CN107372623A - Bitter rot or anthracnose of grape vaccine - Google Patents

Bitter rot or anthracnose of grape vaccine Download PDF

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Publication number
CN107372623A
CN107372623A CN201710645238.3A CN201710645238A CN107372623A CN 107372623 A CN107372623 A CN 107372623A CN 201710645238 A CN201710645238 A CN 201710645238A CN 107372623 A CN107372623 A CN 107372623A
Authority
CN
China
Prior art keywords
vaccine
grape
anthracnose
bitter rot
extraction
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201710645238.3A
Other languages
Chinese (zh)
Inventor
柏玉兰
苏轩
郭峰
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Henan Bo Yu Plant Immune Technology Co Ltd
Original Assignee
Henan Bo Yu Plant Immune Technology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Henan Bo Yu Plant Immune Technology Co Ltd filed Critical Henan Bo Yu Plant Immune Technology Co Ltd
Priority to CN201710645238.3A priority Critical patent/CN107372623A/en
Publication of CN107372623A publication Critical patent/CN107372623A/en
Pending legal-status Critical Current

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Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08Magnoliopsida [dicotyledons]

Abstract

A kind of bitter rot or anthracnose of grape vaccine, it is characterized in that:Include Noni fruit, ethanol extraction.The beneficial effects of the invention are as follows:Plant extracts, so can preferably cause plant absorption, and obtained for the simple extraction of values of single plant, operation is extremely simple, without relying on excessively complicated genetic engineering equipment;And this extract have greatly expanded the broad spectrum activity of vaccine not only for anthrax-bacilus, and for other fungies also by preferably killing control action.

Description

Bitter rot or anthracnose of grape vaccine
Technical field
The invention belongs to a kind of grape vaccine.
Background technology
Existing grape vaccine, using active peptide and inhibiting factor, protein content is higher, is unfavorable for plant for component Directly absorb, it is necessary to which equipment and the operation of genetic engineering, operation are complex and cumbersome.
The content of the invention
In order to solve the above problems, the present invention provides a kind of bitter rot or anthracnose of grape vaccine, there is killing control action to anthrax-bacilus, Plant absorption can preferably be caused, and obtained for the simple extraction of values of single plant, operation is extremely simple, excessively complicated without relying on Genetic engineering equipment.
To achieve these goals, the technical solution adopted by the present invention is:A kind of bitter rot or anthracnose of grape vaccine, it is characterized in that: Include Noni fruit, ethanol extraction.
Wherein, described bitter rot or anthracnose of grape vaccine is cream solid.
The beneficial effects of the invention are as follows:Plant extracts, so can preferably cause plant absorption, and it is single plant Simple extraction of values obtains, and operation is extremely simple, without relying on excessively complicated genetic engineering equipment;And this extract is not only for charcoal Subcutaneous ulcer bacterium, and have greatly expanded the broad spectrum activity of vaccine also by preferably killing control action for other fungies.
The preparation method of bitter rot or anthracnose of grape vaccine is:
Step 1, raw material Noni fruit is got out;
Step 2, squeezed the juice, after squeezing the juice, centrifuged in the centrifuge with 4 layers of gauze, control rotating speed 4800r/ Min, it is 12min to control the reaction time;
Step 3, then the liquid of centrifugation and mass fraction are mixed for 90% ethanol, while applies the ultrasound of 30Khz intensity Ripple, substantially 28 DEG C, substantially 46min of each time of each reaction temperature is controlled, so extraction four times, obtain extract
Step 4, after extract is mixed, concentration is paste in evaporimeter, and then low temperature is done under the conditions of temperature is -10 degree It is dry, produce product.
Brief description of the drawings
Fig. 1 is a kind of flow chart of bitter rot or anthracnose of grape vaccine production method.
Embodiment
To achieve the above object, a kind of technical scheme of bitter rot or anthracnose of grape vaccine of the present invention is:
Bitter rot or anthracnose of grape vaccine, it is characterized in that, include Noni fruit, ethanol extraction.
Wherein, described bitter rot or anthracnose of grape vaccine is cream solid.
The preparation method of bitter rot or anthracnose of grape vaccine is:
Step 1, raw material Noni fruit is got out;
Step 2, squeezed the juice, after squeezing the juice, centrifuged in the centrifuge with 4 layers of gauze, control rotating speed 4800r/ Min, it is 12min to control the reaction time;
Step 3, then the liquid of centrifugation and mass fraction are mixed for 90% ethanol, while applies the ultrasound of 30Khz intensity Ripple, substantially 28 DEG C, substantially 46min of each time of each reaction temperature is controlled, so extraction four times, obtain extract
Step 4, after extract is mixed, concentration is paste in evaporimeter, and then low temperature is done under the conditions of temperature is -10 degree It is dry, produce product.

Claims (3)

1. a kind of bitter rot or anthracnose of grape vaccine, it is characterized in that:Include Noni fruit, ethanol extraction.
2. a kind of bitter rot or anthracnose of grape vaccine according to claim 1 is cream solid.
3. the preparation method of bitter rot or anthracnose of grape vaccine, it is characterized in that:
Step 1, raw material Noni fruit is got out;
Step 2, squeezed the juice, after squeezing the juice, centrifuged in the centrifuge with 4 layers of gauze, control rotating speed 4800r/ Min, it is 12min to control the reaction time;
Step 3, then the liquid of centrifugation and mass fraction are mixed for 90% ethanol, while applies the ultrasound of 30Khz intensity Ripple, substantially 28 DEG C, substantially 46min of each time of each reaction temperature is controlled, so extraction four times, obtain extract;
Step 4, after extract is mixed, concentration is paste in evaporimeter, and then low temperature is done under the conditions of temperature is -10 degree It is dry, produce product.
CN201710645238.3A 2017-08-01 2017-08-01 Bitter rot or anthracnose of grape vaccine Pending CN107372623A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710645238.3A CN107372623A (en) 2017-08-01 2017-08-01 Bitter rot or anthracnose of grape vaccine

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201710645238.3A CN107372623A (en) 2017-08-01 2017-08-01 Bitter rot or anthracnose of grape vaccine

Publications (1)

Publication Number Publication Date
CN107372623A true CN107372623A (en) 2017-11-24

Family

ID=60343242

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201710645238.3A Pending CN107372623A (en) 2017-08-01 2017-08-01 Bitter rot or anthracnose of grape vaccine

Country Status (1)

Country Link
CN (1) CN107372623A (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015214508A (en) * 2014-05-09 2015-12-03 Meiji Seikaファルマ株式会社 Resistance inducer and compound
CN105145643A (en) * 2015-08-07 2015-12-16 中国热带农业科学院海口实验站 Morinda citrifolia extract and application thereof to preparation of drugs for controlling plant anthracnose
CN106614820A (en) * 2016-11-24 2017-05-10 蚌埠市宗洼草鸡养殖农民专业合作社 Composition for preventing and treating anthracnose of spinach

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015214508A (en) * 2014-05-09 2015-12-03 Meiji Seikaファルマ株式会社 Resistance inducer and compound
CN105145643A (en) * 2015-08-07 2015-12-16 中国热带农业科学院海口实验站 Morinda citrifolia extract and application thereof to preparation of drugs for controlling plant anthracnose
CN106614820A (en) * 2016-11-24 2017-05-10 蚌埠市宗洼草鸡养殖农民专业合作社 Composition for preventing and treating anthracnose of spinach

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
张素敏 等: "《园林植物病害发生与防治》", 30 September 2014, 中国农业大学出版社 *

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Application publication date: 20171124