CN107375377A - A kind of livestock and poultry echinacea root Chinese medical extract and preparation method and application - Google Patents
A kind of livestock and poultry echinacea root Chinese medical extract and preparation method and application Download PDFInfo
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- CN107375377A CN107375377A CN201710574894.9A CN201710574894A CN107375377A CN 107375377 A CN107375377 A CN 107375377A CN 201710574894 A CN201710574894 A CN 201710574894A CN 107375377 A CN107375377 A CN 107375377A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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Abstract
The present invention provides a kind of livestock and poultry echinacea root Chinese medical extract and preparation method and application.By weight percentage, including：Biology enzyme 5% 15%, oligosaccharide 7% 21%, xylitol 3 7%, remaining is echinacea root.The livestock and poultry of the present invention have immunostimulating effect with echinacea root Chinese medical extract to the livestock and poultry for immunosupress phenomenon occur.
Description
Technical field
It is especially a kind of to be used for what fowl poultry immune was adjusted the present invention relates to a kind of echinacea root Chinese medical extract and its application
Traditional Chinese veterinary medicine extract and application.
Background technology
China has abundant herbal medicine resources advantage and sturdy Research foundation, has including such as Radix Astragali, ginseng etc.
It is improved the research of the herbal medicine of immunologic function.In actual breeding process, the use of herbal medicine, to improve the immune work(of livestock and poultry
For the purpose of energy, the puzzlement of inhibitive ability of immunity disease can be prevented and treated, improve immune effect of vaccine, be ensure aquaculture industry of China health,
Quickly, the primary selection of stable development.
Echinacea (Echinacea pupurea (L.) Moench.) is under the jurisdiction of composite family (Compositae) genus echinacea
(Echinacea), herbaceos perennial, the U.S. central and east and southern Canada area are originated in.Because it has stronger exempt from
Epidemic disease strengthen and antiinflammatory action and paid attention to extensively.European many national and Oceanian New Zealand and Australia all introduce a fine variety cultivation
Training.One of maximum vegetable drug of international market demand amount is turned at present.According to U.S.'s Whole Food magazine rans, 1995~
The plant in 1997 occupies first in continuous 3 years on U.S.'s herbal medicine market sales revenue arrangement list.The category shares 8 kinds and several mutation,
Having developed now medicinal has Echinacea, narrow leaf Echinacea (Echinacea angustifolia DC.) and white Echinacea
Three kinds of (Echinacea pallida Nutt.).In recent years report is also introduced a fine variety in China.The Research Literature of Echinacea is more, mainly
Concentrate on pharmacology, Phytochemistry, cultivation etc..Echinacea is widely used in west, is world-famous " immune " grass
Medicine, there is prominent anti-infective and immunologic enhancement, can by stimulate the immune system of body strengthen body to bacterium and
The resistance of virus infection.
The main medicinal part of Echinacea is under ground portion (root, rhizome), stem or aerial part, mainly contains polysaccharide, coffee
More constituents such as acid derivative, volatile oil, more carbene, alkylamide and flavonoids, it is good immunomodulator and stimulant,
Cold, inflammation, rheumatism and abscess of throat can be treated, is that the only a few found in the world so far has immunity increasing
One of strong and anti-inflammatory double action autonomic drug, therapeutic domain is wider, the effect of having extensive therapeutic domain and stablize.Purple cone
Chrysanthemum extract (Echinacea aerial part, echinacea root, narrow leaf echinacea root etc.), formulations sold volume are ranked first for many years,
America and Europe is greatly paid close attention to, and current foreign countries there are about 300 kinds of Echinaceas or its compound preparation is used for clinic, and Echinacea preparation is complete
There is the trend become increasingly popular in the range of ball.Since Echinacea introduces China, the plant is progressively considered as and traditional antiviral immunity
Enhancing Chinese herbal medicine astragalus has the function that similar, the extensive use in antiviral Chinese herbal medicine.
The content of the invention
The technical problems to be solved by the invention are a kind of for the echinacea root Chinese medical extract of livestock and poultry and application.
The technology used in the present invention is：A kind of livestock and poultry echinacea root Chinese medical extract, the raw material mainly used is 1 year
Echinacea root is dried more than raw.
A kind of livestock and poultry echinacea root Chinese medical extract, by weight percentage, including：Biology enzyme 5%-15%, oligosaccharide
7%-21%, xylitol 3-7%, remaining is echinacea root.
The present invention also provides a kind of preparation method of livestock and poultry with echinacea root Chinese medical extract, including：
Step A：Echinacea root crushes, and crosses mesh sieve, adds biology enzyme, oligosaccharide, xylitol, is fermented in fermentation tank；
Step B：Add alcohol reflux to extract in resulting fermentate, filter, then filtrate is adjusted into PH values, stand, mistake
Filter, obtained precipitation are dried under reduced pressure, and are crushed, and are sieved, and are mixed, that is, are obtained livestock and poultry echinacea root Chinese medical extract.
Preferably, in the step A, mesh sieve uses 10-20 mesh.
Preferably, in the step B, the mode of anaerobic fermentation is taken in fermentation, and the time of continuously fermenting is 12-36 hours, PH
For 2-4.
Preferably, in the step B, the volumetric concentration of the ethanol is 20%-90%；The addition volume of ethanol is described
10-20 times of fermentate；
Preferably, in the step B, heating and refluxing extraction temperature is 40 DEG C -95 DEG C, and extraction time is 1-2 hours, extraction
Number is 2-4 times.
The present invention has the advantages that：
1st, extracting method of the invention first carries out solid fermentation, and compared with conventional method, extraction efficiency greatly increases, and has
Imitate active component witloof acid content and improve about 30%.
2nd, livestock and poultry of the invention have immune thorn with echinacea root Chinese medical extract to there are the livestock and poultry of immunosupress phenomenon
Swash effect.
3rd, echinacea root Chinese medical extract of the invention, it is easy to use available for drinking water administration；
4th, the medicinal material used in the present invention is Echinacea rhizome, belongs to the utilization of resources, and use cost is low, and preparation method is simple,
Stability is good, is easy to transport and preserves, and has preferable application prospect.
5th, this product with other antibiotics compatible uses, can reduce the use of antibiotics dosage, reduce
Medicament residue.
Brief description of the drawings
Fig. 1,2 Echinacea Purpurea Herb P.E indentification by TLC results.Wherein, 1. reference substance one, 3. reference substances two, 2,4,5.
Embodiment sample 1-3.
Fig. 3 Cichoric acid reference substance high-efficient liquid phase chromatograms.
The sample Cichoric acid high-efficient liquid phase chromatogram of Fig. 4 embodiments 1.
The sample Cichoric acid high-efficient liquid phase chromatogram of Fig. 5 embodiments 2.
The sample Cichoric acid high-efficient liquid phase chromatogram of Fig. 6 embodiments 3.
The sample Cichoric acid high-efficient liquid phase chromatogram of Fig. 7 embodiments 4.
The sample Cichoric acid high-efficient liquid phase chromatogram of Fig. 8 embodiments 5.
Fig. 9 traditional extraction process extracts sample Cichoric acid high-efficient liquid phase chromatogram.
Embodiment
The preferably embodiment of the present invention is described in further detail below：
Embodiment 1
Step A：Echinacea root 200g is dried, is crushed, 10 mesh sieves is crossed, is put into solid-state fermenter, adds percentage by weight
Than 6% amylase, 7% stachyose and 3% xylitol, it is sprinkling upon above after well mixed, closed, the time of continuously fermenting is small for 24
When, PH 3.8.
Step B：Fermentate adds volumetric concentration to be 30% ethanol, 80 DEG C of refluxing extractions, and extraction 1 hour, continuously extracts 3 every time
Secondary, filtering, filtrate adjusts pH value to 2.0 with sulfuric acid, stands, filtering, precipitation is dried under reduced pressure, and is crushed, and is sieved, and is mixed, is produced.
Embodiment 2
Step A：Echinacea root 200g is dried, is crushed, 10 mesh sieves is crossed, is put into solid-state fermenter, adds percentage by weight
Than 8% catalase, 9% stachyose and 5% xylitol, it is sprinkling upon above after well mixed, closed, the time of continuously fermenting is 24
Hour, PH 3.8.
Step B：Fermentate adds volumetric concentration 40% ethanol, 80 DEG C of refluxing extractions, every time extraction 2 hours, continuous to extract 2 times,
Filtering, filtrate adjust pH value to 3.0 with sulfuric acid, stood, filtering, precipitation is dried under reduced pressure, and is crushed, and is sieved, and is mixed, is produced.
Embodiment 3
Step A：Echinacea root 200g is dried, is crushed, 10 mesh sieves is crossed, is put into solid-state fermenter, adds percentage by weight
Than 10% lipase, 9% stachyose and 4% xylitol, it is sprinkling upon above after well mixed, closed, the time of continuously fermenting is small for 24
When, PH 3.8.
Step B：Fermentate adds volumetric concentration 60% ethanol, 90 DEG C of refluxing extractions, every time extraction 2 hours, continuous to extract 2 times,
Filtering, filtrate adjust pH value to 3.0 with sulfuric acid, stood, filtering, precipitation is dried under reduced pressure, and is crushed, and is sieved, and is mixed, is produced.
Embodiment 4
Step A：Echinacea root 200g is dried, is crushed, 20 mesh sieves is crossed, is put into solid-state fermenter, adds percentage by weight
Than 10% zytase, 9% FOS and 4% xylitol, it is sprinkling upon above after well mixed, closed, the time of continuously fermenting is
24 hours, PH 3.8.
Step B：Fermentate adds volumetric concentration 40% ethanol, 90 DEG C of refluxing extractions, every time extraction 1 hour, continuous to extract 3 times,
Filtering, filtrate adjust pH value to 3.0 with sulfuric acid, stood, filtering, precipitation is dried under reduced pressure, and is crushed, and is sieved, and is mixed, is produced.
Embodiment 5
Step A：Echinacea root 200g is dried, is crushed, 10 mesh sieves is crossed, is put into solid-state fermenter, adds percentage by weight
Than 10% zytase, 9% galactooligosaccharide and 4% xylitol, it is sprinkling upon above after well mixed, it is closed, continuously ferment the time
For 24 hours, PH 3.8.
Step B：Fermentate adds volumetric concentration 40% ethanol, 92 DEG C of refluxing extractions, every time extraction 1 hour, continuous to extract 3 times,
Filtering, filtrate adjust pH value to 3.0 with sulfuric acid, stood, filtering, precipitation is dried under reduced pressure, and is crushed, and is sieved, and is mixed, is produced.
The Piglet s colibacillosis of embodiment 6
By echinacea root Chinese medical extract made from embodiment 1, embodiment 2, embodiment 5 (individually below correspondingly referred to as
Test group 1,2,3) and purchase echinacea root powder (hereinafter referred to as control group) be applied to it is immune after chicken group, compare it to poultry
Fowl is immunized whether front and rear antibody level has facilitation effect.
Echinacea root powder：Belong to Echinacea rhizome and directly crush the powder formed.
For trying chicken：Fast rheum officinale plumage baby chick 100, is fed with material and is free of any medicine for autogamy, drink clean tap water,
It is standby when supporting to 10 age in days.
For reagent thing：Echinacea root Chinese medical extract, echinacea root powder.
Operating method：Newcastle disease inactivated vaccine is inoculated with 3 ages in days, is used by operation instructions.
Experiment packet and processing：By above-mentioned 10 age in days for examination chicken, be randomly divided into 5 groups, every group 20, it is specific as follows shown in.
1st group：For negative control group, immunity inoculation newcastle disease inactivated vaccine, any medicine is not added.
2nd group：The sample of embodiment 1, drink is mixed, every liter of water adds 0.5g, and experiment begins to use, is used in conjunction 21 days for first day.
3rd group：The sample of embodiment 2, drink is mixed, every liter of water adds 0.5g, and experiment begins to use, is used in conjunction 21 days for first day.
4th group：The sample of embodiment 5, drink is mixed, every liter of water adds 0.5g, and experiment begins to use, is used in conjunction 21 days for first day.
5th group：Echinacea root powder drug control group, mixed feeding, per kilogram feed addition 5g, experiment begin to use for first day,
It is used in conjunction 21 days.
Evaluation index：
(1) death condition
(2) Gain weight
(3) price of deed
(4) antibody level
Result of the test is as shown in table 1：
Table 1
As can be seen from Table 1：
1st, test group chicken growth performance is preferable, has obvious difference compared with negative control group and drug control group.
2nd, antibody IgY against chicken Newcastle Disease level is substantially better than negative control group and drug control group.
3rd, the test group price of deed is high compared with negative control group and drug control group, but difference is not notable.
As shown in Fig. 3-Fig. 7, extracting method extraction sample of the present invention and traditional extraction process sample principle active component chrysanthemum
The assay data of lettuce acid are as shown in table 2：
Table 2
It can be seen from table 2 and Fig. 1-9 compared with traditional extraction process, the extracting method extraction of the invention used
Sample principle active component witloof acid content improves about 30%, and this explanation, this extracting method can greatly improve main in echinacea root
Want the recovery rate of active ingredient.Indentification by TLC and assay are carried out according to the quality standard of formulation, meets regulation.
As a result show, compared with echinacea root powder, described echinacea root Chinese medical extract is to being immunized chicken antibody water
It is flat that there is preferable enhancing effect, there is wide application prospect.
Above content is to combine specific preferred embodiment further description made for the present invention, it is impossible to is assert
The specific implementation of the present invention is confined to these explanations.For general technical staff of the technical field of the invention,
On the premise of not departing from present inventive concept, some simple deduction or replace can also be made, should all be considered as belonging to the present invention's
Protection domain.
Claims (8)
- A kind of 1. livestock and poultry echinacea root Chinese medical extract, it is characterised in that by weight percentage, including：Biology enzyme 5%- 15%th, oligosaccharide 7%-21%, xylitol 3-7%, remaining is echinacea root.
- A kind of 2. preparation method of livestock and poultry echinacea root Chinese medical extract as claimed in claim 1, it is characterised in that bag Include：Step A：Echinacea root crushes, and crosses mesh sieve, adds biology enzyme, oligosaccharide, xylitol, is fermented in fermentation tank；Step B：Add alcohol reflux to extract in resulting fermentate, filter, then filtrate is adjusted into pH value, stand, filtering, obtain Precipitation be dried under reduced pressure, crush, sieve, mix, that is, obtain livestock and poultry echinacea root Chinese medical extract.Step C：According to the quality standard of foundation, active ingredient quality inspection is carried out, packaging, is produced.
- 3. method as claimed in claim 2, it is characterised in that in the step A, mesh sieve uses 10-20 mesh.
- 4. method as claimed in claim 2, it is characterised in that in the step B, the mode of anaerobic fermentation is taken in fermentation, even Continuous fermentation time is 12-36 hours, PH 3.8.
- 5. method as claimed in claim 2, it is characterised in that in the step B, the volumetric concentration of the ethanol is 20%- 90%；The addition volume of ethanol is 10-20 times of the fermentate.
- 6. method as claimed in claim 2, it is characterised in that in the step B, heating and refluxing extraction temperature is 40 DEG C -95 DEG C, extraction time is 1-2 hours, and extraction time is 2-4 times.
- 7. method as claimed in claim 2, it is characterised in that in the step C, active ingredient is mainly Cichoric acid, quality The method of inspection is high performance liquid chromatography.
- 8. application of the livestock and poultry as claimed in claim 1 with echinacea root Chinese medical extract in livestock and poultry medication.
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| CN201710574894.9A CN107375377A (en) | 2017-07-14 | 2017-07-14 | A kind of livestock and poultry echinacea root Chinese medical extract and preparation method and application |
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| CN201710574894.9A CN107375377A (en) | 2017-07-14 | 2017-07-14 | A kind of livestock and poultry echinacea root Chinese medical extract and preparation method and application |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115702912A (en) * | 2021-08-13 | 2023-02-17 | 华南农业大学 | Method for extracting polyphenol from echinacea purpurea stems by using response surface method and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102100718A (en) * | 2009-12-16 | 2011-06-22 | 青岛康地恩药业有限公司 | Process for preparing echinacea purpurea extracts |
| CN102716164A (en) * | 2012-06-28 | 2012-10-10 | 齐鲁动物保健品有限公司 | Echinacea extract and preparation method of echinacea extract |
-
2017
- 2017-07-14 CN CN201710574894.9A patent/CN107375377A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102100718A (en) * | 2009-12-16 | 2011-06-22 | 青岛康地恩药业有限公司 | Process for preparing echinacea purpurea extracts |
| CN102716164A (en) * | 2012-06-28 | 2012-10-10 | 齐鲁动物保健品有限公司 | Echinacea extract and preparation method of echinacea extract |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115702912A (en) * | 2021-08-13 | 2023-02-17 | 华南农业大学 | Method for extracting polyphenol from echinacea purpurea stems by using response surface method and application thereof |
| CN115702912B (en) * | 2021-08-13 | 2024-03-12 | 华南农业大学 | Method for extracting polyphenol in Echinacea purpurea stems by using response surface method and application thereof |
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Application publication date: 20171124 |