CN107397697B - Whitening and freckle removing composition and application thereof - Google Patents
Whitening and freckle removing composition and application thereof Download PDFInfo
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- CN107397697B CN107397697B CN201710763074.4A CN201710763074A CN107397697B CN 107397697 B CN107397697 B CN 107397697B CN 201710763074 A CN201710763074 A CN 201710763074A CN 107397697 B CN107397697 B CN 107397697B
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Abstract
The invention relates to the technical field of cosmetics, and discloses a whitening and freckle-removing composition which comprises the following components in parts by weight: 40-50 parts of collagen peptide, 5-10 parts of L-cysteine, 5-10 parts of glutathione, 5-10 parts of acerola cherry extract, 5-10 parts of citrus extract, 1-5 parts of black pepper extract and 1-10 parts of blueberry anthocyanin. The whitening and freckle-removing composition disclosed by the invention has the effect of whitening skin in all directions by resisting wrinkles, replenishing water, inhibiting tyrosinase activity, resisting oxidation, improving skin microcirculation and the like. Compared with a whitening product with a single action mechanism, the whitening product can remove melanin in cells to a greater extent and achieve a better whitening effect. Can be used for developing food, health product or medicine with whitening and speckle removing effects.
Description
Technical Field
The invention relates to a composition, in particular to a composition for whitening and removing freckles and application thereof.
Background
The whitening products on the market at present mainly reduce skin melanin from the following aspects. First, by inhibiting tyrosinase activity. Tyrosinase can catalyze tyrosine oxidation to generate dopa, dopaquinone and 5, 6-dihydroxyindole carboxylic acid, and further generate melanin. Inhibiting tyrosinase activity can inhibit melanin generation. Secondly, the destructive inhibition of the tyrosinase directly modifies and modifies the active site of the tyrosinase, so that the tyrosinase loses the catalytic oxidation effect on the tyrosine. Thirdly, the metabolism of melanin is accelerated. Reducing the melanin granules to white reducing melanin granules; or transferred to the dermis where it is catabolized by certain enzymes in the lysosomes of the cells of the dermis, or by the addition of enzymes that stimulate the catabolism of melanin in the cells, to shorten the metabolic cycle of melanin.
For example, the chinese invention with application number CN201010214873.4 "a whitening composition" contains cortex mori extract, licorice extract, morus alba extract, arbutin, kojic dipalmitate, niacinamide, tetrahydrocurcumin, humectant and preservative, and the effective whitening component of the invention inhibits the formation of melanin by inhibiting the activity of tyrosinase and blocking the transport of melanin.
Although the whitening products disclosed in the prior art can inhibit or destroy the activity of tyrosinase, accelerate the catabolism of melanin, reduce the source of the melanin to a certain extent and accelerate the route of the melanin. However, there is no clear solution for improving skin moisture, wrinkles and microcirculation, and the skin cannot be whitened in all directions, so that the effect is compromised.
Disclosure of Invention
In view of the above, the invention provides a whitening and freckle-removing composition, which is prepared by compounding various natural healthy raw materials, and achieves the effect of whitening skin through various action mechanisms such as wrinkle resistance, water supplement, tyrosinase activity inhibition, oxidation resistance, skin microcirculation improvement and the like.
In order to solve the technical problems, the technical scheme of the invention is as follows:
the invention provides a whitening and freckle-removing composition which comprises the following components in parts by weight: 40-50 parts of collagen peptide, 5-10 parts of L-cysteine, 5-10 parts of glutathione, 5-10 parts of acerola cherry extract, 5-10 parts of citrus extract, 1-5 parts of black pepper extract and 1-10 parts of blueberry anthocyanin.
Preferably, the whitening and freckle-removing composition comprises the following components in parts by weight: 43-48 parts of collagen peptide, 6-9 parts of L-cysteine, 6-9 parts of glutathione, 6-9 parts of acerola cherry extract, 6-9 parts of citrus extract, 2-4 parts of black pepper extract and 2-9 parts of blueberry anthocyanin.
More preferably, the whitening and freckle-removing composition comprises the following components in parts by weight: 44-46 parts of collagen peptide, 7-8 parts of L-cysteine, 7-8 parts of glutathione, 7-8 parts of acerola cherry extract, 7-8 parts of citrus extract, 3 parts of black pepper extract and 3-8 parts of blueberry anthocyanin.
The whitening and freckle-removing composition is prepared by compounding multiple natural healthy raw materials, and comprises the following components: the whitening and freckle-removing mask comprises collagen peptide, L-cysteine, glutathione, acerola cherry extract, black pepper extract, blueberry anthocyanin and citrus extract, wherein the components jointly achieve the effects of whitening and removing freckles through different action mechanisms. In the whitening and freckle-removing composition, the action mechanism of each component is as follows:
the collagen peptide can supplement high-quality amino acid raw materials of a human body, particularly proline and hydroxyproline, promote the synthesis of skin collagen, supplement collagen lost by the skin of an organism, lock water and store water, and delay skin aging. Meanwhile, superoxide anion and hydroxyl free radical can be removed to play an antioxidation role, and the formation of oxidation state melanin is reduced.
The L-cysteine can irreversibly and competitively inhibit the activity of tyrosinase diphenolase and block the formation of dopachrome. In addition, the L-cysteine has the function of dissolving cutin, can improve the property of the skin and enables the skin to become naturally whitened.
Glutathione is a free radical scavenger and antioxidant, and the active sulfhydryl group in the structure can reduce the bivalent copper in the tyrosinase structure into monovalent copper, so that the activity of catalyzing tyrosine to generate melanin is lost.
The acerola cherry extract and the citrus extract contain abundant vitamin C, which not only inhibits the activity of tyrosinase through antioxidation, but also can prevent the oxidation of other active ingredients in the composition.
The blueberry anthocyanin is used as an antioxidant enhancer of the composition, so that the antioxidant action time of the body is prolonged, and the whitening effect is deeper and more lasting.
The fructus Piperis extract has warm physical properties, and can promote blood flow, improve skin surface microcirculation, promote melanin metabolism, and prevent melanin aggregation, retention and dark spot formation.
The whitening and freckle-removing composition disclosed by the invention has the effect of whitening skin in all directions by resisting wrinkles, replenishing water, inhibiting tyrosinase activity, resisting oxidation, improving skin microcirculation and the like. Compared with a whitening product with a single action mechanism, the whitening and freckle removing composition disclosed by the invention can be used for removing melanin in cells to a greater extent, so that a better whitening effect is achieved.
The invention also provides application of the whitening and freckle-removing composition in food, health-care products or medicines, wherein the types of the food, the health-care products or the medicines comprise beverages, candies, powder, tablets, capsules, granules, oral liquid, drops, pills and the like. The product can achieve the effects of whitening and removing freckles.
Detailed Description
The invention provides a whitening and freckle-removing composition which is prepared by compounding multiple natural healthy raw materials. The components of the composition are as follows: the skin whitening cream comprises collagen peptide, L-cysteine, glutathione, acerola cherry extract, black pepper extract, blueberry anthocyanin and citrus extract, and achieves a better skin whitening effect through various action mechanisms of resisting wrinkles, supplementing water, inhibiting tyrosinase activity, resisting oxidation, improving skin microcirculation and the like.
In the whitening and freckle-removing composition, the addition amount of the collagen peptide is 40 to 50 parts by weight, preferably 43 to 48 parts by weight, and more preferably 44 to 46 parts by weight. The collagen peptide is a transparent light yellow collagen extract, has a molecular weight of 3000 daltons, is smaller than that of common collagen, and is easier to absorb. The specific structure of the collagen peptide can lock water strongly, and the collagen peptide is re-synthesized at the skin part as an amino acid raw material after being absorbed by the digestive tract of a human body, so that the water retention capacity of the skin can be increased, the water-oil balance of the skin can be kept, the water loss can be prevented, and the skin can be kept in a moist and tender state; the collagen peptide contains a large amount of hydroxyproline, can improve the skin brightness, has a special structure, can inhibit the activity of tyrosinase, reduces the formation of color spots and black spots, and has a whitening effect. The generation of wrinkles and fine wrinkles is related to the natural loss of collagen, and the collagen peptide is supplemented to be absorbed by a human body, and the fractured and aged elastic fiber net is repaired after the collagen peptide is synthesized on the skin part, so that the skin fiber organization mechanism is reformed, the elasticity is recovered, the flexibility is sufficient, and the fine wrinkles are relieved. In addition, the collagen can eliminate free radicals in vivo, resist oxidation in multiple ways, promote cell metabolism, accelerate melanin metabolism and delay cell aging. The collagen peptide has the triple effects of moisturizing, whitening and delaying aging. The raw material source of the collagen peptide is not particularly limited, and preferably, bones or skins of livestock or fish, such as pigskin, fish skin, fish bone, bovine bone, and the like, are used. The formulation of the collagen peptide product according to the present invention is not particularly limited. The invention has no special limit on the molecular weight of the collagen peptide, and the collagen peptide with smaller molecular weight is easier to be absorbed by the digestive tract, thereby achieving the effects of whitening, shrinking and resisting aging. Preferably the collagen peptides have a molecular weight of less than 3000 daltons, more preferably less than 2000 daltons, even more preferably less than 1000 daltons. In particular embodiments of the invention, commercially available collagen peptide products are preferably used. The brand name of the collagen peptide product is not particularly limited in the present invention, and in the specific examples of the present invention, the collagen peptide is purchased from rosinolo (guangdong) gelatin ltd, france.
In the whitening and freckle-removing composition, the addition amount of the L-cysteine is 5-10 parts by weight, preferably 6-9 parts by weight, and more preferably 7-8 parts by weight. The L-cysteine can regulate the bottom layer melanin capability generated by pigment cells at the lowest layer of the epidermis, and is an ideal natural whitening product. Meanwhile, the L-cysteine has the function of dissolving cutin, can change the property of the skin and enables the skin to become naturally whitened. In the present invention, L-cysteine also has the effects of ameliorating inflammatory and allergic skin conditions, and enhancing the skin's resistance and defense against external stimuli. The source of L-cysteine in the present invention is not particularly limited, and commercially available L-cysteine may be used. In a specific embodiment of the invention, L-cysteine was purchased from Wacker chemical (China) Inc.
In the whitening and freckle-removing composition, the addition amount of the glutathione is 5-10 parts by weight, preferably 6-9 parts by weight, and more preferably 7-8 parts by weight. Glutathione is a tripeptide containing gamma-amido bond and sulfhydryl group, and consists of glutamic acid, cysteine and glycine. In the invention, glutathione is used as a free radical scavenger and an antioxidant, and the active sulfydryl in the structure can reduce bivalent copper in the tyrosinase structure into monovalent copper, so that the monovalent copper loses the activity of catalyzing tyrosine to generate melanin, thereby reducing the sources of skin melanin and achieving the effects of whitening and removing freckles. The source of glutathione in the invention is not particularly limited, and commercial glutathione can be adopted. In a specific embodiment of the invention, glutathione is purchased from Langdian, Zhejiang.
In the whitening and freckle-removing composition, the acerola cherry extract is added in an amount of 5 to 10 parts by weight, preferably 6 to 9 parts by weight, and more preferably 7 to 8 parts by weight. Acerola is rich in vitamin C, and is the fruit which is known to be the most rich in vitamin C in the world at present. The VC content in 100 g of fruits reaches 2445 mg, which is far higher than 40mg of lemon, 68mg of citrus and 100mg of kiwi, while guava with extremely high vitamin C content is only 180mg all the time, which is a famous king of vitamin C. The acerola cherry extract is rich in VC and anti-aging factor (SOD), namely superoxide dismutase, can effectively remove free radicals generated in human activities, resist the invasion of external harmful substances, and has the effect of delaying aging. The source of acerola cherry extract in the present invention is not particularly limited, and commercially available acerola cherry extracts may be used. In a specific embodiment of the invention, acerola cherry extract is available from Shanghai Oufu Kogyo Co.
In the whitening and freckle-removing composition, the addition amount of the citrus extract is 5 to 10 parts by weight, preferably 6 to 9 parts by weight, and more preferably 7 to 8 parts by weight. The citrus of the present invention includes orange, grapefruit, lemon, mandarin orange, etc. The citrus extract of the present invention is preferably an extract of citrus pulp. The citrus extract is rich in VC, and has antioxidant effect. Meanwhile, the flavone component in the citrus extract can also promote the synthesis of skin collagen, and improve the moisturizing, elasticity and whitening effects of the skin. The source of the citrus extract is not particularly limited in the present invention, and commercially available citrus extracts can be used. In a specific embodiment of the invention, the citrus extract is purchased from Kyowa Biomedicine, Inc.
In the invention, the vitamin C contained in the acerola cherry extract and the citrus extract not only inhibits the activity of tyrosinase through antioxidation and reduces the formation of melanin, but also can prevent other active ingredients in the whitening and freckle-removing composition from being oxidized, thereby improving the stability of the effect of the composition.
In the whitening and freckle-removing composition, the addition amount of the blueberry anthocyanin is 1-10 parts by weight, preferably 2-9 parts by weight, and more preferably 3-8 parts by weight. The blueberry anthocyanidin is a derivative of flavone and flavanone. In the invention, the blueberry anthocyanin is used as an antioxidant enhancer of the composition, so that the antioxidant action time of the composition on organisms is prolonged, and the whitening effect is deeper and more lasting. The source of the blueberry anthocyanin is not specially limited, and the blueberry anthocyanin sold in the market is adopted. In a specific embodiment of the invention, the blueberry anthocyanins are purchased from Tianjin, Happy Natural products research and development Co.
In the whitening and freckle-removing composition, the addition amount of the black pepper extract is 1-5 parts by weight, preferably 2-4 parts by weight, and more preferably 3 parts by weight. In the invention, the main component of the black pepper extract is piperine, is warm and hot, can promote blood flow, improve skin surface microcirculation, promote melanin metabolism, prevent melanin aggregation, retention and dark spot formation, improve skin circulation on the whole, promote the absorption of other components on the skin surface and achieve the effects of whitening and removing spots. The source of the black pepper extract in the present invention is not particularly limited, and a commercially available black pepper extract may be used. In a specific embodiment of the invention, black pepper extract is purchased from Hippon Biomedicine, Inc.
The preparation method of the whitening and freckle-removing composition is not particularly limited, and the whitening and freckle-removing composition can be prepared by adopting a conventional preparation method in the field. In the specific embodiment of the invention, the whitening and freckle-removing composition is obtained by uniformly mixing the raw materials according to the proportion.
The whitening and freckle-removing composition can be added into food, health-care products or medicines to achieve the effects of whitening and removing freckles. The whitening and freckle-removing composition can be prepared into food, health care products or pharmaceutical products such as beverages, candies, powder, tablets, capsules, granules, oral liquid, drops, pills and the like. The addition amount of the whitening and freckle-removing composition in the product is preferably 1-50%, more preferably 10-40%, and even more preferably 20-35%.
As a preferable embodiment, the whitening and freckle-removing composition is prepared by taking 1-50 parts by weight of the whitening and freckle-removing composition, 40-50 parts by weight of microcrystalline cellulose, 10-30 parts by weight of maltodextrin, 0.5-3 parts by weight of hydroxypropyl methyl cellulose, 0.5-3 parts by weight of polyethylene glycol and 0.5-3 parts by weight of magnesium stearate, and is prepared by main processes of sieving, mixing, granulating, tabletting, coating, packaging and the like, and is applied to food, health care products or pharmaceutical products.
The present invention will be described in detail with reference to examples for better understanding the objects, technical solutions and advantages of the present invention, but they should not be construed as limiting the scope of the present invention.
Example 1
A whitening and freckle-removing composition comprises 50 parts by weight of collagen peptide, 5 parts by weight of L-cysteine, 5 parts by weight of glutathione, 5 parts by weight of acerola cherry extract, 5 parts by weight of citrus extract, 5 parts by weight of black pepper extract and 1 part by weight of blueberry anthocyanin.
The whitening and freckle-removing composition is obtained by mixing the raw materials in proportion.
Example 2
A whitening and freckle-removing composition comprises 45 parts by weight of collagen peptide, 8 parts by weight of L-cysteine, 7 parts by weight of glutathione, 7 parts by weight of acerola cherry extract, 8 parts by weight of citrus extract, 5 parts by weight of black pepper extract and 5 parts by weight of blueberry anthocyanin.
The whitening and freckle-removing composition is obtained by mixing the raw materials in proportion.
Example 3
A whitening and freckle-removing composition comprises 40 parts by weight of collagen peptide, 10 parts by weight of L-cysteine, 10 parts by weight of glutathione, 10 parts by weight of acerola cherry extract, 10 parts by weight of citrus extract, 5 parts by weight of black pepper extract and 10 parts by weight of blueberry anthocyanin.
The whitening and freckle-removing composition is obtained by mixing the raw materials in proportion.
Comparative example 1
The black pepper extracts of examples 1-3 were removed, and the other components and ratios were unchanged, and the obtained extracts were used as comparative examples 1-1, 1-2 and 1-3.
Comparative example 2
The glutathione in examples 1-3 was removed, and the other components and the ratio were unchanged, and the ratio was set as comparative example 2-1, comparative example 2-2 and comparative example 2-3, respectively.
Comparative example 3
The blueberry anthocyanidin in the examples 1-3 is removed, and other components and proportions are respectively used as a comparative example 3-1, a comparative example 3-2 and a comparative example 3-3 without changing.
Example 4
1. Measurement B16Cell culture and B16The cell proliferation activity, the specific experimental method and the result are as follows:
selection of B in logarithmic growth phase16Cells, adjusting the cell concentration to 2X 10 with the culture solution4Each per ml, 100ml of each well, inoculating the mixture into a 96-well plate, culturing for 12 hours, sucking supernatant after the cells adhere to the wall, respectively adding grape seeds (procyanidin) with different concentrations in example 2 and 200 mul of culture solution of 0.05 percent of vitamin E and vitamin C, simultaneously setting a blank control group, continuously culturing for 72 hours, measuring the light absorption value (A) of each well by adopting an MTT method, calculating the survival rate of the cells according to the following formula, and determining the safe concentration of the composition by taking the survival rate of the cells as a standard, wherein the survival rate of the cells is more than or equal to 90 percent.
Cell survival (%) ═ aTreatment of/AControl×100%
TABLE 1 compositions of example 1 vs. B16Effect of cell survival
Compared with the group with the concentration of 0.01%,*P<0.05,**p is less than 0.01; compared with grape seed (procyanidin), vitamin E and vitamin C,#P<0.05,##P<0.01。
the results show that the compositions of example 1 of the present invention are paired with B16Cell viability there was no significant difference between cell viability as mass concentration was increased. Example 1 therefore the same 0.05% concentration as grape seeds, vitamin E and vitamin C was selected for further experimental studies.
TABLE 2 compositions of example 2 vs. B16Effect of cell survival
Compared with the group with the concentration of 0.01%,*P<0.05,**p is less than 0.01; compared with grape seed (procyanidin), vitamin E and vitamin C,#P<0.05,##P<0.01。
the results show that the composition of example 2 of the present invention is paired with B16Cell viability there was no significant difference between cell viability as mass concentration was increased. Example 2 therefore the same 0.05% concentration as grape seeds, vitamin E and vitamin C was selected for further experimental studies.
TABLE 3 example 3 composition vs. B16Effect of cell survival
Compared with the group with the concentration of 0.01%,*P<0.05,**p is less than 0.01; compared with grape seed (procyanidin), vitamin E and vitamin C,#P<0.05,##P<0.01。
the results show that the composition of example 3 of the present invention is of pair B16Cell viability there was no significant difference between cell viability as mass concentration was increased. Example 3 therefore the same 0.05% concentration as grape seeds, vitamin E and vitamin C was selected for further experimental studies.
2. The content of melanin in cells is determined, and the specific experimental method and the result are as follows:
culture B16Adjusting the cell density to 4.5 × 10 from the logarithmic growth phase of the cells5Each/ml was inoculated into 6-well plates, 2ml per well, 5% CO at 37 ℃2Culturing in incubator for 12 hr, sucking out culture medium after cell adherence, adding 2ml of α -MSH (melanin stimulator) 100nM, adding 0.05% of the composition of examples 1-3, 0.05% of the composition of comparative example 1, grape seed (procyanidin) solution, vitamin E solution, and vitamin C solution into each well after 24 hr, setting up 3 multiple wells for each concentration, averaging, culturing for 72 hr, and adding 1000 μ l of 10% DMSO (dimethyl sulfoxide)Sulfoxide) was dissolved in NaOH solution (1M), heated at 90 ℃ for 2 hours, 100 μ l of cell lysate was aspirated, added to 96 wells, absorbance at 405nm was measured for each well, BCA method was used to measure protein content in each well and calculate relative melanin content in the cells, and each treatment factor was repeated 3 times.
Melanin synthesis relative content (%) - (value of absorbance of melanin content in administered group/absorbance of protein concentration in administered group)/(value of absorbance of melanin content in control group/absorbance of protein concentration in control group) x 100%
TABLE 4 Effect of the compositions of example 1 and comparative examples 1-1 on the melanin content of cells
Name of article | Example 1 | Comparative examples 1 to 1 | Grape seed | Vitamin E | Vitamin C |
Concentration of | 0.05% | 0.05% | 0.05% | 0.05% | 0.05% |
Relative content (%) | 71.3±3.3**# | 86.7±5.2 | 68.1±3.5 | 78.6±5.6 | 72.3±4.7 |
As shown in Table 4, the effect of the composition solution of example 1 on the melanin content in cells was significantly different from that of comparative example 1-1 at the same concentration of 0.05% ((**P is less than 0.01), and has significant difference compared with a vitamin E group (#P < 0.05) with no significant difference compared to the grape seed and vitamin C groups.
TABLE 5 Effect of the compositions of example 2 and comparative examples 1-2 on the melanin content of cells
Name of article | Example 2 | Comparative examples 1 to 2 | Grape seed | Vitamin E | Vitamin C |
Concentration of | 0.05% | 0.05% | 0.05% | 0.05% | 0.05% |
Relative content (%) | 67.3±3.1**## | 86.7±5.2 | 68.1±3.5 | 78.6±5.6 | 72.3±4.7 |
As shown in Table 5, the effect of the composition solution of example 2 on the melanin content of cells was significantly different from that of comparative examples 1 to 2 at the same concentration of 0.05% ((**P is less than 0.01), and has very significant difference compared with a vitamin E group (##P < 0.01) and no significant difference compared with the grape seed and vitamin C group.
TABLE 6 Effect of the compositions of example 3 and comparative examples 1-3 on the melanin content of cells
Name of article | Example 3 | Comparative examples 1 to 3 | Grape seed | Vitamin E | Vitamin C |
Concentration of | 0.05% | 0.05% | 0.05% | 0.05% | 0.05% |
Relative content (%) | 69.3±3.5**## | 86.7±5.2 | 68.1±3.5 | 78.6±5.6 | 72.3±4.7 |
As shown in Table 6, the effect of the composition solution of example 3 on the melanin content of cells was significantly different from that of comparative examples 1 to 3 at the same concentration of 0.05% ((**P is less than 0.01), and has significant difference compared with a vitamin E group (#P < 0.05) with no significant difference compared to the grape seed and vitamin C groups.
3. The specific experimental method and the results for measuring the activity of the tyrosinase of the cell are shown as follows:
culture B16Adjusting the cell density to 4.5 × 10 from the logarithmic growth phase of the cells5Each/ml was inoculated into 6-well plates, 2ml per well, 5% CO at 37 ℃2Culturing for 12 hours in an incubator, absorbing the culture solution after the cells are attached to the wall, adding 2ml of α -MSH (melanin stimulator) of 100nM, adding 0.05% of the composition of examples 1-3, 0.05% of the composition of comparative example 2, grape seed (procyanidin) solution, vitamin E solution and vitamin C solution into each well after 24 hours, setting up 3 multiple wells for averaging each concentration, culturing for 72 hours, adding 150 ul of 1% TritonX-100 (polyethylene glycol octylphenyl ether) into each tube after the cells are collected, dissolving for half an hour at 4 ℃, 13000r/min, centrifuging for 20min at 4 ℃, collecting the supernatant, adding 90 ul of cell lysate and 10 ul of 0.25% L-DOPA solution into each well of a 96-well plate, reacting for 60min at 37 ℃, measuring the absorbance of each well at 475nm by using a microplate reader, measuring the protein content of each well by a BCA method, calculating the relative activity of tyrosinase of the cells, and repeatedly measuring 3 times for each processing factor。
Tyrosinase relative activity (%) - ([ (absorbance value of administered group/absorbance value of administered group protein concentration)/(absorbance value of control group/absorbance value of control group protein concentration) ] × 100%
TABLE 7 Effect of the compositions of example 1 and comparative examples 2-1 on the tyrosinase activity of cells
Name of article | Example 1 | Comparative example 2-1 | Grape seed | Vitamin E | Vitamin C |
Concentration of | 0.05% | 0.05% | 0.05% | 0.05% | 0.05% |
Relative Activity (%) | 72.9±3.8**# | 88.5±3.9 | 72.1±2.8 | 78.6±5.1 | 80.3±4.3 |
As shown in Table 7, the effect of the composition solution of the present invention on tyrosinase activity was very significant compared to that of comparative example 2-1 at the same concentration of 0.05% ((**P is less than 0.01), and has significant difference compared with a vitamin C group (#P is less than 0.05), and has no significant difference compared with grape seeds and vitamin E groups.
TABLE 8 Effect of example 2 and comparative examples 2-2 compositions on cellular tyrosinase Activity
Name of article | Example 2 | Comparative examples 2 to 2 | Grape seed | Vitamin E | Vitamin C |
Concentration of | 0.05% | 0.05% | 0.05% | 0.05% | 0.05% |
Relative Activity (%) | 73.8±4.1**# | 88.5±3.9 | 72.1±2.8 | 78.6±5.1 | 80.3±4.3 |
As shown in Table 8, the effect of the composition solution of the present invention on tyrosinase activity was very significant compared to comparative examples 2-2 at the same concentration of 0.05% ((**P is less than 0.01), and has significant difference compared with a vitamin C group (#P is less than 0.05), and has no significant difference compared with grape seeds and vitamin E groups.
TABLE 9 Effect of example 3 and comparative examples 2-3 compositions on cellular tyrosinase Activity
Name of article | Example 3 | Comparative examples 2 to 3 | Grape seed | Vitamin E | Vitamin C |
Concentration of | 0.05% | 0.05% | 0.05% | 0.05% | 0.05% |
Relative Activity (%) | 73.3±2.9**# | 88.5±3.9 | 72.1±2.8 | 78.6±5.1 | 80.3±4.3 |
As shown in Table 9, the effect of the composition solution of the present invention on tyrosinase activity was very significant compared to comparative examples 2-3 at the same concentration of 0.05% ((**P is less than 0.01), and has significant difference compared with a vitamin C group (#P is less than 0.05), and has no significant difference compared with grape seeds and vitamin E groups.
4. The specific experimental method and results of the experiment for measuring and eliminating DPPH free radicals are as follows:
taking a certain amount of DPPH, and preparing a DPPH solution with the concentration of 0.08mmol/L by using absolute ethyl alcohol. 50 mu l of the composition of 0.05% of example 1 to 3, the composition of 0.05% of comparative example 3, a grape seed (procyanidin) solution, a vitamin E solution and a vitamin C solution are added into each detection hole of a 96-hole plate, 150 mu l of DPPH solution is added and uniformly mixed, the mixture is subjected to light-shielding reaction at room temperature for 30min, and the absorbance at 517nm is measured by an enzyme-linked immunosorbent assay. The DPPH free radical clearance rate is calculated according to the following formula, and collaborative analysis is carried out by adopting software.
DPPH radical clearance ═ ABlank group-ATreatment group)/ABlank group]×100%
TABLE 10 dosage effect of the ability of example 1 and comparative examples 3-1 to scavenge DPPH free radicals
The results show that example 1 shows a very significant difference in DPPH radical scavenging effect compared with the DPPH scavenging rate of comparative example 3-1 at the same concentration ((**P<0.01) Significant difference compared to vitamin E group: (#P is less than 0.05), and has no significant difference compared with vitamin C and grape seeds.
TABLE 11 dosage effect of the ability of example 2 and comparative examples 3-2 to scavenge DPPH free radicals
Name of article | Example 2 | Comparative examples 3 to 2 | Grape seed | Vitamin E | Vitamin C |
Concentration of | 0.05% | 0.05% | 0.05% | 0.05% | 0.05% |
Clearance (%) | 67.6±3.5**# | 52.8±4.1 | 68.1±3.8 | 59.6±4.7 | 67.6±4.1 |
The results show that2 the DPPH free radical scavenging effect is very different from the DPPH scavenging rate of the comparative example 3-2 with the same concentration ((**P is less than 0.01), and has significant difference compared with a vitamin E group (#P is less than 0.05), and has no significant difference compared with vitamin C and grape seeds.
TABLE 12 dosage effect table of DPPH radical scavenging ability of example 3 and comparative examples 3-3
Name of article | Example 3 | Comparative examples 3 to 3 | Grape seed | Vitamin E | Vitamin C |
Concentration of | 0.05% | 0.05% | 0.05% | 0.05% | 0.05% |
Clearance (%) | 69.5±3.1**# | 52.8±4.1 | 68.1±3.8 | 59.6±4.7 | 67.6±4.1 |
The results show that example 3 shows a very significant difference in DPPH radical scavenging effect compared to the DPPH scavenging rate of comparative examples 3-3 at the same concentration ((**P is less than 0.01), and has significant difference compared with a vitamin E group (#P is less than 0.05), and has no significant difference compared with vitamin C and grape seeds.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (5)
1. The whitening and freckle-removing composition is characterized by comprising the following components in parts by weight: 40-50 parts of collagen peptide, 5-10 parts of L-cysteine, 5-10 parts of glutathione, 5-10 parts of acerola cherry extract, 5-10 parts of citrus extract, 1-5 parts of black pepper extract and 1-10 parts of blueberry anthocyanin;
the molecular weight of the collagen peptide is less than 3000 daltons.
2. The whitening and freckle-removing composition according to claim 1, which is characterized by comprising the following components in parts by weight: 43-48 parts of collagen peptide, 6-9 parts of L-cysteine, 6-9 parts of glutathione, 6-9 parts of acerola cherry extract, 6-9 parts of citrus extract, 2-4 parts of black pepper extract and 2-9 parts of blueberry anthocyanin.
3. The whitening and freckle-removing composition according to claim 2, which is characterized by comprising the following components in parts by weight: 44-46 parts of collagen peptide, 7-8 parts of L-cysteine, 7-8 parts of glutathione, 7-8 parts of acerola cherry extract, 7-8 parts of citrus extract, 3 parts of black pepper extract and 3-8 parts of blueberry anthocyanin.
4. The whitening and freckle-removing composition of any one of claims 1 to 3 is applied to food, health products or medicines.
5. The use according to claim 4, wherein the types of food, health care or pharmaceutical products include beverages, candies, powders, tablets, capsules, granules, oral liquids, drops and pills.
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