the first position of incident optical 2, and spectrometer 5 gathers the position
Transmitted spectrum under putting；Then incident optical 2 is controlled to be moved at the b of the second place by stepper motor 4, the collection of spectrometer 5 should
Transmitted spectrum under position；Incident optical 2 is controlled to be moved to always at the n of position by stepper motor 4, spectrometer 5 gathers the position
Transmitted spectrum under putting.
Wherein, the transmitted spectrum in follow-up construction frequency domain, normalization, founding mathematical models and calculating are free blood red
The step of protein content, is same as Example 1, and the embodiment of the present invention is not repeated this.
To the model of each device in addition to specified otherwise is done, the model of other devices is not limited the embodiment of the present invention,
As long as the device of above-mentioned function can be completed.
In summary, the embodiments of the invention provide free hemoglobin in a kind of multiposition modulated light source measurement blood bag to contain
The method of amount, solve the problems, such as the Non-Destructive Testing of free hemoglobin in blood bag, it is efficient, easy, pollution-free；Eliminate the spectrum back of the body
The influence of scape noise；The transmitted spectrum that multiposition measuring obtains adds the information content of all the components in blood, greatly suppresses
The influence that blood scattered band comes, improve the analysis precision of free hemoglobin concentration in blood bag.
Embodiment 9
For the embodiment of the present invention unlike embodiment 7,8, light source 1 is xenon lamp wide spectrum light source, and the xenon lamp wide spectrum light source covers
Lid visible light wave range or near infrared light wave band, or both combination, can directly send transmitted light or be conducted through incident optical,
It is described below：
As shown in figure 4, chopper 7, which modulates xenon lamp wide spectrum light source 1, makes its sender's wave optical signal, xenon lamp wide spectrum light source 1 leads to
Cross incident optical 2 to transmit the blood sample in blood bag 3, the acquisition of transmission spectrum of the output optical fiber 6 is passed through by spectrometer 5, entered
Penetrate optical fiber 2 and be close to blood bag 3 respectively with the output optical fiber 6, a positions are the first position of incident optical 2, and spectrometer 5 gathers the position
Under transmitted spectrum；Then incident optical 2 is controlled to be moved at the b of the second place by stepper motor 4, spectrometer 5 gathers the position
Transmitted spectrum under putting；Incident optical 2 is controlled to be moved to always at the n of position by stepper motor 4, spectrometer 5 gathers the position
Under transmitted spectrum.
Wherein, the transmitted spectrum in follow-up construction frequency domain, normalization, founding mathematical models and calculating are free blood red
The step of protein content, is same as Example 1, and the embodiment of the present invention is not repeated this.
To the model of each device in addition to specified otherwise is done, the model of other devices is not limited the embodiment of the present invention,
As long as the device of above-mentioned function can be completed.
In summary, the embodiments of the invention provide free hemoglobin in a kind of multiposition modulated light source measurement blood bag to contain
The method of amount, solve the problems, such as the Non-Destructive Testing of free hemoglobin in blood bag, it is efficient, easy, pollution-free；Eliminate the spectrum back of the body
The influence of scape noise；The transmitted spectrum that multiposition measuring obtains adds the information content of all the components in blood, greatly suppresses
The influence that blood scattered band comes, improve the analysis precision of free hemoglobin concentration in blood bag.
Embodiment 10
For the embodiment of the present invention unlike above-described embodiment 7,8,9, needs of the light source 1 in practical application can be with
Other mobile devices can also be used using the light source of other models, displacement platform 4, spectrum acquisition device 5 can also use
Other reception devices.During specific implementation, the embodiment of the present invention is not limited to the model of above-mentioned device.
The embodiment of the present invention is not restricted to position a, position b ... positions n and move mode etc., as long as this hair can be realized
The function of bright embodiment, within the protection domain of the application.
In summary, the embodiments of the invention provide free hemoglobin in a kind of multiposition modulated light source measurement blood bag to contain
The method of amount, solve the problems, such as the Non-Destructive Testing of free hemoglobin in blood bag, it is efficient, easy, pollution-free；Eliminate the spectrum back of the body
The influence of scape noise；The transmitted spectrum that multiposition measuring obtains adds the information content of all the components in blood, greatly suppresses
The influence that blood scattered band comes, improve the analysis precision of free hemoglobin concentration in blood bag.
Claims (10)
1. a kind of method of free hemoglobin content in multiposition modulated light source measurement blood bag, it is characterised in that methods described
Comprise the following steps：
The light extraction optical port of light source is close to blood bag with the entrance slit of spectrum acquisition device, and modulating device modulated light source makes its sender
Wave optical signal, light source transmit to blood sample, spectrum acquisition device acquisition of transmission spectrum；
Displacement platform control light source is moved to multiple positions, by spectrum acquisition device acquisition of transmission spectrum；
The transmitted spectrum that multiple opening positions collect is transformed into frequency domain, constructs transmitted spectrum and normalized in frequency domain,
With the Comparative result of existing chemical analysis, founding mathematical models；
Transmitted spectrum of the unknown blood sample in multiple opening positions is gathered, constructs in frequency domain and brings mathematics into after transmitted spectrum normalization
Model, obtain the content of free hemoglobin；
Methods described changes position by command displacement platform, and the saturating of blood in blood bag is gathered under same light source at the diverse location
Penetrate spectrum；Solves the Non-Destructive Testing of free hemoglobin in blood bag；Multiple station acquisitions to transmitted spectrum increase blood in institute
There is the information content of composition, suppress the influence that blood scattered band comes, eliminate spectral background noise, improve free hemoglobin in blood bag
The analysis precision of concentration.
2. the method for free hemoglobin content in a kind of multiposition modulated light source measurement blood bag according to claim 1,
Characterized in that, it is described construction frequency domain in transmitted spectrum the step of be specially：
Modulating device by modulation of source into square wave optical signal, by spectrum acquisition device acquisition of transmission spectrum, by each wavelength when
Between sequence transformation to frequency domain, with the transmitted spectrum in the fundametal compoment construction frequency domain of each wavelength.
3. the method for free hemoglobin content in a kind of multiposition modulated light source measurement blood bag according to claim 1,
Characterized in that, displacement platform control light source is moved to diverse location, had by the step of spectrum acquisition device acquisition of transmission spectrum
Body is：
Light source transmits at a of position to blood sample, by spectrum acquisition device acquisition of transmission spectrum；
Displacement platform control light source is moved to position b, by spectrum acquisition device acquisition of transmission spectrum；
Displacement platform control light source is moved to position n always, by spectrum acquisition device acquisition of transmission spectrum；
Or,
Light source transmits to the blood sample in blood bag, by spectrum acquisition device in position a acquisition of transmission spectrum；
Displacement platform control spectrum acquisition device is moved to position b, gathers the transmitted spectrum at the b of position；
Displacement platform control spectrum acquisition device is moved to position n, gathers the transmitted spectrum at the n of position.
4. the side of free hemoglobin content in a kind of multiposition modulated light source measurement blood bag according to claim 1 or 2
Method, it is characterised in that methods described also includes：
One optical fiber is set at light source, as incident optical, and ensures that the entrance slit of incident optical and spectrum acquisition device is tight
Paste blood bag；
Or,
One optical fiber is set at spectrum acquisition device, as the output optical fiber, and ensures that the output optical fiber is close to light source light extraction optical port
Blood bag；
Or,
Incident optical and the output optical fiber are set respectively at light source and spectrum acquisition device, and ensure incident optical and the output optical fiber
It is close to blood bag.
5. the method for free hemoglobin content in a kind of multiposition modulated light source measurement blood bag according to claim 3,
Characterized in that,
A positions are the first position of incident optical, and spectrum acquisition device gathers the transmitted spectrum under the position；Control incident optical
It is moved at the b of position, spectrum acquisition device gathers the transmitted spectrum under the position；Control incident optical is moved to position n always
Place, spectrum acquisition device gather the transmitted spectrum under the position.
6. the method for free hemoglobin content in a kind of multiposition modulated light source measurement blood bag according to claim 3,
Characterized in that,
A positions are the first position of the output optical fiber, and spectrum acquisition device gathers the transmitted spectrum under the position；Control the output optical fiber
It is moved at the b of position, spectrum acquisition device gathers the transmitted spectrum under the position；Control the output optical fiber is moved to position n always
Place, spectrum acquisition device gather the transmitted spectrum under the position.
7. the method for free hemoglobin content in a kind of multiposition modulated light source measurement blood bag according to claim 3,
Characterized in that, the light source is super continuous wide range laser, super continuous wide range laser covering visible light wave band, the near-infrared light waves
Section, or both combination, can directly send transmitted light or be conducted through incident optical.
8. the method for free hemoglobin content in a kind of multiposition modulated light source measurement blood bag according to claim 3,
Characterized in that, institute's translation stage is stepper motor；The spectrum acquisition device is spectrometer；The modulating device is copped wave
Device.
9. the method for free hemoglobin content in a kind of multiposition modulated light source measurement blood bag according to claim 3,
Characterized in that, the light source is xenon lamp wide spectrum light source or bromine tungsten filament lamp wide spectrum light source, the xenon lamp wide spectrum light source or bromine tungsten filament lamp wide range
Light source covering visible light wave band or near infrared light wave band, or both combination, can directly send transmitted light or be passed through incident optical
Lead.
10. dissociate blood in a kind of multiposition modulated light source measurement blood bag according to any claim in claim 1-3
The method of Lactoferrin content, it is characterised in that
The mathematical modeling utilizes principal component analysis, artificial neural network, PLS, SVMs, signal point
Analysis or statistical method are established.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710529561.4A CN107356533A (en) | 2017-07-02 | 2017-07-02 | The method of free hemoglobin content in multiposition modulated light source measurement blood bag |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710529561.4A CN107356533A (en) | 2017-07-02 | 2017-07-02 | The method of free hemoglobin content in multiposition modulated light source measurement blood bag |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107356533A true CN107356533A (en) | 2017-11-17 |
Family
ID=60273780
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710529561.4A Pending CN107356533A (en) | 2017-07-02 | 2017-07-02 | The method of free hemoglobin content in multiposition modulated light source measurement blood bag |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107356533A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111505263A (en) * | 2019-01-31 | 2020-08-07 | 青岛海尔生物医疗股份有限公司 | Blood quality detection method and blood quality detection device |
| CN111505262A (en) * | 2019-01-31 | 2020-08-07 | 青岛海尔生物医疗股份有限公司 | Blood quality detection method and blood quality detection device |
| EP4123303A1 (en) | 2021-07-20 | 2023-01-25 | German Red Cross Blood Donor Service Baden-Württemberg-Hessen gGmbH | Non-invasive method for predicting the hemoglobin and iron content in prbc units |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060167347A1 (en) * | 2002-11-04 | 2006-07-27 | Kexin Xu | Composite spectral measurement method and its spectral detection instrument |
| CN101793678A (en) * | 2010-02-22 | 2010-08-04 | 天津大学 | Spectrum measuring device and method of scattering substance of sample cell with isosceles triangle cross section |
| CN101813623A (en) * | 2010-04-22 | 2010-08-25 | 天津大学 | Scatterer multi-dimensional spectral measuring unit and method |
| CN102033048A (en) * | 2010-12-31 | 2011-04-27 | 天津大学 | Measuring method of absorption spectrum for eliminating ambient stray light interference |
| CN105593684A (en) * | 2013-10-01 | 2016-05-18 | 豪夫迈·罗氏有限公司 | Method for recognising state of sample, device for analysing samples and laboratory automation system |
-
2017
- 2017-07-02 CN CN201710529561.4A patent/CN107356533A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060167347A1 (en) * | 2002-11-04 | 2006-07-27 | Kexin Xu | Composite spectral measurement method and its spectral detection instrument |
| CN101793678A (en) * | 2010-02-22 | 2010-08-04 | 天津大学 | Spectrum measuring device and method of scattering substance of sample cell with isosceles triangle cross section |
| CN101813623A (en) * | 2010-04-22 | 2010-08-25 | 天津大学 | Scatterer multi-dimensional spectral measuring unit and method |
| CN102033048A (en) * | 2010-12-31 | 2011-04-27 | 天津大学 | Measuring method of absorption spectrum for eliminating ambient stray light interference |
| CN105593684A (en) * | 2013-10-01 | 2016-05-18 | 豪夫迈·罗氏有限公司 | Method for recognising state of sample, device for analysing samples and laboratory automation system |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111505263A (en) * | 2019-01-31 | 2020-08-07 | 青岛海尔生物医疗股份有限公司 | Blood quality detection method and blood quality detection device |
| CN111505262A (en) * | 2019-01-31 | 2020-08-07 | 青岛海尔生物医疗股份有限公司 | Blood quality detection method and blood quality detection device |
| CN111505263B (en) * | 2019-01-31 | 2023-11-17 | 青岛海特生物医疗有限公司 | Blood quality detection method and blood quality detection device |
| CN111505262B (en) * | 2019-01-31 | 2023-11-17 | 青岛海特生物医疗有限公司 | Blood quality detection method and blood quality detection device |
| EP4123303A1 (en) | 2021-07-20 | 2023-01-25 | German Red Cross Blood Donor Service Baden-Württemberg-Hessen gGmbH | Non-invasive method for predicting the hemoglobin and iron content in prbc units |
| WO2023001889A1 (en) | 2021-07-20 | 2023-01-26 | Drk Blutspendedienst Baden-Württemberg-Hessen Ggmbh | Non-invasive method for predicting the hemoglobin and iron content in prbc units |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104897607B (en) | Portable near infrared spectrum food modeling and quick detection integral method and system | |
| CN107356533A (en) | The method of free hemoglobin content in multiposition modulated light source measurement blood bag | |
| CN107367484A (en) | A kind of method of free hemoglobin content in nondestructive measurement blood bag | |
| CN105628671B (en) | A kind of device and method for sample component quantitative analysis | |
| CN107044959A (en) | Micro- multi-modal fusion spectral detection system | |
| CN105527246A (en) | Portable near infrared spectrum detection system | |
| CN105380609A (en) | Multi-spectrum based skin detection method and system | |
| CN106841065A (en) | Ultraviolet-visible near-infrared transflector spectral measurement device and measuring method | |
| CN107421901A (en) | The method of free hemoglobin content in multiposition transmitted spectrum measurement blood bag | |
| CN107655832A (en) | Based on the femtosecond Two-dimensional spectroscopy acquisition method changed in chirp and system | |
| CN107389560A (en) | Multiband all -fiber high spectral resolution total atmospheric spectral transmittance simultaneous measuring apparatus and measuring method | |
| CN205215187U (en) | Skin detecting system based on it is multispectral | |
| CN105334166A (en) | Dual-detector near-infrared spectroscopy used for food composition analysis | |
| CN107167441A (en) | The method that double light path and multiposition modulated light sources measure complicated solution component content | |
| CN107167440A (en) | The method of free hemoglobin content in double optical path modulation light source measurement blood bags | |
| CN107167432A (en) | The method that multiposition modulated light source measures packed complicated solution component content | |
| CN208076389U (en) | A kind of terahertz light spectrometer | |
| CN107179284A (en) | The method of double packed complicated solution component contents of optical path modulation light source measurement | |
| CN107179283A (en) | A kind of method of the packed complicated solution component content of nondestructive measurement | |
| CN107421922A (en) | Double light path transmissions and the method for fluorescence spectral measuring free hemoglobin content | |
| CN107421903A (en) | The method of double light path and multiposition modulated light source measurement free hemoglobin contents | |
| CN107356575A (en) | The method that multiposition modulation fluorescence excitation light source measures packed complicated solution composition | |
| CN107421925A (en) | Multiposition modulation transmission and the method for fluorescence excitation light source measurement free hemoglobin | |
| CN107167477A (en) | Lower pair of light path transmission of multiposition and the method for fluorescence spectral measuring complicated solution composition | |
| CN107421902A (en) | The method of double light path and multiposition transmitted spectrum measurement free hemoglobin contents |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20171117 |
|
| RJ01 | Rejection of invention patent application after publication |