CN107361723A - Rapid tissue molecular spectrum imaging device - Google Patents
Rapid tissue molecular spectrum imaging device Download PDFInfo
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- CN107361723A CN107361723A CN201710596784.2A CN201710596784A CN107361723A CN 107361723 A CN107361723 A CN 107361723A CN 201710596784 A CN201710596784 A CN 201710596784A CN 107361723 A CN107361723 A CN 107361723A
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- 230000003287 optical effect Effects 0.000 claims abstract description 20
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B1/00—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
- A61B1/00131—Accessories for endoscopes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B1/00—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
- A61B1/00163—Optical arrangements
- A61B1/00165—Optical arrangements with light-conductive means, e.g. fibre optics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B1/00—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor
- A61B1/04—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor combined with photographic or television appliances
- A61B1/043—Instruments for performing medical examinations of the interior of cavities or tubes of the body by visual or photographical inspection, e.g. endoscopes; Illuminating arrangements therefor combined with photographic or television appliances for fluorescence imaging
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
- A61B5/0062—Arrangements for scanning
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
- A61B5/0071—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence by measuring fluorescence emission
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
- A61B5/0075—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence by spectroscopy, i.e. measuring spectra, e.g. Raman spectroscopy, infrared absorption spectroscopy
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
- A61B5/0082—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence adapted for particular medical purposes
- A61B5/0084—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence adapted for particular medical purposes for introduction into the body, e.g. by catheters
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Surgery (AREA)
- Physics & Mathematics (AREA)
- Engineering & Computer Science (AREA)
- Animal Behavior & Ethology (AREA)
- Biomedical Technology (AREA)
- Heart & Thoracic Surgery (AREA)
- Medical Informatics (AREA)
- Molecular Biology (AREA)
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- Pathology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Optics & Photonics (AREA)
- Radiology & Medical Imaging (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention provides a kind of rapid tissue molecular spectrum imaging device, including Optical Transmit Unit, steering unit, scanning element and probe unit, wherein the Optical Transmit Unit is used to launch Line beam；The steering unit is used to turn to the Line beam and through the fluorescence of sample；The direction that the scanning element is used to adjust the Line beam turned to is to progressively scan sample；And the probe unit is used to gather the fluorescence and forms the spatial image and spectral information of the sample.Due to being combined using Line beam with spectrographic detection unit to obtain the spatial image of tissue element and spectral information, the image taking speed of tissue element can not only be greatly improved, real time imagery can be achieved, spectral information assistant analysis hoc scenario (such as tumor analysis) can also be passed through.Because scanning element only carries out one-dimensional scanning, therefore the stability of system can be effectively improved.
Description
Technical field
The present invention relates to medical instruments field, relates more specifically to a kind of rapid tissue molecular spectrum imaging device.
Background technology
Tumour is the major disease of serious threat human health.Numerous studies show that more than 90% tumour derives from epithelium
The lesion of cell, and the variation of molecule and cellular level can occur during cancer occurrence and development.High score based on fibre bundle
Resolution optics based endoscopic imaging technology, the resolution ratio of micron or sub-micron can be reached, make scope multiplication factor up to 1000 times, relatively
In other medical imaging technologies (such as CT, MRI, PET) have it is lossless, real-time, physical examination micrometer little tumour venereal disease become etc. technology
Advantage, it can preferably improve the early diagnostic rate of tumour.The sound end of based endoscopic imaging can be deep into inside live body, complete micron
Level is detected in body real non-destructive, realizes " in body biopsy " without sampling, and new skill is carried out for early stage cellular elements lesion surveying tape
Art means.
The content of the invention
The present invention is proposed in view of above mentioned problem.The invention provides a kind of imaging of rapid tissue molecular spectrum to fill
Put, including Optical Transmit Unit, steering unit, scanning element and spectrographic detection unit, wherein the Optical Transmit Unit is used to launch
Line beam；The steering unit is used to turn to the Line beam and through the fluorescence of sample；The scanning element, which is used to adjust, to be turned
To Line beam direction to progressively scan sample；And the spectrographic detection unit is used to gather described in the fluorescence and formation
The spatial image and spectral information of sample.
Exemplarily, the Optical Transmit Unit includes：Light source, for launching collimated light beam；And line focus device is expanded, if
Put in the exit of the light source, be Line beam for the collimated light beam to be expanded into simultaneously one-dimensional focusing.
Exemplarily, the steering unit is dichroscope.
Exemplarily, the scanning element is single scanning galvanometer or spatial light modulator.
Exemplarily, described device also include be arranged on the scanning element downstream relay unit and it is interior peep unit, its
Described in relay unit be used for by the scanning element scan after Line beam focus on it is described in peep unit；Unit is peeped in described
For the Line beam of focusing to be conducted and focuses on sample and receives the fluorescence that sample sends；The fluorescence is through the relaying
Gathered after unit, the scanning element and the steering unit by the spectrographic detection unit.
Exemplarily, the interior unit of peeping includes coupling object lens and imaging optical fiber bundle, wherein the couplings mirror is arranged on
One end of the imaging light shafts, for the Line beam of the focusing to be coupled into the near-end of the fibre bundle；It is and described
Imaging optical fiber bundle is used to conduct the Line beam entered.
Exemplarily, peeping unit in described also includes miniature object lens, and the miniature object lens are arranged on the imaging light shafts
The other end, the Line beam for the fibre bundle to be conducted focuses on the sample.
Exemplarily, the probe unit includes linear array probe unit, spectrographic detection unit and switch control unit, its
In：The linear array probe unit, for gathering fluorescence and forming the spatial image of sample；The spectrographic detection unit, for adopting
Collection fluorescence simultaneously forms the spectral information of sample；The switch control unit, for the linear array probe unit and the spectrum
Probe unit switches over selection.
Exemplarily, the probe unit also includes the first condenser lens, and first condenser lens is arranged on the line
It is single for the fluorescent foci that the sample is sent to the linear array to be detected between battle array probe unit and the switch control unit
Member.
Exemplarily, the spectrographic detection unit is spectrum camera.
Exemplarily, the probe unit also includes the second condenser lens, and second condenser lens is arranged on the light
Compose between probe unit and the switch control unit, for by the fluorescent foci that the sample is sent to the spectrographic detection list
Member.
Exemplarily, the spectrographic detection unit includes prism-grating-prism, plus lens and the face battle array set gradually
Detector, wherein the prism-grating-prism is used to carry out dispersion light splitting to the fluorescence of steering unit transmission；The remittance
Poly- lens are used for the fluorescent foci after dispersion is divided to the photosurface of the area array cameras；The planar array detector is used for shape
Into the spectral information.
Exemplarily, the probe unit also includes the first condenser lens of the fluorescent foci for the sample to be sent
And/or second condenser lens, wherein：First condenser lens is arranged on the linear array probe unit and the switching control list
Between member；Second condenser lens is arranged between the spectrographic detection unit and the switch control unit.
The probe unit also includes being successively set between the switch control unit and the spectrographic detection unit
Second condenser lens and collimation lens, wherein：Second condenser lens is used for the fluorescent foci for sending the sample；And
The collimation lens is used to collimate the fluorescence after focusing.
Exemplarily, the probe unit also includes slit and/or the optical filtering for being arranged on the second condenser lens downstream
Device, wherein：The slit is used to only allow the fluorescence of focussing plane to pass through；And the filter is used to filter out veiling glare.
The rapid tissue molecular spectrum imaging device is excited using line source to sample, using one-dimensional scanning unit
(such as single scanning galvanometer) is scanned to Line beam, and using spectrographic detection unit to sample excitation optical detection, one-dimensional
Realize that copolymerization is burnt in direction.Due to being combined using Line beam with spectrographic detection unit to obtain the spatial image of tissue element and light
Spectrum information, the image taking speed of tissue element can be not only greatly improved, real time imagery can be achieved, can also be auxiliary by spectral information
Help analysis hoc scenario (such as tumor analysis).Because scanning element only carries out one-dimensional scanning, therefore can effectively improve
The stability of system.
Brief description of the drawings
The embodiment of the present invention is described in more detail in conjunction with the accompanying drawings, above-mentioned and other purpose of the invention,
Feature and advantage will be apparent.Accompanying drawing is used for providing further understanding the embodiment of the present invention, and forms explanation
A part for book, it is used to explain the present invention together with the embodiment of the present invention, is not construed as limiting the invention.In the accompanying drawings,
Identical reference number typically represents same or similar part or step.
Fig. 1 shows the schematic block diagram of rapid tissue molecular spectrum imaging device according to an embodiment of the invention；
Fig. 2 shows the signal of the rapid tissue molecular spectrum imaging device of first group of specific embodiment according to the present invention
Property block diagram；
Fig. 3 shows the light path of the rapid tissue molecular spectrum imaging device of first group of specific embodiment according to the present invention
Schematic diagram；
Fig. 4 shows the signal of the rapid tissue molecular spectrum imaging device of second group of specific embodiment according to the present invention
Property block diagram；And
Fig. 5 shows the light path of the rapid tissue molecular spectrum imaging device of second group of specific embodiment according to the present invention
Schematic diagram.
Embodiment
Become apparent in order that obtaining the object, technical solutions and advantages of the present invention, root is described in detail below with reference to accompanying drawings
According to the example embodiment of the present invention.Obviously, described embodiment is only the part of the embodiment of the present invention, rather than this hair
Bright whole embodiments, it should be appreciated that the present invention is not limited by example embodiment described herein.Described in the present invention
The embodiment of the present invention, those skilled in the art's all other embodiment resulting in the case where not paying creative work
It should all fall under the scope of the present invention.
Fig. 1 schematically shows rapid tissue molecular spectrum imaging device 100 according to an embodiment of the invention
Block diagram.The rapid tissue molecular spectrum imaging device 100 includes Optical Transmit Unit 110, steering unit 120, the and of scanning element 130
Probe unit 160.The rapid tissue molecular spectrum imaging device 100 can be widely applied to each position such as alimentary canal, respiratory tract
Tissue element imaging, realize the early diagnosis of tumour.
Optical Transmit Unit 110 is used to launch Line beam.In one embodiment, as Figure 2-3, Optical Transmit Unit 110
Light source 112 can be included and expand line focus device 114.Light source 112 is used to launch collimated light beam.Light source 112 can be that transmitting is special
The laser of the collimation laser of standing wave length.The particular range of wavelengths can be 20nm-2000nm.Laser in the wave-length coverage
Large-scale fluorophor can be excited.Light source 112 can be quantum-well laser, solid-state laser, gas laser (such as argon
Ion laser) or laser diode.The outlet that line focus device 114 is arranged on light source 112 is expanded, for light source 112 to be sent out
The collimated light beam gone out expands and one-dimensional focusing is Line beam.Extender lens and post lens can be included by expanding line focus device 114.Expand
Beam lens can include two L1, L2, and two extender lenses L1, L2, which coordinate, is expanded the collimated light beam that light source 112 is sent,
To change the diameter of collimated light beam.Post lens include L3, and its light beam one-dimensional focusing after expanding is Line beam and conduction extremely turns
To unit 120.
Steering unit 120 is located at the downstream of Optical Transmit Unit 110, for turning to the Line beam of the transmitting of Optical Transmit Unit 110,
And the Fluoroscopic of sample can be made.In fig. 1-3, solid line is used to represent the Line beam that Optical Transmit Unit 110 is sent, dotted line
For representing that sample is excited the fluorescence sent.Steering unit 120 is used for light and the sample excitation that discrete light-emissive unit 110 is sent
Caused fluorescence.Steering unit 120 can reach more than 90% to the transmissivity of fluorescence, and for other wavelength light substantially
All reflections.Then, the Line beam that Optical Transmit Unit 110 is sent is being reflected to scanning element 130 by steering unit 120.
Along almost all transmits when by steering unit 120 with the fluorescence that Line beam identical light path returns, and conduct single to detecting
Member 160.The steering unit 120 for meeting above-mentioned condition can be dichroscope.Preferably, the wave-length coverage of the dichroscope can be with
In 40nm-2200nm wave-length coverages.
Scanning element 130 is located at the downstream of steering unit 120, one-dimensional sweeping is carried out to the Line beam of steering, for adjusting
The direction of the Line beam of steering is to progressively scan sample.Specifically, Line beam can be the Line beam for example extended in X direction,
The Line beam redirect to the optical component (such as relay unit 140) in downstream by scanning element 130, while carries out Y-direction scanning.
Y-direction is angled with X-direction, such as into 90 degree of right angle.Scanning element 130 is substantially carried out the one-dimensional scanning of Y-direction.This
Sample, the scanning can carried out in an one-dimensional square is coordinated to form entire image with the Line beam of X-direction.As can be seen here, adopt
It can be line by line imaged with Line beam combination probe unit 160, therefore be able to significantly compared to existing point-by-point imaging, image taking speed
Improve.Due to only carrying out the sweeping in one-dimensional square, scanning element 130 can be single scanning galvanometer.The frequency of scanning galvanometer
Rate can be in 10-2000KHz frequency range.Noise can be greatly reduced in the use of single scanning galvanometer, and simplifies dress
The composition and the complexity of control put, improve complete machine stability, while reduce manufacturing cost and maintenance cost.In addition, sweep
It can also be spatial light modulator to retouch unit 130.Spatial light modulator compared to scanning galvanometer for, cost is of a relatively high.
The rapid tissue molecular spectrum imaging device 100 also includes the relay unit 140 for being arranged on the downstream of scanning element 130
Unit 150 is peeped with interior.Fig. 2-3 shows the rapid tissue molecular spectrum imaging device according to a specific embodiment of the invention
200 index path and block diagram.In Fig. 2-3 identical reference is employed with the same or analogous parts of Fig. 1.Below will ginseng
Relay unit 140 in being described in detail according to a particular embodiment of the invention according to Fig. 2-3, interior peep unit 150 and detection is single
The specific implementation of member 160.
Relay unit 140 be used for scanning element 130 is scanned after Line beam focus in peep unit 150.Relay unit
140 usually lens groups, such as lens L4, L5.
Inside peep unit 150 to be used to conduct the Line beam that relay unit 140 focuses on and focus on sample, and receive sample
The fluorescence that product are sent.Gathered after the repeated unit 140 of the fluorescence and steering unit 120 by probe unit 160.Inside peep unit 150
Coupling object lens 152, miniature object lens 156 and the imaging being coupling between coupling object lens 152 and miniature object lens 156 can be included
Fibre bundle 154.Relay unit 140 can include two relay lens L4, L5, and they cooperate in the Line beam after scanning
After the rear pupil to the interior coupling object lens 152 peeped in unit 150.Coupling object lens 152 enter for Line beam to be coupled to (such as focusing)
Enter the near-end of imaging optical fiber bundle 154 (close to one end of operating personnel).Imaging optical fiber bundle 154 is used to conduct Line beam to imaging
The distal end (one end away from operating personnel) of fibre bundle 154.Miniature object lens 156 are used for the laser for conducting imaging optical fiber bundle 154
Focus in the detection faces of sample.Detection faces can be located at the required depth below sample surfaces.At the detection faces of sample
Fluorogen be excited to send fluorescence.Fluorescence signal is collected by miniature object lens 156, imaged fibre bundle 154, coupling object lens 152
Conducted with relay unit 140, scanning element 130 reflects, and enters probe unit 160 through steering unit 120.Imaging optical fiber bundle
The quantity of light shafts included by 154 can be more than ten.Miniature object lens 156 are not essential.Less demanding to definition
In the case of, it is alternatively possible to omit miniature object lens 156.Miniature object lens 156 can be designed to that alimentary canal, breathing can be extend into
In road etc., it is in contact with the surface of alimentary canal, respiratory tract etc..
Probe unit 160 gathers peeps unit 150, relay unit 140, scanning element 130 and steering unit 120 in warp successively
The fluorescence of return, and form the spatial image and spectral information of sample.The spatial image of the sample includes the detection faces of sample
Two dimensional image.The spectral information including sample be excited caused by fluorescence in the Energy distribution of different-waveband, be used to help obtain
Take organizational information (such as analyzing tumour).In a specific embodiment, it is single can to include linear array detection for probe unit 160
Member 162, spectrographic detection unit 164 and switch control unit 166, as Figure 2-3.
Linear array probe unit 162 is used to gather fluorescence and forms the spatial image of sample.Linear array probe unit 162 can be
Various types of line-scan digital cameras, such as CCD (charge coupled cell) line-scan digital cameras or CMOS (complementary metal oxide semiconductor)
Line-scan digital camera etc..The image taking speed of linear array probe unit 162 is in the range of tens frames to several ten million frames.Preferably, the detection
Unit 160 is also arranged on linear array probe unit 162 and switch control unit including the first condenser lens L6, the first condenser lens L6
Between 166, as shown in figure 3, for by the fluorescent foci that sample is sent to linear array probe unit 162, with into sharply defined image.
Spectrographic detection unit 164 is used to gather fluorescence and form the spectral information of sample, hereinafter will be to spectrographic detection unit
164 are described in detail.
Switch control unit 166 is used to switch over selection to linear array probe unit 162 and spectrographic detection unit 164, with
Optionally obtain spatial image or spectral information.Switch control unit 166 for example carries out selectivity to the transmission path of fluorescence
Ground switches, and fluorescence is entered linear array probe unit 162 or spectrographic detection unit 164.Exemplarily, switch control unit 166 can
Think reflective mirror, digital micro-mirror device (DMD) or spatial light modulator.Wherein, digital micro-mirror device can by controlling break-make,
Realize the projection or reflection of light path.Schematically shown in Fig. 3 using digital micro-mirror device and be used as switch control unit 166
Embodiment.In use, controlling the conducting of switch control unit 166 to make Fluoroscopic first, space is carried out by linear array probe unit 162
Imaging, searches out target area (such as tumour)；Then when it is expected to make a concrete analysis of the target area, control switching control
Unit 166 processed is blocked, and by fluorescent reflection to spectrographic detection unit 164, the light of the target area is obtained by spectrographic detection unit 164
Spectrum information.In the case of switch control unit 166 is using reflective mirror or spatial light modulator, those skilled in the art can be with
Modification is carried out to light path according to principle disclosed in the present application.
In a preferred embodiment (such as embodiment shown in Fig. 2-3), spectrographic detection unit 164 is spectrum camera.
Spectrum camera can be the existing or following various types of spectrum cameras being likely to occur, for example, U.S. Rui Sangnan
(RESONON) the Pika L-types spectrum camera of company and this one hundred plucked instrument nurse of Finland
(Specim) FX10 type spectrum cameras of company etc., as long as the spectrum of sample can be formed according to the fluorescence collected
Information.Preferably, the probe unit 160 is also arranged on spectrum spy including the second condenser lens L7, the second condenser lens L7
Survey between unit 164 and switch control unit 166, as shown in figure 3, for by the fluorescent foci that sample is sent to spectrographic detection list
Member 164, to obtain relatively reliable spectral information.
In a further advantageous embodiment, shown as shown in Fig. 4-5 of another group of specific embodiment, spectrographic detection unit
164 can include prism-grating-prism (PGP prisms) 164a, plus lens 164b and the planar array detector that set gradually
164c.After spectrum function is switched to by switch control unit 166, PGP prisms 164a is used for what steering unit 120 was transmitted
Fluorescence carries out dispersion light splitting.Plus lens 164b is used for the fluorescent foci after dispersion is divided to the photosurface of area array cameras 166
On.The port number of spectrum of the plus lens 164b quantity with being obtained is relevant, that is, it is expected to obtain the spectrum picture of more multichannel,
Then use more plus lens.Planar array detector 164c is used for the spectral information for forming sample.Planar array detector 166 can be
Various types of area array cameras, such as CCD (charge coupled cell) area array cameras or CMOS (complementary metal oxide semiconductor)
Area array cameras etc..
It is further preferred that as shown in figure 5, in light path, probe unit 160 preferably also includes the second condenser lens
L7 and collimation lens L8, they set successively between switch control unit 166 and spectrographic detection unit 164, along optical path direction
Put, as illustrated in figures 4-5.Second condenser lens L7 is used for the fluorescent foci for sending sample.Line beam after focusing illuminates sample
The fluorescence sent is received, and by the steering and scanning of scanning element 130, the fluorescence that all rows of sample are sent finally all is visited
Survey unit 160 receive, and according to scanning trajectory alignment into spectral cube data, and then can quickly obtain tissue spectrum letter
Breath.Collimation lens L8 is used to collimate the fluorescence after focusing.Alternatively, the second condenser lens L7 and collimation lens L8 it
Between can be provided with slit (not shown), slit is used to only allow the fluorescence of focussing plane to pass through.The size of slit can be several
In the range of ten nanometers to tens millimeters.The presence of slit causes the veiling glare outside focussing plane to be blocked.Alternatively, detect
Unit 160 can also include filter.Filter (not shown) is arranged on the second condenser lens L7 downstreams, i.e., is focused on second saturating
Between mirror L7 and collimation lens L8, for filtering out veiling glare.In having the embodiment of slit, it is poly- that filter can be arranged on second
Between focus lens L7 and slit.
Put it briefly, the collimated light beam that light source 112 is sent, expand through expanding line focus device 114 and one-dimensional pool linear light
Beam, steering unit 120 turn back Line beam, scanning element 130 by Line beam by relay unit 140 be coupled into peep unit
150 and one-dimensional scanning is carried out, inside peep unit 150 and conduct laser beam to sample, inspire fluorescence and be passed back to probe unit 160
Form spatial image and spectral information.
Exemplarily, the data that probe unit collects can be sent to computer, are received by computer and are handled.This
Outside, the computer can also be launched scanning element (such as frequency of galvanometer etc.), the exposure of probe unit and gain and light
Transmission power of unit etc. is controlled.
The rapid tissue molecular spectrum imaging device 100 is excited using line source to sample, using one-dimensional scanning list
First 130 (such as single scanning galvanometers) are scanned to Line beam, and using probe unit 160 to sample excitation optical detection,
One-dimensional square realizes that copolymerization is burnt.Due to being combined using Line beam with probe unit 160 to obtain the spatial image of tissue element
And spectral information, the image taking speed of tissue element can be not only greatly improved, real time imagery can be achieved, can also be believed by spectrum
Cease assistant analysis hoc scenario (such as tumor analysis).Because scanning element 130 only carries out one-dimensional scanning, therefore can have
Imitate the stability of raising system.
Although describe example embodiment by reference to accompanying drawing here, it should be understood that above-mentioned example embodiment is merely exemplary
, and be not intended to limit the scope of the invention to this.Those of ordinary skill in the art can carry out various changes wherein
And modification, it is made without departing from the scope of the present invention and spiritual.All such changes and modifications are intended to be included in appended claims
Within required the scope of the present invention.
In several embodiments provided herein, it should be understood that disclosed apparatus and method, it can be passed through
Its mode is realized.For example, apparatus embodiments described above are only schematical, for example, the division of the unit, only
Only a kind of division of logic function, there can be other dividing mode when actually realizing, such as multiple units or component can be tied
Another equipment is closed or is desirably integrated into, or some features can be ignored, or do not perform.
In the specification that this place provides, numerous specific details are set forth.It is to be appreciated, however, that the implementation of the present invention
Example can be put into practice in the case of these no details.In some instances, known method, structure is not been shown in detail
And technology, so as not to obscure the understanding of this description.
Similarly, it will be appreciated that in order to simplify the present invention and help to understand one or more of each inventive aspect,
To the present invention exemplary embodiment description in, each feature of the invention be grouped together into sometimes single embodiment, figure,
Or in descriptions thereof.However, the method for the invention should be construed to reflect following intention：It is i.e. claimed
Application claims features more more than the feature being expressly recited in each claim.More precisely, such as corresponding power
As sharp claim reflects, its inventive point is the spy that can use all features less than some disclosed single embodiment
Levy to solve corresponding technical problem.Therefore, it then follows thus claims of embodiment are expressly incorporated in this specific
Embodiment, wherein each claim is in itself as separate embodiments of the invention.
It will be understood to those skilled in the art that in addition to mutually exclusive between feature, any combinations pair can be used
All features and so disclosed any method disclosed in this specification (including adjoint claim, summary and accompanying drawing)
Or all processes or unit of equipment are combined.Unless expressly stated otherwise, this specification (including adjoint right will
Ask, make a summary and accompanying drawing) disclosed in each feature can be replaced by the alternative features for providing identical, equivalent or similar purpose.
In addition, it will be appreciated by those of skill in the art that although some embodiments described herein include other embodiments
In included some features rather than further feature, but the combination of the feature of different embodiments means in of the invention
Within the scope of and form different embodiments.For example, in detail in the claims, embodiment claimed it is one of any
Mode it can use in any combination.
It should be noted that the present invention will be described rather than limits the invention for above-described embodiment, and ability
Field technique personnel can design alternative embodiment without departing from the scope of the appended claims.In the claims,
Any reference symbol between bracket should not be configured to limitations on claims.Word "comprising" does not exclude the presence of not
Element or step listed in the claims.Word "a" or "an" before element does not exclude the presence of multiple such
Element.The present invention can be by means of including the hardware of some different elements and being come by means of properly programmed computer real
It is existing.In if the unit claim of equipment for drying is listed, several in these devices can be by same hardware branch
To embody.The use of word first, second, and third does not indicate that any order.These words can be explained and run after fame
Claim.
The foregoing is only a specific embodiment of the invention or the explanation to embodiment, protection of the invention
Scope is not limited thereto, any one skilled in the art the invention discloses technical scope in, can be easily
Expect change or replacement, should all be included within the scope of the present invention.Protection scope of the present invention should be with claim
Protection domain is defined.
Claims (14)
1. a kind of rapid tissue molecular spectrum imaging device, including Optical Transmit Unit, steering unit, scanning element and detection are single
Member, wherein：
The Optical Transmit Unit is used to launch Line beam；
The steering unit is used to turn to the Line beam and through the fluorescence of sample；
The direction that the scanning element is used to adjust the Line beam turned to is to progressively scan sample；And
The probe unit is used to gather the fluorescence and forms the spatial image and spectral information of the sample.
2. device as claimed in claim 1, wherein, the Optical Transmit Unit includes：
Light source, for launching collimated light beam；And
Line focus device is expanded, is arranged on the exit of the light source, is line for the collimated light beam to be expanded into simultaneously one-dimensional focusing
Light beam.
3. device as claimed in claim 1, wherein, the steering unit is dichroscope.
4. device as claimed in claim 1, wherein, the scanning element is single scanning galvanometer or spatial light modulator.
5. device as claimed in claim 1, wherein, described device also includes the relaying list for being arranged on the scanning element downstream
It is first and it is interior peep unit, wherein：
The relay unit, which is used to focus on the Line beam after scanning element scanning in described, peeps unit；
Unit is peeped in described to be used to conduct the Line beam of focusing and focus on sample and receive the fluorescence that sample is sent；
The fluorescence is adopted after the relay unit, the scanning element and the steering unit by the spectrographic detection unit
Collection.
6. device as claimed in claim 5, wherein, the interior unit of peeping includes coupling object lens and imaging optical fiber bundle, wherein
The coupling object lens are arranged on one end of the imaging light shafts, described for the Line beam of the focusing to be coupled into
The near-end of fibre bundle；And
The imaging optical fiber bundle is used to conduct the Line beam entered.
7. device as claimed in claim 6, wherein, the interior unit of peeping also includes miniature object lens, and the miniature object lens are set
In the other end of the imaging light shafts, the Line beam for the fibre bundle to be conducted focuses on the sample.
8. device as claimed in claim 1, wherein, the probe unit include linear array probe unit, spectrographic detection unit and
Switch control unit, wherein：
The linear array probe unit, for gathering fluorescence and forming the spatial image of sample；
The spectrographic detection unit, for gathering fluorescence and forming the spectral information of sample；
The switch control unit, for switching over selection to the linear array probe unit and the spectrographic detection unit.
9. device as claimed in claim 8, wherein, the probe unit also includes the first condenser lens, and described first focuses on
Lens are arranged between the linear array probe unit and the switch control unit, for the fluorescent foci for sending the sample
To the linear array probe unit.
10. device as claimed in claim 8, wherein, the spectrographic detection unit is spectrum camera.
11. device as claimed in claim 10, wherein, the probe unit also includes the second condenser lens, and described second is poly-
Focus lens are arranged between the spectrographic detection unit and the switch control unit, and the fluorescence for the sample to be sent gathers
Jiao arrives the spectrographic detection unit.
12. device as claimed in claim 8, wherein, the spectrographic detection unit includes the prism-grating-rib set gradually
Mirror, plus lens and planar array detector, wherein
The prism-grating-prism is used to carry out dispersion light splitting to the fluorescence of steering unit transmission；
The plus lens is used for the fluorescent foci after dispersion is divided to the photosurface of the area array cameras；
The planar array detector is used to form the spectral information.
13. device as claimed in claim 12, wherein, the probe unit also includes being successively set on the switching control list
First the second condenser lens and collimation lens between the spectrographic detection unit, wherein：
Second condenser lens is used for the fluorescent foci for sending the sample；And
The collimation lens is used to collimate the fluorescence after focusing.
14. the device as described in claim 11 or 13, wherein, the probe unit also includes being arranged on second focusing thoroughly
The slit and/or filter in mirror downstream, wherein：
The slit is used to only allow the fluorescence of focussing plane to pass through；And
The filter is used to filter out veiling glare.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710596784.2A CN107361723B (en) | 2017-07-20 | 2017-07-20 | Quick tissue molecular spectrum imaging device |
| PCT/CN2018/091980 WO2019015439A1 (en) | 2017-07-20 | 2018-06-20 | Device for fast spectral imaging of tissue molecule |
| TW107124994A TWI692342B (en) | 2017-07-20 | 2018-07-19 | Rapid tissue molecule spectral imaging device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710596784.2A CN107361723B (en) | 2017-07-20 | 2017-07-20 | Quick tissue molecular spectrum imaging device |
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| Publication Number | Publication Date |
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| CN107361723A true CN107361723A (en) | 2017-11-21 |
| CN107361723B CN107361723B (en) | 2024-02-13 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201710596784.2A Active CN107361723B (en) | 2017-07-20 | 2017-07-20 | Quick tissue molecular spectrum imaging device |
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| Country | Link |
|---|---|
| CN (1) | CN107361723B (en) |
| TW (1) | TWI692342B (en) |
| WO (1) | WO2019015439A1 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019015439A1 (en) * | 2017-07-20 | 2019-01-24 | 无锡海斯凯尔医学技术有限公司 | Device for fast spectral imaging of tissue molecule |
| WO2019015438A1 (en) * | 2017-07-20 | 2019-01-24 | 无锡海斯凯尔医学技术有限公司 | Device for fast imaging of tissue molecule |
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Also Published As
| Publication number | Publication date |
|---|---|
| WO2019015439A1 (en) | 2019-01-24 |
| TW201907858A (en) | 2019-03-01 |
| CN107361723B (en) | 2024-02-13 |
| TWI692342B (en) | 2020-05-01 |
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