CN107422118A - A kind of virus antigen detection system and detection method of quality control - Google Patents
A kind of virus antigen detection system and detection method of quality control Download PDFInfo
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- CN107422118A CN107422118A CN201710642067.9A CN201710642067A CN107422118A CN 107422118 A CN107422118 A CN 107422118A CN 201710642067 A CN201710642067 A CN 201710642067A CN 107422118 A CN107422118 A CN 107422118A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
- G01N33/5438—Electrodes
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Abstract
The present invention proposes a kind of virus antigen detection system and detection method of quality control, the viral antigen in thing is examined to detect target by a viral antigen probe, so that detection time is shorter, accuracy of detection is higher, it is simple to operate, and it is easy to those skilled in the art to analyze the testing result of storage, reduce testing cost；Further, quality control is carried out to the reaction member for being coated with viral antigen probe in above-mentioned virus antigen detection system, qualified reaction member can be filtered out with to virus antigen detection, the accuracy of virus antigen detection is improved, further, by being coated with reaction member in advance, and its quality is controlled, the time of detection viral antigen is shortened, improves the sensitivity of detection, the professional technique requirement to operating personnel is also corresponding to be reduced.
Description
Technical field
The present invention relates to biological technical field, more particularly to a kind of system for detecting viral antigen and detection quality control side
Method.
Background technology
Virus is the acellular form being made up of a nucleic acid molecules and protein, by parasitic life between life entity and
Organic species between non-life body, it, which can infect, nearly all has cyto-architectural life entity.
Clinical manifestation caused by the infection of virus can not be distinguished in time in clinical or experimentation, it is therefore desirable to quick inspection
Survey species and the cycle of virus, in order to aid in case control, and take effective control measure, at present, it is more using using Electronic Speculum,
Immuno-electron microscope, immunofluorescence technique, ELISA and radioimmunology etc. can directly check virion and virus in sample
Antigen, it is commonly used for quick and early diagnosis.
Because target examines the influence of the concentration and surrounding enviroment of thing, disease is carried out using existing virus antigen detection method
The detection time length of malicious antigen detection, complex operation, and manual record result is needed, and to the professional technique requirement of operating personnel
Higher, so as to cause, detection speed is slow and testing cost is high.
Based on this, the present invention proposes a kind of system and method for quality control for detecting viral antigen, simple to operate, detection
Precision is higher, and has carried out quality control to reaction member in the system of the detection viral antigen, screens qualified be coated with
The reaction member of viral antigen probe, improve the accuracy of detection.
The content of the invention
It is an object of the invention to provide the system of detection viral antigen and detection method of quality control so that detection time
Shorter, accuracy of detection is higher, and simple to operate, requires relatively low to the professional technique of operating personnel.
In order to achieve the above object, one aspect of the present invention provides a kind of virus antigen detection system, for detecting target
Examine the viral antigen in thing, it is characterised in that including reaction member, measuring unit and memory cell；The reaction member
A viral antigen probe is coated with, the target for being opposite in the reaction member examines the viral antigen in thing to be examined
Survey, the measuring unit is used to measure the viral antigen data message in the reaction member, and is stored to the storage
Unit.
Optionally, in above-mentioned virus antigen detection system, the reaction member includes a reaction chamber, the viral antigen
Probe is coated on a detecting electrode of the bottom of the reaction chamber, and the detecting electrode is connected with the measuring unit.
Optionally, in above-mentioned virus antigen detection system, the viral antigen probe causes including the viral antigen
Specific antibody, the specific antibody includes neutralizing antibody, complement fixation antibody and hemagglutination inhibition antibody.
Optionally, in above-mentioned virus antigen detection system, at least one is also included on the detecting electrode of the reaction member
Layer closure.
In order to achieve the above object, another aspect of the invention provides a kind of to the progress quality control of any of the above-described reaction member
The method of system, it is characterised in that including：
Viral antigen probe is coated on the detecting electrode of a reaction member to be detected；
First liquid is placed in the reaction member, first liquid include deionized water, ultra-pure water, PBS solution,
Any of PBS-T solution and Buffer B；
One end of the reaction member to be detected is applied to 40Hz-110MHz pumping signal, described to be detected anti-
Answer the other end of unit to measure the size of current of response signal, and the phase place change relative to pumping signal, and obtained with this
The data message of reaction member to be detected, the data message include impedance, phase shifting capability, electrical conductivity, resistive component, inductance point
One in amount and capacitive component or any combination；And
The data message of a qualified reaction member is obtained using above-mentioned steps, contrasts the data letter of the qualified reaction member
Whether breath is consistent with the data message of the reaction member to be detected, if unanimously, the reaction member to be detected is qualified.
Optionally, in above-mentioned reaction member method of quality control, in addition to：
When the data message of the qualified reaction member and the inconsistent data message of the reaction member to be detected, weight
The reaction member newly is coated with using the viral antigen probe, the viral antigen probe is included caused by the viral antigen
Specific antibody, the specific antibody include neutralizing antibody, complement fixation antibody and hemagglutination inhibition antibody；
First liquid is placed in the reaction member to be detected；
One end of the reaction member to be detected is applied to 40Hz-110MHz pumping signal, described to be detected anti-
Answer the other end of unit to measure the size of current of response signal, and the phase place change relative to pumping signal, and obtained with this
The data message of reaction member to be detected, the data message include impedance, electrical conductivity, resistive component, inductive component and electricity
Hold one in component or any combination；And
The data message of a qualified reaction member is obtained using above-mentioned steps, contrasts the data letter of the qualified reaction member
Whether breath is consistent with the data message of the reaction member to be detected, if unanimously, the reaction member to be detected is qualified；If no
Unanimously, repeat the above steps, until the reaction member to be detected is qualified.
Optionally, in above-mentioned reaction member method of quality control, reuse described in the viral antigen probe coating
The step of reaction member, includes：
First liquid is placed in reaction member to be detected, 40Hz-4MHz pairs is pressed with 50mV-10000mV sine voltage
Each reaction member does the scanning of frequency electrical conductivity, recording responses electric current and phase difference and selects several values in scanning result,
Characteristic parameter CS as the reaction member to be detected0；
The first liquid is poured out, the viral antigen probe solution of the first concentration is placed in inside reaction member to be detected, by 0-
At 10-40 degrees Celsius, relative humidity is to be placed in 70-90 space, and the viral antigen probe solution includes described within 120 hours
Specific antibody solution caused by viral antigen, the specific antibody solution include neutralizing antibody, complement fixation antibody and blood
It is solidifying to suppress antibody-solutions；First concentration is corresponding with the concentration of the target detection thing；
First liquid is placed in reaction member to be detected, 40Hz-4MHz pairs is pressed with 50mV-10000mV sine voltage
Reaction member does frequency impedance scanning, recording responses electric current and phase difference and selects several values in scanning result, is used as this
The characteristic parameter CS of reaction member1；
The first liquid is poured out, confining liquid is loaded in reaction member to be detected, by 0-120 hours at 20-30 degrees Celsius, phase
To humidity be 70-100 space in place；
First liquid is placed in reaction member to be detected, 40Hz-4MHz pairs is pressed with 50mV-10000mV sine voltage
Reaction member does frequency impedance scanning, recording responses electric current and phase difference and selects several values in scanning result, is used as this
The characteristic parameter CS of reaction member2；And
The first liquid is poured out, examines thing to be put into reaction to be detected the target of known the first concentration for including viral antigen
In unit, inspection is performed, obtains three coating parameter CS respectively0、CS1、CS2Bag as the reaction member under the first concentration
By parameter, standing time, temperature and humidity corresponding to each parameter are obtained.
Optionally, in above-mentioned reaction member method of quality control, the first liquid is poured out, by the viral antigen of the first concentration
Probe solution is placed in inside the reaction member to be detected, and by 0-120 hours at 20-30 degrees Celsius, relative humidity is 70-90's
Also include after the step of being placed in space：
10Hz~10MHz excitation is applied to the reaction member to be detected of the viral antigen probe solution containing the first concentration
Signal.
Optionally, in above-mentioned reaction member method of quality control, reuse described in the viral antigen probe coating
The step of reaction member to be detected, also includes：
Obtaining characteristic parameter CS0Afterwards, cleaning liquid is placed in the reaction member to be detected, to described to be detected
Reaction member is cleaned, and the cleaning liquid includes any of deionized water, ultra-pure water, buffer solution and running water；
Obtaining characteristic parameter CS1Afterwards, cleaning liquid is placed in the reaction member to be detected, to described to be detected
Reaction member is cleaned；And
Obtaining characteristic parameter CS2Afterwards, cleaning liquid is placed in the reaction member to be detected, to described to be detected
Reaction member is cleaned.
In summary, the present invention proposes a kind of virus antigen detection system, by being coated with one in advance in the reaction cell
Viral antigen probe with to target examine thing in viral antigen detect so that detection time is shorter, and accuracy of detection is higher,
And the professional technique of operating personnel is required to reduce, it is simple to operate；Further, target inspection thing is placed in the reaction member
In, it is increased with the viral antigen probe contact area, add detection accuracy；Measured by measuring unit described anti-
The viral antigen data message in unit to be answered, and data message is stored to memory cell, testing result is easy to achieve, and
It is easy to those skilled in the art to analyze the testing result of storage, reduces testing cost.
Brief description of the drawings
Fig. 1 is the virus antigen detection system structure diagram in one embodiment of the present invention；
Fig. 2 is the flow chart for the method that quality control is carried out to the reaction member of embodiment in Fig. 1；
Fig. 3 is the method flow that coating viral antigen probe is re-started when the reaction member of embodiment in Fig. 2 is unqualified
Figure.
Specifically, 1- measuring units；11- signaling modules；12 detection modules；2- memory cell；3- reaction members.
Embodiment
The embodiment of the present invention is described in more detail below in conjunction with schematic diagram.According to description below and
Claims, advantages and features of the invention will become apparent from.It should be noted that accompanying drawing is using very simplified form and
Using non-accurately ratio, only for the purpose of facilitating and clarifying the purpose of the embodiments of the invention.
The present invention proposes a kind of virus antigen detection system, for detecting the viral antigen in target inspection thing, reference chart
1, including reaction member 3, measuring unit 1 and memory cell 2；The reaction member 3 is coated with a viral antigen probe, is used for
The target being opposite in the reaction member 3 examines the viral antigen in thing to be detected, and the measuring unit 1 is used to measure institute
The viral antigen data message in reaction member 3 is stated, and is stored to the memory cell 2.
Preferably, the reaction member 3 in the present embodiment includes a reaction chamber, and the viral antigen probe is coated in described anti-
Answer on a detecting electrode of the bottom of chamber, the detecting electrode is connected with the measuring unit 1.In detection process, target is examined
Thing reacts with the viral antigen probe in the reaction chamber of reaction member 3, and measuring unit 1 obtains to the reaction
Viral antigen data message measures, and is stored into the memory cell 2.
Optionally, the data message of the viral antigen in the present invention includes but is not limited to the impedance value of the reaction member
The impedance value of the reaction member is transmitted to a memory cell 2 and stored by change, detection module 12.Viral antigen with it is described
Immune response occurs for specific antibody caused by viral antigen, changes the thickness of the detecting electrode of the reaction member, therefore,
The impedance value of reaction member can change, it is preferred that in one embodiment of the invention, the change in impedance value of reaction member 3 is detected,
The impedance value of the reaction member 3 is transmitted to a memory cell 2 and stored.
Optionally, those skilled in the art can analyze and obtain according to the change in impedance value of the reaction member 3 in the present embodiment
Testing result, viral antigen species that the testing result includes but is not limited to examine for the target in thing, quantity and normal
Situation etc.；It is right according to composition of viral antigen etc. described in the response analysis to the viral antigen and specific antibody, the present invention
This does not do any restrictions.
Specifically, the reaction member is coated with viral antigen probe, and the viral antigen probe can be with the target
Examine the viral antigen in thing that immune response occurs, the viral antigen probe includes but is not limited to cause for the viral antigen
Specific antibody, the specific antibody includes but is not limited in neutralizing antibody, complement fixation antibody and hemagglutination inhibition antibody
One kind or any combination, the present invention any restrictions are not done to this.Optionally, also wrapped on the detecting electrode of the reaction member 3
Include at least one layer of closure.The closure is typically prepared using unrelated protein, prevents other materials to be adsorbed onto on detecting electrode
Testing result is impacted, to improve the accuracy of detection.
Optionally, it is measles virus that the viral antigen, which includes but is not limited to, poliovirus, Coxsackie virus,
Epidemic encephalitis B virus, dengue virus, hemorrhagic fever viruse, Hantaan virus, Ebola virus, hydrophobin, the mankind exempt from
Epidemic disease defective virus, HTLV, hepatitis A virus, hepatitis type B virus, HCV, hepatitis D disease
Poison, HEV, herpes simplex virus, varicella virus, cytomegalovirus, EBV, human papilloma virus,
One kind or any combination in rotavirus, coronavirus and rubella virus.Optionally, if the viral antigen include it is angstrom rich
Virus and two kinds of antigens of hydrophobin are drawn, then the viral antigen probe then includes Ebola virus antibody and hydrophobin
Two kinds of antibody, the present invention do not do any restrictions to this.
Specifically, with reference to figure 2, quality control is carried out to the reaction member in above-mentioned virus antigen detection system, can be sieved
Qualified reaction member is selected to virus antigen detection, to improve the accuracy of virus antigen detection, further, by advance
Viral antigen probe is coated with, and its quality is controlled, the time of detection viral antigen is shortened, to the specialty of operating personnel
Technical requirements also accordingly reduce.
Specifically, the method for quality control is carried out to the reaction member in above-mentioned virus antigen detection system to be included：
Step S1：Viral antigen probe is coated on the detecting electrode of a reaction member to be detected；
Step S2：First liquid is placed in the reaction member, first liquid includes deionized water, ultra-pure water, PBS
Any of solution, PBS-T solution and Buffer B；
Step S3：One end of the reaction member to be detected is applied to 40Hz-110MHz pumping signal, is treated described
The size of current of the other end measurement response signal of detection reaction member, and the phase place change relative to pumping signal, and with
This obtains the data message of reaction member to be detected；
Optionally, the data message includes impedance, phase shifting capability, electrical conductivity, resistive component, inductive component and electric capacity
One in component or any combination；The present invention does not do any restrictions to this；
Step S4：The data message of a qualified reaction member is obtained by step S1-S3, contrasts the qualified reaction member
Data message it is whether consistent with the data message of the reaction member to be detected, if unanimously, the reaction member to be detected
It is qualified.
Optionally, in one embodiment of the present invention, when the qualified reaction member data message with it is described to be detected
When the data message of reaction member is inconsistent, reuses viral antigen probe solution and be coated with the reaction member, the virus
Antigen probe solution includes specific antibody solution caused by the viral antigen, and the specific antibody solution includes neutralizing and resisted
Body, complement fixation antibody and hemagglutination inhibition antibody solution, the present invention are not intended to be limited in any to this；
First liquid is placed in the reaction member to be detected；
One end of the reaction member to be detected is applied to 40Hz-110MHz pumping signal, described to be detected anti-
Answer the other end of unit to measure the size of current of response signal, and the phase place change relative to pumping signal, and obtained with this
The data message of reaction member to be detected, the data message include impedance, electrical conductivity, resistive component, inductive component and electricity
Hold one in component or any combination；
The data message of a qualified reaction member is obtained using above-mentioned steps, contrasts the data letter of the qualified reaction member
Whether breath is consistent with the data message of the reaction member to be detected, if unanimously, the reaction member to be detected is qualified；If no
Unanimously, repeat the above steps, until the reaction member to be detected is qualified.
Optionally, in one embodiment of the present invention, with reference to figure 3, reuse described in the viral antigen probe coating
Reaction member to be detected comprises the following steps.
Step Sa：First liquid is placed in reaction member to be detected, 40Hz- is pressed with 50mV-10000mV sine voltage
4MHz does the scanning of frequency electrical conductivity, recording responses electric current and phase difference to each reaction member and selects several of scanning result
Value, the characteristic parameter CS as the reaction member to be detected0；
Step Sb：The first liquid is poured out, the viral antigen probe solution of the first concentration is placed in reaction member to be detected
Portion, by 0-120 hours at 20-30 degrees Celsius, relative humidity is to be placed in 70-90 space, the viral antigen probe solution
Including specific antibody solution caused by the viral antigen, the specific antibody solution includes neutralizing antibody, complement combines
Antibody and hemagglutination inhibition antibody solution；Optionally, the viral antigen probe solution of 0 hour as the first concentration of placement is placed in be checked
The moment surveyed inside reaction member.First concentration is corresponding with the concentration of the target detection thing.
The specific antibody can examine the viral antigen in thing that immune response occurs with the target, so as to change
The impedance value of reaction member 3 is stated, in one embodiment of the invention, by the change of the impedance value of the reaction member to be detected to institute
Viral antigen is stated to be detected；Optionally, those skilled in the art can be compound by extracting the antibody-antigene of immune response generation
Thing, obtain the target and examine composition and quantity of viral antigen in thing etc., further studied.
Step Sc：First liquid is placed in reaction member to be detected, 40Hz- is pressed with 50mV-10000mV sine voltage
4MHz does frequency impedance scanning, recording responses electric current and phase difference to reaction member and selects several values of scanning result, makees
For the characteristic parameter CS of the reaction member to be detected1；
Step Sd：The first liquid is poured out, confining liquid is loaded in reaction member to be detected, is taken the photograph by 0-120 hours in 20-30
Family name's degree, relative humidity are to be placed in 70-100 space；
Step Se：First liquid is placed in reaction member to be detected, 40Hz- is pressed with 50mV-10000mV sine voltage
4MHz does frequency impedance scanning, recording responses electric current and phase difference to reaction member and selects several values of scanning result, makees
For the characteristic parameter CS of the reaction member to be detected2；
Step Sf：The first liquid is poured out, examines thing to be put into the target of known the first concentration for including viral antigen and treats
Detect in reaction member, perform inspection, obtain three coating parameter CS respectively0、CS1、CS2As reaction member under the concentration
Parameter is coated with, obtains standing time, temperature and humidity corresponding to each parameter.
Optionally, step Sg is also included after Sb steps：To the to be checked of the viral antigen probe solution containing the first concentration
Survey the pumping signal that reaction member applies 10Hz~10MHz.It is easy to be internally formed significant thermograde in reaction member and draw
The local fluid flow risen, coating speed of the increase viral antigen probe solution on detecting electrode, the present invention do not appoint to this
What is limited.
Optionally, in one embodiment of the invention, it is single to reuse the viral antigen probe coating reaction to be detected
The step of member also includes：
Obtaining characteristic parameter CS0Afterwards, cleaning liquid is placed in the reaction member to be detected, to described to be detected
Reaction member is cleaned, and the cleaning liquid includes any of deionized water, ultra-pure water, buffer solution and running water；
Obtaining characteristic parameter CS1Afterwards, cleaning liquid is placed in the reaction member to be detected, to described to be detected
Reaction member is cleaned；And
Obtaining characteristic parameter CS2Afterwards, cleaning liquid is placed in the reaction member to be detected, to described to be detected
Reaction member is cleaned.
Reaction member is cleaned by cleaning liquid, other particulate matters or ion in reaction member is filtered, can increase
Add coating precision, so as to improve the accuracy of detection of reaction member.
The target for being applied to the first concentration by filtering out examines the reaction member of thing, and the target of first concentration is examined
The viral antigen tested in thing is detected, and is on the one hand coated with viral antigen probe to reaction member in advance, is reduced detection time,
On the other hand, the qualified reaction member selected by step Sa- steps Sf, can obtain corresponding standing time, temperature and
Humidity, increase the accuracy of detection, meanwhile, testing process is simple, the professional technique of operating personnel is required to reduce, so as to reduce
Testing cost, has a feasibility.
In summary, the present invention proposes a kind of virus antigen detection system, by being coated with one in advance in the reaction cell
Viral antigen probe with to target examine thing in viral antigen detect so that detection time is shorter, and accuracy of detection is higher,
And the professional technique of operating personnel is required to reduce, it is simple to operate；Further, target inspection thing is placed in the reaction member
In, it is increased with the viral antigen probe contact area, add detection accuracy；Measured by measuring unit described anti-
The viral antigen data message in unit to be answered, and data message is stored to memory cell, testing result is easy to achieve, and
It is easy to those skilled in the art to analyze the testing result of storage, reduces testing cost.
The preferred embodiments of the present invention are above are only, any restrictions effect is not played to the present invention.Belonging to any
Those skilled in the art, in the range of technical scheme is not departed from, to the invention discloses technical scheme and
Technology contents make the variation such as any type of equivalent substitution or modification, belong to the content without departing from technical scheme, still
Belong within protection scope of the present invention.
Claims (9)
- A kind of 1. virus antigen detection system, for detecting the viral antigen in target inspection thing, it is characterised in that including reaction Unit, measuring unit and memory cell；The reaction member is coated with a viral antigen probe, for being opposite to the reaction Target in unit examines the viral antigen in thing to be detected, and the measuring unit is used to measure the disease in the reaction member Malicious antigen data information, and stored to the memory cell.
- 2. virus antigen detection system as claimed in claim 1, it is characterised in that the reaction member includes a reaction chamber, The viral antigen probe is coated on a detecting electrode of the bottom of the reaction chamber, and the detecting electrode and the measurement are single Member connection.
- 3. virus antigen detection system as claimed in claim 2, it is characterised in that the viral antigen probe includes the disease Specific antibody caused by malicious antigen, the specific antibody include neutralizing antibody, complement fixation antibody and hemagglutination inhibition antibody.
- 4. virus antigen detection system as claimed in claim 2, it is characterised in that on the detecting electrode of the reaction member also Including at least one layer of closure.
- 5. a kind of method that any reaction member to as described in claim 1-4 carries out quality control, it is characterised in that bag Include：Viral antigen probe is coated on the detecting electrode of a reaction member to be detected；First liquid is placed in the reaction member, first liquid includes deionized water, ultra-pure water, PBS solution, PBS-T Any of solution and Buffer B；One end of the reaction member to be detected is applied to 40Hz-110MHz pumping signal, it is single in the reaction to be detected The size of current of the other end measurement response signal of member, and the phase place change relative to pumping signal, and obtained with this to be checked Survey reaction member data message, the data message include impedance, phase shifting capability, electrical conductivity, resistive component, inductive component with And one in capacitive component or any combination；AndThe data message of a qualified reaction member is obtained using above-mentioned steps, contrast the data message of the qualified reaction member with Whether the data message of the reaction member to be detected is consistent, if unanimously, the reaction member to be detected is qualified.
- 6. method of quality control as claimed in claim 5, it is characterised in that also include：When the data message of the qualified reaction member and the inconsistent data message of the reaction member to be detected, make again The reaction member is coated with the viral antigen probe, the viral antigen probe includes special caused by the viral antigen Property antibody, the specific antibody include neutralizing antibody, complement fixation antibody and hemagglutination inhibition antibody；First liquid is placed in the reaction member to be detected；One end of the reaction member to be detected is applied to 40Hz-110MHz pumping signal, it is single in the reaction to be detected The size of current of the other end measurement response signal of member, and the phase place change relative to pumping signal, and obtained with this to be checked The data message of reaction member is surveyed, the data message includes impedance, electrical conductivity, resistive component, inductive component and electric capacity point One in amount or any combination；AndThe data message of a qualified reaction member is obtained using above-mentioned steps, contrast the data message of the qualified reaction member with Whether the data message of the reaction member to be detected is consistent, if unanimously, the reaction member to be detected is qualified；If differ Cause, repeat the above steps, until the reaction member to be detected is qualified.
- 7. method of quality control as claimed in claim 6, it is characterised in that reuse the viral antigen probe coating institute The step of stating reaction member to be detected includes：First liquid is placed in reaction member to be detected, with 50mV-10000mV sine voltage by 40Hz-4MHz to each Reaction member does the scanning of frequency electrical conductivity, recording responses electric current and phase difference and selects several values in scanning result, as The characteristic parameter CS of the reaction member to be detected0；The first liquid is poured out, the viral antigen probe solution of the first concentration is placed in inside reaction member to be detected, it is small by 0-120 When at 10-40 degrees Celsius, relative humidity is that placement, the viral antigen probe solution include the virus in 70-90 space The solution of specific antibody caused by antigen, the specific antibody solution include neutralizing antibody, complement fixation antibody and blood clotting Suppress antibody-solutions；First concentration is corresponding with the concentration of the target detection thing；First liquid is placed in reaction member to be detected, with 50mV-10000mV sine voltage by 40Hz-4MHz to reaction Unit does frequency impedance scanning, recording responses electric current and phase difference and selects several values in scanning result, as the reaction The characteristic parameter CS of unit1；The first liquid is poured out, confining liquid is loaded in reaction member to be detected, it is relatively wet by 0-120 hours at 20-30 degrees Celsius Spend in the space for 70-100 and place；First liquid is placed in reaction member to be detected, with 50mV-10000mV sine voltage by 40Hz-4MHz to reaction Unit does frequency impedance scanning, recording responses electric current and phase difference and selects several values in scanning result, as the reaction The characteristic parameter CS of unit2；AndThe first liquid is poured out, examines thing to be put into reaction member to be detected the target of known the first concentration for including viral antigen It is interior, inspection is performed, obtains three coating parameter CS respectively0、CS1、CS2Coating ginseng as the reaction member under the first concentration Number, obtains standing time, temperature and humidity corresponding to each parameter.
- 8. method of quality control as claimed in claim 7, it is characterised in that the first liquid is poured out, by the virus of the first concentration Antigen probe solution is placed in inside the reaction member to be detected, by 0-120 hours at 20-30 degrees Celsius, relative humidity 70- Also include after the step of being placed in 90 space：10Hz~10MHz excitation letter is applied to the reaction member to be detected of the viral antigen probe solution containing the first concentration Number.
- 9. method of quality control as claimed in claim 7, it is characterised in that reuse the viral antigen probe coating institute The step of stating reaction member to be detected also includes：Obtaining characteristic parameter CS0Afterwards, cleaning liquid is placed in the reaction member to be detected, to the reaction to be detected Unit is cleaned, and the cleaning liquid includes any of deionized water, ultra-pure water, buffer solution and running water；Obtaining characteristic parameter CS1Afterwards, cleaning liquid is placed in the reaction member to be detected, to the reaction to be detected Unit is cleaned；AndObtaining characteristic parameter CS2Afterwards, cleaning liquid is placed in the reaction member to be detected, to the reaction to be detected Unit is cleaned.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710642067.9A CN107422118A (en) | 2017-07-31 | 2017-07-31 | A kind of virus antigen detection system and detection method of quality control |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710642067.9A CN107422118A (en) | 2017-07-31 | 2017-07-31 | A kind of virus antigen detection system and detection method of quality control |
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| Publication Number | Publication Date |
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| CN107422118A true CN107422118A (en) | 2017-12-01 |
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| CN111855776A (en) * | 2020-07-06 | 2020-10-30 | 新视界视光健康产业（南京）有限公司 | Glasses capable of measuring virus |
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| WO2013125855A1 (en) * | 2012-02-20 | 2013-08-29 | 주식회사 나노엔텍 | Novel method for detecting antigen, and apparatus using same |
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| WO2013125855A1 (en) * | 2012-02-20 | 2013-08-29 | 주식회사 나노엔텍 | Novel method for detecting antigen, and apparatus using same |
| CN104965080A (en) * | 2015-05-29 | 2015-10-07 | 重庆大学 | Reaction unit and system for detecting antibody or antigen |
| CN106324057A (en) * | 2016-08-01 | 2017-01-11 | 红河学院 | Method for detecting aflatoxin through immunosensor |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111855776A (en) * | 2020-07-06 | 2020-10-30 | 新视界视光健康产业（南京）有限公司 | Glasses capable of measuring virus |
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