CN107325967B

CN107325967B - Cell reactor

Info

Publication number: CN107325967B
Application number: CN201710482335.5A
Authority: CN
Inventors: 刘佐雄
Original assignee: Beijing Eternal Life Technology Co ltd
Current assignee: Beijing Eternal Life Technology Co ltd
Priority date: 2017-06-22
Filing date: 2017-06-22
Publication date: 2020-08-25
Anticipated expiration: 2037-06-22
Also published as: CN107325967A

Abstract

The invention discloses a cell reactor which is characterized by comprising a tank body, a stirrer arranged in the tank body and a driving mechanism for driving the stirrer, wherein a vent pipe communicated with the inside and the outside of the tank body is arranged on the tank body, the outer end of the vent pipe is connected to an air source, one end of the vent pipe positioned in the tank body is connected with a closed fiber pipe, and the fiber pipe is communicated with the vent pipe. The invention solves the problem that the prior cell reactor generates bubbles due to aeration to cause cell damage, thereby achieving the effect of improving the cell density.

Description

Cell reactor

Technical Field

The invention relates to a cell culture device, in particular to a cell reactor.

Background

When animal or plant cells are cultured in vitro, the cell reactor is a key device of the whole culture process, and provides a proper growth environment for the cells, so that the cells can rapidly proliferate and form a required biological tissue product.

In the prior art, the cell reactor needs to be aerated to provide a proper growing environment for cells, and the aeration mode for the cell reactor is generally two modes, namely that the aeration is directly carried out in the reactor through a pipeline, and a closed pipeline is arranged in the reactor, and then small holes are drilled on the closed pipeline for aeration, wherein the pipeline is also called a distributor.

Both of the above methods of introducing gas into the reactor tend to generate bubbles in the reactor, and the bubbles in the cell culture solution are broken to cause cell damage and even death, thereby affecting the cell density in the reactor. In particular, animal cells are more harmed because they are not protected by a cell wall.

Disclosure of Invention

Aiming at the defects in the prior art, the invention aims to provide a cell reactor which reduces the generation of bubbles in a cell culture solution in a fiber tube air inlet mode, thereby reducing the damage to cells and improving the cell density.

In order to achieve the purpose, the invention provides the following technical scheme: the utility model provides a cell reactor, includes a jar body, sets up agitator in the jar body, is used for the drive the actuating mechanism of agitator, be provided with the internal and external breather pipe of intercommunication jar on the jar body, the outer end of breather pipe is connected to the air supply, the breather pipe is located jar internal one end and is connected with confined fibre pipe, the fibre pipe with the breather pipe intercommunication.

By adopting the technical scheme, when the vent pipe is used for ventilating the tank body, gas can enter the cell culture solution in the tank body through the fiber pipe, and as the inner diameter of the fiber pipe is about 200 mu m, the wall thickness is 50-70 mu m, and the pipe wall is a porous membrane, small molecules such as O2 and CO2 can freely permeate the membrane for diffusion, cells in the cell culture solution can conveniently obtain oxygen. And when the gas enters the cell culture solution through the fiber tube wall, no bubbles are generated, so that the damage to cells is reduced.

Preferably, the driving mechanism is connected with the fiber pipe through a linkage mechanism and drives the fiber pipe to linearly reciprocate in the tank body.

By adopting the technical scheme, the fiber tube can move in the cell culture solution in the tank body, on one hand, the movement plays a role in further stirring the cell culture solution, so that the density of cells in the culture solution is more uniform, and the propagation of the cells is more facilitated; on the other hand, the removal of fiber tube can let in each position in the cell culture liquid with gas for gaseous the letting in is more even, and then makes the cell of each part in the cell culture liquid sealed relatively even, thereby is favorable to the reproduction of cell more.

Preferably, the linkage mechanism comprises a reciprocating screw rod coaxially and fixedly connected with a rotating shaft of the driver and a sliding block sleeved on the reciprocating screw rod, the fiber tube is fixedly connected to the sliding block, and the tank body is further provided with a guide part for linear movement of the fiber tube.

Through adopting above-mentioned technical scheme, the driver can drive reciprocal lead screw when rotating, and need not increase extra power supply again and drive the fibre pipe and remove, has saved the manufacturing cost of this cell reactor. The rotation of the reciprocating screw rod drives the sliding block to reciprocate in the linear direction, and under the guiding action of the guiding piece, the fiber tube can linearly reciprocate along the axial direction of the reciprocating screw rod.

Preferably, the guide member is the vent pipe, the vent pipe is a hard pipe, and the vent pipe is connected to the tank body in a sliding manner.

Through adopting above-mentioned technical scheme, utilize the rigidity of breather pipe itself, play the guide effect to the fiber tube for the breather pipe not only plays the effect of ventilating, can also play the effect of removal direction.

Preferably, the guide piece is a guide pipe connected to the tank body in a sliding manner, and the vent pipe is arranged in the guide pipe.

Through adopting above-mentioned technical scheme, the breather pipe can be the hose also can be the stereoplasm pipe fitting, and at this moment, the breather pipe can be protected in the stand pipe.

Preferably, a cooling pipe is further arranged in the guide pipe.

Through adopting above-mentioned technical scheme, the cooling tube can move along with the stand pipe removes in cell culture liquid, and then can carry out stable regulation to cell culture liquid, avoids the high temperature in the cell culture liquid and causes the injury to the cell. In addition, because cooling tube and breather pipe all set up in the stand pipe, so the cooling tube can also be to the gas in the breather pipe cooling, and after the gas after the cooling entered into the cell culture liquid, can lower the temperature to the cell culture liquid on a wider scale, and its cooling effect is better.

Preferably, a sealing block is arranged on the tank body, and the guide piece penetrates through the sealing block.

Through adopting above-mentioned technical scheme for the leakproofness of jar body in the guide member joint of sliding is better.

Preferably, a storage cavity is formed in the sealing block, and a through hole for the guide piece to penetrate through is formed in the bottom wall of the storage cavity.

Through adopting above-mentioned technical scheme, can deposit sealing liquid in the storage chamber to make the leakproofness of guide and perforation junction better.

Preferably, the opening of the storage chamber is sealed by an end cap and the guide passes through the end cap.

Through adopting above-mentioned technical scheme, sealed piece diapire and end cover can be passed in proper order to the guide to make the stability of guide when sliding better.

Preferably, the end cap is threadably connected to the storage chamber.

Through adopting above-mentioned technical scheme, conveniently open the end cover to can add sealing liquid, and, the end cover is when closed, owing to adopt with storage chamber threaded connection, so, its leakproofness is better.

In summary, compared with the prior art, the invention has the following beneficial effects: 1. the invention has low possibility of generating bubbles when ventilating into the cell culture solution, and can reduce the damage to cells; 2. when the cell culture solution is aerated, the diffusion range is wider, and the uniformity is better; 3. the invention adopts a single driving source to realize the stirring of the stirrer and the movement of the fiber tube, thereby reducing the manufacturing cost; 4. the invention has better cooling effect on the cell culture solution, and is more beneficial to the propagation of cells; 5. the invention has better sealing performance.

Drawings

FIG. 1 is a schematic overall structure diagram according to a first embodiment;

FIG. 2 is a schematic structural view of the inside of a guide tube according to a second embodiment;

fig. 3 is a schematic overall structure diagram of the third embodiment.

In the figure: 1. a base; 11. a strut; 2. a tank body; 21. a discharging port; 22. a discharge port; 3. a drive mechanism; 31. a motor; 32. a small belt pulley; 33. a large belt pulley; 34. a belt; 4. a stirrer; 41. a rotating shaft; 42. a stirring impeller; 5. a linkage mechanism; 51. a reciprocating screw rod; 52. a slider; 6. a fiber tube; 7. a guide tube; 71. a breather pipe; 711. a hose; 72. feeding a pipe; 73. returning the pipe; 8. a sealing block; 81. a storage chamber; 82. and (4) end covers.

Detailed Description

The present invention will be described in further detail with reference to the accompanying drawings.

The first embodiment is as follows: a cell reactor, as shown in figure 1, comprises a base 1 and a tank 2, wherein the tank 2 is supported on the base 1 through a plurality of support rods 11. Tank body 2 top is provided with drain hole 21, and the bottom is provided with discharge gate 22, and inside is provided with agitator 4. The base 1 is provided with a driving mechanism 3 for driving the stirrer 4 to rotate.

The stirrer 4 comprises a rotating shaft 41 and a stirring impeller 42 fixedly connected to the rotating shaft 41, and the rotating shaft 41 is connected with the tank 2 through a bearing. The driving mechanism 3 comprises a motor 31, a small belt wheel 32 fixedly connected to the end of the output shaft of the motor 31, a large belt wheel 33 fixedly connected to the other end of the rotating shaft 41, and a belt 34 for connecting the small belt wheel 32 and the large belt wheel 33, wherein the motor 31 rotates to drive the small belt wheel 32 to rotate, the large belt wheel 33 is driven to rotate through the transmission of the belt 34, and then the rotating shaft 41 is driven to rotate and the stirring impeller 42 is driven to rotate. Since the small belt wheel 32 and the large belt wheel 33 cooperate to achieve a certain deceleration of the agitator 4, a speed reducer may be added to the driving mechanism 3 to achieve the deceleration.

The tank body 2 is also connected with an air pipe 71 in a sliding manner, the air pipe 71 is a hard pipe fitting, one end of the air pipe 71, which is positioned outside the tank body 2, is connected with a hose 711, the hose 711 is connected to an air source (an air pump), the other end of the air pipe 71 is connected with a fiber pipe 6, the inner diameter of the fiber pipe 6 is about 200 mu m, the wall thickness is 50-70 mu m, the pipe wall is a porous film, and small molecules such as O2 and CO2 can freely permeate through the film for. The fiber tube 6 is linearly moved in the tank body 2 through the linkage mechanism 5, the linkage mechanism 5 comprises a reciprocating screw rod 51 and a slide block 52, one end of the reciprocating screw rod is fixedly connected with the rotating shaft 41, the other end of the reciprocating screw rod is connected with the inner wall of the tank body 2 through a bearing, the slide block 52 is connected to the reciprocating screw rod 51, and the fiber tube 6 is fixedly connected to the side face of the slide block 52.

At the joint of the vent pipe 71 and the tank body 2, a sealing block 8 is arranged on the tank body 2, the sealing block 8 is made of rubber material, a storage cavity 81 is arranged on the sealing block 8, and sealing liquid, such as cell culture liquid, can be stored in the storage cavity 81. An end cap 82 is attached to the opening of the storage chamber 81, and the end cap 82 is threadedly attached to the side wall of the storage chamber 81. The vent pipe 71 passes through the end cover 82 and the bottom wall of the sealed cavity in sequence to realize sliding connection with the tank body 2.

When the cell reactor works, the motor 31 drives the rotating shaft 41 to rotate to drive the stirring impeller 42 to stir the cell culture solution, and can drive the reciprocating screw rod 51 to rotate, so that the sliding block 52 is driven to do linear reciprocating motion along the axial direction of the reciprocating screw rod 51, and the sliding block 52 drives the fiber tube 6 to do linear reciprocating motion synchronously. In the moving process of the fiber pipe 6, the air pipe 71 continuously enters air into the fiber pipe 6, and the air pipe 71 is connected with the tank body 2 in a sliding way to guide the movement of the fiber pipe 6.

Example two: a cell reactor is different from the first embodiment in that a guide tube 7 is directly connected to a fiber tube 6, as shown in FIG. 2, a vent tube 71 is provided in the guide tube 7, wherein the vent tube 71 may be a hard tube or a soft tube, and a cooling tube is further provided in the guide tube 7, and the cooling tube includes an inlet tube 72 and a return tube 73 in a U shape in the guide tube 7.

Example three: a cell reactor is different from the second embodiment in that, as shown in FIG. 3, a fiber tube 6 is ring-shaped and is arranged around the side of a slide block 52, so that the gas outlet rate of the fiber tube 6 is higher and the gas distribution in the cell culture solution is more uniform.

The above description is only a preferred embodiment of the present invention, and the protection scope of the present invention is not limited to the above embodiments, and all technical solutions belonging to the idea of the present invention belong to the protection scope of the present invention. It should be noted that modifications and embellishments within the scope of the invention may occur to those skilled in the art without departing from the principle of the invention, and are considered to be within the scope of the invention.

Claims

1. The utility model provides a cell reactor, includes jar body (2), sets up agitator (4) in jar body (2), is used for driving actuating mechanism (3) of agitator (4), its characterized in that: the driving mechanism (3) is connected with the fiber pipe (6) through a linkage mechanism (5) and drives the fiber pipe (6) to linearly reciprocate in the tank body (2), the linkage mechanism (5) comprises a reciprocating screw rod (51) coaxially and fixedly connected with a rotating shaft (41) of the driver and a sliding block (52) sleeved on the reciprocating screw rod (51), the fiber tube (6) is fixedly connected to the sliding block (52), the tank body (2) is also provided with a guide piece for the linear movement of the fiber tube (6), the tank body (2) is provided with a vent pipe (71) which is communicated with the inside and the outside of the tank body (2), the outer end of breather pipe (71) is connected to the air supply, the one end that breather pipe (71) is located jar body (2) is connected with confined fibre pipe (6), fibre pipe (6) with breather pipe (71) intercommunication.

2. A cell reactor according to claim 1, wherein: the guide piece is the breather pipe (71), the breather pipe (71) is a hard pipe fitting, and the breather pipe (71) is connected on the tank body (2) in a sliding manner.

3. A cell reactor according to claim 1, wherein: the guide piece is a guide pipe (7) connected to the tank body (2) in a sliding mode, and the vent pipe (71) is arranged in the guide pipe (7).

4. A cell reactor as claimed in claim 3, wherein: and a cooling pipe is also arranged in the guide pipe (7).

5. A cell reactor according to claim 2 or 3, wherein: the tank body (2) is provided with a sealing block (8), and the guide piece penetrates through the sealing block (8).

6. A cell reactor according to claim 5, wherein: a storage cavity (81) is formed in the sealing block (8), and a through hole for the guide piece to penetrate through is formed in the bottom wall of the storage cavity (81).

7. A cell reactor according to claim 6, wherein: the opening of the storage chamber (81) is sealed by an end cap (82), and the guide passes through the end cap (82).

8. A cell reactor according to claim 7, wherein: the end cap (82) is in threaded connection with the storage chamber (81).