CN107389751A - A kind of antiviral antibody detecting system and detection method of quality control - Google Patents
A kind of antiviral antibody detecting system and detection method of quality control Download PDFInfo
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- CN107389751A CN107389751A CN201710642050.3A CN201710642050A CN107389751A CN 107389751 A CN107389751 A CN 107389751A CN 201710642050 A CN201710642050 A CN 201710642050A CN 107389751 A CN107389751 A CN 107389751A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
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Abstract
The present invention proposes a kind of antiviral antibody detecting system and detection method of quality control, the antiviral antibody in thing is examined to detect target by an antiviral antibody probe, so that detection time is shorter, accuracy of detection is higher, it is simple to operate, and it is easy to those skilled in the art to analyze the testing result of storage, reduce testing cost；Further, quality control is carried out to the reaction member for being coated with antiviral antibody probe in above-mentioned antiviral antibody detecting system, qualified reaction member can be filtered out to be detected to antiviral antibody, the accuracy of antiviral antibody detection is improved, further, by being coated with reaction member in advance, and its quality is controlled, the time of detection antiviral antibody is shortened, improves the sensitivity of detection, the professional technique requirement to operating personnel is also corresponding to be reduced.
Description
Technical field
The present invention relates to biological technical field, more particularly to a kind of system for detecting antiviral antibody and detection quality control side
Method.
Background technology
Virus is the acellular form being made up of a nucleic acid molecules and protein, by parasitic life between life entity and
Organic species between non-life body, it, which can infect, nearly all has cyto-architectural life entity.
Clinical manifestation caused by the infection of virus can not be distinguished in time in clinical or experimentation, it is therefore desirable to quick inspection
Survey species and the cycle of virus, in order to aid in case control, and take effective control measure, at present, it is more using using Electronic Speculum,
Immuno-electron microscope, immunofluorescence technique, ELISA and radioimmunology etc. can directly check virion and virus in sample
Antibody, it is commonly used for quick and early diagnosis.
Because target examines the influence of the concentration and surrounding enviroment of thing, disease is carried out using existing antiviral antibody detection method
The detection time length of malicious antibody test, complex operation, and manual record result is needed, and to the professional technique requirement of operating personnel
Higher, so as to cause, detection speed is slow and testing cost is high.
Based on this, the present invention proposes a kind of system and method for quality control for detecting antiviral antibody, simple to operate, detection
Precision is higher, and has carried out quality control to reaction member in the system of the detection antiviral antibody, screens qualified be coated with
The reaction member of antiviral antibody probe, improve the accuracy of detection.
The content of the invention
It is an object of the invention to provide the system of detection antiviral antibody and detection method of quality control so that detection time
Shorter, accuracy of detection is higher, and simple to operate, requires relatively low to the professional technique of operating personnel.
In order to achieve the above object, one aspect of the present invention provides a kind of antiviral antibody detecting system, for detecting target
Examine the antiviral antibody in thing, it is characterised in that including reaction member, measuring unit and memory cell；The reaction member
An antiviral antibody probe is coated with, the target for being opposite in the reaction member examines the antiviral antibody in thing to be examined
Survey, the measuring unit is used to measure the antiviral antibody data message in the reaction member, and is stored to the storage
Unit.
Optionally, in above-mentioned antiviral antibody detecting system, the reaction member includes a reaction chamber, the antiviral antibody
Probe is coated on a detecting electrode of the bottom of the reaction chamber, and the detecting electrode is connected with the measuring unit.
Optionally, in above-mentioned antiviral antibody detecting system, the antiviral antibody probe causes including the antiviral antibody
Specific antibody, the specific antibody includes neutralizing antibody, complement fixation antibody and hemagglutination inhibition antibody.
Optionally, in above-mentioned antiviral antibody detecting system, at least one is also included on the detecting electrode of the reaction member
Layer closure.
In order to achieve the above object, another aspect of the invention provides a kind of to the progress quality control of any of the above-described reaction member
The method of system, it is characterised in that including：
Antiviral antibody probe is coated on the detecting electrode of a reaction member to be detected；
First liquid is placed in the reaction member, first liquid include deionized water, ultra-pure water, PBS solution,
Any of PBS-T solution and Buffer B；
One end of the reaction member to be detected is applied to 40Hz-110MHz pumping signal, described to be detected anti-
Answer the other end of unit to measure the size of current of response signal, and the phase place change relative to pumping signal, and obtained with this
The data message of reaction member to be detected, the data message include impedance, phase shifting capability, electrical conductivity, resistive component, inductance point
One in amount and capacitive component or any combination；And
The data message of a qualified reaction member is obtained using above-mentioned steps, contrasts the data letter of the qualified reaction member
Whether breath is consistent with the data message of the reaction member to be detected, if unanimously, the reaction member to be detected is qualified.
Optionally, in above-mentioned reaction member method of quality control, in addition to：
When the data message of the qualified reaction member and the inconsistent data message of the reaction member to be detected, weight
The reaction member newly is coated with using the antiviral antibody probe, the antiviral antibody probe is included caused by the antiviral antibody
Specific antibody, the specific antibody include neutralizing antibody, complement fixation antibody and hemagglutination inhibition antibody；
First liquid is placed in the reaction member to be detected；
One end of the reaction member to be detected is applied to 40Hz-110MHz pumping signal, described to be detected anti-
Answer the other end of unit to measure the size of current of response signal, and the phase place change relative to pumping signal, and obtained with this
The data message of reaction member to be detected, the data message include impedance, electrical conductivity, resistive component, inductive component and electricity
Hold one in component or any combination；And
The data message of a qualified reaction member is obtained using above-mentioned steps, contrasts the data letter of the qualified reaction member
Whether breath is consistent with the data message of the reaction member to be detected, if unanimously, the reaction member to be detected is qualified；If no
Unanimously, repeat the above steps, until the reaction member to be detected is qualified.
Optionally, in above-mentioned reaction member method of quality control, reuse described in the antiviral antibody probe coating
The step of reaction member, includes：
First liquid is placed in reaction member to be detected, 40Hz-4MHz pairs is pressed with 50mV-10000mV sine voltage
Each reaction member does the scanning of frequency electrical conductivity, recording responses electric current and phase difference and selects several values in scanning result,
Characteristic parameter CS as the reaction member to be detected0；
The first liquid is poured out, the antiviral antibody probe solution of the first concentration is placed in inside reaction member to be detected, by 0-
At 10-40 degrees Celsius, relative humidity is to be placed in 70-90 space, and the antiviral antibody probe solution includes and institute within 120 hours
Specific antigen solution corresponding to antiviral antibody is stated, the specific antigen solution includes neutralization antigenic solution, complement is combined and resisted
Original solution and blood clotting suppress antigenic solution；
First concentration is corresponding with the concentration of the target detection thing；
First liquid is placed in reaction member to be detected, 40Hz-4MHz pairs is pressed with 50mV-10000mV sine voltage
Reaction member does frequency impedance scanning, recording responses electric current and phase difference and selects several values in scanning result, is used as this
The characteristic parameter CS of reaction member1；
The first liquid is poured out, confining liquid is loaded in reaction member to be detected, by 0-120 hours at 20-30 degrees Celsius, phase
To humidity be 70-100 space in place；
First liquid is placed in reaction member to be detected, 40Hz-4MHz pairs is pressed with 50mV-10000mV sine voltage
Reaction member does frequency impedance scanning, recording responses electric current and phase difference and selects several values in scanning result, is used as this
The characteristic parameter CS of reaction member2；And
The first liquid is poured out, examines thing to be put into reaction to be detected the target of known the first concentration for including antiviral antibody
In unit, inspection is performed, obtains three coating parameter CS respectively0、CS1、CS2Bag as the reaction member under the first concentration
By parameter, standing time, temperature and humidity corresponding to each parameter are obtained.
Optionally, in above-mentioned reaction member method of quality control, the first liquid is poured out, by the antiviral antibody of the first concentration
Probe solution is placed in inside the reaction member to be detected, and by 0-120 hours at 20-30 degrees Celsius, relative humidity is 70-90's
Also include after the step of being placed in space：
10Hz~10MHz excitation is applied to the reaction member to be detected of the antiviral antibody probe solution containing the first concentration
Signal.
Optionally, in above-mentioned reaction member method of quality control, reuse described in the antiviral antibody probe coating
The step of reaction member to be detected, also includes：
Obtaining characteristic parameter CS0Afterwards, cleaning liquid is placed in the reaction member to be detected, to described to be detected
Reaction member is cleaned, and the cleaning liquid includes any of deionized water, ultra-pure water, buffer solution and running water；
Obtaining characteristic parameter CS1Afterwards, cleaning liquid is placed in the reaction member to be detected, to described to be detected
Reaction member is cleaned；And
Obtaining characteristic parameter CS2Afterwards, cleaning liquid is placed in the reaction member to be detected, to described to be detected
Reaction member is cleaned.
In summary, the present invention proposes a kind of antiviral antibody detecting system, by being coated with one in advance in the reaction cell
Antiviral antibody probe with to target examine thing in antiviral antibody detect so that detection time is shorter, and accuracy of detection is higher,
And the professional technique of operating personnel is required to reduce, it is simple to operate；Further, target inspection thing is placed in the reaction member
In, it is increased with the antiviral antibody probe contact area, add detection accuracy；Measured by measuring unit described anti-
The antiviral antibody data message in unit to be answered, and data message is stored to memory cell, testing result is easy to achieve, and
It is easy to those skilled in the art to analyze the testing result of storage, reduces testing cost.
Brief description of the drawings
Fig. 1 is the antiviral antibody detecting system structural representation in one embodiment of the present invention；
Fig. 2 is the flow chart for the method that quality control is carried out to the reaction member of embodiment in Fig. 1；
Fig. 3 is the method flow that coating antiviral antibody probe is re-started when the reaction member of embodiment in Fig. 2 is unqualified
Figure.
Specifically, 1- measuring units；11- signaling modules；12 detection modules；2- memory cell；3- reaction members.
Embodiment
The embodiment of the present invention is described in more detail below in conjunction with schematic diagram.According to description below and
Claims, advantages and features of the invention will become apparent from.It should be noted that accompanying drawing is using very simplified form and
Using non-accurately ratio, only for the purpose of facilitating and clarifying the purpose of the embodiments of the invention.
The present invention proposes a kind of antiviral antibody detecting system, for detecting the antiviral antibody in target inspection thing, reference chart
1, including reaction member 3, measuring unit 1 and memory cell 2；The reaction member 3 is coated with an antiviral antibody probe, is used for
The target being opposite in the reaction member 3 examines the antiviral antibody in thing to be detected, and the measuring unit 1 is used to measure institute
The antiviral antibody data message in reaction member 3 is stated, and is stored to the memory cell 2.
Preferably, the reaction member 3 in the present embodiment includes a reaction chamber, and the antiviral antibody probe is coated in described anti-
Answer on a detecting electrode of the bottom of chamber, the detecting electrode is connected with the measuring unit 1.In detection process, target is examined
Thing reacts with the antiviral antibody probe in the reaction chamber of reaction member 3, and measuring unit 1 obtains to the reaction
Antiviral antibody data message measures, and is stored into the memory cell 2.
Optionally, the data message of the antiviral antibody in the present invention includes but is not limited to the impedance value of the reaction member
The impedance value of the reaction member is transmitted to a memory cell 2 and stored by change, detection module 12.Antiviral antibody with it is described
Immune response occurs for antiviral antibody probe, changes the thickness of the detecting electrode of the reaction member, therefore, the resistance of reaction member
Anti- value can change, it is preferred that in one embodiment of the invention, the change in impedance value of reaction member 3 is detected, the reaction is single
The impedance value of member 3, which is transmitted to a memory cell 2, to be stored.
Optionally, those skilled in the art can analyze and obtain according to the change in impedance value of the reaction member 3 in the present embodiment
Testing result, antiviral antibody species that the testing result includes but is not limited to examine for the target in thing, quantity and normal
Situation etc.；It is right according to composition of antiviral antibody etc. described in the response analysis to the antiviral antibody and specific antigen, the present invention
This does not do any restrictions.
Specifically, the reaction member is coated with antiviral antibody probe, and the antiviral antibody probe can be with the target
Examine thing in viral antigen occur immune response, the viral antigen probe include but is not limited to be and the antiviral antibody pair
The specific antigen answered, the specific antigen include neutralizing one kind in antigen, alexofixagen and blood clotting suppression antigen
Or any combination, the present invention do not do any restrictions to this.Optionally, also include at least on the detecting electrode of the reaction member 3
One layer of closure.The closure is typically prepared using unrelated protein, prevents other materials to be adsorbed onto on detecting electrode to detection
As a result impact, to improve the accuracy of detection.
Optionally, the virus includes but is not limited to be measles virus, poliovirus, Coxsackie virus, prevalence
Property japanese encephalitis virus, dengue virus, hemorrhagic fever viruse, Hantaan virus, Ebola virus, hydrophobin, human immunity lack
Sunken virus, HTLV, hepatitis A virus, hepatitis type B virus, HCV, Hepatitis D virus, penta
Hepatitis virus, herpes simplex virus, varicella virus, cytomegalovirus, EBV, human papilloma virus, colyliform
One kind or any combination in virus, coronavirus and rubella virus.Optionally, if the antiviral antibody includes ebola disease
Poison and two kinds of antibody of hydrophobin, then the antiviral antibody probe then include Ebola virus antigens and Rabies Virus Antigen
Two kinds of antigens, the present invention do not do any restrictions to this.
Specifically, with reference to figure 2, quality control is carried out to the reaction member in above-mentioned antiviral antibody detecting system, can be sieved
Qualified reaction member is selected to be detected to antiviral antibody, the accuracy of antiviral antibody detection is improved, further, by advance
Antiviral antibody probe is coated with, and its quality is controlled, the time of detection antiviral antibody is shortened, to the specialty of operating personnel
Technical requirements also accordingly reduce.
Specifically, the method for quality control is carried out to the reaction member in above-mentioned antiviral antibody detecting system to be included：
Step S1：Antiviral antibody probe is coated on the detecting electrode of a reaction member to be detected；
Step S2：First liquid is placed in the reaction member, first liquid includes deionized water, ultra-pure water, PBS
Any of solution, PBS-T solution and Buffer B；
Step S3：One end of the reaction member to be detected is applied to 40Hz-110MHz pumping signal, is treated described
The size of current of the other end measurement response signal of detection reaction member, and the phase place change relative to pumping signal, and with
This obtains the data message of reaction member to be detected；
Optionally, the data message includes impedance, phase shifting capability, electrical conductivity, resistive component, inductive component and electric capacity
One in component or any combination；The present invention does not do any restrictions to this；
Step S4：The data message of a qualified reaction member is obtained by step S1-S3, contrasts the qualified reaction member
Data message it is whether consistent with the data message of the reaction member to be detected, if unanimously, the reaction member to be detected
It is qualified.
Optionally, in one embodiment of the present invention, when the qualified reaction member data message with it is described to be detected
When the data message of reaction member is inconsistent, reuses antiviral antibody probe solution and be coated with the reaction member, the virus
Antibody probe solution includes specific antigen solution corresponding with the antiviral antibody, and the specific antigen solution includes neutralizing
Antigenic solution, alexofixagen solution and blood clotting suppress antigenic solution, and the present invention is not intended to be limited in any to this；
First liquid is placed in the reaction member to be detected；
One end of the reaction member to be detected is applied to 40Hz-110MHz pumping signal, described to be detected anti-
Answer the other end of unit to measure the size of current of response signal, and the phase place change relative to pumping signal, and obtained with this
The data message of reaction member to be detected, the data message include impedance, electrical conductivity, resistive component, inductive component and electricity
Hold one in component or any combination；
The data message of a qualified reaction member is obtained using above-mentioned steps, contrasts the data letter of the qualified reaction member
Whether breath is consistent with the data message of the reaction member to be detected, if unanimously, the reaction member to be detected is qualified；If no
Unanimously, repeat the above steps, until the reaction member to be detected is qualified.
Optionally, in one embodiment of the present invention, with reference to figure 3, reuse described in the antiviral antibody probe coating
Reaction member to be detected comprises the following steps.
Step Sa：First liquid is placed in reaction member to be detected, 40Hz- is pressed with 50mV-10000mV sine voltage
4MHz does the scanning of frequency electrical conductivity, recording responses electric current and phase difference to each reaction member and selects several of scanning result
Value, the characteristic parameter CS as the reaction member to be detected0；
Step Sb：The first liquid is poured out, the antiviral antibody probe solution of the first concentration is placed in reaction member to be detected
Portion, by 0-120 hours at 20-30 degrees Celsius, relative humidity is to be placed in 70-90 space, the antiviral antibody probe solution
Including specific antigen solution corresponding with the antiviral antibody, the specific antigen solution includes neutralizing antigenic solution, mended
Body combination antigenic solution and blood clotting suppress antigenic solution；Optionally, the antiviral antibody probe for placing 0 hour as the first concentration is molten
The moment that liquid is placed in inside reaction member to be detected.First concentration is corresponding with the concentration of the target detection thing.
The specific antigen can examine the antiviral antibody in thing that immune response occurs with the target, so as to change
The impedance value of reaction member 3 is stated, in one embodiment of the invention, by the change of the impedance value of the reaction member to be detected to institute
Antiviral antibody is stated to be detected；Optionally, those skilled in the art can be compound by extracting the antibody-antigene of immune response generation
Thing, obtain the target and examine composition and quantity of antiviral antibody in thing etc., further studied.
Step Sc：First liquid is placed in reaction member to be detected, 40Hz- is pressed with 50mV-10000mV sine voltage
4MHz does frequency impedance scanning, recording responses electric current and phase difference to reaction member and selects several values of scanning result, makees
For the characteristic parameter CS of the reaction member to be detected1；
Step Sd：The first liquid is poured out, confining liquid is loaded in reaction member to be detected, is taken the photograph by 0-120 hours in 20-30
Family name's degree, relative humidity are to be placed in 70-100 space；
Step Se：First liquid is placed in reaction member to be detected, 40Hz- is pressed with 50mV-10000mV sine voltage
4MHz does frequency impedance scanning, recording responses electric current and phase difference to reaction member and selects several values of scanning result, makees
For the characteristic parameter CS of the reaction member to be detected2；
Step Sf：The first liquid is poured out, examines thing to be put into the target of known the first concentration for including antiviral antibody and treats
Detect in reaction member, perform inspection, obtain three coating parameter CS respectively0、CS1、CS2As reaction member under the concentration
Parameter is coated with, obtains standing time, temperature and humidity corresponding to each parameter.
Optionally, step Sg is also included after Sb steps：To the to be checked of the antiviral antibody probe solution containing the first concentration
Survey the pumping signal that reaction member applies 10Hz~10MHz.It is easy to be internally formed significant thermograde in reaction member and draw
The local fluid flow risen, coating speed of the increase antiviral antibody probe solution on detecting electrode, the present invention do not appoint to this
What is limited.
Optionally, in one embodiment of the invention, it is single to reuse the antiviral antibody probe coating reaction to be detected
The step of member also includes：
Obtaining characteristic parameter CS0Afterwards, cleaning liquid is placed in the reaction member to be detected, to described to be detected
Reaction member is cleaned, and the cleaning liquid includes any of deionized water, ultra-pure water, buffer solution and running water；
Obtaining characteristic parameter CS1Afterwards, cleaning liquid is placed in the reaction member to be detected, to described to be detected
Reaction member is cleaned；And
Obtaining characteristic parameter CS2Afterwards, cleaning liquid is placed in the reaction member to be detected, to described to be detected
Reaction member is cleaned.
Reaction member is cleaned by cleaning liquid, other particulate matters or ion in reaction member is filtered, can increase
Add coating precision, so as to improve the accuracy of detection of reaction member.
The target for being applied to the first concentration by filtering out examines the reaction member of thing, and the target of first concentration is examined
The antiviral antibody tested in thing is detected, and is on the one hand coated with antiviral antibody probe to reaction member in advance, is reduced detection time,
On the other hand, the qualified reaction member selected by step Sa- steps Sf, can obtain corresponding standing time, temperature and
Humidity, increase the accuracy of detection, meanwhile, testing process is simple, the professional technique of operating personnel is required to reduce, so as to reduce
Testing cost, has a feasibility.
In summary, the present invention proposes a kind of antiviral antibody detecting system, by being coated with one in advance in the reaction cell
Antiviral antibody probe with to target examine thing in antiviral antibody detect so that detection time is shorter, and accuracy of detection is higher,
And the professional technique of operating personnel is required to reduce, it is simple to operate；Further, target inspection thing is placed in the reaction member
In, it is increased with the antiviral antibody probe contact area, add detection accuracy；Measured by measuring unit described anti-
The antiviral antibody data message in unit to be answered, and data message is stored to memory cell, testing result is easy to achieve, and
It is easy to those skilled in the art to analyze the testing result of storage, reduces testing cost.
The preferred embodiments of the present invention are above are only, any restrictions effect is not played to the present invention.Belonging to any
Those skilled in the art, in the range of technical scheme is not departed from, to the invention discloses technical scheme and
Technology contents make the variation such as any type of equivalent substitution or modification, belong to the content without departing from technical scheme, still
Belong within protection scope of the present invention.
Claims (9)
- A kind of 1. antiviral antibody detecting system, for detecting the antiviral antibody in target inspection thing, it is characterised in that including reaction Unit, measuring unit and memory cell；The reaction member is coated with an antiviral antibody probe, for being opposite to the reaction Target in unit examines the antiviral antibody in thing to be detected, and the measuring unit is used to measure the disease in the reaction member Malicious Antibody Data information, and stored to the memory cell.
- 2. antiviral antibody detecting system as claimed in claim 1, it is characterised in that the reaction member includes a reaction chamber, The antiviral antibody probe is coated on a detecting electrode of the bottom of the reaction chamber, and the detecting electrode and the measurement are single Member connection.
- 3. antiviral antibody detecting system as claimed in claim 2, it is characterised in that the antiviral antibody probe include with it is described Specific antigen corresponding to antiviral antibody, the specific antigen include neutralization antigen, alexofixagen and blood clotting and suppress anti- It is former.
- 4. antiviral antibody detecting system as claimed in claim 2, it is characterised in that on the detecting electrode of the reaction member also Including at least one layer of closure.
- 5. a kind of method that any reaction member to as described in claim 1-4 carries out quality control, it is characterised in that bag Include：Antiviral antibody probe is coated on the detecting electrode of a reaction member to be detected；First liquid is placed in the reaction member, first liquid includes deionized water, ultra-pure water, PBS solution, PBS-T Any of solution and Buffer B；One end of the reaction member to be detected is applied to 40Hz-110MHz pumping signal, it is single in the reaction to be detected The size of current of the other end measurement response signal of member, and the phase place change relative to pumping signal, and obtained with this to be checked Survey reaction member data message, the data message include impedance, phase shifting capability, electrical conductivity, resistive component, inductive component with And one in capacitive component or any combination；AndThe data message of a qualified reaction member is obtained using above-mentioned steps, contrast the data message of the qualified reaction member with Whether the data message of the reaction member to be detected is consistent, if unanimously, the reaction member to be detected is qualified.
- 6. method of quality control as claimed in claim 5, it is characterised in that also include：When the data message of the qualified reaction member and the inconsistent data message of the reaction member to be detected, make again The reaction member is coated with the antiviral antibody probe, the antiviral antibody probe includes corresponding with the antiviral antibody special Specific Antigen, the specific antigen include neutralizing antigen, alexofixagen and blood clotting suppression antigen；First liquid is placed in the reaction member to be detected；One end of the reaction member to be detected is applied to 40Hz-110MHz pumping signal, it is single in the reaction to be detected The size of current of the other end measurement response signal of member, and the phase place change relative to pumping signal, and obtained with this to be checked The data message of reaction member is surveyed, the data message includes impedance, electrical conductivity, resistive component, inductive component and electric capacity point One in amount or any combination；AndThe data message of a qualified reaction member is obtained using above-mentioned steps, contrast the data message of the qualified reaction member with Whether the data message of the reaction member to be detected is consistent, if unanimously, the reaction member to be detected is qualified；If differ Cause, repeat the above steps, until the reaction member to be detected is qualified.
- 7. method of quality control as claimed in claim 6, it is characterised in that reuse the antiviral antibody probe coating institute The step of stating reaction member to be detected includes：First liquid is placed in reaction member to be detected, with 50mV-10000mV sine voltage by 40Hz-4MHz to each Reaction member does the scanning of frequency electrical conductivity, recording responses electric current and phase difference and selects several values in scanning result, as The characteristic parameter CS of the reaction member to be detected0；The first liquid is poured out, the antiviral antibody probe solution of the first concentration is placed in inside reaction member to be detected, it is small by 0-120 When at 10-40 degrees Celsius, relative humidity is to be placed in 70-90 space, the antiviral antibody probe solution including and the disease Specific antigen solution corresponding to malicious antibody, it is molten that the specific antigen solution includes neutralization antigenic solution, alexofixagen Liquid and blood clotting suppress antigenic solution；First concentration is corresponding with the concentration of the target detection thing；First liquid is placed in reaction member to be detected, with 50mV-10000mV sine voltage by 40Hz-4MHz to reaction Unit does frequency impedance scanning, recording responses electric current and phase difference and selects several values in scanning result, as the reaction The characteristic parameter CS of unit1；The first liquid is poured out, confining liquid is loaded in reaction member to be detected, it is relatively wet by 0-120 hours at 20-30 degrees Celsius Spend in the space for 70-100 and place；First liquid is placed in reaction member to be detected, with 50mV-10000mV sine voltage by 40Hz-4MHz to reaction Unit does frequency impedance scanning, recording responses electric current and phase difference and selects several values in scanning result, as the reaction The characteristic parameter CS of unit2；AndThe first liquid is poured out, examines thing to be put into reaction member to be detected the target of known the first concentration for including antiviral antibody It is interior, inspection is performed, obtains three coating parameter CS respectively0、CS1、CS2Coating ginseng as the reaction member under the first concentration Number, obtains standing time, temperature and humidity corresponding to each parameter.
- 8. method of quality control as claimed in claim 7, it is characterised in that the first liquid is poured out, by the virus of the first concentration Antibody probe solution is placed in inside the reaction member to be detected, by 0-120 hours at 20-30 degrees Celsius, relative humidity 70- Also include after the step of being placed in 90 space：10Hz~10MHz excitation letter is applied to the reaction member to be detected of the antiviral antibody probe solution containing the first concentration Number.
- 9. method of quality control as claimed in claim 7, it is characterised in that reuse the antiviral antibody probe coating institute The step of stating reaction member to be detected also includes：Obtaining characteristic parameter CS0Afterwards, cleaning liquid is placed in the reaction member to be detected, to the reaction to be detected Unit is cleaned, and the cleaning liquid includes any of deionized water, ultra-pure water, buffer solution and running water；Obtaining characteristic parameter CS1Afterwards, cleaning liquid is placed in the reaction member to be detected, to the reaction to be detected Unit is cleaned；AndObtaining characteristic parameter CS2Afterwards, cleaning liquid is placed in the reaction member to be detected, to the reaction to be detected Unit is cleaned.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101166834A (en) * | 2005-04-30 | 2008-04-23 | 柳在泉 | Bio-disc, bio-driver apparatus, and assay method using the same |
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| CN101166834A (en) * | 2005-04-30 | 2008-04-23 | 柳在泉 | Bio-disc, bio-driver apparatus, and assay method using the same |
| CN101315380A (en) * | 2008-07-14 | 2008-12-03 | 湖南大学 | Immune sensor for detecting picloram in compost, and production and use method thereof |
| CN104931692A (en) * | 2015-06-04 | 2015-09-23 | 塔里木大学 | Electrochemical immunosensor for chlorpyrifos detection, and preparation method and application thereof |
| CN106324057A (en) * | 2016-08-01 | 2017-01-11 | 红河学院 | Method for detecting aflatoxin through immunosensor |
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