CN208837875U - Tomography endoscopic microscopic imaging device - Google Patents
Tomography endoscopic microscopic imaging device Download PDFInfo
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- CN208837875U CN208837875U CN201720889338.6U CN201720889338U CN208837875U CN 208837875 U CN208837875 U CN 208837875U CN 201720889338 U CN201720889338 U CN 201720889338U CN 208837875 U CN208837875 U CN 208837875U
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Abstract
The embodiments of the present invention provide a kind of tomography endoscopic microscopic imaging device, including light emitting unit, structure light unit, steering unit and face battle array probe unit, wherein the light emitting unit is for emitting light beam；The structure light unit is used to the light beam being changed into structure light；The steering unit is used to turn to the structure light and the fluorescence through sample；And the face battle array probe unit is for acquiring the fluorescence.The tomography endoscopic microscopic imaging device excites sample using area source, and using face battle array probe unit can greatly improve the image taking speed of tissue element, it can be achieved that real time imagery to sample excitation optical detection.In addition, in tomography endoscopic microscopic imaging device use structure light unit, solve wide field imaging itself as focussing plane upper and lower level bias light interference and caused by problem of image blurring.
Description
Technical field
The utility model relates to medical instruments fields, relate more specifically to a kind of tomography endoscopic microscopic imaging device.
Background technique
Tumour is to seriously threaten the major disease of human health.Nearly 30 years, global cancer (malignant tumour) morbidity number with
Average annual 3%~5% speed increase, cancer have become one of most important cause of the death of the mankind.The discovery of clinical research at present, tumour are early
Phase is not easy excision with transfer, therefore, the early detection of tumour, early diagnosis be improve oncotherapy is horizontal, reduce treatment at
Originally, the key of quality of life after improving more.A large number of studies show that lesion of 90% or more the tumour from epithelial cell, and
The variation of molecule and cellular level can occur during cancer occurrence and development.High-resolution optical based endoscopic imaging based on fiber optic bundle
Technology can reach the resolution ratio of micron or sub-micron, make scope amplification factor up to 1000 times, relative to other medical imaging skills
Art (such as CT, MRI, PET) have it is lossless, real-time, physical examination micrometer little tumour venereal disease become etc. technical advantages, can be preferably
Improve the early diagnostic rate of tumour.The sound end of based endoscopic imaging can be deep into inside living body, complete micron order in body real non-destructive
" in body biopsy " without sampling is realized in detection, and new technological means is carried out for early stage cellular elements lesion surveying tape.
Utility model content
The utility model is proposed in view of the above problem.The utility model provides a kind of tomography endoscopic micro-imaging
Device, including light emitting unit, structure light unit, steering unit and face battle array probe unit, wherein the light emitting unit is used for
Emit light beam；The structure light unit is used to the light beam being changed into structure light；The steering unit is for turning to the knot
Structure light and the fluorescence for penetrating sample；And the face battle array probe unit is for acquiring the fluorescence.
Illustratively, the light emitting unit includes: light source, for emitting collimated light beam；And component is expanded, setting exists
The exit of the light source, for expanding the collimated light beam.
Illustratively, the component that expands includes the narrowband light filter and beam expander set gradually, wherein the narrowband is filtered
Light device is for filtering to the collimated light beam；The beam expander is for expanding the light beam after optical filtering.
Illustratively, the steering unit is dichroscope.
Illustratively, the structure light unit includes: digital micro-mirror device；Or spatial light modulator；Or grating and
Control the mobile driver of the grating.
Illustratively, described device further includes being arranged in the steering unit downstream to peep unit, it is described in peep unit
For by the beam conduction of steering and focus on sample and receive sample sending fluorescence；The fluorescence is single through the steering
It is acquired after member by the face battle array probe unit.
Illustratively, the interior unit of peeping includes coupling object lens and imaging optical fiber bundle, wherein couplings mirror setting exists
One end of the imaging optical fiber bundle, for the light beam coupling of the focusing to be entered to the proximal end of the fiber optic bundle；And it is described at
As fiber optic bundle is used to conduct the light beam entered.
Illustratively, peeping unit in described further includes miniature object lens, and the miniature object lens are arranged in the imaging optical fiber bundle
The other end, the light beam for conducting the fiber optic bundle focuses on the sample.
Illustratively, the face battle array probe unit includes the condenser lens and planar array detector set gradually, wherein described
Condenser lens is used for the fluorescent foci for issuing the sample；The planar array detector is for acquiring fluorescence signal.
Illustratively, the face battle array probe unit further includes long logical optical filter, and the length leads to optical filter setting described poly-
Between focus lens and the planar array detector, for filtering out stray light.
The tomography endoscopic microscopic imaging device excites sample using area source, and using face battle array probe unit is to sample
Product excite optical detection, can greatly improve the image taking speed of tissue element, it can be achieved that real time imagery.In addition, aobvious in tomography endoscopic
In micro- imaging device use structure light unit, solve wide field imaging itself due to focussing plane upper and lower level bias light interference and
Caused by problem of image blurring.
Detailed description of the invention
The utility model embodiment is described in more detail in conjunction with the accompanying drawings, the utility model above-mentioned and its
Its purpose, feature and advantage will be apparent.Attached drawing is used to provide to further understand the utility model embodiment, and
And part of specification is constituted, it is used to explain the utility model together with the utility model embodiment, not constitute to this reality
With novel limitation.In the accompanying drawings, identical reference label typically represents same or similar component or step.
Fig. 1 shows the schematic block diagram of the tomography endoscopic microscopic imaging device according to the utility model one embodiment；
And
Fig. 2 shows the light path schematic diagrams according to the tomography endoscopic microscopic imaging device of the utility model one embodiment.
Specific embodiment
In order to enable the purpose of this utility model, technical solution and advantage become apparent, retouch in detail below with reference to accompanying drawings
State example embodiment according to the present utility model.Obviously, described embodiment is only that a part of the utility model is implemented
Example, rather than the whole embodiments of the utility model, it should be appreciated that the utility model is not by the limit of example embodiment described herein
System.Based on the utility model embodiment described in the utility model, those skilled in the art are not being made the creative labor
In the case where obtained all other embodiment should all fall within the protection scope of the utility model.
Fig. 1 and Fig. 2 schematically shows the tomography endoscopic micro-imaging according to the utility model one embodiment
The block diagram and index path of device 100.The tomography endoscopic microscopic imaging device 100 includes light emitting unit 110, structure light unit
150, steering unit 120 and face battle array probe unit 140.The tomography endoscopic microscopic imaging device 100 can be widely applied to alimentary canal,
The tissue element at each position such as respiratory tract is imaged, and realizes the early diagnosis of tumour.
Light emitting unit 110 is for emitting light beam.In one embodiment, light emitting unit 110 may include light source 112
With expand component 114.Light source 112 is for emitting collimated light beam.Light source 112 can swashing for the collimation laser of transmitting specific wavelength
Light device.The particular range of wavelengths can be 20nm-2000nm.Laser in the wave-length coverage can excite large-scale fluorescence
Body.Light source 112 can be quantum-well laser, solid-state laser, gas laser (such as argon ion laser) or laser
Diode.The outlet that light source 112 is arranged in component 114 is expanded, the collimated light beam for issuing light source 112 expands.At one
In preferred embodiment, expanding component 114 may include the narrowband light filter (not shown) and beam expander set gradually.Narrow-band-filter
The collimated light beam that device is used to issue light source 112 filters.Narrowband light filter can filter out the light of required wavelength, such as permit
Perhaps the light of 500nm-600nm penetrates narrowband light filter, for exciting large-scale fluorescence.Beam expander may include two and expand
Lens L1, L2, they, which cooperate, to expand by the light beam of narrowband light filter, to change the diameter of collimated light beam.
Structure light unit 150 is used to the light beam that light emitting unit 110 emits being changed into structure light, hereinafter will be to structure light
Multiple embodiments of unit 150 are described in detail.
Steering unit 120 is located at the downstream of structure light unit 150, for steering structure light unit 150 formed structure light,
And the Fluoroscopic of sample can be made.In fig 1 and 2, solid line is used to indicate the light beam that light emitting unit 110 issues, dotted line
For indicating that sample is excited the fluorescence issued.Structure light and sample of the steering unit 120 for the generation of separated structure light unit 150
Excite the fluorescence generated.Steering unit 120 can achieve 90% or more to the transmissivity of fluorescence, and for the light base of other wavelength
All reflections in sheet.Then, the structure light that structure light unit 150 generates peeps unit in being reflected to when passing through steering unit 120
130.The fluorescence returned along optical path identical with light beam almost all when by steering unit 120 transmits, and conducts to face battle array
Probe unit 140.The steering unit 120 for meeting above-mentioned condition can be dichroscope.Preferably, the wavelength model of the dichroscope
Enclosing can be in 40nm-2200nm wave-length coverage.
The tomography endoscopic microscopic imaging device 100 further includes being arranged in 120 downstream of steering unit to peep unit 130.It is interior
Unit 130 is peeped for by beam conduction that steering unit 120 turns to and focusing on sample, and receives the glimmering of sample sending
Light.It is acquired after the diverted unit 120 of the fluorescence by face battle array probe unit 140.In a preferred embodiment, as shown in Fig. 2, it is interior
Peep unit 130 may include coupling object lens 132, miniature object lens 136 and be coupled in coupling object lens 132 and miniature object lens 136 it
Between imaging optical fiber bundle 134.Couple the proximal end that object lens 132 are used to enter light beam coupling (such as focusing) imaging optical fiber bundle 134
(close to one end of operator).Imaging optical fiber bundle 134 is used for the distal end by beam conduction to imaging optical fiber bundle 134 (far from behaviour
Make one end of personnel).Miniature object lens 136 are used to focus on the laser that imaging optical fiber bundle 134 conducts the detection faces of sample.Inspection
Survey face can be located at sample surfaces required depth below.Fluorogen at the detection faces of sample is excited to issue fluorescence.It is glimmering
Optical signal is collected by miniature object lens 136, and imaged fiber optic bundle 134 and coupling object lens 132 conduct, pass through steering unit 120 into
Enter face battle array probe unit 140.The quantity of light shafts included by imaging optical fiber bundle 134 can be greater than ten.Miniature object lens 136
It is not required.
In detection optical path, face battle array probe unit 140 acquisition successively peeps what unit 130 and steering unit 120 returned in warp
Fluorescence.In a preferred embodiment, battle array probe unit 140 in face includes condenser lens 142 and planar array detector 146.It focuses saturating
Mirror 142 is used for the fluorescent foci for issuing sample.Fluorescence after focusing is photosensitive on the photosurface of planar array detector 146.Face battle array
Detector 146 can be various types of area array cameras, such as CCD (charge coupled cell) area array cameras or CMOS (complementary gold
Belong to oxide semiconductor) area array cameras etc..The image taking speed of planar array detector 146 is in the range of tens frames to several ten million frames.
Face battle array probe unit 140 is capable of forming a width complete image every time, i.e. the image taking speed of the tomography endoscopic microscopic imaging device is
The image taking speed of face battle array probe unit 140, and then observable tissue element image can be fast implemented.Optionally, face battle array detection
Unit 140 further includes long logical optical filter.Long logical optical filter (not shown) setting condenser lens 142 and planar array detector 146 it
Between, for filtering out stray light.
In summary, the collimated light beam that light source 112 issues, is expanded component 114 and is expanded, changed by structure light unit 150
For structure light, which is turned back and peeps unit 130 in entrance by steering unit 120, inside peeps unit 130 for beam conduction to sample
Product inspire fluorescence and are passed back to face battle array probe unit 140 being imaged.
Illustratively, the collected data of planar array detector can be sent to computer, be received and processed by computer.This
Outside, the computer can also exposure to structure light unit, planar array detector and gain and light emitting unit transmission power
Etc. being controlled.
Structure light unit 150 is arranged between light emitting unit 110 and steering unit 120, is used for light emitting unit 110
The light beam of sending is changed into structure light.The structure light belongs to the light beam after characterizing.Structure light unit 150 passes through modulation light
Phase achievees the purpose that Light Modulation.
In a preferred embodiment, structure light unit 150 may include grating and the driver for controlling grating movement
(such as motor).The grating can be cosine grating.The light beam that light emitting unit 110 issues is by optical grating projection to sample
On, form Structured Illumination.When each period (i.e. grating moving period) forms three width source images, each moving grating period
1/3, be equivalent to 2 π/3 of grating pattern phase shift.The film speed of face battle array probe unit 140 is mobile synchronous with grating simultaneously.Three
Secondary phase shift (0,2 π/3,4 π/3) obtains three width source images of sample, and a width tomographic map of sample is obtained using structured light reconstruction
Picture.For example, if it is desired to which the frame per second reached is 10 frames/second, then grating needs movement 30 times per second, to form 30 width source figures
Picture.In addition, grating can also move 6 times or 9 inferior, accordingly, the 1/6 or 1/ of each phase-shifted grating period in one cycle
9 etc..The algorithm of " structured light reconstruction " can be it is existing or the future may appear various algorithms, due to structured light reconstruction
It is not belonging to the utility model point of the utility model, therefore the utility model does not describe it in further detail.The grating can
Think every millimeter of grating for being greater than or equal to 10 lines pair, for example, 10 lines it is right/millimeter, 20 lines are right/millimeter, 30 lines are right/millimeter or 40
Line is right/millimeter etc..
In a further advantageous embodiment, which can also use spatial light modulator.Space light modulation
Device can carry out Light Modulation in the phase of active control modulated light.It loads the information into one-dimensional or two-dimentional in which can be convenient
Light field in, using the wide bandwidth of light, multi-channel parallel processing the advantages that the information of load is quickly handled.
In a further preferred embodiment, which can also use existing various digital micro-mirror devices
(DMD).DMD be it is a kind of be electronically entered, optics output MEMS (MEMS), internal array element is turned over by being electronically entered
Row control is rotated into, to form desired grating, thus operator can execute high speed, efficiently and reliable empty by the system
Between light modulation.
Structured light reconstruction technology is used in tomography endoscopic microscopic imaging device, solves wide field imaging itself due to focusing
The interference of plane upper and lower level bias light and caused by problem of image blurring.
Although describing example embodiment by reference to attached drawing here, it should be understood that above example embodiment are only exemplary
, and be not intended to the scope of the utility model limited to this.Those of ordinary skill in the art can carry out various wherein
Change and modify, without departing from the scope of the utility model and spirit.All such changes and modifications are intended to be included in appended
Within the scope of the utility model required by claim.
In several embodiments provided herein, it should be understood that disclosed device and method can pass through it
Its mode is realized.For example, apparatus embodiments described above are merely indicative, for example, the division of the unit, only
Only a kind of logical function partition, there may be another division manner in actual implementation, such as multiple units or components can be tied
Another equipment is closed or is desirably integrated into, or some features can be ignored or not executed.
In the instructions provided here, numerous specific details are set forth.It is to be appreciated, however, that the utility model
Embodiment can be practiced without these specific details.In some instances, be not been shown in detail well known method,
Structure and technology, so as not to obscure the understanding of this specification.
Similarly, it should be understood that in order to simplify the utility model and help to understand one in each utility model aspect
Or it is multiple, in the description to the exemplary embodiment of the utility model, each feature of the utility model is divided together sometimes
Group is into single embodiment, figure or descriptions thereof.However, the method for the utility model should not be construed to reflect
Following to be intended to: the requires of the utility model i.e. claimed is more more than feature expressly recited in each claim
Feature.More precisely, practical novel point is can be with less than some as corresponding claims reflect
The features of all features of disclosed single embodiment solves corresponding technical problem.Therefore, it then follows specific embodiment
Thus claims are expressly incorporated in the specific embodiment, wherein each claim itself is as the utility model
Separate embodiments.
It will be understood to those skilled in the art that any combination pair can be used other than mutually exclusive between feature
All features disclosed in this specification (including adjoint claim, abstract and attached drawing) and so disclosed any method
Or all process or units of equipment are combined.Unless expressly stated otherwise, this specification (is wanted including adjoint right
Ask, make a summary and attached drawing) disclosed in each feature can be replaced with an alternative feature that provides the same, equivalent, or similar purpose.
In addition, it will be appreciated by those of skill in the art that although some embodiments described herein include other embodiments
In included certain features rather than other feature, but the combination of the feature of different embodiments mean it is practical new in this
Within the scope of type and form different embodiments.For example, in detail in the claims, embodiment claimed it is any
One of can in any combination mode come using.
The utility model is limited it should be noted that above-described embodiment illustrates rather than the utility model,
And those skilled in the art can be designed alternative embodiment without departing from the scope of the appended claims.In right
In it is required that, any reference symbol between parentheses should not be configured to limitations on claims.Word "comprising" is not arranged
Except there are element or steps not listed in the claims.Word "a" or "an" located in front of the element does not exclude the presence of more
A such element.The utility model can be by means of including the hardware of several different elements and by means of properly programmed
Computer is realized.In the unit claims listing several devices, several in these devices can be by same
One hardware branch embodies.The use of word first, second, and third does not indicate any sequence.It can be by these lists
Word is construed to title.
Above description is only a specific implementation of the present invention or to the explanation of specific embodiment, this is practical new
The protection scope of type is not limited thereto, the technology model that anyone skilled in the art discloses in the utility model
In enclosing, it can easily think of the change or the replacement, should be covered within the scope of the utility model.The protection of the utility model
Range should be subject to the protection scope in claims.
Claims (10)
1. a kind of tomography endoscopic microscopic imaging device, including light emitting unit, structure light unit, steering unit and face battle array detection are single
Member, wherein
The light emitting unit is for emitting light beam；
The structure light unit is used to the light beam being changed into structure light；
The steering unit is used to turn to the structure light and the fluorescence through sample；And
The face battle array probe unit is for acquiring the fluorescence.
2. device as described in claim 1, wherein the light emitting unit includes:
Light source, for emitting collimated light beam；And
Component is expanded, the exit of the light source is set, for expanding the collimated light beam.
3. device as claimed in claim 2, wherein the component that expands includes the narrowband light filter set gradually and expands
Device, wherein
The narrowband light filter is for filtering to the collimated light beam；
The beam expander is for expanding the light beam after optical filtering.
4. device as described in claim 1, wherein the steering unit is dichroscope.
5. device as described in claim 1, wherein the structure light unit includes:
Digital micro-mirror device；Or
Spatial light modulator；Or
Grating and the driver for controlling the grating movement.
6. device as described in claim 1, wherein described device further includes being arranged in the steering unit downstream to peep list
Member, the interior unit of peeping are used for the beam conduction of steering and focus on sample and receive the fluorescence of sample sending；It is described glimmering
Light is acquired after the steering unit by the face battle array probe unit.
7. device as claimed in claim 6, wherein unit is peeped in described includes coupling object lens and imaging optical fiber bundle, wherein
One end of the imaging optical fiber bundle is arranged in the coupling object lens, for the light beam coupling of the focusing to be entered the light
The proximal end of fine beam；And
The imaging optical fiber bundle is used to conduct the light beam entered.
8. device as claimed in claim 7, wherein peeping unit in described further includes miniature object lens, the miniature object lens setting
In the other end of the imaging optical fiber bundle, the light beam for conducting the fiber optic bundle focuses on the sample.
9. device as described in claim 1, wherein the face battle array probe unit includes the condenser lens set gradually and face battle array
Detector, wherein
The condenser lens is used for the fluorescent foci for issuing the sample；
The planar array detector is for acquiring fluorescence signal.
10. device as claimed in claim 9, wherein the face battle array probe unit further includes long logical optical filter, the logical filter of the length
Light device is arranged between the condenser lens and the planar array detector, for filtering out stray light.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720889338.6U CN208837875U (en) | 2017-07-20 | 2017-07-20 | Tomography endoscopic microscopic imaging device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720889338.6U CN208837875U (en) | 2017-07-20 | 2017-07-20 | Tomography endoscopic microscopic imaging device |
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| CN208837875U true CN208837875U (en) | 2019-05-10 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107361724A (en) * | 2017-07-20 | 2017-11-21 | 南京亘瑞医疗科技有限公司 | Tomography endoscopic microscopic imaging device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107361724A (en) * | 2017-07-20 | 2017-11-21 | 南京亘瑞医疗科技有限公司 | Tomography endoscopic microscopic imaging device |
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Effective date of registration: 20190710 Address after: 215123 B902 room 388, Shui Shui Road, Suzhou Industrial Park, Suzhou, Jiangsu Patentee after: Suzhou micro view medical science and Technology Co., Ltd. Address before: 215123 B902 room 388, Shui Shui Road, Suzhou Industrial Park, Suzhou, Jiangsu Co-patentee before: Nanjing Gen Rui Medical Technology Co Ltd Patentee before: Suzhou micro view medical science and Technology Co., Ltd. |
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