CN107367541A - A kind of microorganism detection system and detection method of quality control - Google Patents
A kind of microorganism detection system and detection method of quality control Download PDFInfo
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- CN107367541A CN107367541A CN201710643993.8A CN201710643993A CN107367541A CN 107367541 A CN107367541 A CN 107367541A CN 201710643993 A CN201710643993 A CN 201710643993A CN 107367541 A CN107367541 A CN 107367541A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/48—Systems using polarography, i.e. measuring changes in current under a slowly-varying voltage
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
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Abstract
The present invention proposes a kind of microorganism detection system and detection method of quality control, the microorganism in thing is examined to detect target by a microorganism probe, so that detection time is shorter, accuracy of detection is higher, it is simple to operate, and it is easy to those skilled in the art to analyze the testing result of storage, reduce testing cost；Further, quality control is carried out to the reaction member for being coated with microorganism probe in mentioned microorganism detecting system, qualified reaction member can be filtered out with to microorganism detection, the accuracy of microorganism detection is improved, further, by being coated with reaction member in advance, and its quality is controlled, the time of detection microorganism is shortened, improves the sensitivity of detection, the professional technique requirement to operating personnel is also corresponding to be reduced.
Description
Technical field
The present invention relates to bioscience research field, more particularly to a kind of system for detecting microorganism and detection quality control
Method.
Background technology
The detection of microorganism is significant to clinical medicine, and the disease of infectious diseases is studied by the detection of microorganism
Substance feature, improve treatment level；Etiological diagnosis is provided for clinic by accurate microorganism detection, it is proper to be carried out to patient
Work as diagnosis and treatment；Infection can be monitored in time by microorganism detection, instruct reasonable application of antibiotics.
Meanwhile by determining microbiological indicator, judge raw-food material or processing after food whether microbial contamination, be
Food product environment administration of health and food production management and the epidemic prevention of some infectious diseases provide scientific basis.Therefore, microorganism is examined
Measuring tool is significant.
Because target examines the influence of the concentration and surrounding enviroment of thing, micro- life is carried out using existing microorganism detection system
The detection time length of analyte detection, complex operation, and need manual record result, and the professional technique requirement to operating personnel compared with
Height, so as to cause, detection speed is slow and testing cost is high.
Based on this, the present invention proposes a kind of system and method for quality control for detecting microorganism, simple to operate, detection essence
Degree is higher, and has carried out quality control to reaction member in the system of the detection microorganism, screens and qualified is coated with micro- life
The reaction member of physical prospecting pin, improve the accuracy of detection.
The content of the invention
It is an object of the invention to provide detection microorganism system and detection method of quality control so that detection time compared with
Short, accuracy of detection is higher, and simple to operate, requires relatively low to the professional technique of operating personnel.
In order to achieve the above object, one aspect of the present invention provides a kind of microorganism detection system, for detecting target inspection
The microorganism tested in thing, it is characterised in that including reaction member, measuring unit and memory cell；The reaction member coating
There is a microorganism probe, the target for being opposite in the reaction member examines the microorganism in thing to be detected, the survey
Amount unit is used to measure the microbiological data information in the reaction member, and is stored to the memory cell.
Optionally, in mentioned microorganism detecting system, the reaction member includes a reaction chamber, the microorganism probe
It is coated on a detecting electrode of the bottom of the reaction chamber, the detecting electrode is connected with the measuring unit.
Optionally, in mentioned microorganism detecting system, the microorganism probe includes and bacterium, virus, fungi, unwrapping wire
Bacterium, Richettsia, mycoplasma, one kind or any combination of Chlamydia and the corresponding antibody of leptospira antigen.
Optionally, in mentioned microorganism detecting system, at least one layer is also included on the detecting electrode of the reaction member
Closure.
In order to achieve the above object, another aspect of the invention provides a kind of to the progress quality control of any of the above-described reaction member
The method of system, it is characterised in that including：
Microorganism probe is coated on the detecting electrode of a reaction member to be detected；
First liquid is placed in the reaction member, first liquid include deionized water, ultra-pure water, PBS solution,
Any of PBS-T solution and Buffer B；
One end of the reaction member to be detected is applied to 40Hz-110MHz pumping signal, described to be detected anti-
Answer the other end of unit to measure the size of current of response signal, and the phase place change relative to pumping signal, and obtained with this
The data message of reaction member to be detected, the data message include impedance, phase shifting capability, electrical conductivity, resistive component, inductance point
One in amount and capacitive component or any combination；And
The data message of a qualified reaction member is obtained using above-mentioned steps, contrasts the data letter of the qualified reaction member
Whether breath is consistent with the data message of the reaction member to be detected, if unanimously, the reaction member to be detected is qualified.
Optionally, in above-mentioned reaction member method of quality control, in addition to：
When the data message of the qualified reaction member and the inconsistent data message of the reaction member to be detected, weight
The reaction member newly is coated with using the microorganism probe, the microorganism probe includes and bacterium, virus, fungi, unwrapping wire
Bacterium, Richettsia, mycoplasma, one kind or any combination of Chlamydia and the corresponding antibody of leptospira antigen；
First liquid is placed in the reaction member to be detected；
One end of the reaction member to be detected is applied to 40Hz-110MHz pumping signal, described to be detected anti-
Answer the other end of unit to measure the size of current of response signal, and the phase place change relative to pumping signal, and obtained with this
The data message of reaction member to be detected, the data message include impedance, electrical conductivity, resistive component, inductive component and electricity
Hold one in component or any combination；And
The data message of a qualified reaction member is obtained using above-mentioned steps, contrasts the data letter of the qualified reaction member
Whether breath is consistent with the data message of the reaction member to be detected, if unanimously, the reaction member to be detected is qualified；If no
Unanimously, repeat the above steps, until the reaction member to be detected is qualified.
Optionally, in above-mentioned reaction member method of quality control, it is described anti-to reuse the microorganism probe coating
The step of answering unit includes：
First liquid is placed in reaction member to be detected, 40Hz-4MHz pairs is pressed with 50mV-10000mV sine voltage
Each reaction member does the scanning of frequency electrical conductivity, recording responses electric current and phase difference and selects several values in scanning result,
Characteristic parameter CS as the reaction member to be detected0；
The first liquid is poured out, the microorganism probe solution of the first concentration is placed in inside reaction member to be detected, by 0-120
Hour, relative humidity was to be placed in 70-90 space, and the microorganism probe solution includes and bacterium, disease at 10-40 degrees Celsius
Poison, fungi, actinomyces, Richettsia, mycoplasma, Chlamydia and the corresponding antibody of leptospira antigen solution one kind or
Any combination；First concentration is corresponding with the concentration of the target detection thing；
First liquid is placed in reaction member to be detected, 40Hz-4MHz pairs is pressed with 50mV-10000mV sine voltage
Reaction member does frequency impedance scanning, recording responses electric current and phase difference and selects several values in scanning result, is used as this
The characteristic parameter CS of reaction member1；
The first liquid is poured out, confining liquid is loaded in reaction member to be detected, by 0-120 hours at 20-30 degrees Celsius, phase
To humidity be 70-100 space in place；
First liquid is placed in reaction member to be detected, 40Hz-4MHz pairs is pressed with 50mV-10000mV sine voltage
Reaction member does frequency impedance scanning, recording responses electric current and phase difference and selects several values in scanning result, is used as this
The characteristic parameter CS of reaction member2；And
The first liquid is poured out, examines thing to be put into reaction to be detected the target of known the first concentration for including microorganism single
In member, inspection is performed, obtains three coating parameter CS respectively0、CS1、CS2Coating as the reaction member under the first concentration
Parameter, obtain standing time, temperature and humidity corresponding to each parameter.
Optionally, in above-mentioned reaction member method of quality control, the first liquid is poured out, the microorganism of the first concentration is visited
Pin solution is placed in inside the reaction member to be detected, and by 0-120 hours at 20-30 degrees Celsius, relative humidity is 70-90 sky
Also include after the step of interior placement：
10Hz~10MHz excitation letter is applied to the reaction member to be detected of the microorganism probe solution containing the first concentration
Number.
Optionally, in above-mentioned reaction member method of quality control, reuse and treated described in the microorganism probe coating
The step of detecting reaction member also includes：
Obtaining characteristic parameter CS0Afterwards, cleaning liquid is placed in the reaction member to be detected, to described to be detected
Reaction member is cleaned, and the cleaning liquid includes any of deionized water, ultra-pure water, buffer solution and running water；
Obtaining characteristic parameter CS1Afterwards, cleaning liquid is placed in the reaction member to be detected, to described to be detected
Reaction member is cleaned；And
Obtaining characteristic parameter CS2Afterwards, cleaning liquid is placed in the reaction member to be detected, to described to be detected
Reaction member is cleaned.
In summary, the present invention proposes a kind of microorganism detection system, by the way that coating one is micro- in advance in the reaction cell
Bioprobe with to target examine thing in microorganism detect so that detection time is shorter, and accuracy of detection is higher, and to behaviour
The professional technique for making personnel requires to reduce, simple to operate；Further, target inspection thing is placed in the reaction member, makes it
Increase with the microorganism probe contact area, add detection accuracy；Measured by measuring unit in the reaction member
Microbiological data information, and data message is stored to memory cell, testing result is easy to achieve, and is easy to this area
Technical staff is analyzed the testing result of storage, reduces testing cost.
Brief description of the drawings
Fig. 1 is the microorganism detection system structure diagram in one embodiment of the present invention；
Fig. 2 is the flow chart for the method that quality control is carried out to the reaction member of embodiment in Fig. 1；
Fig. 3 is the method flow diagram that coating microorganism probe is re-started when the reaction member of embodiment in Fig. 2 is unqualified.
Specifically, 1- measuring units；11- signaling modules；12 detection modules；2- memory cell；3- reaction members.
Embodiment
The embodiment of the present invention is described in more detail below in conjunction with schematic diagram.According to description below and
Claims, advantages and features of the invention will become apparent from.It should be noted that accompanying drawing is using very simplified form and
Using non-accurately ratio, only for the purpose of facilitating and clarifying the purpose of the embodiments of the invention.
The present invention proposes a kind of microorganism detection system, for detecting the microorganism in target inspection thing, with reference to figure 1, bag
Include reaction member 3, measuring unit 1 and memory cell 2；The reaction member 3 is coated with a microorganism probe, for being opposite to
Target in the reaction member 3 examines the microorganism in thing to be detected, and the measuring unit 1 is used to measure the reaction list
Microbiological data information in member 3, and stored to the memory cell 2.
Preferably, the reaction member 3 in the present embodiment includes a reaction chamber, and the microorganism probe is coated in the reaction
On one detecting electrode of the bottom of chamber, the detecting electrode is connected with the measuring unit 1.In detection process, target examines thing
Reacted with the microorganism probe in the reaction chamber of reaction member 3, micro- life that measuring unit 1 obtains to the reaction
Thing data message measures, and is stored into the memory cell 2.
Optionally, the data message of the microorganism in the present invention includes but is not limited to the impedance value change of the reaction member
Change, the impedance value of the reaction member is transmitted to a memory cell 2 and stored by detection module 12.Microorganism is visited with microorganism
Pin reaction changes the thickness of the detecting electrode of the reaction member, and therefore, the impedance value of reaction member can change, preferably
, in one embodiment of the invention, the change in impedance value of reaction member 3 is detected, the impedance value of the reaction member 3 is transmitted to one
Memory cell 2 is stored.
Optionally, those skilled in the art can analyze and obtain according to the change in impedance value of the reaction member 3 in the present embodiment
Testing result, the testing result include but is not limited to examine microbe species, quantity and the positive reason in thing for the target
Condition etc.；
Composition and species of the microorganism etc., the present invention are obtained according to the analysis to the microbial antigen antibody response
Any restrictions are not done to this.
Specifically, the reaction member is coated with microorganism probe, the microorganism probe includes and bacterium, virus, true
Bacterium, actinomyces, Richettsia, mycoplasma, one kind or any combination of Chlamydia and the corresponding antibody of leptospira antigen, this
Any restrictions are not done in invention to this.For example, detected to the mycoplasma in a target detection thing, then corresponding microorganism probe
For antibody corresponding to the mycoplasma antigen.
Optionally, at least one layer of closure is also included on the detecting electrode of the reaction member 3.The closure is typically adopted
Prepared with unrelated protein, prevent other materials to be adsorbed onto on detecting electrode and testing result is impacted, to improve the standard of detection
True property.
Specifically, with reference to figure 2, quality control is carried out to the reaction member in mentioned microorganism detecting system, can be screened
Go out qualified reaction member to microorganism detection, to improve the accuracy of microorganism detection, it is further, micro- by being coated with advance
Bioprobe, and its quality is controlled, the time of detection microorganism is shortened, the professional technique requirement to operating personnel
It is corresponding to reduce.
Specifically, the method for quality control is carried out to the reaction member in mentioned microorganism detecting system to be included：
Step S1：Microorganism probe is coated on the detecting electrode of a reaction member to be detected；
Step S2：First liquid is placed in the reaction member, first liquid includes deionized water, ultra-pure water, PBS
Any of solution, PBS-T solution and Buffer B；
Step S3：One end of the reaction member to be detected is applied to 40Hz-110MHz pumping signal, is treated described
The size of current of the other end measurement response signal of detection reaction member, and the phase place change relative to pumping signal, and with
This obtains the data message of reaction member to be detected；
Optionally, the data message includes impedance, phase shifting capability, electrical conductivity, resistive component, inductive component and electric capacity
One in component or any combination；The present invention does not do any restrictions to this；
Step S4：The data message of a qualified reaction member is obtained by step S1-S3, contrasts the qualified reaction member
Data message it is whether consistent with the data message of the reaction member to be detected, if unanimously, the reaction member to be detected
It is qualified.
Optionally, in one embodiment of the present invention, when the qualified reaction member data message with it is described to be detected
When the data message of reaction member is inconsistent, reuses microorganism probe solution and be coated with the reaction member, the microorganism
Solution includes corresponding with bacterium, virus, fungi, actinomyces, Richettsia, mycoplasma, Chlamydia and leptospira antigen
One kind or any combination of antibody-solutions；
The present invention is not intended to be limited in any to this；
First liquid is placed in the reaction member to be detected；
One end of the reaction member to be detected is applied to 40Hz-110MHz pumping signal, described to be detected anti-
Answer the other end of unit to measure the size of current of response signal, and the phase place change relative to pumping signal, and obtained with this
The data message of reaction member to be detected, the data message include impedance, electrical conductivity, resistive component, inductive component and electricity
Hold one in component or any combination；
The data message of a qualified reaction member is obtained using above-mentioned steps, contrasts the data letter of the qualified reaction member
Whether breath is consistent with the data message of the reaction member to be detected, if unanimously, the reaction member to be detected is qualified；If no
Unanimously, repeat the above steps, until the reaction member to be detected is qualified.
Optionally, in one embodiment of the present invention, with reference to figure 3, reuse and treated described in the microorganism probe coating
Detection reaction member comprises the following steps.
Step Sa：First liquid is placed in reaction member to be detected, 40Hz- is pressed with 50mV-10000mV sine voltage
4MHz does the scanning of frequency electrical conductivity, recording responses electric current and phase difference to each reaction member and selects several of scanning result
Value, the characteristic parameter CS as the reaction member to be detected0；
Step Sb：The first liquid is poured out, the microorganism probe solution of the first concentration is placed in inside reaction member to be detected,
By 0-120 hours at 20-30 degrees Celsius, relative humidity is to be placed in 70-90 space, the microbial solution including with it is thin
Bacterium, virus, fungi, actinomyces, Richettsia, mycoplasma, the one of Chlamydia and the corresponding antibody-solutions of leptospira antigen
Kind or any combination；Optionally, the microorganism probe solution for placing 0 hour as the first concentration is placed in reaction member to be detected
The moment in portion.First concentration is corresponding with the concentration of the target detection thing.
In one embodiment of the invention, the microorganism is carried out by the change of the impedance value of the reaction member to be detected
Detection；Optionally, those skilled in the art can obtain the microorganism according to the analysis to the microbial antigen antibody response
Composition and species etc., the present invention do not do any restrictions to this.
Step Sc：First liquid is placed in reaction member to be detected, 40Hz- is pressed with 50mV-10000mV sine voltage
4MHz does frequency impedance scanning, recording responses electric current and phase difference to reaction member and selects several values of scanning result, makees
For the characteristic parameter CS of the reaction member to be detected1；
Step Sd：The first liquid is poured out, confining liquid is loaded in reaction member to be detected, is taken the photograph by 0-120 hours in 20-30
Family name's degree, relative humidity are to be placed in 70-100 space；
Step Se：First liquid is placed in reaction member to be detected, 40Hz- is pressed with 50mV-10000mV sine voltage
4MHz does frequency impedance scanning, recording responses electric current and phase difference to reaction member and selects several values of scanning result, makees
For the characteristic parameter CS of the reaction member to be detected2；
Step Sf：The first liquid is poured out, examines thing to be put into the target of known the first concentration for including microorganism to be checked
Survey in reaction member, perform inspection, obtain three coating parameter CS respectively0、CS1、CS2Bag as reaction member under the concentration
By parameter, standing time, temperature and humidity corresponding to each parameter are obtained.
Optionally, step Sg is also included after Sb steps：To the to be detected of the microorganism probe solution containing the first concentration
Reaction member applies 10Hz~10MHz pumping signal.It is easy to be internally formed significant thermograde in reaction member and cause
Local fluid flow, coating speed of the increase microorganism probe solution on detecting electrode, the present invention do not do any limit to this
System.
Optionally, in one embodiment of the invention, reuse the microorganism probe and be coated with the reaction member to be detected
The step of also include：
Obtaining characteristic parameter CS0Afterwards, cleaning liquid is placed in the reaction member to be detected, to described to be detected
Reaction member is cleaned, and the cleaning liquid includes any of deionized water, ultra-pure water, buffer solution and running water；
Obtaining characteristic parameter CS1Afterwards, cleaning liquid is placed in the reaction member to be detected, to described to be detected
Reaction member is cleaned；And
Obtaining characteristic parameter CS2Afterwards, cleaning liquid is placed in the reaction member to be detected, to described to be detected
Reaction member is cleaned.
Reaction member is cleaned by cleaning liquid, other particulate matters or ion in reaction member is filtered, can increase
Add coating precision, so as to improve the accuracy of detection of reaction member.
The target for being applied to the first concentration by filtering out examines the reaction member of thing, and the target of first concentration is examined
The microorganism tested in thing is detected, and is on the one hand coated with microorganism probe to reaction member in advance, is reduced detection time, another
Aspect, the qualified reaction member selected by step Sa- steps Sf, can obtain corresponding standing time, temperature and humidity,
Increase the accuracy of detection, meanwhile, testing process is simple, the professional technique of operating personnel is required to reduce, so as to reduce inspection
Cost is surveyed, there is feasibility.
In summary, the present invention proposes a kind of microorganism detection system, by the way that coating one is micro- in advance in the reaction cell
Bioprobe with to target examine thing in microorganism detect so that detection time is shorter, and accuracy of detection is higher, and to behaviour
The professional technique for making personnel requires to reduce, simple to operate；Further, target inspection thing is placed in the reaction member, makes it
Increase with the microorganism probe contact area, add detection accuracy；Measured by measuring unit in the reaction member
Microbiological data information, and data message is stored to memory cell, testing result is easy to achieve, and is easy to this area
Technical staff is analyzed the testing result of storage, reduces testing cost.
The preferred embodiments of the present invention are above are only, any restrictions effect is not played to the present invention.Belonging to any
Those skilled in the art, in the range of technical scheme is not departed from, to the invention discloses technical scheme and
Technology contents make the variation such as any type of equivalent substitution or modification, belong to the content without departing from technical scheme, still
Belong within protection scope of the present invention.
Claims (9)
- A kind of 1. microorganism detection system, for detecting the microorganism in target inspection thing, it is characterised in that single including reaction Member, measuring unit and memory cell；The reaction member is coated with a microorganism probe, for being opposite to the reaction member In target examine the microorganism in thing to be detected, the measuring unit is used to measure microbe quantity in the reaction member It is believed that breath, and stored to the memory cell.
- 2. microorganism detection system as claimed in claim 1, it is characterised in that the reaction member includes a reaction chamber, institute On the detecting electrode for stating the bottom that microorganism probe is coated in the reaction chamber, the detecting electrode connects with the measuring unit Connect.
- 3. microorganism detection system as claimed in claim 2, it is characterised in that the microorganism probe includes and bacterium, disease Poison, fungi, actinomyces, Richettsia, mycoplasma, Chlamydia and one kind of the corresponding antibody of leptospira antigen or any group Close.
- 4. microorganism detection system as claimed in claim 2, it is characterised in that also wrapped on the detecting electrode of the reaction member Include at least one layer of closure.
- 5. a kind of method that any reaction member to as described in claim 1-4 carries out quality control, it is characterised in that bag Include：Microorganism probe is coated on the detecting electrode of a reaction member to be detected；First liquid is placed in the reaction member, first liquid includes deionized water, ultra-pure water, PBS solution, PBS-T Any of solution and Buffer B；One end of the reaction member to be detected is applied to 40Hz-110MHz pumping signal, it is single in the reaction to be detected The size of current of the other end measurement response signal of member, and the phase place change relative to pumping signal, and obtained with this to be checked Survey reaction member data message, the data message include impedance, phase shifting capability, electrical conductivity, resistive component, inductive component with And one in capacitive component or any combination；AndThe data message of a qualified reaction member is obtained using above-mentioned steps, contrast the data message of the qualified reaction member with Whether the data message of the reaction member to be detected is consistent, if unanimously, the reaction member to be detected is qualified.
- 6. method of quality control as claimed in claim 5, it is characterised in that also include：When the data message of the qualified reaction member and the inconsistent data message of the reaction member to be detected, make again The reaction member is coated with the microorganism probe, and the microorganism probe includes and bacterium, virus, fungi, actinomyces, vertical Gram time body, mycoplasma, one kind or any combination of Chlamydia and the corresponding antibody of leptospira antigen；First liquid is placed in the reaction member to be detected；One end of the reaction member to be detected is applied to 40Hz-110MHz pumping signal, it is single in the reaction to be detected The size of current of the other end measurement response signal of member, and the phase place change relative to pumping signal, and obtained with this to be checked The data message of reaction member is surveyed, the data message includes impedance, electrical conductivity, resistive component, inductive component and electric capacity point One in amount or any combination；AndThe data message of a qualified reaction member is obtained using above-mentioned steps, contrast the data message of the qualified reaction member with Whether the data message of the reaction member to be detected is consistent, if unanimously, the reaction member to be detected is qualified；If differ Cause, repeat the above steps, until the reaction member to be detected is qualified.
- 7. method of quality control as claimed in claim 6, it is characterised in that reuse described in the microorganism probe coating The step of reaction member to be detected, includes：First liquid is placed in reaction member to be detected, with 50mV-10000mV sine voltage by 40Hz-4MHz to each Reaction member does the scanning of frequency electrical conductivity, recording responses electric current and phase difference and selects several values in scanning result, as The characteristic parameter CS of the reaction member to be detected0；The first liquid is poured out, the microorganism probe solution of the first concentration is placed in inside reaction member to be detected, by 0-120 hours At 10-40 degrees Celsius, relative humidity is to be placed in 70-90 space, the microorganism probe solution including with bacterium, virus, Fungi, actinomyces, Richettsia, mycoplasma, Chlamydia and the corresponding antibody of leptospira antigen solution one kind or appoint One combination；First concentration is corresponding with the concentration of the target detection thing；First liquid is placed in reaction member to be detected, with 50mV-10000mV sine voltage by 40Hz-4MHz to reaction Unit does frequency impedance scanning, recording responses electric current and phase difference and selects several values in scanning result, as the reaction The characteristic parameter CS of unit1；The first liquid is poured out, confining liquid is loaded in reaction member to be detected, it is relatively wet by 0-120 hours at 20-30 degrees Celsius Spend in the space for 70-100 and place；First liquid is placed in reaction member to be detected, with 50mV-10000mV sine voltage by 40Hz-4MHz to reaction Unit does frequency impedance scanning, recording responses electric current and phase difference and selects several values in scanning result, as the reaction The characteristic parameter CS of unit2；AndThe first liquid is poured out, examines thing to be put into reaction member to be detected the target of known the first concentration for including microorganism It is interior, inspection is performed, obtains three coating parameter CS respectively0、CS1、CS2Coating ginseng as the reaction member under the first concentration Number, obtains standing time, temperature and humidity corresponding to each parameter.
- 8. method of quality control as claimed in claim 7, it is characterised in that the first liquid is poured out, by micro- life of the first concentration Thing probe solution is placed in inside the reaction member to be detected, by 0-120 hours at 20-30 degrees Celsius, relative humidity 70-90 Space in place the step of after also include：10Hz~10MHz pumping signal is applied to the reaction member to be detected of the microorganism probe solution containing the first concentration.
- 9. method of quality control as claimed in claim 7, it is characterised in that reuse described in the microorganism probe coating The step of reaction member to be detected, also includes：Obtaining characteristic parameter CS0Afterwards, cleaning liquid is placed in the reaction member to be detected, to the reaction to be detected Unit is cleaned, and the cleaning liquid includes any of deionized water, ultra-pure water, buffer solution and running water；Obtaining characteristic parameter CS1Afterwards, cleaning liquid is placed in the reaction member to be detected, to the reaction to be detected Unit is cleaned；AndObtaining characteristic parameter CS2Afterwards, cleaning liquid is placed in the reaction member to be detected, to the reaction to be detected Unit is cleaned.
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| JP2004085353A (en) * | 2002-08-27 | 2004-03-18 | Toshiba Corp | Tubular bio-detection system |
| DE10309349A1 (en) * | 2003-03-03 | 2004-10-21 | Micronas Holding Gmbh | Apparatus for the analysis of an analyte such as a foodstuff, feces, water or fermenter sample, comprises a semiconductor chip carrier with immobilized receptors specific for particular ligands, and a pH sensor |
| CN101115990A (en) * | 2005-02-10 | 2008-01-30 | 株式会社半导体能源研究所 | Semiconductor device |
| CN106324057A (en) * | 2016-08-01 | 2017-01-11 | 红河学院 | Method for detecting aflatoxin through immunosensor |
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