CN107167440A - The method of free hemoglobin content in double optical path modulation light source measurement blood bags - Google Patents
The method of free hemoglobin content in double optical path modulation light source measurement blood bags Download PDFInfo
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- G01N21/314—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry with comparison of measurements at specific and non-specific wavelengths
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Abstract
The invention discloses the method for free hemoglobin content in a kind of pair of optical path modulation light source measurement blood bag, including：Modulating device is modulated to light source, and light source light extraction optical port is close to blood bag and coaxial with spectrum acquisition device entrance slit, and light source is transmitted to blood sample, spectrum acquisition device acquisition of transmission spectrum；Displacement platform control light source movement under the premise of ensureing that light source light extraction optical port and spectrum acquisition device entrance slit are coaxial, by spectrum acquisition device acquisition of transmission spectrum；The time series of each wavelength of transmitted spectrum is transformed into frequency domain, with the transmitted spectrum in the fundametal compoment construction frequency domain of each wavelength, logarithm is asked to be absorbed spectrum light intensity ratio under each wavelength of transmitted spectrum in two frequency domains of construction, with the Comparative result of existing chemical analysis, founding mathematical models after normalized；Same method gathers the absorption spectrum of unknown blood, brings mathematical modeling into after being normalized, obtains the content of free hemoglobin.
Description
Technical field
The present invention relates to spectrum complicated solution concentration analysis stoichiometry field, more particularly to a kind of pair of optical path modulation light source
The method for measuring free hemoglobin content in blood bag.
Background technology
In the prior art, more ripe technology is that containing for free hemoglobin in blood bag is detected by chemical analysis
Amount, the mode with the high outstanding advantages of accuracy, but chemical analysis can not meet quickly, noncontact and free of contamination need
Ask, spectral measurement is also possible to realize the content detection of free hemoglobin in blood bag due to its noncontact, free of contamination characteristic.
In spectral detection, according to Lambert-Beer's law：The incident intensity I of each wavelength is measured respectively0And output intensity
I, the absorbance A of each wavelength is calculated by formula (1).∈ is material absorptivity under a certain wavelength, and c is dense for material
Degree, b is optical path length.
In fact, failing measurement incident intensity I for various reasons0, for example：Incident intensity I0It is difficult to measure by force very much, but
If in incident intensity I0It is basicly stable it is constant in the case of, only measurement an output intensity I can also obtain good result.But
Spectral detection is influenceed by spectral background noise, the influence of light source change and the influence of measurement container are difficult to reach that measurement is needed
The precision wanted.
The influence of light source is mainly shown as the change of spatial distribution and light intensity.The reason for light source changes is caused to have a lot, such as
Light source voltage change, filament aging or variation of ambient temperature etc..In spectrum analysis, rarely have document to introduce light source to measurement essence
The influence of degree, and reduce the method that intensity of light source change influences on measurement accuracy.In research in the early time, with the mode of calibration
To eliminate some interference, such as calibrated with water, but be due to that light intensity is too strong, be difficult to operate in practice.Also many scholars utilize
Neutral filter or optical fibre light splitting mode measure incident intensity I0.(discussion below is said except non-specifically by taking neutral filter as an example
It is bright, discussed on some wavelength), the output intensity I that measurement passes through neutral filtern, then the light intensity I of light source0 nSuction can be used
Luminosity A and output intensity InTo represent：
Then sample is replaced into neutral filter, measures the output intensity I of sampleS：
NoticeSo
Do not have in the final result of formula (4)(namely) occur, illustrate that the intensity (and its spectrum) of light source is not interfered with
Measurement to sample, as long as all measurements keep lgI all using the calibration of same neutral attenuatorn+AnFor constant constant.
Haveed the shortcomings that using the above method as follows：
Different occasions are difficult to find duplicate neutral filter, and it is difficult to ensure that the position of sample and neutral filter
Unanimously.
The content of the invention
The invention provides the method for the free hemoglobin content of blood in a kind of pair of light path transmitted spectrum measurement blood bag,
It is workable, the influence that spectral background noise, light source change and blood bag is brought is eliminated, free hemoglobin content is improved
Analysis precision, it is described below：
The method of free hemoglobin content in a kind of pair of optical path modulation light source measurement blood bag, methods described includes following step
Suddenly：
Light source light extraction optical port is close to blood bag with spectrum acquisition device entrance slit, and modulating device modulated light source makes its sender
Wave optical signal, light source is transmitted to blood sample, spectrum acquisition device acquisition of transmission spectrum；
Displacement platform is under the premise of ensureing that light source light extraction optical port and spectrum acquisition device entrance slit are coaxial, and control light source is moved
It is dynamic, by spectrum acquisition device acquisition of transmission spectrum；
Transmitted spectrum at two positions is transformed into transmitted spectrum in frequency domain construction frequency domain respectively, will be transmitted in two frequency domains
Light intensity ratio asks logarithm to be absorbed spectrum under each wavelength of spectrum, will after absorption spectrum normalized with existing chemistry point
The Comparative result of analysis, founding mathematical models；
The transmitted spectrum at unknown blood two positions is gathered, two transmitted spectrums are transformed in frequency domain construction frequency domain thoroughly respectively
Spectrum is penetrated, asks logarithm to be absorbed spectrum light intensity ratio under each wavelength of transmitted spectrum in two frequency domains, by absorption spectrum
It is normalized, and brings mathematical modeling calculating into, obtains the content of free hemoglobin；
Methods described changes light path by command displacement platform, and the blood under same modulated light source is gathered in different light path strong points
The transmitted spectrum of blood in bag, eliminates the influence that light source change and blood bag are brought, eliminates the influence of spectral background noise, improve trip
From the analysis precision of content of hemoglobin, the Non-Destructive Testing of free hemoglobin content in blood bag is solved.
Wherein, it is specially the step of transmitted spectrum in the construction frequency domain：
Modulating device by modulation of source into square wave optical signal, by spectrum acquisition device acquisition of transmission spectrum, by transmitted spectrum
The time series of each wavelength transform to frequency domain, with transmitted spectrum in the fundametal compoment construction frequency domain of each wavelength.
Wherein, control light source movement, by spectrum acquisition device acquisition of transmission spectrum the step of be specially：
Light source is transmitted at a of position to blood sample, by spectrum acquisition device acquisition of transmission spectrum；
Displacement platform control light source is moved to position b, by spectrum acquisition device acquisition of transmission spectrum；
Or,
Light source is transmitted to the blood sample in blood bag, by spectrum acquisition device in position a acquisition of transmission spectrum；
The transmitted spectrum that displacement platform control spectrum acquisition device is moved at position b, collection position b；
Or,
The blood sample in blood bag is transmitted by light source at a of position, adopted at position a ' places by spectrum acquisition device
Collect transmitted spectrum；
Displacement platform controls light source and spectrum acquisition device to be respectively moved to position b, b ' place, gathered by spectrum acquisition device
Transmitted spectrum.
Wherein, methods described also includes：
One optical fiber is set at light source, as incident optical, and ensures incident optical and spectrum acquisition device entrance slit
It is close to blood bag and coaxial；
Or,
One optical fiber is set at spectrum acquisition device, as the output optical fiber, and ensures the output optical fiber and light source light extraction optical port
It is close to blood bag and coaxial；
Or,
Incident optical and the output optical fiber are set respectively at light source and spectrum acquisition device, and ensure incident optical and outgoing
Optical fiber is close to blood bag and coaxial.
Wherein, a positions are the first position of incident optical, and incident optical and emergent light are gathered by spectrum acquisition device
Transmitted spectrum under fine relative position a；On the premise of guarantee incident optical and the output optical fiber are coaxial, control incident optical movement
To at the b of position, incident optical and the transmitted spectrum under the output optical fiber relative position b are gathered by spectrum acquisition device.
Wherein, a positions are the first position of the output optical fiber, and incident optical and emergent light are gathered by spectrum acquisition device
Transmitted spectrum under fine relative position a；On the premise of guarantee incident optical and the output optical fiber are coaxial, control the output optical fiber movement
To at the b of position, incident optical and the transmitted spectrum under the output optical fiber relative position b are gathered by spectrum acquisition device.
Wherein, a, a ' be respectively incident optical and the output optical fiber first position, by spectrum acquisition device gather incident light
The fine position a, a relative with the output optical fiber ' under transmitted spectrum；On the premise of guarantee incident optical and the output optical fiber are coaxial,
Control incident optical and the output optical fiber are moved to position b, b ' place, incident optical and the output optical fiber phase are gathered by spectrum acquisition device
To position b, b ' under transmitted spectrum.
Further, the light source is super continuous wide range laser, xenon lamp wide spectrum light source or bromine tungsten filament lamp wideband light source, the super company
Continuous wide range laser, xenon lamp wide spectrum light source or bromine tungsten filament lamp wideband light source covering visible light wave band or near infrared light wave band, or both
Combination, can directly light or be conducted through incident optical.
Further, institute's translation stage is stepper motor or magnet attraction device；The spectrum acquisition device is spectrum
Instrument；The modulating device is chopper.
Mathematical modeling described above utilizes principal component analysis, artificial neural network, PLS, supporting vector
Machine, signal analysis or statistical method are set up.
The beneficial effect for the technical scheme that the present invention is provided is：The present invention changes light path by command displacement platform, not
The transmitted spectrum of blood in the blood bag under same modulated light source is gathered with light path strong point, light source change and blood bag band is not only eliminated
The influence come, and the influence of spectral background noise is eliminated, the analysis precision of free hemoglobin content is improved, is solved
The Non-Destructive Testing problem of free hemoglobin content, efficient, easy, pollution-free in blood bag, workable.
Brief description of the drawings
Fig. 1 is the method schematic of free hemoglobin content in double optical path modulation light source measurement blood bags；
Fig. 2 is double light path transmission spectrum method principle schematics；
Fig. 3 is the method schematic diagram of free hemoglobin content in double optical path modulation light source measurement blood bags in embodiment 1；
Fig. 4 is another signal of method of free hemoglobin content in double optical path modulation light source measurement blood bags in embodiment 2
Figure；
Fig. 5 is another signal of method of free hemoglobin content in double optical path modulation light source measurement blood bags in embodiment 3
Figure；
Fig. 6 is another signal of method of free hemoglobin content in double optical path modulation light source measurement blood bags in embodiment 4
Figure；
Fig. 7 is another signal of method of free hemoglobin content in double optical path modulation light source measurement blood bags in embodiment 5
Figure；
Fig. 8 is another signal of method of free hemoglobin content in double optical path modulation light source measurement blood bags in embodiment 6
Figure；
Fig. 9 is another signal of method of free hemoglobin content in double optical path modulation light source measurement blood bags in embodiment 7
Figure；
Figure 10 is another signal of method of free hemoglobin content in double optical path modulation light source measurement blood bags in embodiment 8
Figure.
In accompanying drawing, the list of parts representated by each label is as follows：
1：First light path； 2：Second light path；
3：Light source； 4：Incident optical；
5：Blood bag； 6：Displacement platform；
7：Spectrum acquisition device； 8：The output optical fiber；
9：Modulating device； a、a’：First position；
b、b’：The second place.
Embodiment
To make the object, technical solutions and advantages of the present invention clearer, further is made to embodiment of the present invention below
It is described in detail on ground.
When modulating device is positioned among light source optical path, light can periodically by with block.Now light source is sent
Light is modulated into the square wave optical signal with certain frequency.The frequency and light of each wavelength in the transmitted spectrum that transmission blood is obtained
The square wave light frequency that source is sent is consistent, by taking a certain wavelength X of transmitted spectrum as an example, and Fig. 1 is the waveform and spectrum acquisition device of λ wavelength
The time of integration corresponding integrated value schematic diagram, B is ambient noise, and T is the time of integration of spectrum acquisition device, and T1=T2=...
=Ti=T；T1It is ambient noise that the time of integration is corresponding in interval, the light intensity amplitude background that now spectrum acquisition device is received
The integrated value of noise, numerical value minimum is designated as Imin；TiIn interval the time of integration it is corresponding be λ wavelength light intensity and ambient noise, this
The light intensity amplitude that Shi Guangpu reception devices are received is the light intensity of λ wavelength and the integrated value of ambient noise, and numerical value maximum is designated as
Imax, T1With TiBetween other times of integration part correspondence ambient noises, the light intensity and background of another part correspondence λ wavelength are made an uproar
Sound, therefore resulting integrated value is in (Imin, Imax) interval interior variation.Thus, in T1~TiThe interior class value domain that can obtain is
(Imin, Imax) integration value sequence, it can be seen that, the integrated value of the wavelength is in (Imin, Imax) it is interval in change and formed periodically
Signal, the integrated value of other wavelength is similar, and for strictly with cycle and synchronous cyclical signal.By to each wavelength
The time series of integrated value carry out Fourier transform, it is saturating in the frequency domain constituted with the frequency domain fundametal compoment of all wavelengths integrated value
Spectrum is penetrated, spectral background noise can be eliminated, signal to noise ratio is increased substantially.
Double light path methods are according to Lambert-Beer's law, as shown in Fig. 2 setting the first light path 1 and the second light path 2 respectively.Push away
Lead process as follows：
Wherein, A1It is the absorbance of the first light path 1, A2It is the absorbance of the second light path 2.IOIt is the incident light of the first light path 1
Light intensity, while being also the light intensity of the incident light of the second light path 2, I1It is the output intensity of the first light path 1, I2It is the second light path 2
Output intensity, b1It is the optical length of the first light path 1, b2It is the optical length of the second light path 2, △ b are the difference of two optical lengths, ∈ extinctions
Coefficient is that c surveys material concentration.
The absorbance of double light path spectroscopic methodologies is still linear with optical path difference it can be seen from formula (7), meet lambert-
Beer law, and with light intensity of incident light IOIt is unrelated.Therefore, double light path methods are not influenceed by light source in theory, are deducted simultaneously
The influence of blood bag in itself.
Embodiment 1
The method of free hemoglobin content, is made in provided in an embodiment of the present invention pair of optical path modulation light source measurement blood bag
The device used as shown in figure 3, including：Light source 3, blood bag 5, displacement platform 6, spectrum acquisition device 7 and modulating device 9.
Wherein, it is ensured that the light extraction optical port of light source 3 is close to blood bag 5 and coaxial, modulating device with the entrance slit of spectrum acquisition device 7
9 modulated light sources 3 make its sender's wave optical signal, and light source 3 is at first position a (the first light path 1 of correspondence) place to the blood in blood bag 5
Sample is transmitted, by the acquisition of transmission spectrum of spectrum acquisition device 7.The light extraction optical port of light source 3 is then being ensured by displacement platform 6
With the exit slit of spectrum acquisition device 7 it is coaxial on the premise of control light source to be moved to second place b (correspondence the second light path 2), by
The acquisition of transmission spectrum of spectrum acquisition device 7.
The time series of each wavelength of the transmitted spectrum gathered at two positions of a, b is transformed into frequency domain, with each ripple
Transmitted spectrum in long fundametal compoment construction frequency domain, thus eliminates the influence of spectral background noise, by transmitted light in two frequency domains
Light intensity ratio asks logarithm to be absorbed spectrum under each wavelength of spectrum, thus eliminates the measurement mistake that flashing is fixed and blood bag is brought
Difference, more objectively responds the change of blood constituent between individual.By absorption spectrum normalized, method for normalizing is：
Ag=A/max (A) (8)
In formula (8), AgFor normalization absorbance, max (A) is the absorbance maximum on different wave length, and A is extinction
Degree.With reference to the data of chemical analysis, principal component analysis (PCA, principal component analysis) or artificial is utilized
Neutral net (ANN, artificial neural network) or PLS (PLSR, particle least
Squares calibration analysis) or SVMs (SVM, support vector machines) signal point
The method such as analysis or statistics can founding mathematical models.
The step of embodiment of the present invention is to specific founding mathematical models are not repeated, known to those skilled in the art.
The transmitted spectrum of position at unknown blood sample a, b two is gathered, by the time series of each wavelength of transmitted spectrum
Frequency domain is transformed to, with transmitted spectrum in the fundametal compoment construction frequency domain of each wavelength, transmitted spectrum in two frequency domains is absorbed into light
Spectrum, which is normalized, brings the above-mentioned mathematical modeling established into and is calculated, and obtains the content of free hemoglobin.
The embodiment of the present invention is to the model of each device in addition to specified otherwise is done, and the model of other devices is not limited,
As long as the device of above-mentioned functions can be completed.
In summary, contain the embodiments of the invention provide free hemoglobin in a kind of pair of optical path modulation light source measurement blood bag
The method of amount, it is workable, the influence that spectral background noise, light source change and blood bag is brought is eliminated, free blood is improved
The analysis precision of Lactoferrin content, solves the problems, such as the Non-Destructive Testing of free hemoglobin content in blood bag, efficient, easy, nothing
Pollution.
Embodiment 2
The embodiment of the present invention is differed only in embodiment 1, and the move mode of light source 3 and spectrum acquisition device 7 is not
Together, it is described below：
Referring to Fig. 4, it is ensured that the light extraction optical port of light source 3 is close to blood bag 5 and coaxial with the entrance slit of spectrum acquisition device 7, modulation
The modulated light source 3 of device 9 makes its sender's wave optical signal, and light source 3 is transmitted to the blood sample in blood bag 5, by spectrum acquisition
The acquisition of transmission spectrum at a of first position of device 7.The light extraction optical port of light source 3 and spectrum acquisition are then being ensured by displacement platform 6
On the premise of the entrance slit of device 7 is coaxial, control spectrum acquisition device 7 is moved at second place b, collection second place b
Transmitted spectrum.
Wherein, transmitted spectrum, construction absorption spectrum, normalization, founding mathematical models, Yi Jiji in follow-up construction frequency domain
The step of calculating free hemoglobin content is same as Example 1, and the embodiment of the present invention is not repeated this.
The embodiment of the present invention is to the model of each device in addition to specified otherwise is done, and the model of other devices is not limited,
As long as the device of above-mentioned functions can be completed.
In summary, contain the embodiments of the invention provide free hemoglobin in a kind of pair of optical path modulation light source measurement blood bag
The method of amount, it is workable, the influence that spectral background noise, light source change and blood bag is brought is eliminated, free blood is improved
The analysis precision of Lactoferrin content, solves the problems, such as the Non-Destructive Testing of free hemoglobin content in blood bag, efficient, easy, nothing
Pollution.
Embodiment 3
The embodiment of the present invention is differed only in embodiment 1, and the moving direction of light source 3 and spectrum acquisition device 7 is not
Together, it is described below：
Referring to Fig. 5, it is ensured that light source 3 is close to blood bag 5 with spectrum acquisition device 7 and ensures that the light extraction optical port of light source 3 and spectrum connect
The entrance slit of receiving apparatus 7 is coaxial, and the modulated light source 3 of modulating device 9 makes its sender's wave optical signal, by light source 3 at a of first position
Blood sample in blood bag 5 is transmitted, it is then logical at first position a ' places by the acquisition of transmission spectrum of spectrum acquisition device 7
Displacement platform 6 is crossed on the premise of ensureing that the light extraction optical port of light source 3 and the entrance slit of spectrum acquisition device 7 are coaxial, the He of light source 3 is controlled
Spectrum acquisition device 7 is respectively moved to the second place b, b ' place, by the acquisition of transmission spectrum of spectrum acquisition device 7.
Wherein, transmitted spectrum, construction absorption spectrum, normalization, founding mathematical models, Yi Jiji in follow-up construction frequency domain
The step of calculating free hemoglobin content is same as Example 1, and the embodiment of the present invention is not repeated this.
The embodiment of the present invention is to the model of each device in addition to specified otherwise is done, and the model of other devices is not limited,
As long as the device of above-mentioned functions can be completed.
In summary, contain the embodiments of the invention provide free hemoglobin in a kind of pair of optical path modulation light source measurement blood bag
The method of amount, it is workable, the influence that spectral background noise, light source change and blood bag is brought is eliminated, free blood is improved
The analysis precision of Lactoferrin content, solves the problems, such as the Non-Destructive Testing of free hemoglobin content in blood bag, efficient, easy, nothing
Pollution.
Embodiment 4
When implementing, due to the limitation of space structure, it is possible that light source 3 can not be close to spectrum acquisition device 7
The situation of blood bag 5, at this moment can be used as incident optical 4 by setting an optical fiber respectively at light source 3 and spectrum acquisition device 7
With the output optical fiber 8.
Referring to Fig. 6, the modulated light source 3 of modulating device 9 makes its sender's wave optical signal, and light source 3 is by incident optical 4 to blood bag
Blood sample in 5 is transmitted, and passes through the acquisition of transmission spectrum of the output optical fiber 8 by spectrum acquisition device 7, incident optical 4 is with going out
Penetrate optical fiber 8 to be close to blood bag 3 respectively and ensure coaxial, a positions are the first position of incident optical 4, spectrum acquisition device 7 gather into
Optical fiber 4 is penetrated with the output optical fiber 8 with respect to the transmitted spectrum under a of the first position；Incident optical is then being ensured by displacement platform 6
4 with the position of the output optical fiber 8 remain unchanged it is coaxial on the premise of, control incident optical 4 be moved at the b of the second place, spectrum acquisition device 7
Gather the transmitted spectrum under the incident optical 4 and relative second position b of the output optical fiber 8.
Wherein, transmitted spectrum, construction absorption spectrum, normalization, founding mathematical models, Yi Jiji in follow-up construction frequency domain
The step of calculating free hemoglobin content is same as Example 1, and the embodiment of the present invention is not repeated this.
The embodiment of the present invention is to the model of each device in addition to specified otherwise is done, and the model of other devices is not limited,
As long as the device of above-mentioned functions can be completed.
In summary, contain the embodiments of the invention provide free hemoglobin in a kind of pair of optical path modulation light source measurement blood bag
The method of amount, it is workable, the influence that spectral background noise, light source change and blood bag is brought is eliminated, free blood is improved
The analysis precision of Lactoferrin content, solves the problems, such as the Non-Destructive Testing of free hemoglobin content in blood bag, efficient, easy, nothing
Pollution.
Embodiment 5
The embodiment of the present invention is different only in that with embodiment 4, the output optical fiber 8, sets with first position a, second place b
Difference is put, it is described below：
Referring to Fig. 7, the modulated light source 3 of modulating device 9 makes its sender's wave optical signal, and light source 3 is by incident optical 4 to blood bag
Blood sample in 5 is transmitted, and passes through the acquisition of transmission spectrum of the output optical fiber 8 by spectrum acquisition device 7, incident optical 4 is with going out
Penetrate optical fiber 8 to be close to blood bag 3 respectively and ensure coaxial, a positions are the first position of the output optical fiber 8, spectrum acquisition device 7 gather into
Optical fiber 4 is penetrated with the output optical fiber 8 with respect to the transmitted spectrum under a of the first position；Incident optical is then being ensured by displacement platform 6
4 with the position of the output optical fiber 8 remain unchanged it is coaxial on the premise of, control the output optical fiber 8 be moved at the b of the second place, spectrum acquisition device 7
Gather the transmitted spectrum under the incident optical 4 and relative second position b of the output optical fiber 8.
Wherein, transmitted spectrum, construction absorption spectrum, normalization, founding mathematical models, Yi Jiji in follow-up construction frequency domain
The step of calculating free hemoglobin content is same as Example 1, and the embodiment of the present invention is not repeated this.
The embodiment of the present invention is to the model of each device in addition to specified otherwise is done, and the model of other devices is not limited,
As long as the device of above-mentioned functions can be completed.
In summary, contain the embodiments of the invention provide free hemoglobin in a kind of pair of optical path modulation light source measurement blood bag
The method of amount, it is workable, the influence that spectral background noise, light source change and blood bag is brought is eliminated, free blood is improved
The analysis precision of Lactoferrin content, solves the problems, such as the Non-Destructive Testing of free hemoglobin content in blood bag, efficient, easy, nothing
Pollution.
Embodiment 6
The embodiment of the present invention is different only in that with embodiment 4, incident optical 4, the output optical fiber 8, with first position a, the
Two position b setting is different, described below：
Referring to Fig. 8, the modulated light source 3 of modulating device 9 makes its sender's wave optical signal, and light source 3 is by incident optical 4 to blood bag
Blood sample in 5 is transmitted, and passes through the acquisition of transmission spectrum of the output optical fiber 8 by spectrum acquisition device 7, incident optical 4 is with going out
Optical fiber 8 is penetrated to be close to blood bag 3 respectively and ensure coaxial, a, a ' be respectively incident optical 4 and the output optical fiber 8 first position, spectrum
Reception device 7 gathers transmitted spectrum of the incident optical 4 with the output optical fiber 8 under relative first position a, a '；Then pass through displacement
Platform 6 ensure incident optical 4 and the position of the output optical fiber 8 remain unchanged it is coaxial on the premise of, control incident optical 4 and the output optical fiber 8
It is moved respectively to the second place b, b ' place, the collection of spectrum acquisition device 7 incident optical 4 and the relative second position b of the output optical fiber 8,
Transmitted spectrum under b '.
Wherein, the step of content of follow-up normalization, founding mathematical models and calculating free hemoglobin is with implementing
Example 1 is identical, and the embodiment of the present invention is not repeated this.
The embodiment of the present invention is to the model of each device in addition to specified otherwise is done, and the model of other devices is not limited,
As long as the device of above-mentioned functions can be completed.
In summary, contain the embodiments of the invention provide free hemoglobin in a kind of pair of optical path modulation light source measurement blood bag
The method of amount, it is workable, the influence that spectral background noise, light source change and blood bag is brought is eliminated, free blood is improved
The analysis precision of Lactoferrin content, solves the problems, such as the Non-Destructive Testing of free hemoglobin content in blood bag, efficient, easy, nothing
Pollution.
Embodiment 7
As different from Example 4, the embodiment only includes the embodiment of the present invention：Incident optical 4, it is described below：
Referring to Fig. 9, the modulated light source 3 of modulating device 9 makes its sender's wave optical signal, and light source 3 is by incident optical 4 to blood bag
Blood sample in 5 is transmitted, and by the acquisition of transmission spectrum of spectrum acquisition device 7, incident optical 4 enters with spectrum acquisition device 7
Penetrate slit to be close to blood bag 3 respectively and ensure coaxial, a positions are the first position of incident optical 4, spectrum acquisition device 7 gather into
Optical fiber 4 is penetrated with respect to the transmitted spectrum under a of the first position；Incident optical 4 and spectrum acquisition are then being ensured by displacement platform 6
On the premise of the slit location of device 7 remains unchanged coaxially, control incident optical 4 is moved at the b of the second place, and spectrum acquisition device 7 is adopted
Collect the transmitted spectrum under the relative second position b of incident optical 4.
Wherein, transmitted spectrum, construction absorption spectrum, normalization, founding mathematical models, Yi Jiji in follow-up construction frequency domain
The step of calculating free hemoglobin content is same as Example 1, and the embodiment of the present invention is not repeated this.
The embodiment of the present invention is to the model of each device in addition to specified otherwise is done, and the model of other devices is not limited,
As long as the device of above-mentioned functions can be completed.
, can also be according to the need in practical application, to first position a, second place b and mobile when implementing
Mode set, i.e., can also include a variety of embodiments, the embodiment of the present invention is without limitation.
In summary, contain the embodiments of the invention provide free hemoglobin in a kind of pair of optical path modulation light source measurement blood bag
The method of amount, it is workable, the influence that spectral background noise, light source change and blood bag is brought is eliminated, free blood is improved
The analysis precision of Lactoferrin content, solves the problems, such as the Non-Destructive Testing of free hemoglobin content in blood bag, efficient, easy, nothing
Pollution.
Embodiment 8
As different from Example 4, the embodiment only includes the embodiment of the present invention：The output optical fiber 8, it is described below：
Referring to Figure 10, the modulated light source 3 of modulating device 9 makes its sender's wave optical signal, and light source 3 is to the blood sample in blood bag 5
Product are transmitted, and pass through the acquisition of transmission spectrum of the output optical fiber 8, the light extraction optical port of light source 3 and the output optical fiber 8 by spectrum acquisition device 7
Be close to blood bag 3 respectively and ensure coaxial, spectrum acquisition device 7 gather light source 3 with it is saturating under the relative first position a of the output optical fiber 8
Penetrate spectrum；Then by displacement platform 6 ensure the light extraction optical port of light source 3 and the position of the output optical fiber 8 remain unchanged it is coaxial on the premise of, control
Light source 3 processed is moved at the b of the second place, spectrum acquisition device 7 gather light source 3 with it is saturating under the relative second position b of the output optical fiber 8
Penetrate spectrum.
Wherein, transmitted spectrum, construction absorption spectrum, normalization, founding mathematical models, Yi Jiji in follow-up construction frequency domain
The step of calculating free hemoglobin content is same as Example 1, and the embodiment of the present invention is not repeated this.
, can also be according to the need in practical application, to first position a, second place b and mobile when implementing
Mode set, i.e., can also include a variety of embodiments.
The embodiment of the present invention is to the model of each device in addition to specified otherwise is done, and the model of other devices is not limited,
As long as the device of above-mentioned functions can be completed.
In summary, contain the embodiments of the invention provide free hemoglobin in a kind of pair of optical path modulation light source measurement blood bag
The method of amount, it is workable, the influence that spectral background noise, light source change and blood bag is brought is eliminated, free blood is improved
The analysis precision of Lactoferrin content, solves the problems, such as the Non-Destructive Testing of free hemoglobin content in blood bag, efficient, easy, nothing
Pollution.
Embodiment 9
Select, the scheme in above-described embodiment 1-6 is further introduced, light source 3 with reference to specific device
Can be super continuous wide range laser, super continuous the wide range laser covering visible light wave band or near infrared light wave band, or both group
Close, can directly light or be conducted through incident optical 4.Displacement platform 6 is stepper motor, and spectrum acquisition device 7 is spectrometer, modulation
Device 9 is chopper, described below：
As shown in fig. 6, chopper 9, which modulates super continuous wide range laser 3, makes its sender's wave optical signal, super continuous wide range laser
3 are transmitted by incident optical 4 to the blood sample in blood bag 5, and spectrometer 7 passes through the acquisition of transmission spectrum of the output optical fiber 8, enters
Penetrate optical fiber 4 and be close to blood bag 5 respectively and coaxial with the output optical fiber 8, a positions are the first position of incident optical 4, spectrometer 7 is gathered
Incident optical 4 is with the output optical fiber 8 with respect to the transmitted spectrum under a of the first position；Incident light is then being ensured by stepper motor 6
Fibre 4 with the position of the output optical fiber 8 remain unchanged it is coaxial on the premise of, control incident optical 4 be moved at the b of the second place, spectrometer 7 is gathered
Transmitted spectrum under the incident optical 4 and relative second position b of the output optical fiber 8.
Wherein, transmitted spectrum, construction absorption spectrum, normalization, founding mathematical models, Yi Jiji in follow-up construction frequency domain
The step of calculating free hemoglobin content is same as Example 1, and the embodiment of the present invention is not repeated this.
The embodiment of the present invention is to the model of each device in addition to specified otherwise is done, and the model of other devices is not limited,
As long as the device of above-mentioned functions can be completed.
In summary, contain the embodiments of the invention provide free hemoglobin in a kind of pair of optical path modulation light source measurement blood bag
The method of amount, it is workable, the influence that spectral background noise, light source change and blood bag is brought is eliminated, free blood is improved
The analysis precision of Lactoferrin content, solves the problems, such as the Non-Destructive Testing of free hemoglobin content in blood bag, efficient, easy, nothing
Pollution.
Embodiment 10
As different from Example 9, light source 3 is bromine tungsten filament lamp wideband light source, the bromine tungsten filament lamp wideband light source 3 to the embodiment of the present invention
Covering visible light wave band or near infrared light wave band, or both combination, can directly light or be conducted through incident optical 4.
As shown in figure 5, the modulation bromine tungsten filament lamp of chopper 9 wideband light source 3 makes its sender's wave optical signal, bromine tungsten filament lamp wideband light source
3 are transmitted by incident optical 4 to the blood sample in blood bag 5, pass through the acquisition of transmission spectrum of the output optical fiber 8 by spectrometer 7,
Incident optical 4 is close to blood bag 5 and coaxial respectively with the output optical fiber 8, and the collection incident optical 4 of spectrometer 7 is relative with the output optical fiber 8 should
Transmitted spectrum under a of first position；Then ensureing that it is coaxial that incident optical 4 remains unchanged with the position of the output optical fiber 8 by stepper motor 6
On the premise of, control incident optical 4 is moved to the b of the second place at, and collection incident optical 4 and the output optical fiber 8 of spectrometer 7 is with respect to the
Transmitted spectrum under two position b.
Wherein, transmitted spectrum, construction absorption spectrum, normalization, founding mathematical models, Yi Jiji in follow-up construction frequency domain
The step of calculating free hemoglobin content is same as Example 1, and the embodiment of the present invention is not repeated this.
The embodiment of the present invention is to the model of each device in addition to specified otherwise is done, and the model of other devices is not limited,
As long as the device of above-mentioned functions can be completed.
In summary, contain the embodiments of the invention provide free hemoglobin in a kind of pair of optical path modulation light source measurement blood bag
The method of amount, it is workable, the influence that spectral background noise, light source change and blood bag is brought is eliminated, free blood is improved
The analysis precision of Lactoferrin content, solves the problems, such as the Non-Destructive Testing of free hemoglobin content in blood bag, efficient, easy, nothing
Pollution.
Embodiment 11
The embodiment of the present invention from unlike embodiment 9,10, light source 3 be xenon lamp wide spectrum light source, the xenon lamp wide spectrum light source 3
Covering visible light wave band or near infrared light wave band, or both combination, can directly light or be conducted through incident optical；Displacement is put down
Platform 6 is magnet attraction device, described below：
As shown in figure 5, the modulation xenon lamp of chopper 9 wide spectrum light source 3 makes its sender's wave optical signal, xenon lamp wide spectrum light source 3 leads to
Cross incident optical 4 to transmit blood sample in blood bag 5, spectrometer 7 passes through the acquisition of transmission spectrum of the output optical fiber 8, incident light
Fibre 4 is close to blood bag 5 and coaxial respectively with the output optical fiber 8, and the collection incident optical 4 of spectrometer 7 is relative this first with the output optical fiber 8
Put the transmitted spectrum under a；Then ensureing that it is coaxial that incident optical 4 remains unchanged with the position of the output optical fiber 8 by magnet attraction device 6
Under the premise of control incident optical 4 be moved to the b of the second place at, collection incident optical 4 and the output optical fiber 8 of spectrometer 7 is with respect to second
Transmitted spectrum under the b of position.
Wherein, transmitted spectrum, construction absorption spectrum, normalization, founding mathematical models, Yi Jiji in follow-up construction frequency domain
The step of calculating free hemoglobin content is same as Example 1, and the embodiment of the present invention is not repeated this.
The embodiment of the present invention is to the model of each device in addition to specified otherwise is done, and the model of other devices is not limited,
As long as the device of above-mentioned functions can be completed.
In summary, contain the embodiments of the invention provide free hemoglobin in a kind of pair of optical path modulation light source measurement blood bag
The method of amount, it is workable, the influence that spectral background noise, light source change and blood bag is brought is eliminated, free blood is improved
The analysis precision of Lactoferrin content, solves the problems, such as the Non-Destructive Testing of free hemoglobin content in blood bag, efficient, easy, nothing
Pollution.
Embodiment 12
The embodiment of the present invention from unlike above-described embodiment 9,10,11, light source 3 may be used also according to the need in practical application
Can also can also be adopted using other mobile devices, spectrum acquisition device 7 using the light source of other models, displacement platform 6
Use other reception devices.When implementing, the embodiment of the present invention is not limited to the model of above-mentioned device.
The embodiment of the present invention is to position a, a '；Position b, b ' and move mode etc. be not restricted, as long as this hair can be realized
The function of bright embodiment, within the protection domain of the application.
In summary, contain the embodiments of the invention provide free hemoglobin in a kind of pair of optical path modulation light source measurement blood bag
The method of amount, it is workable, the influence that spectral background noise, light source change and blood bag is brought is eliminated, free blood is improved
The analysis precision of Lactoferrin content, solves the problems, such as the Non-Destructive Testing of free hemoglobin content in blood bag, efficient, easy, nothing
Pollution.
Claims (10)
1. the method for free hemoglobin content in a kind of pair of optical path modulation light source measurement blood bag, it is characterised in that methods described
Comprise the following steps：
Light source light extraction optical port is close to blood bag with spectrum acquisition device entrance slit, and modulating device modulated light source makes its sender's glistening light of waves
Signal, light source is transmitted to blood sample, spectrum acquisition device acquisition of transmission spectrum；
Displacement platform is under the premise of ensureing that light source light extraction optical port and spectrum acquisition device entrance slit are coaxial, control light source movement,
By spectrum acquisition device acquisition of transmission spectrum；
Transmitted spectrum at two positions is transformed into transmitted spectrum in frequency domain construction frequency domain respectively, by transmitted spectrum in two frequency domains
Each wavelength under light intensity ratio ask logarithm to be absorbed spectrum, by after absorption spectrum normalized with existing chemical analysis
Comparative result, founding mathematical models；
The transmitted spectrum at unknown blood two positions is gathered, two transmitted spectrums are transformed into transmitted light in frequency domain construction frequency domain respectively
Spectrum, asks logarithm to be absorbed spectrum light intensity ratio under each wavelength of transmitted spectrum in two frequency domains, absorption spectrum is carried out
Normalization, and bring mathematical modeling calculating into, obtain the content of free hemoglobin；
Methods described changes light path by command displacement platform, in the blood bag that different light path strong points gather under same modulated light source
The transmitted spectrum of blood, eliminates the influence that light source change and blood bag are brought, eliminates the influence of spectral background noise, improve free blood
The analysis precision of Lactoferrin content, solves the Non-Destructive Testing of free hemoglobin content in blood bag.
2. the method for free hemoglobin in a kind of pair of optical path modulation light source measurement blood bag according to claim 1, it is special
Levy and be, be specially the step of transmitted spectrum in the construction frequency domain：
Modulating device by modulation of source into square wave optical signal, by spectrum acquisition device acquisition of transmission spectrum, by the every of transmitted spectrum
The time series of individual wavelength transforms to frequency domain, with transmitted spectrum in the fundametal compoment construction frequency domain of each wavelength.
3. the method for free hemoglobin content in a kind of pair of optical path modulation light source measurement blood bag according to claim 1,
Characterized in that, control light source movement, by spectrum acquisition device acquisition of transmission spectrum the step of be specially：
Light source is transmitted at a of position to blood sample, by spectrum acquisition device acquisition of transmission spectrum；
Displacement platform control light source is moved to position b, by spectrum acquisition device acquisition of transmission spectrum；
Or,
Light source is transmitted to the blood sample in blood bag, by spectrum acquisition device in position a acquisition of transmission spectrum；
The transmitted spectrum that displacement platform control spectrum acquisition device is moved at position b, collection position b；
Or,
The blood sample in blood bag is transmitted by light source at a of position, gathers saturating by spectrum acquisition device at position a ' places
Penetrate spectrum；
Displacement platform controls light source and spectrum acquisition device to be respectively moved to position b, b ' place, by spectrum acquisition device acquisition of transmission
Spectrum.
4. the side of free hemoglobin content in a kind of pair of optical path modulation light source measurement blood bag according to claim 1 or 2
Method, it is characterised in that methods described also includes：
One optical fiber is set at light source, as incident optical, and ensures that incident optical is close to spectrum acquisition device entrance slit
Blood bag and coaxial；
Or,
One optical fiber is set at spectrum acquisition device, as the output optical fiber, and ensures that the output optical fiber is close to light source light extraction optical port
Blood bag and coaxial；
Or,
Incident optical and the output optical fiber are set respectively at light source and spectrum acquisition device, and ensure incident optical and the output optical fiber
It is close to blood bag and coaxial.
5. the method for free hemoglobin content in a kind of pair of optical path modulation light source measurement blood bag according to claim 3,
Characterized in that,
A positions are the first position of incident optical, are gathered by spectrum acquisition device under incident optical and the output optical fiber relative position a
Transmitted spectrum；On the premise of guarantee incident optical and the output optical fiber are coaxial, control incident optical is moved at the b of position, by
Spectrum acquisition device gathers incident optical and the transmitted spectrum under the output optical fiber relative position b.
6. the method for free hemoglobin content in a kind of pair of optical path modulation light source measurement blood bag according to claim 3,
Characterized in that,
A positions are the first position of the output optical fiber, are gathered by spectrum acquisition device under incident optical and the output optical fiber relative position a
Transmitted spectrum；On the premise of guarantee the output optical fiber and the output optical fiber are coaxial, control the output optical fiber is moved at the b of position, by
Spectrum acquisition device gathers incident optical and the transmitted spectrum under the output optical fiber relative position b.
7. the method for free hemoglobin content in a kind of pair of optical path modulation light source measurement blood bag according to claim 3,
Characterized in that,
A, a ' be respectively incident optical and the output optical fiber first position, incident optical and emergent light are gathered by spectrum acquisition device
Transmitted spectrum under relative position a, a ' of fibre；On the premise of guarantee incident optical and the output optical fiber are coaxial, incident light is controlled
Fine and the output optical fiber is moved to position b, b ' place, incident optical and the output optical fiber relative position b, b are gathered by spectrum acquisition device '
Under transmitted spectrum.
8. the method for free hemoglobin content in a kind of pair of optical path modulation light source measurement blood bag according to claim 3,
Characterized in that, the light source is super continuous wide range laser, xenon lamp wide spectrum light source or bromine tungsten filament lamp wideband light source, the super continuous wide range
Laser, xenon lamp wide spectrum light source or bromine tungsten filament lamp wideband light source covering visible light wave band or near infrared light wave band, or both combination,
It can directly light or be conducted through incident optical.
9. the method for free hemoglobin content in a kind of pair of optical path modulation light source measurement blood bag according to claim 3,
Characterized in that, institute's translation stage is stepper motor or magnet attraction device；The spectrum acquisition device is spectrometer；It is described
Modulating device is chopper.
10. dissociate blood in a kind of pair of optical path modulation light source measurement blood bag according to any claim in claim 1-3
The method of Lactoferrin content, it is characterised in that
The mathematical modeling utilizes principal component analysis, artificial neural network, PLS, SVMs, signal point
Analysis or statistical method are set up.
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