CN107365215A - One kind contains diatomaceous microbe soil insecticide preparation method - Google Patents
One kind contains diatomaceous microbe soil insecticide preparation method Download PDFInfo
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- CN107365215A CN107365215A CN201610303382.4A CN201610303382A CN107365215A CN 107365215 A CN107365215 A CN 107365215A CN 201610303382 A CN201610303382 A CN 201610303382A CN 107365215 A CN107365215 A CN 107365215A
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- class
- class inoculum
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- lactobacillus plantarum
- saccharomyces cerevisiae
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Classifications
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The present invention provides one kind and contains diatomaceous microbe soil insecticide preparation method, is related to a kind of agricultural microorganism preparation technique field.The invention following steps：It is prepared by first class inoculum；It is prepared by second class inoculum；It is prepared by three-class strain；Biological organic fertilizer ferments.Contained microorganism largely breeds in soil in the biological organic fertilizer that the present invention generates, and the conversion of soil nutrient is accelerated in its propagation and metabolic process, improves utilization rate of fertilizer, while inhibit the growth of a variety of pathogens.
Description
Technical field
The present invention relates to a kind of agricultural microorganism preparation technique field, contain more particularly to one kind
Diatomaceous microbe soil insecticide preparation method.
Background technology
Chemical fertilizer, agricultural chemicals, agrochemical tillage method are excessively applied because people are irrational, makes soil
Degenerate very serious.It is mainly shown as soil hardening, salinization of soil, acidifying, element is unbalance, chemical dirt
Degeneration of dye, Organic Matter Loss and animals and plants fauna etc., seriously constrains the development of fertility.
Diatomite is a kind of siliceous rock, be mainly distributed on China, the U.S., Denmark, France, the Soviet Union,
The states such as Romania, it is a kind of biogenic siliceous sedimentary rock, it is mainly by the remains of ancient times diatom
Formed.Its chemical composition can represent there is very strong suction based on SiO2 with SiO2nH2O
It is water-based, easily to clay into power, each trickle particle of diatomite powder carries very sharp keen edge,
Insect surfaces can be attached to when being contacted with insect, scratch insect body surface, and absorb the wax of insect body surface
Layer and internal moisture, cause insect dead because of dehydration, are mechanical thing so as to reach the purpose of desinsection
Desinsection mode is managed, insect never develops immunity to drugs, and harmless to people and domestic animal.
Lactobacillus plantarum is one kind of lactic acid bacteria, is a kind of Gram-positive, does not produce gemma, anaerobism life
Long bacterium, the difference of this bacterium and other lactic acid bacteria are that the viable count of this bacterium is higher, can be substantial amounts of
Lactic acid is produced, can suppress the growth of harmful bacteria, and the acidic materials of its output can degrade in soil
Heavy metal.
Saccharomyces cerevisiae is unicellular microorganism, belongs to Mycophyta, and acid resistance is strong, is facultative anaerobic bacteria,
The oxygen in enteron aisle can be consumed and cause anaerobic environment, and be metabolized and produce the organic acids such as lactic acid, so as to favourable
In the growth of the beneficial bacteriums such as lactic acid bacteria, meanwhile, yeast is short of money well to having for multiple pathogenic microorganisms
Anti- effect, suppress various germs, anti-continuous cropping, the mitigations such as the fungi in soil, bacterium, chain knife bacterium and make
Thing growth period disease occurs.
Trichoderma harzianum belongs to Deuteromycotina, Hyphomycetes, hyphomycetales, glues spore mushroom, is a kind of universal
Existing fungi, it by producing antibiotic, hyperparasitism, bacteriolysis, Competition etc.,
Pathogenic microorganism in a variety of soil can effectively be suppressed, according to statistics, it at least belongs to 29 kinds to 18
Disease fungus is presented with antagonism in vitro or in vivo, and Trichoderma harzianum and its tunning can improve work
Thing related enzyme activity, promote absorption of the plant to nitrogen, phosphorus, potassium；Optimize the microbial environment of soil,
Promote plant growth.
The content of the invention
Weak point present in regarding to the issue above, the present invention provide one kind and contain diatomaceous micro- life
Thing soil insecticide preparation method, make microorganism contained in the biological organic fertilizer of its generation in soil
In largely breed, accelerate the conversion of soil nutrient in its propagation and metabolic process, improve fertilizer
Utilization rate, while inhibit the growth of a variety of pathogens.
In order to solve the above problems, the present invention provides one kind and contains diatomaceous microbe soil insecticide
Preparation method, wherein, comprise the following steps：
It is prepared by S10, first class inoculum；
It is prepared by S20, second class inoculum；
It is prepared by S30, three-class strain；
S40, biological organic fertilizer fermentation.
Preferably, it is further comprising the steps of in the step S10：
S101, Lactobacillus plantarum first class inoculum preparation：In the bottled fluid nutrient medium of 500ml drips
400ml, sterile access Lactobacillus plantarum test tube strains, after static gas wave refrigerator 48h it is plant at 28 DEG C
Lactobacillus first class inoculum；
S102, saccharomyces cerevisiae first class inoculum preparation：In the bottled fluid nutrient medium 400ml of 500ml drips,
Sterile access saccharomyces cerevisiae test tube strains, after static gas wave refrigerator 48h it is saccharomyces cerevisiae one-level bacterium at 28 DEG C
Kind；
S103, Trichoderma harzianum first class inoculum preparation：In the bottled fluid nutrient medium 150ml of 500ml triangles,
Sterile access Trichoderma harzianum test tube strains, are cultivated in shaking table, and condition is 28 DEG C, 120r/min is trained
It is Trichoderma harzianum first class inoculum after foster 48h.
Preferably, it is further comprising the steps of in the step S20：
S201, Lactobacillus plantarum second class inoculum preparation：In the bottled fluid nutrient medium of 500ml drips
360ml, sterile access Lactobacillus plantarum first class inoculum 40ml, at 28 DEG C after static gas wave refrigerator 48h i.e.
For Lactobacillus plantarum second class inoculum；
S202, saccharomyces cerevisiae second class inoculum preparation：In the bottled fluid nutrient medium 360ml of 500ml drips,
Sterile access saccharomyces cerevisiae first class inoculum 40ml, ferment of as being made wine after static gas wave refrigerator 48h at 28 DEG C
Female second class inoculum；
S203, Trichoderma harzianum second class inoculum preparation：In the bottled fluid nutrient medium 130ml of 500ml triangles,
Sterile access Trichoderma harzianum first class inoculum 20ml, is cultivated in shaking table, and condition is 28 DEG C, 120r/
It is Trichoderma harzianum second class inoculum after min cultures 48h.
Preferably, it is further comprising the steps of in the step S30：
S301, Lactobacillus plantarum and saccharomyces cerevisiae complex solid strain preparation：1000 kilograms of solid trainings
Support base and be inoculated with 30 kilograms of Lactobacillus plantarum second class inoculums and 30 kilograms of saccharomyces cerevisiae second class inoculums, regulation
Fermentation material water content 50% or so, at 28 DEG C sealed fermenting after 15 days be Lactobacillus plantarum and
Saccharomyces cerevisiae complex solid strain；
S302, Lactobacillus plantarum and saccharomyces cerevisiae composite fluid strain preparation：1000L Liquid Cultures
Base is inoculated with 20 kilograms of Lactobacillus plantarum second class inoculums and 20 kilograms of saccharomyces cerevisiae second class inoculums, 28
Static ferment 15 days of anaerobism is Lactobacillus plantarum and saccharomyces cerevisiae composite fluid strain at DEG C；
S303, Trichoderma harzianum three-class strain preparation：1000 kilograms of solid mediums are inoculated with 50 kilograms
Trichoderma harzianum second class inoculum, for regulation fermentation material water content 50% or so, the 5cm that tiled at 28 DEG C is shallow
Layer fermentation 7d is Trichoderma harzianum three-level solid spawn.
Preferably, in the step S40, in addition to biological organic fertilizer fermentation process and its fermentation training
Support the preparation of base：Every 1000 kilograms of organic fertilizer production fermentation mediums add 50 kilograms of Lactobacillus plantarums
Three-level composite fluid strain, 50 kilograms of Lactobacillus plantarum three-level complex solid strains, 30 kilograms of Kazakhstan thatches
Trichoderma three-class strain, regulation fermentation material water content is in 45-55%, raft fermentation, every turning in three days once,
After fermentation 15 days, the fermentate is mixed with diatomite powder by weight 4: 1 and mixed thoroughly, low temperature dries
It is 20%-30% to do to water content.
Preferably, first class inoculum is individually cultivated, and one-level second class inoculum culture medium is liquid, high temperature
Used after autoclaving.
Compared with prior art, the present invention has advantages below：
Diatomite, corn flour, wheat bran, stalk etc. are utilized Lactobacillus plantarum and saccharomycete group by the present invention
Into compound bacteria in carry out anaerobic fermentation and produce substantial amounts of organic acid and complex enzyme, and what is formed a large amount of has
Machine matter, by Trichoderma harzianum piece liquid fermentation production after spray drying, be added to Lactobacillus plantarum and
In fermentation by saccharomyces cerevisiae product, be made containing Lactobacillus plantarum, saccharomyces cerevisiae and Trichoderma harzianum viable bacteria and
The biological organic fertilizer of kieselguhr micropowder, after being applied to soil, its organic mass-energy contained promotes soil micro-
The activity of biology；Because organic matter is negatively charged, so there is the ability of stronger absorption cation；It is organic
The complexing of matter, moreover it is possible to improve the dissolubility of inorganic phosphate mineral, micro member that can be in activated soil
Element.
Embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to
Example is described in further detail to the present invention, but example is not as a limitation of the invention.
Embodiments of the invention following steps：
It is prepared by S10, first class inoculum；
S101, Lactobacillus plantarum first class inoculum preparation：In the bottled fluid nutrient medium of 500ml drips
400ml, sterile access Lactobacillus plantarum test tube strains, after static gas wave refrigerator 48h it is to plant at 28 DEG C
Thing lactobacillus first class inoculum；
Lactobacillus plantarum first class inoculum culture medium：Sucrose：15g/L, glucose：15g/L, soybean
Molasses：30g/L, peptone：25g/L, K2HPO4：2g/L, NaAc：6g/L, hydrogen citrate
Two ammoniums：2g/L, Tween80：2g/L, MgSO4：0.1g/L, MnSO4：0.1g/L；
S102, saccharomyces cerevisiae first class inoculum preparation：In the bottled fluid nutrient medium of 500ml drips
400ml, sterile access saccharomyces cerevisiae test tube strains, after static gas wave refrigerator 48h it is to make wine at 28 DEG C
Yeast first class inoculum；
Saccharomyces cerevisiae first class inoculum culture medium：Maltose：30g/L, corn flour：20g/L, bean powder：
10g/L, dipotassium hydrogen phosphate：8.0g/L, magnesium sulfate：12.0g/L calcium chloride：6.0g/L；
S103, Trichoderma harzianum first class inoculum preparation：In the bottled fluid nutrient medium of 500ml triangles
150ml, sterile access Trichoderma harzianum test tube strains, is cultivated, condition is 28 DEG C, 120r in shaking table
It is Trichoderma harzianum first class inoculum after/min cultures 48h.
Trichoderma harzianum one-level second class inoculum culture medium：Wheat bran：20g/L, corn flour：15g/L, phosphoric acid
Hydrogen dipotassium：8.0g/L, magnesium sulfate：10.0g/L ammonium nitrate：4.0g/L；
It is prepared by S20, second class inoculum；
S201, Lactobacillus plantarum second class inoculum preparation：In the bottled fluid nutrient medium of 500ml drips
360ml, sterile access Lactobacillus plantarum first class inoculum 40ml, at 28 DEG C after static gas wave refrigerator 48h
As Lactobacillus plantarum second class inoculum；
Lactobacillus plantarum one-level second class inoculum culture medium：Sucrose：15g/L, glucose：15g/L,
Soy molasses：30g/L, peptone：25g/L, K2HPO4：2g/L, NaAc：6g/L, lemon
The sour ammonium of hydrogen two：2g/L, Tween80：2g/L, MgSO4：0.1g/L, MnSO4：0.1g/L.
S202, saccharomyces cerevisiae second class inoculum preparation：In the bottled fluid nutrient medium of 500ml drips
360ml, sterile access saccharomyces cerevisiae first class inoculum 40ml, at 28 DEG C after static gas wave refrigerator 48h i.e.
For saccharomyces cerevisiae second class inoculum；
Saccharomyces cerevisiae one-level second class inoculum culture medium：Maltose：30g/L, corn flour：20g/L,
Bean powder：10g/L dipotassium hydrogen phosphates：8.0g/L, magnesium sulfate：12.0g/L calcium chloride：6.0g/L.
S203, Trichoderma harzianum second class inoculum preparation：In the bottled fluid nutrient medium of 500ml triangles
130ml, sterile access Trichoderma harzianum first class inoculum 20ml, is cultivated, condition 28 in shaking table
DEG C, 120r/min culture 48h after be Trichoderma harzianum second class inoculum；
Trichoderma harzianum one-level second class inoculum culture medium：Wheat bran：20g/L, corn flour：15g/L, phosphoric acid
Hydrogen dipotassium：8.0g/L, magnesium sulfate：10.0g/L ammonium nitrate：4.0g/L.
It is prepared by S30, three-class strain；
S301, Lactobacillus plantarum and saccharomyces cerevisiae complex solid strain preparation：1000 kilograms solid
30 kilograms of Lactobacillus plantarum second class inoculums of body culture medium inoculated and 30 kilograms of saccharomyces cerevisiae two level bacterium
Kind, regulation fermentation material water content is 50% or so, and sealed fermenting is and planted after 15 days at 28 DEG C
Thing lactobacillus and saccharomyces cerevisiae complex solid strain；
Lactobacillus plantarum and the compound three-class strain solid medium of saccharomyces cerevisiae：Wheat bran：60%th, it is beautiful
Ground rice：35%th, glucose：2.0%th, lactose：1.0%th, peptone：1%th, yeast extract：
1.0%.
S302, Lactobacillus plantarum and saccharomyces cerevisiae composite fluid strain preparation：1000L liquid is trained
Support base and be inoculated with 20 kilograms of Lactobacillus plantarum second class inoculums and 20 kilograms of saccharomyces cerevisiae second class inoculums,
Static ferment 15 days of anaerobism is Lactobacillus plantarum and saccharomyces cerevisiae composite fluid strain at 28 DEG C；
Lactobacillus plantarum and the compound three-class strain solid medium of saccharomyces cerevisiae：Sucrose：5g/L, Portugal
Grape sugar：5g/L, soy molasses：30g/L, corn flour：10g/L, K2HPO4：2g/L, citric acid
The ammonium of hydrogen two：1g/L, Tween80：1g/L, MgSO4：0.1g/L, MnSO4：0.1g/L.
S303, Trichoderma harzianum three-class strain preparation：1000 kilograms of solid mediums are inoculated with 50 kilograms
Trichoderma harzianum second class inoculum, regulation fermentation material water content is at 50% or so, 28 DEG C, and tile 5cm shallow-layers
The 7d that ferments is Trichoderma harzianum three-level solid spawn.
Trichoderma harzianum three-class strain culture medium：Wheat bran 80%, corn flour 15%, glucose 2.0%, breast
Sugar 1.0%, peptone 1.0%, yeast extract 1.0%.
S40, biological organic fertilizer fermentation：
Also include the preparation of biological organic fertilizer fermentation process and its fermentation medium：Every 1000 kilograms
Organic fertilizer production fermentation medium adds 50 kilograms of Lactobacillus plantarum three-level composite fluid strains, 50
Kilogram Lactobacillus plantarum three-level complex solid strain, 30 kilograms of Trichoderma harzianum three-class strains, regulation
Fermentation material water content is in 45-55%, raft fermentation, every turning in three days once, after fermentation 15 days,
The fermentate is mixed with diatomite powder by weight 4: 1 and mixed thoroughly, low temperature drying to water content is
20%-30%.
Organic fertilizer fermentation culture medium is：Expanded 65 parts of chicken manure, 43.5 parts of powder of straw, glucose 0.5
Part, 1 part of corn flour.
First class inoculum is individually cultivated, and one-level second class inoculum culture medium is liquid, and HTHP goes out
Used after bacterium.
The foregoing description of the disclosed embodiments, enable professional and technical personnel in the field realize or
Use the present invention.A variety of modifications to these embodiments for those skilled in the art will
Be it will be apparent that generic principles defined herein can not depart from the present invention spirit or
In the case of scope, realize in other embodiments.Therefore, the present invention is not intended to be limited to this
These embodiments shown in text, and be to fit to and principles disclosed herein and features of novelty phase one
The most wide scope caused.
Claims (6)
1. one kind contains diatomaceous microbe soil insecticide preparation method, it is characterised in that bag
Include following steps：
It is prepared by S10, first class inoculum；
It is prepared by S20, second class inoculum；
It is prepared by S30, three-class strain；
S40, biological organic fertilizer fermentation.
2. contain diatomaceous microbe soil insecticide preparation method as claimed in claim 1,
Characterized in that, in the step S10, it is further comprising the steps of：
S101, Lactobacillus plantarum first class inoculum preparation：In the bottled fluid nutrient medium of 500ml drips
400ml, sterile access Lactobacillus plantarum test tube strains, after static gas wave refrigerator 48h it is plant at 28 DEG C
Lactobacillus first class inoculum；
S102, saccharomyces cerevisiae first class inoculum preparation：In the bottled fluid nutrient medium of 500ml drips
400ml, sterile access saccharomyces cerevisiae test tube strains, after static gas wave refrigerator 48h it is wine brewing ferment at 28 DEG C
Female first class inoculum；
S103, Trichoderma harzianum first class inoculum preparation：In the bottled fluid nutrient medium of 500ml triangles
150ml, sterile access Trichoderma harzianum test tube strains, is cultivated, condition is 28 DEG C, 120r in shaking table
It is Trichoderma harzianum first class inoculum after/min cultures 48h.
3. contain diatomaceous microbe soil insecticide preparation method as claimed in claim 2,
Characterized in that, in the step S20, it is further comprising the steps of：
S201, Lactobacillus plantarum second class inoculum preparation：In the bottled fluid nutrient medium of 500ml drips
360ml, sterile access Lactobacillus plantarum first class inoculum 40ml, at 28 DEG C after static gas wave refrigerator 48h i.e.
For Lactobacillus plantarum second class inoculum；
S202, saccharomyces cerevisiae second class inoculum preparation：In the bottled fluid nutrient medium of 500ml drips
360ml, sterile access saccharomyces cerevisiae first class inoculum 40ml, it is after static gas wave refrigerator 48h at 28 DEG C
Saccharomyces cerevisiae second class inoculum；
S203, Trichoderma harzianum second class inoculum preparation：In the bottled fluid nutrient medium of 500ml triangles
130ml, sterile access Trichoderma harzianum first class inoculum 20ml, is cultivated in shaking table, condition be 28 DEG C,
It is Trichoderma harzianum second class inoculum after 120r/min cultures 48h.
4. contain diatomaceous microbe soil insecticide preparation method as claimed in claim 3,
Characterized in that, in the step S30, it is further comprising the steps of：
S301, Lactobacillus plantarum and saccharomyces cerevisiae complex solid strain preparation：1000 kilograms of solids
30 kilograms of Lactobacillus plantarum second class inoculums of culture medium inoculated and 30 kilograms of saccharomyces cerevisiae second class inoculums, adjust
Fermentation material water content is saved 50% or so, sealed fermenting is Lactobacillus plantarum after 15 days at 28 DEG C
With saccharomyces cerevisiae complex solid strain；
S302, Lactobacillus plantarum and saccharomyces cerevisiae composite fluid strain preparation：1000L Liquid Cultures
Base is inoculated with 20 kilograms of Lactobacillus plantarum second class inoculums and 20 kilograms of saccharomyces cerevisiae second class inoculums, 28
Static ferment 15 days of anaerobism is Lactobacillus plantarum and saccharomyces cerevisiae composite fluid strain at DEG C；
S303, Trichoderma harzianum three-class strain preparation：1000 kilograms of solid mediums are inoculated with 50 kilograms
Trichoderma harzianum second class inoculum, regulation fermentation material water content is 50% or so, and tile 5cm at 28 DEG C
Shallow-layer fermentation 7d is Trichoderma harzianum three-level solid spawn.
5. contain diatomaceous microbe soil insecticide preparation method as claimed in claim 4,
Characterized in that, in the step S40, in addition to biological organic fertilizer fermentation process and its fermentation training
Support the preparation of base：Every 1000 kilograms of organic fertilizer production fermentation mediums add 50 kilograms of Lactobacillus plantarums
Three-level composite fluid strain, 50 kilograms of Lactobacillus plantarum three-level complex solid strains, 30 kilograms of Kazakhstan thatches
Trichoderma three-class strain, regulation fermentation material water content is in 45-55%, raft fermentation, every turning one in three days
It is secondary, after fermenting 15 days, the fermentate is mixed with diatomite powder by weight 4: 1 and mixed thoroughly, it is low
It is 20%-30% that temperature, which is dried to water content,.
6. contain diatomaceous microbe soil insecticide preparation method as claimed in claim 1,
Characterized in that, first class inoculum is individually cultivated, one-level second class inoculum culture medium is liquid, high temperature
Used after autoclaving.
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Citations (4)
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|---|---|---|---|---|
| CN1966664A (en) * | 2006-05-12 | 2007-05-23 | 李季 | Composite microorganism bacteria agent for compost fermentation and its producing method and use |
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| CN105198661A (en) * | 2015-08-13 | 2015-12-30 | 李志山 | Biological disinsection organic fertilizer and preparation method thereof |
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- 2016-05-11 CN CN201610303382.4A patent/CN107365215A/en active Pending
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|---|---|---|---|---|
| CN1966664A (en) * | 2006-05-12 | 2007-05-23 | 李季 | Composite microorganism bacteria agent for compost fermentation and its producing method and use |
| CN101422169A (en) * | 2008-12-16 | 2009-05-06 | 广西大学 | Entomogenous fungi powder for controlling pine greedy scale and production method and use thereof |
| CN102888356A (en) * | 2012-09-03 | 2013-01-23 | 新疆农业科学院土壤肥料与农业节水研究所 | Bacillus subtilis, microbial fertilizer prepared by using bacillus subtilis, method for preparing microbial fertilizer by using bacillus subtilis, and application of microbial fertilizer |
| CN105198661A (en) * | 2015-08-13 | 2015-12-30 | 李志山 | Biological disinsection organic fertilizer and preparation method thereof |
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