CN107367533A - One organic molecular species detecting system and detection method of quality control - Google Patents
One organic molecular species detecting system and detection method of quality control Download PDFInfo
- Publication number
- CN107367533A CN107367533A CN201710643974.5A CN201710643974A CN107367533A CN 107367533 A CN107367533 A CN 107367533A CN 201710643974 A CN201710643974 A CN 201710643974A CN 107367533 A CN107367533 A CN 107367533A
- Authority
- CN
- China
- Prior art keywords
- reaction member
- detected
- reaction
- probe
- organic molecular
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/27—Association of two or more measuring systems or cells, each measuring a different parameter, where the measurement results may be either used independently, the systems or cells being physically associated, or combined to produce a value for a further parameter
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
Abstract
The present invention proposes an organic molecular species detecting system and detection method of quality control, the organic molecule in thing is examined to detect target by an organic molecular probe, so that detection time is shorter, accuracy of detection is higher, it is simple to operate, and it is easy to those skilled in the art to analyze the testing result of storage, reduce testing cost；Further, quality control is carried out to the reaction member for being coated with organic molecular probe in above-mentioned organic molecule detecting system, qualified reaction member can be filtered out to be detected to organic molecule, the accuracy of organic molecule detection is improved, further, by being coated with reaction member in advance, and its quality is controlled, the time of detection organic molecule is shortened, improves the sensitivity of detection, the professional technique requirement to operating personnel is also corresponding to be reduced.
Description
Technical field
The present invention relates to bioscience research field, more particularly to a kind of system for detecting organic molecule and detection quality control
Method processed.
Background technology
Organic molecule is the material base for forming biology, and that govern metabolism, nutrition and energy conversion of biology etc.
Process.The detection of organic molecule is very close with the relation of biology and medical science, especially polysaccharide, lipid, protein, nucleic acid,
The detection of proteoglycans, amino acid, nucleotides, vitamin, monose oligosaccharides and hormone etc., is controlled bioscience research and medical science
It is all significant to treat research.
Because target examines the influence of the concentration and surrounding enviroment of thing, had using existing organic molecule detecting system
The detection time length of machine Molecular Detection, complex operation, and manual record result is needed, and to the professional technique requirement of operating personnel
Higher, so as to cause, detection speed is slow and testing cost is high.
Based on this, the present invention proposes a kind of system and method for quality control for detecting organic molecule, simple to operate, detection
Precision is higher, and has carried out quality control to reaction member in the system of the detection organic molecule, screens qualified be coated with
The reaction member of organic molecular probe, improve the accuracy of detection.
The content of the invention
It is an object of the invention to provide the system of detection organic molecule and detection method of quality control so that detection time
Shorter, accuracy of detection is higher, and simple to operate, requires relatively low to the professional technique of operating personnel.
In order to achieve the above object, one aspect of the present invention provides an organic molecular species detecting system, for detecting target
Examine the organic molecule in thing, it is characterised in that including reaction member, measuring unit and memory cell；The reaction member
An organic molecular probe is coated with, the target for being opposite in the reaction member examines the organic molecule in thing to be examined
Survey, the measuring unit is used to measure the organic molecule data message in the reaction member, and is stored to the storage
Unit.
Optionally, in above-mentioned organic molecule detecting system, the reaction member includes a reaction chamber, the organic molecule
Probe is coated on a detecting electrode of the bottom of the reaction chamber, and the detecting electrode is connected with the measuring unit.
Optionally, in above-mentioned organic molecule detecting system, the organic molecular probe can be special with the organic molecule
Property react, the organic molecular probe include DNA probe, rna probe, PNA probe, synthesized dna probe, protein visit
One kind or any combination in pin and polymer probe.
Optionally, in above-mentioned organic molecule detecting system, at least one is also included on the detecting electrode of the reaction member
Layer closure.
In order to achieve the above object, another aspect of the invention provides a kind of to the progress quality control of any of the above-described reaction member
The method of system, it is characterised in that including：
Organic molecular probe is coated on the detecting electrode of a reaction member to be detected；
First liquid is placed in the reaction member, first liquid include deionized water, ultra-pure water, PBS solution,
Any of PBS-T solution and Buffer B；
One end of the reaction member to be detected is applied to 40Hz-110MHz pumping signal, described to be detected anti-
Answer the other end of unit to measure the size of current of response signal, and the phase place change relative to pumping signal, and obtained with this
The data message of reaction member to be detected, the data message include impedance, phase shifting capability, electrical conductivity, resistive component, inductance point
One in amount and capacitive component or any combination；And
The data message of a qualified reaction member is obtained using above-mentioned steps, contrasts the data letter of the qualified reaction member
Whether breath is consistent with the data message of the reaction member to be detected, if unanimously, the reaction member to be detected is qualified.
Optionally, in above-mentioned reaction member method of quality control, in addition to：
When the data message of the qualified reaction member and the inconsistent data message of the reaction member to be detected, weight
The reaction member newly is coated with using the organic molecular probe, the organic molecular probe can specificity with the organic molecule
Ground reacts, and the organic molecular probe includes DNA probe, rna probe, PNA probe, synthesized dna probe, protein probe
And one kind or any combination in polymer probe；
First liquid is placed in the reaction member to be detected；
One end of the reaction member to be detected is applied to 40Hz-110MHz pumping signal, described to be detected anti-
Answer the other end of unit to measure the size of current of response signal, and the phase place change relative to pumping signal, and obtained with this
The data message of reaction member to be detected, the data message include impedance, electrical conductivity, resistive component, inductive component and electricity
Hold one in component or any combination；And
The data message of a qualified reaction member is obtained using above-mentioned steps, contrasts the data letter of the qualified reaction member
Whether breath is consistent with the data message of the reaction member to be detected, if unanimously, the reaction member to be detected is qualified；If no
Unanimously, repeat the above steps, until the reaction member to be detected is qualified.
Optionally, in above-mentioned reaction member method of quality control, reuse described in the organic molecular probe coating
The step of reaction member, includes：
First liquid is placed in reaction member to be detected, 40Hz-4MHz pairs is pressed with 50mV-10000mV sine voltage
Each reaction member does the scanning of frequency electrical conductivity, recording responses electric current and phase difference and selects several values in scanning result,
Characteristic parameter CS as the reaction member to be detected0；
The first liquid is poured out, the organic molecular probe solution of the first concentration is placed in inside reaction member to be detected, by 0-
At 10-40 degrees Celsius, relative humidity is to be placed in 70-90 space, and the organic molecular probe solution includes DNA within 120 hours
Probe, rna probe, PNA probe, synthesized dna probe, protein probe and polymer probe solution in one kind or any
Combination；First concentration is corresponding with the concentration of the target detection thing；
First liquid is placed in reaction member to be detected, 40Hz-4MHz pairs is pressed with 50mV-10000mV sine voltage
Reaction member does frequency impedance scanning, recording responses electric current and phase difference and selects several values in scanning result, is used as this
The characteristic parameter CS of reaction member1；
The first liquid is poured out, confining liquid is loaded in reaction member to be detected, by 0-120 hours at 20-30 degrees Celsius, phase
To humidity be 70-100 space in place；
First liquid is placed in reaction member to be detected, 40Hz-4MHz pairs is pressed with 50mV-10000mV sine voltage
Reaction member does frequency impedance scanning, recording responses electric current and phase difference and selects several values in scanning result, is used as this
The characteristic parameter CS of reaction member2；And
The first liquid is poured out, examines thing to be put into reaction to be detected the target of known the first concentration for including organic molecule
In unit, inspection is performed, obtains three coating parameter CS respectively0、CS1、CS2Bag as the reaction member under the first concentration
By parameter, standing time, temperature and humidity corresponding to each parameter are obtained.
Optionally, in above-mentioned reaction member method of quality control, the first liquid is poured out, by the organic molecule of the first concentration
Probe solution is placed in inside the reaction member to be detected, and by 0-120 hours at 20-30 degrees Celsius, relative humidity is 70-90's
Also include after the step of being placed in space：
10Hz~10MHz excitation is applied to the reaction member to be detected of the organic molecular probe solution containing the first concentration
Signal.
Optionally, in above-mentioned reaction member method of quality control, reuse described in the organic molecular probe coating
The step of reaction member to be detected, also includes：
Obtaining characteristic parameter CS0Afterwards, cleaning liquid is placed in the reaction member to be detected, to described to be detected
Reaction member is cleaned, and the cleaning liquid includes any of deionized water, ultra-pure water, buffer solution and running water；
Obtaining characteristic parameter CS1Afterwards, cleaning liquid is placed in the reaction member to be detected, to described to be detected
Reaction member is cleaned；And
Obtaining characteristic parameter CS2Afterwards, cleaning liquid is placed in the reaction member to be detected, to described to be detected
Reaction member is cleaned.
In summary, the present invention proposes an organic molecular species detecting system, by being coated with one in advance in the reaction cell
Organic molecular probe with to target examine thing in organic molecule detect so that detection time is shorter, and accuracy of detection is higher,
And the professional technique of operating personnel is required to reduce, it is simple to operate；Further, target inspection thing is placed in the reaction member
In, it is increased with the organic molecular probe contact area, add detection accuracy；Measured by measuring unit described anti-
The organic molecule data message in unit to be answered, and data message is stored to memory cell, testing result is easy to achieve, and
It is easy to those skilled in the art to analyze the testing result of storage, reduces testing cost.
Brief description of the drawings
Fig. 1 is the organic molecule detecting system structural representation in one embodiment of the present invention；
Fig. 2 is the flow chart for the method that quality control is carried out to the reaction member of embodiment in Fig. 1；
Fig. 3 is the method flow that coating organic molecular probe is re-started when the reaction member of embodiment in Fig. 2 is unqualified
Figure.
Specifically, 1- measuring units；11- signaling modules；12 detection modules；2- memory cell；3- reaction members.
Embodiment
The embodiment of the present invention is described in more detail below in conjunction with schematic diagram.According to description below and
Claims, advantages and features of the invention will become apparent from.It should be noted that accompanying drawing is using very simplified form and
Using non-accurately ratio, only for the purpose of facilitating and clarifying the purpose of the embodiments of the invention.
The present invention proposes an organic molecular species detecting system, for detecting the organic molecule in target inspection thing, reference chart
1, including reaction member 3, measuring unit 1 and memory cell 2；The reaction member 3 is coated with an organic molecular probe, is used for
The target being opposite in the reaction member 3 examines the organic molecule in thing to be detected, and the measuring unit 1 is used to measure institute
The organic molecule data message in reaction member 3 is stated, and is stored to the memory cell 2.
Preferably, the reaction member 3 in the present embodiment includes a reaction chamber, and the organic molecular probe is coated in described anti-
Answer on a detecting electrode of the bottom of chamber, the detecting electrode is connected with the measuring unit 1.In detection process, target is examined
Thing reacts with the organic molecular probe in the reaction chamber of reaction member 3, and measuring unit 1 obtains to the reaction
Organic molecule data message measures, and is stored into the memory cell 2.
Optionally, the data message of the organic molecule in the present invention includes but is not limited to the impedance value of the reaction member
The impedance value of the reaction member is transmitted to a memory cell 2 and stored by change, detection module 12.Organic molecule with it is organic
Molecular probe reaction changes the thickness of the detecting electrode of the reaction member, and therefore, the impedance value of reaction member can change,
Preferably, in one embodiment of the invention, the change in impedance value of reaction member 3 is detected, the impedance value of the reaction member 3 is transmitted
Stored to a memory cell 2.
Optionally, those skilled in the art can analyze and obtain according to the change in impedance value of the reaction member 3 in the present embodiment
Testing result, organic molecule species that the testing result includes but is not limited to examine for the target in thing, quantity and normal
Situation etc..
Specifically, the reaction member is coated with organic molecular probe, the organic molecular probe and the organic molecule
Can specifically it react, the organic molecular probe includes DNA probe, rna probe, PNA probe, synthesized dna probe, egg
One kind or any combination in white matter probe and polymer probe, the present invention do not do any restrictions to this.For example, one mesh of detection
Active polysaccharide-lentinan more in detectable substance is marked, then the lentinan is detected from lentinan probe, institute
State lentinan probe can specifically to react with the lentinan in the target detection thing, so as to change described harden
The impedance value of unit 3.
Optionally, at least one layer of closure is also included on the detecting electrode of the reaction member 3.The closure is typically adopted
Prepared with unrelated protein, prevent other materials to be adsorbed onto on detecting electrode and testing result is impacted, to improve the standard of detection
True property.
Specifically, with reference to figure 2, quality control is carried out to the reaction member in above-mentioned organic molecule detecting system, can be sieved
Qualified reaction member is selected to be detected to organic molecule, the accuracy of organic molecule detection is improved, further, by advance
Organic molecular probe is coated with, and its quality is controlled, the time of detection organic molecule is shortened, to the specialty of operating personnel
Technical requirements also accordingly reduce.
Specifically, the method for quality control is carried out to the reaction member in above-mentioned organic molecule detecting system to be included：
Step S1：Organic molecular probe is coated on the detecting electrode of a reaction member to be detected；
Step S2：First liquid is placed in the reaction member, first liquid includes deionized water, ultra-pure water, PBS
Any of solution, PBS-T solution and Buffer B；
Step S3：One end of the reaction member to be detected is applied to 40Hz-110MHz pumping signal, is treated described
The size of current of the other end measurement response signal of detection reaction member, and the phase place change relative to pumping signal, and with
This obtains the data message of reaction member to be detected；
Optionally, the data message includes impedance, phase shifting capability, electrical conductivity, resistive component, inductive component and electric capacity
One in component or any combination；The present invention does not do any restrictions to this；
Step S4：The data message of a qualified reaction member is obtained by step S1-S3, contrasts the qualified reaction member
Data message it is whether consistent with the data message of the reaction member to be detected, if unanimously, the reaction member to be detected
It is qualified.
Optionally, in one embodiment of the present invention, when the qualified reaction member data message with it is described to be detected
When the data message of reaction member is inconsistent, reuses organic molecular probe solution and be coated with the reaction member, it is described organic
Molecular probe solution includes DNA probe, rna probe, PNA probe, synthesized dna probe, protein probe and polymer probe
Solution in one kind or any combination, the present invention this is not intended to be limited in any；
First liquid is placed in the reaction member to be detected；
One end of the reaction member to be detected is applied to 40Hz-110MHz pumping signal, described to be detected anti-
Answer the other end of unit to measure the size of current of response signal, and the phase place change relative to pumping signal, and obtained with this
The data message of reaction member to be detected, the data message include impedance, electrical conductivity, resistive component, inductive component and electricity
Hold one in component or any combination；
The data message of a qualified reaction member is obtained using above-mentioned steps, contrasts the data letter of the qualified reaction member
Whether breath is consistent with the data message of the reaction member to be detected, if unanimously, the reaction member to be detected is qualified；If no
Unanimously, repeat the above steps, until the reaction member to be detected is qualified.
Optionally, in one embodiment of the present invention, with reference to figure 3, reuse described in the organic molecular probe coating
Reaction member to be detected comprises the following steps.
Step Sa：First liquid is placed in reaction member to be detected, 40Hz- is pressed with 50mV-10000mV sine voltage
4MHz does the scanning of frequency electrical conductivity, recording responses electric current and phase difference to each reaction member and selects several of scanning result
Value, the characteristic parameter CS as the reaction member to be detected0；
Step Sb：The first liquid is poured out, the organic molecular probe solution of the first concentration is placed in reaction member to be detected
Portion, by 0-120 hours at 20-30 degrees Celsius, relative humidity is to be placed in 70-90 space, the organic molecular probe solution
One in solution including DNA probe, rna probe, PNA probe, synthesized dna probe, protein probe and polymer probe
Kind or any combination；Optionally, the organic molecular probe solution for placing 0 hour as the first concentration is placed in reaction member to be detected
Internal moment.First concentration is corresponding with the concentration of the target detection thing.
In one embodiment of the invention, the organic molecule is entered by the change of the impedance value of the reaction member to be detected
Row detection；Optionally, those skilled in the art can by extracting the product of organic molecule and organic molecular probe specific reaction,
Composition, species and concentration of organic molecule in the target inspection thing etc. is obtained, is further studied.
Step Sc：First liquid is placed in reaction member to be detected, 40Hz- is pressed with 50mV-10000mV sine voltage
4MHz does frequency impedance scanning, recording responses electric current and phase difference to reaction member and selects several values of scanning result, makees
For the characteristic parameter CS of the reaction member to be detected1；
Step Sd：The first liquid is poured out, confining liquid is loaded in reaction member to be detected, is taken the photograph by 0-120 hours in 20-30
Family name's degree, relative humidity are to be placed in 70-100 space；
Step Se：First liquid is placed in reaction member to be detected, 40Hz- is pressed with 50mV-10000mV sine voltage
4MHz does frequency impedance scanning, recording responses electric current and phase difference to reaction member and selects several values of scanning result, makees
For the characteristic parameter CS of the reaction member to be detected2；
Step Sf：The first liquid is poured out, examines thing to be put into the target of known the first concentration for including organic molecule and treats
Detect in reaction member, perform inspection, obtain three coating parameter CS respectively0、CS1、CS2As reaction member under the concentration
Parameter is coated with, obtains standing time, temperature and humidity corresponding to each parameter.
Optionally, step Sg is also included after Sb steps：To the to be checked of the organic molecular probe solution containing the first concentration
Survey the pumping signal that reaction member applies 10Hz~10MHz.It is easy to be internally formed significant thermograde in reaction member and draw
The local fluid flow risen, coating speed of the increase organic molecular probe solution on detecting electrode, the present invention do not appoint to this
What is limited.
Optionally, in one embodiment of the invention, it is single to reuse the organic molecular probe coating reaction to be detected
The step of member also includes：
Obtaining characteristic parameter CS0Afterwards, cleaning liquid is placed in the reaction member to be detected, to described to be detected
Reaction member is cleaned, and the cleaning liquid includes any of deionized water, ultra-pure water, buffer solution and running water；
Obtaining characteristic parameter CS1Afterwards, cleaning liquid is placed in the reaction member to be detected, to described to be detected
Reaction member is cleaned；And
Obtaining characteristic parameter CS2Afterwards, cleaning liquid is placed in the reaction member to be detected, to described to be detected
Reaction member is cleaned.
Reaction member is cleaned by cleaning liquid, other particulate matters or ion in reaction member is filtered, can increase
Add coating precision, so as to improve the accuracy of detection of reaction member.
The target for being applied to the first concentration by filtering out examines the reaction member of thing, and the target of first concentration is examined
The organic molecule tested in thing is detected, and is on the one hand coated with organic molecular probe to reaction member in advance, is reduced detection time,
On the other hand, the qualified reaction member selected by step Sa- steps Sf, can obtain corresponding standing time, temperature and
Humidity, increase the accuracy of detection, meanwhile, testing process is simple, the professional technique of operating personnel is required to reduce, so as to reduce
Testing cost, has a feasibility.
In summary, the present invention proposes an organic molecular species detecting system, by being coated with one in advance in the reaction cell
Organic molecular probe with to target examine thing in organic molecule detect so that detection time is shorter, and accuracy of detection is higher,
And the professional technique of operating personnel is required to reduce, it is simple to operate；Further, target inspection thing is placed in the reaction member
In, it is increased with the organic molecular probe contact area, add detection accuracy；Measured by measuring unit described anti-
The organic molecule data message in unit to be answered, and data message is stored to memory cell, testing result is easy to achieve, and
It is easy to those skilled in the art to analyze the testing result of storage, reduces testing cost.
The preferred embodiments of the present invention are above are only, any restrictions effect is not played to the present invention.Belonging to any
Those skilled in the art, in the range of technical scheme is not departed from, to the invention discloses technical scheme and
Technology contents make the variation such as any type of equivalent substitution or modification, belong to the content without departing from technical scheme, still
Belong within protection scope of the present invention.
Claims (9)
- A 1. organic molecular species detecting system, for detecting the organic molecule in target inspection thing, it is characterised in that including reaction Unit, measuring unit and memory cell；The reaction member is coated with an organic molecular probe, for being opposite to the reaction Target in unit examines the organic molecule in thing to be detected, and the measuring unit is used to measure having in the reaction member Machine molecular data information, and stored to the memory cell.
- 2. organic molecule detecting system as claimed in claim 1, it is characterised in that the reaction member includes a reaction chamber, The organic molecular probe is coated on a detecting electrode of the bottom of the reaction chamber, and the detecting electrode and the measurement are single Member connection.
- 3. organic molecule detecting system as claimed in claim 2, it is characterised in that the organic molecular probe with it is described organic Molecule can specifically react, and the organic molecular probe includes DNA probe, rna probe, PNA probe, synthetic DNA and visited One kind or any combination in pin, protein probe and polymer probe.
- 4. organic molecule detecting system as claimed in claim 2, it is characterised in that on the detecting electrode of the reaction member also Including at least one layer of closure.
- 5. a kind of method that any reaction member to as described in claim 1-4 carries out quality control, it is characterised in that bag Include：Organic molecular probe is coated on the detecting electrode of a reaction member to be detected；First liquid is placed in the reaction member, first liquid includes deionized water, ultra-pure water, PBS solution, PBS-T Any of solution and Buffer B；One end of the reaction member to be detected is applied to 40Hz-110MHz pumping signal, it is single in the reaction to be detected The size of current of the other end measurement response signal of member, and the phase place change relative to pumping signal, and obtained with this to be checked Survey reaction member data message, the data message include impedance, phase shifting capability, electrical conductivity, resistive component, inductive component with And one in capacitive component or any combination；AndThe data message of a qualified reaction member is obtained using above-mentioned steps, contrast the data message of the qualified reaction member with Whether the data message of the reaction member to be detected is consistent, if unanimously, the reaction member to be detected is qualified.
- 6. method of quality control as claimed in claim 5, it is characterised in that also include：When the data message of the qualified reaction member and the inconsistent data message of the reaction member to be detected, make again The reaction member is coated with the organic molecular probe, the organic molecular probe can specifically be sent out with the organic molecule Raw reaction, the organic molecular probe include DNA probe, rna probe, PNA probe, synthesized dna probe, protein probe and One kind or any combination in polymer probe；First liquid is placed in the reaction member to be detected；One end of the reaction member to be detected is applied to 40Hz-110MHz pumping signal, it is single in the reaction to be detected The size of current of the other end measurement response signal of member, and the phase place change relative to pumping signal, and obtained with this to be checked The data message of reaction member is surveyed, the data message includes impedance, electrical conductivity, resistive component, inductive component and electric capacity point One in amount or any combination；AndThe data message of a qualified reaction member is obtained using above-mentioned steps, contrast the data message of the qualified reaction member with Whether the data message of the reaction member to be detected is consistent, if unanimously, the reaction member to be detected is qualified；If differ Cause, repeat the above steps, until the reaction member to be detected is qualified.
- 7. method of quality control as claimed in claim 6, it is characterised in that reuse the organic molecular probe coating institute The step of stating reaction member to be detected includes：First liquid is placed in reaction member to be detected, with 50mV-10000mV sine voltage by 40Hz-4MHz to each Reaction member does the scanning of frequency electrical conductivity, recording responses electric current and phase difference and selects several values in scanning result, as The characteristic parameter CS of the reaction member to be detected0；The first liquid is poured out, the organic molecular probe solution of the first concentration is placed in inside reaction member to be detected, it is small by 0-120 When at 10-40 degrees Celsius, relative humidity is to be placed in 70-90 space, the organic molecular probe solution including DNA probe, Rna probe, PNA probe, synthesized dna probe, protein probe and polymer probe solution in one kind or any combination； First concentration is corresponding with the concentration of the target detection thing；First liquid is placed in reaction member to be detected, with 50mV-10000mV sine voltage by 40Hz-4MHz to reaction Unit does frequency impedance scanning, recording responses electric current and phase difference and selects several values in scanning result, as the reaction The characteristic parameter CS of unit1；The first liquid is poured out, confining liquid is loaded in reaction member to be detected, it is relatively wet by 0-120 hours at 20-30 degrees Celsius Spend in the space for 70-100 and place；First liquid is placed in reaction member to be detected, with 50mV-10000mV sine voltage by 40Hz-4MHz to reaction Unit does frequency impedance scanning, recording responses electric current and phase difference and selects several values in scanning result, as the reaction The characteristic parameter CS of unit2；AndThe first liquid is poured out, examines thing to be put into reaction member to be detected the target of known the first concentration for including organic molecule It is interior, inspection is performed, obtains three coating parameter CS respectively0、CS1、CS2Coating ginseng as the reaction member under the first concentration Number, obtains standing time, temperature and humidity corresponding to each parameter.
- 8. method of quality control as claimed in claim 7, it is characterised in that the first liquid is poured out, by the organic of the first concentration Molecular probe solution is placed in inside the reaction member to be detected, by 0-120 hours at 20-30 degrees Celsius, relative humidity 70- Also include after the step of being placed in 90 space：10Hz~10MHz excitation letter is applied to the reaction member to be detected of the organic molecular probe solution containing the first concentration Number.
- 9. method of quality control as claimed in claim 7, it is characterised in that reuse the organic molecular probe coating institute The step of stating reaction member to be detected also includes：Obtaining characteristic parameter CS0Afterwards, cleaning liquid is placed in the reaction member to be detected, to the reaction to be detected Unit is cleaned, and the cleaning liquid includes any of deionized water, ultra-pure water, buffer solution and running water；Obtaining characteristic parameter CS1Afterwards, cleaning liquid is placed in the reaction member to be detected, to the reaction to be detected Unit is cleaned；AndObtaining characteristic parameter CS2Afterwards, cleaning liquid is placed in the reaction member to be detected, to the reaction to be detected Unit is cleaned.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710643974.5A CN107367533A (en) | 2017-07-31 | 2017-07-31 | One organic molecular species detecting system and detection method of quality control |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710643974.5A CN107367533A (en) | 2017-07-31 | 2017-07-31 | One organic molecular species detecting system and detection method of quality control |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107367533A true CN107367533A (en) | 2017-11-21 |
Family
ID=60309086
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710643974.5A Pending CN107367533A (en) | 2017-07-31 | 2017-07-31 | One organic molecular species detecting system and detection method of quality control |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107367533A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112986336A (en) * | 2021-05-12 | 2021-06-18 | 佛山微奥云生物技术有限公司 | Buffer solution and application thereof |
| CN112986205A (en) * | 2021-05-12 | 2021-06-18 | 佛山微奥云生物技术有限公司 | Fluorescence and chemiluminescence detection method based on electrical acceleration |
| CN112986688A (en) * | 2021-05-12 | 2021-06-18 | 佛山微奥云生物技术有限公司 | Characterization method for producing rapid detection chip |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103940987A (en) * | 2014-04-10 | 2014-07-23 | 山东理工大学 | Rapid detector for simultaneously detecting aflatoxin and pesticides in peanuts |
| CN103940854A (en) * | 2014-04-10 | 2014-07-23 | 山东理工大学 | Rapid detector capable of simultaneously detecting pesticide and heavy metal residues in fruits and vegetables |
| CN104165906A (en) * | 2014-07-14 | 2014-11-26 | 中国农业大学 | Virus detector |
| CN105572178A (en) * | 2015-12-18 | 2016-05-11 | 深圳市前海康启源科技有限公司 | Edible meat freshness detection apparatus and method |
| CN106324057A (en) * | 2016-08-01 | 2017-01-11 | 红河学院 | Method for detecting aflatoxin through immunosensor |
-
2017
- 2017-07-31 CN CN201710643974.5A patent/CN107367533A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103940987A (en) * | 2014-04-10 | 2014-07-23 | 山东理工大学 | Rapid detector for simultaneously detecting aflatoxin and pesticides in peanuts |
| CN103940854A (en) * | 2014-04-10 | 2014-07-23 | 山东理工大学 | Rapid detector capable of simultaneously detecting pesticide and heavy metal residues in fruits and vegetables |
| CN104165906A (en) * | 2014-07-14 | 2014-11-26 | 中国农业大学 | Virus detector |
| CN105572178A (en) * | 2015-12-18 | 2016-05-11 | 深圳市前海康启源科技有限公司 | Edible meat freshness detection apparatus and method |
| CN106324057A (en) * | 2016-08-01 | 2017-01-11 | 红河学院 | Method for detecting aflatoxin through immunosensor |
Non-Patent Citations (1)
| Title |
|---|
| XIAOZHU LIU等: "A low cost and palm-size analyzer for rapid and sensitive protein detection by AC electrokinetics capacitive sensing", 《BIOSENSORS AND BIOELECTRONICS》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112986336A (en) * | 2021-05-12 | 2021-06-18 | 佛山微奥云生物技术有限公司 | Buffer solution and application thereof |
| CN112986205A (en) * | 2021-05-12 | 2021-06-18 | 佛山微奥云生物技术有限公司 | Fluorescence and chemiluminescence detection method based on electrical acceleration |
| CN112986688A (en) * | 2021-05-12 | 2021-06-18 | 佛山微奥云生物技术有限公司 | Characterization method for producing rapid detection chip |
| CN112986688B (en) * | 2021-05-12 | 2021-08-27 | 佛山微奥云生物技术有限公司 | Characterization method for producing rapid detection chip |
| CN112986205B (en) * | 2021-05-12 | 2021-08-27 | 佛山微奥云生物技术有限公司 | Fluorescence and chemiluminescence detection method based on electrical acceleration |
| WO2022237398A1 (en) * | 2021-05-12 | 2022-11-17 | 佛山微奥云生物技术有限公司 | Electrical acceleration-based method for detecting fluorescence and chemiluminescence |
| US11874275B2 (en) | 2021-05-12 | 2024-01-16 | Foshan Microwonders Biotechnology Co., Ltd. | Method for fluorescent immunoassay (FIA) and chemiluminescent immunoassay (CLIA) based on electrokinetic acceleration |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN203772786U (en) | Chip type interdigital array electrode impedance sensor | |
| CN107367533A (en) | One organic molecular species detecting system and detection method of quality control | |
| CN107533030A (en) | The sequencing based on nano-pore stimulated using the voltage of change | |
| Šalplachta et al. | Latest improvements in CIEF: from proteins to microorganisms | |
| CN103827662A (en) | Microcantilever sensor with bimorph actuation and piezoresistive read-out | |
| CN108139382A (en) | Change to reduce the encoding state of the nano-pore of size of data | |
| Checa et al. | Fast label‐free nanoscale composition mapping of eukaryotic cells via scanning dielectric force volume microscopy and machine learning | |
| CN108982605A (en) | A kind of endotoxin aptamer sensor and its endotoxic method of detection based on copper-rich ionic material label | |
| CN102782496B (en) | Method and apparatus for forming of an automated sampling device for the detection of salmonella enterica utilizing an electrochemical aptamer biosensor | |
| JP2010513861A (en) | Wet and sensitive surface microelectronic devices | |
| CN101858881A (en) | Sensor for detecting penicillin in liquid | |
| CN107389757A (en) | A kind of DNA detecting systems and detection method of quality control | |
| CN106442665B (en) | A kind of preparation method of the Ratio-type aptamer sensor based on screen printing electrode detection antibiotic residue | |
| CN105021499B (en) | Utilize the method for visualizing of fluid diffusion in nuclear magnetic resonance evaluation porous media | |
| Cui et al. | Rapid detection of progesterone by commercially available microelectrode chips | |
| CN107367532A (en) | A kind of microbial metabolic products detecting system and detection method of quality control | |
| CN107271497A (en) | A kind of protein antibody detecting system and detection method of quality control | |
| CN107389739A (en) | A kind of RNA detecting systems and detection method of quality control | |
| Hazarika et al. | PC-based instrumentation system for the detection of moisture content of tea leaves at its final stage | |
| CN107389752A (en) | A kind of bacterial antigens detecting system and detection method of quality control | |
| CN107422118A (en) | A kind of virus antigen detection system and detection method of quality control | |
| CN108051648A (en) | A kind of material electromagnetic attributes measuring method based on DC potential and detection method of eddy | |
| CN107271503A (en) | The detection method and organic molecular probe method for coating of one organic molecular species | |
| CN107367541A (en) | A kind of microorganism detection system and detection method of quality control | |
| CN107271496A (en) | The detection method and microorganism probe method for coating of a kind of microorganism |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20171121 |