CN107410801A - A kind of Animal Liver protolysate drink and preparation method thereof - Google Patents
A kind of Animal Liver protolysate drink and preparation method thereof Download PDFInfo
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- CN107410801A CN107410801A CN201710652234.8A CN201710652234A CN107410801A CN 107410801 A CN107410801 A CN 107410801A CN 201710652234 A CN201710652234 A CN 201710652234A CN 107410801 A CN107410801 A CN 107410801A
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- liver
- animal
- protolysate
- drink
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- 210000004185 liver Anatomy 0.000 title claims abstract description 77
- 241001465754 Metazoa Species 0.000 title claims abstract description 68
- 239000003531 protein hydrolysate Substances 0.000 title claims abstract description 32
- 238000002360 preparation method Methods 0.000 title claims abstract description 24
- 230000001954 sterilising effect Effects 0.000 claims abstract description 26
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 26
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 25
- 238000005238 degreasing Methods 0.000 claims abstract description 14
- 108010070915 orgotein Proteins 0.000 claims abstract description 14
- 229960004534 orgotein Drugs 0.000 claims abstract description 14
- TUGDLVFMIQZYPA-UHFFFAOYSA-N tetracopper;tetrazinc Chemical compound [Cu+2].[Cu+2].[Cu+2].[Cu+2].[Zn+2].[Zn+2].[Zn+2].[Zn+2] TUGDLVFMIQZYPA-UHFFFAOYSA-N 0.000 claims abstract description 14
- 239000012153 distilled water Substances 0.000 claims abstract description 13
- 235000003599 food sweetener Nutrition 0.000 claims abstract description 12
- 239000003765 sweetening agent Substances 0.000 claims abstract description 12
- 238000002604 ultrasonography Methods 0.000 claims abstract description 12
- 238000011049 filling Methods 0.000 claims abstract description 11
- 239000003381 stabilizer Substances 0.000 claims abstract description 11
- 239000001814 pectin Substances 0.000 claims abstract description 8
- 235000010987 pectin Nutrition 0.000 claims abstract description 8
- 229920001277 pectin Polymers 0.000 claims abstract description 8
- 238000004332 deodorization Methods 0.000 claims abstract description 6
- 238000004108 freeze drying Methods 0.000 claims abstract description 4
- 108010009736 Protein Hydrolysates Proteins 0.000 claims abstract description 3
- 239000007788 liquid Substances 0.000 claims description 26
- 108090000790 Enzymes Proteins 0.000 claims description 13
- 102000004190 Enzymes Human genes 0.000 claims description 13
- 229940088598 enzyme Drugs 0.000 claims description 13
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid group Chemical group C(CC(O)(C(=O)O)CC(=O)O)(=O)O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 12
- 230000009849 deactivation Effects 0.000 claims description 9
- 230000007062 hydrolysis Effects 0.000 claims description 9
- 238000006460 hydrolysis reaction Methods 0.000 claims description 9
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 claims description 8
- 239000000413 hydrolysate Substances 0.000 claims description 7
- 239000006228 supernatant Substances 0.000 claims description 7
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims description 6
- 239000008367 deionised water Substances 0.000 claims description 6
- 229910021641 deionized water Inorganic materials 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- 238000000265 homogenisation Methods 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 5
- 238000004321 preservation Methods 0.000 claims description 5
- 238000002203 pretreatment Methods 0.000 claims description 5
- 238000007789 sealing Methods 0.000 claims description 5
- 238000002791 soaking Methods 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 229930091371 Fructose Natural products 0.000 claims description 4
- 239000005715 Fructose Substances 0.000 claims description 4
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 claims description 4
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 claims description 4
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 claims description 4
- 239000000811 xylitol Substances 0.000 claims description 4
- 235000010447 xylitol Nutrition 0.000 claims description 4
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical group OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 claims description 4
- 229960002675 xylitol Drugs 0.000 claims description 4
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims description 3
- 235000015277 pork Nutrition 0.000 claims description 3
- 241000272525 Anas platyrhynchos Species 0.000 claims description 2
- 244000118681 Iresine herbstii Species 0.000 claims description 2
- 108010019160 Pancreatin Proteins 0.000 claims description 2
- 238000001816 cooling Methods 0.000 claims description 2
- 229940055695 pancreatin Drugs 0.000 claims description 2
- 229920001285 xanthan gum Polymers 0.000 claims description 2
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims 1
- 240000000111 Saccharum officinarum Species 0.000 claims 1
- 235000007201 Saccharum officinarum Nutrition 0.000 claims 1
- 239000003795 chemical substances by application Substances 0.000 claims 1
- 238000000502 dialysis Methods 0.000 claims 1
- 239000000661 sodium alginate Substances 0.000 claims 1
- 235000010413 sodium alginate Nutrition 0.000 claims 1
- 229940005550 sodium alginate Drugs 0.000 claims 1
- 239000000470 constituent Substances 0.000 abstract 1
- 235000008935 nutritious Nutrition 0.000 abstract 1
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 4
- 229930006000 Sucrose Natural products 0.000 description 4
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 4
- 230000008021 deposition Effects 0.000 description 4
- 235000018102 proteins Nutrition 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 239000005720 sucrose Substances 0.000 description 4
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 3
- OHDRQQURAXLVGJ-HLVWOLMTSA-N azane;(2e)-3-ethyl-2-[(e)-(3-ethyl-6-sulfo-1,3-benzothiazol-2-ylidene)hydrazinylidene]-1,3-benzothiazole-6-sulfonic acid Chemical compound [NH4+].[NH4+].S/1C2=CC(S([O-])(=O)=O)=CC=C2N(CC)C\1=N/N=C1/SC2=CC(S([O-])(=O)=O)=CC=C2N1CC OHDRQQURAXLVGJ-HLVWOLMTSA-N 0.000 description 3
- 235000009508 confectionery Nutrition 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- 210000000496 pancreas Anatomy 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 239000011669 selenium Substances 0.000 description 3
- 229910052711 selenium Inorganic materials 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
- 102000019197 Superoxide Dismutase Human genes 0.000 description 2
- 108010012715 Superoxide dismutase Proteins 0.000 description 2
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 235000021120 animal protein Nutrition 0.000 description 2
- 235000013361 beverage Nutrition 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- 229910052742 iron Inorganic materials 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 230000002906 microbiologic effect Effects 0.000 description 2
- 239000011707 mineral Substances 0.000 description 2
- 235000010755 mineral Nutrition 0.000 description 2
- 230000000050 nutritive effect Effects 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 150000003254 radicals Chemical class 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 229910052708 sodium Inorganic materials 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000011701 zinc Substances 0.000 description 2
- 229910052725 zinc Inorganic materials 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 235000007926 Craterellus fallax Nutrition 0.000 description 1
- XFXPMWWXUTWYJX-UHFFFAOYSA-N Cyanide Chemical compound N#[C-] XFXPMWWXUTWYJX-UHFFFAOYSA-N 0.000 description 1
- 240000007175 Datura inoxia Species 0.000 description 1
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 239000000783 alginic acid Substances 0.000 description 1
- 235000010443 alginic acid Nutrition 0.000 description 1
- 229960001126 alginic acid Drugs 0.000 description 1
- 229920000615 alginic acid Polymers 0.000 description 1
- 150000004781 alginic acids Chemical group 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 229910052785 arsenic Inorganic materials 0.000 description 1
- RQNWIZPPADIBDY-UHFFFAOYSA-N arsenic atom Chemical compound [As] RQNWIZPPADIBDY-UHFFFAOYSA-N 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 235000019197 fats Nutrition 0.000 description 1
- 238000007667 floating Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000003859 lipid peroxidation Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 description 1
- 230000017854 proteolysis Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/70—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
- A23L2/84—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter using microorganisms or biological material, e.g. enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Polymers & Plastics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Nutrition Science (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Zoology (AREA)
- Non-Alcoholic Beverages (AREA)
Abstract
The present invention relates to a kind of Animal Liver protolysate drink and preparation method thereof, wherein constituent content is calculated by weight as：1.5 ~ 2.5 parts of Animal Liver protolysate, 6 ~ 10 parts of sweetener, 0.08 ~ 0.12 part of pectin, 0.02 ~ 0.1 part of stabilizer, 87 ~ 92 parts of distilled water.Animal's liver is cleaned into deodorization and degreasing first during preparation, then using ultrasound-assisted enzymolysis, by dialysing, freeze-drying obtains orgotein hydrolysate, finally by allotment, homogeneous sterilization, it is filling after obtain Animal Liver protolysate drink.Preparation method of the present invention is simple, and step is easily operated, by the liver of animal be made drink it is in good taste, it is nutritious, conveniently drink, animal's liver has been obtained into new utilization, has realized bigger value.
Description
Technical field
The present invention relates to a kind of Animal Liver protolysate drink and preparation method thereof, belongs to animal protein beverage technology neck
Domain.
Background technology
Animal's liver is a kind of food of vegetal pole horn of plenty.In liver rich in protein, fat, carbohydrate, vitamin and
Mineral matter.Liver is not only a kind of high-quality complete protein source, and containing a variety of essential amino acids, and ratio is appropriate, simultaneously
Also containing substantial amounts of superoxide dismutase (SOD) etc., there is the effect of obvious lipid peroxidation inhibition, so as to protect cell and tissue not
It is damaged, has the function that to prevent tumour generation, anti-aging to a certain extent, improves immunity of organisms, there is very high answer
With value.Evaluated according to amino acid nutrient, the content highest of phenylalanine in animal's liver.In addition, animal's liver rich in zinc,
The mineral matter elements such as iron, selenium.According to nutritional quality index (INQ), wherein iron content highest.The elements such as zinc in addition, selenium contain
Amount is higher, and wherein selenium is important nutriment, being capable of the critical function such as prevention of cardiovascular.Although the nutritive value of liver compared with
Height, but up to the present, the deep processing extraction and its utilization research to animal's liver albumen are less.With animal's liver albumen
Enzymolysis product is primary raw material, adds a certain amount of stabilizer and sucrose and orgotein hydrolysis is made through techniques such as aquation, homogeneous, sterilizations
Thing beverage, the added value of low value animal's liver is on the one hand improved by deep processing；On the other hand, can also be by taking in nutritional ingredient
Abundant animal's liver enhancing human life power.However, animal's liver albumen emulsifies after being hydrolyzed to amino acid and small-molecular peptides
Property and emulsion stability be remarkably decreased so that product easily occurs protein in placement process and sunk and phenomena such as fat floating,
Product stability needs further to improve.
The content of the invention
The invention aims to solve the above problems, there is provided a kind of preparation method is simple, and step is easily operated
Animal Liver protolysate drink and preparation method thereof.
The present invention adopts the following technical scheme that：A kind of Animal Liver protolysate drink, each component content is in parts by weight
It is calculated as：1.5~2.5 parts of Animal Liver protolysate, 6~10 parts of sweetener, 0.08~0.12 part of pectin, stabilizer 0.02~
0.1 part, 87~92 parts of distilled water.
The preparation method of Animal Liver protolysate drink, comprises the following steps：
(1) pre-treatment：Animal's liver is cleaned and removes surface and internal film, is diced after soaking 2-3h deodorizations and powder
It is broken；10~20min of enzyme deactivation at 90~100 DEG C is placed in thermostat water bath, adds the different of animal's liver gross weight 8~10%
Propanol solution carries out degreasing, and degreasing time is 12~24h；
(2) ultrasonic wave added digests：The animal's liver of degreasing in step (1) is pressed into solid-liquid ratio 1:5~7 add distilled water, put
Ultrasound 3~7min of pretreatment is carried out in ultrasonic device, supersonic frequency is 100~200W, and ultrasound adds animal's liver after terminating
The pancreatin of weight 0.5~2.5%；Hydrolysis temperature is 20~50 DEG C, and pH is 7.5~8.0, and enzymolysis time is 90~150min；
(3) preparation of orgotein hydrolysate：Gone out being placed in step (2) by the feed liquid of enzymolysis in 90~100 DEG C of water-baths
10~20min of enzyme, 10~20min is centrifuged under 3000~10000g, take supernatant to dialyse in deionized water 48~72h, warp
Freeze-drying obtains orgotein hydrolysate；
(4) allocate：Meter takes 87~92 parts of distilled water in parts by weight, temperature is heated to as 80~85 DEG C, in stirring
Under be slowly added to 6~10 parts of sweetener, 0.08~0.12 part of pectin, then add 1.5~2.5 parts of Animal Liver protolysate,
0.02~0.1 part of stabilizer, addition acidity regulator to material liquid pH to 7.0~7.5 are fully added after dissolving；
(5) homogeneous is sterilized：By feed liquid by sterilization machine, in 83~87 DEG C of sterilizations, homogenization pressure is 20~30MPa；
(6) filling and two-stage sterilization：Feed liquid progress by homogeneous sterilization is filling, then quickly gone out in 90~95 DEG C
Bacterium, sealing preservation after cooling.
Animal Liver protolysate drink as claimed in claim 1, it is characterised in that：The stabilizer is alginic acid
Sodium, xanthans or the mixture of the two.
Further, the sweetener is xylitol, sucrose, fructose in proportion 1~3:1~2:1~3 mixes.
Further, the animal's liver is one or more of mixtures in pork liver, chicken gizzard, duck liver, foie gras.
Further, the acidity regulator is citric acid or phosphoric acid.
Further, the molecular cut off of the milipore filter used when being dialysed in the step (3)<300Da.
Further, the temperature of freeze-drying is -45 DEG C~-55 DEG C in the step (3), atmospheric pressure is 10~
100Pa。
Animal Liver protolysate drink in the present invention is under clean environment using pop can, vial, papery packaging
Any one airtight package in box.
Preparation method of the present invention is simple, and step is easily operated, will obtain orgotein hydrolysis after fresh animal's liver processing
Thing carries out the allotment of drink again, extends the holding time of animal's liver after treatment, saves the nutritional ingredient in liver,
Eliminate the fishy smell of fresh liver, it is formulated after drink taste and sweet mouthfeel, nutritive value is high, conveniently drinks, and animal's liver is obtained
New utilization have been arrived, have realized bigger value.
Embodiment
Below in conjunction with specific embodiment, the present invention is further illustrated.
Embodiment one：
The preparation method of Animal Liver protolysate drink, comprises the following steps：
(1) pre-treatment：Pork liver is cleaned and removes surface and internal film, dices and crushes after soaking 2h deodorizations；Juxtaposition
Enzyme deactivation 20min, the aqueous isopropanol for adding animal's liver gross weight 10% carry out degreasing, taken off at 90 DEG C in thermostat water bath
The fat time is 24h；
(2) ultrasonic wave added digests：The animal's liver of degreasing in step (1) is pressed into solid-liquid ratio 1:5 add distilled water, are placed in super
Ultrasound pretreatment 3min, supersonic frequency 200W are carried out in acoustic equipment, ultrasound adds the pancreas of animal's liver weight 0.5% after terminating
Enzyme；Hydrolysis temperature is 50 DEG C, pH 8.0, enzymolysis time 150min；
(3) preparation of orgotein hydrolysate：In step (2) enzyme deactivation in 90 DEG C of water-baths will be placed in by the feed liquid of enzymolysis
20min, 20min is centrifuged under 3000g, take supernatant to dialyse in deionized water 48h, it is freeze-dried to obtain orgotein hydrolysis
Thing；
(4) allocate：Meter takes 87 parts of distilled water in parts by weight, is heated to temperature as 85 DEG C, slowly adds under stirring
Enter 6 parts of sweetener, 0.08 part of pectin, then add 2.5 parts of Animal Liver protolysate, fully add stabilizer marine alga after dissolving
Sour 0.02 part of sodium, addition acidity regulator citric acid to material liquid pH to 7.0；Sweetener is xylitol, sucrose, fructose by 1:1:1
Mix；
(5) homogeneous is sterilized：By feed liquid by sterilization machine, in 83 DEG C of sterilizations, homogenization pressure 20MPa；
(6) filling and two-stage sterilization：Feed liquid progress by homogeneous sterilization is filling, it is cold then in 90 DEG C of quick sterilizations
But sealing preservation after.
Embodiment two：
The preparation method of Animal Liver protolysate drink, comprises the following steps：
(1) pre-treatment：Animal's liver is cleaned and removes surface and internal film, is diced after soaking 2.5h deodorizations and powder
It is broken；The enzyme deactivation 15min at 95 DEG C is placed in thermostat water bath, and the aqueous isopropanol for adding animal's liver gross weight 10% is carried out
Degreasing, degreasing time 20h；
(2) ultrasonic wave added digests：The animal's liver of degreasing in step (1) is pressed into solid-liquid ratio 1:6 add distilled water, are placed in super
Ultrasound pretreatment 5min, supersonic frequency 150W are carried out in acoustic equipment, ultrasound adds the pancreas of animal's liver weight 1.5% after terminating
Enzyme；Hydrolysis temperature is 30 DEG C, pH 8.0, enzymolysis time 100min；
(3) preparation of orgotein hydrolysate：In step (2) enzyme deactivation in 95 DEG C of water-baths will be placed in by the feed liquid of enzymolysis
15min, 15min is centrifuged under 5000g, take supernatant to dialyse in deionized water 60h, it is freeze-dried to obtain orgotein hydrolysis
Thing；
(4) allocate：Meter takes 90 parts of distilled water in parts by weight, is heated to temperature as 82 DEG C, slowly adds under stirring
Enter 8 parts of sweetener, 0.10 part of pectin, then add 2 parts of Animal Liver protolysate, fully add stabilizer 0.05 after dissolving
Part, acidity regulator phosphoric acid is added to material liquid pH to 7.2, and sweetener is xylitol, sucrose, fructose by 3:2:3 mix；
(5) homogeneous is sterilized：By feed liquid by sterilization machine, in 85 DEG C of sterilizations, homogenization pressure 25MPa；
(6) filling and two-stage sterilization：Feed liquid progress by homogeneous sterilization is filling, it is cold then in 92 DEG C of quick sterilizations
But sealing preservation after.
Embodiment three：
The preparation method of Animal Liver protolysate drink, comprises the following steps：
(1) pre-treatment：Animal's liver is cleaned and removes surface and internal film, dices and crushes after soaking 3h deodorizations；
The enzyme deactivation 10min at 100 DEG C is placed in thermostat water bath, and the aqueous isopropanol for adding animal's liver gross weight 10% is taken off
Fat, degreasing time 12h；
(2) ultrasonic wave added digests：The animal's liver of degreasing in step (1) is pressed into solid-liquid ratio 1:7 add distilled water, are placed in super
Ultrasound pretreatment 7min, supersonic frequency 100W are carried out in acoustic equipment, ultrasound adds the pancreas of animal's liver weight 2.5% after terminating
Enzyme；Hydrolysis temperature is 20 DEG C, pH 8.0, enzymolysis time 90min；
(3) preparation of orgotein hydrolysate：In step (2) enzyme deactivation in 100 DEG C of water-baths will be placed in by the feed liquid of enzymolysis
10min, 10min is centrifuged under 10000g, take supernatant to dialyse in deionized water 72h, it is freeze-dried to obtain orgotein water
Solve thing；
(4) allocate：Meter takes 92 parts of distilled water in parts by weight, is heated to temperature as 80 DEG C, slowly adds under stirring
Enter 10 parts of sweetener, 0.12 part of pectin, then add 1.5 parts of Animal Liver protolysate, fully add stabilizer 0.1 after dissolving
Part, addition acidity regulator citric acid to material liquid pH to 7.5；
(5) homogeneous is sterilized：By feed liquid by sterilization machine, in 87 DEG C of sterilizations, homogenization pressure 30MPa；
(6) filling and two-stage sterilization：Feed liquid progress by homogeneous sterilization is filling, it is cold then in 95 DEG C of quick sterilizations
But sealing preservation after.
The Animal Liver protolysate drink that Example two is prepared, the coefficient of stability (R) and rate of deposition are carried out respectively
(SR) measure.
The method and step of the coefficient of stability (R) is as follows：Three graduated centrifuge tubes are taken, are separately added into drink 50mL, with centrifugation point
Disembark with 3000r/min centrifuge 15min, take supernatant dilute 100 times after, with ultraviolet specrophotometer survey absorbance A 2, with from
The ratio of the absorbance A 1 of 100 times of dilution before the heart is coefficient of stability R, and calculation formula is：
Measurement result is as shown in table 1.
The assay method step of rate of deposition (SR) is as follows：Three portions of 10mL drinks are measured respectively to be placed in 15mL centrifuge tubes, in
40min is centrifuged with 3500rmp/min in centrifuge, outwells supernatant, centrifuge tube is then inverted 30min, draws and is remained on tube wall
Remaining liquid, it is accurate to claim precipitation quality, centrifugation rate is calculated, calculation formula is：
Measurement result is as shown in table 1.
Table 1
| Sample 1 | Sample 2 | Sample 3 | |
| The coefficient of stability (R) | 96% | 95% | 97% |
| Rate of deposition (SR) | 0.04% | 0.05% | 0.05% |
As shown in Table 1, the coefficient of stability for the Animal Liver protolysate drink being prepared using the present invention is more than 95%,
Rate of deposition is less than 0.05%, it was demonstrated that the drink property is stable, has good keeping qualities, drink of the invention is suitably stored in 2~30 DEG C
Cool environment under preserve.
The obtained Animal Liver proteolysis drink of the preparation of Example two is parallel to carry out ABTS three times+Free radical it is clear
Except measure.Deionized water is used as blank control group, VC solution is positive controls.As a result it is as shown in table 2.
Table 2
| Sample 1 | Sample 2 | Sample 3 | |
| ABTS+Clearance rate (%) | 92.7 | 93.2 | 93.5 |
As shown in Table 2, the Animal Liver protolysate drink being prepared using the present invention, which is had, preferably removes ABTS
+ free radical effect, there is preferable anti-oxidation function.
The drink in the embodiment of the present invention two is detected with reference to GB-16322-2003.
Organoleptic requirements：The present invention is in faint yellow, no fishy smell, and solution is uniformly without obvious sediment, sweet sweet tea.
Physical and chemical index：Total arsenic≤0.1 (mg/L)；Lead≤0.1 (mg/L)；Copper≤2.0 (mg/L)；Protein：8g/100mL；
It is not detected by cyanide.
Microbiological indicator：
Animal protein hydrolysate drink prepared by the present invention meets the requirement of physical and chemical index and microbiological indicator, has certain
Market prospects.
Claims (8)
- A kind of 1. Animal Liver protolysate drink, it is characterised in that：Each component content is calculated by weight as：Animal orgotein 1.5 ~ 2.5 parts of hydrolysate, 6 ~ 10 parts of sweetener, 0.08 ~ 0.12 part of pectin, 0.02 ~ 0.1 part of stabilizer, 87 ~ 92 parts of distilled water.
- 2. the preparation method of the Animal Liver protolysate drink described in claim 1, it is characterised in that：Comprise the following steps：（1）Pre-treatment：Animal's liver is cleaned and removes surface and internal film, dices and crushes after soaking 2 ~ 3h deodorizations；Put 10~20min of enzyme deactivation, the aqueous isopropanol for adding animal's liver gross weight 8 ~ 10% enter at 90 ~ 100 DEG C in thermostat water bath Row degreasing, degreasing time are 12 ~ 24h；（2）Ultrasonic wave added digests：By step（1）The animal's liver of middle degreasing presses solid-liquid ratio 1:5 ~ 7 add distilled water, are placed in ultrasound Ultrasound 3 ~ 7min of pretreatment is carried out in equipment, supersonic frequency be 100 ~ 200W, addition animal's liver weight 0.5 after ultrasound terminates ~ 2.5% pancreatin；Hydrolysis temperature is 20 ~ 50 DEG C, and pH is 7.5 ~ 8.0, and enzymolysis time is 90 ~ 150min；（3）The preparation of orgotein hydrolysate：By step（2）It is middle by enzymolysis feed liquid be placed in enzyme deactivation 10 in 90 ~ 100 DEG C of water-baths ~ 20min, 10 ~ 20min is centrifuged under 3000 ~ 10000g, take supernatant to dialyse in deionized water 48 ~ 72h, freeze-dried To orgotein hydrolysate；（4）Allotment：Meter takes 87 ~ 92 parts of distilled water in parts by weight, is heated to temperature as 80 ~ 85 DEG C, under stirring slowly 6 ~ 10 parts of sweetener is added, 0.08 ~ 0.12 part of pectin, then adds 1.5 ~ 2.5 parts of Animal Liver protolysate, fully dissolving 0.02 ~ 0.1 part of stabilizer, addition acidity regulator to material liquid pH to 7.0 ~ 7.5 are added afterwards；（5）Homogeneous is sterilized：By feed liquid by sterilization machine, in 83 ~ 87 DEG C of sterilizations, homogenization pressure is 20 ~ 30 MPa；（6）Filling and two-stage sterilization：Feed liquid progress by homogeneous sterilization is filling, then in 90 ~ 95 DEG C of quick sterilizations, cooling Sealing preservation afterwards.
- 3. Animal Liver protolysate drink as claimed in claim 1, it is characterised in that：The stabilizer be sodium alginate, Xanthans or the mixture of the two.
- 4. Animal Liver protolysate drink as claimed in claim 1, it is characterised in that：The sweetener is xylitol, sugarcane Sugar, fructose in proportion 1 ~ 3:1~2:1 ~ 3 mixes.
- 5. the preparation method of Animal Liver protolysate drink as claimed in claim 2, it is characterised in that：The animal's liver For one or more of mixtures in pork liver, chicken gizzard, duck liver, foie gras.
- 6. the preparation method of Animal Liver protolysate drink as claimed in claim 2, it is characterised in that：The acidity adjustment Agent is citric acid or phosphoric acid.
- 7. the preparation method of Animal Liver protolysate drink as claimed in claim 2, it is characterised in that：The step（3） The molecular cut off of the milipore filter used during middle dialysis<300Da.
- 8. the preparation method of Animal Liver protolysate drink as claimed in claim 2, it is characterised in that：The step（3） The temperature of middle freeze-drying is -45 DEG C ~ -55 DEG C, and atmospheric pressure is 10~100 Pa.
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