CN106086097A - The preparation method of Phosphatidylserine - Google Patents
The preparation method of Phosphatidylserine Download PDFInfo
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- CN106086097A CN106086097A CN201610403742.8A CN201610403742A CN106086097A CN 106086097 A CN106086097 A CN 106086097A CN 201610403742 A CN201610403742 A CN 201610403742A CN 106086097 A CN106086097 A CN 106086097A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
- C12P7/6445—Glycerides
- C12P7/6481—Phosphoglycerides
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- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/06—Alanine; Leucine; Isoleucine; Serine; Homoserine
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Abstract
The invention discloses the preparation method of a kind of Phosphatidylserine, wherein, described preparation method includes: 1) soybean lecithin and water is mixed, prepares mixture M 1；2) in mixture M 1, add L serine and Choline phosphatase mixing, prepare Phosphatidylserine semifinished product；3) in above-mentioned prepared Phosphatidylserine semifinished product, add solvent extraction, prepare Phosphatidylserine semi-finished product；4) above-mentioned prepared Phosphatidylserine semi-finished product are sequentially passed through silicagel column and ion exchange resin, prepare Phosphatidylserine；Wherein, described solvent is made up of chloroform, ethanol, ether and water.Above-mentioned design is by first mixing soybean lecithin with water, then add L serine and Choline phosphatase reaction, the solvent containing chloroform, ethanol, ether and water is used to extract, filter through silicagel column and ion exchange resin, achieve simple to operation, and prepare Phosphatidylserine purity higher so that the effect that its range is greatly increased.
Description
Technical field
The present invention relates to the production preparation field of Phosphatidylserine, in particular it relates to the preparation side of Phosphatidylserine
Method.
Background technology
Phosphatidylserine, also known as composite nerve acid, is the active substance of cell membrane, is particularly present in brain cell, its
Major function is to improve neurocyte function, the conduction of regulation Nerve impulse, promotes brain memory function etc..Thus, at human body
In play particularly important effect.
Preparation for Phosphatidylserine now is mainly extraction method, but often the containing of Phosphatidylserine in plant
Measure less so that its extraction needs to use substantial amounts of raw material, and purity is the highest, and extracts from zooblast, the most also
The brain cell that can only use animal extracts, and not only cost of material is high, and extracting method is complicated.
Therefore it provides a kind of preparation method is relatively easy, the phosphatidyl silk ammonia that prepared Phosphatidylserine purity is higher
The preparation method of acid is the problem that the present invention needs solution badly.
Summary of the invention
For above-mentioned prior art, it is an object of the invention to overcome in prior art often Phosphatidylserine in plant
Content less so that its extraction needs to use substantial amounts of raw material, and purity is the highest, and extracts from zooblast, past
Toward also the brain cell of animal being used to extract, not only cost of material is high, and the problem that extracting method is complicated, thus provides
A kind of preparation method is relatively easy, the preparation method of the Phosphatidylserine that prepared Phosphatidylserine purity is higher.
To achieve these goals, the invention provides the preparation method of Phosphatidylserine, wherein, described preparation method
Including:
1) soybean lecithin and water are mixed, prepare mixture M 1；
2) in mixture M 1, add Serine and Choline phosphatase mixing, prepare Phosphatidylserine semifinished product；
3) in above-mentioned prepared Phosphatidylserine semifinished product, add solvent extraction, prepare Phosphatidylserine half and become
Product；
4) above-mentioned prepared Phosphatidylserine semi-finished product are sequentially passed through silicagel column and ion exchange resin, prepare phospholipid
Acyl serine；Wherein,
Described solvent is made up of chloroform, ethanol, ether and water.
By technique scheme, soybean lecithin is first mixed by the present invention with water, then adds Serine and phospholipid
Enzyme D reacts, and prepares Phosphatidylserine semifinished product, then uses above-mentioned Phosphatidylserine semifinished product containing chloroform, ethanol, second
After the solvent of ether and water extracts, filter through silicagel column and ion exchange resin, the Phosphatidylserine that prepared purity is higher,
Aforesaid operations is the most simple and convenient, and the Phosphatidylserine purity prepared is higher so that its range is greatly increased.
Other features and advantages of the present invention will be described in detail in detailed description of the invention part subsequently.
Detailed description of the invention
Hereinafter the detailed description of the invention of the present invention is described in detail.It should be appreciated that described herein specifically
Embodiment is merely to illustrate and explains the present invention, is not limited to the present invention.
The invention provides the preparation method of a kind of Phosphatidylserine, wherein, described preparation method includes:
1) soybean lecithin and water are mixed, prepare mixture M 1；
2) in mixture M 1, add Serine and Choline phosphatase mixing, prepare Phosphatidylserine semifinished product；
3) in above-mentioned prepared Phosphatidylserine semifinished product, add solvent extraction, prepare Phosphatidylserine half and become
Product；
4) above-mentioned prepared Phosphatidylserine semi-finished product are sequentially passed through silicagel column and ion exchange resin, prepare phospholipid
Acyl serine；Wherein,
Described solvent is made up of chloroform, ethanol, ether and water.
Above-mentioned design, by first being mixed with water by soybean lecithin, then adds Serine and Choline phosphatase reaction, prepares
Phosphatidylserine semifinished product, more above-mentioned Phosphatidylserine semifinished product is used containing chloroform, ethanol, ether and the solvent of water
After extracting, filtering through silicagel column and ion exchange resin, the Phosphatidylserine that prepared purity is higher, aforesaid operations is not only
Simple and convenient, and the Phosphatidylserine purity prepared is higher so that its range is greatly increased.
The present invention one preferred embodiment in, in order to the utilization rate making raw material is higher, reduce production further
Cost, relative to the step 1 of 100 weight portions) in described water, the consumption of described soybean lecithin is 30-50 weight portion, described
The consumption of Serine is 15-20 weight portion, and the consumption of described Choline phosphatase is 1-5 weight portion, and the consumption of described solvent is 200-
500 weight portions.
In solvent, the content of each composition can be adjusted according to actual needs, such as, preferred in the one of the present invention
In embodiment, in order to improve the purity of prepared Phosphatidylserine further, relative in the described solvent of 100 weight portions
Water, the consumption of described chloroform is 20-80 weight portion, and the consumption of described ethanol is 30-60 weight portion, and the consumption of described ether is
10-30 weight portion.
Step 2) in mixed process can be stand mixing, mixing condition can be not construed as limiting, certainly, the present invention's
In a kind of embodiment being more highly preferred to, step 2) in the mixing temperature of mixed process be 25-30 DEG C, incorporation time is 25-
35h。
In order to improve the purity of prepared Phosphatidylserine further, a kind of embodiment party being more highly preferred to of the present invention
In formula, step 3) in extraction times can be further selected as 2-4 time.
Step 4) in ion exchange resin can be Ion exchange resins type commonly used in the art, such as,
The one of the present invention preferred embodiment in, step 4) described in ion exchange resin can be further selected as cation hand over
Change resin.
Hereinafter will be described the present invention by embodiment.In following example, described soybean lecithin, described
Serine, described Choline phosphatase, described chloroform, described ethanol and described ether are conventional commercial product.
Embodiment 1
150g water, 30g chloroform, 45g ethanol and 15g ether are mixed, prepares solvent；By 30g soybean lecithin and 100g
Water mixes, and prepares mixture M 1；In mixture M 1, add 15gL-serine and 1g Choline phosphatase mixes under conditions of 25 DEG C
25h, prepares Phosphatidylserine semifinished product；200g solvent extraction is added in above-mentioned prepared Phosphatidylserine semifinished product,
Repeat above-mentioned extraction process 2 times, prepare Phosphatidylserine semi-finished product；By above-mentioned prepared Phosphatidylserine semi-finished product sequentially
By silicagel column and cation exchange resin, prepare Phosphatidylserine A1.
Embodiment 2
200g water, 160g chloroform, 120g ethanol and 60g ether are mixed, prepares solvent；By 50g soybean lecithin and
100g water mixes, and prepares mixture M 1；20gL-serine and 5g Choline phosphatase is added under conditions of 30 DEG C in mixture M 1
Mixing 35h, prepares Phosphatidylserine semifinished product；500g solvent extraction is added in above-mentioned prepared Phosphatidylserine semifinished product
Take, repeat above-mentioned extraction process 4 times, prepare Phosphatidylserine semi-finished product；By above-mentioned prepared Phosphatidylserine semi-finished product
Sequentially pass through silicagel column and cation exchange resin, prepare Phosphatidylserine A2.
Embodiment 3
200g water, 100g chloroform, 100g ethanol and 40g ether are mixed, prepares solvent；By 40g soybean lecithin and
100g water mixes, and prepares mixture M 1；18gL-serine and 3g Choline phosphatase is added under conditions of 28 DEG C in mixture M 1
Mixing 30h, prepares Phosphatidylserine semifinished product；400g solvent extraction is added in above-mentioned prepared Phosphatidylserine semifinished product
Take, repeat above-mentioned extraction process 3 times, prepare Phosphatidylserine semi-finished product；By above-mentioned prepared Phosphatidylserine semi-finished product
Sequentially pass through silicagel column and cation exchange resin, prepare Phosphatidylserine A3.
Embodiment 4
Being prepared according to the preparation method of embodiment 1, except for the difference that, the consumption of described chloroform is 20g, described ethanol
Consumption is 30g, and the consumption of described ether is 10g, prepares Phosphatidylserine A4.
Embodiment 5
Being prepared according to the preparation method of embodiment 2, except for the difference that, the consumption of described chloroform is 200g, described ethanol
Consumption be 150g, the consumption of described ether is 100g, prepare Phosphatidylserine A5.
Embodiment 6
Being prepared according to the preparation method of embodiment 1, except for the difference that, the consumption of described Serine is 10g, described phosphorus
The consumption of ESD is 0.5g, prepares Phosphatidylserine A6.
Embodiment 7
Being prepared according to the preparation method of embodiment 2, except for the difference that, the consumption of described Serine is 30g, described phosphorus
The consumption of ESD is 8g, prepares Phosphatidylserine A7.
Comparative example 1
It is prepared according to the preparation method of embodiment 3, except for the difference that, without ether, prepares Phosphatidylserine D1.
Comparative example 2
It is prepared according to the preparation method of embodiment 3, except for the difference that, changes ethanol into ether, prepare phosphatidyl silk ammonia
Acid D2.
Test case
Detect the content of Phosphatidylserine in above-mentioned prepared A1-A7, D1 and D2 respectively, the result obtained such as table 1 institute
Show.
Table 1
| Numbering | The content (%) of Phosphatidylserine |
| A1 | 85 |
| A2 | 89 |
| A3 | 86 |
| A4 | 68 |
| A5 | 69 |
| A6 | 72 |
| A7 | 70 |
| D1 | 52 |
| D2 | 46 |
The preferred embodiment of the present invention described in detail above, but, the present invention is not limited in above-mentioned embodiment
Detail, in the technology concept of the present invention, technical scheme can be carried out multiple simple variant, this
A little simple variant belong to protection scope of the present invention.
It is further to note that each the concrete technical characteristic described in above-mentioned detailed description of the invention, at not lance
In the case of shield, can be combined by any suitable means, in order to avoid unnecessary repetition, the present invention to various can
The compound mode of energy illustrates the most separately.
Additionally, combination in any can also be carried out between the various different embodiment of the present invention, as long as it is without prejudice to this
The thought of invention, it should be considered as content disclosed in this invention equally.
Claims (6)
1. the preparation method of a Phosphatidylserine, it is characterised in that described preparation method includes:
1) soybean lecithin and water are mixed, prepare mixture M 1；
2) in mixture M 1, add Serine and Choline phosphatase mixing, prepare Phosphatidylserine semifinished product；
3) in above-mentioned prepared Phosphatidylserine semifinished product, add solvent extraction, prepare Phosphatidylserine semi-finished product；
4) above-mentioned prepared Phosphatidylserine semi-finished product are sequentially passed through silicagel column and ion exchange resin, prepare phosphatidyl silk
Propylhomoserin；Wherein,
Described solvent is made up of chloroform, ethanol, ether and water.
Preparation method the most according to claim 1, wherein, relative to the step 1 of 100 weight portions) in described water, described
The consumption of soybean lecithin is 30-50 weight portion, and the consumption of described Serine is 15-20 weight portion, the use of described Choline phosphatase
Amount is 1-5 weight portion, and the consumption of described solvent is 200-500 weight portion.
Preparation method the most according to claim 1 and 2, wherein, relative to the water in the described solvent of 100 weight portions, institute
The consumption stating chloroform is 20-80 weight portion, and the consumption of described ethanol is 30-60 weight portion, and the consumption of described ether is 10-30 weight
Amount part.
Preparation method the most according to claim 1 and 2, wherein, step 2) in the mixing temperature of mixed process be 25-30
DEG C, incorporation time is 25-35h.
Preparation method the most according to claim 1 and 2, wherein, step 3) in extraction times be 2-4 time.
Preparation method the most according to claim 1 and 2, wherein, step 4) described in ion exchange resin be that cation is handed over
Change resin.
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Cited By (8)
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| CN107136511A (en) * | 2017-05-25 | 2017-09-08 | 山东省科学院生物研究所 | A kind of peony seed oil composition for promoting cranial nerve development, Peony-seed-oil soft capsule and preparation method thereof |
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| CN107151683A (en) * | 2017-06-12 | 2017-09-12 | 芜湖福民生物药业股份有限公司 | The preparation method of phosphatidylserine |
| CN107318983A (en) * | 2017-06-14 | 2017-11-07 | 芜湖福民生物药业股份有限公司 | Milk powder containing phosphatidylserine and preparation method thereof |
| CN107334008A (en) * | 2017-06-14 | 2017-11-10 | 芜湖福民生物药业股份有限公司 | Fruit beverage containing phosphatidylserine and preparation method thereof |
| CN107343892A (en) * | 2017-06-14 | 2017-11-14 | 芜湖福民生物药业股份有限公司 | Traditional Chinese medicine oral liquid and preparation method thereof |
| CN110229183A (en) * | 2019-07-25 | 2019-09-13 | (株)斗山 | A kind of egg yolk lecithin production technology |
| CN111575325A (en) * | 2020-04-21 | 2020-08-25 | 富诺健康股份有限公司 | Preparation method of phosphatidylserine |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107136511A (en) * | 2017-05-25 | 2017-09-08 | 山东省科学院生物研究所 | A kind of peony seed oil composition for promoting cranial nerve development, Peony-seed-oil soft capsule and preparation method thereof |
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| CN107149146A (en) * | 2017-06-14 | 2017-09-12 | 芜湖福民生物药业股份有限公司 | Lozenge containing phosphatidylserine and preparation method thereof |
| CN107318983A (en) * | 2017-06-14 | 2017-11-07 | 芜湖福民生物药业股份有限公司 | Milk powder containing phosphatidylserine and preparation method thereof |
| CN107334008A (en) * | 2017-06-14 | 2017-11-10 | 芜湖福民生物药业股份有限公司 | Fruit beverage containing phosphatidylserine and preparation method thereof |
| CN107343892A (en) * | 2017-06-14 | 2017-11-14 | 芜湖福民生物药业股份有限公司 | Traditional Chinese medicine oral liquid and preparation method thereof |
| CN110229183A (en) * | 2019-07-25 | 2019-09-13 | (株)斗山 | A kind of egg yolk lecithin production technology |
| CN110229183B (en) * | 2019-07-25 | 2021-07-20 | (株)斗山 | Production process of egg yolk lecithin |
| CN111575325A (en) * | 2020-04-21 | 2020-08-25 | 富诺健康股份有限公司 | Preparation method of phosphatidylserine |
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