CN107389935A - The detection method and viral antigen probe method for coating of a kind of viral antigen - Google Patents
The detection method and viral antigen probe method for coating of a kind of viral antigen Download PDFInfo
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- CN107389935A CN107389935A CN201710643983.4A CN201710643983A CN107389935A CN 107389935 A CN107389935 A CN 107389935A CN 201710643983 A CN201710643983 A CN 201710643983A CN 107389935 A CN107389935 A CN 107389935A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/02—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance
- G01N27/04—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating resistance
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
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Abstract
The present invention proposes a kind of method for detecting viral antigen, target inspection thing is placed in the reaction member for being coated with viral antigen probe, the viral antigen in thing is examined to detect the target in the reaction member by measuring unit, and detection information is stored and analyzed, obtain the testing result of viral antigen, testing result is easy to achieve, and is easy to those skilled in the art to analyze the testing result of storage, reduces testing cost；Further, viral antigen probe is coated on reaction member in advance, on the one hand reduces detection time, on the one hand the reaction member after screening coating carries out virus antigen detection, improves accuracy of detection and detection accuracy.
Description
Technical field
The present invention relates to bioscience research field, more particularly to a kind of detection method of viral antigen.
Background technology
Ribonucleic acid (Ribonucleic Acid, viral antigen) is present in biological cell and fractionated viral, viroid
In carrier of genetic information, viral antigen by ribonucleotide through phosphodiester bond condensation form long chain molecule.Detection virus
Antigen simultaneously extracts to detection data message, is a kind of strong tools of current research gene function, can be used for directly from source
Disease-causing gene " silence " is allowed on head, with treating cancer, or even AIDS, agriculturally will be also well worth doing.
Because target examines the influence of the concentration and surrounding enviroment of thing, existing virus antigen detection method, detection time
It is long, complex operation, and manual record result is needed, and higher is required to the professional technique of operating personnel, so as to cause detection speed
Degree is slow and testing cost is high.
Based on this, the present invention proposes a kind of virus antigen detection method, and simple to operate, accuracy of detection is higher, and passes through
Coating viral antigen probe in advance so that detection time is shorter, requires to reduce to the professional technique of operating personnel.
The content of the invention
It is an object of the invention to provide a kind of virus antigen detection method so that detection time is shorter, accuracy of detection compared with
Height, and it is simple to operate, relatively low is required to the professional technique of operating personnel.
In order to achieve the above object, the invention provides a kind of method for detecting viral antigen, examined for detecting target
Viral antigen in thing, it is characterised in that including：
Determine that the target examines thing can concentrations scope；
A reaction member is obtained, the reaction member is coated with viral antigen probe in advance；
Target inspection thing is placed in the reaction member, the viral antigen probe is examined in thing with the target
Viral antigen reacts；
The target is obtained by measuring unit and examines the data message of the viral antigen in thing, and is transmitted single to a storage
Member is stored；And
The data message in the memory cell is analyzed, obtains testing result.
Optionally, in the method for above-mentioned detection viral antigen, the reaction member includes a reaction chamber, and the virus is anti-
Former probe is coated on a detecting electrode of the bottom of the reaction chamber, and the detecting electrode is connected with the measuring unit.
Optionally, in the method for above-mentioned detection viral antigen, the reaction member is coated with viral antigen probe in advance
Step includes：
Obtain viral antigen probe solution corresponding with target inspection thing concentration；
The coating parameter of the reaction member is obtained, according to the coating parameter of the reaction member, it is anti-to obtain the virus
Coating time, temperature and the humidity of former probe solution；And
The viral antigen probe solution is placed in the reaction member, according to coating time, temperature and the humidity
The reaction member is coated with；Also include at least one layer of closure on the detecting electrode of the reaction member.
Optionally, wrapped in the method for above-mentioned detection viral antigen, the step of the coating parameter for obtaining the reaction member
Include：
First liquid is placed in the reaction member, first liquid includes deionized water, ultra-pure water, buffer solution, PBS
Any of solution, Buffer B and running water, the salt content of the ultra-pure water are less than 0.3mg/L, and electrical conductivity is less than 0.2
μs/cm；
Make the scanning of frequency electrical conductivity to the reaction member by 40Hz-4MHz of 50mV-10000mV sine voltage,
Recording responses electric current and phase difference simultaneously select several values in scanning result, the characteristic parameter CS as the reaction member0；
The first liquid is poured out, the viral antigen probe solution of various concentrations is placed in the reaction member, by 0-120 hours
At 20-30 degrees Celsius, relative humidity is to be placed in 70-90 space, and it is anti-that the viral antigen probe solution includes the virus
The solution of specific antibody caused by original, the specific antibody solution include neutralizing antibody, complement fixation antibody and blood clotting suppression
Antibody processed solution；
First liquid is placed in the reaction member, with 50mV-10000mV sine voltage by 40Hz-4MHz to anti-
Answer unit to do frequency impedance scanning, recording responses electric current and phase difference and select several values in scanning result, as described anti-
Answer the characteristic parameter CS of unit1；
The first liquid is poured out, confining liquid is loaded in the reaction member, by 0-120 hours at 20-30 degrees Celsius, relatively
Humidity is to be placed in 70-100 space；
First liquid is placed in the reaction member, with 50mV-10000mV sine voltage by 40Hz-4MHz to anti-
Answer unit to do frequency impedance scanning, recording responses electric current and phase difference and select several values in scanning result, as described anti-
Answer the characteristic parameter CS of unit2；And
The first liquid is poured out, examines thing to be put into the reaction member target for including viral antigen of various concentrations
It is interior, inspection is performed, under the conditions of various concentrations, obtains three characteristic parameter CS respectively0、CS1、CS2As described under respective concentration
The coating parameter of reaction member.
Optionally, it is described to pour out the first liquid in the method for above-mentioned detection viral antigen, the virus of various concentrations is resisted
Former probe solution is placed in the reaction member, and by 0-120 hours at 20-30 degrees Celsius, relative humidity is to be put in 70-90 space
Also include after the step of putting：
10Hz~10MHz excitation letter is applied to the reaction member of the viral antigen probe solution containing various concentrations
Number.
Optionally, in the method for above-mentioned detection viral antigen, the step of the coating parameter for obtaining the reaction member
Suddenly also include：
Obtaining characteristic parameter CS0Afterwards, cleaning liquid is placed in the reaction member to be detected, to described to be detected
Reaction member is cleaned, and the cleaning liquid includes any of deionized water, ultra-pure water, buffer solution and running water；
Obtaining characteristic parameter CS1Afterwards, cleaning liquid is placed in the reaction member to be detected, to described to be detected
Reaction member is cleaned；And
Obtaining characteristic parameter CS2Afterwards, cleaning liquid is placed in the reaction member to be detected, to described to be detected
Reaction member is cleaned.
Optionally, in the method for above-mentioned detection viral antigen, the method for the detection viral antigen also includes：
Examine thing to pre-process the target that can be in the range of concentrations, the pretreatment include filtering out it is described can
Target in the range of concentrations examines the particulate matter in thing.
Optionally, in the method for above-mentioned detection viral antigen, it is described determine that the target examines thing can concentrations
The step of scope, includes：
Choose concentration be ng/mL target examine thing, be diluted to 1ag/mL from ng/mL by exponential, using 10Hz~
10MHz pumping signal examines the viral antigen in thing to carry out full scan to the target, obtains the response of the viral antigen
Parameter and corresponding target examine the concentration range of thing, wherein, n >=0.5；
Response parameter and corresponding target to the viral antigen examine the relation of the concentration range of thing to be fitted, and obtain
Take fitting function；
According to the fitting function, obtain the target and examine the concentration range of thing, and/or obtained according to the fitting function
Take the response parameter of the viral antigen.
Optionally, in the method for above-mentioned detection viral antigen, the measuring unit work of the reaction member connection, obtain
The target examines the step of data message of the viral antigen in thing to include：
Apply 10Hz~10MHz pumping signal to reaction member by measuring unit, meanwhile, detect the resistance of reaction member
Anti- value changes, the impedance value of the reaction member is transmitted to a memory cell and stored.
Optionally, in the method for above-mentioned detection viral antigen, the data message in the analysis memory cell, obtain
The step of taking testing result includes：
Data processing, including denoising and amendment are carried out to the impedance value of the reaction member；
By the impedance value of the reaction member after data processing, testing result is obtained, and the testing result is shown in
One display unit.
In summary, the present invention proposes a kind of method for detecting viral antigen, by being coated with viral antigen probe in advance
Into a reaction member so that detection time is shorter, and accuracy of detection is higher, and the professional technique of operating personnel is required to reduce,
It is simple to operate；Further, target inspection thing is placed in the reaction member for being coated with viral antigen probe, passes through measuring unit
Examine the viral antigen in thing to detect the target in the reaction member, and detection information stored and analyzed,
The testing result of viral antigen is obtained, testing result is easy to achieve, and is easy to testing result of the those skilled in the art to storage
Analyzed, reduce testing cost.
Brief description of the drawings
Fig. 1 is the virus antigen detection method flow diagram in one embodiment of the present invention；
Fig. 2 is the flow chart for obtaining the method for the coating parameter of the viral antigen probe of embodiment in Fig. 1；
Fig. 3 is the measuring unit structural representation in one embodiment of the present invention.
Specifically, 1- measuring units；11- signaling modules；12 detection modules；2- memory cell；3- display units；4- reacts
Unit.
Embodiment
The embodiment of the present invention is described in more detail below in conjunction with schematic diagram.According to description below and
Claims, advantages and features of the invention will become apparent from.It should be noted that accompanying drawing is using very simplified form and
Using non-accurately ratio, only for the purpose of facilitating and clarifying the purpose of the embodiments of the invention.
The present invention proposes a kind of virus antigen detection method, for detecting the viral antigen in target inspection thing, operation letter
Single, detection time is shorter, and accuracy of detection is higher, and the professional technique of operating personnel is required to reduce.Specifically, with reference to figure 1 and
Fig. 3, the virus antigen detection method in one embodiment of the present invention include：
Step S1：Determine that the target examines thing can concentrations scope.
In a preferred embodiment, choose the target that concentration is 10g/mL and examine thing, it is diluted from 10g/mL
To 1ag/mL, full scan is carried out to viral antigen using 10Hz~10MHz pumping signal, chooses the response of the viral antigen
Parameter, it is determined that corresponding target examines the concentration range of thing；
Response parameter and corresponding target to the viral antigen examine the relation of the concentration range of thing to be fitted, and obtain
Take fitting function；
According to the fitting function, obtain the target and examine the concentration range of thing, and/or obtained according to the fitting function
Take the response parameter of the viral antigen..
Optionally, dilute the target that the concentration is 10g/mL examine thing can be, but not limited to include by it is exponential by its from
10g/mL is diluted to 1ag/mL, i.e. concentration after dilution next time is 1/10th of the concentration after this dilution.The present invention is to dilute
Releasing the target that the concentration is 10g/mL examines method and multiple of thing etc. to be not intended to be limited in any.
Step S2：A reaction member 4 is obtained, obtains a reaction member 4, it is anti-that the reaction member 4 is coated with virus in advance
Former probe, for detecting the viral antigen in the target inspection thing.
Specifically, the reaction member 4 includes a reaction chamber, the viral antigen probe is coated in the bottom of the reaction chamber
On one detecting electrode in portion, the detecting electrode is connected with a measuring unit 1.Specifically, the reaction member is coated with virus
Antigen probe, and the viral antigen probe can examine the viral antigen in thing that immune response, the disease occurs with the target
It is specific antibody caused by the viral antigen that malicious antigen probe, which includes but is not limited to, and the specific antibody includes but unlimited
One kind or any combination in neutralizing antibody, complement fixation antibody and hemagglutination inhibition antibody, the present invention do not do any limit to this
System.
Optionally, it is measles virus that the viral antigen, which includes but is not limited to, poliovirus, Coxsackie virus,
Epidemic encephalitis B virus, dengue virus, hemorrhagic fever viruse, Hantaan virus, Ebola virus, hydrophobin, the mankind exempt from
Epidemic disease defective virus, HTLV, hepatitis A virus, hepatitis type B virus, HCV, hepatitis D disease
Poison, HEV, herpes simplex virus, varicella virus, cytomegalovirus, EBV, human papilloma virus,
One kind or any combination in rotavirus, coronavirus and rubella virus.Optionally, if the viral antigen include it is angstrom rich
Virus and two kinds of antigens of hydrophobin are drawn, then the viral antigen probe then includes Ebola virus antibody and hydrophobin
Two kinds of antibody, the present invention do not do any restrictions to this.
Optionally, at least one layer of sealer is also included on the detecting electrode of the reaction member 4.The sealer is typically adopted
Prepared with unrelated protein, prevent other materials to be adsorbed onto on detecting electrode and testing result is impacted, one improves the standard of detection
True property.
Include specifically, the reaction member 4 is coated with the step of viral antigen probe in advance：
The coating parameter of the reaction member 4 is obtained, according to the coating parameter of the reaction member 4, obtains the virus
Coating time, temperature and the humidity of antigen probe solution；
The viral antigen probe solution is placed in the reaction member 4, according to coating time, temperature and the humidity
The reaction member 4 is coated with.
Viral antigen probe in the present embodiment is coated on the viral antigen probe in advance, on the one hand reduces detection
Time, on the one hand the reaction member progress virus antigen detection after screening coating, improves accuracy of detection and detection accuracy, right
The professional technique requirement of detection operating personnel also decreases.
Step S3：Target inspection thing is placed in the reaction member 4 for being coated with viral antigen probe, the virus
Antigen probe examines the viral antigen of thing that immune response occurs with the target.
Step S4：The measuring unit 1 being connected with the reaction member 4 works, and obtains the virus in the target inspection thing
The data message of antigen, and transmit to a memory cell 2 and stored.
Specifically, pumping signal is applied to reaction member 4 by the signaling module 11 in measuring unit 1, meanwhile, detect mould
Block 12 detects the data message of viral antigen, and the data information transfer of the viral antigen a to memory cell 2 is stored.
Optionally, the data message of the viral antigen in the present invention includes but is not limited to the impedance value of the reaction member
The impedance value of the reaction member is transmitted to a memory cell 2 and stored by change, detection module 12.Virus in step S3
Antigenic compound adds the thickness of the detecting electrode of the reaction member, and therefore, the impedance value of reaction member can change,
Preferably, in one embodiment of the invention, detect the change in impedance value of reaction member, by the impedance value of the reaction member transmit to
One memory cell is stored.
S5：The data message in the memory cell 2 is analyzed, obtains testing result.
Specifically, those skilled in the art can analyze and obtain according to the change in impedance value of the reaction member 4 in the present embodiment
Testing result, viral antigen species that the testing result includes but is not limited to examine for the target in thing, quantity and normal
Situation etc.；The Antibody-antigen complex that those skilled in the art can be generated by extracting immune response, obtains the target and examines
Composition and quantity of viral antigen in thing etc., are further studied.
Optionally, data processing, including denoising and amendment are carried out to the impedance value of the reaction member 4, passes through number
According to the impedance value of the reaction member after processing, testing result is obtained, is shown in a display unit 3.Optionally, the detection is tied
Fruit shows that it is PC or smart mobile phone that the display unit 3, which includes but is not limited to, in order to technical staff by a display unit 3
Inquired about and analyzed, the present invention is not intended to be limited in any to this.
Optionally, in addition to step S6：Thing is examined to pre-process the target that can be in the range of concentrations, it is described
Pretreatment includes filtering out the target that can be in the range of concentrations and examines particulate matter in thing, to increase detection accuracy.
In order to increase virus antigen detection precision, the target screened in further embodiment of this invention suitable for certain concentration is examined
The step reference chart 2 of the coating viral antigen probe of thing, specifically, the step of obtaining the coating parameter of the reaction member is wrapped
Include：
Step Sa：First liquid is placed in reaction member 4, first liquid includes deionized water, ultra-pure water, buffering
Liquid, PBS solution, Buffer B and running water or any combination, 40Hz-4MHz is pressed with 50mV-10000mV sine voltage
The scanning of frequency electrical conductivity, recording responses electric current and phase difference are done to each reaction member and selects several in scanning result
Value, the characteristic parameter CS as the reaction member0；
Step Sb：The first liquid is poured out, the viral antigen probe solution of various concentrations is placed in inside reaction member 4, pressed
0-120 hours, relative humidity was to be placed in 70-90 space at 20-30 degrees Celsius.
Optionally, precision is screened to realize, at 0 hour, 20 degrees Celsius, the space that relative humidity is 70, which is placed, was,
20 degrees Celsius, the space that relative humidity is 70 by the viral antigen probe solution of the various concentrations as the moment of reaction member 4,
The invention is not limited in this regard.
The specific antibody can examine the viral antigen in thing that immune response occurs with the target, so as to change
The impedance value of reaction member 4 is stated, in one embodiment of the invention, by the change of the impedance value of the reaction member to be detected to institute
Viral antigen is stated to be detected；Optionally, those skilled in the art can be compound by extracting the antibody-antigene of immune response generation
Thing, obtain the target and examine composition and quantity of viral antigen in thing etc., further studied.
Step Sc：First liquid is placed in each reaction member, and it is molten that first liquid includes deionized water, ultra-pure water, PBS
Any of liquid and Buffer B, make frequency to reaction member by 40Hz-4MHz of 50mV-10000mV sine voltage
Rate impedance scan, recording responses electric current and phase difference simultaneously select several values of scanning result, the feature as the reaction member
Parameter CS1；
Step Sd：The first liquid is poured out, confining liquid is loaded in reaction member, by 0-120 hours at 20-30 degrees Celsius,
Relative humidity is to be placed in 70-100 space；
Step Se：First liquid is placed in each reaction member, 40Hz- is pressed with 50mV-10000mV sine voltage
4MHz does frequency impedance scanning, recording responses electric current and phase difference to reaction member and selects several values of scanning result, makees
For the characteristic parameter CS of the reaction member2；
Step Sf：The first liquid is poured out, examines thing to be put into anti-the target of the known various concentrations for including viral antigen
Answer in unit, perform inspection, under the conditions of various concentrations, obtain three coating parameter CS respectively0、CS1、CS2, as corresponding dense
The coating parameter of the lower viral antigen probe solution of degree.Reaction member 4 includes corresponding viral antigen probe, it is determined that reaction
The coating parameter of unit 4, the target as selected suitable for certain concentration examine the viral antigen probe of thing.
Optionally, the step of coating parameter of the acquisition reaction member also includes：
Obtaining characteristic parameter CS0Afterwards, cleaning liquid is placed in the reaction member to be detected, to described to be detected
Reaction member is cleaned, and the cleaning liquid includes any of deionized water, ultra-pure water, buffer solution and running water；
Obtaining characteristic parameter CS1Afterwards, cleaning liquid is placed in the reaction member to be detected, to described to be detected
Reaction member is cleaned；And
Obtaining characteristic parameter CS2Afterwards, cleaning liquid is placed in the reaction member to be detected, to described to be detected
Reaction member is cleaned.
Cleaning to reaction member can reduce the other impurities in reaction member, and then add the detection of the reaction member
Precision.
The target for being applied to certain concentration by filtering out examines the viral antigen probe of thing, and the target of certain concentration is examined
The viral antigen tested in thing is detected, and is on the one hand coated with viral antigen probe in advance, is reduced detection time, on the other hand,
The reaction member selected by step Sa- steps Sf, can obtain corresponding standing time, temperature and humidity, increase detection
Accuracy, meanwhile, testing process is simple, and the professional technique of operating personnel is required to reduce, so as to reduce testing cost, had
Feasibility.
Optionally, in addition to step Sg：The reaction member 4 of the viral antigen probe solution containing various concentrations is applied
Add 10Hz~10MHz pumping signal, be easy to be internally formed partial fluid caused by significant thermograde in reaction member
Flowing, coating speed of the increase viral antigen probe solution on detecting electrode, the present invention do not do any restrictions to this.
In summary, the present invention proposes a kind of method for detecting viral antigen, by being coated with viral antigen probe in advance
Into a reaction member so that detection time is shorter, and accuracy of detection is higher, and the professional technique of operating personnel is required to reduce,
It is simple to operate；Further, target inspection thing is placed in the reaction member for being coated with viral antigen probe, passes through measuring unit
Examine the viral antigen in thing to detect the target in the reaction member, and detection information stored and analyzed,
The testing result of viral antigen is obtained, testing result is easy to achieve, and is easy to testing result of the those skilled in the art to storage
Analyzed, reduce testing cost.
The preferred embodiments of the present invention are above are only, any restrictions effect is not played to the present invention.Belonging to any
Those skilled in the art, in the range of technical scheme is not departed from, to the invention discloses technical scheme and
Technology contents make the variation such as any type of equivalent substitution or modification, belong to the content without departing from technical scheme, still
Belong within protection scope of the present invention.
Claims (10)
- A kind of 1. method for detecting viral antigen, for detecting the viral antigen in target inspection thing, it is characterised in that including：Determine that the target examines thing can concentrations scope；A reaction member is obtained, the reaction member is coated with viral antigen probe in advance；Target inspection thing is placed in the reaction member, the viral antigen probe examines the virus in thing with the target Antigen reacts；The target is obtained by measuring unit and examines the data message of the viral antigen in thing, and transmits to a memory cell Row storage；AndThe data message in the memory cell is analyzed, obtains testing result.
- 2. the method for detection viral antigen as claimed in claim 1, it is characterised in that the reaction member includes a reaction Chamber, the viral antigen probe are coated on a detecting electrode of the bottom of the reaction chamber, the detecting electrode and the survey Measure unit connection.
- 3. the method for detection viral antigen as claimed in claim 2, it is characterised in that the reaction member is coated with virus in advance The step of antigen probe, includes：Obtain viral antigen probe solution corresponding with target inspection thing concentration；The coating parameter of the reaction member is obtained, according to the coating parameter of the reaction member, the viral antigen is obtained and visits Coating time, temperature and the humidity of pin solution；AndThe viral antigen probe solution is placed in the reaction member, according to coating time, temperature and the humidity to institute Reaction member is stated to be coated with；Also include at least one layer of closure on the detecting electrode of the reaction member.
- 4. the method for detection viral antigen as claimed in claim 3, it is characterised in that obtain the coating ginseng of the reaction member Several steps include：First liquid is placed in the reaction member, it is molten that first liquid includes deionized water, ultra-pure water, buffer solution, PBS Any of liquid, Buffer B and running water, the salt content of the ultra-pure water are less than 0.3mg/L, and electrical conductivity is less than 0.2 μ s/cm；Make the scanning of frequency electrical conductivity, record to the reaction member by 40Hz-4MHz of 50mV-10000mV sine voltage Response current and phase difference simultaneously select several values in scanning result, the characteristic parameter CS as the reaction member0；The first liquid is poured out, the viral antigen probe solution of various concentrations is placed in the reaction member, existed by 0-120 hours 20-30 degrees Celsius, relative humidity is to be placed in 70-90 space, and the viral antigen probe solution includes the viral antigen The solution of caused specific antibody, the specific antibody solution include neutralizing antibody, complement fixation antibody and blood clotting and suppressed Antibody-solutions；First liquid is placed in the reaction member, it is single to reaction by 40Hz-4MHz with 50mV-10000mV sine voltage Member does frequency impedance scanning, recording responses electric current and phase difference and selects several values in scanning result, single as the reaction The characteristic parameter CS of member1；The first liquid is poured out, confining liquid is loaded in the reaction member, by 0-120 hours at 20-30 degrees Celsius, relative humidity To be placed in 70-100 space；First liquid is placed in the reaction member, it is single to reaction by 40Hz-4MHz with 50mV-10000mV sine voltage Member does frequency impedance scanning, recording responses electric current and phase difference and selects several values in scanning result, single as the reaction The characteristic parameter CS of member2；AndThe first liquid is poured out, examines thing to be put into the reaction member target for including viral antigen of various concentrations, holds Performing check, under the conditions of various concentrations, three characteristic parameter CS are obtained respectively0、CS1、CS2As the reaction under respective concentration The coating parameter of unit.
- 5. the method for detection viral antigen as claimed in claim 4, it is characterised in that it is described to pour out the first liquid, by difference The viral antigen probe solution of concentration is placed in the reaction member, by 0-120 hours at 20-30 degrees Celsius, relative humidity 70- Also include after the step of being placed in 90 space：10Hz~10MHz pumping signal is applied to the reaction member of the viral antigen probe solution containing various concentrations.
- 6. the method for detection viral antigen as claimed in claim 4, it is characterised in that the bag for obtaining the reaction member Also included the step of parameter：Obtaining characteristic parameter CS0Afterwards, cleaning liquid is placed in the reaction member to be detected, to the reaction to be detected Unit is cleaned, and the cleaning liquid includes any of deionized water, ultra-pure water, buffer solution and running water；Obtaining characteristic parameter CS1Afterwards, cleaning liquid is placed in the reaction member to be detected, to the reaction to be detected Unit is cleaned；AndObtaining characteristic parameter CS2Afterwards, cleaning liquid is placed in the reaction member to be detected, to the reaction to be detected Unit is cleaned.
- 7. the method for detection viral antigen as claimed in claim 1, it is characterised in that the method for the detection viral antigen is also Including：Thing is examined to pre-process the target that can be in the range of concentrations, the pretreatment, which includes filtering out, described can detect Target in concentration range examines the particulate matter in thing.
- 8. the method for detection viral antigen as claimed in claim 1, it is characterised in that described to determine that the target examines thing It can include the step of concentrations scope：Choose the target that concentration is ng/mL and examine thing, 1ag/mL is diluted to from ng/mL by exponential, using 10Hz~10MHz's Pumping signal examines the viral antigen in thing to carry out full scan to the target, obtains the response parameter and phase of the viral antigen The target answered examines the concentration range of thing, wherein, n >=0.5；Response parameter and corresponding target to the viral antigen examine the relation of the concentration range of thing to be fitted, and obtain and intend Close function；According to the fitting function, the concentration range that the target examines thing is obtained, and/or institute is obtained according to the fitting function State the response parameter of viral antigen.
- 9. the method for detection viral antigen as claimed in claim 1, it is characterised in that the measurement list of the reaction member connection Member work, obtaining the step of target examines the data message of viral antigen in thing includes：Apply 10Hz~10MHz pumping signal to reaction member by measuring unit, meanwhile, detect the impedance value of reaction member Change, the impedance value of the reaction member is transmitted to a memory cell and stored.
- 10. the method for detection viral antigen as claimed in claim 9, it is characterised in that in the analysis memory cell Data message, obtain testing result the step of include：Data processing, including denoising and amendment are carried out to the impedance value of the reaction member；By the impedance value of the reaction member after data processing, testing result is obtained, and the testing result is shown in one and shown Show unit.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710643983.4A CN107389935A (en) | 2017-07-31 | 2017-07-31 | The detection method and viral antigen probe method for coating of a kind of viral antigen |
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