CN107421924A - The method of free hemoglobin in multiposition modulation fluorescence excitation light source measurement blood bag - Google Patents
The method of free hemoglobin in multiposition modulation fluorescence excitation light source measurement blood bag Download PDFInfo
- Publication number
- CN107421924A CN107421924A CN201710529554.4A CN201710529554A CN107421924A CN 107421924 A CN107421924 A CN 107421924A CN 201710529554 A CN201710529554 A CN 201710529554A CN 107421924 A CN107421924 A CN 107421924A
- Authority
- CN
- China
- Prior art keywords
- fluorescence
- spectrum
- light source
- excitation light
- acquisition device
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6402—Atomic fluorescence; Laser induced fluorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6417—Spectrofluorimetric devices
Abstract
The invention discloses the method for free hemoglobin in a kind of multiposition modulation fluorescence excitation light source measurement blood bag, comprise the following steps：Modulating device is modulated to fluorescence excitation light source, the light extraction optical port of fluorescence excitation light source is close to packaging bag with the entrance slit of spectrum acquisition device, fluorescent exciting source excitation free hemoglobin produces fluorescence, displacement platform control fluorescence excitation light source is moved to multiple positions, and fluorescence spectrum is gathered by spectrum acquisition device；By multiple station acquisitions to fluorescence spectrum in the time series of each wavelength transform to frequency domain, with the fluorescence spectrum in the fundametal compoment construction frequency domain of each wavelength, the Comparative result after normalized with existing chemical analysis, founding mathematical models；Fluorescence spectrum in the frequency domain of the multiple opening positions of blood sample, brings mathematical modeling into after being normalized, obtains the content of free hemoglobin in the same unknown blood bag of method collection.
Description
Technical field
The present invention relates to spectrum complicated solution concentration analysis stoichiometry field, more particularly to a kind of multiposition modulation fluorescence
The method of free hemoglobin in excitation source measurement blood bag.
Background technology
In the prior art, more ripe technology is that free hemoglobin in blood bag is detected by way of chemical analysis
Content, there are the high outstanding advantages of accuracy, but the mode of chemical analysis needs opening blood bag taking-up sample to be chemically examined, can not
Meet quick, non-contact and free of contamination demand.
Found by the research to fluorescence spectrum, because its characteristic non-contact, pollution-free, with strong points is also possible to reality
The content detection of free hemoglobin in existing blood bag.
But blood scattering can cause the non-linear of spectrum, and be influenceed by spectral background noise, be asked for more than
Topic, method proposes the method for free hemoglobin in a kind of multiposition modulation fluorescence excitation light source measurement blood bag.
The content of the invention
The invention provides the method for free hemoglobin in a kind of multiposition modulation fluorescence excitation light source measurement blood bag, pole
The big non-linear effects for inhibiting blood scattered band, improve the precision of free hemoglobin content analysis in blood bag.Solve
The Non-Destructive Testing problem of free hemoglobin, efficient, pollution-free in blood bag, described below：
The method of free hemoglobin in a kind of multiposition modulation fluorescence excitation light source measurement blood bag, methods described include with
Lower step：
The light extraction optical port of fluorescence excitation light source is close to blood bag with the entrance slit of spectrum acquisition device, and modulating device modulation is glimmering
Phot-luminescence source makes its sender's wave optical signal, and fluorescent exciting source excitation free hemoglobin produces fluorescence, spectrum acquisition dress
Put collection fluorescence spectrum；
Displacement platform control fluorescence excitation light source is moved to multiple positions, and the glimmering of multiple positions is gathered by spectrum acquisition device
Light spectrum；
The fluorescence spectrum that multiple opening positions collect is transformed into frequency domain, constructed in frequency domain at fluorescence spectrum and normalization
Reason, the Comparative result with existing chemical analysis, founding mathematical models；
The fluorescence spectrum of blood sample in unknown blood bag in multiple opening positions is gathered, constructs fluorescence spectrum normalization in frequency domain
After bring mathematical modeling into, obtain the content of free hemoglobin；
Methods described increases the information content of free hemoglobin, suppresses the non-linear effects that blood scattered band comes；Eliminate light
Compose the influence of ambient noise；Improve the precision of free hemoglobin content analysis in blood bag.
It is specially the step of fluorescence spectrum in the construction frequency domain：
Modulation of source into square wave optical signal, is gathered fluorescence spectrum, by each wavelength by modulating device by spectrum acquisition device
Time series transform to frequency domain, with fluorescence spectrum in the fundametal compoment of each wavelength construction frequency domain.
Wherein, displacement platform control fluorescence excitation light source is moved to multiple positions, and multiple positions are gathered by spectrum acquisition device
The step of fluorescence spectrum put is specially：
Fluorescence excitation light source is excited at a of position to blood sample, and fluorescence spectrum is gathered by spectrum acquisition device；
Displacement platform control fluorescence excitation light source is moved to position b, and fluorescence spectrum is gathered by spectrum acquisition device；
Displacement platform control fluorescence excitation light source is moved to position n always, and fluorescence spectrum is gathered by spectrum acquisition device；
Or,
Blood sample in fluorescent exciting source excitation blood bag produces fluorescence, is gathered by spectrum acquisition device in position a
Fluorescence spectrum；
Displacement platform control spectrum acquisition device is moved to position b, gathers the fluorescence spectrum at the b of position；
Displacement platform control spectrum acquisition device is moved to position n, gathers the fluorescence spectrum at the n of position.
Wherein, methods described also includes：
One optical fiber is set at fluorescence excitation light source, as incident optical, and ensures incident optical and spectrum acquisition device
Entrance slit be close to blood bag；
Or,
One optical fiber is set at spectrum acquisition device, as the output optical fiber, and ensures the output optical fiber and fluorescence excitation light source
Light extraction optical port be close to blood bag；
Or,
Incident optical and the output optical fiber are set respectively at fluorescence excitation light source and spectrum acquisition device, and ensure incident light
Fibre is close to blood bag with the output optical fiber.
Wherein, a positions are the first position of incident optical, and fluorescence spectrum is gathered by spectrum acquisition device；Control into
Penetrate optical fiber to be moved at the b of position, fluorescence spectrum is gathered by spectrum acquisition device；Control incident optical is moved at the n of position always,
Fluorescence spectrum is gathered by spectrum acquisition device.
Wherein, a positions are the first position of the output optical fiber, and fluorescence spectrum is gathered by spectrum acquisition device；Control out
Penetrate optical fiber to be moved at the b of position, fluorescence spectrum is gathered by spectrum acquisition device；Control the output optical fiber is moved at the n of position always,
Fluorescence spectrum is gathered by spectrum acquisition device.
Further, the fluorescence excitation light source is ultraviolet lamp, can directly send ultraviolet light or be conducted through incident optical.
Further, institute's translation stage is stepper motor；The spectrum acquisition device is spectrometer；The modulating device
For chopper.
Further, the fluorescence excitation light source is ultraviolet laser tube or ultra-violet light-emitting pipe, can directly send ultraviolet light or
Person is conducted through incident optical.
Mathematical modeling described above utilizes principal component analysis, artificial neural network, PLS, supporting vector
Machine, signal analysis or statistical method are established.
The beneficial effect of technical scheme provided by the invention is：
1st, the fluorescence spectrum of the invention by gathering blood in the blood bag under same fluorescence excitation light source at diverse location,
The characteristic of fluorescence can be produced by ultraviolet excitation using free hemoglobin, makes measurement with strong points；
2nd, multiposition measuring has obtained the spectrum for exciting fluorescence to be scattered to multiple directions, is advantageous to reflect free hemoglobin
Scattering properties, add the information content of free hemoglobin, it is suppressed that the non-linear effects that blood scattered band comes；
3rd, construction frequency domain in fluorescence spectrum eliminate spectral background noise influence improve in blood bag dissociate blood red egg
The precision of white content analysis.Solve the problems, such as the Non-Destructive Testing of free hemoglobin in blood bag, it is efficient, pollution-free.
Brief description of the drawings
Fig. 1 is that multiposition modulates the schematic diagram that fluorescence excitation light source measures the method for free hemoglobin in blood bag；
Fig. 2 is that multiposition modulates the method signal that fluorescence excitation light source measures free hemoglobin in blood bag in embodiment 1
Figure；
Fig. 3 is that the method for free hemoglobin in multiposition modulation fluorescence excitation light source measurement blood bag in embodiment 2 is another
Schematic diagram；
Fig. 4 is that the method for free hemoglobin in multiposition modulation fluorescence excitation light source measurement blood bag in embodiment 3 is another
Schematic diagram；
Fig. 5 is that the method for free hemoglobin in multiposition modulation fluorescence excitation light source measurement blood bag in embodiment 4 is another
Schematic diagram；
Fig. 6 is that the method for free hemoglobin in multiposition modulation fluorescence excitation light source measurement blood bag in embodiment 5 is another
Schematic diagram；
Fig. 7 is that the method for free hemoglobin in multiposition modulation fluorescence excitation light source measurement blood bag in embodiment 6 is another
Schematic diagram.
In accompanying drawing, the list of parts representated by each label is as follows：
1：Fluorescence excitation light source； 2：Incident optical；
3：Blood bag； 4：Displacement platform；
5：Spectrum acquisition device； 6：The output optical fiber；
7：Modulating device； a：First position；
b：The second place； n：N-th position.
Embodiment
To make the object, technical solutions and advantages of the present invention clearer, embodiment of the present invention is made below further
It is described in detail on ground.
When modulating device is positioned among light source optical path, light can periodically by with block.Now light source is sent
Light is modulated into the square wave optical signal with certain frequency.Excite what the fluorescence frequency that free hemoglobin obtains was sent with light source
Square wave light frequency is consistent, by taking a certain wavelength X of caused fluorescence as an example, when Fig. 1 is that the waveform of λ wavelength integrates with spectrum acquisition device
Between corresponding integrated value schematic diagram, B is ambient noise, and T is the time of integration of spectrum acquisition device, and T1=T2=...=Ti=
T；T1It is ambient noise corresponding to the time of integration in section, the light intensity amplitude that now spectrum acquisition device receives is ambient noise
Integrated value, numerical value minimum is designated as Imin；TiIt is the light intensity and ambient noise of λ wavelength corresponding to the time of integration in section, this time
The light intensity amplitude that spectrum reception device receives is the light intensity of λ wavelength and the integrated value of ambient noise, and numerical value maximum is designated as Imax, T1
With TiBetween the corresponding ambient noises of other times of integration part, another part corresponds to the light intensity and ambient noise of λ wavelength, because
Integrated value obtained by this is in (Imin, Imax) change in section.Thus, in T1~TiThe interior class value domain that can obtain is (Imin,
Imax) integration value sequence, it can be seen that, the integrated value of the wavelength is in (Imin, Imax) change in section and form cyclical signal,
The integrated value of other wavelength is similar, and for strictly with cycle and synchronous cyclical signal.Pass through the product to each wavelength
The time series of score value carries out Fourier transform, fluorescence light in the frequency domain formed with the frequency domain fundametal compoment of all wavelengths integrated value
Spectrum, can eliminate spectral background noise, increase substantially signal to noise ratio.
Multiposition modulation fluorescence light source method eliminates the influence of spectral background noise, at the same using free hemoglobin by
Ultraviolet excitation can produce the characteristic of fluorescence, not only make measurement with strong points, and multiposition measuring has obtained exciting fluorescence to more
The spectrum of individual direction scattering, be advantageous to the scattering properties for reflecting free hemoglobin, add the information content of free hemoglobin,
The non-linear effects for inhibiting blood scattered band, improve the precision of free hemoglobin content analysis in blood bag.Solve
The Non-Destructive Testing problem of free hemoglobin, efficient, pollution-free in blood bag.
Embodiment 1
The method of free hemoglobin in multiposition modulation fluorescence excitation light source measurement blood bag provided in an embodiment of the present invention,
The device that uses as shown in Fig. 2 including：Fluorescence excitation light source 1, blood bag 3, displacement platform 4, spectrum acquisition device 5 and
Modulating device 7.
Wherein, ensure that the light extraction optical port of fluorescence excitation light source 1 is close to blood bag 3 with the entrance slit of spectrum acquisition device 5, adjust
Device 7 processed, which modulates fluorescence excitation light source 1, makes its sender's wave optical signal, and fluorescence excitation light source 1 is at a of first position to blood bag 3
Interior blood sample is excited, and fluorescence spectrum is gathered by spectrum acquisition device 5.Then fluorescence is controlled to swash by displacement platform 4
Light emitting source 1 is moved to second place b, gathers the fluorescence spectrum of the opening position, and fluorescence excitation light source 1 is controlled by displacement platform 4
Suppression is moved to the n-th position, gathers the fluorescence spectrum under the position.
The time series of each wavelength of the fluorescence spectrum of the how individual opening position collections of a, b ... n is transformed into frequency domain, with each
Normalized, method for normalizing are after fluorescence spectrum in the fundametal compoment construction frequency domain of wavelength：
Ag=A/max (A) (1)
In formula (1), AgTo normalize absorbance, max (A) is the absorbance maximum on different wave length, and A is extinction
Degree.With reference to the data of chemical analysis, principal component analysis (PCA, principal component analysis) or artificial is utilized
Neutral net (ANN, artificial neural network) or PLS (PLSR, particle least
Squares calibration analysis) or SVMs (SVM, support vector machines) signal point
The methods of analysis or statistics, can founding mathematical models.
The step of embodiment of the present invention is to specific founding mathematical models do not repeat, known to those skilled in the art.
The fluorescence spectrum for the multiple positions such as n that unknown blood sample gathered in a, b ..., construct in frequency domain normalizing after fluorescence spectrum
The above-mentioned mathematical modeling established is brought in change into, obtains the content of free hemoglobin.
To the model of each device in addition to specified otherwise is done, the model of other devices is not limited the embodiment of the present invention,
As long as the device of above-mentioned function can be completed.
In summary, the embodiments of the invention provide the blood that dissociates in a kind of multiposition modulation fluorescence excitation light source measurement blood bag
The method of Lactoferrin, not only eliminates the influence of spectral background noise, and adds the information content of free hemoglobin, it is suppressed that
The non-linear effects that blood scattered band comes, measurement is with strong points, improves the precision of free hemoglobin content analysis in blood bag.
Solve the problems, such as the Non-Destructive Testing of free hemoglobin in blood bag, it is efficient, pollution-free.
Embodiment 2
The embodiment of the present invention differs only in embodiment 1, fluorescence excitation light source 1, the movement with spectrum acquisition device 5
The difference of mode, it is described below：
Referring to Fig. 3, ensure that the light extraction optical port of fluorescence excitation light source 1 is close to blood bag with the entrance slit of spectrum acquisition device 5
3, modulating device 7, which modulates fluorescence excitation light source 1, makes its sender's wave optical signal, and fluorescence excitation light source 1 is to the blood sample in blood bag 3
Product are excited, and fluorescence spectrum is gathered in first position a by spectrum acquisition device 5.Spectrum is then controlled by displacement platform 4
Reception device 5 is moved to second place b, gathers the fluorescence spectrum at the b of the second place；Spectrum acquisition is controlled by displacement platform 4
Device 5 is moved to the n-th position always, gathers the fluorescence spectrum of the n-th opening position.
Wherein, fluorescence spectrum, normalization, founding mathematical models and the free blood red egg of calculating in follow-up construction frequency domain
The step of Bai Hanliang, is same as Example 1, and the embodiment of the present invention is not repeated this.
To the model of each device in addition to specified otherwise is done, the model of other devices is not limited the embodiment of the present invention,
As long as the device of above-mentioned function can be completed.
In summary, the embodiments of the invention provide the blood that dissociates in a kind of multiposition modulation fluorescence excitation light source measurement blood bag
The method of Lactoferrin, not only eliminates the influence of spectral background noise, and adds the information content of free hemoglobin, it is suppressed that
The non-linear effects that blood scattered band comes, measurement is with strong points, improves the precision of free hemoglobin content analysis in blood bag.
Solve the problems, such as the Non-Destructive Testing of free hemoglobin in blood bag, it is efficient, pollution-free.
Embodiment 3
During specific implementation, due to the limitation of space structure, it is possible that fluorescence excitation light source 1 and spectrum acquisition device 5
The situation of blood bag 3 can not be close to, at this moment can be by setting a light respectively at fluorescence excitation light source 1 and spectrum acquisition device 5
Fibre, as incident optical 2 and the output optical fiber 6.
Referring to Fig. 4, modulating device 7, which modulates fluorescence excitation light source 1, makes its sender's wave optical signal, and fluorescence excitation light source 1 leads to
Cross incident optical 2 to excite the blood sample in blood bag 3, fluorescence light is gathered by the output optical fiber 6 by spectrum acquisition device 5
Spectrum, incident optical 2 and the output optical fiber 6 are close to blood bag 3 respectively, and a positions are the first position of incident optical 2, spectrum acquisition device 5
Gather the fluorescence spectrum under the position；Then incident optical 2 is controlled to be moved at the b of the second place by displacement platform 4, spectrum connects
Receiving apparatus 5 gathers the fluorescence spectrum under the position；Incident optical 2 is controlled to be moved to the n-th opening position always by displacement platform 4,
Spectrum acquisition device 5 gathers the fluorescence spectrum under the position.
Wherein, fluorescence spectrum, normalization, founding mathematical models and the free blood red egg of calculating in follow-up construction frequency domain
The step of Bai Hanliang, is same as Example 1, and the embodiment of the present invention is not repeated this.
To the model of each device in addition to specified otherwise is done, the model of other devices is not limited the embodiment of the present invention,
As long as the device of above-mentioned function can be completed.
In summary, the embodiments of the invention provide the blood that dissociates in a kind of multiposition modulation fluorescence excitation light source measurement blood bag
The method of Lactoferrin, not only eliminates the influence of spectral background noise, and adds the information content of free hemoglobin, it is suppressed that
The non-linear effects that blood scattered band comes, measurement is with strong points, improves the precision of free hemoglobin content analysis in blood bag.
Solve the problems, such as the Non-Destructive Testing of free hemoglobin in blood bag, it is efficient, pollution-free.
Embodiment 4
The embodiment of the present invention is different only in that with embodiment 3, the output optical fiber 6, with first position a, second place b, n-th
The setting of position is different, described below：
Referring to Fig. 5, modulating device 7, which modulates fluorescence excitation light source 1, makes its sender's wave optical signal, and fluorescence excitation light source 1 leads to
Cross incident optical 2 to excite the blood sample in blood bag 3, fluorescence light is gathered by the output optical fiber 6 by spectrum acquisition device 5
Spectrum, incident optical 2 and the output optical fiber 6 are close to blood bag 3 respectively, and a positions are the first position of the output optical fiber 6, spectrum acquisition device 5
Gather the fluorescence spectrum under the position；Then the output optical fiber 6 is controlled to be moved at the b of the second place by displacement platform 4, spectrum connects
Receiving apparatus 5 gathers the fluorescence spectrum under the position；The output optical fiber 6 is controlled to be moved to the n-th opening position always by displacement platform 4,
Spectrum acquisition device 5 gathers the fluorescence spectrum under the position.
Wherein, fluorescence spectrum, normalization, founding mathematical models and the free blood red egg of calculating in follow-up construction frequency domain
The step of Bai Hanliang, is same as Example 1, and the embodiment of the present invention is not repeated this.
To the model of each device in addition to specified otherwise is done, the model of other devices is not limited the embodiment of the present invention,
As long as the device of above-mentioned function can be completed.
In summary, the embodiments of the invention provide the blood that dissociates in a kind of multiposition modulation fluorescence excitation light source measurement blood bag
The method of Lactoferrin, not only eliminates the influence of spectral background noise, and adds the information content of free hemoglobin, it is suppressed that
The non-linear effects that blood scattered band comes, measurement is with strong points, improves the precision of free hemoglobin content analysis in blood bag.
Solve the problems, such as the Non-Destructive Testing of free hemoglobin in blood bag, it is efficient, pollution-free.
Embodiment 5
As different from Example 3, the embodiment only includes incident optical 2 to the embodiment of the present invention, described below：
Referring to Fig. 6, modulating device 7, which modulates fluorescence excitation light source 1, makes its sender's wave optical signal, and fluorescence excitation light source 1 leads to
Cross incident optical 2 to excite the blood sample in blood bag 3, fluorescence spectrum, incident optical 2 are gathered by spectrum acquisition device 5
It is close to blood bag 3 respectively with the entrance slit of spectrum acquisition device 5, a positions are the first position of incident optical 2, spectrum acquisition device 5
Gather the fluorescence spectrum under the position；Then incident optical 2 is controlled to be moved at the b of the second place by displacement platform 4, spectrum connects
Receiving apparatus 5 gathers the fluorescence spectrum under the position；Incident optical 2 is controlled to be moved to the n-th opening position always by displacement platform 4,
Spectrum acquisition device 5 gathers the fluorescence spectrum under the position.
Wherein, fluorescence spectrum, normalization, founding mathematical models and the free blood red egg of calculating in follow-up construction frequency domain
The step of Bai Hanliang, is same as Example 1, and the embodiment of the present invention is not repeated this.
To the model of each device in addition to specified otherwise is done, the model of other devices is not limited the embodiment of the present invention,
As long as the device of above-mentioned function can be completed.
, can also be according to the needs in practical application, to first position a, the positions of second place b ... n-th during specific implementation
And mobile mode is set, i.e., can also include a variety of embodiments, the embodiment of the present invention is without limitation.
In summary, the embodiments of the invention provide the blood that dissociates in a kind of multiposition modulation fluorescence excitation light source measurement blood bag
The method of Lactoferrin, not only eliminates the influence of spectral background noise, and adds the information content of free hemoglobin, it is suppressed that
The non-linear effects that blood scattered band comes, measurement is with strong points, improves the precision of free hemoglobin content analysis in blood bag.
Solve the problems, such as the Non-Destructive Testing of free hemoglobin in blood bag, it is efficient, pollution-free.
Embodiment 6
As different from Example 3, the embodiment only includes the output optical fiber 6 to the embodiment of the present invention, described below：
Referring to Fig. 7, modulating device 7, which modulates fluorescence excitation light source 1, makes its sender's wave optical signal, and fluorescence excitation light source 1 is right
Blood sample in blood bag 3 is excited, and fluorescence spectrum, fluorescent exciting are gathered by the output optical fiber 6 by spectrum acquisition device 5
The light extraction optical port of source 1 and the output optical fiber 6 are close to blood bag 3 respectively, and a positions are the first position of fluorescence excitation light source 1, the output optical fiber 6
Gather the fluorescence spectrum under the position；Subsequent command displacement platform 4 controls fluorescence excitation light source 1 to be moved at the b of the second place, light
Compose the fluorescence spectrum under the collection of reception device 5 position；Command displacement platform 4 controls fluorescence excitation light source 1 to be moved to n-th always
Opening position, spectrum acquisition device 5 gather the fluorescence spectrum under the position.
Wherein, fluorescence spectrum, normalization, founding mathematical models and the free blood red egg of calculating in follow-up construction frequency domain
The step of Bai Hanliang, is same as Example 1, and the embodiment of the present invention is not repeated this.
, can also be according to the needs in practical application, to first position a, second place b and movement during specific implementation
Mode set, i.e., can also include a variety of embodiments.
To the model of each device in addition to specified otherwise is done, the model of other devices is not limited the embodiment of the present invention,
As long as the device of above-mentioned function can be completed.
In summary, the embodiments of the invention provide the blood that dissociates in a kind of multiposition modulation fluorescence excitation light source measurement blood bag
The method of Lactoferrin, not only eliminates the influence of spectral background noise, and adds the information content of free hemoglobin, it is suppressed that
The non-linear effects that blood scattered band comes, measurement is with strong points, improves the precision of free hemoglobin content analysis in blood bag.
Solve the problems, such as the Non-Destructive Testing of free hemoglobin in blood bag, it is efficient, pollution-free.
Embodiment 7
Select, the scheme in above-described embodiment 1-6 is further introduced, fluorescence swashs with reference to specific device
Light emitting source can be ultraviolet lamp, can directly send ultraviolet light or be conducted through incident optical 2, and displacement platform 4 is stepper motor, light
Spectrum reception device 5 is spectrometer, and modulating device 7 is chopper, described below：
As shown in figure 4, chopper 7, which modulates ultraviolet lamp 1, makes its sender's wave optical signal, ultraviolet lamp 1 passes through incident light
Blood sample in fine 2 pairs of blood bags 3 is excited, and fluorescence spectrum is gathered by the output optical fiber 6 by spectrometer 5, incident optical 2 with
The output optical fiber 6 is close to blood bag 3 respectively, and a positions are the first position of incident optical 2, and spectrometer 5 gathers the fluorescence light under the position
Spectrum；Then incident optical 2 is controlled to be moved at the b of the second place by stepper motor 4, spectrometer 5 gathers the fluorescence under the position
Spectrum；Incident optical 2 is controlled to be moved to the n-th opening position always by stepper motor 4, spectrometer 5 gathers the fluorescence under the position
Spectrum.
Wherein, fluorescence spectrum, normalization, founding mathematical models and the free blood red egg of calculating in follow-up construction frequency domain
The step of Bai Hanliang, is same as Example 1, and the embodiment of the present invention is not repeated this.
To the model of each device in addition to specified otherwise is done, the model of other devices is not limited the embodiment of the present invention,
As long as the device of above-mentioned function can be completed.
In summary, the embodiments of the invention provide the blood that dissociates in a kind of multiposition modulation fluorescence excitation light source measurement blood bag
The method of Lactoferrin, not only eliminates the influence of spectral background noise, and adds the information content of free hemoglobin, it is suppressed that
The non-linear effects that blood scattered band comes, measurement is with strong points, improves the precision of free hemoglobin content analysis in blood bag.
Solve the problems, such as the Non-Destructive Testing of free hemoglobin in blood bag, it is efficient, pollution-free.
Embodiment 8
As different from Example 7, fluorescence excitation light source 1 is ultraviolet laser tube to the embodiment of the present invention, can directly send purple
Outer light conducts through incident optical 2.
As shown in figure 4, chopper 7, which modulates ultraviolet laser tube 1, makes its sender's wave optical signal, ultraviolet laser tube 1 is by entering
Penetrate optical fiber 2 to excite the blood sample in blood bag 3, fluorescence spectrum, incident light are gathered by the output optical fiber 6 by spectrometer 5
Fibre 2 is close to blood bag 3 respectively with the output optical fiber 6, and a positions are the first position of incident optical 2, and spectrometer 5 is gathered under the position
Fluorescence spectrum；Then incident optical 2 is controlled to be moved at the b of the second place by stepper motor 4, spectrometer 5 is gathered under the position
Fluorescence spectrum；Incident optical 2 is controlled to be moved to the n-th opening position always by stepper motor 4, spectrometer 5 is gathered under the position
Fluorescence spectrum.
Wherein, fluorescence spectrum, normalization, founding mathematical models and the free blood red egg of calculating in follow-up construction frequency domain
The step of Bai Hanliang, is same as Example 1, and the embodiment of the present invention is not repeated this.
To the model of each device in addition to specified otherwise is done, the model of other devices is not limited the embodiment of the present invention,
As long as the device of above-mentioned function can be completed.
In summary, the embodiments of the invention provide the blood that dissociates in a kind of multiposition modulation fluorescence excitation light source measurement blood bag
The method of Lactoferrin, not only eliminates the influence of spectral background noise, and adds the information content of free hemoglobin, it is suppressed that
The non-linear effects that blood scattered band comes, measurement is with strong points, improves the precision of free hemoglobin content analysis in blood bag.
Solve the problems, such as the Non-Destructive Testing of free hemoglobin in blood bag, it is efficient, pollution-free.
Embodiment 9
For the embodiment of the present invention unlike embodiment 7,8, fluorescence excitation light source 1 is ultra-violet light-emitting pipe, can directly be sent
Ultraviolet light conducts through incident optical 2.
As shown in figure 4, chopper 7, which modulates ultra-violet light-emitting pipe 1, makes its sender's wave optical signal, ultra-violet light-emitting pipe 1 is by entering
Penetrate optical fiber 2 to excite the blood sample in blood bag 3, fluorescence spectrum, incident light are gathered by the output optical fiber 6 by spectrometer 5
Fibre 2 is close to blood bag 3 respectively with the output optical fiber 6, and a positions are the first position of incident optical 2, and spectrometer 5 is gathered under the position
Fluorescence spectrum；Then incident optical 2 is controlled to be moved at the b of the second place by stepper motor 4, spectrometer 5 is gathered under the position
Fluorescence spectrum；Incident optical 2 is controlled to be moved to the n-th opening position always by stepper motor 4, spectrometer 5 is gathered under the position
Fluorescence spectrum.
Wherein, fluorescence spectrum, normalization, founding mathematical models and the free blood red egg of calculating in follow-up construction frequency domain
The step of Bai Hanliang, is same as Example 1, and the embodiment of the present invention is not repeated this.
To the model of each device in addition to specified otherwise is done, the model of other devices is not limited the embodiment of the present invention,
As long as the device of above-mentioned function can be completed.
In summary, the embodiments of the invention provide the blood that dissociates in a kind of multiposition modulation fluorescence excitation light source measurement blood bag
The method of Lactoferrin, not only eliminates the influence of spectral background noise, and adds the information content of free hemoglobin, it is suppressed that
The non-linear effects that blood scattered band comes, measurement is with strong points, improves the precision of free hemoglobin content analysis in blood bag.
Solve the problems, such as the Non-Destructive Testing of free hemoglobin in blood bag, it is efficient, pollution-free.
Embodiment 10
The embodiment of the present invention is unlike above-described embodiment 7,8,9, need of the fluorescence excitation light source 1 in practical application
Fluorescence excitation light source, the displacement platform 4 of other models can also be used to use other mobile devices, spectrum acquisition
Device 5 can also use other reception devices.During specific implementation, the embodiment of the present invention is not limited the model of above-mentioned device
System.
The embodiment of the present invention is not restricted to position a, position b ... positions n and move mode etc., as long as this hair can be realized
The function of bright embodiment, within the protection domain of the application.
In summary, the embodiments of the invention provide the blood that dissociates in a kind of multiposition modulation fluorescence excitation light source measurement blood bag
The method of Lactoferrin, not only eliminates the influence of spectral background noise, and adds the information content of free hemoglobin, it is suppressed that
The non-linear effects that blood scattered band comes, measurement is with strong points, improves the precision of free hemoglobin content analysis in blood bag.
Solve the problems, such as the Non-Destructive Testing of free hemoglobin in blood bag, it is efficient, pollution-free.
Claims (10)
1. a kind of method of free hemoglobin in multiposition modulation fluorescence excitation light source measurement blood bag, it is characterised in that described
Method comprises the following steps：
The light extraction optical port of fluorescence excitation light source is close to blood bag with the entrance slit of spectrum acquisition device, and modulating device modulation fluorescence swashs
Light emitting source makes its sender's wave optical signal, and fluorescent exciting source excitation free hemoglobin produces fluorescence, and spectrum acquisition device is adopted
Collect fluorescence spectrum；
Displacement platform control fluorescence excitation light source is moved to multiple positions, and the fluorescence light of multiple positions is gathered by spectrum acquisition device
Spectrum；
The fluorescence spectrum that multiple opening positions collect is transformed into frequency domain, constructs fluorescence spectrum and normalized in frequency domain, with
The Comparative result of existing chemical analysis, founding mathematical models；
The fluorescence spectrum of blood sample in unknown blood bag in multiple opening positions is gathered, constructs in frequency domain band after fluorescence spectrum normalization
Enter mathematical modeling, obtain the content of free hemoglobin；
Methods described increases the information content of free hemoglobin, suppresses the non-linear effects that blood scattered band comes；Eliminate the spectrum back of the body
The influence of scape noise；Improve the precision of free hemoglobin content analysis in blood bag.
2. the side of free hemoglobin in a kind of multiposition modulation fluorescence excitation light source measurement blood bag according to claim 1
Method, it is characterised in that be specially the step of fluorescence spectrum in the construction frequency domain：
Modulation of source into square wave optical signal, fluorescence spectrum is gathered by spectrum acquisition device by modulating device, by each wavelength when
Between sequence transformation to frequency domain, with fluorescence spectrum in the fundametal compoment construction frequency domain of each wavelength.
3. the side of free hemoglobin in a kind of multiposition modulation fluorescence excitation light source measurement blood bag according to claim 1
Method, it is characterised in that displacement platform control fluorescence excitation light source is moved to multiple positions, and multiple positions are gathered by spectrum acquisition device
The step of fluorescence spectrum put is specially：
Fluorescence excitation light source is excited at a of position to blood sample, and fluorescence spectrum is gathered by spectrum acquisition device；
Displacement platform control fluorescence excitation light source is moved to position b, and fluorescence spectrum is gathered by spectrum acquisition device；
Displacement platform control fluorescence excitation light source is moved to position n always, and fluorescence spectrum is gathered by spectrum acquisition device；
Or,
Blood sample in fluorescent exciting source excitation blood bag produces fluorescence, and fluorescence is gathered in position a by spectrum acquisition device
Spectrum；
Displacement platform control spectrum acquisition device is moved to position b, gathers the fluorescence spectrum at the b of position；
Displacement platform control spectrum acquisition device is moved to position n, gathers the fluorescence spectrum at the n of position.
4. free hemoglobin in a kind of multiposition modulation fluorescence excitation light source measurement blood bag according to claim 1 or 2
Method, it is characterised in that methods described also includes：
One optical fiber is set at fluorescence excitation light source, as incident optical, and ensures that incident optical is incident with spectrum acquisition device
Slit is close to blood bag；
Or,
One optical fiber is set at spectrum acquisition device, as the output optical fiber, and ensures the output optical fiber and fluorescence excitation light source light extraction
Optical port is close to blood bag；
Or,
Incident optical and the output optical fiber are set respectively at fluorescence excitation light source and spectrum acquisition device, and ensure incident optical with
The output optical fiber is close to blood bag.
5. the side of free hemoglobin in a kind of multiposition modulation fluorescence excitation light source measurement blood bag according to claim 3
Method, it is characterised in that
A positions are the first position of incident optical, and fluorescence spectrum is gathered by spectrum acquisition device；Control incident optical moves into place
Put at b, fluorescence spectrum is gathered by spectrum acquisition device；Control incident optical is moved at the n of position always, by spectrum acquisition device
Gather fluorescence spectrum.
6. the side of free hemoglobin in a kind of multiposition modulation fluorescence excitation light source measurement blood bag according to claim 3
Method, it is characterised in that
A positions are the first position of the output optical fiber, and fluorescence spectrum is gathered by spectrum acquisition device；Control the output optical fiber moves into place
Put at b, fluorescence spectrum is gathered by spectrum acquisition device；Control the output optical fiber is moved at the n of position always, by spectrum acquisition device
Gather fluorescence spectrum.
7. the side of free hemoglobin in a kind of multiposition modulation fluorescence excitation light source measurement blood bag according to claim 3
Method, it is characterised in that the fluorescence excitation light source is ultraviolet lamp, can directly send ultraviolet light or be conducted through incident optical.
8. the side of free hemoglobin in a kind of multiposition modulation fluorescence excitation light source measurement blood bag according to claim 3
Method, it is characterised in that institute's translation stage is stepper motor；The spectrum acquisition device is spectrometer；The modulating device is
Chopper.
9. the side of free hemoglobin in a kind of multiposition modulation fluorescence excitation light source measurement blood bag according to claim 3
Method, it is characterised in that the fluorescence excitation light source is ultraviolet laser tube or ultra-violet light-emitting pipe, can directly send ultraviolet light or through entering
Penetrate fiber optic conduction.
A kind of 10. multiposition modulation fluorescence excitation light source measurement blood bag according to any claim in claim 1-3
The method of interior free hemoglobin, it is characterised in that
The mathematical modeling utilizes principal component analysis, artificial neural network, PLS, SVMs, signal point
Analysis or statistical method are established.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710529554.4A CN107421924A (en) | 2017-07-02 | 2017-07-02 | The method of free hemoglobin in multiposition modulation fluorescence excitation light source measurement blood bag |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710529554.4A CN107421924A (en) | 2017-07-02 | 2017-07-02 | The method of free hemoglobin in multiposition modulation fluorescence excitation light source measurement blood bag |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107421924A true CN107421924A (en) | 2017-12-01 |
Family
ID=60426783
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710529554.4A Pending CN107421924A (en) | 2017-07-02 | 2017-07-02 | The method of free hemoglobin in multiposition modulation fluorescence excitation light source measurement blood bag |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107421924A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7899506B2 (en) * | 2002-11-04 | 2011-03-01 | Tianjin Sunshine Optics Technolies Co. Ltd. | Composite spectral measurement method and its spectral detection instrument |
| CN106461539A (en) * | 2013-12-05 | 2017-02-22 | G·P·克林克翰莫 | Spectrophotometer with variable optical path length cell |
-
2017
- 2017-07-02 CN CN201710529554.4A patent/CN107421924A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7899506B2 (en) * | 2002-11-04 | 2011-03-01 | Tianjin Sunshine Optics Technolies Co. Ltd. | Composite spectral measurement method and its spectral detection instrument |
| CN106461539A (en) * | 2013-12-05 | 2017-02-22 | G·P·克林克翰莫 | Spectrophotometer with variable optical path length cell |
Non-Patent Citations (2)
| Title |
|---|
| 周梅: "《提高动态光谱信噪比的方法及应用》", 《中国博士学位论文全文数据库 医药卫生科技辑》 * |
| 李刚 等: "利用多光程光谱法检测备注多种成分含量的研究", 《光谱学与光谱分析》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101308090B (en) | Fire field multi- parameter laser wavelength modulated spectrum detection method and apparatus | |
| US8921277B2 (en) | Multiplexed flow assay based on absorption-encoded micro beads | |
| CN109477784A (en) | Fluorescence imaging flow cytometry with enhancing image resolution ratio | |
| CN104833650B (en) | The pulse terahertz time-domain spectroscopy system and detection method of monochromatic light lead antenna | |
| CN107367484A (en) | A kind of method of free hemoglobin content in nondestructive measurement blood bag | |
| CN106769971A (en) | A kind of infrared spectroscopy system based on femtosecond pump probe | |
| CN107044959A (en) | Micro- multi-modal fusion spectral detection system | |
| CN107991270A (en) | Blood species discrimination system based on coherent antistockes Raman spectroscopy | |
| CN205843813U (en) | A kind of device of all-fiber formula time domain short scan tera-hertz spectra based on fiber stretcher | |
| CN107356533A (en) | The method of free hemoglobin content in multiposition modulated light source measurement blood bag | |
| CN107655832B (en) | Based on the femtosecond Two-dimensional spectroscopy acquisition method converted in chirp and system | |
| CN107356575A (en) | The method that multiposition modulation fluorescence excitation light source measures packed complicated solution composition | |
| CN107421924A (en) | The method of free hemoglobin in multiposition modulation fluorescence excitation light source measurement blood bag | |
| CN108254336A (en) | A kind of terahertz light spectrometer | |
| CN205215187U (en) | Skin detecting system based on it is multispectral | |
| CN107290317A (en) | The method that double optical path modulation fluorescence excitation light sources measure packed complicated solution composition | |
| CN107356573A (en) | The method of free hemoglobin content in multiposition fluorescence spectral measuring blood bag | |
| CN107356574A (en) | The method of the packed complicated solution component content of multiposition fluorescence spectral measuring | |
| CN107421928A (en) | The method of free hemoglobin in double optical path modulation fluorescence excitation light source measurement blood bags | |
| CN107389634A (en) | Double light path and multiposition modulation fluorescence excitation light source measurement free hemoglobin methods | |
| CN107367493A (en) | The method that fluorescent intensity measures free hemoglobin in blood bag in double light path frequency domains | |
| CN107192699A (en) | The method that fluorescent intensity measures free hemoglobin in blood bag in multiposition frequency domain | |
| CN107421925A (en) | Multiposition modulation transmission and the method for fluorescence excitation light source measurement free hemoglobin | |
| CN107167440A (en) | The method of free hemoglobin content in double optical path modulation light source measurement blood bags | |
| CN107179307A (en) | The method of free hemoglobin content in double light path fluorescence spectral measuring blood bags |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20171201 |
|
| RJ01 | Rejection of invention patent application after publication |