CN107318983A - Milk powder containing phosphatidylserine and preparation method thereof - Google Patents
Milk powder containing phosphatidylserine and preparation method thereof Download PDFInfo
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- CN107318983A CN107318983A CN201710446287.4A CN201710446287A CN107318983A CN 107318983 A CN107318983 A CN 107318983A CN 201710446287 A CN201710446287 A CN 201710446287A CN 107318983 A CN107318983 A CN 107318983A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/152—Milk preparations; Milk powder or milk powder preparations containing additives
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/152—Milk preparations; Milk powder or milk powder preparations containing additives
- A23C9/1526—Amino acids; Peptides; Protein hydrolysates; Nucleic acids; Derivatives thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/152—Milk preparations; Milk powder or milk powder preparations containing additives
- A23C9/154—Milk preparations; Milk powder or milk powder preparations containing additives containing thickening substances, eggs or cereal preparations; Milk gels
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/152—Milk preparations; Milk powder or milk powder preparations containing additives
- A23C9/154—Milk preparations; Milk powder or milk powder preparations containing additives containing thickening substances, eggs or cereal preparations; Milk gels
- A23C9/1542—Acidified milk products containing thickening agents or acidified milk gels, e.g. acidified by fruit juices
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/16—Agglomerating or granulating milk powder; Making instant milk powder; Products obtained thereby
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Dispersion Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicinal Preparation (AREA)
Abstract
The invention discloses a kind of milk powder containing phosphatidylserine and preparation method thereof, the preparation method includes：Mixture A is obtained after DHA algal oil, polyoxyethylene sorbitan monoleate and water are mixed；Mixture B will be obtained after the mixing of L serines, phospholipase D and cushioning liquid, heating；Through solvent extraction after mixture A and mixture B is mixed, the phosphatidylserine rich in DHA is obtained；After phosphatidylserine obtained above, fresh cow milk, thickener, emulsifying agent, vegetable fat powder and animal protein are mixed milk powder is obtained after homogeneous and spray drying；DHA and PS is rich in obtained milk powder, passes through DHA and PS synergy, it is possible to increase DHA stability, promotes human body to absorb it.
Description
Technical field
The present invention relates to field of food preparation, in particular it relates to milk powder containing phosphatidylserine and preparation method thereof.
Background technology
Phosphatidylserine (Phosphatidylserine, PS) be mammalian cell membrane phosphatide important composition into
Point, especially in brain tissue, PS can successfully pass through blood-brain barrier, and a few minutes after being absorbed can enter intracerebral, its
It is closely related with the brain function such as cognition, mood, study, memory, it has been applied to improve Alzheimer disease, treatment at present depressed
In terms of disease, alleviation stress and physical fatigue.Docosahexaenoic acid (docosahexaenoicacid, DHA) is in brain tonic
There is remarkable effect in terms of intelligence development and protection eyesight, free DHA is unstable, oxidizable, and independent take can increase stomach and intestine
Burden.How DHA and PS to be synthesized, and be applied in food manufacture field, further to improve human body to DHA's and PS
The problem of absorption is urgent need to resolve of the present invention.
The content of the invention
It is rich in obtained milk powder it is an object of the invention to provide a kind of milk powder containing phosphatidylserine and preparation method thereof
Containing DHA and PS, pass through DHA and PS synergy, it is possible to increase DHA stability, promote human body to absorb it.
To achieve these goals, it is described the invention provides a kind of preparation method of the milk powder containing phosphatidylserine
Preparation method includes：
(1) mixture A is obtained after mixing DHA algal oil, polyoxyethylene sorbitan monoleate and water；
(2) mixture B will be obtained after the mixing of Serine, phospholipase D and cushioning liquid, heating；
(3) through solvent extraction after mixture A and mixture B is mixed, the phosphatidylserine rich in DHA is obtained；
(4) phosphatidylserine, fresh cow milk, thickener, emulsifying agent, vegetable fat powder and the animal protein for obtaining step (3)
After mixing milk powder is obtained after homogeneous and spray drying.
Present invention also offers a kind of milk powder containing phosphatidylserine, the milk powder is made by above-mentioned preparation method.
By above-mentioned technical proposal, the invention provides a kind of milk powder containing phosphatidylserine and preparation method thereof, institute
Stating preparation method includes：Mixture A is obtained after DHA algal oil, polyoxyethylene sorbitan monoleate and water are mixed；By Serine, phospholipase D and
Mixture B is obtained after cushioning liquid mixing, heating；Through solvent extraction after mixture A and mixture B is mixed, obtain being rich in DHA
Phosphatidylserine；By phosphatidylserine obtained above, fresh cow milk, thickener, emulsifying agent, vegetable fat powder and animal protein
After mixing milk powder is obtained after homogeneous and spray drying；It is oxidizable because free DHA is unstable, and individually take and can increase
Stomach and intestine are born, and by the synthesis to DHA and PS, are increased DHA stability, by both synergies, can be promoted human body
Absorption to DHA and PS in milk powder so that obtained milk powder possesses alleviation stress and physical fatigue, the work(of brain tonic and intelligence development
Energy.
Other features and advantages of the present invention will be described in detail in subsequent embodiment part.
Embodiment
The embodiment to the present invention is described in detail below.It should be appreciated that described herein specific
Embodiment is merely to illustrate and explain the present invention, and is not intended to limit the invention.
The end points and any value of disclosed scope are not limited to the accurate scope or value herein, these scopes or
Value should be understood to comprising the value close to these scopes or value.For number range, between the endpoint value of each scope, respectively
It can be combined with each other between the endpoint value of individual scope and single point value, and individually between point value and obtain one or more
New number range, these number ranges should be considered as specific open herein.
The invention provides a kind of preparation method of the milk powder containing phosphatidylserine, the preparation method includes：
(1) mixture A is obtained after mixing DHA algal oil, polyoxyethylene sorbitan monoleate and water；
(2) mixture B will be obtained after the mixing of Serine, phospholipase D and cushioning liquid, heating；
(3) through solvent extraction after mixture A and mixture B is mixed, the phosphatidylserine rich in DHA is obtained；
(4) phosphatidylserine, fresh cow milk, thickener, emulsifying agent, vegetable fat powder and the animal protein for obtaining step (3)
After mixing milk powder is obtained after homogeneous and spray drying.
The present invention it is a kind of preferred embodiment in, in order to further improve suction of the human body to DHA in milk powder and PS
Receive, relative to the water of 100 parts by weight, the consumption of the DHA algal oil is 0.2-0.6 parts by weight, the consumption of the polyoxyethylene sorbitan monoleate
For 2-8 parts by weight, relative to the cushioning liquid of 100 parts by weight, the consumption of the Serine is 10-20 parts by weight, the phosphorus
The consumption of lipase D is 0.5-5.5 parts by weight；
Relative to the fresh cow milk of 100 parts by weight, the consumption of the phosphatidylserine is 2-6 parts by weight, the thickener
Consumption be 2-4 parts by weight, the consumption of the emulsifying agent is 1-3 parts by weight, and the consumption of the vegetable fat powder is 0.5-0.8 weight
Part, the consumption of the animal protein is 2-3 parts by weight.
The present invention it is a kind of preferred embodiment in, in order to further improve DHA and PS combined coefficient, step
(3) mixture A and mixture B is 1 according to volume ratio in:20-40 is mixed.
The present invention it is a kind of preferred embodiment in, in order that obtained phosphatidylserine purity is higher, institute
Cushioning liquid is stated to be made up of chloroform and ethanol, and the volume ratio of chloroform and ethanol is 1:2-5.
The present invention it is a kind of preferred embodiment in, in order that obtained phosphatidylserine purity is higher, step
Suddenly the temperature of heating is 40-60 DEG C in (2), and the time of heating is 4-8h.
The present invention it is a kind of preferred embodiment in, in order to obtain purity higher phosphatidyl rich in DHA thread ammonia
Acid, the solvent includes ethanol, ether and water；And relative to the water of 100 parts by weight, the consumption of the ethanol is 20-40 weight
Part, the consumption of the ether is 10-20 parts by weight.
The present invention it is a kind of preferred embodiment in, in order to increase the viscosity of milk powder, further increase milk powder
Mouthfeel, the thickener is the one or more in xanthans, gelatin and agar.
The present invention it is a kind of preferred embodiment in, in order to further increase the mouthfeel of milk powder, the emulsifying agent is
Succinylated monoglyceride or glycerine diacetyl tartaric acid monoester.
The present invention it is a kind of preferred embodiment in, in order to further improve the mouthfeel of milk powder, the pressure of the homogeneous
Power is 15-20Mpa, and the pressure of the spray drying is 0.5-0.8Mpa.
Present invention also offers a kind of milk powder containing phosphatidylserine, the milk powder is made by above-mentioned preparation method.
The present invention will be described in detail by way of examples below.In following examples, DHA algal oil is the azure life in Qingdao
The commercially available product that thing Group Co., Ltd provides；The commercially available product that polyoxyethylene sorbitan monoleate provides for Science and Technology Ltd. of Beijing Lingbao City；L-
The commercially available product that propylhomoserin provides for the outstanding Bioisystech Co., Ltd in Shanghai three；Phospholipase D fills in the city that (China) company provides admittedly for moral
Sell product.
Embodiment 1
Mixture A is obtained after 0.2gDHA algae oils, 2g polyoxyethylene sorbitan monoleates and 100g water are mixed；By 10gL- serines,
(cushioning liquid is made up of chloroform and ethanol, and the volume ratio of chloroform and ethanol is 1 for 0.5g phospholipase Ds and 100g cushioning liquid:2)
Mixture B is obtained after mixing, heating (temperature of heating is 40 DEG C, and the time of heating is 4h)；Mixture A and mixture B are mixed
(mixture A and mixture B are 1 according to volume ratio for conjunction:20 are mixed) by solvent (solvent by 20g ethanol, 10g ether and
100g water is mixed to get) extraction, obtain the phosphatidylserine rich in DHA；By phosphatidylserine 2g obtained above, fresh ox
Through homogeneous and spray drying after newborn 100g, xanthans 2g, succinylated monoglyceride 1g, vegetable fat powder 0.5g and animal protein 2g mixing
(pressure of homogeneous is 15Mpa；The pressure of spray drying be 0.5Mpa) after obtain milk powder A1.
Embodiment 2
Mixture A is obtained after 0.6gDHA algae oils, 8g polyoxyethylene sorbitan monoleates and 100g water are mixed；By 20gL- serines,
(cushioning liquid is made up of chloroform and ethanol, and the volume ratio of chloroform and ethanol is 1 for 5.5g phospholipase Ds and 100g cushioning liquid:5)
Mixture B is obtained after mixing, heating (temperature of heating is 60 DEG C, and the time of heating is 8h)；Mixture A and mixture B are mixed
(mixture A and mixture B are 1 according to volume ratio for conjunction:40 are mixed) by solvent (solvent by 40g ethanol, 20g ether and
100g water is mixed to get) extraction, obtain the phosphatidylserine rich in DHA；By phosphatidylserine 4g obtained above, fresh ox
Through homogeneous and spray after newborn 100g, gelatin 3g, glycerine diacetyl tartaric acid monoester 2g, vegetable fat powder 0.7g and animal protein 2.5g mixing
(pressure of homogeneous is 20Mpa for mist drying；The pressure of spray drying be 0.8Mpa) after obtain milk powder A2.
Embodiment 3
Mixture A is obtained after 0.4gDHA algae oils, 5g polyoxyethylene sorbitan monoleates and 100g water are mixed；By 15gL- serines,
(cushioning liquid is made up of chloroform and ethanol, and the volume ratio of chloroform and ethanol is 1 for 3.5g phospholipase Ds and 100g cushioning liquid:3)
Mixture B is obtained after mixing, heating (temperature of heating is 50 DEG C, and the time of heating is 6h)；Mixture A and mixture B are mixed
(mixture A and mixture B are 1 according to volume ratio for conjunction:30 are mixed) by solvent (solvent by 30g ethanol, 15g ether and
100g water is mixed to get) extraction, obtain the phosphatidylserine rich in DHA；By phosphatidylserine 6g obtained above, fresh ox
Through homogeneous and spray drying after newborn 100g, agar 4g, succinylated monoglyceride 3g, vegetable fat powder 0.8g and animal protein 3g mixing
(pressure of homogeneous is 18Mpa；The pressure of spray drying be 0.6Mpa) after obtain milk powder A3.
Comparative example 1
It is prepared by the method according to embodiment 3, unlike, the water relative to 100g, the consumption of the DHA algal oil is
0.1g, the consumption of the polyoxyethylene sorbitan monoleate is 1g, the cushioning liquid relative to 100g, and the consumption of the Serine is 7g, institute
The consumption for stating phospholipase D is 0.2g；Fresh cow milk relative to 100g, the consumption of the phosphatidylserine is 1g, the agar
Consumption be 1g, the consumption of the succinylated monoglyceride is 0.5g, and the consumption of the vegetable fat powder is 0.3g, the animal protein
Consumption be 1g, finally give milk powder D1.
Comparative example 2
It is prepared by the method according to embodiment 3, unlike, the water relative to 100g, the consumption of the DHA algal oil is
0.8g, the consumption of the polyoxyethylene sorbitan monoleate is 10g, the cushioning liquid relative to 100g, and the consumption of the Serine is 25g,
The consumption of the phospholipase D is 7g；Fresh cow milk relative to 100g, the consumption of the phosphatidylserine is 8g, the agar
Consumption be 6g, the consumption of the succinylated monoglyceride is 5g, and the consumption of the vegetable fat powder is 1g, the use of the animal protein
Measure as 5g, finally give milk powder D2.
Table 1
| Embodiment is numbered | PS synthetic ratios (%) | DHA content (g/L) in milk powder |
| Embodiment 1 | 98 | 2.1 |
| Embodiment 2 | 96 | 2.4 |
| Embodiment 3 | 95 | 2.4 |
| Comparative example 1 | 81 | 1.2 |
| Comparative example 2 | 84 | 1.4 |
It can be seen that by above table data in preparation method within the scope of the present invention, PS synthetic ratio is higher, reaches
To 95-98%, DHA content is higher in obtained milk powder A1-A3, and in the preparation method outside the scope of the invention, PS conjunction
Relatively low into rate, DHA content is relatively low in obtained milk powder A1-A3.DHA and PS is rich in obtained milk powder, is passed through
DHA and PS synergy, it is possible to increase DHA stability, promotes human body to absorb it.
The preferred embodiment of the present invention described in detail above, still, the present invention are not limited in above-mentioned embodiment
Detail, in the range of the technology design of the present invention, a variety of simple variants can be carried out to technical scheme, this
A little simple variants belong to protection scope of the present invention.
It is further to note that each particular technique feature described in above-mentioned embodiment, in not lance
In the case of shield, can be combined by any suitable means, in order to avoid unnecessary repetition, the present invention to it is various can
The combination of energy no longer separately illustrates.
In addition, various embodiments of the present invention can be combined randomly, as long as it is without prejudice to originally
The thought of invention, it should equally be considered as content disclosed in this invention.
Claims (10)
1. a kind of preparation method of the milk powder containing phosphatidylserine, it is characterised in that the preparation method includes：
(1) mixture A is obtained after mixing DHA algal oil, polyoxyethylene sorbitan monoleate and water；
(2) mixture B will be obtained after the mixing of Serine, phospholipase D and cushioning liquid, heating；
(3) through solvent extraction after mixture A and mixture B is mixed, the phosphatidylserine rich in DHA is obtained；
(4) phosphatidylserine, fresh cow milk, thickener, emulsifying agent, vegetable fat powder and the animal protein mixing obtained step (3)
Milk powder is obtained by homogeneous and after being spray-dried.
2. preparation method according to claim 1, wherein, relative to the water of 100 parts by weight, the consumption of the DHA algal oil
For 0.2-0.6 parts by weight, the consumption of the polyoxyethylene sorbitan monoleate is 2-8 parts by weight, relative to the cushioning liquid of 100 parts by weight, institute
The consumption for stating Serine is 10-20 parts by weight, and the consumption of the phospholipase D is 0.5-5.5 parts by weight；
Relative to the fresh cow milk of 100 parts by weight, the consumption of the phosphatidylserine is 2-6 parts by weight, the use of the thickener
Measure as 2-4 parts by weight, the consumption of the emulsifying agent is 1-3 parts by weight, and the consumption of the vegetable fat powder is 0.5-0.8 parts by weight, institute
The consumption for stating animal protein is 2-3 parts by weight.
3. preparation method according to claim 1 or 2, wherein, mixture A and mixture B are according to volume ratio in step (3)
For 1:20-40 is mixed.
4. preparation method according to claim 3, wherein, the cushioning liquid is made up of chloroform and ethanol, and chloroform and
The volume ratio of ethanol is 1:2-5.
5. preparation method according to claim 4, wherein, the temperature of heating is 40-60 DEG C in step (2), heating when
Between be 4-8h.
6. preparation method according to claim 5, wherein, the solvent includes ethanol, ether and water；
And relative to the water of 100 parts by weight, the consumption of the ethanol is 20-40 parts by weight, and the consumption of the ether is 10-20 weights
Measure part.
7. preparation method according to claim 1 or 2, wherein, the thickener is one in xanthans, gelatin and agar
Plant or a variety of.
8. preparation method according to claim 1 or 2, wherein, the emulsifying agent is that succinylated monoglyceride or glycerine are double
Acetyltartaric acid monoester.
9. preparation method according to claim 1, wherein, the pressure of the homogeneous is 15-20Mpa, the spray drying
Pressure be 0.5-0.8Mpa.
10. a kind of milk powder containing phosphatidylserine, it is characterised in that milk powder any one institute in claim 1-9
The preparation method stated is made.
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| CN201710446287.4A CN107318983A (en) | 2017-06-14 | 2017-06-14 | Milk powder containing phosphatidylserine and preparation method thereof |
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| CN201710446287.4A CN107318983A (en) | 2017-06-14 | 2017-06-14 | Milk powder containing phosphatidylserine and preparation method thereof |
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5965413A (en) * | 1995-11-08 | 1999-10-12 | Kabushiki Kaisha Yakult Honsha | Process for producing phosphatidylserines having long chain unsaturated fatty acid as side chain |
| CN103651940A (en) * | 2013-12-05 | 2014-03-26 | 刘保惠 | Yak milk formula milk powder for middle and old age, and preparation method thereof |
| CN104004797A (en) * | 2014-06-20 | 2014-08-27 | 天津科技大学 | Method for preparing phosphatidylserine with docosahexaenoic acid at sn-2 bit |
| CN104106793A (en) * | 2013-04-18 | 2014-10-22 | 宁波大学 | Food and preparation method thereof |
| CN105360319A (en) * | 2015-10-10 | 2016-03-02 | 石家庄君乐宝乳业有限公司 | Child development formula milk powder and preparation method thereof |
| CN105400838A (en) * | 2015-12-30 | 2016-03-16 | 厦门金达威集团股份有限公司 | Method for preparing phospholipids type DHA through catalysis of biological enzyme |
| CN105494643A (en) * | 2014-09-26 | 2016-04-20 | 北京斯利安药业有限公司 | Algal oil DHA and phosphatidylserine (PS) mixed preparation product with memory improvement function |
| CN106086097A (en) * | 2016-06-08 | 2016-11-09 | 芜湖福民生物药业有限公司 | The preparation method of Phosphatidylserine |
-
2017
- 2017-06-14 CN CN201710446287.4A patent/CN107318983A/en active Pending
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|---|---|---|---|---|
| US5965413A (en) * | 1995-11-08 | 1999-10-12 | Kabushiki Kaisha Yakult Honsha | Process for producing phosphatidylserines having long chain unsaturated fatty acid as side chain |
| CN104106793A (en) * | 2013-04-18 | 2014-10-22 | 宁波大学 | Food and preparation method thereof |
| CN103651940A (en) * | 2013-12-05 | 2014-03-26 | 刘保惠 | Yak milk formula milk powder for middle and old age, and preparation method thereof |
| CN104004797A (en) * | 2014-06-20 | 2014-08-27 | 天津科技大学 | Method for preparing phosphatidylserine with docosahexaenoic acid at sn-2 bit |
| CN105494643A (en) * | 2014-09-26 | 2016-04-20 | 北京斯利安药业有限公司 | Algal oil DHA and phosphatidylserine (PS) mixed preparation product with memory improvement function |
| CN105360319A (en) * | 2015-10-10 | 2016-03-02 | 石家庄君乐宝乳业有限公司 | Child development formula milk powder and preparation method thereof |
| CN105400838A (en) * | 2015-12-30 | 2016-03-16 | 厦门金达威集团股份有限公司 | Method for preparing phospholipids type DHA through catalysis of biological enzyme |
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