CN107372102A - A kind of selection of anti-SPVD virosis sweet potato variety - Google Patents
A kind of selection of anti-SPVD virosis sweet potato variety Download PDFInfo
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- CN107372102A CN107372102A CN201710661599.7A CN201710661599A CN107372102A CN 107372102 A CN107372102 A CN 107372102A CN 201710661599 A CN201710661599 A CN 201710661599A CN 107372102 A CN107372102 A CN 107372102A
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- 244000017020 Ipomoea batatas Species 0.000 title claims abstract description 26
- 235000002678 Ipomoea batatas Nutrition 0.000 title claims abstract description 26
- 208000036142 Viral infection Diseases 0.000 title claims abstract description 12
- 241000700605 Viruses Species 0.000 claims abstract description 25
- 238000004519 manufacturing process Methods 0.000 claims abstract description 19
- 230000003612 virological effect Effects 0.000 claims abstract description 18
- 241001124076 Aphididae Species 0.000 claims abstract description 15
- 239000002574 poison Substances 0.000 claims abstract description 10
- 231100000614 poison Toxicity 0.000 claims abstract description 10
- 238000012216 screening Methods 0.000 claims abstract description 10
- 208000024891 symptom Diseases 0.000 claims abstract description 7
- 230000000840 anti-viral effect Effects 0.000 claims abstract description 6
- 241000196324 Embryophyta Species 0.000 claims description 51
- 201000010099 disease Diseases 0.000 claims description 50
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 50
- 238000003306 harvesting Methods 0.000 claims description 10
- 238000010899 nucleation Methods 0.000 claims description 9
- 238000003556 assay Methods 0.000 claims description 6
- 238000003860 storage Methods 0.000 claims description 6
- 241000217377 Amblema plicata Species 0.000 claims description 3
- 241000238631 Hexapoda Species 0.000 claims description 3
- 238000011081 inoculation Methods 0.000 claims description 3
- 108090000623 proteins and genes Proteins 0.000 claims description 3
- 238000009395 breeding Methods 0.000 abstract description 9
- 230000001488 breeding effect Effects 0.000 abstract description 8
- 241000725680 Sweet potato feathery mottle virus Species 0.000 abstract description 4
- 208000035240 Disease Resistance Diseases 0.000 abstract description 2
- 241001609660 Sweet potato chlorotic stunt virus Species 0.000 abstract 1
- 238000000034 method Methods 0.000 description 2
- 240000006153 Dioscorea trifida Species 0.000 description 1
- 235000002718 Dioscorea trifida Nutrition 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 230000000908 anti-sweet Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000000686 essence Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/18—Testing for antimicrobial activity of a material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
Abstract
The present invention discloses a kind of selection of anti-SPVD virosis sweet potato variety, belongs to agriculture breeding for disease resistance field, comprises the following steps：1) selection of seedling；2) high-voltage connecting of seedling is malicious under the conditions of solarium；3) selection of high pressure again of F1 generation；4) final election garden symptom and yields screening, and antiviral type identification；5) garden multiple spot pilot production is identified.The principle that the present invention is occurred according to sweet potato SPVD by two kinds of virus cooperations of SPFMV and SPCSV, the propagation viral according to two kinds is mainly by the condition of aphid and aleyrodid, set and identify garden multiple spot pilot production as base program using the selection of high pressure again → final election garden symptom and yields screening and resistance to Virus Type are identified → of high-voltage connecting poison → F1 generation of seedling under the conditions of solarium, the resistance to viral kind that can be applied in production is cultivated, effectively controls SPVD virosis.
Description
Technical field
The present invention relates to a kind of selection of anti-SPVD virosis sweet potato variety, belong to agriculture breeding for disease resistance field.
Background technology
Sweet potato SPVD virosis is to move back green stunt virus (SPCSV) altogether by Sweet Potato Feathery Mottle Virus (SPFMV) and sweet potato
Cause with synergism, aphid and aleyrodid are mainly leaned in two kinds of viral propagation, and sweet potato moves back green dwarfing toxogen and occurred sternly in Africa
Weight, reported in first Chinese within 2009, there is generation in three yampi areas of the whole nation now, and production loss is big, and the general underproduction is in 46-
80%, serious even has no harvest.Found that the state of an illness aggravated year by year first from 2009, it has also become the disease of the upper most serious of sweet potato production
One of evil.
The seed selection and popularization of disease-resistant variety are to control the Main Means of the disease.But there is presently no on anti-SPVD kinds
The report of selection, what the seed selections of anti-sweet potato SPVD kinds followed substantially is natural selection rule, establishes anti-SPVD diseases as early as possible
The seed selection system of malicious sweet potato variety, using its disease occurrence characteristic, in the selection of existing sweet potato variety, add high-voltage connecting
The step of poison and high pressure selection, meanwhile, using the resistance to malicious type of engrafting method identification of species, foundation is efficient, fast and accurately resists
Sweet potato SPVD breed breeding methods, the resistance to viral kind that can be applied in production is cultivated, is the task of top priority to work at present.
The content of the invention
In view of the above-mentioned problems of the prior art, the present invention provides a kind of seed selection side of anti-SPVD virosis sweet potato variety
Method, the resistance to viral kind that can be applied in production is cultivated, effectively control SPVD virosis.
To achieve these goals, the selection for a kind of anti-SPVD virosis sweet potato variety that the present invention uses, specifically
Comprise the following steps：
1) selection of seedling：
Have in selection Parent and show more resistance to kind offspring under field conditions (factors), true seed is sowed, cultivate
It is standby after being grown to 20cm；
2) high-voltage connecting of seedling is malicious under the conditions of solarium：
Step a, micro-ridge is played in 60 mesh solariums, row spacing 75cm, ready seedling is planted in solarium, density
4500 plants/acre；
Step b, by the aphid and aleyrodid of ready band SPVD viruses, and the aobvious disease strain of potted plant SPVD viruses, put simultaneously
Enter in solarium, in 300m2Greenhouse in, put two basins every three ridges, the inoculation aphid and each 200 of aleyrodid per basin；
Step c, closed solarium, while hot and humid environment is made, cultivate aphid and aleyrodid carries out effective insect and connects poison；
Step d, handled by 120 days, all seedlings are subjected to individual plant harvest, F1 generation is designated as, is put into sweet potato storage cellar
In；
3) selection of high pressure again of F1 generation：
Step a, the F1 generation seedling individual plant in cellar for storing things is taken out and carries out normal seeding by Second Year, and SPVD is constantly removed after emergence
Aobvious disease strain, all aobvious disease systems are eliminated, until carrying out second of solarium's plantation；
Step b, play micro-ridge in 60 mesh solariums, row spacing 75cm, the non-aobvious disease system that will be left behind, be 5 plants with every and plant in net
In room, then aphid and aleyrodid by ready band SPVD viruses, and the aobvious disease strain of potted plant SPVD viruses, it is put into solarium, it is close
Degree is same as above；
Step c, in 120 day growth period, routine observation is each that SPVD shows disease situation, eliminates aobvious disease system in time；
Step d, after 120 days, it is to carry out harvest storage to divide；
4) final election garden symptom and yields screening, and antiviral type identification：
Step a, carry out normal seeding within the 3rd year, continue to eliminate all aobvious diseases systems, carry out field two and repeat, often repeatedly 10 plants
Cell identification；Aobvious disease strain is constantly eliminated in whole growth period, retains the two non-aobvious disease systems for repeating to show disease plant without SPVD,
Harvested and stored after 120 days；
Step b, while the identification of final election garden is carried out, potted plant grafting SPVD Viral Assays are carried out to all strains, to each
The resistance to Virus Type of strain is evaluated；
The specific steps of the potted plant grafting SPVD Viral Assays, including identification strain is planted in basin, per potted plant kind 3
Strain, each strain plant 4 basins, ready SPVD severes strain are grafted onto on each plant, per the basin of strain grafting 3, retain 1 basin and do
Control, the SPVD for starting to observe each plant for 30 days after grafting show disease situation, investigated within 60 days, and aobvious disease strain belongs to anti-insect and passes poison
Type, the non-aobvious resistance to malicious type of disease strain category gene；
5) garden multiple spot pilot production is identified：
Normal seeding was carried out to screening in final election garden in 4th year, carry out more ground field three and repeat, often repeatedly 60 plants, meanwhile,
The detoxic seedling of local main breed is set as check plot, in harvest, determines each cell production, as yield is not less than when landlord plants
The detoxic seedling cell production of kind, it can be assumed that the strain is resistance to viral strain, and the executive pipe portion door of higher level is submitted to be recognized
It is fixed.
The principle that the present invention is occurred according to sweet potato SPVD by two kinds of virus cooperations of SPFMV and SPCSV, viral according to two kinds
The condition mainly by aphid and aleyrodid is propagated, is set with the sub-high pressure again of high-voltage connecting poison → F1 generation of seedling under the conditions of solarium
It is base program to identify garden multiple spot pilot production for selection → final election garden symptom and yields screening and resistance to Virus Type are identified →, training
The resistance to viral kind that can be applied in production is brought out, effectively controls SPVD virosis.
Brief description of the drawings
Fig. 1 is the seed selection flow chart of the present invention.
Embodiment
To make the object, technical solutions and advantages of the present invention of greater clarity, below by drawings and Examples, to this
Invention is further elaborated.However, it should be understood that the specific embodiments described herein are merely illustrative of the present invention,
The scope being not intended to limit the invention.
Unless otherwise defined, all technical terms and scientific terminology used herein are led with belonging to the technology of the present invention
The implication that the technical staff in domain is generally understood that is identical, and used term is intended merely to retouch in the description of the invention herein
State the purpose of specific embodiment, it is not intended that in the limitation present invention.
As shown in figure 1, a kind of selection of anti-SPVD virosis sweet potato variety, specifically includes following steps：
1) selection of seedling：
Have in selection Parent and show more resistance to kind offspring under field conditions (factors), true seed is sowed, cultivate
It is standby after being grown to 20cm；
2) high-voltage connecting of seedling is malicious under the conditions of solarium：
Step a, micro-ridge is played in 60 mesh solariums, row spacing 75cm, ready seedling is planted in solarium, density
4500 plants/acre；
Step b, by the aphid and aleyrodid of ready band SPVD viruses, and the aobvious disease strain of potted plant SPVD viruses, put simultaneously
Enter in solarium, in 300m2Greenhouse in, put two basins every three ridges, the inoculation aphid and each 200 of aleyrodid per basin；
Step c, closed solarium, while hot and humid environment is made, cultivate aphid and aleyrodid carries out effective insect and connects poison；
Step d, handled by 120 days, all seedlings are subjected to individual plant harvest, F1 generation is designated as, is put into sweet potato storage cellar
In；
3) selection of high pressure again of F1 generation：
Step a, the F1 generation seedling individual plant in cellar for storing things is taken out and carries out normal seeding by Second Year, and SPVD is constantly removed after emergence
Aobvious disease strain, all aobvious disease systems are eliminated, until carrying out second of solarium's plantation；
Step b, play micro-ridge in 60 mesh solariums, row spacing 75cm, the non-aobvious disease system that will be left behind, be 5 plants with every and plant in net
In room, then aphid and aleyrodid by ready band SPVD viruses, and the aobvious disease strain of potted plant SPVD viruses, it is put into solarium, it is close
Degree is same as above；
Step c, in 120 day growth period, routine observation is each that SPVD shows disease situation, eliminates aobvious disease system in time；
Step d, after 120 days, it is to carry out harvest storage to divide；
4) final election garden symptom and yields screening, and antiviral type identification：
Step a, carry out normal seeding within the 3rd year, continue to eliminate all aobvious diseases systems, carry out field two and repeat, often repeatedly 10 plants
Cell identification；Aobvious disease strain is constantly eliminated in whole growth period, retains the two non-aobvious disease systems for repeating to show disease plant without SPVD,
Harvested and stored after 120 days；
Step b, while the identification of final election garden is carried out, potted plant grafting SPVD Viral Assays are carried out to all strains, to each
The resistance to Virus Type of strain is evaluated；
The specific steps of the potted plant grafting SPVD Viral Assays, including identification strain is planted in basin, per potted plant kind 3
Strain, each strain plant 4 basins, ready SPVD severes strain are grafted onto on each plant, per the basin of strain grafting 3, retain 1 basin and do
Control, the SPVD for starting to observe each plant for 30 days after grafting show disease situation, investigated within 60 days, and aobvious disease strain belongs to anti-insect and passes poison
Type, the non-aobvious resistance to malicious type of disease strain category gene；
5) garden multiple spot pilot production is identified：
Normal seeding was carried out to screening in final election garden in 4th year, carry out more ground field three and repeat, often repeatedly 60 plants, meanwhile,
The detoxic seedling of local main breed is set as check plot, in harvest, determines each cell production, as yield is not less than when landlord plants
The detoxic seedling cell production of kind, it can be assumed that the strain is resistance to viral strain, and the executive pipe portion door of higher level is submitted to be recognized
It is fixed.
Embodiment 1
3 years antiviral breed breedings are carried out continuously in, in 2012 in 2013 in 2014, as described above anti-SPVD diseases
The selection of viral disease sweet potato variety carries out breed breeding：By seedling under the conditions of solarium high-voltage connecting poison → F1 generation again
The real for many years of garden multiple spot pilot production is identified in high pressure selection → final election garden symptom and yields screening and resistance to Virus Type are identified →
Trample, obtained anti-(resistance to) virus strain on a small quantity.
Each time respectively incorporates into selection condition, respectively as shown in table 1, table 2 and table 3.
1 2012 years Sweet Potato Against SPVD breed breeding summary sheets of table
2 2013 years Sweet Potato Against SPVD breed breeding summary sheets of table
3 2014 years Sweet Potato Against SPVD breed breeding summary sheets of table
Collect knot according to from the 2012-2014 seed selections to 44 combinations, 546 anti-SPVD viruses kinds for being in continuous 3 years
Fruit shows：Selected by 2 years high pressures, the virus pulled out shows disease system：2012-2014 (91.84%), 2013-2015
(74.81%), 2014-2016 (61.25%), the antiviral strain of reservation are：2012-2014 (8.16%), 2013-2015
(25.19%), 2014-2016 (38.75%), Sweet Potato Against (resistance to) virus strain is effectively obtained in a short time, is improved anti-
The efficiency of selection of viral kind and the variety selection pressure of conventional breeding programmes.
As can be seen here, the seed selection of Sweet Potato Against SPVD kinds can be quickly and efficiently carried out using the selection of the present invention.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
Any modification, equivalent substitution or improvement made within refreshing and principle etc., should be included in the scope of the protection.
Claims (1)
1. a kind of selection of anti-SPVD virosis sweet potato variety, it is characterised in that specifically include following steps：
1) selection of seedling：
Have in selection Parent and show more resistance to kind offspring under field conditions (factors), true seed is sowed, culture is arrived
It is standby after 20cm length；
2) high-voltage connecting of seedling is malicious under the conditions of solarium：
Step a, micro-ridge is played in 60 mesh solariums, row spacing 75cm, ready seedling is planted in solarium, density 4500
Plant/acre；
Step b, by the aphid and aleyrodid of ready band SPVD viruses, and the aobvious disease strain of potted plant SPVD viruses, while it is put into net
In room, in 300m2Greenhouse in, put two basins every three ridges, the inoculation aphid and each 200 of aleyrodid per basin；
Step c, closed solarium, while hot and humid environment is made, cultivate aphid and aleyrodid carries out effective insect and connects poison；
Step d, handled by 120 days, all seedlings are subjected to individual plant harvest, F1 generation is designated as, is put into sweet potato storage cellar；
3) selection of high pressure again of F1 generation：
Step a, the F1 generation seedling individual plant in cellar for storing things is taken out and carries out normal seeding by Second Year, and SPVD is constantly removed after emergence and shows disease
Strain, all aobvious disease systems are eliminated, until carrying out second of solarium's plantation；
Step b, play micro-ridge in 60 mesh solariums, row spacing 75cm, the non-aobvious disease system that will be left behind, be 5 plants with every and plant in solarium,
Again by the aphid and aleyrodid of ready band SPVD viruses, and the aobvious disease strain of potted plant SPVD viruses, it is put into solarium, density is same
On；
Step c, in 120 day growth period, routine observation is each that SPVD shows disease situation, eliminates aobvious disease system in time；
Step d, after 120 days, it is to carry out harvest storage to divide；
4) final election garden symptom and yields screening, and antiviral type identification：
Step a, carry out normal seeding within the 3rd year, continue to eliminate all aobvious diseases systems, carry out field two and repeat, often repeatedly 10 plants small
Identify in area；Show disease strain in continuous eliminate of whole growth period, it is 120 to retain two repetitions without the SPVD non-aobvious diseases for showing disease plant
Harvested and stored after it；
Step b, while the identification of final election garden is carried out, potted plant grafting SPVD Viral Assays are carried out to all strains, to each strain
Resistance to Virus Type evaluated；
The specific steps of the potted plant grafting SPVD Viral Assays, including identification strain is planted in basin, per potted plant 3 plants of kind,
Each strain plants 4 basins, and ready SPVD severes strain is grafted onto on each plant, per the basin of strain grafting 3, retains 1 basin and does pair
According to the SPVD for starting to observe each plant for 30 days after grafting shows disease situation, is investigated within 60 days, and aobvious disease strain belongs to anti-insect and passes poison
Type, the non-aobvious resistance to malicious type of disease strain category gene；
5) garden multiple spot pilot production is identified：
Normal seeding was carried out to screening in final election garden in 4th year, carry out more ground field three and repeat, often repeatedly 60 plants, meanwhile, setting
The detoxic seedling of local main breed is check plot, in harvest, each cell production is determined, as yield is not less than local main breed
Detoxic seedling cell production, it can be assumed that the strain is resistance to viral strain, and submit to the executive pipe portion door of higher level to be assert.
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| CN201710661599.7A CN107372102B (en) | 2017-08-04 | 2017-08-04 | A kind of selection of anti-SPVD virosis sweet potato variety |
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| CN107372102B CN107372102B (en) | 2019-05-03 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109463150A (en) * | 2018-09-27 | 2019-03-15 | 江西万雅生态旅游开发有限公司 | Booth frame for yangtao and Kiwi berry grafting assessment system and its method |
| CN110199881A (en) * | 2019-07-10 | 2019-09-06 | 江苏徐淮地区徐州农业科学研究所(江苏徐州甘薯研究中心) | A kind of sweet potato health seedling cultural method removing sweet potato viruses complex SPVD |
| CN110367123A (en) * | 2019-08-21 | 2019-10-25 | 江苏徐淮地区徐州农业科学研究所(江苏徐州甘薯研究中心) | A kind of Resistance Identification method of sweet potato leaf curl viral disease |
| CN113249403A (en) * | 2021-03-31 | 2021-08-13 | 江苏师范大学 | Breeding method of sweet potatoes with SPVD resistance |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109463150A (en) * | 2018-09-27 | 2019-03-15 | 江西万雅生态旅游开发有限公司 | Booth frame for yangtao and Kiwi berry grafting assessment system and its method |
| CN110199881A (en) * | 2019-07-10 | 2019-09-06 | 江苏徐淮地区徐州农业科学研究所(江苏徐州甘薯研究中心) | A kind of sweet potato health seedling cultural method removing sweet potato viruses complex SPVD |
| CN110199881B (en) * | 2019-07-10 | 2022-06-07 | 江苏徐淮地区徐州农业科学研究所(江苏徐州甘薯研究中心) | Sweet potato healthy seedling culture method for removing sweet potato virus complex SPVD |
| CN110367123A (en) * | 2019-08-21 | 2019-10-25 | 江苏徐淮地区徐州农业科学研究所(江苏徐州甘薯研究中心) | A kind of Resistance Identification method of sweet potato leaf curl viral disease |
| CN113249403A (en) * | 2021-03-31 | 2021-08-13 | 江苏师范大学 | Breeding method of sweet potatoes with SPVD resistance |
| CN113249403B (en) * | 2021-03-31 | 2023-08-04 | 江苏师范大学 | Breeding method of sweet potato with SPVD resistance |
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| Publication number | Publication date |
|---|---|
| CN107372102B (en) | 2019-05-03 |
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Application publication date: 20171124 Assignee: Xuzhou Runnong Feed Co.,Ltd. Assignor: JIANGSU XUHUAI DISTRICT XUZHOU AGRICULTURAL Research Institute (JIANGSU XUZHOU SWEET POTATO RESEARCH CENTER) Contract record no.: X2023980049634 Denomination of invention: A breeding method for sweet potato varieties resistant to SPVD virus disease Granted publication date: 20190503 License type: Common License Record date: 20231205 |