CN107347641A - A kind of paper mulberry tissue culture nutrient solution - Google Patents
A kind of paper mulberry tissue culture nutrient solution Download PDFInfo
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- CN107347641A CN107347641A CN201710609824.2A CN201710609824A CN107347641A CN 107347641 A CN107347641 A CN 107347641A CN 201710609824 A CN201710609824 A CN 201710609824A CN 107347641 A CN107347641 A CN 107347641A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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Abstract
The invention belongs to biological technical field, more particularly to a kind of paper mulberry tissue culture nutrient solution, is prepared by mother liquor A, mother liquor B, mother solution C, mother liquor D, mother liquor E, plant growth regulator, sucrose, agar powder, oxidation micro silver powder, activated carbon micro mist；Contain CaCl in mother liquor A2·2H2O、KNO3、MgSO4·7H2O、NH4NO3And KH2PO4；Contain MgSO in mother liquor B4·4H2O、ZnSO4·7H2O、H3BO3、KI、NeMoO4·2H2O、CuSO4·5H2O and CoCl6H2O；Containing thiamine, pyridoxol, Nicotinic it is in mother solution C and glycine；Mother liquor D is inositol solution；Contain FeSO in mother liquor E4·7H2O and Na2‑EDTA.Activated carbon micro mist and oxidation micro silver powder are added in nutrient solution, activated carbon produces synergy with silver oxide, the generation for situations such as effectively avoiding vitrifying, yellow, browning, variation, low appreciation rate, may advantageously facilitate the rooting rate and transplanting survival rate of paper mulberry tissue cultures.
Description
Technical field
The present invention relates to biological technical field, it particularly relates to a kind of paper mulberry tissue culture nutrient solution.
Background technology
Paper mulberry category Moraceae, it is perennial broadleaf arbor.China the Yellow River, the Changjiang river and Pearl River Delta are distributed mainly on, in Guizhou
Anshun is distributed, mostly wild, there is a small amount of cultivation, belongs to local indigenous tree.With fast-growing, strong adaptability, it is distributed wide, heat
High, the characteristics of period of felling in turn is short.Its bark, timber, branch, leaf, flower, fruit, seed, root, milk etc. can all comprehensively utilize, economical
Benefit potentiality are very big.Paper mulberry whole body can utilize：Bark fiber is pure white, elongated, is papermaking and the textile raw material of top grade, is worth
Height, the huge market demand；Rod is production paper, the good raw material of fiberboard；Leaf contains abundant crude protein, amino acid, micro-
Secondary element, it is the high-quality feed raw material of animal.Paper mulberry, 200 kilograms of per mu yield bast fiber, wood are planted using high-density planting technology
1500 kilograms of bar, 1500 kilograms of high-quality feed leaf.Because paper mulberry has greening and other economic values, market is for paper mulberry kind
The demand of seedling is very big, is up to 20,000,000 plants every year.
At present prepared by the seedling of paper mulberry to supply mainly by cuttage and tissue-cultured seedling.There is seasonal, place occupancy face for cuttage
The problems such as product is big, efficiency is low.There is the problems such as cost of labor is high, the amount of labour is big, cost is high for conventional tissue culture method.In tissue culture mistake
Easily cause tissue culture seedling pollution, material death, vitrifying, yellow, browning, variation, appreciation rate in journey lowly or cross Sheng, tissue-cultured seedling not
Take root or take root thin and weak exception, tissue-cultured seedling or excessive growth, bottle seedling bottle outlet death rate height etc..
The content of the invention
In view of this, it is an object of the invention to provide a kind of paper mulberry tissue culture nutrient solution, to solve existing paper mulberry tissue culture method
Easily cause tissue culture seedling pollution, material death, vitrifying, yellow, browning, variation, appreciation rate lowly or cross Sheng, tissue-cultured seedling is not taken root
Or exception of taking root, tissue-cultured seedling be thin and weak or excessive growth, the problems such as bottle seedling bottle outlet death rate is high.
The present invention solves above-mentioned technical problem by the following technical programs：
A kind of paper mulberry tissue culture nutrient solution, A80 containing mother liquor~120g, mother liquor B0.8~1.2g, mother liquor in every liter of nutrient solution
C0.8~1.2g, mother liquor D8~12g, mother liquor E3~7g, 0.8~1.2g of plant growth regulator, 25~35g of sucrose, agar powder 5
~6g, 3~5g of oxidation micro silver powder, 3~5g of activated carbon micro mist；
CaCl in the mother liquor A2·2H2O concentration is 4~5g/L, KNO3Concentration is 18~20g/L, MgSO4·7H2O concentration
For 3~4g/L, NH4NO3Concentration is 16~17g/L, KH2PO4Concentration is 1~2g/L；
MgSO in the mother liquor B4·4H2O concentration is 24~25g/L, ZnSO4·7H2O concentration is 8~9g/L, H3BO3It is dense
Spend for 6.0~6.5g/L, KI concentration is 0.8~0.9g/L, NeMoO4·2H2O concentration is 0.2~0.3g/L, CuSO4·5H2O
Concentration is 0.02~0.03g/L, CoCl6H2O concentration is 0.02~0.03g/L；
Thiamine concentration is 0.4~0.6g/L in the mother solution C, pyridoxol concentration is 0.3~0.5g/L, Nicotinic is
0.4~0.6g/L, glycine concentration are 1~3g/L；
The mother liquor D is the inositol solution that mass concentration is 8~12g/L；
FeSO in the mother liquor E4·7H2O concentration is 5~6g/L, Na2- EDTA concentration is 7~8g/L.
A100g containing mother liquor, mother liquor B1g, mother solution C 1g, mother liquor D10g, mother liquor E5g, plant growth regulating in the nutrient solution
Agent 1g, sucrose 30g, agar powder 5.5g, oxidation micro silver powder 4g, activated carbon micro mist 4g.
The plant growth regulator is by 6-benzyl aminopurine, methyl α-naphthyl acetate and Lychee juice with (4~7)：(1~2)：(1.5~
2.5) weight ratio is formulated.
The plant growth regulator is by heteroauxin, Vc, Lychee juice with (100~120)：(1~2)：(180~200)
Weight ratio be formulated.
The oxidation micro silver powder is the nano powder of silver oxide.
The activated carbon micro mist is the nano powder of activated carbon.
The compound method of the mother liquor A is：Take CaCl2·2H2O0.4~0.5g is dissolved in 300mL distilled water, takes KNO3
18~20g, MgSO4·7H2O3.5~4g, NH4NO316~17g, KH2PO41.5~2g mixing is dissolved in 500mL distilled water,
After two kinds of solution of acquisition are mixed, then 1L is dissolved to, produces mother liquor A.
The compound method of the mother liquor B is：Take MgSO4·4H2O24~25g, ZnSO4·7H2O8~9g, H3BO36.0~
6.5g, KI0.8~0.9g, NeMoO4·2H2O0.2~0.3g, CuSO4·5H2O0.02~0.03g, CoCl6H2O0.02~
0.03g is dissolved in 800mL distilled water, then is dissolved to 1000mL, produces mother liquor B.
The compound method of the mother solution C is：Take 0.04~0.06g of thiamine, 0.03~0.05g of pyridoxol, nicotinic acid 0.04
~0.06g, 0.1~0.3g of glycine are dissolved in 80mL distilled water, then are dissolved to 100mL, produce mother solution C；
The compound method of the mother liquor E is：Take FeSO4·7H2O2.5~3g is dissolved in 400mL distilled water, adds Na2-
EDTA3.5~4g is heated to 80~120 DEG C of 5~8min of dissolving, then is dissolved to 500mL, produces mother liquor E.
CaCl in the mother liquor A2·2H2O concentration is 4.4g/L, KNO3Concentration is 19g/L, MgSO4·7H2O concentration is
3.8g/L、NH4NO3Concentration is 16.5g/L, KH2PO4Concentration is 1.8g/L.
MgSO in the mother liquor B4·4H2O concentration is 24.3g/L, ZnSO4·7H2O concentration is 8.6g/L, H3BO3Concentration is
6.2g/L, KI concentration are 0.82g/L, NeMoO4·2H2O concentration is 0.25g/L, CuSO4·5H2O concentration be 0.025g/L,
CoCl·6H2O concentration is 0.025g/L.
Thiamine concentration is 0.5g/L in the mother solution C, pyridoxol concentration is 0.4g/L, Nicotinic 0.5g/L, sweet
Propylhomoserin concentration is 2g/L.
The mother liquor D is the inositol solution that mass concentration is 10g/L.
FeSO in the mother liquor E4·7H2O concentration is 5.4g/L, Na2- EDTA concentration is 7.4g/L.
The beneficial effects of the present invention are：First, activated carbon micro mist is added in nutrient solution, polyphenol oxidase can be reduced
Activity, mitigate the incidence of oxidation, can effectively absorb the phenols and quinones harmful substance in nutrient solution, addition oxidation
Micro silver powder helps to decompose the metabolite in culture, and effectively suppresses pollution caused by explant endophyte class, activated carbon
Synergy is produced with silver oxide, the generation for situations such as effectively avoiding vitrifying, yellow, browning, variation, low appreciation rate, is had
Beneficial to the rooting rate and transplanting survival rate for promoting paper mulberry tissue cultures；Secondly, addition Lychee juice promotes the growth of paper mulberry adventitious bud,
Breeding coefficient is high, and root induction while propagation, shortens cultivation period；Again, using the nutrient solution of the present invention, rooting rate
Reach with transplanting survival rate up to more than 99.5%, genetic stability is good.
Embodiment
In order to facilitate the understanding of those skilled in the art, below in conjunction with embodiment, the present invention will be further described.
Embodiment is only for example, not being limitation of the invention, the step of not illustrated in embodiment to the invention
It is prior art, is not described in detail herein.
Embodiment one
A kind of paper mulberry tissue culture nutrient solution, every liter of (L) nutrient solution A80g containing mother liquor, mother liquor B0.8g, mother solution C 0.8g, mother liquor
D8g, mother liquor E3g, plant growth regulator 0.8g, sucrose 25g, agar powder 5g, oxidation micro silver powder 3g, activated carbon micro mist 3g, training
The compound method of nutrient solution is：Take mother liquor A80g, mother liquor B0.8g, mother solution C 0.8g, mother liquor D8g, mother liquor E3g, plant growth regulating
After agent 0.8g, sucrose 25g, agar powder 5g, oxidation micro silver powder 3g, activated carbon micro mist 3g are sufficiently mixed, 1L is dissolved to, is produced.
CaCl in the mother liquor A2·2H2O concentration is 4g/L, KNO3Concentration is 18g/L, MgSO4·7H2O concentration be 3g/L,
NH4NO3Concentration is 16g/L, KH2PO4Concentration is 1g/L；Mother liquor A compound method is：Take CaCl2·2H2O0.4g is dissolved in 300mL
In distilled water, KNO is taken3 18g、MgSO4·7H2O3.5g、NH4NO3 16g、KH2PO41.5g mixing is dissolved in 500mL distilled water
In, when solid matter is not seen in two kinds of solution, after two kinds of solution of acquisition are mixed, then 1L is dissolved to, produces mother liquor A.
MgSO in the mother liquor B4·4H2O concentration is 24g/L, ZnSO4·7H2O concentration is 8g/L, H3BO3Concentration is
6.0g/L, KI concentration are 0.8g/L, NeMoO4·2H2O concentration is 0.2g/L, CuSO4·5H2O concentration is 0.02g/L, CoCl
6H2O concentration is 0.02g/L；The compound method of the mother liquor B is：Take MgSO4·4H2O24g、ZnSO4·7H2O8g、H3BO3
6.0g、KI0.8g、NeMoO4·2H2O0.2g、CuSO4·5H2O0.02g、CoCl·6H2O0.02g is dissolved in 800mL distilled water,
Treat that above-mentioned substance is dissolved completely in distilled water, then be dissolved to 1000mL, produce mother liquor B.
Thiamine concentration is 0.4g/L in the mother solution C, pyridoxol concentration is 0.3g/L, Nicotinic 0.4g/L, sweet
Propylhomoserin concentration is 1g/L；The compound method of the mother solution C is：Take thiamine 0.04g, pyridoxol 0.03g, nicotinic acid 0.04g, sweet ammonia
Sour 0.1g is dissolved in 80mL distilled water, is dissolved to 100mL again after above-mentioned substance is dissolved completely in water, is produced mother solution C.
The mother liquor D is the inositol solution that mass concentration is 8g/L.
FeSO in the mother liquor E4·7H2O concentration is 5g/L, Na2- EDTA concentration is 7g/L.The preparation side of the mother liquor E
Method is：Take FeSO4·7H2O2.5g is dissolved in 400mL distilled water, adds Na2- EDTA3.5g is heated to 80 DEG C of dissolving 5min,
Treat that above-mentioned substance is dissolved completely in water, then be dissolved to 500mL, produce mother liquor E.
The plant growth regulator is by 6-benzyl aminopurine, methyl α-naphthyl acetate and Lychee juice with 4：1：1.5 weight is than preparing
Form, the plant growth regulator is subculture reagent, uses the nutrient solution that the plant growth regulator is prepared as squamous subculture liquid.
The plant growth regulator is by heteroauxin, Vc, Lychee juice with 100：1：180 weight ratio is formulated, should
Plant growth regulator is reagent of taking root, and uses the nutrient solution that the plant growth regulator is prepared as nutrient solution of taking root.
The oxidation micro silver powder is the nano powder of silver oxide.
The activated carbon micro mist is the nano powder of activated carbon.
Embodiment two
A kind of paper mulberry tissue culture nutrient solution, every liter of (L) nutrient solution A120g containing mother liquor, mother liquor B1.2g, mother solution C 1.2g, mother liquor
D12g, mother liquor E7g, plant growth regulator 1.2g, sucrose 35g, agar powder 6g, oxidation micro silver powder 5g, activated carbon micro mist 5g, training
The compound method of nutrient solution is：Mother liquor A120g, mother liquor B1.2g, mother solution C 1.2g, mother liquor D12g, mother liquor E7g, plant growth is taken to adjust
Section agent 1.2g, sucrose 35g, agar powder 6g, oxidation micro silver powder 5g, activated carbon micro mist 5g are well mixed, then are dissolved to 1L, produce.
CaCl in the mother liquor A2·2H2O concentration is 5g/L, KNO3Concentration is 20g/L, MgSO4·7H2O concentration be 4g/L,
NH4NO3Concentration is 17g/L, KH2PO4Concentration is 2g/L；Mother liquor A compound method is：Take CaCl2·2H2O0.5g is dissolved in 300mL
In distilled water, KNO is taken3 20g、MgSO4·7H2O4g、NH4NO3 17g、KH2PO42g mixing is dissolved in 500mL distilled water, is treated
When solid matter is not seen in two kinds of solution, after two kinds of solution of acquisition are mixed, then 1L is dissolved to, produces mother liquor A.
MgSO in the mother liquor B4·4H2O concentration is 25g/L, ZnSO4·7H2O concentration is 9g/L, H3BO3Concentration is
6.5g/L, KI concentration are 0.9g/L, NeMoO4·2H2O concentration is 0.3g/L, CuSO4·5H2O concentration is 0.03g/L, CoCl
6H2O concentration is 0.03g/L；The compound method of the mother liquor B is：Take MgSO4·4H2O25g、ZnSO4·7H2O9g、H3BO3
6.5g、KI0.9g、NeMoO4·2H2O0.3g、CuSO4·5H2O0.03g、CoCl·6H2O0.03g is dissolved in 800mL distilled water,
Treat that above-mentioned substance is dissolved completely in distilled water, then be dissolved to 1000mL, produce mother liquor B.
Thiamine concentration is 0.6g/L in the mother solution C, pyridoxol concentration is 0.5g/L, Nicotinic 0.6g/L, sweet
Propylhomoserin concentration is 3g/L；The compound method of the mother solution C is：Take thiamine 0.06g, pyridoxol 0.05g, nicotinic acid 0.06g, sweet ammonia
Sour 0.3g is dissolved in 80mL distilled water, is dissolved to 100mL again after above-mentioned substance is dissolved completely in water, is produced mother solution C.
The mother liquor D is the inositol solution that mass concentration is 12g/L.
FeSO in the mother liquor E4·7H2O concentration is 6g/L, Na2- EDTA concentration is 8g/L.The preparation side of the mother liquor E
Method is：Take FeSO4·7H2O3g is dissolved in 400mL distilled water, adds Na2- EDTA4g is heated to 120 DEG C of dissolving 8min, treats
State material to be dissolved completely in water, then be dissolved to 500mL, produce mother liquor E.
The plant growth regulator is by 6-benzyl aminopurine, methyl α-naphthyl acetate and Lychee juice with 7：2：2.5 weight is than preparing
Form, the plant growth regulator is subculture reagent, and the nutrient solution for adding plant growth regulator preparation is squamous subculture liquid.
The plant growth regulator is by heteroauxin, Vc, Lychee juice with 120：2：200 weight ratio is formulated, should
Plant growth regulator is reagent of taking root, and the nutrient solution for adding plant growth regulator preparation is nutrient solution of taking root.
The oxidation micro silver powder is the nano powder of silver oxide.
The activated carbon micro mist is the nano powder of activated carbon.
Embodiment three
A kind of paper mulberry tissue culture nutrient solution, every liter of (L) nutrient solution A100g containing mother liquor, mother liquor B1g, mother solution C 1g, mother liquor D10g,
Mother liquor E5g, plant growth regulator 1g, sucrose 30g, agar powder 5.5g, oxidation micro silver powder 4g, activated carbon micro mist 4g, nutrient solution
Compound method be：Take mother liquor A100g, mother liquor B1g, mother solution C 1g, mother liquor D10g, mother liquor E5g, plant growth regulator 1g, sugarcane
Sugared 30g, agar powder 5.5g, oxidation micro silver powder 4g, activated carbon micro mist 4g are well mixed, then are dissolved to 1L, produce.
CaCl in the mother liquor A2·2H2O concentration is 4.4g/L, KNO3Concentration is 19g/L, MgSO4·7H2O concentration is
3.8g/L、NH4NO3Concentration is 16.5g/L, KH2PO4Concentration is 1.8g/L；Mother liquor A compound method is：Take CaCl2·
2H2O0.44g is dissolved in 300mL distilled water, takes KNO3 19g、MgSO4·7H2O3.8g、NH4NO3 16.5g、KH2PO4 1.8g
Mixing is dissolved in 500mL distilled water, when solid matter is not seen in two kinds of solution, after two kinds of solution of acquisition are mixed, then
1L is dissolved to, produces mother liquor A.
MgSO in the mother liquor B4·4H2O concentration is 24.3g/L, ZnSO4·7H2O concentration is 8.6g/L, H3BO3Concentration is
6.2g/L, KI concentration are 0.82g/L, NeMoO4·2H2O concentration is 0.25g/L, CuSO4·5H2O concentration be 0.025g/L,
CoCl·6H2O concentration is 0.025g/L；The compound method of the mother liquor B is：Take MgSO4·4H2O24.3g、ZnSO4·
7H2O8.6g、H3BO3 6.2g、KI0.82g、NeMoO4·2H2O0.25g、CuSO4·5H2O0.025g、CoCl·6H2O0.025g
It is dissolved in 800mL distilled water, treats that above-mentioned substance is dissolved completely in distilled water, then be dissolved to 1000mL, produces mother liquor B.
Thiamine concentration is 0.5g/L in the mother solution C, pyridoxol concentration is 0.4g/L, Nicotinic 0.5g/L, sweet
Propylhomoserin concentration is 2g/L；The compound method of the mother solution C is：Take thiamine 0.5g, pyridoxol 0.4g, nicotinic acid 0.5g, glycine
2g is dissolved in 800mL distilled water, is dissolved to 1000mL again after above-mentioned substance is dissolved completely in water, is produced mother solution C.
The mother liquor D is the inositol solution that mass concentration is 10g/L.
FeSO in the mother liquor E4·7H2O concentration is 5.4g/L, Na2- EDTA concentration is 7.4g/L.The mother liquor E's matches somebody with somebody
Method processed is：Take FeSO4·7H2O2.7g is dissolved in 400mL distilled water, adds Na2- EDTA3.7g is heated to 100 DEG C of dissolvings
6min, treat that above-mentioned substance is dissolved completely in water, then be dissolved to 500mL, produce mother liquor E.
The plant growth regulator is by 6-benzyl aminopurine, methyl α-naphthyl acetate and Lychee juice with 5：1.5：2 weight is than preparing
Form, the plant growth regulator is subculture reagent, and the nutrient solution for adding plant growth regulator preparation is squamous subculture liquid.
The plant growth regulator is by heteroauxin, Vc, Lychee juice with 110：1.5：190 weight ratio is formulated,
The plant growth regulator is reagent of taking root, and the nutrient solution for adding plant growth regulator preparation is nutrient solution of taking root.
The oxidation micro silver powder is the nano powder of silver oxide.
The activated carbon micro mist is the nano powder of activated carbon.
Comparative example one
Without oxidation micro silver powder and activated carbon micro mist in nutrient solution, other components are as embodiment three.
Comparative example two
Activated carbon micro mist is free of in nutrient solution, other components are as embodiment three.
Comparative example three
Without oxidation micro silver powder in nutrient solution, other components are as embodiment three.
Comparative example four
Lychee juice is free of in nutrient solution, other components are as embodiment three.
The nutrient solution of above-mentioned preparation is used for carry out tissue cultures to paper mulberry, using conventional culture methods, that is, gathers paper mulberry
The explant of tissue cultures, gather the spray with axillary bud；The explant of taken outdoors is cleaned and sterilized；It is sterile-processed
Explant callus induction afterwards；Callus break up again and squamous subculture；Detoxic seedling culture of rootage；Hardening；Crop field kind
Plant.It the results are shown in Table 1：
Table 1
As it can be seen from table 1 addition oxidation micro silver powder and activated carbon micro mist in nutrient solution, can effectively absorb culture
Phenols and quinones harmful substance in liquid, the generation for situations such as avoiding vitrifying, yellow, browning, variation, low appreciation rate,
And can also help to decompose the metabolite in culture, and effectively suppress pollution caused by explant endophyte class；Addition
Lychee juice promotes the growth of paper mulberry adventitious bud, and breeding coefficient is high, and root induction while propagation.
Claims (10)
1. a kind of paper mulberry tissue culture nutrient solution, A80 containing mother liquor~120g, mother liquor B0.8~1.2g, mother solution C 0.8 in every liter of nutrient solution
~1.2g, mother liquor D8~12g, mother liquor E3~7g, 0.8~1.2g of plant growth regulator, 25~35g of sucrose, agar powder 5~
6g, it is characterised in that：Also comprising 3~5g of oxidation micro silver powder, 3~5g of activated carbon micro mist in every liter of nutrient solution；
CaCl in the mother liquor A2·2H2O concentration is 4~5g/L, KNO3Concentration is 18~20g/L, MgSO4·7H2O concentration is 3
~4g/L, NH4NO3Concentration is 16~17g/L, KH2PO4Concentration is 1~2g/L；
MgSO in the mother liquor B4·4H2O concentration is 24~25g/L, ZnSO4·7H2O concentration is 8~9g/L, H3BO3Concentration is
6.0~6.5g/L, KI concentration are 0.8~0.9g/L, NeMoO4·2H2O concentration is 0.2~0.3g/L, CuSO4·5H2O concentration
For 0.02~0.03g/L, CoCl6H2O concentration is 0.02~0.03g/L；
Thiamine concentration is 0.4~0.6g/L in the mother solution C, pyridoxol concentration is 0.3~0.5g/L, Nicotinic 0.4
~0.6g/L, glycine concentration are 1~3g/L；
The mother liquor D is the inositol solution that mass concentration is 8~12g/L；
FeSO in the mother liquor E4·7H2O concentration is 5~6g/L, Na2- EDTA concentration is 7~8g/L.
2. paper mulberry tissue culture nutrient solution as claimed in claim 1, it is characterised in that：Every liter of A100g containing mother liquor in the nutrient solution,
Mother liquor B1g, mother solution C 1g, mother liquor D10g, mother liquor E5g, plant growth regulator 1g, sucrose 30g, agar powder 5.5g, silver oxide are micro-
Powder 4g, activated carbon micro mist 4g.
3. paper mulberry tissue culture nutrient solution as claimed in claim 1 or 2, it is characterised in that：The plant growth regulator is by 6- benzyls
Adenine phosphate, methyl α-naphthyl acetate and Lychee juice are with (4~7)：(1~2)：The weight ratio of (1.5~2.5) is formulated.
4. paper mulberry tissue culture nutrient solution as claimed in claim 1 or 2, it is characterised in that：The plant growth regulator is by indoles
Acetic acid, Vc, Lychee juice are with (100~120)：(1~2)：The weight ratio of (180~200) is formulated.
5. paper mulberry tissue culture nutrient solution as claimed in claim 1, it is characterised in that：CaCl in the mother liquor A2·2H2O concentration is
4.4g/L、KNO3Concentration is 19g/L, MgSO4·7H2O concentration is 3.8g/L, NH4NO3Concentration is 16.5g/L, KH2PO4Concentration is
1.8g/L。
6. paper mulberry tissue culture nutrient solution as claimed in claim 1, it is characterised in that：MgSO in the mother liquor B4·4H2O concentration is
24.3g/L、ZnSO4·7H2O concentration is 8.6g/L, H3BO3Concentration is that 6.2g/L, KI concentration are 0.82g/L, NeMoO4·2H2O
Concentration is 0.25g/L, CuSO4·5H2O concentration is 0.025g/L, CoCl6H2O concentration is 0.025g/L.
7. paper mulberry tissue culture nutrient solution as claimed in claim 1, it is characterised in that：Thiamine concentration is 0.5g/ in the mother solution C
L, pyridoxol concentration be 0.4g/L, Nicotinic 0.5g/L, glycine concentration 2g/L.
8. paper mulberry tissue culture nutrient solution as claimed in claim 1, it is characterised in that：The mother liquor D is that mass concentration is 10g/L
Inositol solution.
9. paper mulberry tissue culture nutrient solution as claimed in claim 1, it is characterised in that：FeSO in the mother liquor E4·7H2O concentration is
5.4g/L、Na2- EDTA concentration is 7.4g/L.
10. paper mulberry tissue culture nutrient solution as claimed in claim 1, it is characterised in that：The plant growth regulator is by indoles second
Acid, Vc, Lychee juice are with (100~120)：(1~2)：The weight ratio of (180~200) is formulated.
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| CN113100064A (en) * | 2021-05-10 | 2021-07-13 | 刘尚文 | Culture medium for tissue culture of broussonetia papyrifera and preparation method |
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| CN101697710A (en) * | 2009-11-09 | 2010-04-28 | 浙江传化生物技术有限公司 | Culture medium for tissue culture of asexual propagation of butterfly orchid |
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| CN101697710A (en) * | 2009-11-09 | 2010-04-28 | 浙江传化生物技术有限公司 | Culture medium for tissue culture of asexual propagation of butterfly orchid |
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| 陶文丞等: "构树组培快繁技术", 《黑龙江生态工程职业学院学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113100064A (en) * | 2021-05-10 | 2021-07-13 | 刘尚文 | Culture medium for tissue culture of broussonetia papyrifera and preparation method |
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Application publication date: 20171117 |