CN107421925A - Multiposition modulation transmission and the method for fluorescence excitation light source measurement free hemoglobin - Google Patents
Multiposition modulation transmission and the method for fluorescence excitation light source measurement free hemoglobin Download PDFInfo
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- CN107421925A CN107421925A CN201710529555.9A CN201710529555A CN107421925A CN 107421925 A CN107421925 A CN 107421925A CN 201710529555 A CN201710529555 A CN 201710529555A CN 107421925 A CN107421925 A CN 107421925A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6491—Measuring fluorescence and transmission; Correcting inner filter effect
Abstract
The invention discloses a kind of multiposition modulation transmission and the method for fluorescence excitation light source measurement free hemoglobin, including：Modulating device is modulated to light source, light source includes transmitted light source and fluorescence excitation light source, transmitted light source transmits to blood sample, fluorescence excitation light source excites to blood sample, displacement platform controls transmitted light source and fluorescence excitation light source to be moved to multiple positions and blood sample is transmitted and excited respectively, by spectrum acquisition device acquisition of transmission spectrum and fluorescence spectrum；By multiple station acquisitions to transmitted spectrum and fluorescence spectrum in the time series of each wavelength transform to frequency domain, with the fluorescence spectrum in the transmitted spectrum and frequency domain in the fundametal compoment construction frequency domain of each wavelength, the data of chemical analysis, founding mathematical models are combined after normalized；Same method gathers in the frequency domain of unknown multiple opening positions of blood sample fluorescence spectrum in transmitted spectrum and frequency domain, brings mathematical modeling into after normalization, obtains the content of free hemoglobin.
Description
Technical field
The present invention relates to spectrum complicated solution concentration analysis stoichiometry field, more particularly to a kind of multiposition that combines to modulate
Transmission and the method for fluorescence excitation light source measurement free hemoglobin.
Background technology
In existing technology, more ripe technology is to detect containing for free hemoglobin in blood bag by chemical analysis
Amount, there are the high outstanding advantages of accuracy, but the mode of chemical analysis can not meet quick, non-contact and free of contamination need
Ask, spectral measurement is also possible to realize the content detection of free hemoglobin in blood bag due to its non-contact, free of contamination characteristic.
For the complexity of blood constituent, simple transmitted spectrum specific aim is poor, further to improve the blood red egg that dissociates
White measurement accuracy, with reference to fluorescence it is with strong points the characteristics of, but strong scattering, fluorescence spectrum by blood be non-linear and light
The influence of ambient noise is composed, therefore the feature of surveyed material can not be reacted well.
The content of the invention
The invention provides a kind of multiposition modulation transmission and the method for fluorescence excitation light source measurement free hemoglobin, increase
The information content of free hemoglobin is added, has not only measured nonlinear problem with strong points, and greatly inhibiting spectrum, realize
Quickly, free hemoglobin composition high-acruracy survey in free of contamination blood bag.It is described below：
A kind of multiposition modulation transmission and the method for fluorescence excitation light source measurement free hemoglobin, methods described include with
Lower step：
The light extraction optical port of light source is close to blood bag with the entrance slit of spectrum acquisition device, and light source includes transmitted light source and fluorescence
Excitation source, modulating device modulated light source make its sender's wave optical signal, and transmitted light source transmits to blood sample, and fluorescence swashs
Luminous source excitation free hemoglobin produces fluorescence, spectrum acquisition device acquisition of transmission spectrum and fluorescence spectrum；
Displacement platform controls transmitted light source and fluorescence excitation light source to be moved to multiple positions, is gathered by spectrum acquisition device saturating
Penetrate spectrum and fluorescence spectrum；
The transmitted spectrum and fluorescence spectrum that multiple opening positions are collected transform to frequency domain, construct in frequency domain transmitted spectrum and
Fluorescence spectrum and normalized, the Comparative result with existing chemical analysis, founding mathematical models in frequency domain；
Gather unknown blood sample multiple opening positions transmitted spectrum and fluorescence spectrum, construct frequency domain in transmitted spectrum and
Mathematical modeling is brought into after fluorescence spectrum normalization, obtain the content of free hemoglobin in frequency domain；
The transmitted spectrum and fluorescence spectrum that methods described multiposition collects are same with free hemoglobin and other materials
Shi Xiangguan spectrum, increase free hemoglobin information content；It is non-linear to suppress spectrum；Eliminate spectral background noise；Realize it is quick,
Free hemoglobin composition high-acruracy survey in free of contamination blood bag.
It is specially the step of fluorescence spectrum in transmitted spectrum and frequency domain in the construction frequency domain：
Modulating device by modulation of source into square wave optical signal, by spectrum acquisition device acquisition of transmission spectrum and fluorescence spectrum,
The time series of each wavelength is transformed into frequency domain, with the transmitted spectrum and frequency domain in the fundametal compoment construction frequency domain of each wavelength
Interior fluorescence spectrum.
Wherein, displacement platform control transmitted light source and fluorescence excitation light source are moved to multiple positions, by spectrum acquisition device
The step of acquisition of transmission spectrum and fluorescence spectrum is specially：
Transmitted light source and fluorescence excitation light source are transmitted and excited respectively to blood sample at a of position, are connect by spectrum
Receiving apparatus acquisition of transmission spectrum and fluorescence spectrum；
Displacement platform controls transmitted light source and fluorescence excitation light source to be moved to position b, by spectrum acquisition device acquisition of transmission
Spectrum and fluorescence spectrum；
Displacement platform controls transmitted light source and fluorescence excitation light source to be moved to position n always, is gathered by spectrum acquisition device
Transmitted spectrum and fluorescence spectrum；
Or,
Transmitted light source and fluorescence excitation light source are transmitted and excited respectively to the blood sample in blood bag, by spectrum acquisition
Device acquisition of transmission spectrum and fluorescence spectrum at a of position；
Displacement platform control spectrum acquisition device is moved to position b, gathers transmitted spectrum and fluorescence spectrum at the b of position；
Displacement platform control spectrum acquisition device is moved to position n, gathers transmitted spectrum and fluorescence spectrum at the n of position.
Wherein, methods described also includes：
One optical fiber is set at light source, as incident optical, and ensures incident optical and spectrum acquisition device entrance slit
It is close to blood bag；
Or,
One optical fiber is set at spectrum acquisition device, as the output optical fiber, and ensures the output optical fiber and light source light extraction optical port
It is close to blood bag；
Or,
Incident optical and the output optical fiber are set respectively at light source and spectrum acquisition device, and ensure incident optical and outgoing
Optical fiber is close to blood bag.
Wherein, a positions are the first position of incident optical, and the transmitted light under the position is gathered by spectrum acquisition device
Spectrum and fluorescence spectrum；Displacement platform control incident optical is moved at the b of position, is gathered by spectrum acquisition device saturating under the position
Spectrum and fluorescence spectrum are penetrated, displacement platform control incident optical is moved at the n of position always, and the position is gathered by spectrum acquisition device
Transmitted spectrum and fluorescence spectrum under putting.
Wherein, a positions are the first position of the output optical fiber, and the transmitted light under the position is gathered by spectrum acquisition device
Spectrum and fluorescence spectrum；Displacement platform control the output optical fiber is moved at the b of position, is gathered by spectrum acquisition device saturating under the position
Spectrum and fluorescence spectrum are penetrated, displacement platform control the output optical fiber is moved at the n of position always, and the position is gathered by spectrum acquisition device
Transmitted spectrum and fluorescence spectrum under putting.
Further, the transmitted light source is super continuous wide range laser, the super continuous wide range laser covering visible light wave band
Or the combination of near infrared light wave band or both, fluorescence excitation light source are ultraviolet lamp, transmitted light source and fluorescence excitation light source can be straight
Sending and receiving light conducts through incident optical.
Further, institute's translation stage is stepper motor；The spectrum acquisition device is spectrometer；The modulating device
For chopper.
Further, the transmitted light source is xenon lamp wide spectrum light source or bromine tungsten filament lamp wide spectrum light source, covering visible light wave band or
Near infrared light wave band, or both combination, fluorescence excitation light source is ultraviolet laser tube or ultra-violet light-emitting pipe, transmitted light source and fluorescence
Excitation source can directly light or be conducted through incident optical.
Mathematical modeling described above utilizes principal component analysis, artificial neural network, PLS, supporting vector
Machine, signal analysis or statistical method are established.
The beneficial effect of technical scheme provided by the invention is：
1st, the transmitted spectrum and fluorescence spectrum of the invention by gathering blood in blood bag at diverse location, accordingly realization pair
The Non-Destructive Testing of free hemoglobin content in blood bag；
2nd, the present invention makes full use of in blood free hemoglobin to be produced the characteristic of fluorescence by ultraviolet excitation, but by
There is strong scattering in blood, and free hemoglobin is influenceed by other material concentrations simultaneously in blood, when ultraviolet light is by it
His material absorbing it is more, the ultraviolet light that can be received by free hemoglobin is fewer, therefore the spectrum for causing to obtain has very
Strong is non-linear；
3rd, the fluorescence spectrum that multiposition measuring obtains is the spectrum simultaneously related to free hemoglobin and other materials,
The information content of free hemoglobin is added with reference to transmitted spectrum, measurement is with strong points, greatly inhibits the non-linear of spectrum to ask
Topic, and fluorescence spectrum eliminates the influence of spectral background noise in transmitted spectrum and frequency domain in the frequency domain constructed, realize it is quick,
Free hemoglobin content high-acruracy survey in free of contamination blood bag.
Brief description of the drawings
Fig. 1 is the schematic diagram of the method for multiposition modulation transmission and fluorescence excitation light source measurement free hemoglobin；
Fig. 2 is the method signal that multiposition modulation transmission and fluorescence excitation light source measure free hemoglobin in embodiment 1
Figure；
Fig. 3 is that the method for multiposition modulation transmission and fluorescence excitation light source measurement free hemoglobin is another in embodiment 2
Schematic diagram；
Fig. 4 is that the method for multiposition modulation transmission and fluorescence excitation light source measurement free hemoglobin is another in embodiment 3
Schematic diagram；
Fig. 5 is that the method for multiposition modulation transmission and fluorescence excitation light source measurement free hemoglobin is another in embodiment 4
Schematic diagram；
Fig. 6 is that the method for multiposition modulation transmission and fluorescence excitation light source measurement free hemoglobin is another in embodiment 5
Schematic diagram；
Fig. 7 is that the method for multiposition modulation transmission and fluorescence excitation light source measurement free hemoglobin is another in embodiment 6
Schematic diagram.
In accompanying drawing, the list of parts representated by each label is as follows：
1：Light source：Including transmitted light source and fluorescence excitation light source； 2：Incident optical；
3：Blood bag； 4：Displacement platform；
5：Spectrum acquisition device； 6：The output optical fiber；
7：Modulating device； a：First position；
b：The second place； n：N-th position.
Embodiment
To make the object, technical solutions and advantages of the present invention clearer, embodiment of the present invention is made below further
It is described in detail on ground.
When modulating device is positioned among light source optical path, light can periodically by with block.Now light source is sent
Light is modulated into the square wave optical signal with certain frequency.The transmitted light and the frequency of fluorescence obtained through blood is sent with light source
Square wave light frequency it is consistent, by taking transmitted light λ wavelength as an example, Fig. 1 be λ wavelength waveforms it is corresponding with the spectrum acquisition device time of integration
Integrated value schematic diagram, B are ambient noise, and T is the time of integration of spectrum acquisition device, and T1=T2=...=Ti=T；T1Section
It is ambient noise corresponding to the interior time of integration, the light intensity amplitude that now spectrum acquisition device receives is the integration of ambient noise
Value, numerical value minimum are designated as Imin；TiIt is λ wavelength light intensity and ambient noise corresponding to the time of integration in section, now spectrum acquisition fills
It is λ wavelength light intensity and the integrated value of ambient noise to put the light intensity amplitude received, and numerical value maximum is designated as Imax, T1With TiBetween other
The time of integration corresponding ambient noise of a part, another part corresponds to λ wavelength light intensity and ambient noise, therefore resulting integration
Value is in (Imin, Imax) change in section.Thus, in T1~TiThe interior class value domain that can obtain is (Imin, Imax) integration value sequence,
As can be seen here, the integrated value of the wavelength is in (Imin, Imax) change in section and form cyclical signal, the transmitted light of other wavelength
Integrated value is similar, and for strictly with cycle and synchronous cyclical signal.Pass through the time of the integrated value to each wavelength
Sequence carries out Fourier transform, transmitted spectrum in the frequency domain formed with the frequency domain fundametal compoment of all wavelengths integrated value, can disappear
Except ambient noise, signal to noise ratio is increased substantially.Fluorescence and transmitted light are similarly.
The present invention, which solves traditional transmitted spectrum and conventional fluorescent spectrum, can not be completely eliminated the influence of spectral background noise,
Free hemoglobin in blood is made full use of to be produced the characteristic of fluorescence by ultraviolet excitation, but because blood has strong scattering
Property, and free hemoglobin is influenceed by other material concentrations simultaneously, when ultraviolet light is by the more of other material absorbings, can be swum
It is fewer from the ultraviolet light that hemoglobin receives, thus the spectrum for causing to obtain have it is very strong non-linear.Multiple position measurements
Obtained transmitted spectrum and fluorescence spectrum is the spectrum simultaneously related to free hemoglobin and other materials, therefore is added
The information content of free hemoglobin, measurement is with strong points, greatly inhibits the nonlinear problem of spectrum, and by constructing in frequency domain
Fluorescence spectrum eliminates the influence of spectral background noise in transmitted spectrum and frequency domain, realizes in quick, free of contamination blood bag and swims
From the high-acruracy survey of hemoglobin.
Embodiment 1
Multiposition modulation transmission provided in an embodiment of the present invention and the method for fluorescence excitation light source measurement free hemoglobin,
The device that uses as shown in Fig. 2 including：Light source 1, blood bag 3, displacement platform 4 and spectrum acquisition device 5 and modulation dress
Put 7.
Wherein, ensure that the light extraction optical port of light source 1 is close to blood bag 3, modulating device 7 with the entrance slit of spectrum acquisition device 5
Modulated light source 1 makes its sender's wave optical signal, and light source 1 is transmitted and swashed to the blood sample in blood bag 3 at a of first position
Hair, by the acquisition of transmission spectrum of spectrum acquisition device 5 and fluorescence spectrum acquisition of transmission spectrum and fluorescence spectrum；Pass through displacement platform 4
Control light source is moved to second place b, gathers the transmitted spectrum and fluorescence spectrum of the position；Light source is controlled by displacement platform 4
Position n is moved to always, gathers the transmitted spectrum and fluorescence spectrum of the position.
The transmitted spectrum and the time series of each wavelength of fluorescence spectrum that multiple opening positions are gathered transform to frequency domain, with
It is normalized in the fundametal compoment construction frequency domain of each wavelength in transmitted spectrum and frequency domain after fluorescence spectrum, normalization side
Method is：
Ag=A/max (A) (1)
In formula (1), AgTo normalize absorbance, max (A) is the absorbance maximum on different wave length, and A is extinction
Degree.With reference to the data of chemical analysis, principal component analysis (PCA, principal component analysis) or artificial is utilized
Neutral net (ANN, artificial neural network) or PLS (PLSR, particle least
Squares calibration analysis) or SVMs (SVM, support vector machines) signal point
The methods of analysis or statistics, can founding mathematical models.
The step of embodiment of the present invention is to specific founding mathematical models do not repeat, known to those skilled in the art.
The transmitted spectrum and fluorescence spectrum of the multiple positions of unknown blood sample are gathered, constructs transmitted spectrum and frequency domain in frequency domain
The above-mentioned mathematical modeling established is brought in normalization into after interior fluorescence spectrum, obtains the content of free hemoglobin.
To the model of each device in addition to specified otherwise is done, the model of other devices is not limited the embodiment of the present invention,
As long as the device of above-mentioned function can be completed.
In summary, the embodiments of the invention provide a kind of multiposition modulation transmission and the free blood of fluorescence excitation light source measurement
The method of Lactoferrin, not only eliminate the influence of spectral background noise, and the transmitted spectrum and fluorescence light that multiposition measuring obtains
Spectrum is the spectrum simultaneously related to free hemoglobin and other materials, adds the information content of free hemoglobin, measures pin
It is strong to property, the nonlinear problem of spectrum is greatly inhibited, it is high to realize free hemoglobin content in quick, free of contamination blood bag
Precision measure.
Embodiment 2
The embodiment of the present invention differs only in embodiment 1, and the move mode of light source 3 and spectrum acquisition device 7 is not
Together, it is described below：
Referring to Fig. 3, ensure that the light extraction optical port of light source 1 is close to blood bag 3 with the entrance slit of spectrum acquisition device 5, modulating device 7 is adjusted
Light source 1 processed makes its sender's wave optical signal, and light source 1 is transmitted and excited to the blood sample in blood bag 3, filled by spectrum acquisition
Put 5 acquisition of transmission spectrum and fluorescence spectrum.Control spectrum acquisition device 7 to be moved to second place b by displacement platform 6, gather
The transmitted spectrum and fluorescence spectrum of the opening position, then spectrum acquisition device 7 is controlled to be moved to position always by displacement platform 6
N, gather the transmitted spectrum and fluorescence spectrum of the opening position.
Wherein, fluorescence spectrum in transmitted spectrum and frequency domain in follow-up construction frequency domain, normalization, founding mathematical models, with
And the step of calculating free hemoglobin content is same as Example 1, the embodiment of the present invention is not repeated this.
To the model of each device in addition to specified otherwise is done, the model of other devices is not limited the embodiment of the present invention,
As long as the device of above-mentioned function can be completed.
In summary, the embodiments of the invention provide a kind of multiposition modulation transmission and the free blood of fluorescence excitation light source measurement
The method of Lactoferrin, not only eliminate the influence of spectral background noise, and the transmitted spectrum and fluorescence light that multiposition measuring obtains
Spectrum is the spectrum simultaneously related to free hemoglobin and other materials, adds the information content of free hemoglobin, measures pin
It is strong to property, the nonlinear problem of spectrum is greatly inhibited, it is high to realize free hemoglobin content in quick, free of contamination blood bag
Precision measure.
Embodiment 3
During specific implementation, due to the limitation of space structure, it is possible that light source 1 can not be close to spectrum acquisition device 5
The situation of blood bag 3, at this moment can be by setting an optical fiber, as incident optical 2 respectively at light source 1 and spectrum acquisition device 5
With the output optical fiber 6.
Referring to Fig. 4, the modulated light source 1 of modulating device 7 makes its sender's wave optical signal, and light source 1 is by incident optical 2 to blood bag
Blood sample in 3 is transmitted and excited, and passes through the acquisition of transmission spectrum of the output optical fiber 6 and fluorescence light by spectrum acquisition device 5
Acquisition of transmission spectrum and fluorescence spectrum are composed, incident optical 2 is close to blood bag 3 respectively with the output optical fiber 6, and a positions are incident optical 2
First position, spectrum acquisition device 5 gather the transmitted spectrum and fluorescence spectrum under the position；Incidence is controlled by displacement platform 4
Optical fiber 2 is moved at the b of the second place, and spectrum acquisition device 5 gathers the transmitted spectrum and fluorescence spectrum under the position, subsequent displacement
Platform 4 controls incident optical 2 to be moved to always at the n of position, and spectrum acquisition device 5 gathers the transmitted spectrum and fluorescence under the position
Spectrum.
Wherein, fluorescence spectrum in transmitted spectrum and frequency domain in follow-up construction frequency domain, normalization, founding mathematical models, with
And the step of calculating free hemoglobin content is same as Example 1, the embodiment of the present invention is not repeated this.
To the model of each device in addition to specified otherwise is done, the model of other devices is not limited the embodiment of the present invention,
As long as the device of above-mentioned function can be completed.
In summary, the embodiments of the invention provide a kind of multiposition modulation transmission and the free blood of fluorescence excitation light source measurement
The method of Lactoferrin, not only eliminate the influence of spectral background noise, and the transmitted spectrum and fluorescence light that multiposition measuring obtains
Spectrum is the spectrum simultaneously related to free hemoglobin and other materials, adds the information content of free hemoglobin, measures pin
It is strong to property, the nonlinear problem of spectrum is greatly inhibited, it is high to realize free hemoglobin content in quick, free of contamination blood bag
Precision measure.
Embodiment 4
The embodiment of the present invention is different only in that with embodiment 3, the output optical fiber 8, with first position a, second place b ... the
The setting of n positions is different, described below：
Referring to Fig. 5, the modulated light source 1 of modulating device 7 makes its sender's wave optical signal, and light source 1 is by incident optical 2 to blood bag
Blood sample in 3 is transmitted and excited, and passes through the acquisition of transmission spectrum of the output optical fiber 6 and fluorescence light by spectrum acquisition device 5
Acquisition of transmission spectrum and fluorescence spectrum are composed, incident optical 2 is close to blood bag 3 respectively with the output optical fiber 6, and a positions are the output optical fiber 6
First position, spectrum acquisition device 5 gather the transmitted spectrum and fluorescence spectrum under the position；Controlled and be emitted by displacement platform 4
Optical fiber 6 is moved at the b of the second place, and spectrum acquisition device 5 gathers the transmitted spectrum and fluorescence spectrum under the position, then passes through
Displacement platform 4 control the output optical fiber 6 be moved to always the n of position at, spectrum acquisition device 5 gather the position under transmitted spectrum with
Fluorescence spectrum.
Wherein, fluorescence spectrum in transmitted spectrum and frequency domain in follow-up construction frequency domain, normalization, founding mathematical models, with
And the step of calculating free hemoglobin content is same as Example 1, the embodiment of the present invention is not repeated this.
To the model of each device in addition to specified otherwise is done, the model of other devices is not limited the embodiment of the present invention,
As long as the device of above-mentioned function can be completed.
In summary, the embodiments of the invention provide a kind of multiposition modulation transmission and the free blood of fluorescence excitation light source measurement
The method of Lactoferrin, not only eliminate the influence of spectral background noise, and the transmitted spectrum and fluorescence light that multiposition measuring obtains
Spectrum is the spectrum simultaneously related to free hemoglobin and other materials, adds the information content of free hemoglobin, measures pin
It is strong to property, the nonlinear problem of spectrum is greatly inhibited, it is high to realize free hemoglobin content in quick, free of contamination blood bag
Precision measure.
Embodiment 5
As different from Example 3, the embodiment only includes incident optical 4 to the embodiment of the present invention, described below：
Referring to Fig. 6, the modulated light source 1 of modulating device 7 makes its sender's wave optical signal, and light source 1 is by incident optical 2 to blood bag
Blood sample in 3 is transmitted and excited, by the acquisition of transmission spectrum of spectrum acquisition device 5 and fluorescence spectrum acquisition of transmission spectrum
And fluorescence spectrum, incident optical 2 and the entrance slit of spectrum acquisition device 5 are close to blood bag 3 respectively, a positions are the of incident optical 2
One position, spectrum acquisition device 5 gather the transmitted spectrum and fluorescence spectrum under the position；Incident light is controlled by displacement platform 4
Fibre 4 is moved at the b of the second place, and spectrum acquisition device 5 gathers the transmitted spectrum and fluorescence spectrum under the position, then passes through position
Moving platform 4 controls incident optical 4 to be moved at the n of position, and spectrum acquisition device 5 gathers the transmitted spectrum and fluorescence light under the position
Spectrum.
Wherein, fluorescence spectrum in transmitted spectrum and frequency domain in follow-up construction frequency domain, normalization, founding mathematical models, with
And the step of calculating free hemoglobin content is same as Example 1, the embodiment of the present invention is not repeated this.
To the model of each device in addition to specified otherwise is done, the model of other devices is not limited the embodiment of the present invention,
As long as the device of above-mentioned function can be completed.
In summary, the embodiments of the invention provide a kind of multiposition modulation transmission and the free blood of fluorescence excitation light source measurement
The method of Lactoferrin, not only eliminate the influence of spectral background noise, and the transmitted spectrum and fluorescence light that multiposition measuring obtains
Spectrum is the spectrum simultaneously related to free hemoglobin and other materials, adds the information content of free hemoglobin, measures pin
It is strong to property, the nonlinear problem of spectrum is greatly inhibited, it is high to realize free hemoglobin content in quick, free of contamination blood bag
Precision measure.
Embodiment 6
As different from Example 3, the embodiment only includes the output optical fiber 6 to the embodiment of the present invention, described below：
Referring to Fig. 7, the modulated light source 1 of modulating device 7 makes its sender's wave optical signal, and light source 1 is to the blood sample in blood bag 3
Transmitted and excited, the acquisition of transmission spectrum of the output optical fiber 6 and fluorescence spectrum acquisition of transmission spectrum are passed through by spectrum acquisition device 5
And fluorescence spectrum, the light extraction optical port of light source 1 and the output optical fiber 6 are close to blood bag 3 respectively, a positions are the first position of light source 1, spectrum
Reception device 5 gathers the transmitted spectrum and fluorescence spectrum under the position；Light source 3 is controlled to be moved to second by displacement platform 4
Put at b, spectrum acquisition device 5 gathers light source 1 and transmitted spectrum and fluorescence spectrum under the position of the output optical fiber 6, then passes through
Displacement platform 4 controls light source 3 to be moved at the n of position always, and spectrum acquisition device 5 gathers the transmitted spectrum and fluorescence under the position
Spectrum.
Wherein, fluorescence spectrum in transmitted spectrum and frequency domain in follow-up construction frequency domain, normalization, founding mathematical models, with
And the step of calculating free hemoglobin content is same as Example 1, the embodiment of the present invention is not repeated this.
To the model of each device in addition to specified otherwise is done, the model of other devices is not limited the embodiment of the present invention,
As long as the device of above-mentioned function can be completed.
In summary, the embodiments of the invention provide a kind of multiposition modulation transmission and the free blood of fluorescence excitation light source measurement
The method of Lactoferrin, not only eliminate the influence of spectral background noise, and the transmitted spectrum and fluorescence light that multiposition measuring obtains
Spectrum is the spectrum simultaneously related to free hemoglobin and other materials, adds the information content of free hemoglobin, measures pin
It is strong to property, the nonlinear problem of spectrum is greatly inhibited, it is high to realize free hemoglobin content in quick, free of contamination blood bag
Precision measure.
Embodiment 7
Select, the scheme in above-described embodiment 1-6 is further introduced, light source 1 with reference to specific device
Including transmitted light source and fluorescence excitation light source, transmitted light source can be super continuous wide range laser, the super continuous wide range laser covering
Visible light wave range or near infrared light wave band, or both combination, excitation source is ultraviolet lamp, and above-mentioned light source can directly light
Or conducted through incident optical 2.Displacement platform 4 is stepper motor, and spectrum acquisition device 5 is spectrometer, and modulating device 7 is copped wave
Device, it is described below：
It is set to distinguish sender's wave optical signal as shown in figure 4, chopper 7 modulates super continuous wide range laser and ultraviolet lamp 1,
Super continuous wide range laser and ultraviolet lamp 1 are transmitted and excited to the blood sample in blood bag 3 by incident optical 2, by light
Spectrometer 5 passes through the acquisition of transmission spectrum of the output optical fiber 6 and fluorescence spectrum acquisition of transmission spectrum and fluorescence spectrum, and incident optical 2 is with going out
Penetrate optical fiber 6 and be close to blood bag 3 respectively, a positions are the first position of incident optical 2, and spectrometer 5 gathers the transmitted spectrum under the position
And fluorescence spectrum；Incident optical 2 is controlled to be moved at the b of the second place by stepper motor 4, spectrometer 5 is gathered under the position
Transmitted spectrum and fluorescence spectrum, subsequent stepper motor 4 control incident optical 2 to be moved to always at the n of position, and the collection of spectrometer 5 should
Transmitted spectrum and fluorescence spectrum under position.
Wherein, fluorescence spectrum in transmitted spectrum and frequency domain in follow-up construction frequency domain, normalization, founding mathematical models, with
And the step of calculating free hemoglobin content is same as Example 1, the embodiment of the present invention is not repeated this.
To the model of each device in addition to specified otherwise is done, the model of other devices is not limited the embodiment of the present invention,
As long as the device of above-mentioned function can be completed.
In summary, the embodiments of the invention provide a kind of multiposition modulation transmission and the free blood of fluorescence excitation light source measurement
The method of Lactoferrin, not only eliminate the influence of spectral background noise, and the transmitted spectrum and fluorescence light that multiposition measuring obtains
Spectrum is the spectrum simultaneously related to free hemoglobin and other materials, adds the information content of free hemoglobin, measures pin
It is strong to property, the nonlinear problem of spectrum is greatly inhibited, it is high to realize free hemoglobin content in quick, free of contamination blood bag
Precision measure.
Embodiment 8
As different from Example 7, transmitted light source is bromine tungsten filament lamp wide spectrum light source to the embodiment of the present invention, the bromine tungsten filament lamp wide spectrum optical
Source covering visible light wave band or near infrared light wave band, or both combination, fluorescence excitation light source is ultraviolet laser tube, above-mentioned light
Source 1 can directly light or be conducted through incident optical 2.
It is set to distinguish sender's glistening light of waves letter as shown in figure 4, chopper 7 modulates bromine tungsten filament lamp wide spectrum light source and ultraviolet laser tube 1
Number, bromine tungsten filament lamp wide spectrum light source and ultraviolet laser tube 1 are transmitted and excited to the blood sample in blood bag 3 by incident optical 2,
The acquisition of transmission spectrum of the output optical fiber 6 and fluorescence spectrum acquisition of transmission spectrum and fluorescence spectrum, incident optical 2 are passed through by spectrometer 5
It is close to blood bag 3 respectively with the output optical fiber 6, a positions are the first position of incident optical 2, and spectrometer 5 gathers the transmission under the position
Spectrum and fluorescence spectrum；Incident optical 2 is controlled to be moved at the b of the second place by stepper motor 4, spectrometer 5 gathers the position
Under transmitted spectrum and fluorescence spectrum, subsequent stepper motor 4 controls incident optical 2 to be moved to always at the n of position, stepper motor 5
Gather the transmitted spectrum and fluorescence spectrum under the position.
Wherein, fluorescence spectrum in transmitted spectrum and frequency domain in follow-up construction frequency domain, normalization, founding mathematical models, with
And the step of calculating free hemoglobin content is same as Example 1, the embodiment of the present invention is not repeated this.
To the model of each device in addition to specified otherwise is done, the model of other devices is not limited the embodiment of the present invention,
As long as the device of above-mentioned function can be completed.
In summary, the embodiments of the invention provide a kind of multiposition modulation transmission and the free blood of fluorescence excitation light source measurement
The method of Lactoferrin, not only eliminate the influence of spectral background noise, and the transmitted spectrum and fluorescence light that multiposition measuring obtains
Spectrum is the spectrum simultaneously related to free hemoglobin and other materials, adds the information content of free hemoglobin, measures pin
It is strong to property, the nonlinear problem of spectrum is greatly inhibited, it is high to realize free hemoglobin content in quick, free of contamination blood bag
Precision measure.
Embodiment 9
The embodiment of the present invention is unlike embodiment 7,8, and transmitted light source is xenon lamp wide spectrum light source, the xenon lamp wide spectrum light source
Covering visible light wave band or near infrared light wave band, or both combination, fluorescence excitation light source is ultra-violet light-emitting pipe, above-mentioned light source 1
It can directly light or be conducted through incident optical 2, it is described below：
It is set to distinguish sender's wave optical signal as shown in figure 4, chopper 7 modulates xenon lamp wide spectrum light source and ultra-violet light-emitting pipe 1,
Xenon lamp wide spectrum light source and ultra-violet light-emitting pipe 1 are transmitted and excited to the blood sample in blood bag 3 by incident optical 2, by light
Spectrometer 5 passes through the acquisition of transmission spectrum of the output optical fiber 6 and fluorescence spectrum acquisition of transmission spectrum and fluorescence spectrum, and incident optical 2 is with going out
Penetrate optical fiber 6 and be close to blood bag 3 respectively, a positions are the first position of incident optical 2, and spectrometer 5 gathers the transmitted spectrum under the position
And fluorescence spectrum；Incident optical 2 is controlled to be moved at the b of the second place by stepper motor 4, spectrometer 5 is gathered under the position
Transmitted spectrum and fluorescence spectrum, subsequent stepper motor 4 control incident optical 2 to be moved to always at the n of position, and the collection of spectrometer 5 should
Transmitted spectrum and fluorescence spectrum under position.
Wherein, fluorescence spectrum in transmitted spectrum and frequency domain in follow-up construction frequency domain, normalization, founding mathematical models, with
And the step of calculating free hemoglobin content is same as Example 1, the embodiment of the present invention is not repeated this.
To the model of each device in addition to specified otherwise is done, the model of other devices is not limited the embodiment of the present invention,
As long as the device of above-mentioned function can be completed.
In summary, the embodiments of the invention provide a kind of multiposition modulation transmission and the free blood of fluorescence excitation light source measurement
The method of Lactoferrin, not only eliminate the influence of spectral background noise, and the transmitted spectrum and fluorescence light that multiposition measuring obtains
Spectrum is the spectrum simultaneously related to free hemoglobin and other materials, adds the information content of free hemoglobin, measures pin
It is strong to property, the nonlinear problem of spectrum is greatly inhibited, it is high to realize free hemoglobin content in quick, free of contamination blood bag
Precision measure.
Embodiment 10
For the embodiment of the present invention unlike above-described embodiment 7,8,9, needs of the light source 1 in practical application can be with
Other mobile devices can also be used using the light source of other models, displacement platform 4, spectrum acquisition device 5 can also use
Other reception devices.During specific implementation, the embodiment of the present invention is not limited to the model of above-mentioned device.
The embodiment of the present invention is not restricted to first position a, second place b ... the n-th position n and move mode etc., only
The function of the embodiment of the present invention can be realized, within the protection domain of the application.
In summary, the embodiments of the invention provide a kind of multiposition modulation transmission and the free blood of fluorescence excitation light source measurement
The method of Lactoferrin, not only eliminate the influence of spectral background noise, and the transmitted spectrum and fluorescence light that multiposition measuring obtains
Spectrum is the spectrum simultaneously related to free hemoglobin and other materials, therefore adds the information content of free hemoglobin, is surveyed
Measure with strong points, greatly inhibit the nonlinear problem of spectrum, realize free hemoglobin in quick, free of contamination blood bag and contain
Measure high-acruracy survey.
Claims (10)
1. a kind of multiposition modulation transmission and the method for fluorescence excitation light source measurement free hemoglobin, it is characterised in that described
Method comprises the following steps：
The light extraction optical port of light source is close to blood bag with the entrance slit of spectrum acquisition device, and light source includes transmitted light source and fluorescence excitation
Light source, modulating device modulated light source make its sender's wave optical signal, and transmitted light source transmits to blood sample, fluorescent exciting
Source excitation free hemoglobin produces fluorescence, spectrum acquisition device acquisition of transmission spectrum and fluorescence spectrum；
Displacement platform controls transmitted light source and fluorescence excitation light source to be moved to multiple positions, by spectrum acquisition device acquisition of transmission light
Spectrum and fluorescence spectrum；
The transmitted spectrum and fluorescence spectrum that multiple opening positions are collected transform to frequency domain, construct transmitted spectrum and frequency domain in frequency domain
Interior fluorescence spectrum and normalized, the Comparative result with existing chemical analysis, founding mathematical models；
Transmitted spectrum and fluorescence spectrum of the unknown blood sample in multiple opening positions are gathered, constructs transmitted spectrum and frequency domain in frequency domain
Mathematical modeling is brought into after interior fluorescence spectrum normalization, obtains the content of free hemoglobin；
The transmitted spectrum and fluorescence spectrum that methods described multiposition collects be and free hemoglobin and the same phase of other materials
The spectrum of pass, increase free hemoglobin information content；It is non-linear to suppress spectrum；Eliminate spectral background noise；Realize quickly, without dirt
Free hemoglobin composition high-acruracy survey in the blood bag of dye.
2. a kind of multiposition modulation transmission according to claim 1 and the side of fluorescence excitation light source measurement free hemoglobin
Method, it is characterised in that be specially the step of fluorescence spectrum in transmitted spectrum and frequency domain in the construction frequency domain：
Modulating device by modulation of source into square wave optical signal, will be every by spectrum acquisition device acquisition of transmission spectrum and fluorescence spectrum
The time series of individual wavelength transforms to frequency domain, with glimmering in the transmitted spectrum and frequency domain in the fundametal compoment construction frequency domain of each wavelength
Light spectrum.
3. a kind of multiposition modulation transmission according to claim 1 and the side of fluorescence excitation light source measurement free hemoglobin
Method, it is characterised in that displacement platform controls transmitted light source and fluorescence excitation light source to be moved to multiple positions, by spectrum acquisition device
The step of acquisition of transmission spectrum and fluorescence spectrum is specially：
Transmitted light source and fluorescence excitation light source are transmitted and excited respectively to blood sample at a of position, are filled by spectrum acquisition
Put acquisition of transmission spectrum and fluorescence spectrum；
Displacement platform controls transmitted light source and fluorescence excitation light source to be moved to position b, by spectrum acquisition device acquisition of transmission spectrum
And fluorescence spectrum；
Displacement platform controls transmitted light source and fluorescence excitation light source to be moved to position n always, by spectrum acquisition device acquisition of transmission
Spectrum and fluorescence spectrum；
Or,
Transmitted light source and fluorescence excitation light source are transmitted and excited respectively to the blood sample in blood bag, by spectrum acquisition device
Acquisition of transmission spectrum and fluorescence spectrum at a of position；
Displacement platform control spectrum acquisition device is moved to position b, gathers transmitted spectrum and fluorescence spectrum at the b of position；
Displacement platform control spectrum acquisition device is moved to position n, gathers transmitted spectrum and fluorescence spectrum at the n of position.
4. a kind of multiposition modulation transmission according to claim 1 or 2 and fluorescence excitation light source measurement free hemoglobin
Method, it is characterised in that methods described also includes：
One optical fiber is set at light source, as incident optical, and ensures that incident optical is close to spectrum acquisition device entrance slit
Blood bag；
Or,
One optical fiber is set at spectrum acquisition device, as the output optical fiber, and ensures that the output optical fiber is close to light source light extraction optical port
Blood bag；
Or,
Incident optical and the output optical fiber are set respectively at light source and spectrum acquisition device, and ensure incident optical and the output optical fiber
It is close to blood bag.
5. a kind of multiposition modulation transmission according to claim 3 and the side of fluorescence excitation light source measurement free hemoglobin
Method, it is characterised in that
A positions are the first position of incident optical, and the transmitted spectrum and fluorescence spectrum under the position are gathered by spectrum acquisition device；
Displacement platform control incident optical is moved at the b of position, and the transmitted spectrum and fluorescence under the position are gathered by spectrum acquisition device
Spectrum, displacement platform control incident optical are moved at the n of position always, and the transmitted light under the position is gathered by spectrum acquisition device
Spectrum and fluorescence spectrum.
6. a kind of multiposition modulation transmission according to claim 3 and the side of fluorescence excitation light source measurement free hemoglobin
Method, it is characterised in that
A positions are the first position of the output optical fiber, and the transmitted spectrum and fluorescence spectrum under the position are gathered by spectrum acquisition device；
Displacement platform control the output optical fiber is moved at the b of position, and the transmitted spectrum and fluorescence under the position are gathered by spectrum acquisition device
Spectrum, displacement platform control the output optical fiber are moved at the n of position always, and the transmitted light under the position is gathered by spectrum acquisition device
Spectrum and fluorescence spectrum.
7. a kind of multiposition modulation transmission according to claim 3 and the side of fluorescence excitation light source measurement free hemoglobin
Method, it is characterised in that the transmitted light source is super continuous wide range laser, the super continuous wide range laser covering visible light wave band or
Near infrared light wave band, or both combination, fluorescence excitation light source is ultraviolet lamp, and transmitted light source and fluorescence excitation light source can be direct
Light or conducted through incident optical.
8. a kind of multiposition modulation transmission according to claim 3 and the side of fluorescence excitation light source measurement free hemoglobin
Method, it is characterised in that institute's translation stage is stepper motor；The spectrum acquisition device is spectrometer；The modulating device is
Chopper.
9. a kind of multiposition modulation transmission according to claim 3 and the side of fluorescence excitation light source measurement free hemoglobin
Method, it is characterised in that the transmitted light source is xenon lamp wide spectrum light source or bromine tungsten filament lamp wide spectrum light source, covering visible light wave band or near
Infrared band, or both combination, the fluorescence excitation light source is ultraviolet laser tube or ultra-violet light-emitting pipe, transmitted light source and glimmering
Phot-luminescence source can directly light or be conducted through incident optical.
10. a kind of multiposition modulation transmission and fluorescence excitation light source according to any claim in claim 1-3 are surveyed
The method for measuring free hemoglobin, it is characterised in that
The mathematical modeling utilizes principal component analysis, artificial neural network, PLS, SVMs, signal point
Analysis or statistical method are established.
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| US7899506B2 (en) * | 2002-11-04 | 2011-03-01 | Tianjin Sunshine Optics Technolies Co. Ltd. | Composite spectral measurement method and its spectral detection instrument |
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