CN107348267B - A kind of health care roxburgh rose beverage and preparation method thereof - Google Patents

A kind of health care roxburgh rose beverage and preparation method thereof Download PDF

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CN107348267B
CN107348267B CN201710723419.3A CN201710723419A CN107348267B CN 107348267 B CN107348267 B CN 107348267B CN 201710723419 A CN201710723419 A CN 201710723419A CN 107348267 B CN107348267 B CN 107348267B
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roxburgh rose
rosa roxburghii
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CN107348267A (en
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李克响
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Guizhou Hengli Natural Biological Science And Technology Co Ltd
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/02Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation containing fruit or vegetable juices
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52Adding ingredients
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/70Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
    • A23L2/84Clarifying or fining of non-alcoholic beverages; Removing unwanted matter using microorganisms or biological material, e.g. enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105Plant extracts, their artificial duplicates or their derivatives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/40Colouring or decolouring of foods
    • A23L5/41Retaining or modifying natural colour by use of additives, e.g. optical brighteners
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Polymers & Plastics (AREA)
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Abstract

The present invention provides a kind of health care roxburgh rose beverage and preparation method thereof, the health care roxburgh rose beverage is made of Rosa roxburghii Tratt, dandelion, sweet osmanthus, hawthorn, glutinous rice, wheat bran, concentration of 0.6% soda ash, yeast, tannase, citric acid and purified water.Health care roxburgh rose beverage of the present invention is high-quality, soup look bright clear, is not easy oxidizing brown stain, nutritional ingredient is balanced, in good taste, no astringent taste.Also with building up health, improving immunity of organisms the effect of.

Description

A kind of health care roxburgh rose beverage and preparation method thereof
Technical field
The present invention relates to a kind of roxburgh rose beverage and preparation method thereof, especially a kind of health care roxburgh rose beverage and its production side Method.
Background technique
With the improvement of living standards, people are increasing to the demand of beverage, in the personal lifestyle of people, on dining table It all can't do without various beverages, such as various sodas, fruit juice, dairy beverage, alkaline drink meet people to each The demand of kind taste.With the increase of competitiveness of society, the operating pressure of people is also increasing, and various drinks are also gradually Birth, such as red ox, happy tiger, add treasured.And with the improvement of living standards, people to the quality requirement of beverage also increasingly Height is also important to note that beverage to the importance of health, and the requirement to the selection of beverage is higher and higher, not only requires mouthfeel It is good, and healthcare function is required, therefore health drink has become the hot spot of current beverage market.
The novel industry that health drink just grew up in recent years in China, still in primary developing stage, development speed It is fast to have the very big rising space.The more scientific call of health drink is functional beverage, and drinks refer to by adjusting beverage The ingredient and content ratio of middle natural nutrient, to adapt to the drink of certain special population nutritional needs, including nutrients beverage, Sports drink and other specific use beverage three classes.Compared with traditional pure water, health drink has in good taste, relieving summer-heat, has Nutrition, promote the production of body fluid to quench thirst strong effect, and than once, much more popular soda more quenches the thirst, is healthier, more tasty and more refreshing, more refreshing oneself, and is expected into For following beverage leading role.
Rosa roxburghii Tratt is that a kind of rosaceous plant belongs to perennial fallen leaves bush, has a large amount of production in Guizhou.Past, the people Between mainly say the fruit of Rosa roxburghii Tratt for helping digestion, antidiarrheal, relieving summer-heat, in " going from place to place seeking official posts notes ", " Chinese medicine dictionary ", Compendium of Material Medica also It is on the books.Rosa roxburghii Tratt also has the function of to adjust body's immunity, anti-aging, removing toxic substances, antiatherosclerosis and antitumor etc.. Peppermint record in " Tang materia medica " " acrid flavour cool in nature, have dispelling wind-heat, refresh oneself, promoting eruption function, dried peppermint leaf enters tea can be with stomach invigorating Wind-dispelling, eliminating the phlegm, cholagogue and other effects.The mood of acceptable gentle nervous indignation can improve spirit, make body and mind joy, help to fall asleep.
In order to develop and use the medical value of Rosa roxburghii Tratt, Rosa roxburghii Tratt is made health care roxburgh rose beverage, promoted to thorn by many producers The exploitation of pears resource.Health care roxburgh rose beverage common at present is mainly roxburgh rose juice drink, it is carried out using pressure extracting juice method Processing, remains most of nutritional ingredient of fruit of Grossularia burejensis Berger, but fruit juice is muddy, impurity content is high, and in deep-processing process Oxidizable brown stain, causes vitamin C to lose, and color is dimmed, so that the nutritional ingredient in beverage is unbalance, leads to trophic structure It changes.Due also to there is stronger astringent taste, influence fruit juice product containing polyphenol substances such as a large amount of tannin in Blackthorn pear fruit-juice Quality and mouthfeel.
Therefore, existing health care roxburgh rose beverage is there are fruit juice muddiness, impurity content height, oxidizable brown stain, and Vc stability is poor, battalion The problems such as forming point unbalance, quality and poor taste.
Summary of the invention
The object of the present invention is to provide a kind of production method of health care roxburgh rose beverage, health care roxburgh rose beverage matter of the present invention It measures, soup look bright clear, is not easy oxidizing brown stain, Vc stability is good, and nutritional ingredient is balanced, in good taste, no astringent taste.Also have and increases The effect of strong constitution, raising immunity of organisms.
In order to solve the above technical problems, the present invention is implemented with the following technical solutions: a kind of health care roxburgh rose beverage, by by weight Measure 30-40 parts of Rosa roxburghii Tratt, 13-19 parts of the dandelion, 12-18 parts of sweet osmanthus, 13-17 parts of hawthorn, 20-30 parts of glutinous rice, wheat bran that part calculates 5-15 parts, 0.5-1.5 parts of the soda ash that concentration is 0.6%, 0.05-0.1 parts of yeast, 0.01-0.05 parts of tannase, citric acid 0.1- 0.5 part and purified water 150-200 parts is made.
Health care roxburgh rose beverage above-mentioned, by calculate by weight 33-37 parts of Rosa roxburghii Tratt, 15-17 parts of dandelion, sweet osmanthus 14- 16 parts, 14-16 parts of hawthorn, 23-27 parts of glutinous rice, 8-12 parts of wheat bran, 0.8-1.2 parts of the soda ash that concentration is 0.6%, yeast 0.06- 0.09 part, 0.02-0.04 parts of tannase, 0.2-0.4 parts of citric acid and 160-190 parts of purified water are made.
Health care roxburgh rose beverage above-mentioned, by calculate by weight 35 parts of Rosa roxburghii Tratt, 16 parts of dandelion, 15 parts of sweet osmanthus, hawthorn 15 parts, 25 parts of glutinous rice, 10 parts of wheat bran, 1.0 parts of the soda ash that concentration is 0.6%, 0.08 part of yeast, 0.03 part of tannase, citric acid 0.3 part and 170 parts of purified water is made.
A kind of health care roxburgh rose beverage above-mentioned, comprising the following steps:
(1) glutinous rice after draining away the water, after boiling 2-3h, naturally cooled to 20-25 with cold water soak 7-9 hours of 0-4 DEG C DEG C A product;
(2) soda ash aqueous solution is added in wheat bran, after mixing, boiling 2-3h under normal pressure obtains B product;
(3) yeast is added in B product, after stirring evenly, places 6-8h at 30-35 DEG C, obtains C product;
(4) C product and A product mix, and place 6-8h at 30-35 DEG C, obtain D product;
(5) D product break into juice, after standing clarification, discard precipitating, supernatant is taken to obtain E product;
(6) Rosa roxburghii Tratt steam distillation extracts volatile oil, obtains F product, collects Rosa roxburghii Tratt slag;
(7) water that Rosa roxburghii Tratt slag is added that 8-12 times is measured extracts 1-3 times, each 1-3h, and filtering, merging filtrate is condensed into concentration It is spare after liquid;Filter residue adds the 6-10 times of ethanol solution for measuring 55-65%, refluxing extraction 2-3 times, and each 0.5-3h is filtered, Merging filtrate recycles ethyl alcohol, obtains alcohol extract, alcohol extract merges with above-mentioned Aqueous extracts, obtains G product;
(8) dandelion, sweet osmanthus and hawthorn are taken, 7-12 times of water is added to decoct 2-4 times, 1-3 hours each, filtration, merging filtrate, Obtain H product;
(9) after E product, G product and H product mix, after being heated to boiling, keep 10-20 minutes slightly boiled, 120 mesh mistakes of extracting solution Filter, filtrate stand 55-65min, take supernatant to filter by 5u filter cloth, filtered supernatant is pumped into material-compound tank, while stirring It mixes side and tannase, citric acid and F product is added, continue to stir 5-10min after adding, add purified water, use 1U after stirring and evenly mixing Filter cloth filtering, sterilizing, it is canned to get.
Health care roxburgh rose beverage above-mentioned, specifically includes the following steps:
(1) glutinous rice after draining away the water, after boiling 2.5h, naturally cooled to 20-25 DEG C with cold water soak 8 hours of 0-4 DEG C Obtain A product;
(2) soda ash aqueous solution is added in wheat bran, after mixing, boiling 2.5h under normal pressure obtains B product;
(3) yeast is added in B product, after stirring evenly, places 7h at 30-35 DEG C, obtains C product;
(4) C product and A product mix, and place 7h at 30-35 DEG C, obtain D product;
(5) D product break into juice, after standing clarification, discard precipitating, supernatant is taken to obtain E product;
(6) Rosa roxburghii Tratt steam distillation extracts volatile oil, obtains F product, collects Rosa roxburghii Tratt slag;
(7) water that 10 times of amounts are added in Rosa roxburghii Tratt slag extracts 2 times, each 2h, filtering, and merging filtrate is standby after being condensed into concentrate With;Filter residue adds the ethanol solution of 8 times of amounts 60%, and refluxing extraction 3 times, each 2h, filtering, merging filtrate recycles ethyl alcohol, obtains Alcohol extract, alcohol extract merge with above-mentioned Aqueous extracts, obtain G product;
(8) dandelion, sweet osmanthus and hawthorn are taken, adds 10 times of water to decoct 3 times, 2 hours every time, filtration, merging filtrate obtained H product;
(9) it after E product, G product and H product mix, after being heated to boiling, is kept for slightly boiled 15 minutes, 120 mesh screens of extracting solution, Filtrate stand 60min, take supernatant to filter by 5u filter cloth, filtered supernatant is pumped into material-compound tank, while stirring plus Enter tannase, citric acid and F product, continues to stir 8min after adding, add purified water, 1U filter cloth mistake is used after stirring and evenly mixing Filter, sterilizing, it is canned to get.
Health care roxburgh rose beverage of the present invention is made of Rosa roxburghii Tratt, dandelion, sweet osmanthus, hawthorn, glutinous rice and wheat bran.Wherein, Rosa roxburghii Tratt (Rosa roxburghii) is the fruit of the perennial machaka rosa roxburghii of rosaceae, is the nutrition treasure fruit of healthcare, is one The rare fruit of kind.The medical value of Rosa roxburghii Tratt is very high, and flower, leaf fruit, seed can be used as medicine, and has stomach invigorating, helps digestion, nourishes, the function of antidiarrheal Effect.Especially Rosa roxburghii Tratt is rich in superoxide dismutase (abbreviation SOD), and SOD is internationally recognized with anti-ageing, protective effect on cancer risk activity Substance, also have the function of it is antiviral, anti-radiation, in terms of cardiovascular, digestive system and various tumor disease prevention and treatments, using ten Divide extensive.Dandelion (Classification system: Taraxacum mongolicum Hand.-Mazz.) composite family contains in dandelion plant There are a variety of health-nutrition ingredients such as taraxol, taraxacin, choline, organic acid, synanthrin.Nature and flavor are sweet, slight bitter, tremble with fear.Return liver, stomach Through.There are diuresis, laxative, removing jaundice subcutaneous ulcer, cholagogue and other effects.Control heat toxin, carbuncle swells, sore, internal carbuncle, red eye, swell pain, damp and hot, jaundice, small Just leaching drips puckery pain, furuncle swelling toxin, acute mastitis, scrofula, toothache, hot eyes, pharyngalgia, pulmonary abscess, acute appendicitis, jaundice with damp-heat pathogen, puckery pain of heat gonorrhea.Sweet osmanthus It is that many woody habits of Chinese Osmanthuss claim, represents species sweet-scented osmanthus (scientific name: Osmanthus fragrans (Thunb.) Lour.), sweet osmanthus effect: yellowish white, fragrance extract aromatic oil, and osmanthus concrete processed can be used for food, cosmetics, can cake processed It selects, candy, and can make wine.Sweet osmanthus acrid flavour can be used as medicine.It is used as medicine with flower, fruit and root.Autumn picks flowers;Spring fruit picking;The four seasons adopt Root dries respectively.Flower: acrid in flavour and warm in nature.Fruit: acrid, sweet, warm.Root: sweet, micro-puckery is put down.Major function: flower: the broken knot of cold dispelling, preventing phlegm from forming and stopping coughing. For having a toothache, cough and phlegm, menostasis abdominal pain.Fruit: warm stomach, calming the liver, cold dispelling.For cold of insufficiency type and stomachache.Root: wind-damp dispelling, cold dispelling.For Rheumatism arthralgia and myalgia, pain in the loins, kidney deficiency toothache.Hawthorn (Crataegus pinnatifida Bunge), have reducing blood lipid, blood pressure, The effects of heart tonifying, arrhythmia, while being also the good medicine of spleen benefiting and stimulating the appetite, relieving dyspepsia, phlegm reduction of blood circulation promoting, the full, hernia to chest diaphragm spleen The diseases such as gas, blood stasis, amenorrhoea have good curative effect.Flavone compound Vitexin in hawthorn is that a kind of antitumaous effect is stronger Drug, extract have certain effect to the internal growth of cancer cells of inhibition, proliferation and infiltration metastasis.Glutinous rice is glutinous rice shelling Rice, glutinous rice contains protein, fat, carbohydrate, calcium, phosphorus, iron, vitamin B1, vitamin B2, niacin and starch etc., and nutrition is rich Richness is the strong food of temperature compensation, has tonifying middle-Jiao and Qi, strengthening spleen and nourishing stomach, only the effect of abnormal sweating, to loss of appetite, abdominal distension diarrhea has one Determine relaxation effect.Glutinous rice is a kind of mild invigorant, have qi-restoratives, enrich blood, invigorating the spleen warm stomach, hidroschesis the effects of.Suitable for taste The diseases such as sweat caused by gastric disorder causing nausea caused by the cold of insufficiency type, poor appetite, diarrhea and the deficiency of vital energy is empty, powerless, the pregnant abdomen falling inflation of shortness of breath.Wheat bran is small Wheat outermost epidermis after wheat is machined by flour milling, becomes flour and wheat bran two parts, wheat bran is exactly the crust of wheat, interior Clothes can strengthening the spleen and stomach, external application make popular with vinegar external application can treat soft tissue injury.
Applicant has carried out long-term a large amount of research to the manufacture craft of roxburgh rose beverage, in the present invention, by wheat bran and pure After aqueous alkali aqueous solution mixes, then yeast is added in boiling, mixed after 30-35 DEG C of heat preservation, then with glutinous rice after boiling and sorghum It is even, it is kept the temperature after mixing then at 30-35 DEG C, the mouthfeel of glutinous rice and sorghum can be provided in this way, so that the beverage taste being finally made It is delicious, and after treated sorghum and glutinous rice reach slurry in this way, slurries are limpid bright, and the slurries have light sweet taste, make The beverage mouthfeel that must be finally made is good, moreover it is possible to the further tart flavour of desalination Rosa roxburghii Tratt.Meanwhile by the method for the present invention to Rosa roxburghii Tratt at Reason, i.e. Rosa roxburghii Tratt steam distillation extract volatile oil, collect Rosa roxburghii Tratt slag;The 8-12 times of water measured extraction 1-3 times is added in Rosa roxburghii Tratt slag, every time 1-3h, filtering, merging filtrate are spare after being condensed into concentrate;Filter residue adds the 6-10 times of ethanol solution for measuring 55-65%, returns Stream extracts 2-3 times, each 0.5-3h, filtering, and merging filtrate recycles ethyl alcohol, obtains alcohol extract, and alcohol extract and above-mentioned Aqueous extracts close And when handling in this way Rosa roxburghii Tratt, the dissolution rate of effective component in Rosa roxburghii Tratt can be improved, so that the roxburgh rose beverage battalion being finally made It supports abundant.Further, since the oxidizing brown stain of the ingredients such as tannin, Vc can add because of the catalysis of heavy metal ion in Rosa roxburghii Tratt extracting solution By force, the citric acid being added in the present invention can then synthesize stable complex with iron ion isochela, be also beneficial to prevention and treatment Rosa roxburghii Tratt The brown stain of fruit juice.Applicant has also carried out experiment investigation to roxburgh rose beverage of the present invention, and part is as follows:
Experimental example 1: character observation
1, sensory evaluation
1.1 take roxburgh rose beverage of the present invention (to be made) test sample by embodiment 1, please receive organoleptic examination training 10 Majors of Food compositions of personnel evaluate group, according to the subjective appreciation standard of table 1, to the fragrance of roxburgh rose beverage, soup look and mouth Sense carries out comprehensive score, selects average mark as final sensory evaluation scores.
1 sensory evaluation criteria of table
1.2 result
It is shown in Table 2.
Table 2
It can be seen from the above result that beverage of the present invention is from fragrance, clarity and mouthfeel, all by the favor of assessment person.
2, clarity is tested
The roxburgh rose beverage of the present invention (being made by implementation 1) for taking 6 parts of encapsulatings good in (4 ± 1 DEG C) refrigerator as refrigerating, and 15 It is taken out after it, centrifugation (4000r/min) handles 10~15min.6 portions of beverages generate deducibility present invention thorn without muddy or precipitating Pear beverage clarity meets the requirements.
2. health-care efficacy of embodiment is investigated
1 materials and methods
1.1 main agents
ConA (Sigm a Products);3H-T dR (China Atomic Energy Science Research Institute);1640 culture medium of RPM I (U.S. G IBOO).
1.2 investigate project: roxburgh rose beverage of the present invention is prepared by embodiment 1.
1.3 animals and cell strain
6-8 week old healthy mice, male, weight 20g ± 2g are provided by animal housing of the court;L929Cell, CTLI-2 cell And YAC-1 cell, vesicular stomatitis virus (VSV) are all provided by Zhejiang Medical university immunology teaching and research room.
1.4 method
1.4.1 animal packet and administration
Animal conventinal breeding.Experimental group (n=10) continuous gavage roxburgh rose beverage of the present invention 5 days, 0.5m l/ mouse/mouth;Stop 5 Day;Again every stomach-filling, continuous 10 days.Control group (n=10) is with method to distilled water.
1.4.2 3The preparation of the YAC-1 cell of H-TdR label
YAC-1 cell fresh cultured object is taken, adjustment cell concentration is 1 × 106Cells/mL, every milliliter of cell add3H- TdR10uCi, in 37 DEG C, 5%CO2Under the conditions of label 3 hours, sufficiently washing after be made into l × 105Ce lls/mL is thin as target Born of the same parents.
1.4.3 mouse boosting cell NK determination of activity
Cervical dislocation puts to death mouse, sterile to take spleen that single cell suspension is made.After no calcium, magnesium Hanks liquid wash twice, with It is 5 × 10 that 11640 complete culture solution of RPM, which adjusts cell concentration,6Cells/mL, as effector cell.On 96 orifice plates, every hole Adding 100 microlitres of concentration is 1 × 105The splenocyte (E:T=50:1) of Cells/mL, control group is with RPM I1640 complete culture solution Substitution effects cell.If 3 multiple holes, in 37 DEG C, 5%CO2Under the conditions of cultivate 18 hours, with bull cell harvester cell In in 49 type glass fiber filter papers.Liquid flashing determining CPM value after drying.N K activity is calculated as follows.As a result to kill percentage table Show.
N K activity=(1- experimental group CPM value/control group CPM value) * 100%.
1.4.4 cell factor induces
Splenocyte is tuned into 1 × 10 with RPM II640 complete culture solution7Cells/mL, on 24 orifice plates, every hole adds 2 millis Rise the liquid, then plus ConA to final concentration 10ug/m1,37 DEG C, 5%CO2Under the conditions of cultivate, respectively at for 24 hours, in 48h, 72h receipts Clearly.- 20 DEG C freeze, and induce horizontal measurement for IL-2 and TNF-beta.
IL-2, TNF-beta and IFN-γ, which induce horizontal measurement result, to be indicated respectively with CPM and killing percentage.
1.4.6 statistical disposition
T is examined, and has the standard of conspicuousness using P < 0.05 as difference.
2 results
The influence of 2.1 pairs of including property of mouse boosting cell NK
It the results are shown in Table 3., it is seen then that roxburgh rose beverage of the present invention can enhance mouse boosting cell NK activity, experimental group and control group phase Than significant difference (P < 0.05).
2.2 couples of IL-2 and TNF-beta induce horizontal influence
It the results are shown in Table 4, it is seen then that test group very significant (P < 0.05) test group splenocyte of difference compared with the control group.
Influence of the table 3 to including property of mouse boosting cell NK
Note: compared with the control group,*P<0.05。
Table 4 induces horizontal influence to IL-2, TNF-beta
Note: compared with the control group,*P<0.05。
It follows that roxburgh rose beverage of the present invention, which can significantly increase splenocyte NK activity, IL-2 and TNF-beta, induces level, because And enhance mouse cell immune function.
3. brown stain of experimental example and Vc stability study
(1) brown stain is studied
1 detection project
1.1 roxburgh rose beverages of the present invention: it is prepared by embodiment;
1.2 comparison roxburgh rose beverages 1: it is prepared as follows:
(1) it selects bluish yellow color, eight or nine to be divided into ripe fruit of Grossularia burejensis Berger, cleans, obtain A product;
(2) A product squeeze, when fruit juice outflow when, stop squeeze 3min, be further continued for squeezing, be repeated, until no fruit juice outflow, Fruit juice is collected, B product are obtained;
(3) by being passed through CuSO in every liter of B product4, seal, stand 6 days, obtain C product;CuSO4Weight ratio with B product is 1: 20;
(4) gelatin is taken, 1-10 DEG C of the pure water measured with 4 times impregnates 10h, and decontamination is gone out after expansion, and 7 times of amounts are added 60-65 DEG C of water obtains D product;
(5) D product are slowly added in C product, stirring while adding, continue to stir 8min after adding, and seal oxygen barrier standing 16 days, Obtain E product;
(6) vacuum outgas 100min, devolatilization vacuum degree are 0.097MPa to E product at normal temperature, obtain F product;
(7) F product sterilization, it is canned to get.
1.3 comparison roxburgh rose beverages 2: commercially available: the date of manufacture is as the preparation date for comparing roxburgh rose beverage of the present invention.
2 test methods
Roxburgh rose beverage, comparison roxburgh rose beverage 1 and the comparison each 10mL of roxburgh rose beverage 2 of the present invention are taken, is placed in Brown Glass Brown glass bottles and jars only, Sealing, it is In Shade, every 15 days, 1 month, 2 months, 3 months, 4 months, observe within 5 months and 6 months the color of each beverage Variation.Specifically it is shown in Table 5.
Table 5
As seen from table, roxburgh rose beverage of the present invention is not easy brown stain, and quality is best.
(2) Vc stability study
1 detection project
1.1 roxburgh rose beverages of the present invention: it is prepared by embodiment;
1.2 comparison roxburgh rose beverages 1: it is prepared as follows:
(1) it selects bluish yellow color, eight or nine to be divided into ripe fruit of Grossularia burejensis Berger, cleans, obtain A product;
(2) A product squeeze, when fruit juice outflow when, stop squeeze 3min, be further continued for squeezing, be repeated, until no fruit juice outflow, Fruit juice is collected, B product are obtained;
(3) by being passed through CuSO in every liter of B product4, seal, stand 6 days, obtain C product;CuSO4Weight ratio with B product is 1: 20;
(4) gelatin is taken, 1-10 DEG C of the pure water measured with 4 times impregnates 10h, and decontamination is gone out after expansion, and 7 times of amounts are added 60-65 DEG C of water obtains D product;
(5) D product are slowly added in C product, stirring while adding, continue to stir 8min after adding, and seal oxygen barrier standing 16 days, Obtain E product;
(6) vacuum outgas 100min, devolatilization vacuum degree are 0.097MPa to E product at normal temperature, obtain F product;
(7) F product sterilization, it is canned to get.
1.3 comparison roxburgh rose beverages 2: commercially available: the date of manufacture is as the preparation date for comparing roxburgh rose beverage of the present invention.
2 test methods
Roxburgh rose beverage of the present invention, comparison roxburgh rose beverage 1 and comparison 2 each 6 parts of roxburgh rose beverage, every part of 10mL are taken, each thorn is measured The Vc content results of the Vc content of pear beverage, various roxburgh rose beverages are averaged, and are obtained the initial Vc content of each beverage, are subsequently placed in In Brown Glass Brown glass bottles and jars only, sealing, (4 DEG C) period color variations of low tempertaure storage are In Shade, in each roxburgh rose beverage after 4 months Vc content is detected, and the Vc content results of various roxburgh rose beverages are averaged, and obtains the final Vc content value of each beverage, calculates Vc Loss late.Specifically it is shown in Table 6.The wherein loss late of Vc=(the final Vc content of initial Vc content -)/initial Vc content * 100%.
Table 6
Detection project Loss late/% of Vc
Roxburgh rose beverage of the present invention 3.9%
Compare roxburgh rose beverage 1 13.7
Compare roxburgh rose beverage 2 16.9
As seen from table, roxburgh rose beverage Vc loss late of the present invention is minimum, and Vc stability is best, and quality is best.
Compared with prior art, health care roxburgh rose beverage of the present invention is high-quality, and soup look bright clear is not easy oxidizing brown stain, Vc Stability is good, and nutritional ingredient is balanced, in good taste, no astringent taste.Also with building up health, improving immunity of organisms the effect of.
Specific embodiment
Embodiment 1:
Health care roxburgh rose beverage formula: Rosa roxburghii Tratt 35kg, dandelion 16kg, sweet osmanthus 15kg, hawthorn 15kg, glutinous rice 25kg, wheat bran 10kg, soda ash 1.0kg, yeast 0.08kg, tannase 0.03kg, citric acid 0.3kg and the purified water 170kg that concentration is 0.6%.
Health care roxburgh rose beverage manufacture craft: specifically includes the following steps:
(1) glutinous rice after draining away the water, after boiling 2.5h, naturally cooled to 20-25 DEG C with cold water soak 8 hours of 0-4 DEG C Obtain A product;
(2) soda ash aqueous solution is added in wheat bran, after mixing, boiling 2.5h under normal pressure obtains B product;
(3) yeast is added in B product, after stirring evenly, places 7h at 30-35 DEG C, obtains C product;
(4) C product and A product mix, and place 7h at 30-35 DEG C, obtain D product;
(5) D product break into juice, after standing clarification, discard precipitating, supernatant is taken to obtain E product;
(6) Rosa roxburghii Tratt steam distillation extracts volatile oil, obtains F product, collects Rosa roxburghii Tratt slag;
(7) water that 10 times of amounts are added in Rosa roxburghii Tratt slag extracts 2 times, each 2h, filtering, and merging filtrate is standby after being condensed into concentrate With;Filter residue adds the ethanol solution of 8 times of amounts 60%, and refluxing extraction 3 times, each 2h, filtering, merging filtrate recycles ethyl alcohol, obtains Alcohol extract, alcohol extract merge with above-mentioned Aqueous extracts, obtain G product;
(8) dandelion, sweet osmanthus and hawthorn are taken, adds 10 times of water to decoct 3 times, 2 hours every time, filtration, merging filtrate obtained H product;
(9) it after E product, G product and H product mix, after being heated to boiling, is kept for slightly boiled 15 minutes, 120 mesh screens of extracting solution, Filtrate stand 60min, take supernatant to filter by 5u filter cloth, filtered supernatant is pumped into material-compound tank, while stirring plus Enter tannase, citric acid and F product, continues to stir 8min after adding, add purified water, 1U filter cloth mistake is used after stirring and evenly mixing Filter, sterilizing are canned to get health drink.
Embodiment 2.
Health care roxburgh rose beverage: Rosa roxburghii Tratt 37kg, dandelion 17kg, sweet osmanthus 16kg, hawthorn 16kg, glutinous rice 27kg, wheat bran 12kg, Soda ash 1.2kg, yeast 0.09kg, tannase 0.04kg, citric acid 0.4kg and the purified water 190kg that concentration is 0.6%.
Health care roxburgh rose beverage manufacture craft:
(1) glutinous rice after being drained away the water, after boiling 3h, was naturally cooled to 20-25 DEG C and obtained with cold water soak 9 hours of 0-4 DEG C A product;
(2) soda ash aqueous solution is added in wheat bran, after mixing, boiling 3h under normal pressure obtains B product;
(3) yeast is added in B product, after stirring evenly, places 8h at 30-35 DEG C, obtains C product;
(4) C product and A product mix, and place 8h at 30-35 DEG C, obtain D product;
(5) D product break into juice, after standing clarification, discard precipitating, supernatant is taken to obtain E product;
(6) Rosa roxburghii Tratt steam distillation extracts volatile oil, obtains F product, collects Rosa roxburghii Tratt slag;
(7) water that 12 times of amounts are added in Rosa roxburghii Tratt slag extracts 3 times, each 3h, filtering, and merging filtrate is standby after being condensed into concentrate With;Filter residue adds the ethanol solution of 10 times of amounts 65%, and refluxing extraction 3 times, each 3h, filtering, merging filtrate recycles ethyl alcohol, Alcohol extract is obtained, alcohol extract merges with above-mentioned Aqueous extracts, obtains G product;
(8) dandelion, sweet osmanthus and hawthorn are taken, adds 12 times of water to decoct 4 times, 3 hours every time, filtration, merging filtrate obtained H product;
(9) it after E product, G product and H product mix, after being heated to boiling, is kept for slightly boiled 20 minutes, 120 mesh screens of extracting solution, Filtrate stand 65min, take supernatant to filter by 5u filter cloth, filtered supernatant is pumped into material-compound tank, while stirring plus Enter tannase, citric acid and F product, continues to stir 10min after adding, add purified water, 1U filter cloth mistake is used after stirring and evenly mixing Filter, sterilizing, it is canned to get.
Embodiment 3.
Health care roxburgh rose beverage: Rosa roxburghii Tratt 33kg, dandelion 15kg, sweet osmanthus 14kg, hawthorn 14kg, glutinous rice 23kg, wheat bran 8kg, Soda ash 0.8kg, yeast 0.06kg, tannase 0.02kg, citric acid 0.2kg and the purified water 160kg that concentration is 0.6%.
Health care roxburgh rose beverage manufacture craft:
(1) glutinous rice after being drained away the water, after boiling 2h, was naturally cooled to 20-25 DEG C and obtained with cold water soak 7 hours of 0-4 DEG C A product;
(2) soda ash aqueous solution is added in wheat bran, after mixing, boiling 2h under normal pressure obtains B product;
(3) yeast is added in B product, after stirring evenly, places 6h at 30-35 DEG C, obtains C product;
(4) C product and A product mix, and place 6h at 30-35 DEG C, obtain D product;
(5) D product break into juice, after standing clarification, discard precipitating, supernatant is taken to obtain E product;
(6) Rosa roxburghii Tratt steam distillation extracts volatile oil, obtains F product, collects Rosa roxburghii Tratt slag;
(7) water that 8 times of amounts are added in Rosa roxburghii Tratt slag extracts 1 time, each 1h, filtering, and merging filtrate is standby after being condensed into concentrate With;Filter residue adds the ethanol solution of 6 times of amounts 55%, and refluxing extraction 2 times, each 0.5h, filtering, merging filtrate recycles ethyl alcohol, Alcohol extract is obtained, alcohol extract merges with above-mentioned Aqueous extracts, obtains G product;
(8) dandelion, sweet osmanthus and hawthorn are taken, adds 7 times of water to decoct 2 times, 1 hour every time, filtration, merging filtrate obtained H product;
(9) it after E product, G product and H product mix, after being heated to boiling, is kept for slightly boiled 10 minutes, 120 mesh screens of extracting solution, Filtrate stand 55min, take supernatant to filter by 5u filter cloth, filtered supernatant is pumped into material-compound tank, while stirring plus Enter tannase, citric acid and F product, continues to stir 5min after adding, add purified water, 1U filter cloth mistake is used after stirring and evenly mixing Filter, sterilizing, it is canned to get.

Claims (3)

1. a kind of health care roxburgh rose beverage, it is characterised in that: by calculate by weight 33-37 parts of Rosa roxburghii Tratt, 15-17 parts of dandelion, 14-16 parts of sweet osmanthus, 14-16 parts of hawthorn, 23-27 parts of glutinous rice, 8-12 parts of wheat bran, 0.8-1.2 parts of the soda ash that concentration is 0.6%, yeast 0.06-0.09 parts, 0.02-0.04 parts of tannase, 0.2-0.4 parts of citric acid and 160-190 parts of purified water are made;
The preparation method of the health care roxburgh rose beverage, comprising the following steps:
(1) glutinous rice after draining away the water, after boiling 2-3h, naturally cooled to 20-25 DEG C with cold water soak 7-9 hours of 0-4 DEG C Obtain A product;
(2) soda ash aqueous solution is added in wheat bran, after mixing, boiling 2-3h under normal pressure obtains B product;
(3) yeast is added in B product, after stirring evenly, places 6-8h at 30-35 DEG C, obtains C product;
(4) C product and A product mix, and place 6-8h at 30-35 DEG C, obtain D product;
(5) D product break into juice, after standing clarification, discard precipitating, supernatant is taken to obtain E product;
(6) Rosa roxburghii Tratt steam distillation extracts volatile oil, obtains F product, collects Rosa roxburghii Tratt slag;
(7) water that Rosa roxburghii Tratt slag is added that 8-12 times is measured extracts 1-3 times, each 1-3h, and filtering, merging filtrate is condensed into concentrate Afterwards, spare;Filter residue adds the 6-10 times of ethanol solution for measuring 55-65%, refluxing extraction 2-3 times, and each 0.5-3h is filtered, and merges Filtrate recycles ethyl alcohol, obtains alcohol extract, alcohol extract merges with above-mentioned Aqueous extracts, obtains G product;
(8) dandelion, sweet osmanthus and hawthorn are taken, 7-12 times of water is added to decoct 2-4 times, 1-3 hours each, filtration, merging filtrate obtains H Product;
(9) after E product, G product and H product mix, after being heated to boiling, keep 10-20 minutes slightly boiled, 120 mesh screens of extracting solution, Filtrate stands 55-65min, takes supernatant to filter by 5u filter cloth, filtered supernatant is pumped into material-compound tank, while stirring Tannase, citric acid and F product are added, continues to stir 5-10min after adding, adds purified water, filtered after stirring and evenly mixing with 1U Cloth filtering, sterilizing, it is canned to get.
2. health care roxburgh rose beverage as described in claim 1, it is characterised in that: by 35 parts of Rosa roxburghii Tratt, the Pu Gong calculated by weight 16 parts of English, 15 parts of sweet osmanthus, 15 parts of hawthorn, 25 parts of glutinous rice, 10 parts of wheat bran, concentration be 0.6% 1.0 parts of soda ash, 0.08 part of yeast, 0.03 part of tannase, 0.3 part of citric acid and 170 parts of purified water are made.
3. health care roxburgh rose beverage as described in claim 1, it is characterised in that: the preparation method of the roxburgh rose beverage is specific to wrap Include following steps:
(1) glutinous rice after being drained away the water, after boiling 2.5h, was naturally cooled to 20-25 DEG C and obtains A with cold water soak 8 hours of 0-4 DEG C Product;
(2) soda ash aqueous solution is added in wheat bran, after mixing, boiling 2.5h under normal pressure obtains B product;
(3) yeast is added in B product, after stirring evenly, places 7h at 30-35 DEG C, obtains C product;
(4) C product and A product mix, and place 7h at 30-35 DEG C, obtain D product;
(5) D product break into juice, after standing clarification, discard precipitating, supernatant is taken to obtain E product;
(6) Rosa roxburghii Tratt steam distillation extracts volatile oil, obtains F product, collects Rosa roxburghii Tratt slag;
(7) water that 10 times of amounts are added in Rosa roxburghii Tratt slag extracts 2 times, each 2h, filtering, and merging filtrate is spare after being condensed into concentrate; Filter residue adds the ethanol solution of 8 times of amounts 60%, and refluxing extraction 3 times, each 2h, filtering, merging filtrate recycles ethyl alcohol, obtains alcohol extracting Liquid is taken, alcohol extract merges with above-mentioned Aqueous extracts, obtains G product;
(8) dandelion, sweet osmanthus and hawthorn are taken, adds 10 times of water to decoct 3 times, 2 hours every time, filtration, merging filtrate obtained H product;
(9) it after E product, G product and H product mix, after being heated to boiling, is kept for slightly boiled 15 minutes, extracting solution is with 120 mesh screens, filtrate 60min is stood, supernatant is taken to filter by 5u filter cloth, filtered supernatant is pumped into material-compound tank, is added while stirring single Peaceful enzyme, citric acid and F product continue to stir 8min after adding, add purified water, filtered, gone out with 1U filter cloth after stirring and evenly mixing Bacterium, it is canned to get.
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CN101012425A (en) * 2007-02-01 2007-08-08 杨安顺 Method of brewing rosa roxburghii glutinous rice wine
CN101040694A (en) * 2007-04-28 2007-09-26 王晓阳 Multi-flavour beverage of rosa roxburghii tratt and balsam pear
CN102204702A (en) * 2011-04-27 2011-10-05 武汉胜发机电研究所 Method for preparing rosa roxburghii tratt and orange juice beverage
CN102972814A (en) * 2011-09-07 2013-03-20 米兴才 Roxburgh rose juice drink
CN104026690B (en) * 2014-06-23 2016-04-06 贵阳高新创嘉创业服务有限公司 A kind of Cili Juice and preparation method thereof
CN104560518A (en) * 2015-01-16 2015-04-29 贵州省轻工业科学研究所 Production method of compound functional beverage of seedless thorn pears and fermented glutinous rice
CN105250340B (en) * 2015-10-08 2018-11-30 河南省中医药研究院 A kind of preparation method and applications of father-in-law's cloth extract

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