CN102100718A - Process for preparing echinacea purpurea extracts - Google Patents
Process for preparing echinacea purpurea extracts Download PDFInfo
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- CN102100718A CN102100718A CN 200910255686 CN200910255686A CN102100718A CN 102100718 A CN102100718 A CN 102100718A CN 200910255686 CN200910255686 CN 200910255686 CN 200910255686 A CN200910255686 A CN 200910255686A CN 102100718 A CN102100718 A CN 102100718A
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- echinacea
- filtrate
- extract
- medicinal residues
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- 235000014134 echinacea Nutrition 0.000 title claims abstract description 37
- 239000000284 extract Substances 0.000 title claims abstract description 15
- 240000004530 Echinacea purpurea Species 0.000 title claims abstract description 11
- 238000004519 manufacturing process Methods 0.000 title description 3
- 239000000706 filtrate Substances 0.000 claims abstract description 27
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 25
- 238000001291 vacuum drying Methods 0.000 claims abstract description 10
- 238000000034 method Methods 0.000 claims abstract description 9
- 244000133098 Echinacea angustifolia Species 0.000 claims description 32
- 235000020694 echinacea extract Nutrition 0.000 claims description 23
- 238000010992 reflux Methods 0.000 claims description 12
- 238000010438 heat treatment Methods 0.000 claims description 9
- 239000003814 drug Substances 0.000 claims description 4
- 239000012567 medical material Substances 0.000 claims description 4
- 239000012141 concentrate Substances 0.000 claims description 2
- 229940079593 drug Drugs 0.000 claims description 2
- 239000002893 slag Substances 0.000 claims description 2
- 238000002481 ethanol extraction Methods 0.000 claims 1
- 238000002360 preparation method Methods 0.000 abstract description 9
- 238000001914 filtration Methods 0.000 abstract description 7
- YDDGKXBLOXEEMN-UHFFFAOYSA-N Di-E-caffeoyl-meso-tartaric acid Natural products C=1C=C(O)C(O)=CC=1C=CC(=O)OC(C(O)=O)C(C(=O)O)OC(=O)C=CC1=CC=C(O)C(O)=C1 YDDGKXBLOXEEMN-UHFFFAOYSA-N 0.000 abstract description 4
- YDDGKXBLOXEEMN-IABMMNSOSA-N chicoric acid Chemical compound O([C@@H](C(=O)O)[C@@H](OC(=O)\C=C\C=1C=C(O)C(O)=CC=1)C(O)=O)C(=O)\C=C\C1=CC=C(O)C(O)=C1 YDDGKXBLOXEEMN-IABMMNSOSA-N 0.000 abstract description 4
- 229930016920 cichoric acid Natural products 0.000 abstract description 4
- YDDGKXBLOXEEMN-WOJBJXKFSA-N dicaffeoyl-L-tartaric acid Natural products O([C@@H](C(=O)O)[C@@H](OC(=O)C=CC=1C=C(O)C(O)=CC=1)C(O)=O)C(=O)C=CC1=CC=C(O)C(O)=C1 YDDGKXBLOXEEMN-WOJBJXKFSA-N 0.000 abstract description 4
- YDDGKXBLOXEEMN-IABMMNSOSA-L Chicoric acid Natural products C1=C(O)C(O)=CC=C1\C=C\C(=O)O[C@@H](C([O-])=O)[C@H](C([O-])=O)OC(=O)\C=C\C1=CC=C(O)C(O)=C1 YDDGKXBLOXEEMN-IABMMNSOSA-L 0.000 abstract description 3
- YDDGKXBLOXEEMN-PMACEKPBSA-N dicaffeoyl-D-tartaric acid Natural products O([C@H](C(=O)O)[C@H](OC(=O)C=CC=1C=C(O)C(O)=CC=1)C(O)=O)C(=O)C=CC1=CC=C(O)C(O)=C1 YDDGKXBLOXEEMN-PMACEKPBSA-N 0.000 abstract description 3
- 238000002156 mixing Methods 0.000 abstract 1
- QAIPRVGONGVQAS-DUXPYHPUSA-N trans-caffeic acid Chemical compound OC(=O)\C=C\C1=CC=C(O)C(O)=C1 QAIPRVGONGVQAS-DUXPYHPUSA-N 0.000 description 6
- 239000000047 product Substances 0.000 description 4
- ACEAELOMUCBPJP-UHFFFAOYSA-N (E)-3,4,5-trihydroxycinnamic acid Natural products OC(=O)C=CC1=CC(O)=C(O)C(O)=C1 ACEAELOMUCBPJP-UHFFFAOYSA-N 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 3
- 235000004883 caffeic acid Nutrition 0.000 description 3
- 229940074360 caffeic acid Drugs 0.000 description 3
- QAIPRVGONGVQAS-UHFFFAOYSA-N cis-caffeic acid Natural products OC(=O)C=CC1=CC=C(O)C(O)=C1 QAIPRVGONGVQAS-UHFFFAOYSA-N 0.000 description 3
- 239000000470 constituent Substances 0.000 description 3
- 229930014626 natural product Natural products 0.000 description 3
- 241000723353 Chrysanthemum Species 0.000 description 2
- 235000007516 Chrysanthemum Nutrition 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 241001597008 Nomeidae Species 0.000 description 2
- 244000172533 Viola sororia Species 0.000 description 2
- 230000001476 alcoholic effect Effects 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000003643 water by type Substances 0.000 description 2
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 241001579678 Panthea coenobita Species 0.000 description 1
- 241001529246 Platymiscium Species 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 235000006193 cichoric acid Nutrition 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000009509 drug development Methods 0.000 description 1
- NJYVDFDTLLZVMG-UHFFFAOYSA-N echinacoside Natural products CC1OC(OC2C(O)C(OCCc3ccc(O)c(O)c3)OC(COC4OC(CO)C(O)C(O)C4O)C2OC(=O)C=Cc5cc(O)cc(O)c5)C(O)C(O)C1O NJYVDFDTLLZVMG-UHFFFAOYSA-N 0.000 description 1
- FSBUXLDOLNLABB-ISAKITKMSA-N echinacoside Chemical compound O[C@@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@H]1O[C@H]1[C@H](OC(=O)\C=C\C=2C=C(O)C(O)=CC=2)[C@@H](CO[C@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)O)O[C@@H](OCCC=2C=C(O)C(O)=CC=2)[C@@H]1O FSBUXLDOLNLABB-ISAKITKMSA-N 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- -1 filter Substances 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
- 150000002338 glycosides Chemical class 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 239000002955 immunomodulating agent Substances 0.000 description 1
- 230000002584 immunomodulator Effects 0.000 description 1
- 229940121354 immunomodulator Drugs 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 230000032696 parturition Effects 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to a method for preparing echinacea purpurea extracts. The method for preparing echinacea purpurea extracts comprises the following steps of: extracting superfine ground echinacea purpurea by adding 40 to 80 percent hydrous ethanol, and filtering to obtain filtrate and residue; and extracting the residue by adding 40 to 80 percent hydrous ethanol, filtering the solution to obtain filtrate, mixing the filtrate obtained from twice filtrations, discarding the residue, concentrating the filtrate to obtain extracturm, and vacuum drying the extracturm to obtain the echinacea purpurea extracts. The echinacea purpurea of the echinacea purpurea extracts is mainly measured by chicoric acid, and the preparation method is simple.
Description
Technical field
The present invention relates to a kind of plant extract and preparation method thereof, particularly a kind of Echinacea extract preparation method belongs to medical technical field.
Background technology
Echinacea (Echinacea) is a kind of Echinacea plant that originates in North America and Canada south, this platymiscium has 8 kinds and several mutation, present 3 kinds that mainly contain: Echinacea purpurea Moench (Echinacea purpurea as drug development, be usually said Echinacea), Echinacea angustifolia (Echinacea augustifolia) and white Echinacea (Echinacen pallida).At present, wild amount seldom, they originate in the North America, now Europe and other are regional all an introducing and planting.Ground such as China Beijing, Nanjing, Shanghai and Changsha are also introduced a fine variety and are carried out the GAP kind and plant.The main chemical compositions that Echinacea, Echinacea angustifolia and violet ash awl chrysanthemum contain can be divided into several big classes: polysaccharide and glycoprotein, polar caffeic acid derivant (caffeic acid derivatives), fat-soluble alkylamide (alkamides).Every constituents that different plants contain has difference, and the contained chemical compound of every constituents also is not quite similar.For example, the polarity part that leaches at ethanol mainly contains the caffeic acid derivant, Echinacea angustifolia and violet ash awl chrysanthemum mainly contain Echinacea glycoside (echinacoside), and Echinacea then mainly contains chicoric acid (cichoric acid), can be used as immunomodulator and stimulant.
After the China joined WTO, the Green Trade Barrier of agricultural product frequently occurs, and antibiotic residual quantity exceeds standard and having a strong impact on the international trade of animal products.In addition, in process of production there is high pollution in environment, under the trend that nowadays mankind go back to nature, has the extremely people's favor of active natural product, states such as America and Europe, Japan have strengthened the research to the natural activity product again, China has abundant animal and plant resource, the history in existing several thousand of the application of Chinese herbal medicine.The utilization modern science and technology are studied the active component of natural product, the research that comprises separation, purification, structure determination, structure and function etc. can promote human health, be the trend of new century natural product research, and for the research of Echinacea extract one of them pith especially.In feedstuff, add Echinacea, can strengthen the poultry Abwehrkraft des Koepers, reduce antibiotic use amount, can strengthen the competitiveness in the international market of exported product.But at present, for studies show that of Echinacea immunological enhancement, the immunocompetence of Echinacea has more than a kind of separate constituent and provides, but provide by multiple composition synergism, therefore, it is particularly important that the extracting method of genus echinacea plant becomes, the method difference, and all kinds of content of effective are just different in the extract.Given this, the preparation technology of further investigation and optimization Echinacea extract has great science and more practical value to the content that improves Echinacea extract.
Summary of the invention
The preparation method that the purpose of this invention is to provide a kind of Echinacea extract.
At first, the invention provides a kind of Echinacea extract, it is characterized in that it being to be prepared from by following method:
1. extract the Echinacea medical material with 40～80% aquiferous ethanol, filter, filtrate and medicinal residues;
2. the aquiferous ethanol of medicinal residues reuse 40～80% extracts, and filters, and gets filtrate and medicinal residues;
3. combining step 1 and 2 gained filtrates, concentrate extractum, vacuum drying, Echinacea extract.
Wherein, described Echinacea crude drug source is in genus echinacea plant Echinacea Echinacea purpurea, Echinacea angustifolia Echinacea augustifolia and white Echinacea Echinacen pallida.
Further, Echinacea extract of the present invention is characterized in that being prepared from through the following step:
1. get Echinacea, pulverize, the alcohol heating reflux that adds 10～20 times of amounts 40～80% extracted 1～2 hour, filtered, and collected
2. the alcohol heating reflux of the medicinal residues in the step 1 with 10～20 times of amounts 40～80% extracted 1～2 hour, filter, collect filtrate;
3. the filtrate of gained in the combining step 1 and 2, concentrating under reduced pressure gets extractum, and vacuum drying promptly gets Echinacea extract.
Further, Echinacea extract of the present invention is characterized in that giving birth to through the following step preparation:
1. get Echinacea and add 18 times of amount alcohol heating reflux of 80% and extracted 1 hour, filter, collect filtrate;
2. step 1 Chinese medicine slag was extracted 1 hour with 18 times of amount 80% alcohol heating reflux, filter, collect filtrate;
3. gained filtrate in the combining step 1 and 2, the extractum of concentrating under reduced pressure, vacuum drying promptly gets Echinacea extract.
Beneficial effect of the present invention:
1. compare main effective ingredient (chicoric acid) content height during extracting method of the present invention is extract obtained with Echinacea extract in the prior art;
2. the preparation technology of Echinacea extract of the present invention is simple, is suitable for suitability for industrialized production.
The specific embodiment
Specific implementation process of the present invention is as follows:
1. use the ethanol water heating and refluxing extraction: 10～20 times of amount ethanol waters of 40～80% are mixed with the Echinacea medical material, 60～90 ℃ of following reflux, extract, 1～2 hour, filtration, collection filtrate and medicinal residues.The ethanol waters of medicinal residues with 10～20 times of amount 40-80% are mixed, reflux, extract, 1～2 hour again under 60～90 ℃, filtration merges filtrate twice;
2. the preparation of concentrated solution: 40～60 ℃ of rotation evaporation and concentration down, concentrating under reduced pressure gets extractum with the filtrate of above-mentioned merging, then in 40～60 ℃ of following vacuum dryings, get final product Echinacea extract.
Embodiment
With Echinacea medical material 15 grams is raw material, be positioned in the round-bottomed flask, add 15 times of (being 225ml) 80% alcoholic solution, place electrical heating to put, remained under the slight boiling condition reflux, extract, 1 hour, after the filtration, collect filtrate and medicinal residues, add 15 times of amount 80% alcoholic solution again in medicinal residues, 1 hour after-filtration of reflux, extract, merges filtrate twice under the same conditions.Place Rotary Evaporators to carry out concentrating under reduced pressure filtrate, get extractum, extractum places vacuum drying oven behind the centrifugal 15min of the rotating speed of 10000r/min, and 60 ℃ of following vacuum dryings promptly get Echinacea extract.
Claims (4)
1. an Echinacea extract is characterized in that, it is prepared from by following method:
1.1 the aquiferous ethanol with 40～80% extracts the Echinacea medical material, filters, and gets filtrate and medicinal residues;
1.2 the aquiferous ethanol of medicinal residues reuse 40～80% extracts, and filters, and gets filtrate and medicinal residues;
1.3 discard final medicinal residues, merge the filtrate of extracted twice, concentrate extractum, vacuum drying, Echinacea extract
2. Echinacea extract according to claim 1, it is characterized in that: described Echinacea crude drug source is in genus echinacea plant Echinacea Echinacea purpurea, Echinacea angustifolia Echinacea augustifolia and white Echinacea Echinacen pallida.
3. Echinacea extract according to claim 1 and 2 is characterized in that, it is to be prepared from through the following step:
Add 10～20 times of amount alcohol heating reflux of 40～80% and extracted 1～2 hour 3.1 get Echinacea, filter, collect filtrate;
3.2 the alcohol heating reflux of the medicinal residues in the step 3.1 with 10～20 times of amounts 40～80% extracted 1～2 hour, filter, collect filtrate;
3.3 the filtrate of gained in combining step 3.1 and 3.2, concentrating under reduced pressure gets extractum, and vacuum drying promptly gets Echinacea extract.
4. Echinacea extract according to claim 3 is characterized in that being prepared from through the following step:
Add 18 times of amount alcohol heating reflux of 80% and extracted 1 hour 4.1 get Echinacea, filter, collect filtrate;
4.2 step 4.1 Chinese medicine slag was measured 80% ethanol extractions 1 hour with 18 times, filter, collect filtrate;
4.3 gained filtrate in combining step 4.1 and 4.2, concentrating under reduced pressure gets extractum, and vacuum drying promptly gets Echinacea extract.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910255686 CN102100718A (en) | 2009-12-16 | 2009-12-16 | Process for preparing echinacea purpurea extracts |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910255686 CN102100718A (en) | 2009-12-16 | 2009-12-16 | Process for preparing echinacea purpurea extracts |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102100718A true CN102100718A (en) | 2011-06-22 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200910255686 Pending CN102100718A (en) | 2009-12-16 | 2009-12-16 | Process for preparing echinacea purpurea extracts |
Country Status (1)
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| CN (1) | CN102100718A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107375377A (en) * | 2017-07-14 | 2017-11-24 | 华南农业大学 | A kind of livestock and poultry echinacea root Chinese medical extract and preparation method and application |
| CN115886126A (en) * | 2022-10-17 | 2023-04-04 | 青海大学 | Chichoric acid feed additive, preparation method and application based on plateau environment |
-
2009
- 2009-12-16 CN CN 200910255686 patent/CN102100718A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107375377A (en) * | 2017-07-14 | 2017-11-24 | 华南农业大学 | A kind of livestock and poultry echinacea root Chinese medical extract and preparation method and application |
| CN115886126A (en) * | 2022-10-17 | 2023-04-04 | 青海大学 | Chichoric acid feed additive, preparation method and application based on plateau environment |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20110622 |