CN104634968A - Biosensor system for pathogenic bacterium detection - Google Patents
Biosensor system for pathogenic bacterium detection Download PDFInfo
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- CN104634968A CN104634968A CN201510041227.5A CN201510041227A CN104634968A CN 104634968 A CN104634968 A CN 104634968A CN 201510041227 A CN201510041227 A CN 201510041227A CN 104634968 A CN104634968 A CN 104634968A
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- detection
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56911—Bacteria
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
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- Immunology (AREA)
- Engineering & Computer Science (AREA)
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- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
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- General Health & Medical Sciences (AREA)
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- Pathology (AREA)
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- Biochemistry (AREA)
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- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Inorganic Chemistry (AREA)
- Investigating Or Analyzing Materials By The Use Of Magnetic Means (AREA)
Abstract
The invention relates to a biosensor system for detecting pathogenic bacteria. The biosensor system comprises two parts, wherein the first part is a biosensor platform which is composed of a magnetostriction material that is bound with an antibody on the surface, a Helmholtz coil and a closed microphone, and the second part is an information analysis system composed of a direct-current power supply, an electric wire, a coil and a lock-in amplifier or a network analyzer (AV3620) or an impedance analyzer (Agilent 4294A). The detection limit of a biosensor disclosed by the invention is 100cfu/mL, and the detection time can vary with the change in the viscosity of a detected solution and is generally 20-60min. Therefore, the biosensor disclosed by the invention has the advantages of short detection time, low detection limit and the like, and is capable of conveniently, rapidly and accurately detecting the pathogenic bacteria in a liquid or solid sample; and the biosensor system is also suitable for portable application.
Description
Technical field
The present invention relates to detection technique field, be specifically related to the bio-sensor system for pathogenic microbes detect.
Background technology
The routine analyzer of the object had in multiple detection test sample book known in the state of the art.Such as, utilize immune specific reaction mechanism, determined existence or the quantity of antigen by the painted of antagonist or molecular labeling.The present invention utilizes immune this specific reaction mechanism, antibody is bundled in the surface of magnetostriction materials by molecular modification, after antigen is combined with antibody, the frequency of its material changes, by existence or the quantity of information system analysis determination antigen.
Recently there will be a known magnetostriction materials bio-sensing, and its functional material that magnetostriction materials are changed as energy and information, namely it can be used as the sensitive element that sensor information is changed.And biology sensor of the present invention is using the platform of magnetostriction materials as biology sensor.
The magnetostriction materials of magnetostrictive material biosensor known are recently by molecular modification, and its reactive group is sulfydryl, and it is generally all used on the electrode of electrochemical reaction.
In microfluidic assay, used magnetic mark to come existence or the quantity of detect analytes recently.Magnetic mark is used as such as magnetic-particle, also claims magnetic bead, actuating magnetic particles can be carried out by applying magnetic field, thus can analysis process be accelerated.The identification of magnetic bead adopts Systems for optical inspection.
Recently known being joined by bacterium liquid to be measured in the colloid gold particle solution of aptamer containing objective microbe and aptamer complementary strand is hatched, described aptamer complementary strand is connected to colloid gold particle I surface, and being combined with the identification of aptamer by objective microbe is that the state of faling apart of gathering of colloid gold particle changes thus the change producing solution colour judges testing result.
Known biosensors catches sample detection liquid recently, arranges blank and the positive concentrations control of standard items simultaneously, starts adenosine triphosphate synthetic reaction, then detect with Fluorescence Scanner.
Summary of the invention
The object of this invention is to provide a kind of can the bio-sensor system of pathogenic bacteria in tracer liquid or solid sample.In order to realize object of the present invention, intend adopting following technical scheme:
One aspect of the present invention relates to a kind of magnetostrictive material biosensor system for bacterial detection, comprising:
The antibody of magnetostriction materials and surface binding;
Working coil, Helmholtz coils, electric power connection line, and direct supply is provided;
Information analysis system, described information analysis system comprises network analysis system and impedance analysis system.The existing 3 kinds of methods of information analysis: lock-in amplifier method, network analyzer (AV3620) method and electric impedance analyzer (Agilent 4294A) method.Preferred embodiment is network analyzer method.
In a preferred embodiment of the present invention, it is characterized in that described working coil material used can cause the alloy material of resonance with magnetostriction materials.Magnetostriction materials of the present invention are under magnetic fields, the class material that its length, width and height all can change, the magnetostriction materials used in the implementation case are often magnetostriction particulate (MSP) or are magnetostriction micro-cantilever (MSMC), and generally its optimum length is the diameter of winding wire.
In a preferred embodiment of the present invention, it is characterized in that described magnetostriction materials Surface coating SiO
2shell, then derivative amino at its molecular surface, then by hydroxy amino, bind antibody by hydroxyl.
The present invention also relates to the method for pathogenic bacteria in the magnetostrictive material biosensor systems axiol-ogy sample adopting above-mentioned bacterial detection on the other hand, it is characterized in that comprising the steps:
There are the magnetostriction materials of antibody to be positioned in working coil binding, allow sample slowly flow across magnetic stripe by pipeline, the germ in sample is combined with its antibody;
The change of the resonant frequency that detection magnetic stripe and coil produce, after infosystem process, can determine the quantity of germ.
In a preferred embodiment of the present invention, it is characterized in that described method is for following detection:
-for the detection of pathogenic bacteria in the food liquids such as beverage, milk, grease,
-for eating the pathogenic microbes detect of fruit and vegetable surfaces raw.
-for the detection of bacterium in potable water.
-for the detection of pathogenic bacteria in human body urine sample.
-for the pathogenic bacteria diagnostic tool of food and environment diagnosis.
The information analysis system of biology sensor of the present invention comprises network analysis system and impedance analysis system etc., usual network analysis system adopts vector network analyzer to carry out frequency sweep detection, also can build simple circuit, use the chip such as amplitude and phase measurement to detect the amplitude variations of magnetostriction materials.Because network analysis system volume ratio is comparatively large, expensive, so need to simplify procedures in portable use.
The detection of biology sensor of the present invention is limited to 100cfu/mL, and detection time is different because of the difference of detection solution viscosity, generally at 20-60min.Bio-sensor system disclosed in this invention can the bacterium of tracer liquid or solid surface, and method is easy, quick, accurate, is also applicable to portable use.
Accompanying drawing explanation
Fig. 1 is magnetostrictive material biosensor tracer liquid sample of the present invention diagram;
Fig. 2 is coil and magnetic stripe annexation schematic diagram, and the specification of magnetic stripe is variant because of the difference of testing goal thing.
Embodiment
If not specified, the conventional means that technological means used in embodiment is well known to those skilled in the art.
Adopt bio-sensor system of the present invention to the embodiment comprising listerial aqueous solution and detect:
Step one, magnetostriction materials binding antibody
Magnetostriction materials are wrapped SiO2 shell, then derivative amino at its molecular surface, then by hydroxy amino, after adding catalyzer, bind antibody.
The combination of step 2, antibody and antigen
There is the magnetic stripe of antibody to be positioned in coil binding, allow aqueous solution slowly flow across magnetic stripe by pipeline (being made up of leather hose and glass tube), the Listeria in aqueous solution is combined with its antibody.
Step 3, detection
The change of the resonant frequency produced by magnetic stripe and coil, after infosystem process, can determine listerial quantity.
Step 4, testing result are as follows:
Biology sensor detects listerial dynamic frequency
Be not difficult to find out from the above results, along with the increase of bacterial concentration, frequency diminishes the testing result of this sample, frequency kept stable when reaching higher level to concentration.
Utilize bio-sensor system of the present invention to the embodiment of apple surface pathogenic microbes detect:
The immunologic opsonin reaction of step one, apple surface
First apple is positioned in inspection instrument, then 55 pieces of bindings is had the magnetic stripe of antibody (wherein 50 pieces is detect use, and 5 pieces is contrast) to be sprinkling upon the surface of apple, wait for 1min, allow the antibody that magnetic stripe is bound be combined with salmonella.
Step 2, testing result
Wireless detector detects 55 each 1min of magnetic stripe.
Step 3, data processing and result judge
If when detecting sensor is identical with the resonant frequency of contrast, then think that apple does not have salmonella, if detection peak is lower than contrast, then defining apple has salmonella infection.
Step 4, reclaim the magnet of apple surface with electromagnet.
Testing result is as follows:
Detecting sensor has identical resonant frequency with contrast, and result determination apple does not have salmonella infection.
The above is the preferred embodiments of the present invention; it should be pointed out that for those skilled in the art, under the prerequisite not departing from principle of the present invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (5)
1., for a magnetostrictive material biosensor system for bacterial detection, comprising:
The antibody of magnetostriction particulate and surface binding;
Working coil, Helmholtz coils, electric power connection line, and direct supply is provided;
Information analysis system, described information analysis system comprises lock-in amplifier, network analyzer (AV3620) or electric impedance analyzer (Agilent 4294A); Preferably, described information analysis system is network analysis system.
2. magnetostrictive material biosensor system according to claim 1, is characterized in that described working coil material used can cause the alloy material of resonance with magnetostriction materials.
3. magnetostrictive material biosensor system according to claim 1, is characterized in that described magnetostriction materials Surface coating SiO
2shell, then derivative amino at its molecular surface, then by hydroxy amino, bind antibody by hydroxyl.
4. adopt the method for pathogenic bacteria in the magnetostrictive material biosensor systems axiol-ogy sample of above-mentioned bacterial detection, it is characterized in that comprising the steps:
There are the magnetostriction materials of antibody to be positioned in working coil binding, allow sample slowly flow across magnetic stripe by pipeline, the germ in sample is combined with its antibody;
The change of the resonant frequency that detection magnetic stripe and coil produce, after infosystem process, can determine the quantity of germ.
5. mode according to claim 4, is characterized in that described method is for following detection:
-for the detection of pathogenic bacteria in beverage, milk, grease food liquid;
-for eating the pathogenic microbes detect of fruit and vegetable surfaces raw;
-for the detection of bacterium in potable water;
-for the detection of pathogenic bacteria in human body urine sample;
-for the pathogenic bacteria diagnostic tool of food and environment diagnosis.
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| CN201510041227.5A CN104634968A (en) | 2015-01-27 | 2015-01-27 | Biosensor system for pathogenic bacterium detection |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105974112A (en) * | 2016-04-28 | 2016-09-28 | 太原科技大学 | Mobile controllable wireless passive magnetostrictive micro detector and detection method thereof |
| CN107064287A (en) * | 2017-05-24 | 2017-08-18 | 吴佩萱 | Germ content device for fast detecting and detection method in liquid |
| CN116448867A (en) * | 2023-06-16 | 2023-07-18 | 北京市农林科学院智能装备技术研究中心 | Rumen liquid pH value wireless passive sensor and monitoring system |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105974112A (en) * | 2016-04-28 | 2016-09-28 | 太原科技大学 | Mobile controllable wireless passive magnetostrictive micro detector and detection method thereof |
| CN107064287A (en) * | 2017-05-24 | 2017-08-18 | 吴佩萱 | Germ content device for fast detecting and detection method in liquid |
| CN107064287B (en) * | 2017-05-24 | 2017-12-29 | 吴佩萱 | Germ content device for fast detecting and detection method in liquid |
| CN116448867A (en) * | 2023-06-16 | 2023-07-18 | 北京市农林科学院智能装备技术研究中心 | Rumen liquid pH value wireless passive sensor and monitoring system |
| CN116448867B (en) * | 2023-06-16 | 2023-10-20 | 北京市农林科学院智能装备技术研究中心 | Rumen liquid pH value wireless passive sensor and monitoring system |
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