CN107421903A - The method of double light path and multiposition modulated light source measurement free hemoglobin contents - Google Patents
The method of double light path and multiposition modulated light source measurement free hemoglobin contents Download PDFInfo
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- CN107421903A CN107421903A CN201710529567.1A CN201710529567A CN107421903A CN 107421903 A CN107421903 A CN 107421903A CN 201710529567 A CN201710529567 A CN 201710529567A CN 107421903 A CN107421903 A CN 107421903A
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
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Abstract
The invention discloses a kind of double light paths and the method for multiposition modulated light source measurement free hemoglobin content, including：The light extraction optical port of light source is close to blood bag with the entrance slit of spectrum acquisition device, and modulating device is modulated to light source, and light source transmits to blood sample, spectrum acquisition device acquisition of transmission spectrum；Displacement platform control light source is moved to different positions and changes light path measurement, the transmitted spectrum gathered under two light paths of each opening position is converted into transmitted spectrum in frequency domain construction frequency domain, and logarithm is asked to obtain the absorption spectrum under the position ratio of transmitted spectrum in two frequency domains of each opening position, by the absorption spectrum normalized of multiple opening positions, with reference to the data of chemical analysis, founding mathematical models；Same method gathers the absorption spectrum of the multiple opening positions of unknown blood sample, brings mathematical modeling into after normalization, obtains the content of free hemoglobin.
Description
Technical field
The present invention relates to spectrum complicated solution concentration analysis stoichiometry field, more particularly to a kind of double light paths and multiposition
The method that modulated light source measures free hemoglobin content.
Background technology
In the prior art, more ripe technology is to detect containing for free hemoglobin in blood bag by chemical analysis
Amount, there are the high outstanding advantages of accuracy, but the mode of chemical analysis can not meet quick, non-contact and free of contamination need
Ask, spectral measurement is also possible to realize the content detection of free hemoglobin in blood bag due to its non-contact, free of contamination characteristic.
In spectral detection, according to Lambert-Beer's law：The incident intensity I of each wavelength is measured respectively0And output intensity
I, the absorbance A of each wavelength is calculated by formula (1).∈ is material absorptivity under a certain wavelength, and c is the dense of material
Degree, b is optical path length.
In fact, fail to measure incident intensity I for various reasons0, such as：Incident intensity I0It is difficult to measure by force very much, but
If in incident intensity I0It is basicly stable it is constant in the case of, only measure an output intensity I can also obtain good result.But
The influence that spectral detection is influenceed and measured container by spectral background noise, light source change is difficult to the essence for reaching measurement needs
Degree.
The influence of light source is mainly shown as the change of spatial distribution and light intensity.The reason for causing light source to change, has a lot, such as
Light source voltage change, filament aging, variation of ambient temperature etc..In spectrum analysis, rarely have document to introduce light source to measurement accuracy
Influence, and reduce the intensity of light source change on measurement accuracy influence method.In research in the early time, with the mode of calibration come
Some interference are eliminated, are such as calibrated with water, but because light intensity is too strong, are difficult to operate in practice.Also have in many scholars' utilizations
Property attenuator or optical fibre light splitting mode measure incident intensity I0.By taking neutral filter as an example (following discussion unless stated otherwise,
Discussed on some wavelength), the output intensity I that measurement passes through neutral filtern, then the light intensity I of light source0 nAbsorbance can be used
A and output intensity InTo represent：
Then sample is replaced into neutral filter, measures the output intensity I of sampleS,
NoticeSo
Do not have in the final result of formula (4)(namely) occur, illustrate that the intensity (and its spectrum) of light source does not interfere with
Measurement to sample, as long as all measurements are all calibrated using same neutral attenuator, that is, keep lgIn+AnFor constant constant.
But different occasions are difficult to find duplicate neutral filter, and it is difficult to ensure that the position of sample and neutral filter
Put consistent.And due to the scattering of blood, the spectrum for causing to obtain has non-linear, and spectral background can not be completely eliminated
The influence of noise, for this problem, this patent proposes a kind of double light paths and the free blood red egg of multiposition modulated light source measurement
Bai Hanliang method.
The content of the invention
The invention provides a kind of double light paths and multiposition modulated light source measurement free hemoglobin content method, not only
The influence that spectral background noise, light source change and blood bag are brought, and the influence for greatly inhibiting blood scattered band are eliminated,
Improve the precision of free hemoglobin content analysis；Solve the problems, such as the Non-Destructive Testing of free hemoglobin content in blood bag,
Efficiently, it is easy, pollution-free, it is described below：
A kind of method of double light paths and multiposition modulated light source measurement free hemoglobin content, methods described are used to measure
Blood free hemoglobin content in blood bag, the described method comprises the following steps：
The light extraction optical port of light source, it is close to blood bag with the entrance slit of spectrum acquisition device, modulating device modulated light source makes it
Sender's wave optical signal, light source transmit to blood sample, spectrum acquisition device acquisition of transmission spectrum；
Displacement platform control light source is moved to diverse location, changes light path transmission blood on each position, by spectrum
Reception device acquisition of transmission spectrum, and transformed to transmitted spectrum in frequency domain construction frequency domain, two frequency domains of each opening position
Interior transmitted spectrum ratio seeks the absorption spectra of blood that logarithm is the opening position, at the absorption spectrum normalization of multiple opening positions
Reason, with reference to the data of chemical analysis, founding mathematical models；
The transmitted spectrum under two light paths of the multiple opening positions of unknown blood sample is gathered, is converted into construction frequency domain in frequency domain
Interior transmitted spectrum, calculate and bring the normalization of the absorption spectrums of multiple positions into mathematical modeling after absorption spectrum and calculated, obtain
The content of free hemoglobin；
Transmitted spectrum eliminates spectral background influence of noise in methods described construction frequency domain, is measured using double light paths and eliminates light source
Change and the influence of blood bag, the transmitted spectrum obtained using multiposition measuring are increased the information content of blood all the components, suppress blood
The influence that liquid scattered band comes, improve the precision of free hemoglobin content analysis；Solves free hemoglobin content in blood bag
Non-Destructive Testing problem.
It is specially the step of transmitted spectrum in the construction frequency domain：
Modulating device into square wave optical signal, transmits modulation of source, by spectrum acquisition device acquisition of transmission to blood
Spectrum, the time series of each wavelength of transmitted spectrum is transformed into frequency domain, with the fundametal compoment construction frequency domain of each wavelength
Transmitted spectrum.
Wherein, displacement platform control light source is moved to diverse location, changes light path transmission blood on each position, by
The step of spectrum acquisition device acquisition of transmission spectrum is specially：
At a of position, displacement platform control light source is respectively under two light paths：Position a and position a ' are to blood sample
Transmitted, by spectrum acquisition device acquisition of transmission spectrum；
Displacement platform control light source is moved to position b, respectively under two light paths i.e.：Position b and position b ' are to blood sample
Product are transmitted, by spectrum acquisition device acquisition of transmission spectrum；
Displacement platform control light source is moved to position n always, respectively under two light paths i.e.：Position n and position n ' are to blood
Liquid sample is transmitted, by spectrum acquisition device acquisition of transmission spectrum；
Or,
At a of position, displacement platform control spectrum acquisition device is respectively spectrum acquisition device under two light paths：Position
A and position a ' places acquisition of transmission spectrum；
Displacement platform control spectrum acquisition device is moved to position b, respectively under two light paths i.e.：Position b and position b '
Locate acquisition of transmission spectrum；
Displacement platform control spectrum acquisition device is moved to position n always, respectively under two light paths i.e.：Position n and position
Put n ' places acquisition of transmission spectrum.
Methods described also includes：
One optical fiber is set at light source, as incident optical, and ensures the incident narrow of incident optical and spectrum acquisition device
Seam is close to blood bag；
Or,
One optical fiber is set at spectrum acquisition device, as the output optical fiber, and ensures the output optical fiber and light source light extraction optical port
It is close to blood bag；
Or,
Incident optical and the output optical fiber are set respectively at light source and spectrum acquisition device, and ensure incident optical and outgoing
Optical fiber is close to blood bag.
At a of position, light source is respectively incident optical under two light paths by incident optical：Position a and position a ' places
Blood sample is transmitted, by spectrum acquisition device acquisition of transmission spectrum；
Displacement platform control incident optical is moved at the b of position, and light source is by incident optical respectively in the opening position two
Under light path i.e.：Position b and position b ' places transmit to blood sample, by spectrum acquisition device acquisition of transmission spectrum；
Control incident optical is moved at the n of position always, and light source is by incident optical respectively in two light paths of the opening position
It is lower to be：Position n and position n ' places transmit to blood sample, by spectrum acquisition device acquisition of transmission spectrum.
Wherein, the output optical fiber is at a of position, by spectrum acquisition device by the output optical fiber under two light paths of the opening position
I.e.：Position a and position a ' places acquisition of transmission spectrum；
Displacement platform control the output optical fiber is moved at the b of position, and spectrum acquisition device is by the output optical fiber in the opening position
Under two light paths i.e.：Position b and position b ' places acquisition of transmission spectrum；
Control the output optical fiber is moved at the n of position always, and spectrum acquisition device is by the output optical fiber in the opening position two
Under light path i.e.：Position n and position n ' places acquisition of transmission spectrum.
Further, the light source is super continuous wide range laser, the super continuous wide range laser covering visible light wave band or near
Infrared band, or both combination, can directly light or be conducted through incident optical.
Further, institute's translation stage is stepper motor；The spectrum acquisition device is spectrometer；The modulating device
For chopper.
Further, the light source is xenon lamp wide spectrum light source or bromine tungsten filament lamp wide spectrum light source, the xenon lamp wide spectrum light source or bromine tungsten
Lamp wide spectrum light source covering visible light wave band or near infrared light wave band, or both combination, can directly light or be passed through incident optical
Lead.
Further, the mathematical modeling utilizes principal component analysis, artificial neural network, PLS, support
Vector machine, signal analysis or statistical method are established.
The beneficial effect of technical scheme provided by the invention is：
1st, the present invention changes position and light path by command displacement platform, is gathered under double light paths at diverse location same
The transmitted spectrum of blood in blood bag under modulated light source, and transmitted spectrum in frequency domain construction frequency domain is converted it to, so as to eliminate
The influence of transmitted spectrum ambient noise；
2nd, the influence that the present invention eliminates light source change using double light path measurements and blood bag is brought, and dissipating due to blood
Penetrating property, transmitted spectrum is caused to be scattered to multiple directions, and the transmitted spectrum that multiposition measuring obtains adds blood all the components
Information content, so as to greatly inhibit blood scattered band come influence；
3rd, the present invention improves the precision of free hemoglobin content analysis；Solves free hemoglobin content in blood bag
Non-Destructive Testing problem, it is efficient, easy, pollution-free.
Brief description of the drawings
Fig. 1 is the method schematic that double light paths and multiposition modulated light source measure free hemoglobin content；
Fig. 2 is double light path transmitted spectrum schematic diagrams；
Fig. 3 is the method schematic diagram that double light paths and multiposition modulated light source measure free hemoglobin content in embodiment 1；
Fig. 4, which is that the method for double light paths and multiposition modulated light source measurement free hemoglobin content is another in embodiment 2, to be shown
It is intended to；
Fig. 5, which is that the method for double light paths and multiposition modulated light source measurement free hemoglobin content is another in embodiment 3, to be shown
It is intended to；
Fig. 6, which is that the method for double light paths and multiposition modulated light source measurement free hemoglobin content is another in embodiment 4, to be shown
It is intended to；
Fig. 7, which is that the method for double light paths and multiposition modulated light source measurement free hemoglobin content is another in embodiment 5, to be shown
It is intended to；
Fig. 8, which is that the method for double light paths and multiposition modulated light source measurement free hemoglobin content is another in embodiment 6, to be shown
It is intended to.
In accompanying drawing, the list of parts representated by each label is as follows：
1：First light path； 2：Second light path；
3：Light source； 4：Incident optical；
5：Blood bag； 6：Displacement platform；
7：Spectrum acquisition device； 8：The output optical fiber；
9：Modulating device；
a、b…n；A ', b ' ... n ' are：It is close to the position of packaging bag.
Situation of the above-mentioned position in practical application is set, and need to ensure a and a '；B and b ' ... n and n ' positions are same
Axle.
Embodiment
To make the object, technical solutions and advantages of the present invention clearer, embodiment of the present invention is made below further
It is described in detail on ground.
When modulating device is positioned among light source optical path, light can periodically by with block.Now light source is sent
Light is modulated into the square wave optical signal with certain frequency.The frequency and light source for each wavelength of transmitted spectrum that transmission blood obtains
The square wave light frequency sent is consistent, and by taking a certain wavelength X of transmitted spectrum as an example, Fig. 1 is that the waveform of λ wavelength and spectrum acquisition device accumulate
Corresponding integrated value schematic diagram between timesharing, B are ambient noise, and T is the time of integration of spectrum acquisition device, and T1=T2=...=
Ti=T；T1It is ambient noise corresponding to the time of integration in section, the light intensity amplitude that now spectrum acquisition device receives is background
The integrated value of noise, numerical value minimum are designated as Imin；TiIt is the light intensity and ambient noise of λ wavelength corresponding to the time of integration in section, this
The light intensity amplitude that Shi Guangpu reception devices receive is the light intensity of λ wavelength and the integrated value of ambient noise, and numerical value maximum is designated as
Imax, T1With TiBetween the corresponding ambient noises of other times of integration part, another part corresponds to the light intensity of λ wavelength and background is made an uproar
Sound, therefore resulting integrated value is in (Imin, Imax) change in section.Thus, in T1~TiThe interior class value domain that can obtain is
(Imin, Imax) integration value sequence, it can be seen that, the integrated value of the wavelength is in (Imin, Imax) change in section and formed periodically
Signal, the integrated value of other wavelength is similar, and for strictly with cycle and synchronous cyclical signal.By to each wavelength
The time series of integrated value carry out Fourier transform, it is saturating in the frequency domain formed with the frequency domain fundametal compoment of all wavelengths integrated value
Spectrum is penetrated, ambient noise can be eliminated, increase substantially signal to noise ratio.
Double light path transmission spectrum methods are according to Lambert-Beer's law, as shown in Fig. 2 setting the first light path 1 and second respectively
Light path 2.Derivation is as follows：
Wherein, A1It is the absorbance of the first light path 1, A2It is the absorbance of the second light path 2.IOIt is the incident light of the first light path 1
Light intensity, while be also the second light path 2 incident light light intensity, I1It is the output intensity of the first light path 1, I2It is the second light path 2
Output intensity, b1It is the optical length of the first light path 1, b2It is the optical length of the second light path 2, Δ b is the difference of two optical lengths, ∈ extinctions
Coefficient is that c surveys material concentration.
The absorbance of double light path spectroscopic methodologies is still linear with optical path difference it can be seen from formula (7), meet lambert-
Beer law, and with light intensity of incident light IOIt is unrelated.Therefore, double light path methods are not influenceed by light source in theory, are deducted simultaneously
The influence of blood bag in itself.
This method eliminates the ambient noise of spectrum using transmitted spectrum in frequency domain is constructed；Eliminated using double light path measurements
The influence that light source changes and blood bag is brought；The transmitted spectrum obtained using multiposition measuring adds the information of blood all the components
Amount, the influence for greatly inhibiting blood scattered band.Improve the precision of free hemoglobin content analysis.Solve in blood bag
The Non-Destructive Testing problem of free hemoglobin content, it is efficient, easy, pollution-free.
Embodiment 1
The method of double light paths provided in an embodiment of the present invention and multiposition modulated light source measurement free hemoglobin content, institute
Using to device as shown in figure 3, including：Light source 3, blood bag 5, displacement platform 6, spectrum acquisition device 7 and modulating device 9.
Ensure that the light extraction optical port of light source 3 is close to blood bag 5 with the entrance slit of spectrum acquisition device 7, modulating device 9 modulates light
Source 3 makes its sender's wave optical signal, and light source 3 is under two light paths at a of position：Position a (corresponding first light path 1) and position
A ' (corresponding second light path 2) transmits to the blood sample in blood bag 5, by the acquisition of transmission spectrum of spectrum acquisition device 7；Then
Control light source 3 to be moved to position b by displacement platform 6, under two light paths at the b of position i.e.：Position b (corresponding first light paths
1) blood sample in blood bag 5 is transmitted with position b ' (corresponding second light path 2), by the acquisition of transmission of spectrum acquisition device 7
Spectrum；Control light source 3 to be moved to position n always by displacement platform 6, under two light paths at the n of position i.e.：Position n is (corresponding
First light path 1) and position n ' (corresponding second light path 2) blood sample in blood bag 5 is transmitted, by spectrum acquisition device 7
Acquisition of transmission spectrum.
The time series of each wavelength of transmitted spectrum under two light paths that each opening position is gathered transforms to frequency domain, with
The fundametal compoment of each wavelength constructs transmitted spectrum in frequency domain, and transmitted spectrum ratio seeks logarithm in two frequency domains of each opening position
The as absorption spectrum of the opening position blood, the absorption spectrum of the how individual positions of a, b ... n are normalized, method for normalizing
For：
Ag=A/max (A) (8)
In formula (8), AgTo normalize absorbance, max (A) is the absorbance maximum on different wave length, and A is extinction
Degree.With reference to the data of chemical analysis, principal component analysis (PCA, principal component analysis) or artificial is utilized
Neutral net (ANN, artificial neural network) or PLS (PLSR, particle least
Squares calibration analysis) or SVMs (SVM, support vector machines) signal point
The methods of analysis or statistics, can founding mathematical models.
The step of embodiment of the present invention is to specific founding mathematical models do not repeat, known to those skilled in the art.
Double light path transmitted spectrums under the how individual position of unknown blood sample a, b...n are gathered, convert it to frequency domain construction
Transmitted spectrum in frequency domain, transmitted spectrum ratio asks logarithm to be absorbed spectrum in two frequency domains of each opening position, by multiple positions
The absorption spectrum at the place of putting, which is normalized, brings the above-mentioned mathematical modeling established into, obtains the content of free hemoglobin.
To the model of each device in addition to specified otherwise is done, the model of other devices is not limited the embodiment of the present invention,
As long as the device of above-mentioned function can be completed.
In summary, the embodiments of the invention provide a kind of double light paths and multiposition modulated light source measurement free hemoglobin
The method of content, eliminate spectral background noise, the influence that light source change and packaging bag are brought, add blood all the components
Information content, the next influence of blood scattered band is greatly inhibited, improve the precision of free hemoglobin content analysis.Solves blood
The Non-Destructive Testing problem of free hemoglobin content, efficient, easy, pollution-free in bag.
Embodiment 2
The embodiment of the present invention differs only in embodiment 1, and the move mode of light source 3 and spectrum acquisition device 7 is not
Together, it is described below：
Referring to Fig. 4, ensure that the light extraction optical port of light source 3 is close to blood bag 5, modulation dress with the entrance slit of spectrum acquisition device 7
Putting 9 modulated light sources 3 makes its sender's wave optical signal, and light source 3 is transmitted to the blood sample in blood bag 5, filled by spectrum acquisition
7 are put to gather at a of position under double light paths i.e.：Position a and position a ' transmitted spectrum.Spectrum acquisition is controlled by displacement platform 6
Device 7 is moved to position b, is under the double light paths in collection position b places：Position b and position b ' transmitted spectrum；Pass through displacement platform 6
Control spectrum acquisition device 7 is moved to position n always, is under the double light paths in collection position n places：Position n and position n ' transmitted light
Spectrum.
Wherein, transmitted spectrum, construction absorption spectrum, normalization, founding mathematical models, Yi Jiji in follow-up construction frequency domain
The step of calculating free hemoglobin content is same as Example 1, and the embodiment of the present invention is not repeated this.
To the model of each device in addition to specified otherwise is done, the model of other devices is not limited the embodiment of the present invention,
As long as the device of above-mentioned function can be completed.
In summary, the embodiments of the invention provide a kind of double light paths and multiposition modulated light source measurement free hemoglobin
The method of content, eliminate spectral background noise, the influence that light source change and packaging bag are brought, add blood all the components
Information content, the next influence of blood scattered band is greatly inhibited, improve the precision of free hemoglobin content analysis.Solves blood
The Non-Destructive Testing problem of free hemoglobin content, efficient, easy, pollution-free in bag.
Embodiment 3
During specific implementation, due to the limitation of space structure, it is possible that light source 3 can not be close to spectrum acquisition device 7
The situation of blood bag 5, at this moment can be by setting an optical fiber, as incident optical 4 respectively at light source 3 and spectrum acquisition device 7
With the output optical fiber 8.
Referring to Fig. 5, the modulated light source 3 of modulating device 9 makes its sender's wave optical signal, and light source 3 is by incident optical 4 to blood bag
Blood sample in 5 is transmitted, and passes through the acquisition of transmission spectrum of the output optical fiber 8 by spectrum acquisition device 7, incident optical 4 is with going out
Penetrate optical fiber 8 and be close to blood bag 5 respectively, incident optical 4 is at a of position, and light source 3 is by incident optical 4 under the double light paths of the opening position
I.e.：Position a and position a ' transmit to blood sample, by the acquisition of transmission spectrum of spectrum acquisition device 7；Then put down by displacement
Platform 6 controls incident optical 4 to be moved at the b of position, and light source 3 is under the double light paths of the opening position by incident optical 4：Position b and
Position b ' transmits to blood sample, by the acquisition of transmission spectrum of spectrum acquisition device 7；Incident light is controlled by displacement platform 6
Fibre 4 is moved at the n of position always, and light source 3 is under the double light paths of the opening position by incident optical 4：Position n and position n ' are to blood
Liquid sample is transmitted, by the acquisition of transmission spectrum of spectrum acquisition device 7.
Wherein, transmitted spectrum, construction absorption spectrum, normalization, founding mathematical models, Yi Jiji in follow-up construction frequency domain
The step of calculating free hemoglobin content is same as Example 1, and the embodiment of the present invention is not repeated this.
To the model of each device in addition to specified otherwise is done, the model of other devices is not limited the embodiment of the present invention,
As long as the device of above-mentioned function can be completed.
In summary, the embodiments of the invention provide a kind of double light paths and multiposition modulated light source measurement free hemoglobin
The method of content, eliminate spectral background noise, the influence that light source change and packaging bag are brought, add blood all the components
Information content, the next influence of blood scattered band is greatly inhibited, improve the precision of free hemoglobin content analysis.Solves blood
The Non-Destructive Testing problem of free hemoglobin content, efficient, easy, pollution-free in bag.
Embodiment 4
The embodiment of the present invention is different only in that with embodiment 3, the output optical fiber 8, with position a, position b ... positions n；Position
A ', position b ' ... positions n ' setting are different, described below：
Referring to Fig. 6, the modulated light source 3 of modulating device 9 makes its sender's wave optical signal, and light source 3 is by incident optical 4 to blood bag
Blood sample in 5 is transmitted, and passes through the acquisition of transmission spectrum of the output optical fiber 8 by spectrum acquisition device 7, incident optical 4 is with going out
Penetrate optical fiber 8 and be close to blood bag 5 respectively, the output optical fiber 8 is gathered under the double light paths of the opening position at a of position by spectrum acquisition device 7
I.e.：Position a and position a ' transmitted spectrum；Then the output optical fiber 8 is controlled to be moved at the b of position by displacement platform 6, by spectrum
Reception device 7 is gathered under the double light paths of the opening position i.e.：Position b and position b ' transmitted spectrum；Controlled and be emitted by displacement platform 6
Optical fiber 8 is moved at the n of position always, is gathered by spectrum acquisition device 7 under the double light paths of the opening position i.e.：Position n's and position n '
Transmitted spectrum.
Wherein, transmitted spectrum, construction absorption spectrum, normalization, founding mathematical models, Yi Jiji in follow-up construction frequency domain
The step of calculating free hemoglobin content is same as Example 1, and the embodiment of the present invention is not repeated this.
To the model of each device in addition to specified otherwise is done, the model of other devices is not limited the embodiment of the present invention,
As long as the device of above-mentioned function can be completed.
In summary, the embodiments of the invention provide a kind of double light paths and multiposition modulated light source measurement free hemoglobin
The method of content, eliminate spectral background noise, the influence that light source change and packaging bag are brought, add blood all the components
Information content, the next influence of blood scattered band is greatly inhibited, improve the precision of free hemoglobin content analysis.Solves blood
The Non-Destructive Testing problem of free hemoglobin content, efficient, easy, pollution-free in bag.
Embodiment 5
As different from Example 3, the embodiment only includes incident optical 4 to the embodiment of the present invention, described below：
Referring to Fig. 7, the modulated light source 3 of modulating device 9 makes its sender's wave optical signal, and light source 3 is by incident optical 4 to blood bag
Blood sample in 5 is transmitted, by the acquisition of transmission spectrum of spectrum acquisition device 7, incident optical 4 and spectrum acquisition device 7
Entrance slit is close to blood bag 5 respectively, and incident optical 4 is at a of position, and light source 3 is by incident optical 4 under the double light paths of the opening position
I.e.：Position a and position a ' transmits to blood sample, by the acquisition of transmission spectrum of spectrum acquisition device 7；Then pass through displacement platform 6
Control incident optical 4 is moved at the b of position, and light source 3 is by incident optical 4 to being under the double light paths of the opening position：Position b and position
B ' transmits to blood sample, by the acquisition of transmission spectrum of spectrum acquisition device 7；The translation of incident optical 4 one is controlled by displacement platform 6
Move at the n of position, light source 3 is by incident optical 4 to being under the double light paths of the opening position：Position n and position n ' are saturating to blood sample
Penetrate, by the acquisition of transmission spectrum of spectrum acquisition device 7.
Wherein, transmitted spectrum, construction absorption spectrum, normalization, founding mathematical models, Yi Jiji in follow-up construction frequency domain
The step of calculating free hemoglobin content is same as Example 1, and the embodiment of the present invention is not repeated this.
To the model of each device in addition to specified otherwise is done, the model of other devices is not limited the embodiment of the present invention,
As long as the device of above-mentioned function can be completed.
, can also be according to the needs in practical application, to position a, position b ... positions n during specific implementation；Position a ', position
The position n ' that puts b ' ... and mobile mode are set, i.e., can also include a variety of embodiments, the embodiment of the present invention pair
This is not limited.
In summary, the embodiments of the invention provide a kind of double light paths and multiposition modulated light source measurement free hemoglobin
The method of content, eliminate spectral background noise, the influence that light source change and packaging bag are brought, add blood all the components
Information content, the next influence of blood scattered band is greatly inhibited, improve the precision of free hemoglobin content analysis.Solves blood
The Non-Destructive Testing problem of free hemoglobin content, efficient, easy, pollution-free in bag.
Embodiment 6
As different from Example 3, the embodiment only includes the output optical fiber 8 to the embodiment of the present invention, described below：
Referring to Fig. 8, the modulated light source 3 of modulating device 9 makes its sender's wave optical signal, and light source 3 is to the blood sample in blood bag 5
Transmitted, the acquisition of transmission spectrum of the output optical fiber 8, light extraction optical port and the output optical fiber 8 of light source 3 are passed through by spectrum acquisition device 7
It is close to blood bag 5 respectively, the output optical fiber 8 is gathered under the double light paths of the opening position i.e. at a of position by spectrum acquisition device 7：Position a
With position a ' transmitted spectrum；Then the output optical fiber 8 is controlled to be moved at the b of position by displacement platform 6, by spectrum acquisition device
7 gather under the double light paths of the opening position i.e.：Position b and position b ' transmitted spectrum；The output optical fiber 8 one is controlled by displacement platform 6
Straight move into place is put at n, is gathered by spectrum acquisition device 7 under the double light paths of the opening position i.e.：Position n and position n ' transmitted light
Spectrum.
Wherein, transmitted spectrum, construction absorption spectrum, normalization, founding mathematical models, Yi Jiji in follow-up construction frequency domain
The step of calculating free hemoglobin content is same as Example 1, and the embodiment of the present invention is not repeated this.
, can also be according to the needs in practical application, to position a, position b ... positions n during specific implementation；Position a ', position
The position n ' that puts b ' ... and mobile mode are set, i.e., can also include a variety of embodiments.
To the model of each device in addition to specified otherwise is done, the model of other devices is not limited the embodiment of the present invention,
As long as the device of above-mentioned function can be completed.
In summary, the embodiments of the invention provide a kind of double light paths and multiposition modulated light source measurement free hemoglobin
The method of content, eliminate spectral background noise, the influence that light source change and packaging bag are brought, add blood all the components
Information content, the next influence of blood scattered band is greatly inhibited, improve the precision of free hemoglobin content analysis.Solves blood
The Non-Destructive Testing problem of free hemoglobin content, efficient, easy, pollution-free in bag.
Embodiment 7
Select, the scheme in above-described embodiment 1-6 is further introduced, light source can with reference to specific device
Think super continuous wide range laser, the super continuous wide range laser covering visible light wave band or near infrared light wave band, or both group
Close, can directly light or be conducted through incident optical 4.Displacement platform 6 is stepper motor, and spectrum acquisition device 7 is spectrometer, modulation
Device 9 is chopper, described below：
Referring to Fig. 5, chopper 9, which modulates super continuous wide range laser 3, makes its sender's wave optical signal, super continuous wide range laser 3
The blood sample in blood bag 5 is transmitted by incident optical 4, the acquisition of transmission spectrum of the output optical fiber 8 is passed through by spectrometer 7,
Incident optical 4 is close to blood bag 5 respectively with the output optical fiber 8, and for incident optical 4 at a of position, super continuous wide range laser 3 passes through incidence
Optical fiber 4 under the double light paths of the opening position i.e.：Position a and position a ' transmit to blood sample, by the acquisition of transmission light of spectrometer 7
Spectrum；Then incident optical 4 is controlled to be moved at the b of position by stepper motor 6, super continuous wide range laser 3 is existed by incident optical 4
Under the double light paths of the opening position i.e.：Position b and position b ' transmit to blood sample, by the acquisition of transmission spectrum of spectrometer 7；Stepping
Motor 6 controls incident optical 4 to be moved to always at the n of position, and super continuous wide range laser 3 is double in the opening position by incident optical 4
Under light path i.e.：Position n and position n ' transmit to blood sample, by the acquisition of transmission spectrum of spectrometer 7.
Wherein, transmitted spectrum, construction absorption spectrum, normalization, founding mathematical models, Yi Jiji in follow-up construction frequency domain
The step of calculating free hemoglobin content is same as Example 1, and the embodiment of the present invention is not repeated this.
To the model of each device in addition to specified otherwise is done, the model of other devices is not limited the embodiment of the present invention,
As long as the device of above-mentioned function can be completed.
In summary, the embodiments of the invention provide a kind of double light paths and multiposition modulated light source measurement free hemoglobin
The method of content, eliminate spectral background noise, the influence that light source change and packaging bag are brought, add blood all the components
Information content, the next influence of blood scattered band is greatly inhibited, improve the precision of free hemoglobin content analysis.Solves blood
The Non-Destructive Testing problem of free hemoglobin content, efficient, easy, pollution-free in bag.
Embodiment 8
As different from Example 7, light source 3 is bromine tungsten filament lamp wideband light source to the embodiment of the present invention, the bromine tungsten filament lamp wideband light source 3
Covering visible light wave band or near infrared light wave band, or both combination, can directly light or be conducted through incident optical 4.
Referring to Fig. 5, chopper 9, which modulates bromine tungsten filament lamp wideband light source 3, makes its sender's wave optical signal, bromine tungsten filament lamp wideband light source 3
The blood sample in blood bag 5 is transmitted by incident optical 4, the acquisition of transmission spectrum of the output optical fiber 8 is passed through by spectrometer 7,
Incident optical 4 is close to blood bag 5 respectively with the output optical fiber 8, and for incident optical 4 at a of position, bromine tungsten filament lamp wideband light source 3 passes through incidence
Optical fiber 4 under the double light paths of the opening position i.e.：Position a and position a ' transmit to blood sample, by the acquisition of transmission light of spectrometer 7
Spectrum；Then incident optical 4 is controlled to be moved at the b of position by stepper motor 6, bromine tungsten filament lamp wideband light source 3 is existed by incident optical 4
Under the double light paths of the opening position i.e.：Position b and position b ' transmit to blood sample, by the acquisition of transmission spectrum of spectrometer 7；Stepping
Motor 6 controls incident optical 4 to be moved to always at the n of position, and bromine tungsten filament lamp wideband light source 3 is double in the opening position by incident optical 4
Under light path i.e.：Position n and position n ' transmit to blood sample, by the acquisition of transmission spectrum of spectrometer 7.
Wherein, transmitted spectrum, construction absorption spectrum, normalization, founding mathematical models, Yi Jiji in follow-up construction frequency domain
The step of calculating free hemoglobin content is same as Example 1, and the embodiment of the present invention is not repeated this.
To the model of each device in addition to specified otherwise is done, the model of other devices is not limited the embodiment of the present invention,
As long as the device of above-mentioned function can be completed.
In summary, the embodiments of the invention provide a kind of double light paths and multiposition modulated light source measurement free hemoglobin
The method of content, eliminate spectral background noise, the influence that light source change and packaging bag are brought, add blood all the components
Information content, the next influence of blood scattered band is greatly inhibited, improve the precision of free hemoglobin content analysis.Solves blood
The Non-Destructive Testing problem of free hemoglobin content, efficient, easy, pollution-free in bag.
Embodiment 9
For the embodiment of the present invention unlike embodiment 7,8, light source 3 is xenon lamp wide spectrum light source, and the xenon lamp wide spectrum light source covers
Lid visible light wave range or near infrared light wave band, or both combination, can directly light or be conducted through incident optical, it is as detailed below
Description：
Referring to Fig. 5, chopper 9, which modulates xenon lamp wide spectrum light source 3, makes its sender's wave optical signal, and xenon lamp wide spectrum light source 3 passes through
Incident optical 4 transmits to the blood sample in blood bag 5, passes through the acquisition of transmission spectrum of the output optical fiber 8 by spectrometer 7, incident
Optical fiber 4 is close to blood bag 5 respectively with the output optical fiber 8, and at a of position, xenon lamp wide spectrum light source 3 is existed incident optical 4 by incident optical 4
Under the double light paths of the opening position i.e.：Position a and position a ' transmit to blood sample, by the acquisition of transmission spectrum of spectrometer 7；Then
Incident optical 4 is controlled to be moved at the b of position by stepper motor 6, xenon lamp wide spectrum light source 3 is by incident optical 4 in the opening position
Under double light paths i.e.：Position b and position b ' transmit to blood sample, by the acquisition of transmission spectrum of spectrometer 7；Stepper motor 6 is controlled
Incident optical 4 processed is moved at the n of position always, and xenon lamp wide spectrum light source 3 is under the double light paths of the opening position by incident optical 4：
Position n and position n ' transmit to blood sample, by the acquisition of transmission spectrum of spectrometer 7.
Wherein, transmitted spectrum, construction absorption spectrum, normalization, founding mathematical models, Yi Jiji in follow-up construction frequency domain
The step of calculating free hemoglobin content is same as Example 1, and the embodiment of the present invention is not repeated this.
To the model of each device in addition to specified otherwise is done, the model of other devices is not limited the embodiment of the present invention,
As long as the device of above-mentioned function can be completed.
In summary, the embodiments of the invention provide a kind of double light paths and multiposition modulated light source measurement free hemoglobin
The method of content, eliminate spectral background noise, the influence that light source change and packaging bag are brought, add blood all the components
Information content, the next influence of blood scattered band is greatly inhibited, improve the precision of free hemoglobin content analysis.Solves blood
The Non-Destructive Testing problem of free hemoglobin content, efficient, easy, pollution-free in bag.
Embodiment 10
For the embodiment of the present invention unlike above-described embodiment 7,8,9, needs of the light source 3 in practical application can be with
Other mobile devices can also be used using the light source of other models, displacement platform 6, spectrum acquisition device 7 can also use
Other reception devices.During specific implementation, the embodiment of the present invention is not limited to the model of above-mentioned device.
The embodiment of the present invention is to position a, position b ... positions n；Position a ', position b ' ... positions n ' and move mode etc. are equal
It is not restricted, as long as the function of the embodiment of the present invention can be realized, within the protection domain of the application.
In summary, the embodiments of the invention provide a kind of double light paths and multiposition modulated light source measurement free hemoglobin
The method of content, eliminate spectral background noise, the influence that light source change and packaging bag are brought, add blood all the components
Information content, the next influence of blood scattered band is greatly inhibited, improve the precision of free hemoglobin content analysis.Solves blood
The Non-Destructive Testing problem of free hemoglobin content, efficient, easy, pollution-free in bag.
Claims (10)
1. a kind of method of double light paths and multiposition modulated light source measurement free hemoglobin content, it is characterised in that the side
Method is used to measure the blood free hemoglobin content in blood bag, the described method comprises the following steps：
The light extraction optical port of light source, it is close to blood bag with the entrance slit of spectrum acquisition device, modulating device modulated light source sends it
Square wave optical signal, light source transmit to blood sample, spectrum acquisition device acquisition of transmission spectrum；
Displacement platform control light source is moved to diverse location, changes light path transmission blood on each position, by spectrum acquisition
Device acquisition of transmission spectrum, and transmitted spectrum in frequency domain construction frequency domain is transformed to, in two frequency domains of each opening position thoroughly
The absorption spectra of blood that spectral ratio asks logarithm to be the opening position is penetrated, by the absorption spectrum normalized of multiple opening positions,
With reference to the data of chemical analysis, founding mathematical models；
The transmitted spectrum under two light paths of the multiple opening positions of unknown blood sample is gathered, is converted into frequency domain in construction frequency domain thoroughly
Spectrum is penetrated, bringing the absorption spectrum normalization of multiple positions into mathematical modeling after calculating absorption spectrum is calculated, and is dissociated
The content of hemoglobin；
Transmitted spectrum eliminates spectral background influence of noise in methods described construction frequency domain, is measured using double light paths and eliminates light source change
With the influence of blood bag, the transmitted spectrum obtained using multiposition measuring increases the information content of blood all the components, suppresses blood and dissipates
The influence brought is penetrated, improves the precision of free hemoglobin content analysis；Free hemoglobin content is lossless in solution blood bag
Test problems.
2. the method for double light paths according to claim 1 and multiposition modulated light source measurement free hemoglobin content, its
It is characterised by, is specially the step of transmitted spectrum in the construction frequency domain：
Modulating device into square wave optical signal, transmits modulation of source to blood, by spectrum acquisition device acquisition of transmission spectrum,
The time series of each wavelength of transmitted spectrum is transformed into frequency domain, with transmitted light in the fundametal compoment construction frequency domain of each wavelength
Spectrum.
3. the side of a kind of double light paths according to claim 1 and multiposition modulated light source measurement free hemoglobin content
Method, it is characterised in that displacement platform control light source is moved to diverse location, changes light path transmission blood on each position,
It is specially by the step of spectrum acquisition device acquisition of transmission spectrum：
At a of position, displacement platform control light source is respectively under two light paths：Position a and position a ' is carried out to blood sample
Transmission, by spectrum acquisition device acquisition of transmission spectrum；
Displacement platform control light source is moved to position b, respectively under two light paths i.e.：Position b and position b ' enter to blood sample
Row transmission, by spectrum acquisition device acquisition of transmission spectrum；
Displacement platform control light source is moved to position n always, respectively under two light paths i.e.：Position n and position n ' are to blood sample
Product are transmitted, by spectrum acquisition device acquisition of transmission spectrum；
Or,
At a of position, displacement platform control spectrum acquisition device is respectively spectrum acquisition device under two light paths：Position a and
Position a ' places acquisition of transmission spectrum；
Displacement platform control spectrum acquisition device is moved to position b, respectively under two light paths i.e.：Position b and position b ' places are adopted
Collect transmitted spectrum；
Displacement platform control spectrum acquisition device is moved to position n always, respectively under two light paths i.e.：Position n and position n '
Locate acquisition of transmission spectrum.
4. a kind of double light paths according to claim 1 or 2 and multiposition modulated light source measure free hemoglobin content
Method, it is characterised in that methods described also includes：
One optical fiber is set at light source, as incident optical, and ensures that the entrance slit of incident optical and spectrum acquisition device is tight
Paste blood bag；
Or,
One optical fiber is set at spectrum acquisition device, as the output optical fiber, and ensures that the output optical fiber is close to light source light extraction optical port
Blood bag；
Or,
Incident optical and the output optical fiber are set respectively at light source and spectrum acquisition device, and ensure incident optical and the output optical fiber
It is close to blood bag.
5. the side of a kind of double light paths according to claim 3 and multiposition modulated light source measurement free hemoglobin content
Method, it is characterised in that
At a of position, light source is respectively incident optical under two light paths by incident optical：Position a and position a ' places are to blood
Liquid sample is transmitted, by spectrum acquisition device acquisition of transmission spectrum；
Displacement platform control incident optical is moved at the b of position, and light source is by incident optical respectively in two light paths of the opening position
It is lower to be：Position b and position b ' places transmit to blood sample, by spectrum acquisition device acquisition of transmission spectrum；
Control incident optical is moved at the n of position always, and light source is respectively under two light paths of the opening position by incident optical：
Position n and position n ' places transmit to blood sample, by spectrum acquisition device acquisition of transmission spectrum.
6. the side of a kind of double light paths according to claim 3 and multiposition modulated light source measurement free hemoglobin content
Method, it is characterised in that
The output optical fiber at a of position, by spectrum acquisition device by the output optical fiber under two light paths of the opening position i.e.：Position a
With position a ' places acquisition of transmission spectrum；
Displacement platform control the output optical fiber is moved at the b of position, and spectrum acquisition device is by the output optical fiber in the opening position two
Under light path i.e.：Position b and position b ' places acquisition of transmission spectrum；
Control the output optical fiber is moved at the n of position always, and spectrum acquisition device is by the output optical fiber in two light paths of the opening position
It is lower to be：Position n and position n ' places acquisition of transmission spectrum.
7. the side of a kind of double light paths according to claim 3 and multiposition modulated light source measurement free hemoglobin content
Method, it is characterised in that the light source is super continuous wide range laser, the super continuous wide range laser covering visible light wave band or near red
Outer optical band, or both combination, can directly light or be conducted through incident optical.
8. the side of a kind of double light paths according to claim 3 and multiposition modulated light source measurement free hemoglobin content
Method, it is characterised in that institute's translation stage is stepper motor；The spectrum acquisition device is spectrometer；The modulating device is
Chopper.
9. the side of a kind of double light paths according to claim 3 and multiposition modulated light source measurement free hemoglobin content
Method, it is characterised in that the light source is xenon lamp wide spectrum light source or bromine tungsten filament lamp wide spectrum light source, and the xenon lamp wide spectrum light source or bromine tungsten filament lamp are wide
Compose light source covering visible light wave band or near infrared light wave band, or both combination, can directly light or be conducted through incident optical.
10. a kind of double light paths and the measurement of multiposition modulated light source according to any claim in claim 1-3 are free
The method of content of hemoglobin, it is characterised in that
The mathematical modeling utilizes principal component analysis, artificial neural network, PLS, SVMs, signal point
Analysis or statistical method are established.
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