CN107367613A - The detection method and bacterial antigens probe method for coating of a kind of bacterial antigens - Google Patents

The detection method and bacterial antigens probe method for coating of a kind of bacterial antigens Download PDF

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Publication number
CN107367613A
CN107367613A CN201710642999.3A CN201710642999A CN107367613A CN 107367613 A CN107367613 A CN 107367613A CN 201710642999 A CN201710642999 A CN 201710642999A CN 107367613 A CN107367613 A CN 107367613A
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reaction member
bacterial antigens
thing
target
reaction
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刘晓竹
冯钶
童立
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Chongqing Aoyun Micro Biological Technology Co Ltd
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Chongqing Aoyun Micro Biological Technology Co Ltd
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria

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  • Life Sciences & Earth Sciences (AREA)
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  • Biotechnology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
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  • General Health & Medical Sciences (AREA)
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The present invention proposes a kind of method of detection bacterium antigen, target inspection thing is placed in the reaction member for being coated with bacterial antigens probe, the bacterial antigens in thing are examined to detect the target in the reaction member by measuring unit, and detection information is stored and analyzed, obtain the testing result of bacterial antigens, testing result is easy to achieve, and is easy to those skilled in the art to analyze the testing result of storage, reduces testing cost；Further, bacterial antigens probe is coated on reaction member in advance, on the one hand reduces detection time, on the one hand the reaction member after screening coating carries out bacterial antigens detection, improves accuracy of detection and detection accuracy.

Description

The detection method and bacterial antigens probe method for coating of a kind of bacterial antigens
Technical field
The present invention relates to bioscience research field, more particularly to a kind of detection method of bacterial antigens.
Background technology
Bacterium also has a great impact to mankind's activity.On the one hand, bacterium is the pathogen of many diseases, including lung knot The diseases such as core, gonorrhoea, anthracnose, syphilis, the plague, sand holes are triggered by bacterium.However, the mankind also utilize bacterium often, Such as the making of cheese and Yoghourt and fermented glutinous rice, the manufacture of part antibiotic, processing of waste water etc., it is all relevant with bacterium.In biology In sciemtifec and technical sphere, bacterium, which has, also writes extensive use.Therefore, the detection of bacterial antigens is respectively provided with clinical and field of food safety Significance.
Because target examines the influence of the concentration and surrounding enviroment of thing, existing bacterial antigens detection method, detection time It is long, complex operation, and manual record result is needed, and higher is required to the professional technique of operating personnel, so as to cause detection speed Degree is slow and testing cost is high.
Based on this, the present invention proposes a kind of bacterial antigens detection method, and simple to operate, accuracy of detection is higher, and passes through Coating bacterial antigens probe in advance so that detection time is shorter, requires to reduce to the professional technique of operating personnel.
The content of the invention
It is an object of the invention to provide a kind of bacterial antigens detection method so that detection time is shorter, accuracy of detection compared with Height, and it is simple to operate, relatively low is required to the professional technique of operating personnel.
In order to achieve the above object, the invention provides a kind of method of detection bacterium antigen, examined for detecting target Bacterial antigens in thing, it is characterised in that including：
Determine that the target examines thing can concentrations scope；
A reaction member is obtained, the reaction member is coated with bacterial antigens probe in advance；
Target inspection thing is placed in the reaction member, the bacterial antigens probe is examined in thing with the target Bacterial antigens react；
The target is obtained by measuring unit and examines the data message of the bacterial antigens in thing, and is transmitted single to a storage Member is stored；And
The data message in the memory cell is analyzed, obtains testing result.
Optionally, in the method for above-mentioned detection bacterium antigen, the reaction member includes a reaction chamber, and the bacterium resists Former probe is coated on a detecting electrode of the bottom of the reaction chamber, and the detecting electrode is connected with the measuring unit.
Optionally, in the method for above-mentioned detection bacterium antigen, the reaction member is coated with bacterial antigens probe in advance Step includes：
Obtain bacterial antigens probe solution corresponding with target inspection thing concentration；
The coating parameter of the reaction member is obtained, according to the coating parameter of the reaction member, the bacterium is obtained and resists Coating time, temperature and the humidity of former probe solution；And
The bacterial antigens probe solution is placed in the reaction member, according to coating time, temperature and the humidity The reaction member is coated with；Also include at least one layer of closure on the detecting electrode of the reaction member.
Optionally, wrapped in the method for above-mentioned detection bacterium antigen, the step of the coating parameter for obtaining the reaction member Include：
First liquid is placed in the reaction member, first liquid includes deionized water, ultra-pure water, buffer solution, PBS Any of solution, Buffer B and running water, the salt content of the ultra-pure water are less than 0.3mg/L, and electrical conductivity is less than 0.2 μs/cm；
Make the scanning of frequency electrical conductivity to the reaction member by 40Hz-4MHz of 50mV-10000mV sine voltage, Recording responses electric current and phase difference simultaneously select several values in scanning result, the characteristic parameter CS as the reaction member0；
The first liquid is poured out, the bacterial antigens probe solution of various concentrations is placed in the reaction member, by 0-120 hours At 20-30 degrees Celsius, relative humidity is to be placed in 70-90 space, and the bacterial antigens probe solution includes and the bacterium Bacterial antigens antibody-solutions corresponding to antigen；
First liquid is placed in the reaction member, with 50mV-10000mV sine voltage by 40Hz-4MHz to anti- Answer unit to do frequency impedance scanning, recording responses electric current and phase difference and select several values in scanning result, as described anti- Answer the characteristic parameter CS of unit1；
The first liquid is poured out, confining liquid is loaded in the reaction member, by 0-120 hours at 20-30 degrees Celsius, relatively Humidity is to be placed in 70-100 space；
First liquid is placed in the reaction member, with 50mV-10000mV sine voltage by 40Hz-4MHz to anti- Answer unit to do frequency impedance scanning, recording responses electric current and phase difference and select several values in scanning result, as described anti- Answer the characteristic parameter CS of unit2；And
The first liquid is poured out, examines thing to be put into the reaction member target for including bacterial antigens of various concentrations It is interior, inspection is performed, under the conditions of various concentrations, obtains three characteristic parameter CS respectively0、CS1、CS2As described under respective concentration The coating parameter of reaction member.
Optionally, it is described to pour out the first liquid in the method for above-mentioned detection bacterium antigen, the bacterium of various concentrations is resisted Former probe solution is placed in the reaction member, and by 0-120 hours at 20-30 degrees Celsius, relative humidity is to be put in 70-90 space Also include after the step of putting：
10Hz~10MHz excitation letter is applied to the reaction member of the bacterial antigens probe solution containing various concentrations Number.
Optionally, in the method for above-mentioned detection bacterium antigen, the step of the coating parameter for obtaining the reaction member Suddenly also include：
Obtaining characteristic parameter CS0Afterwards, cleaning liquid is placed in the reaction member to be detected, to described to be detected Reaction member is cleaned, and the cleaning liquid includes any of deionized water, ultra-pure water, buffer solution and running water；
Obtaining characteristic parameter CS1Afterwards, cleaning liquid is placed in the reaction member to be detected, to described to be detected Reaction member is cleaned；And
Obtaining characteristic parameter CS2Afterwards, cleaning liquid is placed in the reaction member to be detected, to described to be detected Reaction member is cleaned.
Optionally, in the method for above-mentioned detection bacterium antigen, the method for the detection bacterium antigen also includes：
Examine thing to pre-process the target that can be in the range of concentrations, the pretreatment include filtering out it is described can Target in the range of concentrations examines the particulate matter in thing.
Optionally, in the method for above-mentioned detection bacterium antigen, it is described determine that the target examines thing can concentrations The step of scope, includes：
Choose concentration be ng/mL target examine thing, be diluted to 1ag/mL from ng/mL by exponential, using 10Hz~ 10MHz pumping signal examines the bacterial antigens in thing to carry out full scan to the target, obtains the response of the bacterial antigens Parameter and corresponding target examine the concentration range of thing, wherein, n >=0.5；
Response parameter and corresponding target to the bacterial antigens examine the relation of the concentration range of thing to be fitted, and obtain Take fitting function；
According to the fitting function, obtain the target and examine the concentration range of thing, and/or obtained according to the fitting function Take the response parameter of the bacterial antigens.
Optionally, in the method for above-mentioned detection bacterium antigen, the measuring unit work of the reaction member connection, obtain The target examines the step of data message of the bacterial antigens in thing to include：
Apply 10Hz~10MHz pumping signal to reaction member by measuring unit, meanwhile, detect the resistance of reaction member Anti- value changes, the impedance value of the reaction member is transmitted to a memory cell and stored.
Optionally, in the method for above-mentioned detection bacterium antigen, the data message in the analysis memory cell, obtain The step of taking testing result includes：
Data processing, including denoising and amendment are carried out to the impedance value of the reaction member；
By the impedance value of the reaction member after data processing, testing result is obtained, and the testing result is shown in One display unit.
In summary, the present invention proposes a kind of method of detection bacterium antigen, by being coated with bacterial antigens probe in advance Into a reaction member so that detection time is shorter, and accuracy of detection is higher, and the professional technique of operating personnel is required to reduce, It is simple to operate；Further, target inspection thing is placed in the reaction member for being coated with bacterial antigens probe, passes through measuring unit Examine the bacterial antigens in thing to detect the target in the reaction member, and detection information stored and analyzed, The testing result of bacterial antigens is obtained, testing result is easy to achieve, and is easy to testing result of the those skilled in the art to storage Analyzed, reduce testing cost.
Brief description of the drawings
Fig. 1 is the bacterial antigens detection method flow chart in one embodiment of the present invention；
Fig. 2 is the flow chart for obtaining the method for the coating parameter of the bacterial antigens probe of embodiment in Fig. 1；
Fig. 3 is the measuring unit structural representation in one embodiment of the present invention.
Specifically, 1- measuring units；11- signaling modules；12 detection modules；2- memory cell；3- display units；4- reacts Unit.
Embodiment
The embodiment of the present invention is described in more detail below in conjunction with schematic diagram.According to description below and Claims, advantages and features of the invention will become apparent from.It should be noted that accompanying drawing is using very simplified form and Using non-accurately ratio, only for the purpose of facilitating and clarifying the purpose of the embodiments of the invention.
The present invention proposes a kind of bacterial antigens detection method, for detecting the bacterial antigens in target inspection thing, operation letter Single, detection time is shorter, and accuracy of detection is higher, and the professional technique of operating personnel is required to reduce.Specifically, with reference to figure 1 and Fig. 3, the bacterial antigens detection method in one embodiment of the present invention include：
Step S1：Determine that the target examines thing can concentrations scope.
In a preferred embodiment, choose the target that concentration is 10g/mL and examine thing, it is diluted from 10g/mL To 1ag/mL, full scan is carried out to bacterial antigens using 10Hz~10MHz pumping signal, chooses the response of the bacterial antigens Parameter, it is determined that corresponding target examines the concentration range of thing；
Response parameter and corresponding target to the bacterial antigens examine the relation of the concentration range of thing to be fitted, and obtain Take fitting function；
According to the fitting function, obtain the target and examine the concentration range of thing, and/or obtained according to the fitting function Take the response parameter of the bacterial antigens..
Optionally, dilute the target that the concentration is 10g/mL examine thing can be, but not limited to include by it is exponential by its from 10g/mL is diluted to 1ag/mL, i.e. concentration after dilution next time is 1/10th of the concentration after this dilution.The present invention is to dilute Releasing the target that the concentration is 10g/mL examines method and multiple of thing etc. to be not intended to be limited in any.
Step S2：A reaction member 4 is obtained, obtains a reaction member 4, the reaction member 4 is coated with bacterium and resisted in advance Former probe, for detecting the bacterial antigens in the target inspection thing.
Specifically, the reaction member 4 includes a reaction chamber, the bacterial antigens probe is coated in the bottom of the reaction chamber On one detecting electrode in portion, the detecting electrode is connected with a measuring unit 1.Specifically, the reaction member is coated with bacterium Antigen probe, and the bacterial antigens probe can examine the bacterial antigens in thing to react with the target, the bacterium resists Former probe includes bacterial antigens antibody corresponding with the bacterial antigens, and the present invention does not do any restrictions to this.
Optionally, at least one layer of sealer is also included on the detecting electrode of the reaction member 4.The sealer is typically adopted Prepared with unrelated protein, prevent other materials to be adsorbed onto on detecting electrode and testing result is impacted, one improves the standard of detection True property.
Include specifically, the reaction member 4 is coated with the step of bacterial antigens probe in advance：
The coating parameter of the reaction member 4 is obtained, according to the coating parameter of the reaction member 4, obtains the bacterium Coating time, temperature and the humidity of antigen probe solution；
The bacterial antigens probe solution is placed in the reaction member 4, according to coating time, temperature and the humidity The reaction member 4 is coated with.
Bacterial antigens probe in the present embodiment is coated on the bacterial antigens probe in advance, on the one hand reduces detection Time, on the one hand the reaction member progress bacterial antigens detection after screening coating, improves accuracy of detection and detection accuracy, right The professional technique requirement of detection operating personnel also decreases.
Step S3：Target inspection thing is placed in the reaction member 4 for being coated with bacterial antigens probe, the bacterium Antigen probe examines the bacterial antigens of thing that immune response occurs with the target, forms bacterial antibodies antigenic compound.
Step S4：The measuring unit 1 being connected with the reaction member 4 works, and obtains the bacterium in the target inspection thing The data message of antigen, and transmit to a memory cell 2 and stored.
Specifically, pumping signal is applied to reaction member 4 by the signaling module 11 in measuring unit 1, meanwhile, detect mould The data message of the detection bacterium antigen of block 12, the data information transfer of the bacterial antigens a to memory cell 2 is stored.
Optionally, the data message of the bacterial antigens in the present invention includes but is not limited to the impedance value of the reaction member The impedance value of the reaction member is transmitted to a memory cell 2 and stored by change, detection module 12.Bacterium in step S3 Antigenic compound adds the thickness of the detecting electrode of the reaction member, and therefore, the impedance value of reaction member can change, Preferably, in one embodiment of the invention, detect the change in impedance value of reaction member, by the impedance value of the reaction member transmit to One memory cell is stored.
S5：The data message in the memory cell 2 is analyzed, obtains testing result.
Specifically, those skilled in the art can analyze and obtain according to the change in impedance value of the reaction member 4 in the present embodiment Testing result, bacterial antigens species that the testing result includes but is not limited to examine for the target in thing, quantity and normal Situation etc.；According to the composition of the analysis bacterial antigens of the bacterial antigen complex and species etc., the present invention is not done to this Any restrictions.
Optionally, data processing, including denoising and amendment are carried out to the impedance value of the reaction member 4, passes through number According to the impedance value of the reaction member after processing, testing result is obtained, is shown in a display unit 3.Optionally, the detection is tied Fruit shows that it is PC or smart mobile phone that the display unit 3, which includes but is not limited to, in order to technical staff by a display unit 3 Inquired about and analyzed, the present invention is not intended to be limited in any to this.
Optionally, in addition to step S6：Thing is examined to pre-process the target that can be in the range of concentrations, it is described Pretreatment includes filtering out the target that can be in the range of concentrations and examines particulate matter in thing, to increase detection accuracy.
In order to increase bacterial antigens accuracy of detection, the target screened in further embodiment of this invention suitable for certain concentration is examined The step reference chart 2 of the coating bacterial antigens probe of thing, specifically, the step of obtaining the coating parameter of the reaction member is wrapped Include：
Step Sa：First liquid is placed in reaction member 4, first liquid includes deionized water, ultra-pure water, buffering Liquid, PBS solution, Buffer B and running water or any combination, 40Hz-4MHz is pressed with 50mV-10000mV sine voltage The scanning of frequency electrical conductivity, recording responses electric current and phase difference are done to each reaction member and selects several in scanning result Value, the characteristic parameter CS as the reaction member0；
Step Sb：The first liquid is poured out, the bacterial antigens probe solution of various concentrations is placed in inside reaction member 4, pressed 0-120 hours, relative humidity was to be placed in 70-90 space at 20-30 degrees Celsius.
Optionally, precision is screened to realize, at 0 hour, 20 degrees Celsius, the space that relative humidity is 70, which is placed, was, 20 degrees Celsius, the space that relative humidity is 70 by the bacterial antigens probe solution of the various concentrations as the moment of reaction member 4, The invention is not limited in this regard.
The bacterial antigens probe includes bacterial antigens antibody corresponding with the bacterial antigens.
In one embodiment of the invention, the bacterial antigens are detected by the impedance value of the reaction member；It is optional , those skilled in the art can obtain the structure that the target examines the bacterial antigens in thing by extracting bacterial antigen complex Into and species etc., further studied.
Step Sc：First liquid is placed in each reaction member, and it is molten that first liquid includes deionized water, ultra-pure water, PBS Any of liquid and Buffer B, make frequency to reaction member by 40Hz-4MHz of 50mV-10000mV sine voltage Rate impedance scan, recording responses electric current and phase difference simultaneously select several values of scanning result, the feature as the reaction member Parameter CS1；
Step Sd：The first liquid is poured out, confining liquid is loaded in reaction member, by 0-120 hours at 20-30 degrees Celsius, Relative humidity is to be placed in 70-100 space；
Step Se：First liquid is placed in each reaction member, 40Hz- is pressed with 50mV-10000mV sine voltage 4MHz does frequency impedance scanning, recording responses electric current and phase difference to reaction member and selects several values of scanning result, makees For the characteristic parameter CS of the reaction member2；
Step Sf：The first liquid is poured out, examines thing to be put into anti-the target of the known various concentrations for including bacterial antigens Answer in unit, perform inspection, under the conditions of various concentrations, obtain three coating parameter CS respectively0、CS1、CS2, as corresponding dense The coating parameter of the lower bacterial antigens probe solution of degree.Reaction member 4 includes corresponding bacterial antigens probe, it is determined that reaction The coating parameter of unit 4, the target as selected suitable for certain concentration examine the bacterial antigens probe of thing.
Optionally, the step of coating parameter of the acquisition reaction member also includes：
Obtaining characteristic parameter CS0Afterwards, cleaning liquid is placed in the reaction member to be detected, to described to be detected Reaction member is cleaned, and the cleaning liquid includes any of deionized water, ultra-pure water, buffer solution and running water；
Obtaining characteristic parameter CS1Afterwards, cleaning liquid is placed in the reaction member to be detected, to described to be detected Reaction member is cleaned；And
Obtaining characteristic parameter CS2Afterwards, cleaning liquid is placed in the reaction member to be detected, to described to be detected Reaction member is cleaned.
Cleaning to reaction member can reduce the other impurities in reaction member, and then add the detection of the reaction member Precision.
The target for being applied to certain concentration by filtering out examines the bacterial antigens probe of thing, and the target of certain concentration is examined The bacterial antigens tested in thing are detected, and are on the one hand coated with bacterial antigens probe in advance, are reduced detection time, on the other hand, The reaction member selected by step Sa- steps Sf, can obtain corresponding standing time, temperature and humidity, increase detection Accuracy, meanwhile, testing process is simple, and the professional technique of operating personnel is required to reduce, so as to reduce testing cost, had Feasibility.
Optionally, in addition to step Sg：The reaction member 4 of the bacterial antigens probe solution containing various concentrations is applied Add 10Hz~10MHz pumping signal, be easy to be internally formed partial fluid caused by significant thermograde in reaction member Flowing, coating speed of the increase bacterial antigens probe solution on detecting electrode, the present invention do not do any restrictions to this.
In summary, the present invention proposes a kind of method of detection bacterium antigen, by being coated with bacterial antigens probe in advance Into a reaction member so that detection time is shorter, and accuracy of detection is higher, and the professional technique of operating personnel is required to reduce, It is simple to operate；Further, target inspection thing is placed in the reaction member for being coated with bacterial antigens probe, passes through measuring unit Examine the bacterial antigens in thing to detect the target in the reaction member, and detection information stored and analyzed, The testing result of bacterial antigens is obtained, testing result is easy to achieve, and is easy to testing result of the those skilled in the art to storage Analyzed, reduce testing cost.
The preferred embodiments of the present invention are above are only, any restrictions effect is not played to the present invention.Belonging to any Those skilled in the art, in the range of technical scheme is not departed from, to the invention discloses technical scheme and Technology contents make the variation such as any type of equivalent substitution or modification, belong to the content without departing from technical scheme, still Belong within protection scope of the present invention.

Claims (10)

  1. A kind of 1. method of detection bacterium antigen, for detecting the bacterial antigens in target inspection thing, it is characterised in that including：
    Determine that the target examines thing can concentrations scope；
    A reaction member is obtained, the reaction member is coated with bacterial antigens probe in advance；
    Target inspection thing is placed in the reaction member, the bacterial antigens probe examines the bacterium in thing with the target Antigen reacts；
    The target is obtained by measuring unit and examines the data message of the bacterial antigens in thing, and transmits to a memory cell Row storage；And
    The data message in the memory cell is analyzed, obtains testing result.
  2. 2. the method for detection bacterium antigen as claimed in claim 1, it is characterised in that the reaction member includes a reaction Chamber, the bacterial antigens probe are coated on a detecting electrode of the bottom of the reaction chamber, the detecting electrode and the survey Measure unit connection.
  3. 3. the method for detection bacterium antigen as claimed in claim 2, it is characterised in that the reaction member is coated with bacterium in advance The step of antigen probe, includes：
    Obtain bacterial antigens probe solution corresponding with target inspection thing concentration；
    The coating parameter of the reaction member is obtained, according to the coating parameter of the reaction member, the bacterial antigens is obtained and visits Coating time, temperature and the humidity of pin solution；And
    The bacterial antigens probe solution is placed in the reaction member, according to coating time, temperature and the humidity to institute Reaction member is stated to be coated with；Also include at least one layer of closure on the detecting electrode of the reaction member.
  4. 4. the method for detection bacterium antigen as claimed in claim 3, it is characterised in that obtain the coating ginseng of the reaction member Several steps include：
    First liquid is placed in the reaction member, it is molten that first liquid includes deionized water, ultra-pure water, buffer solution, PBS Any of liquid, Buffer B and running water, the salt content of the ultra-pure water are less than 0.3mg/L, and electrical conductivity is less than 0.2 μ s/cm；
    Make the scanning of frequency electrical conductivity, record to the reaction member by 40Hz-4MHz of 50mV-10000mV sine voltage Response current and phase difference simultaneously select several values in scanning result, the characteristic parameter CS as the reaction member0；
    The first liquid is poured out, the bacterial antigens probe solution of various concentrations is placed in the reaction member, existed by 0-120 hours 20-30 degrees Celsius, relative humidity is to be placed in 70-90 space, and the bacterial antigens probe solution includes resisting with the bacterium Bacterial antigens antibody-solutions corresponding to original；
    First liquid is placed in the reaction member, it is single to reaction by 40Hz-4MHz with 50mV-10000mV sine voltage Member does frequency impedance scanning, recording responses electric current and phase difference and selects several values in scanning result, single as the reaction The characteristic parameter CS of member1；
    The first liquid is poured out, confining liquid is loaded in the reaction member, by 0-120 hours at 20-30 degrees Celsius, relative humidity To be placed in 70-100 space；
    First liquid is placed in the reaction member, it is single to reaction by 40Hz-4MHz with 50mV-10000mV sine voltage Member does frequency impedance scanning, recording responses electric current and phase difference and selects several values in scanning result, single as the reaction The characteristic parameter CS of member2；And
    The first liquid is poured out, examines thing to be put into the reaction member target for including bacterial antigens of various concentrations, holds Performing check, under the conditions of various concentrations, three characteristic parameter CS are obtained respectively0、CS1、CS2As the reaction under respective concentration The coating parameter of unit.
  5. 5. the method for detection bacterium antigen as claimed in claim 4, it is characterised in that it is described to pour out the first liquid, by difference The bacterial antigens probe solution of concentration is placed in the reaction member, by 0-120 hours at 20-30 degrees Celsius, relative humidity 70- Also include after the step of being placed in 90 space：
    10Hz~10MHz pumping signal is applied to the reaction member of the bacterial antigens probe solution containing various concentrations.
  6. 6. the method for detection bacterium antigen as claimed in claim 4, it is characterised in that the bag for obtaining the reaction member Also included the step of parameter：
    Obtaining characteristic parameter CS0Afterwards, cleaning liquid is placed in the reaction member to be detected, to the reaction to be detected Unit is cleaned, and the cleaning liquid includes any of deionized water, ultra-pure water, buffer solution and running water；
    Obtaining characteristic parameter CS1Afterwards, cleaning liquid is placed in the reaction member to be detected, to the reaction to be detected Unit is cleaned；And
    Obtaining characteristic parameter CS2Afterwards, cleaning liquid is placed in the reaction member to be detected, to the reaction to be detected Unit is cleaned.
  7. 7. the method for detection bacterium antigen as claimed in claim 1, it is characterised in that the method for the detection bacterium antigen is also Including：
    Thing is examined to pre-process the target that can be in the range of concentrations, the pretreatment, which includes filtering out, described can detect Target in concentration range examines the particulate matter in thing.
  8. 8. the method for detection bacterium antigen as claimed in claim 1, it is characterised in that described to determine that the target examines thing It can include the step of concentrations scope：
    Choose the target that concentration is ng/mL and examine thing, 1ag/mL is diluted to from ng/mL by exponential, using 10Hz~10MHz's Pumping signal examines the bacterial antigens in thing to carry out full scan to the target, obtains the response parameter and phase of the bacterial antigens The target answered examines the concentration range of thing, wherein, n >=0.5；
    Response parameter and corresponding target to the bacterial antigens examine the relation of the concentration range of thing to be fitted, and obtain and intend Close function；
    According to the fitting function, the concentration range that the target examines thing is obtained, and/or institute is obtained according to the fitting function State the response parameter of bacterial antigens.
  9. 9. the method for detection bacterium antigen as claimed in claim 1, it is characterised in that the measurement list of the reaction member connection Member work, obtaining the step of target examines the data message of bacterial antigens in thing includes：
    Apply 10Hz~10MHz pumping signal to reaction member by measuring unit, meanwhile, detect the impedance value of reaction member Change, the impedance value of the reaction member is transmitted to a memory cell and stored.
  10. 10. the method for detection bacterium antigen as claimed in claim 9, it is characterised in that in the analysis memory cell Data message, obtain testing result the step of include：
    Data processing, including denoising and amendment are carried out to the impedance value of the reaction member；
    By the impedance value of the reaction member after data processing, testing result is obtained, and the testing result is shown in one and shown Show unit.
CN201710642999.3A 2017-07-31 2017-07-31 The detection method and bacterial antigens probe method for coating of a kind of bacterial antigens Pending CN107367613A (en)

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